Read by QxMD icon Read

Carbapenemase screening

Laurent Dortet, Sandrine Bernabeu, Camille Gonzalez, Thierry Naas
The objective of this study was to assess the performance of the Carbapenemase Detection Set™ (CDS; Mast Diagnostics) in association with i) the EUCAST meropenem screening cut-off and ii) the faropenem-temocillin algorithm (FTa) for the screening of carbapenemase-producing Enterobacteriaceae (CPE). A total of 200 well-characterized enterobacterial isolates with reduced susceptibility to at least one carbapenem including 63 non-CPEs and 137 CPEs belonging to different Ambler classes were initially screened for CPEs using i) the EUCAST meropenem cut-off (diameter <25 mm) and ii) the FTa...
February 17, 2018: Diagnostic Microbiology and Infectious Disease
Omnia A Shaker, Howayda E Gomaa, Shereen A ElMasry, Rania M Abdel Halim, Amany H Abdelrahman, Jackline S Kamal
AIM: To evaluate the diagnostic performance of MDI and temocillin disk (30 μg) for detection of carbapenem-resistant Enterobacteriaceae in comparison to real-time PCR. MATERIAL AND METHODS: Fifty specimens submitted to the Microbiology Laboratory of Ain Shams University Hospitals and showed resistance to carbapenem drugs through routine culture and susceptibility testing, were assessed by both temocillin disk (30 μg) and MDI set to detect carbapenem-resistant Enterobacteriaceae ...
February 15, 2018: Open Access Macedonian Journal of Medical Sciences
Caroline M Coope, Neville Q Verlander, Annegret Schneider, Susan Hopkins, William Welfare, Alan P Johnson, Bharat Patel, Isabel Oliver
BACKGROUND: Following hospital outbreaks of carbapenemase-producing Enterobacteriaceae (CPE), Public Health England published a toolkit in December 2013 to promote the early detection, management and control of CPE colonisation and infection in acute hospital settings. AIM: This evaluation aimed to examine awareness, uptake, implementation and usefulness of the CPE toolkit and identify potential barriers and facilitators to its adoption in order to inform future guidance...
March 9, 2018: Journal of Hospital Infection
Elisabetta Nucleo, Mariasofia Caltagirone, Vittoria Mattioni Marchetti, Roberto D'Angelo, Elena Fogato, Massimo Confalonieri, Camilla Reboli, Albert March, Ferisa Sleghel, Gertrud Soelva, Elisabetta Pagani, Richard Aschbacher, Roberta Migliavacca, Laura Pagani
Background: Rationale and aims of the study were to compare colonization frequencies with MDR bacteria isolated from LTCF residents in three different Northern Italian regions, to investigate risk factors for colonization and the genotypic characteristics of isolates. The screening included Enterobacteriaceae expressing extended-spectrum β-lactamases (ESβLs) and high-level AmpC cephalosporinases, carbapenemase-producing Enterobacteriaceae , Pseudomonas aeruginosa or Acinetobacter baumannii , methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE)...
2018: Antimicrobial Resistance and Infection Control
Michael A Pfaller, Michael D Huband, Rodrigo E Mendes, Robert K Flamm, Mariana Castanheira
We evaluated meropenem-vaborbactam activity against 11,559 Enterobacteriaceae isolates, including 330 carbapenem-resistant phenotypes (CRE) and carbapenemase genotypes collected worldwide during 2015. Meropenem-vaborbactam (inhibitor at 8 mg/L) and comparators were susceptibility tested using reference broth microdilution methods. CRE isolates were screened for the presence of genes encoding carbapenemases and 292 (88.5%) of the CRE isolates carried these resistance genes. A total of 209 (63.3% of the CRE; 1...
March 3, 2018: International Journal of Antimicrobial Agents
Ryota Gomi, Tomonari Matsuda, Masaki Yamamoto, Pei-Hsin Chou, Michio Tanaka, Satoshi Ichiyama, Minoru Yoneda, Yasufumi Matsumura
Wastewater is considered a major source of antibiotic-resistant bacteria released into the environment. Here, we characterized carbapenemase-producing Enterobacteriaceae (CPE) in wastewater by whole-genome analysis. Wastewater samples (n = 40) were collected from municipal wastewater treatment plants and hospital wastewater in Japan and Taiwan. Samples were screened for CPE using selective media, and the obtained isolates were sequenced using an Illumina MiSeq. The isolates (n = 45) included the following microorganisms: Klebsiella quasipneumoniae (n = 12), Escherichia coli (n = 10), Enterobacter cloacae complex (n = 10), Klebsiella pneumoniae (n = 8), Klebsiella variicola (n = 2), Raoultella ornithinolytica (n = 1), Citrobacter freundii (n = 1), and Citrobacter amalonaticus (n = 1)...
February 26, 2018: Antimicrobial Agents and Chemotherapy
Francis Onwugamba, J Ross Fitzgerald, Kateryn Rochon, Luca Guardabassi, Abraham Alabi, Stefan Kühne, Martin P Grobusch, Frieder Schaumburg
BACKGROUND: 'Filth flies' feed and develop in excrement and decaying matter and can transmit enteric pathogens to humans and animals, leading to colonization and infection. Considering these characteristics, 'filth flies' are potential vectors for the spread of antimicrobial resistance (AMR). This review defines the role of flies in the spread of AMR and identifies knowledge gaps. METHODS: The literature search (original articles, reviews indexed for PubMed) was restricted to the English language...
February 23, 2018: Travel Medicine and Infectious Disease
Joo-Hee Hwang, Jeong Su Park, Eunyoung Lee, Ji Yun Bae, Kyoung-Ho Song, Pyoeng Gyun Choe, Wan Beom Park, Ji Hwan Bang, Eu Suk Kim, Sang Won Park, Nam Joong Kim, Myoung-Don Oh, Hong Bin Kim
A 10-month active surveillance study was conducted to assess carriage of carbapenemase-producing Enterobacteriaceae (CPE), vancomycin-resistant enterococci (VRE) and toxigenic Clostridium difficile colonization among patients transferred to hospital from long-term care facilities (LTCFs). Four (1.4%) carbapenem-resistant Enterobacteriaceae (none CPE), 59 (21%) VRE carriers, and 20 (7.1%) persons colonized with toxigenic C. difficile were identified from 282 rectal specimens. There was no outbreak of VRE infection during the study period...
February 21, 2018: Journal of Hospital Infection
Shuxian Wen, Donghua Feng, Zerong Lu, Junyan Liu, Brian M Peters, Hailing Tang, Danhong Su, Yong-Ping Lin, Ling Yang, Zhenbo Xu, Mark E Shirtliff, Dingqiang Chen
BACKGROUND: Carbapenem-resistant Gram-negative bacilli (GNB) have become an important cause of nosocomial infections of hospitalized patients. METHODS: To investigate the microbial infection patterns and molecular epidemiology characteristics of the carbapenem-resistant GNB isolates from a long-term hospitalized patient, antimicrobial susceptibility testing, phenotypic screening test for carbapenemase production, PCR screening and DNA sequencing of carbapenemase genes, repetitive extragenic palindromic sequence-based PCR (REP-PCR), multilocus sequencing typing (MLST) and genetic environment analysis were performed...
February 13, 2018: Microbial Pathogenesis
C Legeay, V Thépot-Seegers, H Pailhoriès, D Hilliquin, J-R Zahar
BACKGROUND: Carbapenemase producing Enterobacteriaceae (CPE) area a major issue. Cohorting can help controlling spread of CPEs in hospitals, but is expensive and hard to implement. AIM: To identify ward variables associated with CPE in-hospital transmission in a hospital where cohorting was never implemented. METHODS: Cohort prospective study, where we compared fourteen consecutive days period regarding in-hospital transmission. Each period met the 2 following conditions: 1) CPE carriers/infected admitted for at least 48h, 2) 80% of relative contact patients were screened at least twice...
February 10, 2018: Journal of Hospital Infection
Siew Mun Liew, Ganeswrei Rajasekaram, Savithri D Puthucheary, Kek Heng Chua
OBJECTIVES: The increasing incidence of carbapenem-resistant Pseudomonas aeruginosa along with the discovery of novel metallo-β-lactamases (MBLs) is of concern. In this study, the isolation of Malaysian MBL-producing P. aeruginosa clinical strains was investigated. METHODS: Fifty-three P. aeruginosa clinical strains were isolated from different patients in Sultanah Aminah Hospital, Johor Bahru, Malaysia in 2015. Antimicrobial susceptibility test was conducted. Minimum inhibitory concentrations (MICs) of imipenem and meropenem were determined by Etest...
February 9, 2018: Journal of Global Antimicrobial Resistance
Kayleigh E Mitchell, Jane F Turton, David H Lloyd
BACKGROUND: Acinetobacter species can exhibit widespread resistance to antimicrobial agents. They are already recognized as important nosocomial pathogens of humans, but are becoming increasingly recognized in opportunistic infections of animals. HYPOTHESIS/OBJECTIVES: This study aimed to determine whether Acinetobacter spp. are carried on skin of healthy dogs and, if present, to identify the species. ANIMALS: Forty dogs were sampled at veterinary practices and rescue centres...
February 11, 2018: Veterinary Dermatology
Yothin Teethaisong, Glyn Hobbs, Ismini Nakouti, Katie Evans, Griangsak Eumkeb
Reliable, simple and rapid methods for laboratory detection of carbapenemases are important for an appropriate antibiotic administration. A nitrocefin disc containing ertapenem for rapid screening of carbapenemase production among Enterobacteriaceae is developed in the present study. A total of 87 molecularly-confirmed Enterobacteriaceae including 31 carbapenemase producers and 56 non-carbapenemase producers, were tested with nitrocefin discs supplemented with and without ertapenem (20 μg/disc). Nitrocefin discs with ertapenem successfully discriminated all 31 carbapenemase and all non-carbapenemase producers within 30 minutes...
January 6, 2018: Diagnostic Microbiology and Infectious Disease
K R Nirupama, O R Vinodh Kumar, B S Pruthvishree, D K Sinha, M Senthil Murugan, Narayanan Krishnaswamy, B R Singh
OBJECTIVES: The aim of the study was to characterise the carbapenemase, extended spectrum beta -lactamase (ESBL) and Shiga toxin producing Escherichia coli isolated from farm piglets of India. METHODS: The faecal samples (n=741) from 10 organized pig farms, including non-diarrhoeic (n=546) and diarrhoeic (n=195) piglets were processed for isolation of carbapenem resistant and ESBL producing E. coli. RESULTS: A total of 27 and 243 isolates were phenotypically confirmed as carbapenem resistant and ESBL producers respectively...
February 3, 2018: Journal of Global Antimicrobial Resistance
Yafei Ye, Lijuan Xu, Yanping Han, Zhe Chen, Cailin Liu, Liang Ming
Carbapenemase-producing 'super bacteria', particularly NDM-1 and its variants, have become a major public health concern worldwide. The present study aimed to explore the molecular mechanism for carbapenem resistance of clinical Enterobacteriaceae isolates. Seventy-eight non-repeated Enterobacteriaceae strains resistant to any carbapenem were screened at the First Affiliated Hospital of Zhengzhou University (Zhengzhou, China) between December 2011 and December 2015. Outer membrane porin (OMP) proteins were detected using SDS-PAGE...
January 2018: Experimental and Therapeutic Medicine
João Botelho, Filipa Grosso, Sandra Quinteira, Michael Brilhante, Helena Ramos, Luísa Peixe
Objectives: Information on clonal lineages and genetic platforms involved in the mobilization of carbapenemases between Pseudomonas aeruginosa strains in Portugal is scarce. Here, we outline the genetic drivers contributing to the occurrence of blaVIM-2-producing P. aeruginosa over two decades. Methods: A collection of carbapenem-resistant P. aeruginosa clinical isolates (n = 263, 1995-2014) was screened for carbapenemase production by Blue-Carba and PCR. Antimicrobial susceptibility testing was performed according to EUCAST and clonal analysis by MLST...
January 23, 2018: Journal of Antimicrobial Chemotherapy
Zaineb Hamzaoui, Alain Ocampo-Sosa, Elaa Maamar, Marta Fernandez Martinez, Sana Ferjani, Samia Hammami, Sarra Harbaoui, Nathalie Genel, Guillaume Arlet, Mabrouka Saidani, Amine Slim, Ilhem Boutiba-Ben Boubaker, Luis Martinez-Martinez
OBJECTIVES: To describe clinical and molecular characteristics of an outbreak due to metallo-β-lactamases (MBLs) producing Klebsiella pneumoniae collected at Charles Nicolle Hospital of Tunis and to analyze the impact of outer membrane porin (OMP) loss on carbapenem resistance levels. METHODS: Between 2010 and 2015, 178 carbapenem-resistant Enterobacteriaceae were isolated. Screening for MBL production was performed using combined disk diffusion method, with imipenem and ethylene diamine tetraacetic acid (EDTA) as inhibitors...
January 26, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
Mathias W Pletz, Antje Wollny, Ute-Heike Dobermann, Jürgen Rödel, Svetlana Neubauer, Claudia Stein, Christian Brandt, Anita Hartung, Alexander Mellmann, Sabine Trommer, Birgit Edel, Vladimir Patchev, Oliwia Makarewicz, Jens Maschmann
Background: A foodborne outbreak of VIM carbapenemase-expressing Citrobacter freundii (CPC) occurred between February and June 2016 at a major university hospital in Germany. Methods: An explosive increase of CPC isolated from rectal swabs of patients during weekly routine screening led to the declaration of an outbreak. A hospital-wide prevalence screening was initiated as well as screening of all patients on admission and before transfer to another ward, and canteen staff, patient rooms, medical and kitchen inventory and food...
January 15, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
Yulia Rosa Saharman, Anis Karuniawati, Rudyanto Sedono, Dita Aditianingsih, Pratiwi Sudarmono, Wil H F Goessens, Corné H W Klaassen, Henri A Verbrugh, Juliëtte A Severin
Background: Carbapenem-nonsusceptible A. baumannii-calcoaceticus complex have emerged worldwide, but the epidemiology in Indonesian hospitals has not been studied. Methods: A prospective observational study was performed on the intensive care units (ICUs) of the national referral hospital in Jakarta-Indonesia, in 2013 and 2014. All consecutive adult patients admitted and hospitalized for >48 h in ICUs were included. Basic and clinical data at admission were recorded...
2018: Antimicrobial Resistance and Infection Control
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"