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https://www.readbyqxmd.com/read/28642470/quantitative-proteomics-analysis-reveals-novel-targets-of-mir-21-in-zebrafish-embryos
#1
Ying Wu, Qi-Yong Lou, Feng Ge, Qian Xiong
MicroRNAs (miRNAs) are noncoding RNAs which control gene expression by the suppression of translation or the degradation of mRNAs. Dre-miR-21 (miR-21) has been reported to impact cardiac valvulogenesis in zebrafish embryos. However, the target genes of miR-21 are still largely unknown. Here a tandem isobaric mass tag (TMT)-based quantitative proteomic strategy was employed to identify the global profile of miR-21-regulated proteins. A total of 251 proteins were dysregulated after miR-21 knockdown, suggesting that they may be regulated by miR-21...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28629377/quantitative-phosphoproteome-on-the-silkworm-bombyx-mori-cells-infected-with-baculovirus
#2
Jauharotus Shobahah, Shengjie Xue, Dongbing Hu, Cui Zhao, Ming Wei, Yanping Quan, Wei Yu
BACKGROUND: Bombyx mori has become an important model organism for many fundamental studies. Bombyx mori nucleopolyhedrovirus (BmNPV) is a significant pathogen to Bombyx mori, yet also an efficient vector for recombinant protein production. A previous study indicated that acetylation plays many vital roles in several cellular processes of Bombyx mori while global phosphorylation pattern upon BmNPV infection remains elusive. METHOD: Employing tandem mass tag (TMT) labeling and phosphorylation affinity enrichment followed by high-resolution LC-MS/MS analysis and intensive bioinformatics analysis, the quantitative phosphoproteome in Bombyx mori cells infected by BmNPV at 24 hpi with an MOI of 10 was extensively examined...
June 19, 2017: Virology Journal
https://www.readbyqxmd.com/read/28627880/site-specific-fucosylation-analysis-identifies-glycoproteins-associated-with-aggressive-prostate-cancer-cell-lines-using-tandem-affinity-enrichments-of-intact-glycopeptides-followed-by-mass-spectrometry
#3
Jianliang Zhou, Weiming Yang, Yingwei Hu, Naseruddin Hoti, Yang Liu, Punit Shah, Shisheng Sun, David J Clark, Stefani N Thomas, Hui Zhang
Fucosylation (Fuc) of glycoproteins plays an important role in regulating protein function and has been associated with the development of several cancer types including prostate cancer (Pca). Therefore, the research of Fuc glycoproteins has attracted increasing attention recently in analytical field. Herein, a strategy based on lectin affinity enrichments of intact glycopeptides followed by mass spectrometry has been established to evaluate the specificities of various Fuc-binding lectins for glycosite-specific Fuc analysis of non-aggressive (NAG) and aggressive (AG) Pca cell lines...
June 19, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28622602/assessment-of-the-phenolic-profile-antimicrobial-activity-and-oxidative-stability-of-transgenic-perilla-frutescens-l-overexpressing-tocopherol-methyltransferase-%C3%AE-tmt-gene
#4
Bimal Kumar Ghimire, Chang Yeon Yu, Ill-Min Chung
This study evaluated the effects of enhanced concentrations of α-tocopherol and phenolic compounds on the resistance and stability of Perilla oil in transgenic Perilla frutescens plants against various tested pathogenic bacteria by over-expressing the γ-tmt gene. The concentration of phenolic compounds in the non-transgenic samples was 9313.198 ± 18.887 μg g(-1) dry weight (DW), whereas the total concentration of the transgenic samples ranged from 9118.015 ± 18.822 to 10527.612 ± 20.411 μg g(-1) DW...
June 7, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28620049/the-x-linked-deubiquitinase-usp9x-is-an-integral-component-of-centrosome
#5
Qian Wang, Yiman Tang, Yue Xu, Shilei Xu, Yong Jiang, Qiuping Dong, Yongsheng Zhou, Wenshu Ge
The X-linked deubiquitinase USP9X has been implicated in multiple pathological disorders including malignancies and X-linked intellectual disability. However, its biological function and substrate repertoire remain to be investigated. In this study, we utilized the TMT (tandem mass tags) labeling assay to identify USP9X regulated proteins, and revealed that the expression of multiple genes is altered in USP9X deficient cells. Interestingly, we showed that USP9X promotes stabilization of centrosome protein PCM1 and CEP55 through its catalytic activity...
June 15, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28615758/regional-cerebral-glucose-metabolism-and-its-association-with-phenotype-and-cognitive-functioning-in-patients-with-autism
#6
B N Anil Kumar, Savita Malhotra, Anish Bhattacharya, Sandeep Grover, Y K Batra
INTRODUCTION: In spite of three decades of neuroimaging, we are unable to find consistent and coherent anatomical or pathophysiological basis for autism as changes are subtle and there are no studies from India. AIM: To study the regional cerebral glucose metabolism in children with autism using positron emission tomography (PET) scan and to study the behavior and cognitive functioning among them. MATERIALS AND METHODS: Ten subjects (8-19 years) meeting Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition criteria for autism were evaluated on Childhood Autism Rating Scale (CARS), trail making test (TMT) A and B, Wisconsin card sorting test, Raven's progressive matrices, and PET scan...
May 2017: Indian Journal of Psychological Medicine
https://www.readbyqxmd.com/read/28613995/fear-of-self-annihilation-and-existential-uncertainty-as-predictors-of-worldview-defense-comparing-terror-management-and-uncertainty-theories
#7
Mark Rubin
Terror management theory (TMT) proposes that thoughts of death trigger a concern about self-annihilation that motivates the defense of cultural worldviews. In contrast, uncertainty theorists propose that thoughts of death trigger feelings of uncertainty that motivate worldview defense. University students (N = 414) completed measures of the chronic fear of self-annihilation and existential uncertainty as well as the need for closure. They then evaluated either a meaning threat stimulus or a control stimulus...
June 14, 2017: Journal of Social Psychology
https://www.readbyqxmd.com/read/28610687/assessing-the-thickness-of-the-vaginal-wall-and-vaginal-mucosa-in-pre-menopausal-versus-post-menopausal-women-by-transabdominal-ultrasound-a-feasibility-study
#8
Adrian Balica, Daniella Wald-Spielman, Katherine Schertz, Susan Egan, Gloria Bachmann
OBJECTIVE: As life expectancy increases, the number of women reporting adverse genito-urinary symptoms (genitourinary syndrome of menopause; GSM) from menopause, including vaginal dryness and sexual pain, also will increase. Current objective measurements of vaginal atrophy such as maturation index require vaginal swabs and are invasive; at present, no minimally invasive measurements exist. The purpose of this study was to assess whether total vaginal wall thickness (TVT) and total vaginal mucosa thickness (TMT) as measured by transabdominal ultrasound could qualify as additional objective markers of vaginal wall thinning which could be related to menopausal status...
August 2017: Maturitas
https://www.readbyqxmd.com/read/28609148/an-intensive-outpatient-treatment-program-for-combat-related-ptsd-trauma-management-therapy
#9
Deborah C Beidel, Jeremy W Stout, Sandra M Neer, B Christopher Frueh, Carl Lejuez
An estimated 18.5% of veterans returning from Operation Iraqi Freedom, Operation Enduring Freedom, and Operation New Dawn are suffering from posttraumatic stress or posttraumatic stress disorder (PTSD). The number of veterans and service duty personnel requesting VA health care services is increasing, and the VA's ability to handle requests is dwindling. Thus, both the content of interventions and the format by which these services are delivered need to be reconceptualized. The authors present a multicomponent intervention program known as Trauma Management Therapy (TMT), a comprehensive, empirically supported treatment that can be delivered in an intensive, 3-week outpatient format...
2017: Bulletin of the Menninger Clinic
https://www.readbyqxmd.com/read/28601572/corpus-callosum-atrophy-as-a-marker-of-clinically-meaningful-cognitive-decline-in-secondary-progressive-multiple-sclerosis-impact-on-employment-status
#10
Athanasios Papathanasiou, Lambros Messinis, Petros Zampakis, Panagiotis Papathanasopoulos
Cognitive impairment in Multiple Sclerosis (MS) is more frequent and pronounced in secondary progressive MS (SPMS). Cognitive decline is an important predictor of employment status in patients with MS. Magnetic Resonance Imaging (MRI) markers have been used to associate tissue damage with cognitive dysfunction. The aim of the study was to designate the MRI marker that predicts cognitive decline in SPMS and explore its effect on employment status. 30 SPMS patients and 30 healthy participants underwent neuropsychological assessment using the Trail Making Test (TMT) parts A and B, semantic and phonological verbal fluency task and a computerized cognitive screening battery (Central Nervous System Vital Signs)...
June 7, 2017: Journal of Clinical Neuroscience: Official Journal of the Neurosurgical Society of Australasia
https://www.readbyqxmd.com/read/28599071/predator-odor-evokes-sex-independent-stress-responses-in-male-and-female-wistar-rats-and-reduces-phosphorylation-of-cyclic-adenosine-monophosphate-response-element-binding-protein-creb-in-the-male-but-not-the-female-hippocampus
#11
Damek Homiack, Emma O'Cinneide, Sema Hajmurad, Brett Barrileaux, Mary Stanley, Michael R Kreutz, Laura Schrader
Post-traumatic stress disorder (PTSD) is characterized by memory disturbances following trauma. Acute predator threat has emerged as an ethological model of PTSD, yet the effects of predator odor on signaling cascades associated with long-term memory remain poorly understood. In this study, we exposed male and female Wistar rats to the synthetic predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT) to assess behavioral and physiological responses as well as rapid modulation of signal transduction cascades associated with learning and memory in the male and female hippocampus...
June 9, 2017: Hippocampus
https://www.readbyqxmd.com/read/28596126/reaction-time-processing-speed-and-sustained-attention-in-schizophrenia-impact-on-social-functioning
#12
Guillermo Lahera, Alicia Ruiz, Antía Brañas, María Vicens, Arantxa Orozco
INTRODUCTION: Previous studies have linked processing speed with social cognition and functioning of patients with schizophrenia. A discriminant analysis is needed to determine the different components of this neuropsychological construct. This paper analyzes the impact of processing speed, reaction time and sustained attention on social functioning. MATERIAL AND METHODS: 98 outpatients between 18 and 65 with DSM-5 diagnosis of schizophrenia, with a period of 3 months of clinical stability, were recruited...
June 5, 2017: Revista de Psiquiatrí́a y Salud Mental
https://www.readbyqxmd.com/read/28592136/relationship-of-depression-with-executive-functions-and-visuospatial-memory-in-elderly
#13
Monika Klojčnik, Voyko Kavcic, Karin Bakracevic Vukman
Cognitive deficits are a potential part of the clinical picture of depression, especially when it comes to late-life depression. The present study was carried out to establish whether distinctive cognitive deficiencies can be linked with depression in the late-life period, especially in executive functioning, working memory, and visuospatial memory. Our sample consisted of 71 seniors in the age range between 69 and 85 years. A battery of neurocognitive tests was used, including tests of executive functioning (Trail Making Test [TMT], part B, Stroop color word test, semantic word fluency test, and partially Rey-Osterrieth Complex Figure test [ROCF]), tests of attention and working memory (TMT, part A and digit span), and test of visuospatil ability and memory (ROCF)...
January 1, 2017: International Journal of Aging & Human Development
https://www.readbyqxmd.com/read/28574939/management-of-tarsometatarsal-joint-injuries
#14
Brian M Weatherford, John G Anderson, Donald R Bohay
Joint disruptions to the tarsometatarsal (TMT) joint complex, also known as the Lisfranc joint, represent a broad spectrum of pathology from subtle athletic sprains to severe crush injuries. Although injuries to the TMT joint complex are uncommon, when missed, they may lead to pain and dysfunction secondary to posttraumatic arthritis and arch collapse. An understanding of the appropriate anatomy, mechanism, physical examination, and imaging techniques is necessary to diagnose and treat injuries of the TMT joints...
June 1, 2017: Journal of the American Academy of Orthopaedic Surgeons
https://www.readbyqxmd.com/read/28573139/analysis-of-differentially-expressed-proteins-in-mycobacterium-avium-infected-macrophages-comparing-with-mycobacterium-tuberculosis-infected-macrophages
#15
Dongjun Yang, Xin Fu, Shiyi He, Xueping Ning, Min Ling
Mycobacterium avium (MA) belongs to the intracellular parasitic bacteria. To better understand how MA survives within macrophages and the different pathogenic mechanisms of MA and Mycobacterium tuberculosis (MTB), tandem mass tag (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis have been used to determine the proteins which are differentially expressed in MA-infected and MTB-infected macrophages. 369 proteins were found to be differentially expressed in MA-infected cells but not in MTB-infected cells...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28566637/2-4-bis-1-1-dimethylethyl-phenol-from-cinnamomum-loureirii-improves-cognitive-deficit-cholinergic-dysfunction-and-oxidative-damage-in-tmt-treated-mice
#16
Cho Rong Kim, Soo Jung Choi, Jae Kyeom Kim, Chan Kyu Park, Min Chul Gim, Youn-Jung Kim, Gwi Gun Park, Dong-Hoon Shin
We previously reported that the extract of Cinnamomum loureirii (C. loureirii) significantly inhibited acetylcholinesterase (AChE), and identified 2,4-bis(1,1-dimethylethyl)phenol (BP) from C. loureirii as a potential AChE inhibitor. The present study, therefore was undertaken to demonstrate the effects of BP from C. loureirii on learning and memory impairment in trimethyltin (TMT)-treated ICR mice. Y-maze and passive avoidance tests were used to test cognitive ability. Further, changes in biochemical parameters in the brain tissue were also assessed in response to TMT injection and BP intervention...
2017: Biological & Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/28559245/temporal-transcriptomic-and-proteomic-landscapes-of-deteriorating-pancreatic-islets-in-type-2-diabetic-rats
#17
Junjie Hou, Zonghong Li, Wen Zhong, Qiang Hao, Lei Lei, Linlin Wang, Dongyu Zhao, Pingyong Xu, Yifa Zhou, You Wang, Tao Xu
Progressive reduction in β-cell mass and function comprise the core of the pathogenesis mechanism of type 2 diabetes (T2D). The process of deteriorating pancreatic islets, in which a complex network of molecular events was involved, is not yet fully characterized. Here we employed RNA Sequencing and tandem mass tag (TMT) based quantitative proteomics technology to measure the temporal mRNA and protein expression changes of pancreatic islets in Goto-Kakizaki (GK) rats from 4 to 24 weeks of age. Our omics dataset outlined the dynamics of molecular network during the deterioration of GK islets as two stages: the early stage (4-6 weeks) is characterized by anaerobic glycolysis, inflammation priming, and compensation for insulin synthesis, whereas the late stage (8-24 weeks) is characterized by inflammation amplification and compensation failure...
May 30, 2017: Diabetes
https://www.readbyqxmd.com/read/28545005/the-efficacy-of-trauma-management-therapy-a-controlled-pilot-investigation-of-a-three-week-intensive-outpatient-program-for-combat-related-ptsd
#18
Deborah C Beidel, B Christopher Frueh, Sandra M Neer, Carl W Lejuez
Despite the 8-18.5% of returning Operation Iraqi Freedom (OIF), Operation Enduring Freedom (OEF) and Operation New Dawn (OND) veterans who are suffering from posttraumatic stress disorder (PTSD), few receive empirically supported treatments. Among those that do, the dropout rate is high and more than 50% retain their diagnosis after treatment. This study evaluated the efficacy of Trauma Management Therapy (TMT), delivered in a 3-week intensive outpatient (IOP) format. TMT combines virtual-reality augmented individual exposure therapy with a group intervention to address social isolation, anger, and depression...
May 11, 2017: Journal of Anxiety Disorders
https://www.readbyqxmd.com/read/28539952/executive-dysfunction-in-obsessive-compulsive-disorder-and-anterior-cingulate-based-resting-state-functional-connectivity
#19
Je-Yeon Yun, Joon Hwan Jang, Wi Hoon Jung, Na Young Shin, Sung Nyun Kim, Jae Yeon Hwang, Jun Soo Kwon
OBJECTIVE: Executive dysfunction might be an important determinant for response to pharmacotherapy in obsessive-compulsive disorder (OCD), and could be sustained independently of symptom relief. The anterior cingulate cortex (ACC) has been indicated as a potential neural correlate of executive functioning in OCD. The present study examined the brain-executive function relationships in OCD from the ACC-based resting state functional connectivity networks (rs-FCNs), which reflect information processing mechanisms during task performance...
May 2017: Psychiatry Investigation
https://www.readbyqxmd.com/read/28534617/comparative-proteomics-reveals-timely-transport-into-cilia-of-regulators-or-effectors-as-a-mechanism-underlying-ciliary-disassembly
#20
Limei Wang, Lixiao Gu, Dan Meng, Qiong Wu, Haiteng Deng, Junmin Pan
Primary cilia are assembled and disassembled during cell cycle progression. During ciliary disassembly, ciliary axonemal microtubules (MTs) are depolymerized accompanied with extensive posttranslational protein modifications of ciliary proteins including protein phosphorylation, methylation and ubiquitination. These events are hypothesized to involve transport of effectors or regulators into cilia at the time of ciliary disassembly from the cell body. To prove this hypothesis and identify new proteins involved in ciliary disassembly, we analyzed disassembling flagella in Chlamydomonas using comparative proteomics with TMT labeling...
May 23, 2017: Journal of Proteome Research
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