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https://www.readbyqxmd.com/read/28823095/methanobrevibacter-smithii-a-methanogen-consistently-colonising-the-newborn-stomach
#1
G Grine, M A Boualam, M Drancourt
Methanobrevibacter smithii is the main human methanogen almost always found in the digestive tract of adults. Yet, the age at which M. smithii establishes itself as part of the developing intestinal microflora remains unknown. In order to gain insight into this, we developed a polyphasic approach, including microscopic observation by fluorescence in situ hybridisation, polymerase chain reaction (PCR) sequencing detection, identification and culture, to isolate and genotype M. smithii in one-day-old newborns' gastric juice specimens...
August 19, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28823068/stress-affects-surface-glycoconjugates-of-the-rat-endometrium-at-the-time-of-implantation
#2
Elham Aliabadi, Zohreh Makoolati, Tahereh Talaei-Khozani, Fakhreddin Mesbah Ardekani
One of the treatments to infertility is In Vitro Fertilization (IVF). In the course of IVF, fertilization rate can be improved through stress reduction. Probably one of the causes of low outcome of IVF is changes in uterine glycoconjugates that are first site of contact between blastocyst and uterus, due to stress, e.g., stress of injection. Thus, the study of the injectional stress effects on implantation period is very important to improve the outcome of IVF. Sixteen mature female rats were divided to experimental and control groups...
August 19, 2017: Glycoconjugate Journal
https://www.readbyqxmd.com/read/28823029/experience-of-lung-transplantation-in-patients-with-lymphangioleiomyomatosis-at-a-brazilian-reference-centre
#3
Bruno Guedes Baldi, Marcos Naoyuki Samano, Silvia Vidal Campos, Martina Rodrigues de Oliveira, José Eduardo Afonso Junior, Rafael Medeiros Carraro, Ricardo Henrique Oliveira Braga Teixeira, Isabela Pasqualini Minguini, Roni Burlina, Eduardo Zinoni Silva Pato, Carlos Roberto Ribeiro Carvalho, André Nathan Costa
INTRODUCTION: Lung transplantation (LT) is the standard of care for patients with advanced lung diseases, including lymphangioleiomyomatosis (LAM). LAM accounts for only 1% of all LTs performed in the international registry. As a result, the global experience, including the use of mechanistic target of rapamycin (mTOR) inhibitors before and after LT in LAM, is still limited. METHODS: We conducted a retrospective review of all LAM patients who underwent LT at our centre between 2003 and 2016...
August 19, 2017: Lung
https://www.readbyqxmd.com/read/28822996/examining-bias-in-studies-of-statin-treatment-and-survival-in-patients-with-cancer
#4
Louise Emilsson, Xabier García-Albéniz, Roger W Logan, Ellen C Caniglia, Mette Kalager, Miguel A Hernán
Importance: Patients with cancer who use statins appear to have a substantially better survival than nonusers in observational studies. However, this inverse association between statin use and mortality may be due to selection bias and immortal-time bias. Objective: To emulate a randomized trial of statin therapy initiation that is free of selection bias and immortal-time bias. Design, Setting, and Participants: We used observational data on 17 372 patients with cancer from the Surveillance, Epidemiology, and End Results (SEER)-Medicare database (2007-2009) with complete follow-up until 2011...
August 20, 2017: JAMA Oncology
https://www.readbyqxmd.com/read/28822936/influence-of-coupled-ocean-atmosphere-phenomena-on-the-greater-horn-of-africa-droughts-and-their-implications
#5
Freddie Mpelasoka, Joseph L Awange, Ayalsew Zerihun
Drought-like humanitarian crises in the Greater Horn of Africa (GHA) are increasing despite recent progress in drought monitoring and prediction efforts. Notwithstanding these efforts, there remain challenges stemming from uncertainty in drought prediction, and the inflexibility and limited buffering capacity of the recurrent impacted systems. The complexity of the interactions of ENSO, IOD, IPO and NAO, arguably remains the main source of uncertainty in drought prediction. To develop practical drought risk parameters that potentially can guide investment strategies and risk-informed planning, this study quantifies, drought characteristics that underpin drought impacts management...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822933/occurrence-of-vibrio-species-beta-lactam-resistant-vibrio-species-and-indicator-bacteria-in-ballast-and-port-waters-of-a-tropical-harbor
#6
Charmaine Ng, Shin Giek Goh, Nazanin Saeidi, William A Gerhard, Claudia K Gunsch, Karina Yew Hoong Gin
Ballast water discharges are potential sources for the spread of invasive and pathogenic aquatic organisms. Ballast waters from six ships docked in the Port of Singapore were tested to determine if indictor organisms fell within proposed standards for ballast water discharge according to regulation D-2 of the Ballast Water Management Convention (BWMC) guidelines. Vibrio species were cultured on media supplemented with beta-lactam antibiotics to determine the presence of antibiotic resistant Vibrio species in the ballast waters of these vessels...
August 17, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822924/local-and-landscape-scale-land-cover-affects-microclimate-and-water-use-in-urban-gardens
#7
Brenda B Lin, Monika H Egerer, Heidi Liere, Shalene Jha, Peter Bichier, Stacy M Philpott
Urban gardens in Central California are highly vulnerable to the effects of climate change, experiencing both extended high heat periods as well as water restrictions because of severe drought conditions. This puts these critical community-based food production systems at risk as California is expected to experience increasing weather extremes. In agricultural systems, increased vegetation complexity, such as greater structure or biodiversity, can increase the resilience of food production systems from climate fluctuations...
August 16, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28822902/pregnancy-outcome-is-influenced-by-luteal-area-during-diestrus-before-successful-insemination-but-not-by-milk-production-level
#8
H Berger, M Lietzau, A Tichy, K Herzog
The objective was to compare luteal area (LA), luteal blood flow (LBF), and progesterone (P4) concentration before and after artificial insemination (AI) in pregnant and open cows in the diestrus preceding insemination and during the first 21 d after insemination. A number of 119 multiparous German Holstein cows were examined using B-mode and color Doppler sonography in diestrus (15-5 d before AI) and on days 7, 14, and 21 post insemination (pi). Blood samples for determination of P4 concentration were collected at each examination...
April 14, 2017: Theriogenology
https://www.readbyqxmd.com/read/28822858/comparison-of-genome-engineering-using-the-crispr-cas9-system-in-c-glabrata-wild-type-and-lig4-strains
#9
Yuke Cen, Bea Timmermans, Ben Souffriau, Johan M Thevelein, Patrick Van Dijck
Candida glabrata is reported as the second most prevalent human opportunistic fungal pathogen in North America and is threatening patients all over the world. Its incidence is rising, while it has developed resistance to the most widely used antifungal drugs, necessitating new approaches based on better insight into the biology of the organism. Despite its close phylogenetic relationship with Saccharomyces cerevisiae, generating precise genomic alterations in this species is problematic. Previously we have shown that deletion of LIG4, which encodes an enzyme involved in Non-Homologous End Joining (NHEJ), strongly enhances the probability of obtaining correctly modified transformants...
August 16, 2017: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/28822726/recurrent-out-of-hospital-cardiac-arrest
#10
Ziad Nehme, Emily Andrew, Resmi Nair, Stephen Bernard, Karen Smith
BACKGROUND: Little is known about the burden of recurrent out-of-hospital cardiac arrest (OHCA) episodes in initial survivors of OHCA. We sought to investigate the frequency of recurrent OHCA, describe time-to-event trends, and establish baseline predictors of occurrence. METHODS: Between January 2000 and June 2015, we included consecutive OHCA survivors to hospital discharge from the Victorian Ambulance Cardiac Arrest Registry. Patient identifiers were used to match index and recurrent episodes of OHCA, and death records from a government database...
August 16, 2017: Resuscitation
https://www.readbyqxmd.com/read/28822706/comparison-of-real-time-pcr-nested-pcr-and-galactomannan-antigen-detection-by-enzyme-linked-immunosorbent-assay-in-sera-for-diagnosis-of-invasive-aspergillosis
#11
S Hedhili, N Baccouchi, H Souid, B Jemli, S Gargouri
Conventional methods for diagnosis of invasive aspergillosis (IA) lack sensitivity and specificity. Serological methods still have many cases of cross-reactivity. However, molecular techniques seem to arise as a rapid approach, specific and direct that could be used in the diagnosis of IA. In this study, we analyzed 88 serum samples from patients of having IA using GM-ELISA test, nested PCR with primers for the rRNA 18S of Aspergillus genus and real time PCR specific for A. fumigatus. Among the 88 samples, 64 of them had positive GM titers and 23 had positive nested PCRs; 18 of the 23 PCR-positive samples were also GM-positive...
August 16, 2017: Journal de Mycologie Médicale
https://www.readbyqxmd.com/read/28822572/enantiodifferentiation-of-whisky-and-cognac-lactones-using-gas-chromatography-with-different-cyclodextrin-chiral-stationary-phases
#12
Hans-Georg Schmarr, Maximilian Mathes, Kristina Wall, Frank Metzner, Marius Fraefel
The chiral lactone 5-butyl-4-methyloxolan-2-one or 5-butyl-4-methyldihydro-2(3H)-furanone, often named whisky lactone, is found in oak wood, then contributing to the appreciated flavor of beverages stored in such wooden barrels. Its next higher homologue is named cognac lactone (5-pentyl-4-methyloxolan-2-one or 5-pentyl-4-methyldihydro-2(3H)-furanone), however is much less known, probably due to its minor concentration level. In order to study the direct enantioseparation of both lactones by gas chromatography on chiral stationary phases, individual enantiomers, particularly for cognac lactone were made available...
August 5, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28822558/wilms-tumor-screening-in-diffuse-capillary-malformation-with-overgrowth-and-macrocephaly-capillary-malformation-a-retrospective-study
#13
Caitlin M Peterman, Sophie Vadeboncoeur, John B Mulliken, Steven J Fishman, Marilyn G Liang
BACKGROUND: CLOVES (congenital lipomatous overgrowth, vascular malformations, epidermal nevi, and skeletal anomalies) syndrome is associated with regional bony and/or soft tissue overgrowth, capillary malformation, and an increased risk for Wilms tumor. OBJECTIVE: To evaluate the frequency of Wilms tumor in patients with 2 similar conditions: diffuse capillary malformation with overgrowth (DCMO) and macrocephaly-capillary malformation (M-CM). METHODS: Culling our Vascular Anomalies Center database, we retrospectively reviewed patients in whom DCMO and M-CM had been diagnosed and who were evaluated between 1998 and 2016 for possible development of Wilms tumor...
August 16, 2017: Journal of the American Academy of Dermatology
https://www.readbyqxmd.com/read/28822553/vitamin-d-status-in-growing-dairy-goats-and-sheep-influence-of-ultraviolet-b-radiation-on-bone-metabolism-and-calcium-homeostasis
#14
M V Nemeth, M R Wilkens, A Liesegang
The aim of this study was to investigate how controlled UVB irradiation in combination with reduced nutritional vitamin D (vitD) supply affects vitD status and Ca metabolism of growing goats and sheep. The hypothesis was that, like dairy cows, goats and sheep are able to compensate for the missing nutritional supply of vitD through endogenous production in the skin, with the consequence of a high vitD status and a balanced Ca homeostasis. Sixteen lambs and 14 goat kids aged 3 and a half months were housed in an UVB free environment and fed hay and a vitD-free concentrate over a period of 13 wk...
August 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28822289/assessing-differences-between-ostwald-ripening-and-coalescence-by-rheology-laser-diffraction-and-multiple-light-scattering
#15
J Santos, N Calero, L A Trujillo-Cayado, M C Garcia, J Muñoz
This contribution deals with the study of the influence of surfactant ratio, namely triblock copolymer (Pluronic PE9400) to polyoxyethylene glycerol fatty acid ester (Levenol C201), on the stability of emulsions formulated with a mixture of two biosolvents (N,N Dimethyl Decanamide and D-limonene), which find applications as carriers of agrochemicals. Emulsions containing Pluronic, regardless of the concentration studied, underwent Ostwald ripening while coalescence controlled the destabilization process of emulsions containing Levenol C201 as the only emulsifier...
August 14, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28822072/cellular-and-molecular-oxidative-stress-related-effects-in-uterine-myometrial-and-trophoblast-decidual-tissues-after-perigestational-alcohol-intake-up-to-early-mouse-organogenesis
#16
Tamara Anahí Coll, Gabriela Chaufan, Leticia Gabriela Pérez-Tito, Martín Ricardo Ventureira, María Del Carmen Ríos de Molina, Elisa Cebral
The placenta plays a major role in embryo-fetal defects and intrauterine growth retardation after maternal alcohol consumption. Our aims were to determine the oxidative status and cellular and molecular oxidative stress effects on uterine myometrium and trophoblast-decidual tissue following perigestational alcohol intake at early organogenesis. CF-1 female mice were administered with 10% alcohol in drinking water for 17 days prior to and up to day 10 of gestation. Control females received ethanol-free water...
August 18, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28822006/long-term-outcomes-of-adalimumab-treatment-in-254-patients-with-crohn-s-disease-a-hospital-based-cohort-study-from-korea
#17
Hyungil Seo, Byong Duk Ye, Eun Mi Song, Sun-Ho Lee, Kiju Chang, Ho-Su Lee, Sung Wook Hwang, Sang Hyoung Park, Dong-Hoon Yang, Kyung-Jo Kim, Jeong-Sik Byeon, Seung-Jae Myung, Suk-Kyun Yang
BACKGROUND AND AIMS: Large-scale studies regarding the long-term outcomes of adalimumab (ADA) treatment in Asian patients with Crohn's disease (CD) are still scarce. METHODS: We retrospectively analyzed the long-term outcomes of ADA treatment in Korean CD patients who commenced on scheduled ADA treatment at Asan Medical Center between November 2008 and July 2016. Clinical response was defined as maintaining ADA treatment without dose intensification (DI) and/or major abdominal surgery (MAS)...
August 18, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28821917/factors-determining-presence-of-passerines-breeding-within-white-stork-ciconia-ciconia-nests
#18
Adam Zbyryt, Dariusz Jakubas, Marcin Tobolka
Nests of White Stork Ciconia ciconia are commonly used by various passerines as nesting sites. In this study, we investigated factors determining presence and number of pairs of species breeding within White Stork nests in an extensive farmland in NE Poland. In 133 (57%) out of 233 White Stork nests, we found at least one breeding pair of passerine bird. These were from three species: House Sparrows Passer domesticus (68% of 133 nests with co-breeding), Tree Sparrows Passer montanus (65%), and Starlings Sturnus vulgaris (30%)...
August 18, 2017: Die Naturwissenschaften
https://www.readbyqxmd.com/read/28821808/repetition-suppression-to-objects-is-modulated-by-stimulus-specific-expectations
#19
Christian Utzerath, Elexa St John-Saaltink, Jan Buitelaar, Floris P de Lange
Repeated exposure to the same stimulus results in an attenuated brain response in cortical regions that are activated during the processing of that stimulus. This phenomenon, called repetition suppression (RS), has been shown to be modulated by expectation. Typically, this is achieved by varying the probability of stimulus repetitions (Prep) between blocks of an experiment, generating an abstract expectation that 'things will repeat'. Here, we examined whether stimulus-specific expectations also modulate RS...
August 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28821757/dated-phylogeny-and-dispersal-history-of-the-butterfly-subfamily-nymphalinae-lepidoptera-nymphalidae
#20
Chengyong Su, Qinghui Shi, Xiaoyan Sun, Junye Ma, Chunxiang Li, Jiasheng Hao, Qun Yang
The origin and dispersal history of the large butterfly subfamily Nymphalinae are not fully understood, due to internal phylogenetic and time calibration issues. We conducted phylogenetic and dating analyses using mitochondrial and nuclear genes of biogeographically diverse groups of the Nymphalinae in order to resolve some controversial relationships and the paleobiogeographic pattern of the subfamily. Our results support the sister relationship of Vanessa (Tribe Nymphalini) and the Nymphalis-group, and the grouping of the three old-world genera (Rhinopalpa, Kallimoides and Vanessula) within Tribe Victorinini...
August 18, 2017: Scientific Reports
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