keyword
MENU ▼
Read by QxMD icon Read
search

Forensic biology

keyword
https://www.readbyqxmd.com/read/28337600/technical-note-envision%C3%A2-flex-improves-the-detectability-of-depletions-of-myoglobin-and-troponin-t-in-forensic-cases-of-myocardial-ischemia-infarction
#1
Sara Sabatasso, Catia Pomponio, Tony Fracasso
Immunohistochemistry is a well-established technique used in many research laboratories as well as in clinical diagnostics. The method allows to visualize the expression of proteins in biological tissues, as well as to evaluate this expression semi-quantitatively. For diagnosis, an optimal staining, based on a straightforward protocol, is crucial. In many sudden cardiac death cases, immunohistochemistry is the only tool enabling the diagnosis of myocardial ischemia/infarction, thus the diagnosis of the cause of death...
March 23, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28334817/stability-of-synthetic-cannabinoids-in-biological-specimens-analysis-through-liquid-chromatography-tandem-mass-spectrometry
#2
Chelsea Fort, Thomas Jourdan, Jesse Kemp, Byron Curtis
The focus of this study was to determine the stability of four synthetic cannabinoids, XLR-11, UR-144, AB-Pinaca and AB-Fubinaca in biological specimens for the purpose of casework processing prioritization. The study used human whole blood spiked with the compounds of interest to mimic real forensic laboratory samples submitted for synthetic cannabinoid analysis. The spiked whole blood specimens were incubated under one of three temperature conditions: room or ambient (22°C), refrigerated (4°C) and frozen (-20°C) for a period of 12 weeks...
February 18, 2017: Journal of Analytical Toxicology
https://www.readbyqxmd.com/read/28315050/forensic-use-of-y-chromosome-dna-a-general-overview
#3
REVIEW
Manfred Kayser
The male-specific part of the human Y chromosome is widely used in forensic DNA analysis, particularly in cases where standard autosomal DNA profiling is not informative. A Y-chromosomal gene fragment is applied for inferring the biological sex of a crime scene trace donor. Haplotypes composed of Y-chromosomal short tandem repeat polymorphisms (Y-STRs) are used to characterise paternal lineages of unknown male trace donors, especially suitable when males and females have contributed to the same trace, such as in sexual assault cases...
March 17, 2017: Human Genetics
https://www.readbyqxmd.com/read/28301022/use-of-an-alternate-light-source-to-detect-tooth-and-bone
#4
Geraldo Elias Miranda, Rodolfo Francisco Haltenhoff Melani, Luiz Francisquini, Eduardo Daruge
The aim of this study was to identify the combination of wavelength and filter that best detects tooth and bone, and to determine which biological materials (enamel, dental root or bone) have highest fluorescence intensity when exposed to an alternate light source (ALS). Tooth and bone samples were lighted with ALS and photographed. Adobe Photoshop™ and ImageJ™ softwares were used for image analysis. Data obtained by measuring the photograph pixels were subjected to analysis of variance. The mean values of significant effects were compared by the Tukey test...
January 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28293949/coomassie-brilliant-blue-g-250-dye-new-application-for-forensic-fingerprint-analysis
#5
Erica Brunelle, Anh Minh Le, Crystal Huynh, Kelly Wingfield, Lenka Halámková, Juliana Agudelo, Jan Halámek
The Bradford reagent, comprised of the Coomassie Brilliant Blue G-250 dye, methanol and phosphoric acid, has been traditionally used for quantifying proteins. Use of this reagent in the Bradford assay relies on the binding of the Coomassie Blue G-250 dye to proteins. However, the ability of the dye to react with a small group of amino acids - arginine, histidine, lysine, phenylalanine, tyrosine, and tryptophan - makes it a viable chemical assay for fingerprint analysis in order to identify the biological sex of the fingerprint originator...
March 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28273508/helping-to-distinguish-primary-from-secondary-transfer-events-for-trace-dna
#6
Duncan Taylor, Alex Biedermann, Lydie Samie, Ka-Man Pun, Tacha Hicks, Christophe Champod
DNA is routinely recovered in criminal investigations. The sensitivity of laboratory equipment and DNA profiling kits means that it is possible to generate DNA profiles from very small amounts of cellular material. As a consequence, it has been shown that DNA we detect may not have arisen from a direct contact with an item, but rather through one or more intermediaries. Naturally the questions arising in court, particularly when considering trace DNA, are of how DNA may have come to be on an item. While scientists cannot directly answer this question, forensic biological results can help in discriminating between alleged activities...
February 20, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28270824/advances-of-community-level-plant-dna-barcoding-in-china
#7
Nancai Pei, Bufeng Chen, W J Kress
DNA barcoding is a commonly used bio-technology in multiple disciplines including biology, environmental science, forensics and inspection, etc. Forest dynamic plots provide a unique opportunity to carry out large-scale, comparative, and multidisciplinary research for plant DNA barcoding. The paper concisely reviewed four previous progresses in China; specifically, species discrimination, community phylogenetic reconstruction, phylogenetic community structure exploration, and biodiversity index evaluation. Further, we demonstrated three major challenges; specifically, building the impetus to generate DNA barcodes using multiple plant DNA markers for all woody species at forest community levels, analyzing massive DNA barcoding sequence data, and promoting theoretical innovation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28254385/dna-methylation-based-forensic-age-prediction-using-artificial-neural-networks-and-next-generation-sequencing
#8
Athina Vidaki, David Ballard, Anastasia Aliferi, Thomas H Miller, Leon P Barron, Denise Syndercombe Court
The ability to estimate the age of the donor from recovered biological material at a crime scene can be of substantial value in forensic investigations. Aging can be complex and is associated with various molecular modifications in cells that accumulate over a person's lifetime including epigenetic patterns. The aim of this study was to use age-specific DNA methylation patterns to generate an accurate model for the prediction of chronological age using data from whole blood. In total, 45 age-associated CpG sites were selected based on their reported age coefficients in a previous extensive study and investigated using publicly available methylation data obtained from 1156 whole blood samples (aged 2-90 years) analysed with Illumina's genome-wide methylation platforms (27K/450K)...
February 26, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28252873/glucose-metabolism-in-completed-suicide-a-forensic-pathological-pilot-study
#9
Jonas Forsman, Terhi Keltanen, Benny Liberg, Antti Sajantila, Thomas Masterman, Katarina Lindroos
AIM: To determine whether antemortem blood levels of glycated hemoglobin (HbA1c) and glucose predict completed suicide and, by extension, whether markers of glucose metabolism might be associated with a prosuicidal trait or state. METHOD: From consecutively performed autopsies, samples of blood and vitreous humor from 17 suicide victims and 27 non-suicide controls were compared with regard to levels of glucose, lactate, and HbA1c. RESULTS: Mean HbA1c was higher, and mean estimated blood glucose lower, among suicide victims, although tests revealed no significant differences (P=0...
February 28, 2017: Croatian Medical Journal
https://www.readbyqxmd.com/read/28252611/-on-the-problem-of-hiv-infection-in-the-objects-of-forensic-medical-expertise
#10
P L Ivanov, T V Timoshenko, E Yu Zemskova
The authors overview the current state of research in the field of diagnostics and identification of the signs suggesting the presence of HIV in the materials obtained from the human corpses undergoing forensic medical expertise at different stages of their post-mortem changes. Another objective of the present work was to evaluate the risk of HIV infection for the medical personnel involved in the autopsy studies taking into consideration the significance attached in different countries to the problem of anti-infectious protection of the staff of the state institutions of forensic medical expertise...
2017: Sudebno-meditsinskaia Ekspertiza
https://www.readbyqxmd.com/read/28243772/diagnosis-of-aluminum-phosphide-poisoning-using-a-new-analytical-approach-forensic-application-to-a-lethal-intoxication
#11
Hui Yan, Ping Xiang, Sujing Zhang, Baohua Shen, Min Shen
Aluminum phosphide (AlP) is an effective and cheap pesticide that is commonly used worldwide, but it is also a common cause of human poisoning and carries a high mortality rate. AlP reacts with moisture in air, water, and hydrochloric acid in the stomach to produce phosphine (PH3) gas. Two routes of exposure are ingestion of AlP and inhalation of phosphine generated by the action of moisture on AlP. Absorbed phosphine is rapidly metabolized into phosphite and hypophosphite. A method is described for the analysis of the phosphine metabolites in various biological matrices...
February 27, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28214756/multifarious-applications-of-atomic-force-microscopy-in-forensic-science-investigations
#12
REVIEW
Gaurav Pandey, Maithri Tharmavaram, Deepak Rawtani, Sumit Kumar, Y Agrawal
Forensic science is a wide field comprising of several subspecialties and uses methods derived from natural sciences for finding criminals and other evidence valid in a legal court. A relatively new area; Nano-forensics brings a new era of investigation in forensic science in which instantaneous results can be produced that determine various agents such as explosive gasses, biological agents and residues in different crime scenes and terrorist activity investigations. This can be achieved by applying Nanotechnology and its associated characterization techniques in forensic sciences...
February 11, 2017: Forensic Science International
https://www.readbyqxmd.com/read/28211993/recent-lab-on-chip-developments-for-novel-drug-discovery
#13
REVIEW
Nauman Khalid, Isao Kobayashi, Mitsutoshi Nakajima
Microelectromechanical systems (MEMS) and micro total analysis systems (μTAS) revolutionized the biochemical and electronic industries, and this miniaturization process became a key driver for many markets. Now, it is a driving force for innovations in life sciences, diagnostics, analytical sciences, and chemistry, which are called 'lab-on-a-chip, (LOC)' devices. The use of these devices allows the development of fast, portable, and easy-to-use systems with a high level of functional integration for applications such as point-of-care diagnostics, forensics, the analysis of biomolecules, environmental or food analysis, and drug development...
February 17, 2017: Wiley Interdisciplinary Reviews. Systems Biology and Medicine
https://www.readbyqxmd.com/read/28197142/home-made-cost-effective-preservation-buffer-is-a-better-alternative-to-commercial-preservation-methods-for-microbiome-research
#14
Sebastian Menke, Mark A F Gillingham, Kerstin Wilhelm, Simone Sommer
The investigation of wildlife gastrointestinal microbiomes by next-generation sequencing approaches is a growing field in microbial ecology and conservation. Such studies often face difficulties in sample preservation if neither freezing facilities nor liquid nitrogen (LQN) are readily available. Thus, in order to prevent microbial community changes because of bacterial growth after sampling, preservation buffers need to be applied to samples. However, the amount of microbial community variation attributable to the different preservation treatments and potentially affecting biological interpretation is hardly known...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28176637/personalized-medicine-applied-to-forensic-sciences-new-advances-and-perspectives-for-a-tailored-forensic-approach
#15
Alessandro Santurro, Anna Maria Vullo, Marina Borro, Giovanna Gentile, Raffaele La Russa, Maurizio Simmaco, Paola Frati, Vittorio Fineschi
Personalized medicine (PM), included in P5 medicine (Personalized, Predictive, Preventive, Participative and Precision medicine) is an innovative approach to the patient, emerging from the need to tailor and to fit the profile of each individual. PM promises to dramatically impact also on forensic sciences and justice system in ways we are only beginning to understand. The application of omics (genomic, transcriptomics, epigenetics/imprintomics, proteomic and metabolomics) is ever more fundamental in the so called "molecular autopsy"...
February 7, 2017: Current Pharmaceutical Biotechnology
https://www.readbyqxmd.com/read/28164539/post-mortem-identification-of-a-fire-carbonized-body-by-str-genotyping
#16
Raluca Dumache, Camelia Muresan, Veronica Ciocan, Alexandru F Rogobete, Alexandra Enache
BACKGROUND: Identification of bodies of unknown identity that are victims of exposure to very high temperatures, resulting from fires, plane crashes, and terrorist attacks, represents one of the most difficult sides of forensic genetics, because of the advanced state of decomposition. The aim of this study was the identification of the carbonized cadaver of a fire victim through STR genotyping. METHODS: We used blood samples obtained from the iliac artery during the autopsy examination as biological samples from the unidentified victim...
October 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28160742/determination-of-rodenticides-and-related-metabolites-in-rabbit-liver-and-biological-matrices-by-liquid-chromatography-coupled-to-orbitrap-high-resolution-mass-spectrometry
#17
Marina López-García, Roberto Romero-González, Antonia Garrido Frenich
An analytical method based on ultra-high performance liquid chromatography (UHPLC) coupled to Orbitrap high resolution mass spectrometry was developed for the determination of rodenticides (bromadiolone, brodifacoum, difenacoum, chlorophacinone, diphacinone, coumachlor and warfarin) in liver matrix. Different extraction conditions were tested, obtaining the best results when the "dilute and shoot" method (acidified acetonitrile as extraction solvent) and a clean-up step with primary secondary amine (PSA) were used...
January 28, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28160051/characterization-and-identification-of-eight-designer-benzodiazepine-metabolites-by-incubation-with-human-liver-microsomes-and-analysis-by-a-triple-quadrupole-mass-spectrometer
#18
Souleiman El Balkhi, Maxime Chaslot, Nicolas Picard, Sylvain Dulaurent, Martine Delage, Olivier Mathieu, Franck Saint-Marcoux
Designer benzodiazepines (DBZDs) have become of particular importance in the past few years. The metabolite monitoring of DBZD in biological fluids could be of great interest in clinical and forensic toxicology. However, DBZD metabolites are not known or not commercially available. The identification of some DBZD metabolites has been mostly explored by self-administration studies or by in vitro studies followed by high-resolution mass spectrometry. The question arose whether a unit resolution instrument could be efficient enough to allow the identification of DBZD metabolites...
February 4, 2017: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/28144937/a-comparative-taphonomic-analysis-of-24-trophy-skulls-from-modern-forensic-cases
#19
Josephine M Yucha, James T Pokines, Eric J Bartelink
Cranial remains retained from fallen enemies are commonly referred to as "trophy skulls," and many such crania were acquired as souvenirs by U.S. servicemembers during WWII and the Vietnam conflict. These remains increasingly have become the subject of forensic anthropological analysis as their possessors, typically veterans or their relatives, try to discard or repatriate them. The present research uses a qualitative analytical approach to review 24 cases of reported trophy skulls (14 previously unpublished cases and 10 from the literature) to determine which perimortem and postmortem characteristics are most useful for generating a taphonomic profile...
February 1, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28127407/metabolites-replace-the-parent-drug-in-the-drug-arena-the-cases-of-fonazepam-and-nifoxipam
#20
REVIEW
Maria Katselou, Ioannis Papoutsis, Panagiota Nikolaou, Chara Spiliopoulou, Sotiris Athanaselis
Fonazepam (desmethylflunitrazepam) and nifoxipam (3-hydroxy-desmethylflunitrazepam) are benzodiazepine derivatives and active metabolites of flunitrazepam. They recently invaded the drug arena as substances of abuse and alerted the forensic community after being seized in powder and tablet forms in Europe between 2014 and 2016. A review of all the existing knowledge of fonazepam and nifoxipam is reported, concerning their chemistry, synthesis, pharmacology and toxicology, prevalence/use, biotransformation and their analysis in biological samples...
2017: Forensic Toxicology
keyword
keyword
73506
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"