keyword
MENU ▼
Read by QxMD icon Read
search

steroid profiling mass spectrometry

keyword
https://www.readbyqxmd.com/read/28396286/altered-mitochondrial-and-peroxisomal-integrity-in-lipocalin-2-deficient-mice-with-hepatic-steatosis
#1
Anastasia Asimakopoulou, Annabelle Fülöp, Erawan Borkham-Kamphorst, Eddy Van de Leur, Nikolaus Gassler, Thorsten Berger, Birte Beine, Helmut E Meyer, Tak W Mak, Carsten Hopf, Corinna Henkel, Ralf Weiskirchen
Lipocalin-2 (LCN2) is a secreted adipokine that transports small hydrophobic molecules such as fatty acids and steroids. LCN2 limits bacterial growth by sequestering iron-containing siderophores and in mammalian liver protects against inflammation, infection, injury and other stressors. Because LCN2 modulates hepatic fat metabolism and homeostasis, we performed a comparative profiling of proteins and lipids of wild type (WT) and Lcn2-deficient mice fed either standard chow or a methionine- and choline-deficient (MCD) diet...
April 7, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28393582/insights-into-the-human-brain-proteome-disclosing-the-biological-meaning-of-protein-networks-in-cerebrospinal-fluid
#2
Paulo Bastos, Rita Ferreira, Bruno Manadas, Paula I Moreira, Rui Vitorino
Cerebrospinal fluid (CSF) is an excellent source of biological information regarding the nervous system, once it is in close contact and accurately reflects alterations in this system. Several studies have analyzed differential protein profiles of CSF samples between healthy and diseased human subjects. However, the pathophysiological mechanisms and how CSF proteins relate to diseases are still poorly known. By applying bioinformatics tools, we attempted to provide new insights on the biological and functional meaning of proteomics data envisioning the identification of putative disease biomarkers...
April 10, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/28382793/rna-sample-preparation-applied-to-gene-expression-profiling-for-the-horse-biological-passport
#3
Ludovic Bailly-Chouriberry, Florent Baudoin, Florence Cormant, Yohan Glavieux, Benoit Loup, Patrice Garcia, Marie-Agnès Popot, Yves Bonnaire
The improvement of doping control is an on-going race. Techniques to fight against doping are usually based on the direct detection of drugs or their metabolites by analytical methods such as chromatography hyphenated to mass spectrometry after ad hoc sample preparation. Nowadays, omic methods constitute an attractive development and advances have been achieved particularly by application of molecular biology tools for detection of anabolic androgenic steroids (AAS), erythropoiesis-stimulating agent (ESA) or to control human growth hormone misuses...
April 5, 2017: Drug Testing and Analysis
https://www.readbyqxmd.com/read/28356371/metabolomics-analysis-identifies-different-metabotypes-of-asthma-severity
#4
Stacey N Reinke, Héctor Gallart-Ayala, Cristina Gómez, Antonio Checa, Alexander Fauland, Shama Naz, Muhammad Anas Kamleh, Ratko Djukanović, Timothy S C Hinks, Craig E Wheelock
In this study, we sought to determine whether asthma has a metabolic profile and whether this profile is related to disease severity.We characterised the serum from 22 healthy individuals and 54 asthmatics (12 mild, 20 moderate, 22 severe) using liquid chromatography-high-resolution mass spectrometry-based metabolomics. Selected metabolites were confirmed by targeted mass spectrometry assays of eicosanoids, sphingolipids and free fatty acids.We conclusively identified 66 metabolites; 15 were significantly altered with asthma (p≤0...
March 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28351628/coupling-of-gas-chromatography-and-electrospray-ionization-high-resolution-mass-spectrometry-for-the-analysis-of-anabolic-steroids-as-trimethylsilyl-derivatives-in-human-urine
#5
Eunju Cha, Eun Sook Jeong, Sangwon Cha, Jaeick Lee
In this study, gas chromatography (GC) was interfaced with high resolution mass spectrometry (HRMS) with electrospray ionization source (ESI) and the relevant parameters were investigated to enhance the ionization efficiency. In GC-ESI, the distances (x-, y- and z) and angle between the ESI needle, GC capillary column and MS orifice were set to 7 (x-distance), 4 (y-distance), and 1 mm (z-distance). The ESI spray solvent, acid modifier and nebulizer gas flow were methanol, 0.1% formic acid and 5 arbitrary units, respectively...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28351627/non-targeted-lc-ms-based-metabolomics-analysis-of-the-urinary-steroidal-profile
#6
Amelia Palermo, Francesco Botrè, Xavier de la Torre, Nicola Zamboni
The urinary steroidal fraction has been extensively explored as non-invasive alternative to monitor pathological conditions as well as to unveil the illicit intake of pseudo-endogenous anabolic steroids in sport. However, the majority of previous approaches involved the a priori selection of potentially relevant target analytes. Here we describe the non-targeted analysis of the urinary steroidal profiles. The workflow includes minimal sample pretreatment and normalization according to the specific gravity of urine, a 20 min reverse phase ultra-performance liquid chromatographic separation hyphenated to electrospray time-of-flight mass spectrometry...
April 29, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28326509/the-steroid-response-to-human-chorionic-gonadotropin-hcg-stimulation-in-men-with-klinefelter-syndrome-does-not-change-using-immunoassay-or-mass-spectrometry
#7
L Roli, D Santi, S Belli, S Tagliavini, S Cavalieri, M C De Santis, E Baraldi, F Fanelli, M Mezzullo, A R Granata, U Pagotto, R Pasquali, V Rochira, C Carani, M Simoni, T Trenti
PURPOSE: Liquid-chromatography tandem mass-spectrometry (LC-MS/MS) was developed in parallel to Immunoassays (IAs) and today is proposed as the "gold standard" for steroid assays. Leydig cells of men with Klinefelter syndrome (KS) are able to respond to human chorionic gonadotropin (hCG) stimulation, even if testosterone (T) production was impaired. The aim was to evaluate how results obtained by IAs and LC-MS/MS can differently impact on the outcome of a clinical research on gonadal steroidogenesis after hCG stimulation...
March 21, 2017: Journal of Endocrinological Investigation
https://www.readbyqxmd.com/read/28259259/endometriosis-is-associated-with-aberrant-metabolite-profiles-in-plasma
#8
Sophia Letsiou, Dirkje P Peterse, Amelie Fassbender, Margriet M Hendriks, Niels J van den Broek, Rudolf Berger, Dorien F O, Arne Vanhie, Alexandra Vodolazkaia, Anne Van Langendonckt, Jacques Donnez, Amy C Harms, Rob J Vreeken, Patrick G Groothuis, Marie-Madeleine Dolmans, Arjan B Brenkman, Thomas M D'Hooghe
OBJECTIVE: To identify metabolites that are associated with and predict the presence of endometriosis. DESIGN: Metabolomics study using state-of-the-art mass spectrometry approaches. SETTING: University hospital and universities. PATIENT(S): Twenty-five women with laparoscopically confirmed endometriosis (cases) and 19 women with laparoscopically documented absence of endometriosis (controls). None of the women included in this study had received oral contraception or GnRH agonists for a minimum of 1 month before blood collection...
March 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28249803/venomics-analyses-of-the-skin-secretion-of-dermatonotus-muelleri-preliminary-proteomic-and-metabolomic-profiling
#9
Ingrid Duarte Cavalcante, Marta Maria Antoniazzi, Carlos Jared, Osmindo R Pires, Juliana Mozer Sciani, Daniel Carvalho Pimenta
Dermatonotus muelleri is the sole species of the Dermatonotus genus and inhabits Argentina, Bolivia, Brazil and Paraguay. This animal exhibits an explosive reproductive behavior during the Southern spring months, which lasts only for five days. Moreover, this animal displays specific adaptations to the habitat resulting in the energy conservation needed during either the intense reproduction period or times of estivation. During dry seasons and/or food shortages D. muelleri can survive because its food specialization and ability to dig an underground chamber for protection...
February 27, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28235592/steroid-profiling-in-h295r-cells-to-identify-chemicals-potentially-disrupting-the-production-of-adrenal-steroids
#10
Petra Strajhar, David Tonoli, Fabienne Jeanneret, Raphaella M Imhof, Vanessa Malagnino, Melanie Patt, Denise V Kratschmar, Julien Boccard, Serge Rudaz, Alex Odermatt
The validated OECD test guideline 456 based on human adrenal H295R cells promotes measurement of testosterone and estradiol production as read-out to identify potential endocrine disrupting chemicals. This study aimed to establish optimal conditions for using H295R cells to detect chemicals interfering with the production of key adrenal steroids. H295R cells' supernatants were characterized by liquid chromatography-mass spectrometry (LC-MS)-based steroid profiling, and the influence of experimental conditions including time and serum content was assessed...
April 15, 2017: Toxicology
https://www.readbyqxmd.com/read/28222414/urinary-steroid-metabolites-in-a-case-of-florid-ectopic-cushing-s-syndrome-and-clinical-correlations
#11
Angelos Kyriacou, Karolina M Stepien, Basil Issa
A 51-year old woman was admitted with multiple cerebral, pulmonary and intra-abdominal abscesses. The combination of apparent immunosuppression, obesity, diabetes mellitus, hypertension, hypokalaemia, osteoporotic fractures and bilateral shoulder avascular necrosis led to a clinical diagnosis of Cushing's syndrome (CS). This was biochemically confirmed as follows: midnight serum cortisol 4275 nmol/L (60-250), non-suppressed overnight dexamethasone suppression test, raised salivary cortisol 716 nmol/L (5-46) and ACTH 639 ng/L (0-46)...
October 2016: Hormones: International Journal of Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28146078/metabolomic-biomarkers-in-urine-of-cushing-s-syndrome-patients
#12
Alicja Kotłowska, Tomasz Puzyn, Krzysztof Sworczak, Piotr Stepnowski, Piotr Szefer
Cushing's syndrome (CS) is a disease which results from excessive levels of cortisol in the human body. The disorder is associated with various signs and symptoms which are also common for the general population not suffering from compound hypersecretion. Thus, more sensitive and selective methods are required for the diagnosis of CS. This follow-up study was conducted to determine which steroid metabolites could serve as potential indicators of CS and possible subclinical hypercortisolism in patients diagnosed with so called non-functioning adrenal incidentalomas (AIs)...
January 29, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28105335/detection-of-stanozolol-in-the-urine-of-athletes-at-a-pg-level-the-possibility-of-passive-exposure
#13
Christina Tsitsimpikou, Konstantinos Tsarouhas, Demetrios A Spandidos, Aristides M Tsatsakis
Stanozolol is a synthetic heterocyclic steroid with anabolic and androgenic properties, which has been abused by several high-profile professional athletes. Stanozolol is also used in veterinary medicine to increase appetite, cause weight gain and treat certain types of anemia. The detection of stanozolol metabolites in human urine for doping control purposes depends on the analytical method applied. The most commonly applied methods in the World Anti-Doping Agency (WADA)-accredited doping control laboratories are gas chromatography/high-resolution mass spectrometry (GC/HRMS) or gas chromatography-mass spectrometry (GC/MS/MS)...
December 2016: Biomedical Reports
https://www.readbyqxmd.com/read/28076306/parallel-diurnal-fluctuation-of-testosterone-androstenedione-dehydroepiandrosterone-and-17ohprogesterone-as-assessed-in-serum-and-saliva-validation-of-a-novel-liquid-chromatography-tandem-mass-spectrometry-method-for-salivary-steroid-profiling
#14
Marco Mezzullo, Alessia Fazzini, Alessandra Gambineri, Guido Di Dalmazi, Roberta Mazza, Carla Pelusi, Valentina Vicennati, Renato Pasquali, Uberto Pagotto, Flaminia Fanelli
BACKGROUND: Salivary androgen testing represents a valuable source of biological information. However, the proper measurement of such low levels is challenging for direct immunoassays, lacking adequate accuracy. In the last few years, many conflicting findings reporting low correlation with the serum counterparts have hampered the clinical application of salivary androgen testing. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) makes it possible to overcome previous analytical limits, providing new insights in endocrinology practice...
January 11, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28074002/metabolic-profiling-in-formalin-fixed-and-paraffin-embedded-prostate-cancer-tissues
#15
Stefano Cacciatore, Giorgia Zadra, Clyde Bango, Kathryn L Penney, Svitlana Tyekucheva, Oscar Yanes, Massimo Loda
Metabolite profiling has significantly contributed to a deeper understanding of the biochemical metabolic networks and pathways in cancer cells. Metabolomics-based biomarker discovery would greatly benefit from the ability to interrogate retrospective annotated clinical specimens archived as formalin-fixed, paraffin-embedded (FFPE) material. Mass spectrometry-based metabolomic analysis was performed in matched frozen and FFPE human prostate cancers as well as isogenic prostate cancer cell lines. A total of 352 and 460 metabolites were profiled in human tissues and cell lines, respectively...
April 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/27999117/performance-of-mass-spectrometry-steroid-profiling-for-diagnosis-of-polycystic-ovary-syndrome
#16
D J Handelsman, H J Teede, R Desai, R J Norman, L J Moran
STUDY QUESTION: How well does multi-analyte steroid mass spectrometry (MS) profiling classify women with and without polycystic ovary syndrome (PCOS)? SUMMARY ANSWER: Our liquid chromatography MS (LC-MS) steroid profiling only minimally improves discrimination of women with and without PCOS compared with a direct testosterone immunoassay (T_IA) and the free androgen index (FAI). WHAT IS KNOWN ALREADY: Blood testosterone measured by direct (non-extraction) immunoassay overlaps between women with and without PCOS...
February 2017: Human Reproduction
https://www.readbyqxmd.com/read/27993082/stereoselective-degradation-of-estradiol-and-trenbolone-isomers-in-alluvial-sediment
#17
Yun Zhang, Daniel D Snow, Shannon L Bartelt-Hunt
Stereoisomers of estradiol (E2) or trenbolone (TB) can occur together in the environment receiving human or livestock wastes. However, the effect of their co-occurrence on persistence has not been well elucidated. A sandy and a silt loam sediment were used to establish microcosms with α- and β-isomers of E2 or TB spiked individually and together. Sediments were sampled periodically and analyzed for E2 and TB isomers and their transformation products using derivatization gas chromatography-mass spectrometry...
December 20, 2016: Environmental Science & Technology
https://www.readbyqxmd.com/read/27941603/analysis-of-metabolites-in-stem-parasitic-plant-interactions-interaction-of-cuscuta-momordica-versus-cassytha-ipomoea
#18
Takeshi Furuhashi, Takemichi Nakamura, Koji Iwase
Cuscuta and Cassytha are two well-known stem parasitic plant genera with reduced leaves and roots, inducing haustoria in their stems. Their similar appearance in the field has been recognized, but few comparative studies on their respective plant interactions are available. To compare their interactions, we conducted a metabolite analysis of both the Cassytha-Ipomoea and the Cuscuta-Momordica interaction. We investigated the energy charge of the metabolites by UFLC (ultra-high performance liquid chromatography), and conducted GC-MS (gas chromatography-mass spectrometry) analysis for polar metabolites (e...
December 7, 2016: Plants (Basel, Switzerland)
https://www.readbyqxmd.com/read/27934105/simultaneous-analysis-of-free-and-sulfated-steroids-by-liquid-chromatography-mass-spectrometry-with-selective-mass-spectrometric-scan-modes-and-polarity-switching
#19
Su Hyeon Lee, Nanhee Lee, Youngmin Hong, Bong Chul Chung, Man Ho Choi
Sulfated steroids can act as a latent form of active free steroids, coexisting with them in biological specimens. To evaluate the metabolic significance of free and sulfated steroid species, a simultaneous analysis of eight free steroids [cholesterol, pregnenolone, 17α-hydroxypregnenolone, progesterone, 17α-hydroxyprogesterone, dehydroepiandrosterone (DHEA), testosterone, and 17β-estradiol] and four biologically relevant sulfated steroids was developed and validated, using selected-ion and multiple-reaction monitoring modes coupled to polarity-switching liquid chromatography/mass spectrometry (LC/MS)...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27881557/plasma-metabolomics-implicates-modified-transfer-rnas-and-altered-bioenergetics-in-the-outcomes-of-pulmonary-arterial-hypertension
#20
Christopher J Rhodes, Pavandeep Ghataorhe, John Wharton, Kevin C Rue-Albrecht, Charaka Hadinnapola, Geoffrey Watson, Marta Bleda, Matthias Haimel, Gerry Coghlan, Paul A Corris, Luke S Howard, David G Kiely, Andrew J Peacock, Joanna Pepke-Zaba, Mark R Toshner, S John Wort, J Simon R Gibbs, Allan Lawrie, Stefan Gräf, Nicholas W Morrell, Martin R Wilkins
BACKGROUND: Pulmonary arterial hypertension (PAH) is a heterogeneous disorder with high mortality. METHODS: We conducted a comprehensive study of plasma metabolites using ultraperformance liquid chromatography mass spectrometry to identify patients at high risk of early death, to identify patients who respond well to treatment, and to provide novel molecular insights into disease pathogenesis. RESULTS: Fifty-three circulating metabolites distinguished well-phenotyped patients with idiopathic or heritable PAH (n=365) from healthy control subjects (n=121) after correction for multiple testing (P<7...
January 31, 2017: Circulation
keyword
keyword
73460
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"