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Lean microbiology

Lena Schuster, Corinna Franke, Patrick Silcock, Jonathan Beauchamp, Phil J Bremer
The impact of different ratios of lean meat, adipose tissue (fat) and bone on the volatile organic compound (VOC) profile of vacuum-packed (VP) lamb stored at 2 °C for up to 15 days was investigated using two sampling approaches. VOC development in individual samples was followed over time using either a traditional sampling regime where replicate samples were sampled (single-use) at a given time or a novel approach where replicate samples were resampled (reuse) over time. VOCs present in the headspace of the packaged samples were detected using proton-transfer-reaction mass spectrometry (PTR-MS) with complementary solid phase micro-extraction gas chromatography-mass spectrometry (SPME-GC-MS) analysis on a subset of samples...
May 24, 2018: Meat Science
Aida Lahmar, David Morcuende, María-Jesús Andrade, Leila Chekir-Ghedira, Mario Estévez
The aim of this study was to assess the effectiveness of sprayable essential oils for counteracting the pro-oxidant environment created during retail display of lamb cutlets packed under high-oxygen atmosphere. North-African essential oils from Pituranthos chloranthus (PC) and Teucrium ramosissimum (TR) were applied by spraying on the surface of whole-lamb cutlets. The bio-preservative treatment led to a significant increase of their shelf life in terms of protection against lipid and protein oxidative deterioration, discoloration of lean and bone and microbiological stability during 12days of chilled storage...
May 2018: Meat Science
Y Sánchez-Roque, Y D C Pérez-Luna, E Pérez-Luna, R Berrones Hernández, S Saldaña-Trinidad
AIM: This study was conducted to evaluate the physiological and biochemical effect in chickens of the Ross breed of a food enriched with agroindustrial waste. MATERIALS AND METHODS: The food is one of the main components of the total cost for the production of chickens. Rations should be formulated to provide the correct balance of energy, protein, amino acids, minerals, vitamins and essential fatty acids, to allow optimal growth and performance. This study was intended to evaluate a natural feed for chicken, made from corn, yucca meal, eggshells, orange peel, soybean meal, salt and garlic, enriched with agroindustrial waste (molasses, milk whey and ferment of coffee)...
April 2017: Veterinary World
Teresa Requena, Marta Miguel, Marta Garcés-Rimón, M Carmen Martínez-Cuesta, Rosina López-Fandiño, Carmen Peláez
There is limited information that relates the intake of food-derived bioactive peptides and the gut microbiota. We have previously described a pepsin hydrolysate of egg white (EWH) that ameliorates fat accumulation and dyslipidemia, while reducing oxidative stress and inflammation markers in obese Zucker rats. The aim of this study was to associate the beneficial effects of EWH with gut microbiota changes in these animals. Obese Zucker rats received daily 750 mg kg(-1) EWH in drinking water for 12 weeks and faeces were analysed for microbial composition and metabolic compounds in comparison with Zucker lean rats and obese controls...
January 16, 2017: Food & Function
Leslie Altic, Helene McNulty, Leane Hoey, Liadhan McAnena, Kristina Pentieva
Functional foods enriched with folate may be beneficial as a means of optimizing folate status in consumers. We recently developed novel eggs enriched with folate through folic acid supplementation of the hen's feed, but their potential to influence consumer folate status is unknown because the natural folate forms incorporated into the eggs may not necessarily be retained during storage and cooking. This study aimed to determine the stability of natural folates in folate-enriched eggs under typical conditions of storage and cooking...
November 30, 2016: Nutrients
Jonathan Cloke, Sharon Matheny, Michelle Swimley, Robert Tebbs, Angelia Burrell, Jonathan Flannery, Benjamin Bastin, Patrick Bird, M Joseph Benzinger, Erin Crowley, James Agin, David Goins, Yvonne Salfinger, Michael Brodsky, Maria Cristina Fernandez
The Applied Biosystems™ RapidFinder™ STEC Detection Workflow (Thermo Fisher Scientific) is a complete protocol for the rapid qualitative detection of Escherichia coli (E. coli) O157:H7 and the "Big 6" non-O157 Shiga-like toxin-producing E. coli (STEC) serotypes (defined as serogroups: O26, O45, O103, O111, O121, and O145). The RapidFinder STEC Detection Workflow makes use of either the automated preparation of PCR-ready DNA using the Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit in conjunction with the Applied Biosystems MagMAX™ Express 96-well magnetic particle processor or the Applied Biosystems PrepSEQ Rapid Spin kit for manual preparation of PCR-ready DNA...
November 1, 2016: Journal of AOAC International
Patrick Bird, Jonathan Flannery, Erin Crowley, James R Agin, David Goins, Lisa Monteroso
The 3M™ Molecular Detection Assay (MDA) 2 - Salmonella uses real-time isothermal technology for the rapid and accurate detection of Salmonella spp. from enriched select food, feed, and food-process environmental samples. The 3M MDA 2 - Salmonella was evaluated in a multilaboratory collaborative study using an unpaired study design. The 3M MDA 2 - Salmonella was compared to the U.S. Food and Drug Administration Bacteriological Analytical Manual Chapter 5 reference method for the detection of Salmonella in creamy peanut butter, and to the U...
July 2016: Journal of AOAC International
Ryan Viator, Susan Alles, Quynh-Nhi Le, Edan Hosking, Evan Meister, Lisa Pinkava, Eric Tovar, Mark Mozola, Jennifer Rice
A performance validation of the ANSR(®) for E. coli O157:H7 method was conducted in selected food matrixes. This assay uses selective nicking enzyme amplification technology to amplify target genes. Samples are enriched for 12-24 h and then lysed. The assay is completed within 40 min using real-time detection in a combination incubator/fluorescence detector and software. When 44 distinct strains of Escherichia coli O157:H7 and 6 strains of E. coli O157:NM were tested for inclusivity, all 50 strains produced positive results...
May 2016: Journal of AOAC International
S Ghosh, S Mohanty, A Akcil, L B Sukla, A P Das
In view of unremitting diminution of mineral resources, rising energy economics along with increasing global consumption of Manganese (Mn), development of environment friendly technologies for tapping alternate sources of Mn has gained importance lately. Mn recovery from mining residues using conventional approaches is extremely expensive due to high capital and energy costs involved. However lean grade ores present in millions of tons awaits the development of competent and cost effective extractive process...
July 2016: Chemosphere
(no author information available yet)
A performance validation of the ANSR(®) for E. coli O157:H7 method was conducted in selected food matrixes. This assay uses selective nicking enzyme amplification technology to amplify target genes. Samples are enriched for 12-24 h and then lysed. The assay is completed within 40 min using real-time detection in a combination incubator/fluorescence detector and software. When 44 distinct strains of Escherichia coli O157:H7 and 6 strains of E. coli O157:NM were tested for inclusivity, all 50 strains produced positive results...
March 31, 2016: Journal of AOAC International
Jennifer L Ather, Michael Chung, Laura R Hoyt, Matthew J Randall, Anna Georgsdottir, Nirav A Daphtary, Minara I Aliyeva, Benjamin T Suratt, Jason H T Bates, Charles G Irvin, Sheila R Russell, Patrick M Forgione, Anne E Dixon, Matthew E Poynter
Obese asthma presents with inherent hyperresponsiveness to methacholine or augmented allergen-driven allergic asthma, with an even greater magnitude of methacholine hyperresponsiveness. These physiologic parameters and accompanying obese asthma symptoms can be reduced by successful weight loss, yet the underlying mechanisms remain incompletely understood. We implemented mouse models of diet-induced obesity, dietary and surgical weight loss, and environmental allergen exposure to examine the mechanisms and mediators of inherent and allergic obese asthma...
August 2016: American Journal of Respiratory Cell and Molecular Biology
Gabriela Angelina Reartes, Romina Daniela Di Paola Naranjo, Aldo Renato Eynard, Sonia Edith Muñoz
Content of carcinogenic molecules like, 2-Amino, 1-methyl, 6-phenylimidazo[4,5-b] pyridine in meals is one of the main mutagenic substances formed during meat cooking, and it can be used as a dietary exposure marker. Our objective was to estimate the amount of PhIP consumed from habitual Argentinean diet, rich in red meats, comparing different cooking procedures and meat type. Samples (n = 240) of lean and fatty beef, chicken, pork, and fish were cooked using different methods: griddle, grill, sauté pan, and oven...
June 2016: Food and Chemical Toxicology
Benjamin A White, Jason M Baron, Anand S Dighe, Carlos A Camargo, David F M Brown
BACKGROUND: Increasing the value of health care delivery is a national priority, and providers face growing pressure to reduce cost while improving quality. Ample opportunity exists to increase efficiency and quality simultaneously through the application of systems engineering science. OBJECTIVE: We examined the hypothesis that Lean-based reorganization of laboratory process flow would improve laboratory turnaround times (TAT) and reduce waste in the system. METHODS: This study was a prospective, before-after analysis of laboratory process improvement in a teaching hospital emergency department (ED)...
November 2015: American Journal of Emergency Medicine
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Robert Jechorek
The 3M™ Petrifilm™ Rapid Yeast and Mold (RYM) Count Plate is a simple, ready-to-use chromogenic culture method for the rapid detection and enumeration of yeast and mold in food products. The 3M Petrifilm RYM Count Plate method was compared to the U. S. Food and Drug Administration Bacteriological Analytical Manual (FDA BAM) Chapter 18, Yeasts, Molds and Mycotoxins and the ISO 21527:2008 Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Enumeration for Yeast and Molds - Part 1: Colony Count Technique in Products with Water Activity Greater Than 0...
May 2015: Journal of AOAC International
Heena Sharma, B D Sharma, S Talukder, Giriprasad Ramasamy
Use of extenders in meat products is not only health promoting but can also increase the economic worth of the products. Extension of the meat product is generally associated with poor binding and texture. Thus, the present study was envisaged to solve this problem by the incorporation of gum tragacanth (GT) as bind enhancing agent, used at three different levels viz., 0.1, 0.15 and 0.2 % in a pre standardized formulation of extended restructured mutton chops (ERMC), by replacing the lean meat. The products were subjected to analysis for physico-chemical, sensory and textural properties...
March 2015: Journal of Food Science and Technology
Hilzeth de Luna Freire Pessôa, Maria Lúcia da Conceição, Andrea Maria Rolim da Paz, Bagnólia Araújo da Silva, Maria José de Carvalho Costa
Pomacea lineata is a fresh water shellfish that is utilized as food, medicinal portions, or licking remedies. The objective of this study was to evaluate the nutritional and microbiological aspects of P. lineata and spawning. The samples were collected in the Jaguaribe River (Paraíba, Brazil) during the rainy and dry seasons. The biochemical composition, mineral composition, total mesophilic bacteria (TMB), total coliforms (TC), fecal coliforms (FC), Salmonella sp., Staphylococcus aureus, and Vibrio presence were determined...
July 2015: Journal of Medicinal Food
Heena Sharma, Brahma Deo Sharma, S K Mendiratta, Suman Talukder, Giriprasad Ramasamy
Consumers have become very conscious about their nutrition and well being due to changes in their socio-economic lifestyle and rapid urbanization. Therefore, development of technology for production of low cost and functional meat products is urgently required. One such approach is innovative restructuring technology in which binding of meat pieces still remains the main challenge and extension of product is generally associated with poor binding and texture. Thus, the present study was envisaged as an attempt to solve this problem by the incorporation of flaxseed flour (FF) as bind enhancing agent...
February 2014: Asian-Australasian Journal of Animal Sciences
Aurelio Briones, Erik Coats, Cynthia Brinkman
Conventional anaerobic digesters (ADs) treating dairy manure are fed with raw or fermented manure rich in volatile fatty acids (VFAs). In contrast, pre-fermented AD (PF-AD) is fed with the more recalcitrant, fiber-rich fraction of manure that has been pre-fermented and depleted of VFAs. Thus, the substrate of PF-AD may be likened to a lean diet rich in fibers while the pre-fermentation stage fermenter is fed a relatively rich diet containing labile organic substances. Previous results have shown that conventional and pre-fermented ADs fed with raw or pre-fermented manure, respectively, produced comparable methane yields...
2014: PloS One
P Shawn Mitchell, Jayawant N Mandrekar, Joseph D C Yao
Clinical laboratories are constantly facing challenges to do more with less, enhance quality, improve test turnaround time, and reduce operational expenses. Experience with adopting and applying lean concepts and tools used extensively in the manufacturing industry is described for a high-volume clinical molecular microbiology laboratory, illustrating how operational success and benefits can be achieved.
July 2014: Journal of Clinical Microbiology
Linoj Samuel, Susan Novak-Weekley
This commentary will introduce lean concepts into the clinical microbiology laboratory. The practice of lean in the clinical microbiology laboratory can remove waste, increase efficiency, and reduce costs. Lean, Six Sigma, and other such management initiatives are useful tools and can provide dividends but must be accompanied by organizational leadership commitment to sustaining the lean culture in the laboratory setting and providing resources and time to work through the process.
June 2014: Journal of Clinical Microbiology
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