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https://www.readbyqxmd.com/read/27908595/polymerase-chain-reaction-with-nearby-primers
#1
Ravil R Garafutdinov, Aizilya A Galimova, Assol R Sakhabutdinova
DNA analysis of biological specimens containing degraded nucleic acids such as mortal remains, archaeological artefacts, forensic samples etc. has gained more attention in recent years. DNA extracted from these samples is often inapplicable for conventional polymerase chain reaction (PCR), so for its amplification the nearby primers are commonly used. Here we report the data that clarify the features of PCR with nearby and abutting primers. We have shown that the proximity of primers leads to significant reduction of the reaction time and ensures the successful performance of DNA amplification even in the presence of PCR inhibitors...
November 28, 2016: Analytical Biochemistry
https://www.readbyqxmd.com/read/27902658/understanding-negative-predictive-value-of-diagnostic-tests-used-in-clinical-practice
#2
Reba A Umberger, Linda A Hatfield, Patricia M Speck
Nurses review, evaluate, and use diagnostic test results on a routine basis. However, the skills necessary to evaluate a particular test using statistical outcome measures is often lacking. The purpose of this article is to examine and interpret the underlying principles for use of the statistical outcomes of diagnostic screening tests (sensitivity, specificity, and positive (PPV) and negative (NPV) predictive values, with a discussion about use of SpPIn [Specificity, Positive test = rule in], and SnNOut [Sensitivity, Negative test = rule out]) in advanced nursing clinical practice...
January 2017: Dimensions of Critical Care Nursing: DCCN
https://www.readbyqxmd.com/read/27896715/genome-sequencing
#3
Mansi Verma, Samarth Kulshrestha, Ayush Puri
Genome sequencing is an important step toward correlating genotypes with phenotypic characters. Sequencing technologies are important in many fields in the life sciences, including functional genomics, transcriptomics, oncology, evolutionary biology, forensic sciences, and many more. The era of sequencing has been divided into three generations. First generation sequencing involved sequencing by synthesis (Sanger sequencing) and sequencing by cleavage (Maxam-Gilbert sequencing). Sanger sequencing led to the completion of various genome sequences (including human) and provided the foundation for development of other sequencing technologies...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27890106/study-on-the-criteria-for-assessing-skull-face-correspondence-in-craniofacial-superimposition
#4
Oscar Ibáñez, Andrea Valsecchi, Fabio Cavalli, María Isabel Huete, Blanca Rosario Campomanes-Alvarez, Carmen Campomanes-Alvarez, Ricardo Vicente, David Navega, Ann Ross, Caroline Wilkinson, Rimantas Jankauskas, Kazuhiko Imaizumi, Rita Hardiman, Paul Thomas Jayaprakash, Elena Ruiz, Francisco Molinero, Patricio Lestón, Elizaveta Veselovskaya, Alexey Abramov, Maryna Steyn, Joao Cardoso, Daniel Humpire, Luca Lusnig, Daniele Gibelli, Debora Mazzarelli, Daniel Gaudio, Federica Collini, Sergio Damas
Craniofacial superimposition has the potential to be used as an identification method when other traditional biological techniques are not applicable due to insufficient quality or absence of ante-mortem and post-mortem data. Despite having been used in many countries as a method of inclusion and exclusion for over a century it lacks standards. Thus, the purpose of this research is to provide forensic practitioners with standard criteria for analysing skull-face relationships. Thirty-seven experts from 16 different institutions participated in this study, which consisted of evaluating 65 criteria for assessing skull-face anatomical consistency on a sample of 24 different skull-face superimpositions...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27890099/potential-use-of-pericardial-ctni-mg-2-and-ca-2-in-the-forensic-investigation-of-seawater-drowning-in-greece-an-initial-assessment
#5
Aspasia Deliligka, Nikolaos Raikos, Fotios Chatzinikolaou, Ioannis Venizelos, Kyriakos Chatzopoulos, Antonis Goulas
The investigation of drowning constitutes one of the biggest problems in forensic practice. Elevated cardiac troponin I (cTnI) levels in biological fluids have been associated with myocardial damage, whereas increased Mg(2+) and Ca(2+) levels were found in cases of seawater drowning. The aim of this study was to examine the diagnostic utility of postmortem determination of cTnI, Mg(2+) and Ca(2+) in the pericardial fluid, in differentiating between cases of seawater drowning related to myocardial injury and those brought about by other causes...
November 2016: Legal Medicine
https://www.readbyqxmd.com/read/27885654/historical-trends-in-graduate-research-and-training-of-diplomates-of-the-american-board-of-forensic-anthropology
#6
Jonathan D Bethard
The history of forensic anthropology has been documented by numerous scholars. These contributions have described the work of early pioneers in the field and have described important milestones, such as the founding of the Physical Anthropology Section of the American Academy of Forensic Sciences (AAFS) in 1972 and the American Board of Forensic Anthropology (ABFA) in 1977. This paper contributes to the growing literature on the history of forensic anthropology by documenting the academic training of all individuals who have been granted diplomate status by the ABFA (n = 115)...
November 25, 2016: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27883982/exocranial-surfaces-for-sex-assessment-of-the-human-cranium
#7
Barbora Musilová, Ján Dupej, Jana Velemínská, Kathia Chaumoitre, Jaroslav Bruzek
Determination of sex is one of the most important and challenging disciplines in biological anthropology. Creating a robust tool for sexing crania is crucial for forensic anthropology, especially in this period of migration, travel, and globalization, when different populations are mixed together in one region. Many different approaches to sex estimation using the skull have been published; however, population specificity and oscillation of variable sexual dimorphism typically reduces their effectiveness. The aim of this study was to create a robust classifier using virtual anthropology without the use of a CT scanner...
November 11, 2016: Forensic Science International
https://www.readbyqxmd.com/read/27869443/-post-mortem-investigation-of-alveolar-air-in-forensic-practice
#8
Miroslav Bauer, Jiřina Bauerová, Ján Šikuta, Jozef Šidlo
Exhaled air from biological materials is used for the purpose of toxicologico-chemical analysis particularly in detecting of alcohol influence in traffic or within a working process. Similarly, still a more and more actual requirement seems to be a necessity to analyse alveolar air from necroptic material. This necessity is emphasised not only by the fact, that inhalant intoxications in the form of poisoning represent their substantial part, but also a demand to evidence exactly a noxious agent both specifically and also in at least two materials...
2016: Soudní Lékarství
https://www.readbyqxmd.com/read/27864949/multimethod-resolution-of-a-small-scale-case-of-commingling
#9
Janet E Finlayson, Eric J Bartelink, Alexandra Perrone, Kevin Dalton
This study highlights the use of multiple methods for resolving a case of commingled human remains. Skeletal remains were located in a marijuana field in rural northern California by law enforcement. Although initially buried in shallow graves, the remains of two decedents were disturbed by large carnivores and scattered over a wide area. The remains were submitted by law enforcement for forensic anthropological analysis and resolution of commingling. To segregate the remains of the two individuals, a number of methods were employed, including: (i) physical matching of fragmented remains; (ii) articulation to evaluate joint congruence; (iii) visual pair-matching of bilateral elements; (iv) osteometric pair-matching; (v) evaluation of taphonomic patterns; (vi) DNA analysis; and (vii) portable X-ray spectrometry (pXRF)...
November 16, 2016: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/27857155/fluorescence-and-magnetic-activated-cell-sorting-strategies-to-separate-spermatozoa-involving-plural-contributors-from-biological-mixtures-for-human-identification
#10
Yan Xu, Jianhui Xie, Ronghua Chen, Yu Cao, Yuan Ping, Qingwen Xu, Wei Hu, Dan Wu, Lihua Gu, Huaigu Zhou, Xin Chen, Ziqin Zhao, Jiang Zhong, Rui Li
No effective method has been developed to distinguish sperm cells originating from different men in multi-suspect sexual assault cases. Here we combined MACS and FACS to isolate single donor sperm cells from forensic mixture samples including female vaginal epithelial cells and sperm cells from multiple contributors. Sperms from vaginal swab were isolated by MACS using FITC-conjugated A kinase anchor protein 3 (AKAP3) antibody; target individual sperm cells involving two or three donors were separated by FACS using FITC-labeled blood group A/B antigen antibody...
November 18, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27834143/distribution-of-synthetic-cannabinoids-jwh-210-rcs-4-and-%C3%A2-9-tetrahydrocannabinol-after-intravenous-administration-to-pigs
#11
Nadine Schaefer, Mattias Kettner, Matthias W Laschke, Julia Schlote, Andreas H Ewald, Michael D Menger, Hans H Maurer, Peter H Schmidt
Synthetic cannabinoids (SCs) have become an increasing issue in forensic toxicology. Controlled human studies evaluating pharmacokinetic data of SCs are lacking and only few animal studies have been published. Thus, an interpretation of analytical results found in intoxicated or poisoned individuals is difficult. Therefore, the distribution of two selected SCs, namely 4-ethylnaphthalen-1-yl-(1-pentylindol-3-yl)methanone (JWH-210) and 2-(4-methoxyphenyl)-1-(1-pentyl-indol-3-yl)methanone (RCS-4) as well as ∆9-tetrahydrocannabinol (THC) as reference were examined in pigs...
November 11, 2016: Current Neuropharmacology
https://www.readbyqxmd.com/read/27819737/human-autosomal-dna-and-x-chromosome-str-profiles-obtained-from-chrysomya-albiceps-diptera-calliphoridae-larvae-used-as-a-biological-trace
#12
T C Oliveira, A B R Santos, K C N Rabelo, C A Souza, S M Santos, S Crovella
The use of insects to answer questions in criminal investigations, as well as a combination of forensic genetic techniques to obtain human DNA from the organisms, especially necrophagous dipterians, have gained ground in recent decades among researchers and professionals in this area. The objective of our study was to evaluate and compare two methods of human DNA extraction, commonly used for forensic samples, to obtain human autosomal DNA and X chromosome short tandem repeat profiles from the digestive tract of Chrysomya albiceps (Diptera: Calliphoridae) larvae...
November 3, 2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27799432/determination-of-thickness-of-thin-turbid-painted-over-layers-using-micro-scale-spatially-offset-raman-spectroscopy
#13
Claudia Conti, Marco Realini, Chiara Colombo, Alessandra Botteon, Moira Bertasa, Jana Striova, Marco Barucci, Pavel Matousek
We present a method for estimating the thickness of thin turbid layers using defocusing micro-spatially offset Raman spectroscopy (micro-SORS). The approach, applicable to highly turbid systems, enables one to predict depths in excess of those accessible with conventional Raman microscopy. The technique can be used, for example, to establish the paint layer thickness on cultural heritage objects, such as panel canvases, mural paintings, painted statues and decorated objects. Other applications include analysis in polymer, biological and biomedical disciplines, catalytic and forensics sciences where highly turbid overlayers are often present and where invasive probing may not be possible or is undesirable...
December 13, 2016: Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences
https://www.readbyqxmd.com/read/27776637/untargeted-screening-of-unknown-xenobiotics-and-potential-toxins-in-plasma-of-poisoned-patients-using-high-resolution-mass-spectrometry-generation-of-xenobiotic-fingerprint-using-background-subtraction
#14
Chang Chen, Ariane Wohlfarth, Hui Xu, Dan Su, Xin Wang, Hongliang Jiang, Yulin Feng, Mingshe Zhu
A novel analytical workflow was developed and applied for the detection and identification of unknown xenobiotics in biological samples. High-resolution mass spectrometry (HRMS)-based data-independent MS(E) acquisition was employed to record full scan MS and fragment spectral datasets of test and control samples. Then, an untargeted data-mining technique, background subtraction, was utilized to find xenobiotics present only in test samples. Structural elucidation of the detected xenobiotics was accomplished by database search, spectral interpretation, and/or comparison with reference standards...
November 9, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27769399/progressing-the-analysis-of-improvised-explosive-devices-comparative-study-for-trace-detection-of-explosive-residues-in-handprints-by-raman-spectroscopy-and-liquid-chromatography
#15
Félix Zapata, Mª Ángeles Fernández de la Ossa, Elizabeth Gilchrist, Leon Barron, Carmen García-Ruiz
Concerning the dreadful global threat of terrorist attacks, the detection of explosive residues in biological traces and marks is a current need in both forensics and homeland security. This study examines the potential of Raman microscopy in comparison to liquid chromatography (ion chromatography (IC) and reversed-phase high performance liquid chromatography (RP-HPLC)) to detect, identify and quantify residues in human handmarks of explosives and energetic salts commonly used to manufacture Improvised Explosive Devices (IEDs) including dynamite, ammonium nitrate, single- and double-smokeless gunpowders and black powder...
December 1, 2016: Talanta
https://www.readbyqxmd.com/read/27763586/-forensic-medical-implications-of-the-traumas-and-traces-of-biological-deposits-in-the-context-of-comprehensive-expertises-of-the-injuries-inflicted-inside-the-passenger-compartment-of-the-car
#16
V A Fetisov, A A Gusarov, Eksp Smirenin
The results of analysis of forensic medical expertises carried out by different state institutions of forensic medical expertise of this country are presented. It was shown that the practice of standardized expertises of the injuries inflicted inside the passenger compartment of the car remains to be established. The comprehensive approach to the solution of the problem of the circumstances of the road traffic accident and the positions of its participants in the passenger compartment of the car is possible based only on the multi-faceted investigation...
2016: Sudebno-meditsinskaia Ekspertiza
https://www.readbyqxmd.com/read/27758125/the-novel-primers-for-mammal-species-identification-based-mitochondrial-cytochrome-b-sequence-implication-for-reserved-wild-animals-in-thailand-and-endangered-mammal-species-in-southeast-asia
#17
Yuttamol Muangkram, Worawidh Wajjwalku, Akira Amano, Manakorn Sukmak
We presented the powerful techniques for species identification using the short amplicon of mitochondrial cytochrome b gene sequence. Two faecal samples and one single hair sample of the Asian tapir were tested using the new cytochrome b primers. The results showed a high sequence similarity with the mainland Asian tapir group. The comparative sequence analysis of the reserved wild mammals in Thailand and the other endangered mammal species from Southeast Asia comprehensibly verified the potential of our novel primers...
October 19, 2016: Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
https://www.readbyqxmd.com/read/27753072/the-vanishing-black-indian-revisiting-craniometry-and-historic-collections
#18
Pamela L Geller, Christopher M Stojanowksi
OBJECTIVES: This article uses craniometric allocation as a platform for discussing the legacy of Samuel G. Morton's collection of crania, the process of racialization, and the value of contextualized biohistoric research perspectives in biological anthropology. MATERIALS AND METHODS: Standard craniometric measurements were recorded for seven Seminoles in the Samuel G. Morton Crania Collection and 10 European soldiers from the Fort St. Marks Military Cemetery; all individuals were men and died in Florida during the 19th century...
October 18, 2016: American Journal of Physical Anthropology
https://www.readbyqxmd.com/read/27750404/targeted-and-non-targeted-drug-screening-in-whole-blood-by-uhplc-tof-ms-with-data-independent-acquisition
#19
Christian Brinch Mollerup, Petur Weihe Dalsgaard, Marie Mardal, Kristian Linnet
High-resolution mass spectrometry (HRMS) is widely used for the drug screening of biological samples in clinical and forensic laboratories. With the continuous addition of new psychoactive substances (NPS), keeping such methods updated is challenging. HRMS allows for combined targeted and non-targeted screening; first, peaks are identified by software algorithms, and identifications are based on reference standard data. Remaining unknown peaks are attempted identified with in silico and literature data. However, several thousand peaks remain where most are unidentifiable or uninteresting in drug screening...
October 17, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27750077/soil-characterisation-by-bacterial-community-analysis-for-forensic-applications-a-quantitative-comparison-of-environmental-technologies
#20
Habteab Habtom, Sandrine Demanèche, Lorna Dawson, Chen Azulay, Ofra Matan, Patrick Robe, Ron Gafny, Pascal Simonet, Edouard Jurkevitch, Zohar Pasternak
The ubiquity and transferability of soil makes it a resource for the forensic investigator, as it can provide a link between agents and scenes. However, the information contained in soils, such as chemical compounds, physical particles or biological entities, is seldom used in forensic investigations; due mainly to the associated costs, lack of available expertise, and the lack of soil databases. The microbial DNA in soil is relatively easy to access and analyse, having thus the potential to provide a powerful means for discriminating soil samples or linking them to a common origin...
October 6, 2016: Forensic Science International. Genetics
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