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https://www.readbyqxmd.com/read/28735760/first-characterization-of-immunogenic-conjugates-of-vi-negative-salmonella-typhi-o-specific-polysaccharides-with-repa-protein-for-vaccine-development
#1
M Salman, F St Michael, A Ali, A Jabbar, C Cairns, A C Hayes, M Rahman, M Iqbal, A Haque, A D Cox
Efficacious typhoid vaccines for young children will significantly reduce the disease burden in developing world. The Vi polysaccharide based conjugate vaccines (Vi-rEPA) against Salmonella Typhi Vi positive strains has shown high efficacy but may be ineffective against Vi negative S. Typhi. In this study, for the first time, we report the synthesis and evaluation of polysaccharide-protein conjugates of Vi negative S. Typhi as potential vaccine candidates. Four different conjugates were synthesized using recombinant exoprotein A of Pseudomonas aeruginosa (rEPA) and human serum albumin (HSA) as the carrier proteins, using either direct reductive amination or an intermediate linker molecule, adipic acid dihydrazide (ADH)...
July 21, 2017: Journal of Immunological Methods
https://www.readbyqxmd.com/read/28648453/tolerance-of-pseudomonas-aeruginosa-in-in-vitro-biofilms-to-high-level-peracetic-acid-disinfection
#2
A B Akinbobola, L Sherry, W G Mckay, G Ramage, C Williams
BACKGROUND: Biofilm has been suggested as a cause of disinfection failures in flexible endoscopes where no lapses in the decontamination procedure can be identified. To test this theory, the activity of peracetic acid, one of the widely used disinfectants in the reprocessing of flexible endoscopes, was evaluated against both planktonic and sessile communities of Pseudomonas aeruginosa. AIM: To investigate the ability of P. aeruginosa biofilm to survive high-level peracetic acid disinfection...
June 23, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28630071/albumin-inhibits-pseudomonas-aeruginosa-quorum-sensing-and-alters-polymicrobial-interactions
#3
Allie Clinton Smith, Anne Rice, Bryan Sutton, Rebecca Gabrilska, Aimee K Wessel, Marvin Whiteley, Kendra P Rumbaugh
Polymicrobial interactions are complex and can influence the course of an infection, as is the case when two or more species exhibit a synergism that produces a disease state not seen with any of the individual species alone. Cell-to-cell signaling is key to many of these interactions, but little is understood about how the host environment influences polymicrobial interactions or signaling between bacteria. Chronic wounds are typically polymicrobial, with Staphylococcus aureus and Pseudomonas aeruginosa being the two most commonly isolated species...
June 19, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28595608/antimicrobial-activity-toxicity-and-anti-inflammatory-potential-of-methanolic-extracts-of-four-ethnomedicinal-plant-species-from-punjab-pakistan
#4
Rabia Naz, Hafsa Ayub, Sajid Nawaz, Zia Ul Islam, Tayyaba Yasmin, Asghari Bano, Abdul Wakeel, Saqib Zia, Thomas H Roberts
BACKGROUND: The plant species Aristolochia indica (AI), Melilotus indicus (MI), Tribulus terrestris (TT) and Cuscuta pedicellata (CP) are widely used in folk medicine in the villages around Chowk Azam, South Punjab, Pakistan. The aim of this study was to evaluate the antioxidant activity, phytochemical composition, and the antibacterial, antifungal, cytotoxic and anti-inflammatory potential of the four medicinal plants listed above. For CP stem, this study represents (to the best of our knowledge) the first time phytochemicals have been identified and the antioxidant and anti-inflammatory potential determined...
June 8, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28448954/copper-complexes-containing-thiosemicarbazones-derived-from-6-nitropiperonal-antimicrobial-and-biophysical-properties
#5
Floyd A Beckford, Kelsey R Webb
A series of four thiosemicarbazones from 6-nitropiperonal along with the corresponding copper complexes were synthesized. The biophysical characteristics of the complexes were investigated by the binding to DNA and human serum albumin. The binding to DNA is moderate; the binding constants run from (0.49-7.50)×10(4)M(-1). In relation to HSA, the complexes interact strongly with binding constants on the order of 10(5)M(-1). The complexes also display antioxidant behavior as determined by the ability to scavenge diphenylpicrylhydrazyl (dpph) and nitric oxide radicals...
April 20, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28289362/study-on-biofilm-formation-in-burn-wound-infection-in-a-pediatric-hospital-in-chennai-india
#6
M Ramakrishnan, S Putli Bai, M Babu
Infection is one of the major causes of death in pediatric burns in India. This work was conducted in an exclusive Children's Hospital (KKCTH) with a total of 220 beds, of which ten beds in the burn unit and two isolation beds in the 28-bed PICU are for burns patients (more than 20% TBSA burns) with sepsis. In this study, 30 burn wound swab isolates obtained from 14 pediatric burns patients (admitted to the burns ward and transferred to PICU) from November 2013 to March 2014 were investigated. Cultures were done on the first day for all patients and empirical antibiotic administration was started for those with septic burns (14 in total) with piperacillin-tazobactam and vancomycin...
December 31, 2016: Annals of Burns and Fire Disasters
https://www.readbyqxmd.com/read/28155216/evaluation-of-the-role-of-substrate-and-albumin-on-pseudomonas-aeruginosa-biofilm-morphology-through-fesem-and-ftir-studies-on-polymeric-biomaterials
#7
S Dutta Sinha, Susmita Chatterjee, P K Maiti, S Tarafdar, S P Moulik
Bacterial biofilms pose the greatest challenge to implant surgeries leading to device-related infections and implant failure. Our present study aims at monitoring the variation in the biofilm architecture of a clinically isolated strain and ATCC 27853 strain of Pseudomonas aeruginosa on two polymeric biomaterials, used in implants. The perspective of our study is to recognize the potential of these two biomaterials to create biofilm infections and develop the understanding regarding their limitations of use and handle patients with this deeper insight...
May 2017: Progress in biomaterials
https://www.readbyqxmd.com/read/27932683/survival-of-bacteria-of-laboratory-animal-origin-on-cage-bedding-and-inactivation-by-hydrogen-peroxide-vapour
#8
Laurentiu Benga, W Peter M Benten, Eva Engelhardt, Christina Gougoula, Roland Schulze-Röbbecke, Martin Sager
This study aims to determine the ability of laboratory animal bacteria to resist desiccation and inactivation by hydrogen peroxide vapour (HPV) on paper bedding pieces. Bedding pieces were saturated with bacterial suspensions in water or 2% (w/v) bovine serum albumin (BSA) in water, and held in a mouse facility. Viable counts showed variable survival rates over time for the bacterial species used ([Pasteurella] pneumotropica, Muribacter muris, Pseudomonas aeruginosa, Acinetobacter redioresistens, Escherichia coli, Klebsiella oxytoca, Bordetella bronchiseptica, Bordetella hinzii, Enterococcus faecalis, β-haemolytic Streptococcus spp...
December 8, 2016: Laboratory Animals
https://www.readbyqxmd.com/read/27796875/molecular-and-spectroscopic-characterization-of-aspergillus-flavipes-and-pseudomonas-putida-l-methionine-%C3%AE-lyase-in-vitro
#9
Ashraf S A El-Sayed, Laura E Ruff, Salah E Abdel Ghany, Gul Shad Ali, Sadik Esener
Pseudomonas putida L-methionine γ-lyase (PpMGL) has been recognized as an efficient anticancer agent, however, its antigenicity and stability remain as critical challenges for its clinical use. From our studies, Aspergillus flavipes L-methionine γ-lyase (AfMGL) displayed more affordable biochemical properties than PpMGL. Thus, the objective of this work was to comparatively assess the functional properties of AfMGL and PpMGL via stability of their internal aldimine linkage, tautomerism of pyridoxal 5'-phosphate (PLP) and structural stability responsive to physicochemical factors...
April 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/27711156/in-vitro-antibacterial-activity-of-rhodanine-derivatives-against-pathogenic-clinical-isolates
#10
Ahmed AbdelKhalek, Charles R Ashby, Bhargav A Patel, Tanaji T Talele, Mohamed N Seleem
Bacterial infections present a serious challenge to healthcare practitioners due to the emergence of resistance to numerous conventional antibacterial drugs. Therefore, new bacterial targets and new antimicrobials are unmet medical needs. Rhodanine derivatives have been shown to possess potent antimicrobial activity via a novel mechanism. However, their potential use as antibacterials has not been fully examined. In this study, we determined the spectrum of activity of seven rhodanine derivatives (compounds Rh 1-7) against clinical isolates of Gram-positive and Gram-negative bacterial strains and Candida albicans...
2016: PloS One
https://www.readbyqxmd.com/read/27680092/a-2s-albumin-from-the-seed-cake-of-ricinus-communis-inhibits-trypsin-and-has-strong-antibacterial-activity-against-human-pathogenic-bacteria
#11
Pedro F N Souza, Ilka M Vasconcelos, Fredy D A Silva, Frederico B Moreno, Ana C O Monteiro-Moreira, Luciana M R Alencar, Angélica S G Abreu, Jeanlex S Sousa, José T A Oliveira
Hospital-acquired infections caused by antibiotic-resistant bacteria threaten the lives of many citizens all over the world. Discovery of new agents to hinder bacterial development would have a significant impact on the treatment of infections. Here, the purification and characterization of Rc-2S-Alb, a protein that belongs to the 2S albumin family, from Ricinus communis seed cake, are reported. Rc-2S-Alb was purified after protein extraction with Tris-HCl buffer, pH 7.5, fractionation by ammonium sulfate (50-75%), and chromatography on Phenyl-Sepharose and DEAE-Sepharose...
October 28, 2016: Journal of Natural Products
https://www.readbyqxmd.com/read/27581112/treatment-failure-and-leg-amputation-among-patients-with-foot-osteomyelitis
#12
Neal R Barshes, Cezarina Mindru, Chester Ashong, Maria Rodriguez-Barradas, Barbara W Trautner
We sought to identify factors associated with treatment failure and leg amputations among those patients who presented with foot osteomyelitis. Characteristics, treatments, and outcomes for all patients treated for probable or definite foot osteomyelitis (per consensus definition) between January 2011 and March 2015 were reviewed. Multivariate Cox regression models were used to identify risk factors for treatment failure (unanticipated resection of additional bone or leg amputation) and of leg amputation alone...
December 2016: International Journal of Lower Extremity Wounds
https://www.readbyqxmd.com/read/27573289/influence-of-molecular-design-on-biodistribution-and-targeting-properties-of-an-affibody-fused-her2-recognising-anticancer-toxin
#13
Mohamed Altai, Hao Liu, Anna Orlova, Vladimir Tolmachev, Torbjörn Gräslund
Targeted delivery of toxins is a promising way to treat disseminated cancer. The use of monoclonal antibodies as targeting moiety has provided proof-of-principle for this approach. However, extravasation and tissue penetration rates of antibody-based immunotoxins are limited due to antibody bulkiness. The use of a novel class of targeting probes, Affibody molecules, provides smaller toxin-conjugated constructs, which may improve targeting. Earlier, we have demonstrated that affitoxins containing a HER2-targeting Affibody moiety and a deimmunized and truncated exotoxin A from Pseudomonas aeruginosa, PE38X8, provide highly selective toxicity to HER2-expressing cancer cells...
September 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27426954/characterization-of-aerobic-oil-and-grease-degrading-bacteria-in-wastewater
#14
Alexis Nzila, Assad Thukair, Saravanan Sankara, Shaikh Abdur Razzak
A bacterial consortium that degrades cooking oil (CO) has been isolated in wastewater (WW) samples, by enrichment in olive CO. This consortium could degrade 90% of CO within 7-9 days (from an initial 1% [w/v]), and it is more active at alkaline conditions. The 16S ribonucleic acid (RNA) gene analysis showed that it contains five bacterium species: Stenotrophomonas rhizophila, Sphingobacterium sp., Pseudomonas libanensis, Pseudomonas poae and Pseudomonas aeruginosa. This consortium can degrade the free fatty acids (FFA): palmitic, stearic, oleic, linoleic and linolenic acids; glycerol, glucose and amylose; and albumin, but could not efficiently degrade carboxymethyl-cellulose...
March 2017: Environmental Technology
https://www.readbyqxmd.com/read/27381280/bioactive-and-biocompatible-copper-containing-glass-ceramics-with-remarkable-antibacterial-properties-and-high-cell-viability-designed-for-future-in-vivo-trials
#15
R A Popescu, K Magyari, A Vulpoi, D L Trandafir, E Licarete, M Todea, R Ştefan, C Voica, D C Vodnar, S Simon, I Papuc, L Baia
In the present study our interest is focused on finding the efficiency of 60SiO2·(32 - x)CaO·8P2O5·xCuO (mol%) glass-ceramics, with 0 ≤ x ≤ 4 mol%, in terms of bioactivity, biocompatibility, antibacterial properties and cell viability in order to determine the most appropriate composition for their further use in in vivo trials. The sol-gel synthesized samples show a preponderantly amorphous structure with a few crystallization centers associated with the formation of an apatite and calcium carbonate crystalline phases...
July 19, 2016: Biomaterials Science
https://www.readbyqxmd.com/read/27208443/surface-modification-with-zwitterionic-cysteine-betaine-for-nanoshell-assisted-near-infrared-plasmonic-hyperthermia
#16
Chun-Jen Huang, Sz-Hau Chu, Chien-Hung Li, T Randall Lee
Nanoparticles decorated with biocompatible coatings have received considerable attention in recent years for their potential biomedical applications. However, the desirable properties of nanoparticles for in vivo uses, such as colloidal stability, biodistribution, and pharmacokinetics, require further research. In this work, we report a bio-derived zwitterionic surface ligand, cysteine betaine (Cys-b) for the modification of hollow gold-silver nanoshells, giving rise to hyperthermia applications. Cys-b coatings on planar substrates and nanoshells were compared to conventional (11-mercaptoundecyl)tri(ethylene glycol) (OEG-thiol) to investigate their effects on the fouling resistance, colloidal stability, environmental tolerance, and photothermal properties...
September 1, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27045418/the-distribution-and-resistance-of-pathogens-among-solid-organ-transplant-recipients-with-pseudomonas-aeruginosa-infections
#17
Aijing Luo, Zhuqing Zhong, Qiquan Wan, Qifa Ye
BACKGROUND: Pseudomonas aeruginosa infection remains a life-threatening complication after solid organ transplantation (SOT). We aimed to investigate the distribution and drug susceptibility of pathogens, and clinical characteristics of SOT recipients with Pseudomonas aeruginosa infections. MATERIAL/METHODS: A total of 55 SOT recipients who developed 61 episodes of Pseudomonas aeruginosa infections between January 1, 2003 and July 31, 2015 were retrospectively analyzed...
April 5, 2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/26822870/potential-biological-efficacy-of-pinus-plant-species-against-oxidative-inflammatory-and-microbial-disorders
#18
Aditi Sharma, Rohit Goyal, Lalit Sharma
BACKGROUND: Traditionally, Pine has been used to treat oxidative and inflammatory disorders. The study was aimed to investigate biological potential of phytoconstituents of Pinus plant species: Pinus roxburghii, Pinus wallichiana and Pinus gerardiana using in-vitro antioxidant, anti-inflammatory and antimicrobial methods. METHOD: The hydro-alcoholic extraction of dried plant: stem bark was done and the antioxidant activity was evaluated using free radical scavenging methods for 1,1-diphenyl-2-picrylhydrazyl, (DPPH), nitric oxide and hydrogen peroxide radicals, reducing power assays, and total antioxidant capacity...
January 28, 2016: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/26678838/eprs-an-autotransporter-serine-protease-plays-an-important-role-in-various-pathogenic-phenotypes-of-pseudomonas-aeruginosa
#19
Y Kida, J Taira, K Kuwano
Pseudomonas aeruginosa possesses an arsenal of both cell-associated (flagella, pili, alginate, etc.) and extracellular (exotoxin A, proteases, type III secretion effectors, etc.) virulence factors. Among them, secreted proteases that damage host tissues are considered to play an important role in the pathogenesis of P. aeruginosa infections. We previously reported that EprS, an autotransporter protease of P. aeruginosa, induces host inflammatory responses through protease-activated receptors. However, little is known about the role of EprS as a virulence factor of P...
February 2016: Microbiology
https://www.readbyqxmd.com/read/26452549/effect-of-treatment-with-an-overheated-dry-saturated-steam-vapour-disinfection-system-on-multidrug-and-extensively-drug-resistant-nosocomial-pathogens-and-comparison-with-sodium-hypochlorite-activity
#20
COMPARATIVE STUDY
Maria Bagattini, Raffaella Buonocore, Maria Giannouli, Dario Mattiacci, Rossella Bellopede, Nicola Grimaldi, Antonio Nardone, Raffaele Zarrilli, Maria Triassi
BACKGROUND: The development of portable steam generators has made disinfection of the environment more practical. This study assessed the "in vitro" ability of an overheated dry-saturated steam vapour system to kill multidrug and extensively-drug resistant nosocomial pathogens, defining the antimicrobial spectrum and the contact times compared with the activity of sodium hypochlorite. METHODS: The antibacterial efficacy of the overheated dry-saturated steam vapour system and of sodium hypochlorite against nosocomial pathogen isolates: extensively drug-resistant Acinetobacter baumannii, Pseudomonas aeruginosa, carbapenemase-producing Klebsiella pneumoniae, methicillin-resistant Staphylococcus aureus, high-level aminoglycoside-resistant Enterococcus faecalis, Candida parapsilosis and Aspergillus fumigatus were assessed using a surface time-kill test carried out on glass surfaces, with or without bovine serum albumin (BSA)...
2015: BMC Research Notes
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