keyword
MENU ▼
Read by QxMD icon Read
search

Dmc1

keyword
https://www.readbyqxmd.com/read/27883312/time-course-analysis-of-gene-expression-during-the-saccharomyces-cerevisiae-hypoxic-response
#1
Nasrine Bendjilali, Samuel MacLeon, Gurmannat Kalra, Stephen D Willis, A K M Nawshad Hossian, Erica Avery, Olivia Wojtowicz, Mark J Hickman
Many cells experience hypoxia, or low oxygen, and respond by dramatically altering gene expression. In the yeast S. cerevisiae, genes that respond are required for many oxygen-dependent cellular processes, such as respiration, biosynthesis and redox regulation. To more fully characterize the global response to hypoxia, we exposed yeast to hypoxic conditions, extracted RNA at different times and performed RNA-seq analysis. Time-course statistical analysis revealed hundreds of genes that changed expression by up to 550-fold...
November 9, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27820601/scai-promotes-dna-double-strand-break-repair-in-distinct-chromosomal-contexts
#2
Rebecca Kring Hansen, Andreas Mund, Sara Lund Poulsen, Maria Sandoval, Karolin Klement, Katerina Tsouroula, Maxim A X Tollenaere, Markus Räschle, Rebeca Soria, Stefan Offermanns, Thomas Worzfeld, Robert Grosse, Dominique T Brandt, Björn Rozell, Matthias Mann, Francesca Cole, Evi Soutoglou, Aaron A Goodarzi, Jeremy A Daniel, Niels Mailand, Simon Bekker-Jensen
DNA double-strand breaks (DSBs) are highly cytotoxic DNA lesions, whose accurate repair by non-homologous end-joining (NHEJ) or homologous recombination (HR) is crucial for genome integrity and is strongly influenced by the local chromatin environment. Here, we identify SCAI (suppressor of cancer cell invasion) as a 53BP1-interacting chromatin-associated protein that promotes the functionality of several DSB repair pathways in mammalian cells. SCAI undergoes prominent enrichment at DSB sites through dual mechanisms involving 53BP1-dependent recruitment to DSB-surrounding chromatin and 53BP1-independent accumulation at resected DSBs...
November 7, 2016: Nature Cell Biology
https://www.readbyqxmd.com/read/27760146/alignment-of-homologous-chromosomes-and-effective-repair-of-programmed-dna-double-strand-breaks-during-mouse-meiosis-require-the-minichromosome-maintenance-domain-containing-2-mcmdc2-protein
#3
Friederike Finsterbusch, Ramya Ravindranathan, Ihsan Dereli, Marcello Stanzione, Daniel Tränkner, Attila Tóth
Orderly chromosome segregation during the first meiotic division requires meiotic recombination to form crossovers between homologous chromosomes (homologues). Members of the minichromosome maintenance (MCM) helicase family have been implicated in meiotic recombination. In addition, they have roles in initiation of DNA replication, DNA mismatch repair and mitotic DNA double-strand break repair. Here, we addressed the function of MCMDC2, an atypical yet conserved MCM protein, whose function in vertebrates has not been reported...
October 2016: PLoS Genetics
https://www.readbyqxmd.com/read/27743378/rad51-regulates-chk1-stability-via-autophagy-to-promote-cell-growth-in-esophageal-squamous-carcinoma-cells
#4
Xinyi Zhu, Qiuhui Pan, Nan Huang, Jianchun Wu, Ni Zhen, Fenyong Sun, Zhi Li, Qingyuan Yang
The serine/threonine protein kinase CHK1 has been reported to bind to the recombinase RAD51 and facilitates its assembly in DNA damage sites via phosphorylation. However, the role of RAD51 in regulating the expression of CHK1 has never been explored. Here, we show that RAD51 is highly upregulated in esophageal squamous tumor tissues and its DMC1 domain significantly promotes cell growth of esophageal cancer (EC) cells through CHK1. To gain the mechanistic insights, firstly, in the presence of 3-methyladenine (3MA), an autophagy inhibitor, we found that the reduction of CHK1 and the inhibition of cell growth in RAD51-deficient EC109 cells were strikingly restored...
October 14, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/27739607/multiple-roles-of-foxj3-in-spermatogenesis-a-lesson-from-foxj3-conditional-knockout-mouse-models
#5
Lili Ni, Hongchang Xie, Li Tan
The transcription factor FOXJ3 (Forkhead box J3) is highly expressed in spermatogonia and meiotic spermatocytes within mouse testes. Here, we addressed how FOXJ3 might participate in spermatogenesis using two conditional knockout mouse models in which the Foxj3 was deleted from either spermatogonia or meiotic spermatocytes. Both models exhibited complete male sterility, but distinct etiologies: Deleting FOXJ3 from spermatogonia using Foxj3(flox/flox) , Mvh-Cre mice caused Sertoli-cell-only syndrome in males...
October 14, 2016: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/27678521/prevention-of-dna-rereplication-through-a-meiotic-recombination-checkpoint-response
#6
Nicole A Najor, Layne Weatherford, George S Brush
In the budding yeast Saccharomyces cerevisiae, unnatural stabilization of the cyclin-dependent kinase inhibitor Sic1 during meiosis can trigger extra rounds of DNA replication. When programmed DNA double-strand breaks are generated but not repaired due to absence of DMC1, a pathway involving the checkpoint gene RAD17 prevents this DNA rereplication. Further genetic analysis has now revealed that prevention of DNA rereplication also requires MEC1, which encodes a protein kinase that serves as a central checkpoint regulator in several pathways including the meiotic recombination checkpoint response...
September 27, 2016: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/27492080/transcription-factor-zfp38-is-essential-for-meiosis-prophase-i-in-male-mice
#7
Zechen Yan, Dandan Fan, Qingjun Meng, Jinjian Yang, Wei Zhao, Fei Guo, Dongjian Song, Ruiming Guo, Ke Sun, Jiaxiang Wang
The production of haploid gametes by meiosis is a cornerstone of sexual reproduction and maintenance of genome integrity. Zfp38 mRNA is expressed in spermatocytes, indicating that transcription factor ZFP38 has the potential to regulate transcription during meiosis. In this study, we generated Zfp38 conditional knockout mice (Zfp38(flox/flox), Stra8-Cre, hereafter called Zfp38 cKO) and found that spermatogenesis did not progress beyond meiosis prophase I in Zfp38 cKO mice. Using a chromosomal spread technique, we observed that Zfp38 cKO spermatocytes exhibited a failure in chromosomal synapsis observed by SYCP1/SYCP3 double staining...
November 2016: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/27467592/cell-killing-mechanisms-and-impact-on-gene-expression-by-gemcitabine-and-212pb-trastuzumab-treatment-in-a-disseminated-i-p-tumor-model
#8
Kwon Joong Yong, Diane E Milenic, Kwamena E Baidoo, Martin W Brechbiel
In pre-clinical studies, combination therapy with gemcitabine and targeted radioimmunotherapy (RIT) using 212Pb-trastuzumab showed tremendous therapeutic potential in the LS-174T tumor xenograft model of disseminated intraperitoneal disease. To better understand the underlying molecular basis for the observed cell killing efficacy, gene expression profiling was performed after a 24 h exposure to 212Pb-trastuzumab upon gemcitabine (Gem) pre-treatment in this model. DNA damage response genes in tumors were quantified using a real time quantitative PCR array (qRT-PCR array) covering 84 genes...
2016: PloS One
https://www.readbyqxmd.com/read/27329041/hop2-and-sae3-are-required-for-dmc1-mediated-double-strand-break-repair-via-homolog-bias-during-meiosis
#9
Hong-Rae Cho, Yoon-Ju Kong, Soo-Gil Hong, Keun Pil Kim
During meiosis, exchange of DNA segments occurs between paired homologous chromosomes in order to produce recombinant chromosomes, helping to increase genetic diversity within a species. This genetic exchange process is tightly controlled by the eukaryotic RecA homologs Rad51 and Dmc1, which are involved in strand exchange of meiotic recombination, with Rad51 participating specifically in mitotic recombination. Meiotic recombination requires an interaction between homologous chromosomes to repair programmed double-strand breaks (DSBs)...
July 2016: Molecules and Cells
https://www.readbyqxmd.com/read/27234615/characterization-of-recombinase-dmc1b-and-its-functional-role-as-rad51-in-dna-damage-repair-in-giardia-duodenalis-trophozoites
#10
Ana Laura Torres-Huerta, Rosa María Martínez-Miguel, María Luisa Bazán-Tejeda, Rosa María Bermúdez-Cruz
Homologous recombination (HR) is a highly conserved pathway for the repair of chromosomes that harbor DNA double-stranded breaks (DSBs). The recombinase RAD51 plays a key role by catalyzing the pairing of homologous DNA molecules and the exchange of information between them. Two putative DMC1 homologs (DMC1A and DMC1B) have been identified in Giardia duodenalis. In terms of sequences, GdDMC1A and GdDMC1B bear all of the characteristic recombinase domains: DNA binding domains (helix-turn-helix motif, loops 1 and 2), an ATPcap and Walker A and B motifs associated with ATP binding and hydrolysis...
August 2016: Biochimie
https://www.readbyqxmd.com/read/27214570/identification-of-putative-mek1-substrates-during-meiosis-in-saccharomyces-cerevisiae-using-quantitative-phosphoproteomics
#11
Raymond T Suhandynata, Lihong Wan, Huilin Zhou, Nancy M Hollingsworth
Meiotic recombination plays a key role in sexual reproduction as it generates crossovers that, in combination with sister chromatid cohesion, physically connect homologous chromosomes, thereby promoting their proper segregation at the first meiotic division. Meiotic recombination is initiated by programmed double strand breaks (DSBs) catalyzed by the evolutionarily conserved, topoisomerase-like protein Spo11. Repair of these DSBs is highly regulated to create crossovers between homologs that are distributed throughout the genome...
2016: PloS One
https://www.readbyqxmd.com/read/27052786/chromatin-architecture-may-dictate-the-target-site-for-dmc1-but-not-for-rad51-during-homologous-pairing
#12
Wataru Kobayashi, Motoki Takaku, Shinichi Machida, Hiroaki Tachiwana, Kazumitsu Maehara, Yasuyuki Ohkawa, Hitoshi Kurumizaka
In eukaryotes, genomic DNA is compacted as chromatin, in which histones and DNA form the nucleosome as the basic unit. DMC1 and RAD51 are essential eukaryotic recombinases that mediate homologous chromosome pairing during homologous recombination. However, the means by which these two recombinases distinctly function in chromatin have remained elusive. Here we found that, in chromatin, the human DMC1-single-stranded DNA complex bypasses binding to the nucleosome, and preferentially promotes homologous pairing at the nucleosome-depleted regions...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26976601/brca2-regulates-dmc1-mediated-recombination-through-the-brc-repeats
#13
Juan S Martinez, Catharina von Nicolai, Taeho Kim, Åsa Ehlén, Alexander V Mazin, Stephen C Kowalczykowski, Aura Carreira
In somatic cells, BRCA2 is needed for RAD51-mediated homologous recombination. The meiosis-specific DNA strand exchange protein, DMC1, promotes the formation of DNA strand invasion products (joint molecules) between homologous molecules in a fashion similar to RAD51. BRCA2 interacts directly with both human RAD51 and DMC1; in the case of RAD51, this interaction results in stimulation of RAD51-promoted DNA strand exchange. However, for DMC1, little is known regarding the basis and functional consequences of its interaction with BRCA2...
March 29, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/26960731/osdmc1-is-not-required-for-homologous-pairing-in-rice-meiosis
#14
Hongjun Wang, Qing Hu, Ding Tang, Xiaofei Liu, Guijie Du, Yi Shen, Yafei Li, Zhukuan Cheng
Meiotic homologous recombination is pivotal to sexual reproduction. DMC1, a conserved recombinase, is involved in directing single-end invasion between interhomologs during meiotic recombination. In this study, we identified OsDMC1A and OsDMC1B, two closely related proteins in rice (Oryza sativa) with high sequence similarity to DMC1 proteins from other species. Analysis of Osdmc1a and Osdmc1b Tos17 insertion mutants indicated that these genes are functionally redundant. Immunolocalization analysis revealed OsDMC1 foci occurred at leptotene, which disappeared from late pachytene chromosomes in wild-type meiocytes...
May 2016: Plant Physiology
https://www.readbyqxmd.com/read/26945774/exon-skipping-creates-novel-splice-variants-of-dmc1-gene-in-ruminants
#15
S Ahlawat, M Chopra, L Jaiswal, R Sharma, R Arora, B Brahma, S V Lal, S De
Disrupted meiotic cDNA1 (DMC1) recombinase plays a pivotal role in homology search and strand exchange reactions during meiotic homologous recombination. In the present study, full length coding sequence of DMC1 gene was sequence characterized for the first time from four ruminant species (cattle, buffalo, sheep and goat) and phylogenetic relationship of ruminant DMC1 with other eukaryotes was analyzed. DMC1 gene encodes a putative protein of 340 amino acids in cattle, sheep and buffalo and 341 amino acids in goat...
April 2016: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/26869299/sohlh2-is-essential-for-synaptonemal-complex-formation-during-spermatogenesis-in-early-postnatal-mouse-testes
#16
Miree Park, Youngeun Lee, Hoon Jang, Ok-Hee Lee, Sung-Won Park, Jae-Hwan Kim, Kwonho Hong, Hyuk Song, Se-Pill Park, Yun-Yong Park, Jung Jae Ko, Youngsok Choi
Spermatogenesis- and oogenesis-specific helix-loop-helix transcription factor 2 (SOHLH2) is exclusively expressed in germ cells of the gonads. Previous studies show that SOHLH2 is critical for spermatogenesis in mouse. However, the regulatory mechanism of SOHLH2 during early spermatogenesis is poorly understood. In the present study, we analyzed the gene expression profile of the Sohlh2-deficient testis and examined the role of SOHLH2 during spermatogenesis. We found 513 genes increased in abundance, while 492 genes decreased in abundance in 14-day-old Sohlh2-deficient mouse testes compared to wildtype mice...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26799944/ameliorative-effect-of-grape-seed-proanthocyanidin-extract-on-cadmium-induced-meiosis-inhibition-during-oogenesis-in-chicken-embryos
#17
Fuyin Hou, Min Xiao, Jian Li, Devin W Cook, Weidong Zeng, Caiqiao Zhang, Yuling Mi
Cadmium (Cd) is an environmental endocrine disruptor that has toxic effects on the female reproductive system. Here the ameliorative effect of grape seed proanthocyanidin extract (GSPE) on Cd-induced meiosis inhibition during oogenesis was explored. As compared with controls, chicken embryos exposed to Cd (3 µg/egg) displayed a changed oocyte morphology, decreased number of meiotic germ cells, and decreased expression of the meiotic marker protein γH2AX. Real time RT-PCR also revealed a significant down-regulation in the mRNA expressions of various meiosis-specific markers (Stra8, Spo11, Scp3, and Dmc1) together with those of Raldh2, a retinoic acid (RA) synthetase, and of the receptors (RARα and RARβ)...
April 2016: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/26709229/tolerance-of-dna-mismatches-in-dmc1-recombinase-mediated-dna-strand-exchange
#18
María V Borgogno, Mariela R Monti, Weixing Zhao, Patrick Sung, Carlos E Argaraña, Roberto J Pezza
Recombination between homologous chromosomes is required for the faithful meiotic segregation of chromosomes and leads to the generation of genetic diversity. The conserved meiosis-specific Dmc1 recombinase catalyzes homologous recombination triggered by DNA double strand breaks through the exchange of parental DNA sequences. Although providing an efficient rate of DNA strand exchange between polymorphic alleles, Dmc1 must also guard against recombination between divergent sequences. How DNA mismatches affect Dmc1-mediated DNA strand exchange is not understood...
March 4, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26596988/recombination-between-the-mouse-y-chromosome-short-arm-and-an-additional-y-short-arm-derived-chromosomal-segment-attached-distal-to-the-x-chromosome-par
#19
REVIEW
Fanny Decarpentrie, Obah A Ojarikre, Michael J Mitchell, Paul S Burgoyne
In a male mouse, meiosis markers of processed DNA double strand breaks (DSBs) such as DMC1 and RAD51 are regularly seen in the non-PAR region of the X chromosome; these disappear late in prophase prior to entry into the first meiotic metaphase. Marker evidence for DSBs occurring in the non-PAR region of the Y chromosome is limited. Nevertheless, historically it has been documented that recombination can occur within the mouse Y short arm (Yp) when an additional Yp segment is attached distal to the X and/or the Y pseudoautosomal region (PAR)...
June 2016: Chromosoma
https://www.readbyqxmd.com/read/26459995/structure-function-relationships-in-reca-protein-mediated-homology-recognition-and-strand-exchange
#20
REVIEW
Mara Prentiss, Chantal Prévost, Claudia Danilowicz
RecA family proteins include RecA, Rad51, and Dmc1. These recombinases are responsible for homology search and strand exchange. Homology search and strand exchange occur during double-strand break repair and in eukaryotes during meiotic recombination. In bacteria, homology search begins when RecA binds an initiating single-stranded DNA (ssDNA) in the primary DNA-binding site to form the presynaptic filament. The filament is a right-handed helix, where the initiating strand is bound deep within the filament...
2015: Critical Reviews in Biochemistry and Molecular Biology
keyword
keyword
73422
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"