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https://www.readbyqxmd.com/read/29471503/the-role-of-the-reprogramming-method-and-pluripotency-state-in-gamete-differentiation-from-patient-specific-human-pluripotent-stem-cells
#1
S Mishra, E Kacin, P Stamatiadis, S Franck, M Van der Jeught, H Mertes, G Pennings, P De Sutter, K Sermon, B Heindryckx, M Geens
The derivation of gametes from patient-specific pluripotent stem cells may provide new perspectives for genetic parenthood for patients currently facing sterility. We use current data to assess the gamete differentiation potential of patient-specific pluripotent stem cells and to determine which reprogramming strategy holds the greatest promise for future clinical applications. First, we compare the two best established somatic cell reprogramming strategies: the production of induced pluripotent stem cells (iPSC) and somatic cell nuclear transfer followed by embryonic stem cell derivation (SCNT-ESC)...
February 19, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29471332/mycobacterium-tuberculosis-infected-hematopoietic-stem-and-progenitor-cells-unable-to-express-inducible-nitric-oxide-synthase-propagate-tuberculosis-in-mice
#2
Stephen T Reece, Alexis Vogelzang, Julia Tornack, Wolfgang Bauer, Ulrike Zedler, Sandra Schommer-Leitner, Georg Stingl, Fritz Melchers, Stefan H E Kaufmann
Persistence of Mycobacterium tuberculosis within human bone marrow stem cells has been identified as a potential bacterial niche during latent tuberculosis. Using a murine model of tuberculosis, we show here that bone marrow stem and progenitor cells containing M. tuberculosis propagated tuberculosis when transferred to naive mice, given that both transferred cells and recipient mice were unable to express inducible nitric oxide synthase, which mediates killing of intracellular bacteria via nitric oxide. Our findings suggest that bone marrow stem and progenitor cells containing M...
February 17, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/29471245/intraperitoneal-administration-of-mesenchymal-stem-cells-ameliorates-acute-dextran-sulfate-sodium-induced-colitis-by-suppressing-dendritic-cells
#3
Aleksandar Nikolic, Bojana Simovic Markovic, Marina Gazdic, C Randall Harrell, Crissy Fellabaum, Nemanja Jovicic, Valentin Djonov, Nebojsa Arsenijevic, Miodrag L Lukic, Miodrag Stojkovic, Vladislav Volarevic
Dendritic cells (DCs) have important pathogenic role in the induction and progression of ulcerative colitis (UC), but their role in mesenchymal stem cells (MSCs)-mediated suppression of colon injury and inflammation is not revealed. By using dextran sodium sulfate (DSS)-induced colitis, a well-established murine model of UC, we examined effects of MSCs on phenotype and function of colon infiltrating DCs. Clinical, histological, immunophenotypic analysis and passive transfer of MSCs-primed DCs were used to evaluate capacity of MSC to suppress inflammatory phenotype of DCs in vivo...
February 19, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29471171/triggering-the-activation-of-activin-a-type-ii-receptor-in-human-adipose-stem-cells-towards-tenogenic-commitment-using-mechanomagnetic-stimulation
#4
A I Gonçalves, M Rotherham, H Markides, M T Rodrigues, R L Reis, M E Gomes, A J El Haj
Stem cell therapies hold potential to stimulate tendon regeneration and homeostasis, which is maintained in response to the native mechanical environment. Activins are members of the mechano-responsive TGF-β superfamily that participates in the regulation of several downstream biological processes. Mechanosensitive membrane receptors such as activin can be activated in different types of stem cells via magnetic nanoparticles (MNPs) through remote magnetic actuation resulting in cell differentiation. In this work, we target the Activin receptor type IIA (ActRIIA) in human adipose stem cells (hASCs), using anti-ActRIIA functionalized MNPs, externally activated through a oscillating magnetic bioreactor...
February 19, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29471169/pegylated-dendritic-polyglycerol-conjugate-targeting-ncam-expressing-neuroblastoma-limitations-and-challenges
#5
Laura Isabel Vossen, Ela Markovsky, Anat Eldar-Boock, Harald Rune Tschiche, Stefanie Wedepohl, Evgeny Pisarevsky, Ronit Satchi-Fainaro, Marcelo Calderón
Neural cell adhesion molecule (NCAM) is found to be a stem-cell marker in several tumor types and its overexpression is known to correlate with increased metastatic capacity. To combine extravasation- and ligand-dependent targeting to NCAM overexpressing-cells in the tumor microenvironment, we developed a PEGylated NCAM-targeted dendritic polyglycerol (PG) conjugate. Here, we describe the synthesis, physico-chemical characterization and biological evaluation of a PG conjugate bearing the mitotic inhibitor paclitaxel (PTX) and an NCAM-targeting peptide (NTP)...
February 19, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29471105/icariin-doped-bioactive-glasses-seeded-with-rat-adipose-derived-stem-cells-to-promote-bone-repair-via-enhanced-osteogenic-and-angiogenic-activities
#6
Xingzhi Jing, Weifeng Yin, Hongtao Tian, Mengcun Chen, Xudong Yao, Wentao Zhu, Fengjing Guo, Yaping Ye
AIMS: Cell communication between mesenchymal stem cells and blood vessel cells are crucial for bone repair. We have previously shown that the phyto-molecule icariin significantly promoted osteogenic differentiation of rat adipose-derived stem cells (ASCs). In the present study, we aimed to investigate the relationship between icariin induced osteogenic differentiation of ASCs and angiogenesis of rat endothelial progenitor cells (EPCs). Besides, we used icariin doped 45S5 Bioglass seeded with ASCs to promote bone healing in rat calvarial bone defect models...
February 19, 2018: Life Sciences
https://www.readbyqxmd.com/read/29471042/aspirin-induced-attenuation-of-adipogenic-differentiation-of-bone-marrow-mesenchymal-stem-cells-is-accompanied-by-the-disturbed-epigenetic-modification
#7
Yuanbo Zhan, Zhiwei He, Xinpeng Liu, Nan Miao, Feng Lin, Sen Mu, Haibin Mu, Mengtong Yuan, Xiaofang Cao, Han Jin, Zhongshuang Liu, Ying Li, Bin Zhang
Aspirin has positive effects on bone marrow mesenchymal stem cells (BMSCs) osteogenic differentiation. However, researchers did not give much thought to its effect on BMSCs adipogenic differentiation. Here, we analyzed the effect of aspirin on the BMSCs adipogenic differentiation. To detect whether the effect of aspirin on the adipogenic differentiation of BMSCs is associated with the disturbed epigenetic modification, the expression of histone deacetylases (HDACs), activity of HDACs and HAT, global histone H3 acetylation and H3k9 acetylation alterations were investigated...
February 19, 2018: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/29471034/microbiome-host-interactions-in-hematopoietic-stem-cell-transplant-recipients
#8
Tessa Andermann, Jonathan Peled, Christine Ho, Pavan Reddy, Marcie Riches, Rainer Storb, Takanori Teshima, Marcel van den Brink, Amin Alousi, Sophia Balderman, Patrizia Chiusolo, William Clark, Ernst Holler, Alan Howard, Leslie Kean, Andrew Koh, Philip McCarthy, John McCarty, Mohamad Mohty, Ryotaro Nakamura, Katy Rezvani, Brahm Segal, Bronwen Shaw, Elizabeth Shpall, Anthony Sung, Daniela Weber, Jennifer Whangbo, John Wingard, William Wood, Miguel-Angel Perales, Robert Jenq, Ami Bhatt
No abstract text is available yet for this article.
February 19, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29471033/inhibition-of-lymphocyte-proliferation-an-ability-shared-by-murine-mesenchymal-stem-cells-dermal-fibroblasts-and-chondrocytes
#9
Shang Zhang, Haitao Wu, Changlin Liu
It has been demonstrated that mesenchymal stem cells (MSCs) have potent immunosuppressive capacities. But it is controversial whether differentiated mature stromal cells (SCs) share the immunosuppressive capacities. A previous study examined the ability of SCs from different human tissue sites to inhibit the proliferation of lymphocytes. The results are all positive but the mechanism isn't clear, and few mouse data have been published on this topic. Using an efficient mixed cell culture assay, our in vitro data show that the anti-proliferative ability of murine MSCs on lymphocytes is shared by mature murine SCs, i...
February 19, 2018: Transplant Immunology
https://www.readbyqxmd.com/read/29471019/hsp90-co-chaperone-p23-contributes-to-dopaminergic-mitochondrial-stress-via-stabilization-of-phd2-implications-for-parkinson-s-disease
#10
Anand Rane, Subramanian Rajagopalan, Manuj Ahuja, Bobby Thomas, Shankar J Chinta, Julie K Andersen
The heat shock factor 90 (hsp90) complex has long been associated with neuropathological phenotypes linked to Parkinson's disease (PD) and its inhibition is neuroprotective in disease models. Hsp90 is conventionally believed to act by suppressing induction of hsp70. Here, we report a novel hsp70-independent mechanism by which Hsp90 may also contribute to PD-associated neuropathology. We previously reported that inhibition of the enzyme prolyl hydroxylase domain 2 (PHD2) in conjunction with increases in hypoxia-inducible factor 1 alpha (HIF1α) results in protection of vulnerable dopaminergic substantia nigra pars compacta (DAergic SNpc) neurons in in vitro and in vivo models of PD...
February 19, 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29471003/trkb-receptor-antagonism-inhibits-stab-injury-induced-proliferative-response-in-adult-zebrafish-danio-rerio-brain
#11
Surendra Kumar Anand, Amal Chandra Mondal
The Tropomyosin related kinase (TrkB) receptor, is known to promote neuronal maturation, differentiation, maintenance and survival through its cognate ligands Brain derived neurotrophic factor (BDNF) and neurotrophin 4 (NT4). BDNF, NT4 and TrkB are highly conserved proteins among vertebrates. Although the role of TrkB during brain development is well established, its role in adult neurogenesis and brain regeneration awaits thorough investigation. In this study, we used the zebrafish stab wound injury model to determine whether the injury induced regeneration response in the telencephalon region is governed by TrkB or not...
February 19, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29470992/control-of-cell-fraction-and-population-recovery-during-tissue-regeneration-in-stem-cell-lineages
#12
Marissa Renardy, Alexandra Jilkine, Leili Shahriyari, Ching-Shan Chou
Multicellular tissues are continually turning over, and homeostasis is maintained through regulated proliferation and differentiation of stem cells and progenitors. Following tissue injury, a dramatic increase in cell proliferation is commonly observed, resulting in rapid restoration of tissue size. This regulation is thought to occur via multiple feedback loops acting on cell self-renewal or differentiation. Models of ordinary differential equations have been widely used to study the cell lineage system. Prior modeling studies have suggested that loss of homeostasis and initiation of tumorigenesis can be contributed to the loss of control of these processes, and the rate of symmetric versus asymmetric division of the stem cells may also be altered...
February 19, 2018: Journal of Theoretical Biology
https://www.readbyqxmd.com/read/29470984/ribosome-biogenesis-protein-urb2-regulates-hematopoietic-stem-cells-development-via-p53-pathway-in-zebrafish
#13
Pengcheng Cai, Xiaoyu Mao, Jieqiong Zhao, Lingfei Luo
Ribosome biogenesis is a significant process in cells. Dysfunction in this process will result in the defects of protein synthesis and consequently cause the development of specific diseases called ribosomopathies. Mutations in ribosome biogenesis protein Rps19, Rpl5, or Rpl11 can lead to hematopoietic defects in human, thus triggering the disease Diamond Blackfan anemia. However, the regulatory mechanisms of ribosome biogenesis in hematopoiesis remain incompletely understood. In this study, we describe a zebrafish mutant cq42, which carries a nonsense mutation in the gene that encodes ribosome biogenesis 2 homolog (Urb2)...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29470980/graphene-coating-on-the-surface-of-cocrmo-alloy-enhances-the-adhesion-and-proliferation-of-bone-marrow-mesenchymal-stem-cells
#14
Qi Zhang, Kewen Li, Jinhong Yan, Zhuo Wang, Qi Wu, Long Bi, Min Yang, Yisheng Han
The objective was to investigate whether a graphene coating could improve the surface bioactivity of a cobalt-chromium-molybdenum-based alloy (CoCrMo). Graphene was produced by chemical vapor deposition and transferred to the surface of the CoCrMo alloy using an improved wet transfer approach. The morphology of the samples was observed, and the adhesion force and stabilization of graphene coating were analyzed by a nanoscratch test and ultrasonication test. In an in vitro studies, the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) cultured on the samples were quantified via an Alamar Blue assay and cell counting kit-8 (CCK-8) assay...
February 19, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29470831/expression-and-function-of-immune-ligand-receptor-pairs-in-nk-cells-and-cancer-stem-cells-therapeutic-implications
#15
REVIEW
Ioannis A Voutsadakis
BACKGROUND: The interplay between the immune system and cancer cells has come to the forefront of cancer therapeutics, with novel immune blockade inhibitors being approved for the treatment of an increasing list of cancers. However, the majority of cancer patients still display or develop resistance to these promising drugs. It is possible that cancer stem cells (CSCs) are contributing to this therapeutic resistance. Although CSCs usually represent a small percentage of the total number of cancer cells, they are endowed with the ability of self-renewal and to produce differentiated progeny...
February 22, 2018: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/29470822/the-concept-of-the-ccn-protein-family-revisited-a-centralized-coordination-network
#16
REVIEW
Bernard Perbal
The wide array of biological properties attributed to the CCN family of proteins (Perbal in Lancet 363(9402):62-64, 2004) led me to reconsider the possible relationship and roles that these proteins may play as a team, instead of acting on their own as individual regulators in various signaling pathways. The dynamic model which I present in this review stems from the contribution of the biological properties that we established for CCN3, one of the three founding members of the CCN family, which was identified by our group as the first CCN protein showing growth inhibitory properties (1992), expressed mainly in quiescent cells (1996), and showing anti-tumor activities in several cellular models both ex vivo and in vivo...
February 22, 2018: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/29470694/new-prospects-of-mesenchymal-stem-cells-for-ameliorating-temporal-lobe-epilepsy
#17
Neveen A Salem, Marwa El-Shamarka, Yasser Khadrawy, Shaimaa El-Shebiney
Temporal lobe epilepsy (TLE) is present in 30% of epileptic patients and does not respond to conventional treatments. Bone marrow derived mesenchymal stem cells (BMSCs) induce endogenous neural stem cells, inhibit neurodegeneration, and promote brain self-repair mechanisms. The present study addresses the feasibility of BMSCs transplantation against pilocarpine-induced TLE experimentally. BMSCs were injected either intravenously (IV) or in hippocampus bilaterally (IC). Increased cell count of BMSCs was achieved via IC route...
February 22, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29470523/a-late-but-fitter-revertant-cell-explains-the-high-frequency-of-revertant-mosaicism-in-epidermolysis-bullosa
#18
Peter C van den Akker, Anna M G Pasmooij, Hans Joenje, Robert M W Hofstra, Gerard J Te Meerman, Marcel F Jonkman
Revertant mosaicism, or "natural gene therapy", is the phenomenon in which germline mutations are corrected by somatic events. In recent years, revertant mosaicism has been identified in all major types of epidermolysis bullosa, the group of heritable blistering disorders caused by mutations in the genes encoding epidermal adhesion proteins. Moreover, revertant mosaicism appears to be present in all patients with a specific subtype of recessive epidermolysis bullosa. We therefore hypothesized that revertant mosaicism should be expected at least in all patients with recessive forms of epidermolysis bullosa...
2018: PloS One
https://www.readbyqxmd.com/read/29470464/generation-of-human-brain-region-specific-organoids-using-a-miniaturized-spinning-bioreactor
#19
Xuyu Qian, Fadi Jacob, Mingxi Max Song, Ha Nam Nguyen, Hongjun Song, Guo-Li Ming
Human brain organoids, 3D self-assembled neural tissues derived from pluripotent stem cells, are important tools for studying human brain development and related disorders. Suspension cultures maintained by spinning bioreactors allow for the growth of large organoids despite the lack of vasculature, but commercially available spinning bioreactors are bulky in size and have low throughput. Here, we describe the procedures for building the miniaturized multiwell spinning bioreactor SpinΩ from 3D-printed parts and commercially available hardware...
March 2018: Nature Protocols
https://www.readbyqxmd.com/read/29470431/articular-cartilage-aging-potential-regenerative-capacities-of-cell-manipulation-and-stem-cell-therapy
#20
REVIEW
Magdalena Krajewska-Włodarczyk, Agnieszka Owczarczyk-Saczonek, Waldemar Placek, Adam Osowski, Joanna Wojtkiewicz
Changes in articular cartilage during the aging process are a stage of natural changes in the human body. Old age is the major risk factor for osteoarthritis but the disease does not have to be an inevitable consequence of aging. Chondrocytes are particularly prone to developing age-related changes. Changes in articular cartilage that take place in the course of aging include the acquisition of the senescence-associated secretory phenotype by chondrocytes, a decrease in the sensitivity of chondrocytes to growth factors, a destructive effect of chronic production of reactive oxygen species and the accumulation of the glycation end products...
February 22, 2018: International Journal of Molecular Sciences
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