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steroid profiling mass spectrometry

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https://www.readbyqxmd.com/read/27881557/plasma-metabolomics-implicate-modified-transfer-rnas-and-altered-bioenergetics-in-the-outcome-of-pulmonary-arterial-hypertension
#1
Christopher J Rhodes, Pavandeep Ghataorhe, John Wharton, Kevin C Rue-Albrecht, Charaka Hadinnapola, Geoffrey Watson, Marta Bleda, Matthias Haimel, Gerry Coghlan, Paul A Corris, Luke S Howard, David G Kiely, Andrew J Peacock, Joanna Pepke-Zaba, Mark Toshner, Stephen J Wort, J Simon R Gibbs, Allan Lawrie, Stefan Gräf, Nicholas W Morrell, Martin R Wilkins
BACKGROUND: -Pulmonary arterial hypertension (PAH) is a heterogeneous disorder with high mortality. METHODS: -We conducted a comprehensive study of plasma metabolites using ultra-performance liquid chromatography mass-spectrometry to (1) identify patients at high risk of early death, (2) identify patients who respond well to treatment and (3) provide novel molecular insights into disease pathogenesis. RESULTS: -53 circulating metabolites distinguished well-phenotyped patients with idiopathic or heritable PAH (n=365) from healthy controls (n=121) following correction for multiple testing (p<7...
November 21, 2016: Circulation
https://www.readbyqxmd.com/read/27845856/modified-release-and-conventional-glucocorticoids-and-diurnal-androgen-excretion-in-congenital-adrenal-hyperplasia
#2
Christopher M Jones, Ashwini Mallappa, Nicole Reisch, Nikolaos Nikolaou, Nils Krone, Beverly A Hughes, Donna M O'Neil, Martin J Whitaker, Jeremy W Tomlinson, Karl-Heinz Storbeck, Deborah P Merke, Richard J Ross, Wiebke Arlt
CONTEXT: The classic androgen synthesis pathway proceeds via DHEA, androstenedione and testosterone to 5α-dihydrotestosterone (DHT). However, DHT synthesis can also be achieved by an alternative pathway originating from 17α-hydroxyprogesterone (17OHP), which accumulates in congenital adrenal hyperplasia (CAH). Similarly, recent work has highlighted androstenedione-derived 11-oxygenated 19-carbon steroids as active androgens and, in CAH, androstenedione is generated directly from 17OHP...
November 15, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27842983/a-novel-hplc-mrm-strategy-to-discover-unknown-and-long-term-metabolites-of-stanozolol-for-expanding-analytical-possibilities-in-doping-control
#3
Zhe Wang, Xinmiao Zhou, Xin Liu, Ying Dong, Jinlan Zhang
Stanozolol is one of the most commonly abused anabolic androgenic steroids (AAS) by athletes and usually detected by its parent drug and major metabolites. However, its metabolic pathway is complex, varied and individually different, it is important to characterize its overall metabolic profiles and discover new and long-term metabolites for the aims of expanding detection windows. High performance liquid chromatography coupled with triple quadrupole mass spectrometer (HPLC-MS/MS) was used to analyze the human urine after oral administration of stanozolol...
November 8, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27827563/analytical-strategies-to-detect-enobosarm-administration-in-bovines
#4
Nora Cesbron, Alexandre Sydor, Mylène Penot, Stéphanie Prevost, Bruno Le Bizec, Gaud Dervilly-Pinel
Selective androgen receptor modulators (SARMs) are a novel class of androgen receptor ligands. They are intended to exhibit the same kind of effects as androgenic drugs, like anabolic steroids, but be much more selective in their action, targeting particular tissues without any undesirable effects on others. While the main applications of these synthetic substances are for therapeutic purposes, they also have a high potential for misuse in veterinary practice and the sporting world. In order to guarantee the consumers with food from animal origin free from any residues of such compounds, analytical strategies are required to ensure safe food and also enable fair trade between producers...
November 9, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27770750/oxidative-defense-metabolites-induced-by-salinity-stress-in-roots-of-salicornia-herbacea
#5
Seung Jae Lee, Eun-Mi Jeong, Ah Young Ki, Kyung-Seo Oh, Joseph Kwon, Jae-Hyuk Jeong, Nam-Jin Chung
High salinity is a major abiotic stress that affects the growth and development of plants. This type of stress can influence flowering, the production of crops, defense mechanisms and other physiological processes. Previous studies have attempted to elucidate salt-tolerance mechanisms to improve plant growth and productivity in the presence of sodium chloride. One such plant that has been studied in detail is Salicornia, a well-known halophyte, which has adapted to grow in the presence of high salt. To further the understanding of how Salicornia grows and develops under high saline conditions, Salicornia herbacea (S...
September 29, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27750143/exploring-the-neural-mechanisms-of-finasteride-a-proteomic-analysis-in-the-nucleus-accumbens
#6
Alessio Soggiu, Cristian Piras, Viviana Greco, Paola Devoto, Andrea Urbani, Luigino Calzetta, Marco Bortolato, Paola Roncada
The enzyme 5α-reductase (5αR) catalyzes the conversion of progesterone and testosterone into neuroactive steroids implicated in a wide array of behavioral functions. The prototypical 5αR inhibitor, finasteride (FIN), is clinically approved for the treatment of androgenic alopecia and benign prostatic hyperplasia. Recent evidence has shown that FIN, albeit generally well tolerated, can induce untoward psychological effects in a subset of patients; furthermore, this drug may have therapeutic efficacy for a number of different neuropsychiatric conditions, ranging from Tourette syndrome to schizophrenia...
October 6, 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/27717154/application-of-testosterone-to-epitestosterone-ratio-to-horse-urine-a-complimentary-approach-to-detect-the-administrations-of-testosterone-and-its-pro-drugs-in-thoroughbred-geldings
#7
Marjaana Viljanto, James Scarth, Pamela Hincks, Lynn Hillyer, Adam Cawley, Craig Suann, Glenys Noble, Christopher J Walker, Andrew T Kicman, Mark C Parkin
Detection of testosterone and/or its pro-drugs in the gelding is currently regulated by the application of an international threshold for urinary testosterone of 20 ng/mL. The use of steroid ratios may provide a useful supplementary approach to aid in differentiating between the administration of these steroids and unusual physiological conditions that may result in atypically high testosterone concentrations. In the current study, an ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed to quantify testosterone (T) and epitestosterone (E)...
October 7, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27714988/intranasal-delivery-of-natesto%C3%A2-testosterone-gel-and-its-effects-on-doping-markers
#8
Geoffrey D Miller, Vinod Nair, M Scott Morrison, Maggie Summers, Stuart E Willick, Daniel Eichner
The laboratory profile of intranasal testosterone gel has not been previously reported from an anti-doping perspective. Because intranasal testosterone gel is newly available as a commercial product, we sought to examine the laboratory parameters following administration of this formulation, with particular attention to anti-doping guidelines. Five healthy and active male subjects were administered testosterone intranasal gel three times daily for four weeks, using a five consecutive days on, two days off, pattern...
October 7, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27669235/new-potential-biomarker-for-methasterone-misuse-in-human-urine-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#9
Jianli Zhang, Jianghai Lu, Yun Wu, Xiaobing Wang, Youxuan Xu, Yinong Zhang, Yan Wang
In this study, methasterone urinary metabolic profiles were investigated by liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS) in full scan and targeted MS/MS modes with accurate mass measurement. A healthy male volunteer was asked to take the drug and liquid-liquid extraction was employed to process urine samples. Chromatographic peaks for potential metabolites were hunted out with the theoretical [M - H](-) as a target ion in a full scan experiment and actual deprotonated ions were studied in targeted MS/MS experiment...
2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27649607/chemical-composition-of-ethanolic-extracts-of-some-wild-mushrooms-from-tanzania-and-their-medicinal-potentials
#10
Baraka Luca Chelela, Musa Chacha, Athanasia Matemu
The ethanolic extracts of 5 edible and inedible wild mushrooms collected from the Southern Highlands of Tanzania were characterized by gas chromatography-mass spectrometry. A total of 75 chemical compounds were obtained, mainly fatty acids, carotenoids, alkaloids, phenols, terpernes, steroids, pyranoside, saccharides, and amino acids. Chemical compounds were identified from the ethanolic extract of Russula cellulata, R. kivuensis, Lactarius densifolius, L. gymnocarpoides, and Lactarius sp. In addition, mass spectra of 4 major groups of compounds were also determined...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27648966/body-fat-mass-is-associated-with-ratio-of-steroid-metabolites-reflecting-17-20-lyase-activity-in-prepubertal-girls
#11
Shin-Hye Kim, Ju-Yeon Moon, Hironobu Sasano, Man Ho Choi, Mi-Jung Park
CONTEXT: Pediatric obesity has been related to hyperandrogenism and premature adrenarche in previous studies. However, little is known regarding the association between body fat mass and steroidogenic enzyme activities in children. OBJECTIVE: To examine whether body fat mass is associated with serum steroid profiles in girls. DESIGN, PARTICIPANTS, AND SETTING: We enrolled 242 girls (125 prepubertal, 117 pubertal; aged 7-13 years). Early morning blood samples were drawn at a university hospital to measure serum steroid profiles using gas chromatography-mass spectrometry, and steroidogenic enzyme activities were assessed from the ratios of steroid metabolites...
September 20, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27582398/eicosanoid-mediators-in-the-airway-inflammation-of-asthmatic-patients-what-is-new
#12
REVIEW
Marek Sanak
Lipid mediators contribute to inflammation providing both pro-inflammatory signals and terminating the inflammatory process by activation of macrophages. Among the most significant biologically lipid mediators, these are produced by free-radical or enzymatic oxygenation of arachidonic acid named "eicosanoids". There were some novel eicosanoids identified within the last decade, and many of them are measurable in clinical samples by affordable chromatography-mass spectrometry equipment or sensitive immunoassays...
November 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27571716/effects-of-azocyclotin-on-gene-transcription-and-steroid-metabolome-of-hypothalamic-pituitary-gonad-axis-and-their-consequences-on-reproduction-in-zebrafish-danio-rerio
#13
You-Ning Ma, Chu-Yan Cao, Qiang-Wei Wang, Wen-Jun Gui, Guo-Nian Zhu
The widely used organotins have the potential to disrupt the endocrine system, but little is known of underlying mechanisms of azocyclotin toxicity in fish. The objective of the present study was to investigate the impact of azocyclotin on reproduction in zebrafish. Adult zebrafish were exposed to 0.09 and 0.45μg/L azocyclotin for 21days, and effects on steroid hormones and mRNA expression of the genes belonging to the hypothalamic-pituitary-gonad (HPG) axis were investigated. Mass spectrometry methodology was developed to profile steroids within the metabolome of the gonads...
October 2016: Aquatic Toxicology
https://www.readbyqxmd.com/read/27571131/steroid-profiling-in-male-wobbler-mouse-a-model-of-amyotrophic-lateral-sclerosis
#14
Maria Claudia Gonzalez Deniselle, Philippe Liere, Antoine Pianos, Maria Meyer, Fanny Aprahamian, Annie Cambourg, Noelia P Di Giorgio, Michael Schumacher, Alejandro F De Nicola, Rachida Guennoun
The The Wobbler mouse is an animal model for human motoneuron diseases, especially amyotrophic lateral sclerosis (ALS), used in the investigation of both pathology and therapeutic treatment. ALS is a fatal neurodegenerative disease, characterized by the selective and progressive death of motoneurons, leading to progressive paralysis. Previous limited studies have reported steroidal hormone dysregulation in Wobbler mouse and in ALS patients, suggesting endocrine dysfunctions which may be involved in the pathogenesis of the disease...
August 29, 2016: Endocrinology
https://www.readbyqxmd.com/read/27544322/the-urinary-steroidome-of-treated-children-with-classic-21-hydroxylase-deficiency
#15
Clemens Kamrath, Lisa Wettstaedt, Claudia Boettcher, Michaela F Hartmann, Stefan A Wudy
Monitoring treatment of children with classic congenital adrenal hyperplasia (CAH) is difficult and biochemical targets are not well defined. We retrospectively analysed 576 daily urinary steroid hormone metabolite profiles determined by gas chromatography-mass spectrometry of 150 children aged 3.0-17.9 years with classic 21-hydroxylase deficiency (21-OHD) on hydrocortisone and fludrocortisone treatment. Daily urinary excretion of glucocorticoid-, 17α-hydroxyprogesterone (17-OHP)-, and androgen metabolites as well as growth and weight gain are presented...
January 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27531846/simultaneous-quantitation-of-nine-hydroxy-androgens-and-their-conjugates-in-human-serum-by-stable-isotope-dilution-liquid-chromatography-electrospray-ionization-tandem-mass-spectrometry
#16
Tianzhu Zang, Daniel Tamae, Clementina Mesaros, Qingqing Wang, Meng Huang, Ian A Blair, Trevor M Penning
Castration resistant prostate cancer (CRPC), the fatal form of prostate cancer, remains androgen dependent despite castrate levels of circulating testosterone (T) and 5α-dihydrotestosterone (DHT). To investigate mechanisms by which the tumor can synthesize its own androgens and develop resistance to abiraterone acetate and enzalutamide, methods to measure a complete androgen profile are imperative. Here, we report the development and validation of a stable isotope dilution liquid chromatography electrospray ionization tandem mass spectrometric (SID-LC-ESI-MS/MS) method to quantify nine human hydroxy-androgens as picolinates, simultaneously with requisite specificity and sensitivity...
January 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/27489027/ultraperformance-liquid-chromatography-tandem-mass-spectrometry-method-for-profiling-ketolic-and-phenolic-sex-steroids-using-an-automated-injection-program-combined-with-diverter-valve-switch-and-step-analysis
#17
Caihong Wang, Caisheng Wu, Lin Zhang, Jinlan Zhang
Sex steroids are involved in many physiological and pathological processes. The determination of sex steroids is essential to understand the mechanisms of human health and diseases. Derivatization techniques could specifically enhance the sensitivities for sex steroids with a given functional group. However, no derivatization reagents are available for profiling multifunctional sex steroids, including phenolic estrogens, ketolic androgens, and ketolic progestogens, in a single analytical run. In the present study, a novel method involving ultraperformance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-MS/MS) was developed for profiling both ketolic and phenolic sex steroids in human serum using an automated injection program combined with diverter valve switch and step analysis (AIDSA)...
August 16, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27434237/metabolic-profiles-of-obesity-in-american-indians-the-strong-heart-family-study
#18
Qi Zhao, Yun Zhu, Lyle G Best, Jason G Umans, Karan Uppal, ViLinh T Tran, Dean P Jones, Elisa T Lee, Barbara V Howard, Jinying Zhao
Obesity is a typical metabolic disorder resulting from the imbalance between energy intake and expenditure. American Indians suffer disproportionately high rates of obesity and diabetes. The goal of this study is to identify metabolic profiles of obesity in 431 normoglycemic American Indians participating in the Strong Heart Family Study. Using an untargeted liquid chromatography-mass spectrometry, we detected 1,364 distinct m/z features matched to known compounds in the current metabolomics databases. We conducted multivariate analysis to identify metabolic profiles for obesity, adjusting for standard obesity indicators...
2016: PloS One
https://www.readbyqxmd.com/read/27394176/metabolomic-profile-for-the-early-detection-of-coronary-artery-disease-by-using-uplc-qtof-ms
#19
Xiaobao Xu, Beibei Gao, Qijie Guan, Dandan Zhang, Xianhua Ye, Liang Zhou, Guoxin Tong, Hong Li, Lin Zhang, Jingkui Tian, Jinyu Huang
Traditional risk factors cannot promote prediction capacity for the patients with coronary artery disease (CAD), who usually do not show apparent symptoms until they develop acute myocardial infarction (AMI). As such, novel predictive diagnostic strategies are essential to accurately define patients at risk of acute coronary syndrome. In this study, non-targeted metabolomic profiling using ultra-performance liquid chromatography coupled to time of flight mass spectrometry (UPLC-QTOF/MS) was performed in combination with multivariate statistical model to analyze the serum samples of patients with stable angina (n=38), acute myocardial infarction (AMI) (n=34) and healthy age- and gender-matched controls (n=71)...
September 10, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27374292/gender-and-age-associated-differences-in-serum-metabolite-profiles-among-japanese-populations
#20
Kosuke Saito, Keiko Maekawa, Jason M Kinchen, Rieko Tanaka, Yuji Kumagai, Yoshiro Saito
Serum metabolites can reflect the diffusion/export of biochemicals from various organs. They can serve as biomarkers related to diseases and therapeutic efficacy/toxicity. While studies in Caucasians suggested that subject gender and age can affect circulating metabolite profiles, the Japanese population has not been surveyed. Our objective was to delineate gender- and age-associated differences in serum metabolite profiles among Japanese populations. Using a mass spectrometry-based global metabolomics approach, 516 endogenous metabolites were detected in sera from Japanese individuals...
2016: Biological & Pharmaceutical Bulletin
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