keyword
https://read.qxmd.com/read/38534926/haploidentical-hematopoietic-stem-cell-transplantation-for-patients-with-severe-aplastic-anemia-single-centre-experience
#21
Vered Stavi, Niranjan Khaire, Jeffrey H Lipton, Rajat Kumar
Severe aplastic anemia (SAA) is a life-threatening type of aplastic anemia for which allogeneic stem cell transplantation or immunosuppressive therapy are the principal treatment modalities. Only about 25-30% of patients have a matched sibling donor, and finding an unrelated donor in ethnic minorities is a challenge. The use of related haploidentical donor transplants in severe aplastic anemia is uncommon. We would like to report our experience with the first four patients who underwent haploidentical transplants for severe aplastic anemia...
February 26, 2024: Current Oncology
https://read.qxmd.com/read/38534924/modeling-5-fu-induced-chemotherapy-selection-of-a-drug-resistant-cancer-stem-cell-subpopulation
#22
JOURNAL ARTICLE
Amra Ramović Hamzagić, Danijela Cvetković, Marina Gazdić Janković, Nevena Milivojević Dimitrijević, Dalibor Nikolić, Marko Živanović, Nikolina Kastratović, Ivica Petrović, Sandra Nikolić, Milena Jovanović, Dragana Šeklić, Nenad Filipović, Biljana Ljujić
(1) Background: Cancer stem cells (CSCs) are a subpopulation of cells in a tumor that can self-regenerate and produce different types of cells with the ability to initiate tumor growth and dissemination. Chemotherapy resistance, caused by numerous mechanisms by which tumor tissue manages to overcome the effects of drugs, remains the main problem in cancer treatment. The identification of markers on the cell surface specific to CSCs is important for understanding this phenomenon. (2) Methods: The expression of markers CD24, CD44, ALDH1, and ABCG2 was analyzed on the surface of CSCs in two cancer cell lines, MDA-MB-231 and HCT-116, after treatment with 5-fluorouracil (5-FU) using flow cytometry analysis...
February 25, 2024: Current Oncology
https://read.qxmd.com/read/38534880/successful-bone-marrow-transplantation-in-a-patient-with-acute-myeloid-leukemia-developed-from-severe-congenital-neutropenia-using-modified-chemotherapy-and-conditioning-regimen-for-leukemia
#23
Risa Matsumura, Shinji Mochizuki, Yusuke Morishita, Hiroko Hayakawa, Shuhei Karakawa, Hiroshi Kawaguchi, Satoshi Okada, Nobuyuki Hyakuna, Masao Kobayashi
Severe congenital neutropenia (SCN) is characterized by chronic neutropenia with recurrent infections from early infancy and a predisposition to myelodysplastic syndrome/acute myeloid leukemia (AML). Allogeneic hematopoietic stem cell transplantation (HSCT) is the only curative treatment for patients with SCN who develop myelodysplastic syndrome/AML. We report an 8-year-old girl with SCN carrying an ELANE mutation that had been refractory to granulocyte colony-stimulating factor. The patient experienced recurrent infections and then developed AML...
February 26, 2024: Hematology Reports
https://read.qxmd.com/read/38534877/effect-of-fibrillin-2-on-differentiation-into-periodontal-ligament-stem-cell-like-cells-derived-from-human-induced-pluripotent-stem-cells
#24
JOURNAL ARTICLE
Sayuri Hamano, Daiki Yamashita, Daigaku Hasegawa, Hideki Sugii, Tomohiro Itoyama, Hidefumi Maeda
Periodontal tissue regeneration is important for preserving teeth. Periodontal ligament stem cells (PDLSCs) are useful in periodontal tissue regeneration, however tooth extraction is required to obtain these cells. Therefore, we focused on induced pluripotent stem (iPS) cells and established a method to obtain PDLSC-like cells from iPS cells. Specifically, we first differentiated iPS cells into neural crest-like cells (iNCs). Next, we obtained PDLSC-like cells (iPDLSCs) by culturing iNCs on extracellular matrix (ECM) derived from human primary periodontal ligament cells (HPDLCs)...
March 27, 2024: Stem Cells and Development
https://read.qxmd.com/read/38534757/to-repair-a-broken-heart-stem-cells-in-ischemic-heart-disease
#25
REVIEW
Theodora M Stougiannou, Konstantinos C Christodoulou, Ioannis Dimarakis, Dimitrios Mikroulis, Dimos Karangelis
Despite improvements in contemporary medical and surgical therapies, cardiovascular disease (CVD) remains a significant cause of worldwide morbidity and mortality; more specifically, ischemic heart disease (IHD) may affect individuals as young as 20 years old. Typically managed with guideline-directed medical therapy, interventional or surgical methods, the incurred cardiomyocyte loss is not always completely reversible; however, recent research into various stem cell (SC) populations has highlighted their potential for the treatment and perhaps regeneration of injured cardiac tissue, either directly through cellular replacement or indirectly through local paracrine effects...
March 8, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534739/ferroptosis-related-lncrna-to-predict-the-clinical-outcomes-and-molecular-characteristics-of-kidney-renal-papillary-cell-carcinoma
#26
JOURNAL ARTICLE
Yubo Gong, Chenchen Zhang, Hao Li, Xiaojie Yu, Yuejia Li, Zhiguo Liu, Ruyi He
Kidney renal papillary cell carcinoma (KIRP) is a highly heterogeneous type of kidney cancer, resulting in limited effective prognostic targets for KIRP patients. Long non-coding RNAs (lncRNAs) have emerged as crucial regulators in the regulation of ferroptosis and iron metabolism, making them potential targets for the treatment and prognosis of KIRP. In this study, we constructed a ferroptosis-related lncRNA risk score model (FRM) based on the TCGA-KIRP dataset, which represents a novel subtype of KIRP not previously reported...
February 29, 2024: Current Issues in Molecular Biology
https://read.qxmd.com/read/38534600/fibrin-based-hydrogels-with-reactive-amphiphilic-copolymers-for-mechanical-adjustments-allow-for-capillary-formation-in-2d-and-3d-environments
#27
JOURNAL ARTICLE
Svenja Wein, Carina Schemmer, Miriam Aischa Al Enezy-Ulbrich, Shannon Anna Jung, Stephan Rütten, Mark Kühnel, Danny Jonigk, Wilhelm Jahnen-Dechent, Andrij Pich, Sabine Neuss
This study focuses on enhancing controllable fibrin-based hydrogels for tissue engineering, addressing existing weaknesses. By integrating a novel copolymer, we improved the foundation for cell-based angiogenesis with adaptable structural features. Tissue engineering often faces challenges like waste disposal and nutrient supply beyond the 200 µm diffusion limit. Angiogenesis breaks through this limitation, allowing the construction of larger constructs. Our innovative scaffold combination significantly boosts angiogenesis, resulting in longer branches and more capillary network junctions...
March 6, 2024: Gels
https://read.qxmd.com/read/38534585/powdered-cross-linked-gelatin-methacryloyl-as-an-injectable-hydrogel-for-adipose-tissue-engineering
#28
JOURNAL ARTICLE
Tess De Maeseneer, Lana Van Damme, Merve Kübra Aktan, Annabel Braem, Paula Moldenaers, Sandra Van Vlierberghe, Ruth Cardinaels
The tissue engineering field is currently advancing towards minimally invasive procedures to reconstruct soft tissue defects. In this regard, injectable hydrogels are viewed as excellent scaffold candidates to support and promote the growth of encapsulated cells. Cross-linked gelatin methacryloyl (GelMA) gels have received substantial attention due to their extracellular matrix-mimicking properties. In particular, GelMA microgels were recently identified as interesting scaffold materials since the pores in between the microgel particles allow good cell movement and nutrient diffusion...
February 26, 2024: Gels
https://read.qxmd.com/read/38534582/synthesis-and-photopatterning-of-synthetic-thiol-norbornene-hydrogels
#29
JOURNAL ARTICLE
Umu S Jalloh, Arielle Gsell, Kirstene A Gultian, James MacAulay, Abigail Madden, Jillian Smith, Luke Siri, Sebastián L Vega
Hydrogels are a class of soft biomaterials and the material of choice for a myriad of biomedical applications due to their biocompatibility and highly tunable mechanical and biochemical properties. Specifically, light-mediated thiol-norbornene click reactions between norbornene-modified macromers and di-thiolated crosslinkers can be used to form base hydrogels amenable to spatial biochemical modifications via subsequent light reactions between pendant norbornenes in the hydrogel network and thiolated peptides...
February 23, 2024: Gels
https://read.qxmd.com/read/38534563/unlocking-the-potential-of-stem-cell-microenvironments-in-vitro
#30
REVIEW
Chiara Scodellaro, Raquel R Pina, Frederico Castelo Ferreira, Paola Sanjuan-Alberte, Tiago G Fernandes
The field of regenerative medicine has recently witnessed groundbreaking advancements that hold immense promise for treating a wide range of diseases and injuries. At the forefront of this revolutionary progress are stem cells. Stem cells typically reside in specialized environments in vivo, known as microenvironments or niches, which play critical roles in regulating stem cell behavior and determining their fate. Therefore, understanding the complex microenvironments that surround stem cells is crucial for advancing treatment options in regenerative medicine and tissue engineering applications...
March 19, 2024: Bioengineering
https://read.qxmd.com/read/38534508/contractile-and-genetic-characterization-of-cardiac-constructs-engineered-from-human-induced-pluripotent-stem-cells-modeling-of-tuberous-sclerosis-complex-and-the-effects-of-rapamycin
#31
JOURNAL ARTICLE
Veniamin Y Sidorov, Tatiana N Sidorova, Philip C Samson, Ronald S Reiserer, Clayton M Britt, M Diana Neely, Kevin C Ess, John P Wikswo
The implementation of three-dimensional tissue engineering concurrently with stem cell technology holds great promise for in vitro research in pharmacology and toxicology and modeling cardiac diseases, particularly for rare genetic and pediatric diseases for which animal models, immortal cell lines, and biopsy samples are unavailable. It also allows for a rapid assessment of phenotype-genotype relationships and tissue response to pharmacological manipulation. Mutations in the TSC1 and TSC2 genes lead to dysfunctional mTOR signaling and cause tuberous sclerosis complex (TSC), a genetic disorder that affects multiple organ systems, principally the brain, heart, skin, and kidneys...
February 28, 2024: Bioengineering
https://read.qxmd.com/read/38534431/influence-of-brainstem-s-area-a5-on-sympathetic-outflow-and-cardiorespiratory-dynamics
#32
REVIEW
Isabel Rocha, Marta González-García, Laura Carrillo-Franco, Marc Stefan Dawid-Milner, Manuel Victor López-González
Area A5 is a noradrenergic cell group in the brain stem characterised by its important role in triggering sympathetic activity, exerting a profound influence on the sympathetic outflow, which is instrumental in the modulation of cardiovascular functions, stress responses and various other physiological processes that are crucial for adaptation and survival mechanisms. Understanding the role of area A5, therefore, not only provides insights into the basic functioning of the sympathetic nervous system but also sheds light on the neuronal basis of a number of autonomic responses...
March 2, 2024: Biology
https://read.qxmd.com/read/38534417/ccl28-a-promising-biomarker-for-assessing-salivary-gland-functionality-and-maintaining-healthy-oral-environments
#33
JOURNAL ARTICLE
Yuichiro Kaibori, Saho Tamoto, Sayoko Okuda, Kazuhiko Matsuo, Takashi Nakayama, Daisuke Nagakubo
The oral cavity serves as the primary path through which substances from the outside world enter our body. Therefore, it functions as a critical component of host defense. Saliva is essential for maintaining a stable oral environment by catching harmful agents, including pathogens, allergens, and chemicals, in the air or food. CCL28, highly expressed in mucosal tissues, such as the colon and salivary glands, is a chemokine that attracts CCR10/CCR3 expressing cells. However, the role of CCL28 in salivary gland formation remains unclear...
February 27, 2024: Biology
https://read.qxmd.com/read/38534415/a-keratin-12-expression-based-analysis-of-stem-precursor-cells-and-differentiation-in-the-limbal-corneal-epithelium-using-single-cell-rna-seq-data
#34
JOURNAL ARTICLE
J Mario Wolosin
The corneal epithelium (CE) is spread between two domains, the outer vascularized limbus and the avascular cornea proper. Epithelial cells undergo constant migration from the limbus to the vision-critical central cornea. Coordinated with this migration, the cells undergo differentiation changes where a pool of unique stem/precursor cells at the limbus yields the mature cells that reach the corneal center. Differentiation is heralded by the expression of the corneal-specific Krt12. Processing data acquired by scRNA-Seq showed that the increase in Krt12 expression occurs in four distinct steps within the limbus, plus a single continuous increase in the cornea...
February 26, 2024: Biology
https://read.qxmd.com/read/38534414/the-role-of-insulin-like-growth-factor-igf-system-in-the-corneal-epithelium-homeostasis-from-limbal-epithelial-stem-cells-to-therapeutic-applications
#35
REVIEW
Małgorzata Woronkowicz, Harry Roberts, Piotr Skopiński
The corneal epithelium, comprising three layers of cells, represents the outermost portion of the eye and functions as a vital protective barrier while concurrently serving as a critical refractive structure. Maintaining its homeostasis involves a complex regenerative process facilitated by the functions of the lacrimal gland, tear film, and corneal nerves. Crucially, limbal epithelial stem cells located in the limbus (transitional zone between the cornea and the conjunctiva) are instrumental for the corneal epithelium integrity by replenishing and renewing cells...
February 25, 2024: Biology
https://read.qxmd.com/read/38534402/transgene-free-cynomolgus-monkey-ipscs-generated-under-chemically-defined-conditions
#36
JOURNAL ARTICLE
Yuliia Tereshchenko, Nesil Esiyok, Enrique Garea-Rodríguez, Daniele Repetto, Rüdiger Behr, Ignacio Rodríguez-Polo
Non-human primates (NHPs) are pivotal animal models for translating novel cell replacement therapies into clinical applications, including validating the safety and efficacy of induced pluripotent stem cell (iPSC)-derived products. Preclinical development and the testing of cell-based therapies ideally comprise xenogeneic (human stem cells into NHPs) and allogenic (NHP stem cells into NHPs) transplantation studies. For the allogeneic approach, it is necessary to generate NHP-iPSCs with generally equivalent quality to the human counterparts that will be used later on in patients...
March 21, 2024: Cells
https://read.qxmd.com/read/38534396/pros-and-cons-of-cryopreserving-allogeneic-stem-cell-products
#37
REVIEW
Caterina Giovanna Valentini, Claudio Pellegrino, Luciana Teofili
The COVID-19 pandemic has precipitously changed the practice of transplanting fresh allografts. The safety measures adopted during the pandemic prompted the near-universal graft cryopreservation. However, the influence of cryopreserving allogeneic grafts on long-term transplant outcomes has emerged only in the most recent literature. In this review, the basic principles of cell cryopreservation are revised and the effects of cryopreservation on the different graft components are carefully reexamined. Finally, a literature revision on studies comparing transplant outcomes in patients receiving cryopreserved and fresh grafts is illustrated...
March 21, 2024: Cells
https://read.qxmd.com/read/38534391/generation-of-highly-functional-hepatocyte-like-organoids-from-human-adipose-derived-mesenchymal-stem-cells-cultured-with-endothelial-cells
#38
JOURNAL ARTICLE
Shuhai Chen, Yu Saito, Yuhei Waki, Tetsuya Ikemoto, Hiroki Teraoku, Shinichiro Yamada, Yuji Morine, Mitsuo Shimada
Previously, we successfully established a highly functional, three-dimensional hepatocyte-like cell (3D-HLC) model from adipose-derived mesenchymal stem cells (ADSCs) via a three-step differentiation protocol. The aim of the present study was to investigate whether generating hepatocyte-like organoids (H-organoids) by adding endothelial cells further improved the liver-like functionality of 3D-HLCs and to assess H-organoids' immunogenicity properties. Genes representing liver maturation and function were detected by quantitative reverse transcription-PCR analysis...
March 20, 2024: Cells
https://read.qxmd.com/read/38534385/ezh2-myc-hallmark-in-oncovirus-cytomegalovirus-infections-and-cytomegalovirus-resemblance-to-oncoviruses
#39
REVIEW
Ranim El Baba, Georges Herbein
Approximately 15-20% of global cancer cases are attributed to virus infections. Oncoviruses employ various molecular strategies to enhance replication and persistence. Human cytomegalovirus (HCMV), acting as an initiator or promoter, enables immune evasion, supporting tumor growth. HCMV activates pro-oncogenic pathways within infected cells and direct cellular transformation. Thus, HCMV demonstrates characteristics reminiscent of oncoviruses. Cumulative evidence emphasizes the crucial roles of EZH2 and Myc in oncogenesis and stemness...
March 19, 2024: Cells
https://read.qxmd.com/read/38534378/deep-learning-powered-identification-of-differentiated-early-mesoderm-cells-from-pluripotent-stem-cells
#40
JOURNAL ARTICLE
Sakib Mohammad, Arpan Roy, Andreas Karatzas, Sydney L Sarver, Iraklis Anagnostopoulos, Farhan Chowdhury
Pluripotent stem cells can be differentiated into all three germ-layers including ecto-, endo-, and mesoderm in vitro. However, the early identification and rapid characterization of each germ-layer in response to chemical and physical induction of differentiation is limited. This is a long-standing issue for rapid and high-throughput screening to determine lineage specification efficiency. Here, we present deep learning (DL) methodologies for predicting and classifying early mesoderm cells differentiated from embryoid bodies (EBs) based on cellular and nuclear morphologies...
March 18, 2024: Cells
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