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steroid profiling mass spectrometry

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https://www.readbyqxmd.com/read/28105335/detection-of-stanozolol-in-the-urine-of-athletes-at-a-pg-level-the-possibility-of-passive-exposure
#1
Christina Tsitsimpikou, Konstantinos Tsarouhas, Demetrios A Spandidos, Aristides M Tsatsakis
Stanozolol is a synthetic heterocyclic steroid with anabolic and androgenic properties, which has been abused by several high-profile professional athletes. Stanozolol is also used in veterinary medicine to increase appetite, cause weight gain and treat certain types of anemia. The detection of stanozolol metabolites in human urine for doping control purposes depends on the analytical method applied. The most commonly applied methods in the World Anti-Doping Agency (WADA)-accredited doping control laboratories are gas chromatography/high-resolution mass spectrometry (GC/HRMS) or gas chromatography-mass spectrometry (GC/MS/MS)...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28076306/parallel-diurnal-fluctuation-of-testosterone-androstenedione-dehydroepiandrosterone-and-17ohprogesterone-as-assessed-in-serum-and-saliva-validation-of-a-novel-liquid-chromatography-tandem-mass-spectrometry-method-for-salivary-steroid-profiling
#2
Marco Mezzullo, Alessia Fazzini, Alessandra Gambineri, Guido Di Dalmazi, Roberta Mazza, Carla Pelusi, Valentina Vicennati, Renato Pasquali, Uberto Pagotto, Flaminia Fanelli
BACKGROUND: Salivary androgen testing represents a valuable source of biological information. However, the proper measurement of such low levels is challenging for direct immunoassays, lacking adequate accuracy. In the last few years, many conflicting findings reporting low correlation with the serum counterparts have hampered the clinical application of salivary androgen testing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) makes it possible to overcome previous analytical limits, providing new insights in endocrinology practice...
January 11, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28074002/metabolic-profiling-in-formalin-fixed-and-paraffin-embedded-prostate-cancer-tissues
#3
Stefano Cacciatore, Giorgia Zadra, Clyde Bango, Kathryn L Penney, Svitlana Tyekucheva, Oscar Yanes, Massimo Loda
: Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines...
January 10, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/27999117/performance-of-mass-spectrometry-steroid-profiling-for-diagnosis-of-polycystic-ovary-syndrome
#4
D J Handelsman, H J Teede, R Desai, R J Norman, L J Moran
STUDY QUESTION: How well does multi-analyte steroid mass spectrometry (MS) profiling classify women with and without polycystic ovary syndrome (PCOS)? SUMMARY ANSWER: Our liquid chromatography MS (LC-MS) steroid profiling only minimally improves discrimination of women with and without PCOS compared with a direct testosterone immunoassay (T_IA) and the free androgen index (FAI). WHAT IS KNOWN ALREADY: Blood testosterone measured by direct (non-extraction) immunoassay overlaps between women with and without PCOS...
December 19, 2016: Human Reproduction
https://www.readbyqxmd.com/read/27993082/stereoselective-degradation-of-estradiol-and-trenbolone-isomers-in-alluvial-sediment
#5
Yun Zhang, Daniel D Snow, Shannon L Bartelt-Hunt
Stereoisomers of estradiol (E2) or trenbolone (TB) can occur together in the environment receiving human or livestock wastes. However, the effect of their co-occurrence on persistence has not been well elucidated. A sandy and a silt loam sediment were used to establish microcosms with α- and β-isomers of E2 or TB spiked individually and together. Sediments were sampled periodically and analyzed for E2 and TB isomers and their transformation products using derivatization gas chromatography-mass spectrometry...
December 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27941603/analysis-of-metabolites-in-stem-parasitic-plant-interactions-interaction-of-cuscuta-momordica-versus-cassytha-ipomoea
#6
Takeshi Furuhashi, Takemichi Nakamura, Koji Iwase
Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha-Ipomoea and the Cuscuta-Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography), and conducted GC-MS (gas chromatography-mass spectrometry) analysis for polar metabolites (e...
December 7, 2016: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/27934105/simultaneous-analysis-of-free-and-sulfated-steroids-by-liquid-chromatography-mass-spectrometry-with-selective-mass-spectrometric-scan-modes-and-polarity-switching
#7
Su Hyeon Lee, Nanhee Lee, Youngmin Hong, Bong Chul Chung, Man Ho Choi
Sulfated steroids can act as a latent form of active free steroids, coexisting with them in biological specimens. To evaluate the metabolic significance of free and sulfated steroid species, a simultaneous analysis of eight free steroids [cholesterol, pregnenolone, 17α-hydroxypregnenolone, progesterone, 17α-hydroxyprogesterone, dehydroepiandrosterone (DHEA), testosterone, and 17β-estradiol] and four biologically relevant sulfated steroids was developed and validated, using selected-ion and multiple-reaction monitoring modes coupled to polarity-switching liquid chromatography/mass spectrometry (LC/MS)...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27881557/plasma-metabolomics-implicate-modified-transfer-rnas-and-altered-bioenergetics-in-the-outcome-of-pulmonary-arterial-hypertension
#8
Christopher J Rhodes, Pavandeep Ghataorhe, John Wharton, Kevin C Rue-Albrecht, Charaka Hadinnapola, Geoffrey Watson, Marta Bleda, Matthias Haimel, Gerry Coghlan, Paul A Corris, Luke S Howard, David G Kiely, Andrew J Peacock, Joanna Pepke-Zaba, Mark Toshner, Stephen J Wort, J Simon R Gibbs, Allan Lawrie, Stefan Gräf, Nicholas W Morrell, Martin R Wilkins
BACKGROUND: -Pulmonary arterial hypertension (PAH) is a heterogeneous disorder with high mortality. METHODS: -We conducted a comprehensive study of plasma metabolites using ultra-performance liquid chromatography mass-spectrometry to (1) identify patients at high risk of early death, (2) identify patients who respond well to treatment and (3) provide novel molecular insights into disease pathogenesis. RESULTS: -53 circulating metabolites distinguished well-phenotyped patients with idiopathic or heritable PAH (n=365) from healthy controls (n=121) following correction for multiple testing (p<7...
November 21, 2016: Circulation
https://www.readbyqxmd.com/read/27845856/modified-release-and-conventional-glucocorticoids-and-diurnal-androgen-excretion-in-congenital-adrenal-hyperplasia
#9
Christopher M Jones, Ashwini Mallappa, Nicole Reisch, Nikolaos Nikolaou, Nils Krone, Beverly A Hughes, Donna M O'Neil, Martin J Whitaker, Jeremy W Tomlinson, Karl-Heinz Storbeck, Deborah P Merke, Richard J Ross, Wiebke Arlt
CONTEXT: The classic androgen synthesis pathway proceeds via DHEA, androstenedione and testosterone to 5α-dihydrotestosterone (DHT). However, DHT synthesis can also be achieved by an alternative pathway originating from 17α-hydroxyprogesterone (17OHP), which accumulates in congenital adrenal hyperplasia (CAH). Similarly, recent work has highlighted androstenedione-derived 11-oxygenated 19-carbon steroids as active androgens and, in CAH, androstenedione is generated directly from 17OHP...
November 15, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27842983/a-novel-hplc-mrm-strategy-to-discover-unknown-and-long-term-metabolites-of-stanozolol-for-expanding-analytical-possibilities-in-doping-control
#10
Zhe Wang, Xinmiao Zhou, Xin Liu, Ying Dong, Jinlan Zhang
Stanozolol is one of the most commonly abused anabolic androgenic steroids (AAS) by athletes and usually detected by its parent drug and major metabolites. However, its metabolic pathway is complex, varied and individually different, it is important to characterize its overall metabolic profiles and discover new and long-term metabolites for the aims of expanding detection windows. High performance liquid chromatography coupled with triple quadrupole mass spectrometer (HPLC-MS/MS) was used to analyze the human urine after oral administration of stanozolol...
January 1, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/27827563/analytical-strategies-to-detect-enobosarm-administration-in-bovines
#11
Nora Cesbron, Alexandre Sydor, Mylène Penot, Stéphanie Prevost, Bruno Le Bizec, Gaud Dervilly-Pinel
Selective androgen receptor modulators (SARMs) are a novel class of androgen receptor ligands. They are intended to exhibit the same kind of effects as androgenic drugs, like anabolic steroids, but be much more selective in their action, targeting particular tissues without any undesirable effects on others. While the main applications of these synthetic substances are for therapeutic purposes, they also have a high potential for misuse in veterinary practice and the sporting world. In order to guarantee the consumers with food from animal origin free from any residues of such compounds, analytical strategies are required to ensure safe food and also enable fair trade between producers...
November 9, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27770750/oxidative-defense-metabolites-induced-by-salinity-stress-in-roots-of-salicornia-herbacea
#12
Seung Jae Lee, Eun-Mi Jeong, Ah Young Ki, Kyung-Seo Oh, Joseph Kwon, Jae-Hyuk Jeong, Nam-Jin Chung
High salinity is a major abiotic stress that affects the growth and development of plants. This type of stress can influence flowering, the production of crops, defense mechanisms and other physiological processes. Previous studies have attempted to elucidate salt-tolerance mechanisms to improve plant growth and productivity in the presence of sodium chloride. One such plant that has been studied in detail is Salicornia, a well-known halophyte, which has adapted to grow in the presence of high salt. To further the understanding of how Salicornia grows and develops under high saline conditions, Salicornia herbacea (S...
November 1, 2016: Journal of Plant Physiology
https://www.readbyqxmd.com/read/27750143/exploring-the-neural-mechanisms-of-finasteride-a-proteomic-analysis-in-the-nucleus-accumbens
#13
Alessio Soggiu, Cristian Piras, Viviana Greco, Paola Devoto, Andrea Urbani, Luigino Calzetta, Marco Bortolato, Paola Roncada
The enzyme 5α-reductase (5αR) catalyzes the conversion of progesterone and testosterone into neuroactive steroids implicated in a wide array of behavioral functions. The prototypical 5αR inhibitor, finasteride (FIN), is clinically approved for the treatment of androgenic alopecia and benign prostatic hyperplasia. Recent evidence has shown that FIN, albeit generally well tolerated, can induce untoward psychological effects in a subset of patients; furthermore, this drug may have therapeutic efficacy for a number of different neuropsychiatric conditions, ranging from Tourette syndrome to schizophrenia...
December 2016: Psychoneuroendocrinology
https://www.readbyqxmd.com/read/27717154/application-of-testosterone-to-epitestosterone-ratio-to-horse-urine-a-complimentary-approach-to-detect-the-administrations-of-testosterone-and-its-pro-drugs-in-thoroughbred-geldings
#14
Marjaana Viljanto, James Scarth, Pamela Hincks, Lynn Hillyer, Adam Cawley, Craig Suann, Glenys Noble, Christopher J Walker, Andrew T Kicman, Mark C Parkin
Detection of testosterone and/or its pro-drugs in the gelding is currently regulated by the application of an international threshold for urinary testosterone of 20 ng/mL. The use of steroid ratios may provide a useful supplementary approach to aid in differentiating between the administration of these steroids and unusual physiological conditions that may result in atypically high testosterone concentrations. In the current study, an ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method was developed to quantify testosterone (T) and epitestosterone (E)...
October 7, 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27714988/intranasal-delivery-of-natesto%C3%A2-testosterone-gel-and-its-effects-on-doping-markers
#15
Geoffrey D Miller, Vinod Nair, M Scott Morrison, Maggie Summers, Stuart E Willick, Daniel Eichner
The laboratory profile of intranasal testosterone gel has not been previously reported from an anti-doping perspective. Because intranasal testosterone gel is newly available as a commercial product, we sought to examine the laboratory parameters following administration of this formulation, with particular attention to anti-doping guidelines. Five healthy and active male subjects were administered testosterone intranasal gel three times daily for four weeks, using a pattern of five consecutive days on, two days off...
November 2016: Drug Testing and Analysis
https://www.readbyqxmd.com/read/27669235/new-potential-biomarker-for-methasterone-misuse-in-human-urine-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#16
Jianli Zhang, Jianghai Lu, Yun Wu, Xiaobing Wang, Youxuan Xu, Yinong Zhang, Yan Wang
In this study, methasterone urinary metabolic profiles were investigated by liquid chromatography quadrupole time of flight mass spectrometry (LC-QTOF-MS) in full scan and targeted MS/MS modes with accurate mass measurement. A healthy male volunteer was asked to take the drug and liquid-liquid extraction was employed to process urine samples. Chromatographic peaks for potential metabolites were hunted out with the theoretical [M - H](-) as a target ion in a full scan experiment and actual deprotonated ions were studied in targeted MS/MS experiment...
September 24, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27649607/chemical-composition-of-ethanolic-extracts-of-some-wild-mushrooms-from-tanzania-and-their-medicinal-potentials
#17
Baraka Luca Chelela, Musa Chacha, Athanasia Matemu
The ethanolic extracts of 5 edible and inedible wild mushrooms collected from the Southern Highlands of Tanzania were characterized by gas chromatography-mass spectrometry. A total of 75 chemical compounds were obtained, mainly fatty acids, carotenoids, alkaloids, phenols, terpernes, steroids, pyranoside, saccharides, and amino acids. Chemical compounds were identified from the ethanolic extract of Russula cellulata, R. kivuensis, Lactarius densifolius, L. gymnocarpoides, and Lactarius sp. In addition, mass spectra of 4 major groups of compounds were also determined...
2016: International Journal of Medicinal Mushrooms
https://www.readbyqxmd.com/read/27648966/body-fat-mass-is-associated-with-ratio-of-steroid-metabolites-reflecting-17-20-lyase-activity-in-prepubertal-girls
#18
Shin-Hye Kim, Ju-Yeon Moon, Hironobu Sasano, Man Ho Choi, Mi-Jung Park
CONTEXT: Pediatric obesity has been related to hyperandrogenism and premature adrenarche in previous studies. However, little is known regarding the association between body fat mass and steroidogenic enzyme activities in children. OBJECTIVE: To examine whether body fat mass is associated with serum steroid profiles in girls. DESIGN, PARTICIPANTS, AND SETTING: We enrolled 242 girls (125 prepubertal, 117 pubertal; age, 7-13 years). Early morning blood samples were drawn at a university hospital to measure serum steroid profiles using gas chromatography-mass spectrometry, and steroidogenic enzyme activities were assessed from the ratios of steroid metabolites...
December 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27582398/eicosanoid-mediators-in-the-airway-inflammation-of-asthmatic-patients-what-is-new
#19
REVIEW
Marek Sanak
Lipid mediators contribute to inflammation providing both pro-inflammatory signals and terminating the inflammatory process by activation of macrophages. Among the most significant biologically lipid mediators, these are produced by free-radical or enzymatic oxygenation of arachidonic acid named "eicosanoids". There were some novel eicosanoids identified within the last decade, and many of them are measurable in clinical samples by affordable chromatography-mass spectrometry equipment or sensitive immunoassays...
November 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27571716/effects-of-azocyclotin-on-gene-transcription-and-steroid-metabolome-of-hypothalamic-pituitary-gonad-axis-and-their-consequences-on-reproduction-in-zebrafish-danio-rerio
#20
You-Ning Ma, Chu-Yan Cao, Qiang-Wei Wang, Wen-Jun Gui, Guo-Nian Zhu
The widely used organotins have the potential to disrupt the endocrine system, but little is known of underlying mechanisms of azocyclotin toxicity in fish. The objective of the present study was to investigate the impact of azocyclotin on reproduction in zebrafish. Adult zebrafish were exposed to 0.09 and 0.45μg/L azocyclotin for 21days, and effects on steroid hormones and mRNA expression of the genes belonging to the hypothalamic-pituitary-gonad (HPG) axis were investigated. Mass spectrometry methodology was developed to profile steroids within the metabolome of the gonads...
October 2016: Aquatic Toxicology
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