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syphilis pcr

Michelle Shuel, Kristy Hayden, Kamran Kadkhoda, Raymond Sw Tsang
BACKGROUND: The province of Manitoba, Canada, population of about 1.3 million, has been experiencing increased incidence of syphilis cases since 2015. In this study we examined the detection of T. pallidum DNA in 354 clinical samples from 2012 to 2016; and determined molecular types and mutations conferring resistance to azithromycin in the PCR-positive samples. METHODS: T. pallidum DNA detection was done by PCR amplification of tpp47, bmp, and polA genes. Syphilis serology results were reviewed for the PCR-positive cases...
October 5, 2017: Sexually Transmitted Diseases
H Kassem Youssef, A Blind, F Chouta Ngaha, B Drenou, H Nojavan, C Michel
INTRODUCTION: Syphilis is a sexually transmitted disease that can affect numerous organs in its secondary or tertiary stages. We describe a case of secondary syphilis with pulmonary involvement and we present a literature review. PATIENTS AND METHODS: A 69-year-old male patient was admitted to hospital for dyspnoea and extended papular exanthema with palmoplantar involvement. The serological test for syphilis was positive. Ocular examination showed bilateral papillitis and retinal haemorrhage...
February 17, 2018: Annales de Dermatologie et de Vénéréologie
Anna Brischetto, Ian Gassiep, David Whiley, Robert Norton
There has been a resurgence of syphilis diagnoses in Australia. We investigated whether our Treponema pallidum polymerase chain reaction (PCR) test provides any additional diagnostic information over syphilis serology (chemi-luminescence immunoassay (CMIA), Treponema pallidum particle agglutination (TPPA) and rapid reagin (RPR) flocculation test). A retrospective audit was conducted of all T. pallidum PCR requests that came through our laboratory from January 2010 to June 2017; data collected included age, gender, site of swab, T...
February 14, 2018: Journal of Clinical Microbiology
Ju Hwan Seo, Joon Woo Lee, Daemyeong Cho
Background: The molecular diagnostics market can be broadly divided into PCR (rt-PCR, d-PCR), NGS(Next Generation Sequencing), Microarray, FISH(Fluorescent in situ-hybridization) and other categories, based on the diagnostic technique. Also, depending on the disease being diagnosed, the market can also be divided into cancer, infectious diseases, HIV/STDs (herpes, syphilis), and women's health issues such as breast cancer, cervical cancer, ovarian cancer, HPV(human papillomavirus), and vaginitis...
2018: Biomaterials Research
Guilherme Amaral Calvet, Edna Oliveira Kara, Silvana Pereira Giozza, Camila Helena Aguiar Bôtto-Menezes, Philippe Gaillard, Rafael Freitas de Oliveira Franca, Marcus Vinicius Guimarães de Lacerda, Marcia da Costa Castilho, Patrícia Brasil, Patrícia Carvalho de Sequeira, Maeve Brito de Mello, Ximena Pamela Diaz Bermudez, Kayvon Modjarrad, Robyn Meurant, Sihem Landoulsi, Adele Schwartz Benzaken, Ana Maria Bispo de Filippis, Nathalie Jeanne Nicole Broutet
BACKGROUND: Zika virus (ZIKV) has been identified in several body fluids of infected individuals. In most cases, it remained detected in blood from few days to 1 week after the onset of symptoms, and can persist longer in urine and in semen. ZIKV infection can have dramatic consequences such as microcephaly and Guillain-Barré syndrome. ZIKV sexual transmission has been documented. A better understanding of ZIKV presence and persistence across biologic compartments is needed to devise rational measures to prevent its transmission...
January 22, 2018: BMC Infectious Diseases
Kristina Adachi, Jiahong Xu, Nava Yeganeh, Margaret Camarca, Mariza G Morgado, D Heather Watts, Lynne M Mofenson, Valdilea G Veloso, Jose Henrique Pilotto, Esau Joao, Glenda Gray, Gerhard Theron, Breno Santos, Rosana Fonseca, Regis Kreitchmann, Jorge Pinto, Marisa M Mussi-Pinhata, Mariana Ceriotto, Daisy Maria Machado, Yvonne J Bryson, Beatriz Grinsztejn, Jack Moye, Jeffrey D Klausner, Claire C Bristow, Ruth Dickover, Mark Mirochnick, Karin Nielsen-Saines
BACKGROUND: Sexually transmitted infections (STIs) including Chlamydia trachomatis (CT), Neisseria gonorrhoeae (NG), Treponema pallidum (TP), and cytomegalovirus (CMV) may lead to adverse pregnancy and infant outcomes. The role of combined maternal STIs in HIV mother-to-child transmission (MTCT) was evaluated in mother-infant pairs from NICHD HPTN 040. METHODOLOGY: Urine samples from HIV-infected pregnant women during labor were tested by polymerase chain reaction (PCR) for CT, NG, and CMV...
2018: PloS One
More Mungati, Anna Machiha, Owen Mugurungi, Mufuta Tshimanga, Peter H Kilmarx, Justice Nyakura, Gerald Shambira, Vitalis Kupara, David A Lewis, Elizabeth Gonese, Beth A Tippett Barr, H Hunter Handsfield, Cornelis A Rietmeijer
BACKGROUND: In many countries, sexually transmitted infections (STIs) are treated syndromically. Thus, patients diagnosed as having genital ulcer disease (GUD) in Zimbabwe receive a combination of antimicrobials to treat syphilis, chancroid, lymphogranuloma venereum (LGV), and genital herpes. Periodic studies are necessary to assess the current etiology of GUD and assure the appropriateness of current treatment guidelines. MATERIALS AND METHODS: We selected 6 geographically diverse clinics in Zimbabwe serving high numbers of STI cases to enroll men and women with STI syndromes, including GUD...
January 2018: Sexually Transmitted Diseases
Cornelis A Rietmeijer, More Mungati, Anna Machiha, Owen Mugurungi, Vitalis Kupara, Luanne Rodgers, Peter H Kilmarx, Amy Herman Roloff, Elizabeth Gonese, Beth A Tippett-Barr, Gerald Shambira, David A Lewis, H Hunter Handsfield, Mufuta Tshimanga
INTRODUCTION: Sexually transmitted infections (STIs) are managed syndromically in most developing countries. In Zimbabwe, men presenting with urethral discharge are treated with a single intramuscular dose of kanamycin or ceftriaxone in combination with a week's course of oral doxycycline. This study was designed to assess the current etiology of urethral discharge and other STIs to inform current syndromic management regimens. METHODS: We conducted a study among 200 men with urethral discharge presenting at 6 regionally diverse STI clinics in Zimbabwe...
January 2018: Sexually Transmitted Diseases
Mao Hagihara, Yuka Yamagishi, Hideo Kato, Yuichi Shibata, Arufumi Shiota, Daisuke Sakanashi, Hiroyuki Suematsu, Hiroki Watanabe, Nobuhiro Asai, Yusuke Koizumi, Tatsuro Furui, Seiichiro Takahashi, Koji Izumi, Hiroshige Mikamo
Frequency of Treponema pallidum invasion into cerebrospinal fluid (CSF) has not been clear at this present. Since it is impossible to culture T. pallidum in vitro at this present, we need molecular based-approach to detect it in CSF. Additionally, neurosyphilis is usually a late sequela, however it might result in asymptomatic neurosyphilis even at primary or secondary syphilis. This study was to reveal the frequency of T. pallidum invasion into CSF especially at primary or secondary syphilis with polymerase chain reaction (PCR) test...
December 8, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Sukhum Silpa-Archa, Janine M Preble, C Stephen Foster
PURPOSE: To report the novel application of nontreponemal and treponemal antibody to confirm diagnosis of ocular syphilis from vitreous samples. METHODS: Two distinct case reports emphasizing the importance of confirmatory vitreous treponemal antibody. Multimodal imaging of patients was also applied. RESULTS: We report two distinct cases with positive serum treponemal antibody but opposing vitreous treponemal antibody results. One case with a positive vitreous test responded well to antisyphilitic treatment...
November 22, 2017: Retinal Cases & Brief Reports
S De Carolis, S Tabacco, F Rizzo, G Perrone, C Garufi, A Botta, S Salvi, P Benedetti Panici, A Lanzone
The present study investigated: (a) the presence of antiphospholipid antibodies and (b) the obstetric outcome in healthy pregnant women showing false-positive TORCH-Toxoplasmosis, Other: syphilis, varicella-zoster, Rubella, Cytomegalovirus (CMV), and Herpes infections-results. Data from 23 singleton healthy pregnancies with false-positive TORCH results were collected. Each woman was systematically screened for TORCH IgG and IgM during the pre-conception assessment and/or at the beginning of pregnancy. In the presence of IgM positivity, when indicated (CMV, toxoplasmosis, rubella, herpes simplex virus), IgG avidity was evaluated and, if possible, polymerase chain reaction was performed on an amniotic fluid sample in order to distinguish between primary infection or false positivity...
January 1, 2017: Lupus
Supriya D Mehta, Ashish K Pradhan, Stefan J Green, Ankur Naqib, Elijah Odoyo-June, Charlotte A Gaydos, Sheila Barry, Alan Landay, Robert C Bailey
We measured the microbial community structure of genital ulcers in women. Swabs from clinically detected ulcers were tested for HSV-2 and Treponema pallidum by polymerase chain reaction (PCR). HSV-2 and T. pallidum were detected by serum antibody testing. Microbial community structure was characterized by high-throughput 16 s rRNA gene amplicon sequencing. Multiple group testing and Elastic net and Lasso regressions identified taxa associated with differences in factors of interest. Among 49 ulcer specimens from 49 HSV-2 seropositive women, by PCR HSV-2 was recovered from 28 (57%) specimens and T...
November 13, 2017: Scientific Reports
Miguel Costa-Silva, Daniel Coutinho, Joana Sobrinho-Simões, Filomena Azevedo, Carmen Lisboa
BACKGROUND: Syphilis remains a major challenge and a complex diagnosis. We aim to evaluate the role of polymerase chain reaction (PCR) in Treponema pallidum (Tp) detection in various types of biological samples in the diagnosis of early syphilis. METHODS: We conducted a cross-sectional study including all attendees of the STI clinic with clinical suspicion of early syphilis. One or more specimens for the detection of Tp by PCR testing were collected. RESULTS: The overall sensitivity of Tp PCR test was 82...
October 31, 2017: International Journal of Dermatology
Kareem Moussa, Thuy Doan, Jay M Stewart, Jessica Shantha, John Gonzales, Nisha Acharya, Emmett T Cunningham
PURPOSE: To present a case of cytomegalovirus (CMV) retinitis associated with occlusive vasculopathy presenting as sudden unilateral loss of vision in a human immunodeficiency virus-negative elderly man. METHODS: Clinical case report and literature review. RESULTS: An 84-year-old Chinese man with diabetes mellitus and primary open-angle glaucoma was seen in consultation by our uveitis service for evaluation of sudden vision loss in the right eye...
September 20, 2017: Retinal Cases & Brief Reports
Afona Chernet, Andreas Neumayr, Christoph Hatz, Kerstin Kling, Véronique Sydow, Katharina Rentsch, Jürg Utzinger, Nicole Probst-Hensch, Hanspeter Marti, Beatrice Nickel, Niklaus D Labhardt
OBJECTIVES: Our study aimed at determining the prevalence of selected infectious diseases among recently arrived Eritrean refugees in Switzerland. METHODS: In this cross-sectional study, asymptomatic Eritrean migrants aged ≥16 years who arrived <24 months ago were recruited at refugee centres in Switzerland. Infectious disease screening included serology for HIV, hepatitis B and C, syphilis and schistosomiasis, polymerase chain reaction (PCR) for malaria, stool microscopy for helminths and intestinal protozoa and circulating cathodic antigen (CCA) testing in urine for schistosomiasis...
September 19, 2017: International Journal of Public Health
S Sultan, M I Nasir, S Rafiq, M A Baig, S Akbani, S M Irfan
BACKGROUND: Blood transfusion safety commences with healthy donor recruitment. The threat of transfusion transmitted infections is greatly minimized by serological tools but not entirely eliminated. Recently, nucleic-acid testing for blood donor screening has virtually eliminated this jeopardy. METHODS: This prospective study was conducted from February 2015 to February 2016. Samples from seronegative donors were run on multiplex assay (Cobas, S-201 system platform, Roche) in a batch of six [MP-NAT]...
August 2017: Malaysian Journal of Pathology
Josep Coll, Sebastián Videla, Agathe Leon, Arelly Ornelas, Felipe García, Emma Fernández, José Luis Blanco, Antonio Carrillo, Isabel Bravo, Michael Meulbroek, Francesc García-Cuyas, Victoria González, Jordi Casabona, Lorna Leal, Bonaventura Clotet, Christian Brander
OBJECTIVE: To provide data on incidence of early diagnosis of HIV infections and define prevalence and incidence of asymptomatic STI in MSM. METHODS: Prospective cohort study of HIV-uninfected MSM at high-risk for HIV-infection. Participants were selected through a risk-assessment questionnaire, and screened for HIV-infection (quarterly) and for other STIs (yearly): syphilis, hepatitis A, B and C (serology); C. trachomatis and N. gonorrhoeae in penis and rectum and Human Papillomavirus in anus and mouth (PCR)...
August 23, 2017: Clinical Microbiology and Infection
Kara Osbak, Said Abdellati, Achilleas Tsoumanis, Marjan Van Esbroeck, Luc Kestens, Tania Crucitti, Chris Kenyon
PURPOSE: We evaluated the Sekure rapid plasma reagin (RPR-S) (Sekisui Diagnostics) automated quantitative latex immunoturbidimetric assay performed on the SK500 clinical chemistry system for clinical appropriateness. METHODOLOGY: Syphilis-infected individuals and controls were recruited into a prospective cohort study conducted at a sexually transmitted infection clinic in Antwerp, Belgium. Sera collected at diagnosis (baseline) and at 3, 6, 9 and 12 months post-treatment were tested with RPR-S and Macro-Vue RPR card (RPR-C) (Becton Dickinson) assays; RPR-C was considered the reference test...
August 10, 2017: Journal of Medical Microbiology
P S Wozniak, J B Cantey, F Zeray, N K Leos, J S Sheffield, G D Wendel, P J Sánchez
OBJECTIVE: Infants whose mothers had syphilis during pregnancy were studied to determine how often exposed newborns with normal physical examinations and nonreactive nontreponemal serologic tests had abnormal laboratory or radiographic studies. STUDY DESIGN: Retrospective analysis of prospectively collected data from infants born to mothers with syphilis and had a normal examination and a nonreactive nontreponemal test. Some infants had IgM immunoblotting, PCR testing or rabbit infectivity testing (RIT) performed...
October 2017: Journal of Perinatology: Official Journal of the California Perinatal Association
Kuan Lai, Nicolas Pinto-Sander, Daniel Richardson, Shanshan Wei, Kang Zeng
Awareness of the spectrum of clinical manifestations of syphilis, especially uncommon changes, is essential for diagnosis and effective management of patients. A 48-year-old Han businessman presented to the ear, nose and throat surgeons with an eight-week history of epigastric pain, a four-week history of a widespread non-itchy rash including the scrotal skin and a one-week history of tinnitus and dizziness. On examination, he was afebrile with widespread lymphadenopathy and a maculopapular rash affecting his trunk and scrotum...
January 1, 2017: International Journal of STD & AIDS
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