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steroid profiling mass spectrometry

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https://www.readbyqxmd.com/read/28533544/sex-specific-metabolic-profiles-of-androgens-and-its-main-binding-protein-shbg-in-a-middle-aged-population-without-diabetes
#1
Uwe Piontek, Henri Wallaschofski, Gabi Kastenmüller, Karsten Suhre, Henry Völzke, Kieu Trinh Do, Anna Artati, Matthias Nauck, Jerzy Adamski, Nele Friedrich, Maik Pietzner
The role of androgens in metabolism with respect to sex-specific disease associations is poorly understood. Therefore, we aimed to provide molecular signatures in plasma and urine of androgen action in a sex-specific manner using state-of-the-art metabolomics techniques. Our study population consisted of 430 men and 343 women, aged 20-80 years, who were recruited for the cross-sectional population-based Study of Health in Pomerania (SHIP-TREND), Germany. We used linear regression models to identify associations between testosterone, androstenedione and dehydroepiandrosterone-sulfate (DHEAS) as well as sex hormone-binding globulin and plasma or urine metabolites measured by mass spectrometry...
May 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28527414/structural-characterization-and-discrimination-of-the-paris-polyphylla-var-yunnanensis-and-paris-vietnamensis-based-on-metabolite-profiling-analysis
#2
Li-Ping Kang, Yuan-Yuan Huang, Zhi-Lai Zhan, Da-Hui Liu, Hua-Sheng Peng, Tie-Gui Nan, Yuan Zhang, Qing-Xiu Hao, Jin-Fu Tang, Shou-Dong Zhu, Guang Yang, Lan-Ping Guo, Min Chen, Lu-Qi Huang
This study aimed to distinguish the rhizomes of Paris polyphylla var. yunnanensis (Franch) Hand Mazz (PPY) and Paris veitnamensis (Takht.) H. Li (PV) using metabolomics-based ultra high-performance liquid chromatography coupled with quadrupole time-of-fligh mass spectrometry (UHPLC/Q-TOF MS). First, the UHPLC/Q-TOF MS approach was optimized for metabolite profiling. Then, the MS data were processed using UNIFI™ combined with an in-house library to automatically characterize the metabolites. Based on the exact mass information, the fragmentation characteristics, and the retention time of compounds, and the fragmentation mechanism and retention behavior of steroidal glycosides in the references, the structures identified by UNIFI were further verified...
May 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28521850/differential-gene-expression-in-porcine-oviduct-during-the-oestrous-cycle
#3
O S Acuña, M Avilés, R López-Úbeda, A Guillén-Martínez, C Soriano-Úbeda, A Torrecillas, P Coy, M J Izquierdo-Rico
The oviduct undergoes changes under the influence of steroid hormones during the oestrous cycle. However, the molecular mechanisms underlying oviductal regulation are not fully understood. The aim of the present study was to identify the gene expression profile of the porcine oviduct in different stages of the cycle using microarray technology. A systematic study was performed on animals at four different stage: prepubertal gilts, and sows in the preovulatory, postovulatory and luteal phase of the oestrous cycle...
May 19, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28501193/immunoaffinity-chromatography-combined-with-tandem-mass-spectrometry-a-new-tool-for-the-selective-capture-and-analysis-of-brassinosteroid-plant-hormones
#4
Jana Oklestkova, Danuše Tarkowská, Luděk Eyer, Tomáš Elbert, Aleš Marek, Zora Smržová, Ondřej Novák, Milan Fránek, Vladimir N Zhabinskii, Miroslav Strnad
Brassinosteroids (BRs) are plant-specific steroid hormones that play essential roles in the regulation of many important physiological processes in plant life. Their extremely low concentrations (~pmoles/g FW) in plant tissue and huge differences in polarity of individual members within the BR family hamper their detection and quantification. To address this problem, an immunoaffinity sorbent with broad specificity and high capacity for different BR metabolites containing a monoclonal antibody (mAb) against a BR spacer (20S)-2α,3α-dihydroxy-7-oxa-7α-homo-5α-pregnane-6-one-20 carboxylic acid (BR4812) was used for the rapid and highly selective isolation of endogenous BRs containing a 2α,3α-diol in ring A from minute plant samples...
August 1, 2017: Talanta
https://www.readbyqxmd.com/read/28467654/sterol-regulatory-element-binding-protein-1c-knockout-mice-show-altered-neuroactive-steroid-levels-in-sciatic-nerve
#5
Nico Mitro, Gaia Cermenati, Matteo Audano, Silvia Giatti, Marzia Pesaresi, Silvia Pedretti, Roberto Spezzano, Donatella Caruso, Roberto Cosimo Melcangi
Neuroactive steroid levels are altered in several experimental models of peripheral neuropathy, and on this basis, they have been proposed as protective agents. For the first time, the levels of these molecules were here assessed in sterol regulatory-binding protein-1c (SREBP-1c) knock-out (KO) male mice (i.e., an experimental model of peripheral neuropathy) and compared with observations in wild type animals. The levels of neuroactive steroids have been evaluated by liquid chromatography tandem mass spectrometry in plasma and sciatic nerve at two and ten months of age and these analyses were implemented analyzing the gene expression of crucial steroidogenic enzymes in sciatic nerve...
May 3, 2017: Journal of Neurochemistry
https://www.readbyqxmd.com/read/28396286/altered-mitochondrial-and-peroxisomal-integrity-in-lipocalin-2-deficient-mice-with-hepatic-steatosis
#6
Anastasia Asimakopoulou, Annabelle Fülöp, Erawan Borkham-Kamphorst, Eddy Van de Leur, Nikolaus Gassler, Thorsten Berger, Birte Beine, Helmut E Meyer, Tak W Mak, Carsten Hopf, Corinna Henkel, Ralf Weiskirchen
Lipocalin-2 (LCN2) is a secreted adipokine that transports small hydrophobic molecules such as fatty acids and steroids. LCN2 limits bacterial growth by sequestering iron-containing siderophores and in mammalian liver protects against inflammation, infection, injury and other stressors. Because LCN2 modulates hepatic fat metabolism and homeostasis, we performed a comparative profiling of proteins and lipids of wild type (WT) and Lcn2-deficient mice fed either standard chow or a methionine- and choline-deficient (MCD) diet...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28393582/insights-into-the-human-brain-proteome-disclosing-the-biological-meaning-of-protein-networks-in-cerebrospinal-fluid
#7
Paulo Bastos, Rita Ferreira, Bruno Manadas, Paula I Moreira, Rui Vitorino
Cerebrospinal fluid (CSF) is an excellent source of biological information regarding the nervous system, once it is in close contact and accurately reflects alterations in this system. Several studies have analyzed differential protein profiles of CSF samples between healthy and diseased human subjects. However, the pathophysiological mechanisms and how CSF proteins relate to diseases are still poorly known. By applying bioinformatics tools, we attempted to provide new insights on the biological and functional meaning of proteomics data envisioning the identification of putative disease biomarkers...
May 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/28382793/rna-sample-preparation-applied-to-gene-expression-profiling-for-the-horse-biological-passport
#8
Ludovic Bailly-Chouriberry, Florent Baudoin, Florence Cormant, Yohan Glavieux, Benoit Loup, Patrice Garcia, Marie-Agnès Popot, Yves Bonnaire
The improvement of doping control is an on-going race. Techniques to fight against doping are usually based on the direct detection of drugs or their metabolites by analytical methods such as chromatography hyphenated to mass spectrometry after ad hoc sample preparation. Nowadays, omic methods constitute an attractive development and advances have been achieved particularly by application of molecular biology tools for detection of anabolic androgenic steroids (AAS), erythropoiesis-stimulating agent (ESA) or to control human growth hormone misuses...
April 5, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28356371/metabolomics-analysis-identifies-different-metabotypes-of-asthma-severity
#9
Stacey N Reinke, Héctor Gallart-Ayala, Cristina Gómez, Antonio Checa, Alexander Fauland, Shama Naz, Muhammad Anas Kamleh, Ratko Djukanović, Timothy S C Hinks, Craig E Wheelock
In this study, we sought to determine whether asthma has a metabolic profile and whether this profile is related to disease severity.We characterised the serum from 22 healthy individuals and 54 asthmatics (12 mild, 20 moderate, 22 severe) using liquid chromatography-high-resolution mass spectrometry-based metabolomics. Selected metabolites were confirmed by targeted mass spectrometry assays of eicosanoids, sphingolipids and free fatty acids.We conclusively identified 66 metabolites; 15 were significantly altered with asthma (p≤0...
March 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28351628/coupling-of-gas-chromatography-and-electrospray-ionization-high-resolution-mass-spectrometry-for-the-analysis-of-anabolic-steroids-as-trimethylsilyl-derivatives-in-human-urine
#10
Eunju Cha, Eun Sook Jeong, Sangwon Cha, Jaeick Lee
In this study, gas chromatography (GC) was interfaced with high resolution mass spectrometry (HRMS) with electrospray ionization source (ESI) and the relevant parameters were investigated to enhance the ionization efficiency. In GC-ESI, the distances (x-, y- and z) and angle between the ESI needle, GC capillary column and MS orifice were set to 7 (x-distance), 4 (y-distance), and 1 mm (z-distance). The ESI spray solvent, acid modifier and nebulizer gas flow were methanol, 0.1% formic acid and 5 arbitrary units, respectively...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28351627/non-targeted-lc-ms-based-metabolomics-analysis-of-the-urinary-steroidal-profile
#11
Amelia Palermo, Francesco Botrè, Xavier de la Torre, Nicola Zamboni
The urinary steroidal fraction has been extensively explored as non-invasive alternative to monitor pathological conditions as well as to unveil the illicit intake of pseudo-endogenous anabolic steroids in sport. However, the majority of previous approaches involved the a priori selection of potentially relevant target analytes. Here we describe the non-targeted analysis of the urinary steroidal profiles. The workflow includes minimal sample pretreatment and normalization according to the specific gravity of urine, a 20 min reverse phase ultra-performance liquid chromatographic separation hyphenated to electrospray time-of-flight mass spectrometry...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28326509/the-steroid-response-to-human-chorionic-gonadotropin-hcg-stimulation-in-men-with-klinefelter-syndrome-does-not-change-using-immunoassay-or-mass-spectrometry
#12
L Roli, D Santi, S Belli, S Tagliavini, S Cavalieri, M C De Santis, E Baraldi, F Fanelli, M Mezzullo, A R Granata, U Pagotto, R Pasquali, V Rochira, C Carani, M Simoni, T Trenti
PURPOSE: Liquid-chromatography tandem mass-spectrometry (LC-MS/MS) was developed in parallel to Immunoassays (IAs) and today is proposed as the "gold standard" for steroid assays. Leydig cells of men with Klinefelter syndrome (KS) are able to respond to human chorionic gonadotropin (hCG) stimulation, even if testosterone (T) production was impaired. The aim was to evaluate how results obtained by IAs and LC-MS/MS can differently impact on the outcome of a clinical research on gonadal steroidogenesis after hCG stimulation...
March 21, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28259259/endometriosis-is-associated-with-aberrant-metabolite-profiles-in-plasma
#13
Sophia Letsiou, Dirkje P Peterse, Amelie Fassbender, Margriet M Hendriks, Niels J van den Broek, Rudolf Berger, Dorien F O, Arne Vanhie, Alexandra Vodolazkaia, Anne Van Langendonckt, Jacques Donnez, Amy C Harms, Rob J Vreeken, Patrick G Groothuis, Marie-Madeleine Dolmans, Arjan B Brenkman, Thomas M D'Hooghe
OBJECTIVE: To identify metabolites that are associated with and predict the presence of endometriosis. DESIGN: Metabolomics study using state-of-the-art mass spectrometry approaches. SETTING: University hospital and universities. PATIENT(S): Twenty-five women with laparoscopically confirmed endometriosis (cases) and 19 women with laparoscopically documented absence of endometriosis (controls). None of the women included in this study had received oral contraception or GnRH agonists for a minimum of 1 month before blood collection...
March 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28249803/venomics-analyses-of-the-skin-secretion-of-dermatonotus-muelleri-preliminary-proteomic-and-metabolomic-profiling
#14
Ingrid Duarte Cavalcante, Marta Maria Antoniazzi, Carlos Jared, Osmindo R Pires, Juliana Mozer Sciani, Daniel Carvalho Pimenta
Dermatonotus muelleri is the sole species of the Dermatonotus genus and inhabits Argentina, Bolivia, Brazil and Paraguay. This animal exhibits an explosive reproductive behavior during the Southern spring months, which lasts only for five days. Moreover, this animal displays specific adaptations to the habitat resulting in the energy conservation needed during either the intense reproduction period or times of estivation. During dry seasons and/or food shortages D. muelleri can survive because its food specialization and ability to dig an underground chamber for protection...
February 27, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28235592/steroid-profiling-in-h295r-cells-to-identify-chemicals-potentially-disrupting-the-production-of-adrenal-steroids
#15
Petra Strajhar, David Tonoli, Fabienne Jeanneret, Raphaella M Imhof, Vanessa Malagnino, Melanie Patt, Denise V Kratschmar, Julien Boccard, Serge Rudaz, Alex Odermatt
The validated OECD test guideline 456 based on human adrenal H295R cells promotes measurement of testosterone and estradiol production as read-out to identify potential endocrine disrupting chemicals. This study aimed to establish optimal conditions for using H295R cells to detect chemicals interfering with the production of key adrenal steroids. H295R cells' supernatants were characterized by liquid chromatography-mass spectrometry (LC-MS)-based steroid profiling, and the influence of experimental conditions including time and serum content was assessed...
April 15, 2017: Toxicology
https://www.readbyqxmd.com/read/28222414/urinary-steroid-metabolites-in-a-case-of-florid-ectopic-cushing-s-syndrome-and-clinical-correlations
#16
Angelos Kyriacou, Karolina M Stepien, Basil Issa
A 51-year old woman was admitted with multiple cerebral, pulmonary and intra-abdominal abscesses. The combination of apparent immunosuppression, obesity, diabetes mellitus, hypertension, hypokalaemia, osteoporotic fractures and bilateral shoulder avascular necrosis led to a clinical diagnosis of Cushing's syndrome (CS). This was biochemically confirmed as follows: midnight serum cortisol 4275 nmol/L (60-250), non-suppressed overnight dexamethasone suppression test, raised salivary cortisol 716 nmol/L (5-46) and ACTH 639 ng/L (0-46)...
October 2016: Hormones: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28146078/metabolomic-biomarkers-in-urine-of-cushing-s-syndrome-patients
#17
Alicja Kotłowska, Tomasz Puzyn, Krzysztof Sworczak, Piotr Stepnowski, Piotr Szefer
Cushing's syndrome (CS) is a disease which results from excessive levels of cortisol in the human body. The disorder is associated with various signs and symptoms which are also common for the general population not suffering from compound hypersecretion. Thus, more sensitive and selective methods are required for the diagnosis of CS. This follow-up study was conducted to determine which steroid metabolites could serve as potential indicators of CS and possible subclinical hypercortisolism in patients diagnosed with so called non-functioning adrenal incidentalomas (AIs)...
January 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28105335/detection-of-stanozolol-in-the-urine-of-athletes-at-a-pg-level-the-possibility-of-passive-exposure
#18
Christina Tsitsimpikou, Konstantinos Tsarouhas, Demetrios A Spandidos, Aristides M Tsatsakis
Stanozolol is a synthetic heterocyclic steroid with anabolic and androgenic properties, which has been abused by several high-profile professional athletes. Stanozolol is also used in veterinary medicine to increase appetite, cause weight gain and treat certain types of anemia. The detection of stanozolol metabolites in human urine for doping control purposes depends on the analytical method applied. The most commonly applied methods in the World Anti-Doping Agency (WADA)-accredited doping control laboratories are gas chromatography/high-resolution mass spectrometry (GC/HRMS) or gas chromatography-mass spectrometry (GC/MS/MS)...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28076306/parallel-diurnal-fluctuation-of-testosterone-androstenedione-dehydroepiandrosterone-and-17ohprogesterone-as-assessed-in-serum-and-saliva-validation-of-a-novel-liquid-chromatography-tandem-mass-spectrometry-method-for-salivary-steroid-profiling
#19
Marco Mezzullo, Alessia Fazzini, Alessandra Gambineri, Guido Di Dalmazi, Roberta Mazza, Carla Pelusi, Valentina Vicennati, Renato Pasquali, Uberto Pagotto, Flaminia Fanelli
BACKGROUND: Salivary androgen testing represents a valuable source of biological information. However, the proper measurement of such low levels is challenging for direct immunoassays, lacking adequate accuracy. In the last few years, many conflicting findings reporting low correlation with the serum counterparts have hampered the clinical application of salivary androgen testing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) makes it possible to overcome previous analytical limits, providing new insights in endocrinology practice...
January 11, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28074002/metabolic-profiling-in-formalin-fixed-and-paraffin-embedded-prostate-cancer-tissues
#20
Stefano Cacciatore, Giorgia Zadra, Clyde Bango, Kathryn L Penney, Svitlana Tyekucheva, Oscar Yanes, Massimo Loda
Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively...
April 2017: Molecular Cancer Research: MCR
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