keyword
https://read.qxmd.com/read/36358923/proline-rich-region-ii-prr2-plays-an-important-role-in-tau-glycan-interaction-an-nmr-study
#1
JOURNAL ARTICLE
Anqesha Murray, Lufeng Yan, James M Gibson, Jian Liu, David Eliezer, Guy Lippens, Fuming Zhang, Robert J Linhardt, Jing Zhao, Chunyu Wang
(1) Background: Prion-like transcellular spreading of tau pathology in Alzheimer's disease (AD) is mediated by tau binding to the cell-surface glycan heparan sulfate (HS). However, the structural determinants for tau-HS interaction are not well understood. (2) Methods and Results: Binding-site mapping using NMR showed two major binding regions in full-length tau responsible for heparin interaction. Thus, two tau constructs, tau PRR2* and tau R2*, were designed to investigate the molecular details at the tau-heparin binding interface...
October 27, 2022: Biomolecules
https://read.qxmd.com/read/35867417/comparison-table-some-parenteral-anticoagulants-for-vte
#2
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
July 25, 2022: Medical Letter on Drugs and Therapeutics
https://read.qxmd.com/read/35867416/drugs-for-treatment-and-prevention-of-venous-thromboembolism
#3
JOURNAL ARTICLE
(no author information available yet)
No abstract text is available yet for this article.
July 25, 2022: Medical Letter on Drugs and Therapeutics
https://read.qxmd.com/read/35108013/characterization-of-heparin-s-conformational-ensemble-by-molecular-dynamics-simulations-and-nuclear-magnetic-resonance-spectroscopy
#4
JOURNAL ARTICLE
J Joel Janke, Yanlei Yu, Vitor H Pomin, Jing Zhao, Chunyu Wang, Robert J Linhardt, Angel E García
Heparin is a highly charged, polysulfated polysaccharide and serves as an anticoagulant. Heparin binds to multiple proteins throughout the body, suggesting a large range of potential therapeutic applications. Although its function has been characterized in multiple physiological contexts, heparin's solution conformational dynamics and structure-function relationships are not fully understood. Molecular dynamics (MD) simulations facilitate the analysis of a molecule's underlying conformational ensemble, which then provides important information necessary for understanding structure-function relationships...
February 2, 2022: Journal of Chemical Theory and Computation
https://read.qxmd.com/read/34986974/thrombotic-and-hemorrhagic-risk-in-bariatric-surgery-with-multimodal-rehabilitation-programs-comparing-2-reduced-guidelines-for-pharmacological-prophylaxis
#5
JOURNAL ARTICLE
Manuel Gorosabel Calzada, Alberto Hernández Matías, Alejandro Andonaegui de la Madriz, Raquel León Ledesma, Laura Alonso-Lamberti Rizo, Andrea Salazar Carrasco, Juan Carlos Ruiz de Adana, José María Jover Navalón
OBJECTIVE: To determine the thrombotic and hemorrhagic risk in bariatric surgery with multimodal rehabilitation programs, comparing two guidelines of pharmacological prophylaxis recommended in the Guide to the Spanish Society for Obesity Surgery and the Obesity Section of the AEC. METHODS: Cohorts retrospective study from January-2010 to December-2019. Cases of vertical gastrectomy or gastric bypass were recorded, systematically applying multimodal rehabilitation protocols...
January 2022: Cirugia española
https://read.qxmd.com/read/34872655/strategies-in-synthesis-of-heparin-heparan-sulfate-oligosaccharides-2000-present
#6
JOURNAL ARTICLE
Steven B Dulaney, Xuefei Huang
Heparin and heparan sulfate are members of the glycosaminoglycan family that are involved in a multitude of biological processes. The great interests in the anticoagulant properties of heparin have stimulated major advances in synthetic strategies toward clinically effective analogues, as demonstrated importantly by the approval of the fully synthetic pentasaccharide fragment, termed fondaparinux (Arixtra®), of the heparin macromolecule for treatment of deep-vein thrombosis. Given the highly complex nature of heparin and heparan sulfate, the chemical synthesis of their components is a challenging endeavor...
2021: Advances in Carbohydrate Chemistry and Biochemistry
https://read.qxmd.com/read/33454109/thrombotic-and-hemorrhagic-risk-in-bariatric-surgery-with-multimodal-rehabilitation-programs-comparing-2-reduced-guidelines-for-pharmacological-prophylaxis
#7
JOURNAL ARTICLE
Manuel Gorosabel Calzada, Alberto Hernández Matías, Alejandro Andonaegui de la Madriz, Raquel León Ledesma, Laura Alonso-Lamberti Rizo, Andrea Salazar Carrasco, Juan Carlos Ruiz de Adana, José María Jover Navalón
OBJECTIVE: to determine the thrombotic and hemorrhagic risk in bariatric surgery with multimodal rehabilitation programs, comparing 2guidelines of pharmacological prophylaxis recommended in the Guide to the Spanish Society for Obesity Surgery and the Obesity Section of the AEC. METHODS: Cohorts retrospective study from January-2010 to December-2019. Cases of vertical gastrectomy or gastric bypass were recorded, systematically applying multimodal rehabilitation protocols...
January 13, 2021: Cirugia española
https://read.qxmd.com/read/32496799/programmable-one-pot-synthesis-of-heparin-pentasaccharide-fondaparinux
#8
JOURNAL ARTICLE
Supriya Dey, Hong-Jay Lo, Chi-Huey Wong
The clinically approved Fondaparinux (Arixtra) has been used for the treatment of deep vein thrombosis and acute pulmonary embolism since 2002 and is considered to be better than the low-molecular weight heparin in terms of anticoagulation response, duration of action, and biosafety. However, the synthetic methods previously developed for its manufacture are relatively complicated, thus restricting its extensive use. We report here a potentially scalable and programmable one-pot synthesis of Fondaparinux using the [1,2,2] strategy and designed thioglycosides with well-defined reactivity as building blocks...
June 4, 2020: Organic Letters
https://read.qxmd.com/read/32355037/crystal-and-solution-structures-of-fragments-of-the-human-leucocyte-common-antigen-related-protein
#9
JOURNAL ARTICLE
Joachim Vilstrup, Amanda Simonsen, Thea Birkefeldt, Dorthe Strandbygård, Jeppe Lyngsø, Jan Skov Pedersen, Søren Thirup
Leucocyte common antigen-related protein (LAR) is a post-synaptic type I transmembrane receptor protein that is important for neuronal functionality and is genetically coupled to neuronal disorders such as attention deficit hyperactivity disorder (ADHD). To understand the molecular function of LAR, structural and biochemical studies of protein fragments derived from the ectodomain of human LAR have been performed. The crystal structure of a fragment encompassing the first four FNIII domains (LARFN1-4 ) showed a characteristic L shape...
May 1, 2020: Acta Crystallographica. Section D, Structural Biology
https://read.qxmd.com/read/31851608/different-strategies-for-pharmacological-thromboprophylaxis-for-lower-limb-immobilisation-after-injury-systematic-review-and-economic-evaluation
#10
JOURNAL ARTICLE
Abdullah Pandor, Daniel Horner, Sarah Davis, Steve Goodacre, John W Stevens, Mark Clowes, Beverley J Hunt, Tim Nokes, Jonathan Keenan, Kerstin de Wit
BACKGROUND: Thromboprophylaxis can reduce the risk of venous thromboembolism (VTE) during lower-limb immobilisation, but it is unclear whether or not this translates into meaningful health benefit, justifies the risk of bleeding or is cost-effective. Risk assessment models (RAMs) could select higher-risk individuals for thromboprophylaxis. OBJECTIVES: To determine the clinical effectiveness and cost-effectiveness of different strategies for providing thromboprophylaxis to people with lower-limb immobilisation caused by injury and to identify priorities for future research...
December 2019: Health Technology Assessment: HTA
https://read.qxmd.com/read/31705871/evaluating-heparin-products-for-heparin-induced-thrombocytopenia-using-surface-plasmon-resonance
#11
JOURNAL ARTICLE
Fuming Zhang, Payel Datta, Jonathan S Dordick, Robert J Linhardt
Heparin-induced thrombocytopenia (HIT) is an adverse immunological disorder caused by antibodies to platelet factor 4 (PF4)-heparin complexes. The analysis of HIT potential for different heparin and heparin-related products is important prior to their clinical application. Here, we report a rapid method for the evaluation of HIT potential of various heparin and heparin-related compounds using surface plasmon resonance (SPR). Solution competition between surface-immobilized heparin and soluble unfractionated heparin, low molecular weight heparin (LMWH), or ultra-LMWH binding to PF4 was performed using SPR to measure the half maximal inhibitory concentration (IC50 ) of different heparin products...
February 2020: Journal of Pharmaceutical Sciences
https://read.qxmd.com/read/31673318/nanoscale-electrostatic-gating-of-molecular-transport-through-nuclear-pore-complexes-as-probed-by-scanning-electrochemical-microscopy
#12
JOURNAL ARTICLE
Pavithra Pathirathna, Ryan J Balla, Guanqun Meng, Zemeng Wei, Shigeru Amemiya
The nuclear pore complex (NPC) is a large protein nanopore that solely mediates molecular transport between the nucleus and cytoplasm of a eukaryotic cell. There is a long-standing consensus that selective transport barriers of the NPC are exclusively based on hydrophobic repeats of phenylalanine and glycine (FG) of nucleoporins. Herein, we reveal experimentally that charged residues of amino acids intermingled between FG repeats can modulate molecular transport through the NPC electrostatically and in a pathway-dependent manner...
September 14, 2019: Chemical Science
https://read.qxmd.com/read/30916501/-experience-in-diagnostic-assays-for-heparin-induced-thrombocytopenia-experience-of-a-tertiary-hospital-in-israel
#13
JOURNAL ARTICLE
Anat Keren-Politansky, Ron Hoffman, Yona Nadir, Benjamin Brenner, Galit Sarig
AIMS: To analyze the experience of a tertiary medical center in clinical and laboratory diagnosis of suspected HIT. BACKGROUND: The diagnosis of heparin-induced thrombocytopenia (HIT) requires clinical data and laboratory detection of platelet activating factor 4/heparin (PF4/H) antibodies by immunological or functional assays. Although antigen screening assays are widely used, the functional assays are performed only by several expert labs. METHODS: A retrospective review of the Hematology Laboratory database on patients evaluated between the years 2008-2016 at Rambam, identified 412 individuals with clinical suspicion of HIT...
March 2019: Harefuah
https://read.qxmd.com/read/30717501/responsible-safe-and-effective-use-of-antithrombotics-and-anticoagulants-in-patients-undergoing-interventional-techniques-american-society-of-interventional-pain-physicians-asipp-guidelines
#14
JOURNAL ARTICLE
Alan D Kaye, Laxmaiah Manchikanti, Matthew B Novitch, Imran N Mungrue, Muhammad Anwar, Mark R Jones, Erik M Helander, Elyse M Cornett, Matthew R Eng, Jay S Grider, Michael E Harned, Ramsin M Benyamin, John R Swicegood, Thomas T Simopoulos, Salahadin Abdi, Richard D Urman, Timothy R Deer, Cyrus Bakhit, Mahendra Sanapati, Sairam Atluri, Ramarao Pasupuleti, Amol Soin, Sudhir Diwan, Ricardo Vallejo, Kenneth D Candido, Nebojsa Nick Knezevic, Douglas Beall, Sheri L Albers, Richard E Latchaw, Hari Prabhakar, Joshua A Hirsch
BACKGROUND: Interventional pain management involves diagnosis and treatment of chronic pain. This specialty utilizes minimally invasive procedures to target therapeutics to the central nervous system and the spinal column. A subset of patients encountered in interventional pain are medicated using anticoagulant or antithrombotic drugs to mitigate thrombosis risk. Since these drugs target the clotting system, bleeding risk is a consideration accompanying interventional procedures. Importantly, discontinuation of anticoagulant or antithrombotic drugs exposes underlying thrombosis risk, which can lead to significant morbidity and mortality especially in those with coronary artery or cerebrovascular disease...
January 2019: Pain Physician
https://read.qxmd.com/read/28817073/extended-physicochemical-characterization-of-the-synthetic-anticoagulant-pentasaccharide-fondaparinux-sodium-by-quantitative-nmr-and-single-crystal-x-ray-analysis
#15
JOURNAL ARTICLE
William de Wildt, Huub Kooijman, Carel Funke, Bülent Üstün, Afranina Leika, Maarten Lunenburg, Frans Kaspersen, Edwin Kellenbach
Fondaparinux sodium is a synthetic pentasaccharide representing the high affinity antithrombin III binding site in heparin. It is the active pharmaceutical ingredient of the anticoagulant drug Arixtra® . The single crystal X-ray structure of Fondaparinux sodium is reported, unequivocally confirming both structure and absolute configuration. The iduronic acid adopts a somewhat distorted chair conformation. Due to the presence of many sulfur atoms in the highly sulfated pentasaccharide, anomalous dispersion could be applied to determine the absolute configuration...
August 17, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/28589488/negative-electron-transfer-dissociation-sequencing-of-increasingly-sulfated-glycosaminoglycan-oligosaccharides-on-an-orbitrap-mass-spectrometer
#16
JOURNAL ARTICLE
Franklin E Leach, Nicholas M Riley, Michael S Westphall, Joshua J Coon, I Jonathan Amster
The structural characterization of sulfated glycosaminoglycan (GAG) carbohydrates remains an important target for analytical chemists attributable to challenges introduced by the natural complexity of these mixtures and the defined need for molecular-level details to elucidate biological structure-function relationships. Tandem mass spectrometry has proven to be the most powerful technique for this purpose. Previously, electron detachment dissociation (EDD), in comparison to other methods of ion activation, has been shown to provide the largest number of useful cleavages for de novo sequencing of GAG oligosaccharides, but such experiments are restricted to Fourier transform ion cyclotron resonance mass spectrometers (FTICR-MS)...
September 2017: Journal of the American Society for Mass Spectrometry
https://read.qxmd.com/read/26216842/the-binding-of-pentapeptides-to-biological-and-synthetic-high-affinity-heparin
#17
JOURNAL ARTICLE
Ragnar Flengsrud, Simen Gjelseth Antonsen
Pentapeptides have been shown to bind the synthetic heparin fondaparinux (Arixtra) as well the biological heparins dalteparin (Fragmin) and salmon heparin. In contrast to heparin binding consensus sequences, the pentapeptides are acidic or neutral, with no arginine or histidine residue. The peptides showed an effect on in vitro heparin anti-factor X activity with a reduction of fondaparinux activity by 65-95%. Heparin binding was further studied by using peptide solid phase chromatography and NMR analysis.
November 1, 2015: Bioorganic & Medicinal Chemistry Letters
https://read.qxmd.com/read/26205797/-prevention-of-venous-thromboembolic-events-by-fondaparinux-2-5-mg-in-general-practice-archimed-ville
#18
MULTICENTER STUDY
I Mahé, J-P Daurès, D Pouchain, I Quéré, C Aubin, J Doussaint, S Schück, C Leroyer
OBJECTIVE: To evaluate the mean duration of treatment course with fondaparinux 2.5 mg (ARIXTRA(®)) in the setting of ambulatory general medicine, with respect to its indication in thromboprophylaxis for medically ill patients and to describe the population treated. METHODS: Observational, prospective, national, multicenter, pharmaco-epidemiological study, performed in France, at the request of the Transparency Commission (a division of the French Health Regulatory Authority)...
December 2015: Journal des Maladies Vasculaires
https://read.qxmd.com/read/26115461/investigating-changes-in-the-gas-phase-conformation-of-antithrombin-iii-upon-binding-of-arixtra-using-traveling-wave-ion-mobility-spectrometry-twims
#19
JOURNAL ARTICLE
Yuejie Zhao, Arunima Singh, Lingyun Li, Robert J Linhardt, Yongmei Xu, Jian Liu, Robert J Woods, I Jonathan Amster
We validate the utility of ion mobility to measure protein conformational changes induced by the binding of glycosaminoglycan ligands, using the well characterized system of Antithrombin III (ATIII) and Arixtra, a pharmaceutical agent with heparin (Hp) activity. Heparin has been used as a therapeutic anticoagulant drug for several decades through its interaction with ATIII, a serine protease inhibitor that plays a central role in the blood coagulation cascade. This interaction induces conformational changes within ATIII that dramatically enhance the ATIII-mediated inhibition rate...
October 21, 2015: Analyst
https://read.qxmd.com/read/24970196/structural-evidence-for-the-tetrameric-assembly-of-chemokine-ccl11-and-the-glycosaminoglycan-arixtra%C3%A2
#20
JOURNAL ARTICLE
Andrew B Dykstra, Matt D Sweeney, Julie A Leary
Understanding chemokine interactions with glycosaminoglycans (GAG) is critical as these interactions have been linked to a number of inflammatory medical conditions, such as arthritis and asthma. To better characterize in vivo protein function, comprehensive knowledge of multimeric species, formed by chemokines under native conditions, is necessary. Herein is the first report of a tetrameric assembly of the human chemokine CCL11, which was shown bound to the GAG Arixtra™. Isothermal titration calorimetry data indicated that CCL11 interacts with Arixtra, and ion mobility mass spectrometry (IM-MS) was used to identify ions corresponding to the CCL11 tetrameric species bound to Arixtra...
November 6, 2013: Biomolecules
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