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steroid profiling mass spectrometry

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https://www.readbyqxmd.com/read/29766657/drug-drug-interaction-and-doping-effect-of-non-prohibited-drugs-on-the-urinary-excretion-profile-of-methandienone
#1
Monica Mazzarino, Franziska L Khevenhüller-Metsch, Ilaria Fiacco, Maria Kristina Parr, Xavier de la Torre, Francesco Botrè
The potential consequences of drug-drug interactions on the excretion profile of the anabolic androgenic steroid methandienone (17β-hydroxy-17α-methylandrosta-1,4-dien-3-one) are discussed here. More specifically, we have evaluated by in vitro and in vivo experiments the effects of seven non-prohibited drugs (fluconazole, ketoconazole, itraconazole, miconazole, fluoxetine, paroxetine and nefazodone) on the main metabolic pathways of methandienone. These are selected among those most commonly used by the athletes...
May 15, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29755920/patterns-of-chemical-diversity-in-the-marine-ascidian-phallusia-spp-anti-tumor-activity-and-metabolic-pathway-inhibiting-steroid-biosynthesis
#2
Satheesh Kumar Palanisamy, Velusamy Arumugam, Magesh D Peter, Umamaheswari Sundaresan
The complex nature of marine biodiversity is partially responsible for the lack of studies in Indian ascidian species, which often target a small number of novel biomolecules. We performed untargeted metabolomics using gas chromatography-mass spectrometry (GC-MS) in two invasive ascidian species to investigate the inter-specific chemical diversity of Phallusia nigra and P. arabica in search of drug-like properties and metabolic pathways. The chemical profiling of individual ascidian species was obtained using GC-MS, and the metabolites were determined by searching in NIST library and literature data...
May 2018: 3 Biotech
https://www.readbyqxmd.com/read/29699872/methods-in-endogenous-steroid-profiling-a-comparison-of-gas-chromatography-mass-spectrometry-gc-ms-with-supercritical-fluid-chromatography-tandem-mass-spectrometry-sfc-ms-ms
#3
Juliane Teubel, Bernhard Wüst, Carola G Schipke, Oliver Peters, Maria Kristina Parr
In various fields of endocrinology, the determination of steroid hormones synthesised by the human body plays an important role. Research on central neurosteroids has been intensified within the last years, as they are discussed as biomarkers for various cognitive disorders. Their concentrations in cerebrospinal fluid (CSF) are considered to be regulated independently from peripheral fluids. For that reason, the challenging matrix CSF becomes a very interesting specimen for analysis. Concentrations are expected to be very low and available amount of CSF is limited...
April 17, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29694956/multiples-of-median-transformed-normalized-reference-ranges-of-steroid-profiling-data-independent-of-age-sex-and-units
#4
Dominika Zalas, Thomas Reinehr, Marek Niedziela, Christoph Borzikowsky, Maciej Flader, Gunter Simic-Schleicher, Halit Ilker Akkurt, Sabine Heger, Nadine Hornig, Paul-Martin Holterhus, Alexandra E Kulle
BACKGROUND/AIMS: The high complexity of pediatric reference ranges across age, sex, and units impairs clinical application and comparability of steroid hormone data, e.g., in congenital adrenal hyperplasia (CAH). We developed a multiples-of-median (MoM) normalization tool to overcome this major drawback in pediatric endocrinology. METHODS: Liquid chromatography tandem mass spectrometry data comprising 10 steroid hormones representing 905 controls (555 males, 350 females, 0 to > 16 years) from 2 previous datasets were MoM transformed across age and sex...
April 25, 2018: Hormone Research in Pædiatrics
https://www.readbyqxmd.com/read/29689248/urinary-steroid-profiling-by-gas-chromatography-mass-spectrometry-early-features-of-malignancy-in-patients-with-adrenal-incidentalomas
#5
Z R Shafigullina, L I Velikanova, N V Vorokhobina, S B Shustov, A A Lisitsin, E V Malevanaia, M O Buinova, E A Bessonova, D O Kirsanov
No abstract text is available yet for this article.
April 21, 2018: Steroids
https://www.readbyqxmd.com/read/29674995/serum-steroid-ratio-profiles-in-prostate-cancer-a-new-diagnostic-tool-toward-a-personalized-medicine-approach
#6
Adriana Albini, Antonino Bruno, Barbara Bassani, Gioacchino D'Ambrosio, Giuseppe Pelosi, Paolo Consonni, Laura Castellani, Matteo Conti, Simone Cristoni, Douglas M Noonan
Background: Serum steroids are crucial molecules altered in prostate cancer (PCa). Mass spectrometry (MS) is currently the elected technology for the analysis of steroids in diverse biological samples. Steroids have complex biological pathways and stoichiometry and it is important to evaluate their quantitative ratio. MS applications to patient hormone profiling could lead to a diagnostic approach. Methods: Here, we employed the Surface Activated Chemical Ionization-Electrospray-NIST (SANIST) developed in our laboratories, to obtain quantitative serum steroid ratio relationship profiles with a machine learning Bayesian model to discriminate patients with PCa...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/29668912/ovine-placental-steroid-synthesis-and-metabolism-in-late-gestation
#7
Lawrence P Reynolds, Erin L Legacki, C Jo Corbin, Joel S Caton, Kimberly A Vonnahme, Scott Stanley, Alan J Conley
Steroid synthesis is required for pregnancy maintenance and for parturition but comparatively little is known about the major metabolic routes that influence circulating concentrations. Dietary intake changes progesterone and estradiol concentrations in pregnant ewes but whether this reflects placental synthesis is unknown. Progesterone metabolism by 5alpha-reduction is a major metabolic route in other species and can influence the onset of parturition. Therefore, studies were conducted to 1) determine placental enzyme activity, progesterone and estradiol measured by immuno-assay in late gestation ewes on low, moderate and high nutritional planes, 2) to assess the significance of 5alpha-reduction of progesterone in determining progesterone concentrations in late gestation ewes (gestation day 145) given finasteride to inhibit 5alpha-reductase metabolism...
April 14, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29627638/the-utility-of-ultra-high-performance-supercritical-fluid-chromatography-tandem-mass-spectrometry-uhpsfc-ms-ms-for-clinically-relevant-steroid-analysis
#8
REVIEW
Karl-Heinz Storbeck, Lorna Gilligan, Carl Jenkinson, Elizabeth S Baranowski, Jonathan L Quanson, Wiebke Arlt, Angela E Taylor
Liquid chromatography tandem mass spectrometry (LC-MS/MS) assays are considered the reference standard for serum steroid hormone analyses, while full urinary steroid profiles are only achievable by gas chromatography (GC-MS). Both LC-MS/MS and GC-MS have well documented strengths and limitations. Recently, commercial ultra-high performance supercritical fluid chromatography-tandem mass spectrometry (UHPSFC-MS/MS) systems have been developed. These systems combine the resolution of GC with the high-throughput capabilities of UHPLC...
March 20, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29627396/characterization-of-circulating-steroid-hormone-profiles-in-the-bottlenose-dolphin-tursiops-truncatus-by-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms
#9
Thomas M Galligan, Lori H Schwacke, Dorian S Houser, Randall S Wells, Teri Rowles, Ashley S P Boggs
Systemic steroid hormone measurements are often used in the assessment of reproductive, developmental, and stress physiology in vertebrates. In protected wildlife, such as the common bottlenose dolphin (Tursiops truncatus), these measures can provide critical information about health and fitness to aid in effective conservation and management. Circulating steroid hormone concentrations are typically measured by immunoassays, which have imperfect specificity and are limited to the measurement of a single hormone per assay...
April 5, 2018: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/29608891/change-of-the-5%C3%AE-5%C3%AE-ratio-of-urinary-steroid-metabolites-in-benign-prostatic-hyperplasia-patients-treated-with-dutasteride
#10
Takahiro Maeda, Eiji Kikuchi, Masanori Hasegawa, Keiko Homma, Yota Yasumizu, Seiya Hattori, Takeo Kosaka, Kazunobu Shinoda, Akira Miyajima, Mototsugu Oya
BACKGROUND: The effects of the administration of dutasteride (DUT) on steroid metabolite pathways in BPH patients have not been examined. METHODS: Urine and blood samples as well as clinical parameters were prospectively collected after the administration of DUT to 60 BPH patients, and after its withdrawal in another set of 25 BPH patients. Urine samples were assessed using gas chromatography/mass spectrometry for the urinary steroid profile (USP), which simultaneously measures 63 steroid metabolites...
May 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29605636/rapid-effects-of-17%C3%AE-estradiol-on-aggressive-behavior-in-songbirds-environmental-and-genetic-influences
#11
REVIEW
Sarah A Heimovics, Jennifer R Merritt, Cecilia Jalabert, Chunqi Ma, Donna L Maney, Kiran K Soma
17β-estradiol (E2 ) has numerous rapid effects on the brain and behavior. This review focuses on the rapid effects of E2 on aggression, an important social behavior, in songbirds. First, we highlight the contributions of studies on song sparrows, which reveal that seasonal changes in the environment profoundly influence the capacity of E2 to rapidly alter aggressive behavior. E2 administration to male song sparrows increases aggression within 20 min in the non-breeding season, but not in the breeding season...
March 29, 2018: Hormones and Behavior
https://www.readbyqxmd.com/read/29599864/urinary-steroid-profile-in-ironman-triathletes
#12
Marta Marcos-Serrano, Guillermo Olcina, Carmen Crespo, Darrell Brooks, Rafael Timon
The aim of this study was to determine variations in the urinary steroid profile of triathletes following an Ironman event. A total of 10 male participants (age = 36.0 ± 1.27 years; body height = 179.29 ± 10.77 cm; body mass = 74.50 ± 1.04 kg) completed an Ironman Championship. Urine samples were collected before, immediately after, and 24 hours following the race. Gas chromatography-mass spectrometry (GC/MS) was used to detect and quantify catabolic and anabolic hormones: Androsterone, Dehydroepiandrosteone (DHEA), Androstenedione and Testosterone (T), Betaestradiol, Estrone, Progesterone, Cortisol (C), Cortisone, Tetrahydrocortisol (THE) and Tetrahydrocortisone (THF)...
March 2018: Journal of Human Kinetics
https://www.readbyqxmd.com/read/29575649/detection-and-pharmacokinetics-of-grapiprant-following-oral-administration-to-exercised-thoroughbred-horses
#13
Heather K Knych, Kelsey Seminoff, Dan S McKemie
Traditional therapeutic options for the treatment of lameness associated with inflammation in performance horses include administration of cyclooxygenase enzyme inhibiting non-steroidal anti-inflammatory drugs (NSAID). As long-term use of these drugs can adversely impact the health of the horse, anti-inflammatories with a more favorable safety profile are warranted. Grapiprant is a newly approved non-cyclooxygenase inhibiting NSAID that has demonstrated efficacy and safety in other species and which may be a valuable alternative to traditional NSAIDs used in the horse...
March 25, 2018: Drug Testing and Analysis
https://www.readbyqxmd.com/read/29573734/a-systematic-data-acquisition-and-mining-strategy-for-chemical-profiling-of-aster-tataricus-rhizoma-ziwan-by-uhplc-q-tof-ms-and-the-corresponding-anti-depressive-activity-screening
#14
Yupeng Sun, Li Li, Man Liao, Min Su, Changchen Wan, Lantong Zhang, Hailin Zhang
In this study, a systematic data acquisition and mining strategy aimed at the traditional Chinese medicine (TCM) complex system based on ultra high-performance liquid chromatography coupled with quadrupole time of flight mass spectrometry (UHPLC-Q-TOF-MS) was reported. The workflow of this strategy is as follows: First, the high resolution mass data are acquired by both data-dependent acquisition mode (DDA) and data-independent acquisition mode (DIA). Then a global data mining that combined targeted and non-targeted compound finding is applied to analyze mass spectral data...
March 12, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29568663/tissue-based-metabolite-profiling-and-qualitative-comparison-of-two-species-of-achyranthes-roots-by-use-of-uhplc-qtof-ms-and-laser-micro-dissection
#15
Yogini Jaiswal, Zhitao Liang, Alan Ho, Hubiao Chen, Leonard Williams, Zhongzhen Zhao
Achyranthes bidentata and Achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine (TCM) and Ayurveda. A. bidentata is reported to be one of the rare and extensively exploited medicinal plant species that face the issue of being endangered. Finding qualitative substitute with identical phyto-constituents contributing to similar composition and pharmacological benefits will help in reducing the burden of exploitation of the natural habitats of such plants...
February 2018: Journal of Pharmaceutical Analysis
https://www.readbyqxmd.com/read/29562689/gc-ms-based-endometabolome-analysis-differentiates-prostate-cancer-from-normal-prostate-cells
#16
Ana Rita Lima, Ana Margarida Araújo, Joana Pinto, Carmen Jerónimo, Rui Henrique, Maria de Lourdes Bastos, Márcia Carvalho, Paula Guedes de Pinho
Prostate cancer (PCa) is an important health problem worldwide. Diagnosis and management of PCa is very complex because the detection of serum prostate specific antigen (PSA) has several drawbacks. Metabolomics brings promise for cancer biomarker discovery and for better understanding PCa biochemistry. In this study, a gas chromatography-mass spectrometry (GC-MS) based metabolomic profiling of PCa cell lines was performed. The cell lines include 22RV1 and LNCaP from PCa with androgen receptor (AR) expression, DU145 and PC3 (which lack AR expression), and one normal prostate cell line (PNT2)...
March 19, 2018: Metabolites
https://www.readbyqxmd.com/read/29548683/metabolomic-profile-of-amniotic-fluid-and-wheezing-in-the-first-year-of-life-a-healthy-birth-cohort-study
#17
Silvia Carraro, Eugenio Baraldi, Giuseppe Giordano, Paola Pirillo, Matteo Stocchero, Michiel Houben, Louis Bont
OBJECTIVES: To apply metabolomic analysis of amniotic fluid in a discovery cohort to see whether a specific biochemical-metabolic profile at birth is associated with the subsequent onset of wheezing over the first year of life. STUDY DESIGN: This prospective exploratory study was conducted in a healthy term-born Dutch cohort recruited at 2 hospitals in Utrecht (UMCU, Utrecht, and Diakonessenhuis, Utrecht), The Netherlands. A metabolomic approach based on mass spectrometry was applied to analyze 142 amniotic fluid samples collected at birth...
May 2018: Journal of Pediatrics
https://www.readbyqxmd.com/read/29546058/uplc-qtof-ms-based-serum-metabolomic-profiling-analysis-reveals-the-molecular-perturbations-underlying-uremic-pruritus
#18
Qiong Wu, Huan Zhang, Jia-Rong Ding, Zhan-Ying Hong, Hao Wu, Zhen-Yu Zhu, Zhi-Yong Guo, Yi-Feng Chai
As one of the most troublesome complications in patients with chronic renal disease, the etiology of uremic pruritus remains unknown, and the current therapeutic approaches are limited and unsatisfactory. To identify potential biomarkers for improving diagnosis and treatment and obtain a better understanding of the pathogenesis of uremic pruritus, we compared serum metabolome profiles of severe uremic pruritus (HUP) patients with mild uremic pruritus (LUP) patients using ultraperformance liquid chromatography-quadruple time-of-flight mass spectrometry (UPLC-QTOF MS)...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29528626/collision-cross-section-ccs-database-an-additional-measure-to-characterize-steroids
#19
Maykel Hernández-Mesa, Bruno Le Bizec, Fabrice Monteau, Ana M García-Campaña, Gaud Dervilly-Pinel
Ion mobility spectrometry enhances the performance characteristics of liquid chromatography-mass spectrometry workflows intended to steroid profiling by providing a new separation dimension and a novel characterization parameter, the so-called collision cross section (CCS). This work proposes the first CCS database for 300 steroids (i.e., endogenous, including phase I and phase II metabolites, and exogenous synthetic compounds), which involves 1080 ions and covers the CCS of 127 androgens, 84 estrogens, 50 corticosteroids, and 39 progestagens...
April 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29522931/salivary-cortisol-and-cortisone-responses-to-short-term-psychological-stress-challenge-in-late-adolescent-and-young-women-with-different-hyperandrogenic-states
#20
Marco Mezzullo, Flaminia Fanelli, Guido Di Dalmazi, Alessia Fazzini, Daniela Ibarra-Gasparini, Marianna Mastroroberto, Jenny Guidi, Antonio Maria Morselli-Labate, Renato Pasquali, Uberto Pagotto, Alessandra Gambineri
Hyperandrogenic disorders have been associated with psychological distress, reduced quality of life, anxiety and depression. The hypothalamic-pituitary-adrenal (HPA) axis plays a pivotal role in the adaptive response to stressor events. Salivary cortisol (SalF) and cortisone (SalE) testing have been proven to be useful in the evaluation of HPA-axis activity. This study investigated whether SalF and SalE responses to two putative stressor levels differed between the hyperandrogenic states in late adolescent and young women, thus measuring the HPA-axis adaptive response to acute stress events...
May 2018: Psychoneuroendocrinology
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