Rania Ghouil, Simona Miron, Koichi Sato, Dejan Ristic, Sari E van Rossum-Fikkert, Pierre Legrand, Malika Ouldali, Jean-Marie Winter, Virginie Ropars, Gabriel David, Ana-Andreea Arteni, Claire Wyman, Puck Knipscheer, Roland Kanaar, Alex N Zelensky, Sophie Zinn-Justin
In meiotic homologous recombination (HR), BRCA2 facilitates loading of the recombinases RAD51 and DMC1 at the sites of double-strand breaks (DSBs). The HSF2BP-BRME1 complex interacts with BRCA2. Its absence causes a severe reduction in recombinase loading at meiotic DSB. We previously showed that, in somatic cancer cells ectopically producing HSF2BP, DNA damage can trigger HSF2BP-dependent degradation of BRCA2, which prevents HR. Here, we report that, upon binding to BRCA2, HSF2BP forms octameric rings that are able to interlock into a large ring-shaped 24-nucleotide oligomer...
October 27, 2023: Science Advances