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Carbapenemase screening

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https://www.readbyqxmd.com/read/28642140/emergence-of-carbapenem-resistant-enterobacteriaceae-isolated-from-patients-in-a-university-hospital-in-saudi-arabia-epidemiology-clinical-profiles-and-outcomes
#1
Fawzia E Alotaibi, Elham E Bukhari, Maha M Al-Mohizea, Taghreed Hafiz, Eman Bin Essa, Yasmeen I AlTokhais
Carbapenemase-producing Enterobacteriaceae have been steadily spreading worldwide during the last decade. Nine patients were identified prospectively and were followed during their hospitalization course to identify the epidemiology, clinical profiles and outcomes. These patients had one or more cultures positive for a CRE isolate, contributing to a total of eleven positive cultures from various sites without including duplicates of isolates obtained from the same site. Isolates from these patients included five Klebseilla pneumoniae, three Escherichia coli, and one Enterobacter aerogenes...
June 19, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/28639165/molecular-characterization-of-intestinal-carriage-of-carbapenem-resistant-enterobacteriaceae-among-inpatients-at-two-iranian-university-hospitals-first-report-of-co-production-of-bla-ndm-7-and-bla-oxa-48
#2
H Solgi, F Badmasti, Z Aminzadeh, C G Giske, M Pourahmad, F Vaziri, S A Havaei, F Shahcheraghi
Gastrointestinal colonization of carbapenem-resistant Enterobacteriaceae (CRE) could serve as a reservoir for the transmission of these pathogens in the clinical setting. The aim of this study was to investigate the intestinal carriage of CRE and to analyze risk factors for CRE carriage. Rectal swabs were collected from 95 patients at two Iranian university hospitals. CRE screening was performed using selective media (CHROMagar and MacConkey agar). Polymerase chain reaction (PCR) was used to detect carbapenemase-encoding genes...
June 21, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28636666/molecular-and-microbiological-report-of-a-hospital-outbreak-of-ndm-1-carrying-enterobacteriaceae-in-mexico
#3
Paola Bocanegra-Ibarias, Elvira Garza-González, Rayo Morfín-Otero, Humberto Barrios, Licet Villarreal-Treviño, Eduardo Rodríguez-Noriega, Ulises Garza-Ramos, Santiago Petersen-Morfin, Jesus Silva-Sanchez
OBJECTIVES: To characterize the microbiological, molecular and epidemiological data of an outbreak of carbapenem-resistant Enterobacteriaceae (CRE) in a tertiary-care hospital in Mexico. METHODS: From September 2014 to July 2015, all CRE clinical isolates recovered during an outbreak in the Hospital Civil "Fray Antonio Alcalde" in Jalisco, Mexico were screened for antimicrobial susceptibility, carbapenemase production, carbapenemase-encoding genes, and plasmid profiles...
2017: PloS One
https://www.readbyqxmd.com/read/28630686/fecal-carriage-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-enterobacteriaceae-in-egyptian-patients-with-community-onset-gastrointestinal-complaints-a-hospital-based-cross-sectional-study
#4
H M Abdallah, N Alnaiemi, E A Reuland, B B Wintermans, A Koek, A M Abdelwahab, A Samy, K W Abdelsalam, C M J E Vandenbroucke-Grauls
OBJECTIVES: The aim of this study was to determine the prevalence of extended-spectrum β-lactamase (ESBL) and carbapenemase production among Enterobacteriaceae isolated from ambulatory patients with gastrointestinal complaints admitted to El-Ahrar General Hospital, Zagazig, Egypt in the period between January 2013 and May 2013. METHODS: One hundred and thirteen Enterobacteriaceae isolates were recovered from 100 consecutive Egyptian patients with community-onset gastrointestinal complaints...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28607010/comparison-of-two-phenotypic-algorithms-to-detect-carbapenemase-producing-enterobacteriaceae
#5
Laurent Dortet, Sandrine Bernabeu, Camille Gonzalez, Thierry Naas
A novel algorithm designed for the screening of carbapenemase producing Enterobactericeae (CPE), based on faropenem and temocillin disks was compared to that of the French Society of Microbiology (CA-SFM), which is based on ticarcillin-clavulanate, imipenem and temocillin disks. Both algorithms presented comparable negative predictive values (98.6% vs 97.5%) for the CPE screening among carbapenem non-susceptible Enterobacteriaceae However, since 46.2% (n=49) of the CPEs were correctly identified as OXA-48-like producers by the faropenem/temocillin-based algorithm, it significantly decreased the number of complementary tests needed (42...
June 12, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28602387/surveillance-of-carbapenemase-producing-enterobacteriaceae%C3%A2-in-the-indian-ocean-region-between-january-2010%C3%A2-and-december-2015
#6
A M Holman, J Allyn, G Miltgen, N Lugagne, N Traversier, S Picot, A Lignereux, C Oudin, O Belmonte, N Allou
BACKGROUND: The aim of this study was to trace the emergence of carbapenemase-producing Enterobacteriaceae (CPE) on Reunion Island, a French overseas territory well suited for the surveillance of CPE emergence in patients from the entire Indian Ocean Region. METHODS: This retrospective multicenter study was conducted on Reunion Island between 2010 and 2015. RESULTS: A total of 43 CPEs were isolated during the course of the study, in 36 patients (50% in the last year alone)...
June 8, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28600076/the-contribution-and-limits-of-clinical-specimens-for-the-screening-of-intestinal-multidrug-resistant-bacteria-in-view-of-laboratory-automation
#7
Geraud De Galassus Alix, Florence Cizeau, Aurélie Agathine, Cecilia Domrane, David Ducellier, Vincent Fihman, Jean-Winoc Decousser
The detection of multidrug-resistant bacteria carriers constitutes a race against time for infection preventionists. Alongside standard analysis for diagnosis purposes and rectal screening strategy, we tested a heavy-loaded selective method against 562 clinical specimens from 439 patients to detect extended-spectrum beta-lactamase-producing (ESBL) or carbapenemase-producing Enterobacteriaceae (CPE) and vancomycin-resistant enterococci (VRE). Our approach identified five more specimens positive for ESBL-producing Enterobacteriaceae than standard analysis, and 6 out of 9 known VRE/CPE carriers (three new CPE/VRE strains were also identified in this cohort)...
June 6, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28594634/dissemination-of-blaoxa-23-blaoxa-24-blaoxa-58-and-blandm-1-genes-of-acinetobacter-baumannii-isolates-from-four-tertiary-hospitals-in-thailand
#8
Udomluk Leungtongkam, Rapee Thummeepak, Suchada Wongprachan, Pollawat Thongsuk, Thawatchai Kitti, Kwanjai Ketwong, Chakkaphan Runcharoen, Narisara Chantratita, Sutthirat Sitthisak
Acinetobacter baumannii is a major threat to public health due to the emergence and dissemination of antibiotic-resistant strains. The purpose of this study was to determine the molecular epidemiology of antibiotic-resistant A. baumannii isolates collected from four tertiary hospitals in Thailand during the period November 2013-February 2015. We screened 339 A. baumannii, nonrepetitive clinical isolates to determine drug susceptibility. Among all isolates, we found that 7.9% was nondrug-resistant A. baumannii (NR-AB)...
June 8, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28562752/phenotypic-methods-for-screening-carbapenem-resistant-enterobacteriaceae-and-assessment-of-their-antimicrobial-susceptibility-profile
#9
Danielly da Costa Silva, Roberta Filipini Rampelotto, Vinícius Victor Lorenzoni, Silvana Oliveira Dos Santos, Juliana Damer, Manfredo Hörner, Rosmari Hörner
INTRODUCTION: In this study, we used phenotypic methods to screen carbapenem-resistant Enterobacteriaceae (CREs) and evaluated their antimicrobial sensitivity profile. METHODS: One hundred and seventy-eight CREs were isolated at a university hospital in south Brazil in a one-year period. Samples were assessed using disk diffusion tests with inhibitors of β-lactamases such as phenylboronic acid (AFB), cloxacillin (CLOXA), and ethylenediaminetetraacetic acid (EDTA)...
March 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28555403/dissemination-of-bla-oxa-370-gene-among-several-enterobacteriaceae-species-in-brazil
#10
C M Magagnin, F P Rozales, L Antochevis, L S Nunes, A S Martins, A L Barth, J M Sampaio, A P Zavascki
OXA-370 is a recently described OXA-48 variant that has only been described in a few Enterobacter spp. and Klebsiella pneumoniae isolates. The purpose of this study is to assess the prevalence of OXA-370-producing isolates in carbapenem-nonsusceptible Enterobacteriaceae recovered from 28 hospitals from Brazil. Real-time PCR was used to determine the presence of bla NDM-1, bla KPC-2, bla VIM-type, bla GES-type, bla OXA-48-like, and bla IMP-type genes. A total of 4,451 Enterobacteriaceae were screened. The gene bla OXA-48-like was detected in 74 (2...
May 30, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28537547/increasing-proportion-of-carbapenemase-producing-enterobacteriaceae-and-emergence-of-a-mcr-1-producer-through-a-multicentric-study-among-hospital-based-and-private-laboratories-in-belgium-from-september-to-november-2015
#11
Te Din Huang, Pierre Bogaerts, Catherine Berhin, Martin Hoebeke, Caroline Bauraing, Youri Glupczynski
Carbapenemase-producing Enterobacteriaceae (CPE) strains have been increasingly reported in Belgium. We aimed to determine the proportion of CPE among Enterobacteriaceae isolated from hospitalised patients and community outpatients in Belgium in 2015. For the hospitalised patients, the results were compared to a previous similar survey performed in the same hospitals in 2012. Twenty-four hospital-based and 10 private laboratories collected prospectively 200 non-duplicated Enterobacteriaceae isolates from clinical specimens...
May 11, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28511381/prevalence-of-carbapenem-resistant-non-fermenting-gram-negative-bacterial-infection-and-identification-of-carbapenemase-producing-nfgnb-isolates-by-simple-phenotypic-tests
#12
Jane Esther, Diego Edwin, Uma
INTRODUCTION: Non-Fermenting Gram negative bacilli (NFGNB) are emerging multi-drug resistant pathogens causing nosocomial infections. In recent years, carbapenem resistance in NFGNB has increased due to a variety of drug resistance mechanisms, the most common being production of carbapenemases. AIM: To detect carbapenemase and metallo-β-lactamase (MBL) production in NFGNBs by four phenotypic tests and to compare the various phenotypic methods for detection of carbapenemase and MBL production in nosocomial NFGNB isolates...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28502395/multicenter-evaluation-of-the-rapidec%C3%A2-carba-np-test-for-rapid-screening-of-carbapenemase-producing-enterobacteriaceae-and-gram-negative-nonfermenters-from-clinical-specimens
#13
Marco Coppi, Alberto Antonelli, Tommaso Giani, Teresa Spanu, Flora Marzia Liotti, Carla Fontana, Walter Mirandola, Raffaele Gargiulo, Agostino Barozzi, Carola Mauri, Luigi Principe, Gian Maria Rossolini
The rapid diagnosis of carbapenemase-producing (CP) bacteria is essential for the management of therapy and infection control. In this study, RAPIDEC® CARBA NP (RCNP) was evaluated for the rapid screening of CP Enterobacteriaceae, Acinetobacter baumannii complex, and Pseudomonas aeruginosa from clinical specimens collected at five Italian hospitals. Firstly, each site tested 20 well-characterized strains in a blinded fashion. Secondly, each center prospectively tested 25 isolates from blood cultures processed with a rapid workflow (6h after subculture) and 25 isolates from other specimens processed after an overnight culture...
April 27, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28498924/major-role-of-pkpqil-like-plasmids-in-the-early-dissemination-of-kpc-type-carbapenemases-in-the-uk
#14
M Doumith, J Findlay, H Hirani, K L Hopkins, D M Livermore, A Dodgson, N Woodford
Objectives: Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae were first seen in the UK in 2003 and have been increasingly reported since 2010, largely owing to an ongoing outbreak in North-West England. We examined the role of clonal spread and plasmid transmission in their emergence. Methods: : Isolates comprised KPC-positive K. pneumoniae ( n  =   33), Escherichia coli ( n  =   7) and Enterobacter spp. ( n  =   4) referred to the national reference laboratory between 2008 and 2010 from 17 UK centres, including three in North-West England...
May 11, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28487209/molecular-characterisation-of-carbapenemases-in-urban-pigeon-droppings-in-france-and-algeria
#15
Houda Morakchi, Lotfi Loucif, Djamila Gacemi-Kirane, Jean-Marc Rolain
OBJECTIVES: The main objective of this study was to detect the presence of carbapenemase-encoding genes in stool samples of urban pigeons. METHODS: Stool samples were collected from 73 pigeons in two Mediterranean cities, namely Marseille (France) and Annaba (Algeria). Faecal samples were screened by real-time PCR and standard PCR for the presence of carbapenemase-encoding genes. RESULTS: Carbapenem resistance genes were detected in 16 (21...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28486169/characterization-of-oxa-48-like-producing-enterobacteriaceae-isolated-from-river-water-in-algeria
#16
Rima Tafoukt, Abdelaziz Touati, Thongpan Leangapichart, Sofiane Bakour, Jean-Marc Rolain
The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
April 30, 2017: Water Research
https://www.readbyqxmd.com/read/28468855/simple-screening-for-carbapenemase-producing-enterobacteriaceae-by-moxalactam-susceptibility-testing
#17
Waka Imai, Masakazu Sasaki, Kotaro Aoki, Yoshikazu Ishii, Robert A Bonomo, Tse Hsien Koh, Hinako Murakami, Toshisuke Morita, Kazuhiro Tateda
The increase of carbapenemase producing Enterobacteriaceae (CPE) is a serious concern worldwide (1-7).….
May 3, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28458485/phenotypic-detection-of-extended-spectrum-beta-lactamase-and-carbapenemase-co-producing-clinical-isolates-from-two-tertiary-hospitals-in-kano-north-west-nigeria
#18
Yusuf Ibrahim, Yahaya Sani, Qabli Saleh, Algarni Saleh, Gbadamosi Hakeem
BACKGROUND: Continue rise in unprofessional use of antibiotics in our hospitals and communities is worrisome. A research study was therefore conducted to detect extended spectrum beta-lactamase (ESBL), carbapenemase, metallobeta lactamase and their co-production phenotypically from isolates obtained from patients admitted to or attending two tertiary hospitals in Kano, Nigeria. METHOD: A total of 248 isolates of Escherichia coli and Klebsiella pneumoniaewere screened phenotypically for ESBL production and carbapenemase production according to CLS1 2012 breakpoints using double disk synergy test and modified Hodge test (MHT) respectively...
January 2017: Ethiopian Journal of Health Sciences
https://www.readbyqxmd.com/read/28458051/emergence-and-nosocomial-spread-of-carbapenem-resistant-oxa-232-producing-klebsiella-pneumoniae-in-brunei-darussalam
#19
Muhd Haziq Fikry Abdul Momin, Apostolos Liakopoulos, Lynette M Phee, David W Wareham
OBJECTIVES: Carbapenem-resistant Enterobacteriaceae (CRE) are identified as a major global health concern. The success of CRE is facilitated by the emergence, acquisition and spread of successful clones carrying plasmid-encoded resistance genes. In this study, an outbreak of carbapenem-resistant Klebsiella pneumoniae (CRKP) infections in patients hospitalised in Brunei Darussalam was investigated. METHODS: Over a 3-month period (May-July 2015), five multidrug-resistant K...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28456489/evaluation-of-commercial-phenotypic-assays-for-the-detection-of-imp-or-new-delhi-metallo-%C3%AE-lactamase-producing-enterobacteriaceae-isolates-in-japan
#20
Daisuke Sakanashi, Makoto Kawachi, Yuki Uozumi, Mitsuru Nishio, Yuki Hara, Hiroyuki Suematsu, Mao Hagihara, Naoya Nishiyama, Nobuhiro Asai, Yusuke Koizumi, Yuka Yamagishi, Hiroshige Mikamo
OBJECTIVES: This study was designed to evaluate the sodium mercaptoacetic acid double disk synergy test (SMA-DDST), the Etest metallo-β-lactamase (MBL) MP/MPI (Etest MP/MPI), and the Mastdiscs ID Carbapenemase Detection Disc Set (MAST-CDS) for the detection of MBL-producing Enterobacteriaceae isolates in Japan. METHODS: Fifty-one clinical isolates and four reference strains were tested. These isolates included 40, 4, and 11 IMP-, New Delhi MBL (NDM)-, and non-MBL-producers, respectively...
April 26, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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