Dustin Britton, Luc F Christians, Chengliang Liu, Jakub Legocki, Yingxin Xiao, Michael Meleties, Lin Yang, Michael Cammer, Sihan Jia, Zihan Zhang, Farbod Mahmoudinobar, Zuzanna Kowalski, P Douglas Renfrew, Richard Bonneau, Darrin J Pochan, Alexander J Pak, Jin Kim Montclare
Protein hydrogels represent an important and growing biomaterial for a multitude of applications, including diagnostics and drug delivery. We have previously explored the ability to engineer the thermoresponsive supramolecular assembly of coiled-coil proteins into hydrogels with varying gelation properties, where we have defined important parameters in the coiled-coil hydrogel design. Using Rosetta energy scores and Poisson-Boltzmann electrostatic energies, we iterate a computational design strategy to predict the gelation of coiled-coil proteins while simultaneously exploring five new coiled-coil protein hydrogel sequences...
December 18, 2023: Biomacromolecules