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Carbapenemase screening

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https://www.readbyqxmd.com/read/29141402/-epidemiology-of-the-carbapenemase-producing-enterobacteriaceae-spread-in-a-community-acute-hospital-and-a-non-acute-rehabilitation-hospital-in-madrid
#1
M López-Dosil, C Bischofberge, D Sáez, C García-Picazo
OBJECTIVE: In Spain, the overall prevalence of carbapenem-resistant Enterobacteriaceae (CRE) is increasing. We describe the epidemiological, clinical and microbiological characteristics features of patients with colonization or infection due to CRE in two hospitals in the north-west of Madrid during two years. One hospital was a community acute hospital and the second one was a non-acute rehabilitation hospital. METHODS: A total of 197 CPE isolates were detected during 2013-2014...
November 14, 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/29141051/molecular-and-epidemiological-characterization-of-carbapenemase-producing-enterobacteriaceae-in-norway-2007-to-2014
#2
Ørjan Samuelsen, Søren Overballe-Petersen, Jørgen Vildershøj Bjørnholt, Sylvain Brisse, Michel Doumith, Neil Woodford, Katie L Hopkins, Bettina Aasnæs, Bjørg Haldorsen, Arnfinn Sundsfjord
The prevalence of carbapenemase-producing Enterobacteriaceae (CPE) is increasing worldwide. Here we present associated patient data and molecular, epidemiological and phenotypic characteristics of all CPE isolates in Norway from 2007 to 2014 confirmed at the Norwegian National Advisory Unit on Detection of Antimicrobial Resistance. All confirmed CPE isolates were characterized pheno- and genotypically, including by whole genome sequencing (WGS). Patient data were reviewed retrospectively. In total 59 CPE isolates were identified from 53 patients...
2017: PloS One
https://www.readbyqxmd.com/read/29138113/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-encoding-genes-in-poultry-feces-from-algeria-and-marseille-france
#3
Chabou Selma, Leulmi Hamza, Davoust Bernard, Aouadi Atef, Jean-Marc Rolain
OBJECTIVE: The aim of this study was to evaluate the prevalence of extended-spectrum beta lactamase (ESBLs) and carbapenemase-encoding genes in poultry from Algeria and Marseille, France. MATERIALS AND METHODS: The samples consisted of feces from broilers from France and Algeria between 2014 and 2015. DNA extraction and quantitative PCR were performed on 833 fecal samples to screen for the presence of ESBLs (TEM, SHV, CTX-M) and carbapenemase-encoding genes (NDM, OXA48, OXA58)...
November 11, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29125052/prevalence-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-bloodstream-isolates-of-klebsiella-pneumoniae-in-a-tertiary-care-hospital
#4
Henrietta Abodakpi, Kai-Tai Chang, Ana María Sánchez Díaz, Rafael Cantón, Todd M Lasco, Katrina Chan, Amelia K Sofjan, Vincent H Tam
To improve prescribing of empiric therapy, the local molecular epidemiology of extended-spectrum beta-lactamases (ESBLs) and Klebsiella pneumoniae carbapenemases (KPCs) in bloodstream isolates of K. pneumoniae were evaluated. Isolates resistant to third generation cephalosporins were screened phenotypically for ESBLs and carbapenemases, and subsequently confirmed by PCR for the presence of ESBL (blaTEM, blaSHV and blaCTX-M) and carbapenemase (blaKPC, blaVIM, blaNDM and blaOXA-48) genes. Hydrolytic activity (functional gene expression) was quantified using a nitrocefin degradation assay and correlated to ceftazidime or meropenem MIC...
November 10, 2017: Journal of Chemotherapy
https://www.readbyqxmd.com/read/29115680/mastdiscs-combi-carba-plus-a-simple-discrimination-method-for-carbapenemase-producing-enterobacteriaceae-including-oxa-48-type-producers
#5
Yusuke Ohsaki, Ryoichi Kubo, Jonathan Hobson, Mya Davies, Shunsuke Osaka, Wataru Hayashi, Yui Taniguchi, Shota Koide, Yukiko Nagano, Noriyuki Nagano
Not only detection of carbapenemases but also identification of their types accurately and rapidly in Enterobacteriaceae is still a major challenge for clinical laboratories in order to prevent the intrusion and the transmission of carbapenemase-producing Enterobacteriaceae. This study aimed to evaluate the performance of the MASTDISCS® combi Carba plus disc system in the identification of different carbapenemase types, including OXA-48-type carbapenemase for which no specific enzyme inhibitors have so far been available...
November 8, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/29108951/automated-time-lapse-microscopy-a-novel-method-for-screening-of-antibiotic-combination-effects-against-multidrug-resistant-gram-negative-bacteria
#6
Wanchana Ungphakorn, Pernilla Lagerbäck, Elisabet I Nielsen, Thomas Tängdén
OBJECTIVES: Antibiotic combinations are often used for carbapenemase-producing Enterobacteriaceae (CPE) but more data are needed on the optimal selection of drugs. This study aimed to evaluate the feasibility of a novel automated method based on time-lapse microscopy (the oCelloScope, Philips BioCell A/S, Allerød, Denmark) to determine in vitro combination effects against CPE and to discuss advantages and limitations of the oCelloScope in relation to standard methods. METHODS: Four Klebsiella pneumoniae and two Escherichia coli were exposed to colistin, meropenem, rifampin and tigecycline, alone and in combination...
November 3, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29107607/emergence-and-mechanism-of-carbapenem-resistant-escherichia-coli-in-henan-china-2014
#7
Wen-Juan Liang, Hui-Ying Liu, Guang-Cai Duan, Yong-Xin Zhao, Shuai-Yin Chen, Hai-Yan Yang, Yuan-Lin Xi
The emergence and dissemination of carbapenem-resistant Escherichia coli (E. coli) strains is a main risk for global public health, but little is known of carbapenemase producing E. coli in Henan, China. The study was undertaken to investigate the prevalence and mechanism of carbapenem-resistant E. coli strains in a hospital in Xinxiang, Henan, China, 2014. A total of 5 carbapenemase-producing E. coli strains were screened from 1014 isolates. We found that they were all resistant to meropenem and imipenem. Amikacin showed the best sensitivity, with gentamicin coming up next...
October 26, 2017: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/29107415/enhanced-detection-of-carbapenemase-producing-enterobacteriaceae-by-an-optimized-phenol-red-assay
#8
Jeremy Surre, Isabelle Canard, Pierrot Bourne-Branchu, Estelle Courbiere, Christine Franceschi, Sonia Chatellier, Alex van Belkum, Mahendrasingh Ramjeet
Screening for the detection of carbapenemase-producing bacteria still encounters issues related to workflow, limit of detection, or qualitative interpretation. We developed a spectrophotometry-based version of the Carba NP phenol red assay (Nordmann et al., 2012) in a microtiter plate format, compatible with low bacterial cell counts. We were able to detect highly active carbapenemases such as KPC and IMP in 30min. A wider range of carbapenemases including OXA-48 were detected using higher inocula, still being competitive compared with currently available phenol red assays...
September 18, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29102775/blandm-1-producing-multidrug-resistant-escherichia-coli-isolated-from-a-companion-dog-in-china
#9
Lanqing Cui, Lei Lei, Yuan Lv, Rongmin Zhang, Xiaoyu Liu, Mei Li, Fan Zhang, Yang Wang
OBJECTIVES: This sudy aimed to characterize a blaNDM-1-producing Escherichia coli from a companion dog. METHODS: This strain was isolated from a surveillance study of carbapenem-resistant Gram-negative bacteria and the specie was identified by API 20E system and MALDI-TOF MS. MICs of various antimicrobial agents were determined by agar dilution. Multilocus sequence typing (MLST) and various carbapenemase genes including blaNDM, blaIMP, blaVIM, blaSME, blaIMI, blaKPC, blaGES, blaSPM, blaGIM, blaNMC, blaDIM, blaSIM, blaOXA-23, blaOXA-24, blaOXA-48, blaOXA-51 and blaOXA-58 were screened by PCR...
November 1, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29074053/optimal-detection-of-carbapenemase-producing-enterobacteriaceae-from-rectal-samples-a-role-for-enrichment
#10
H Ciesielczuk, L M Phee, H Dolphin, M Wilks, B P Cherian, D W Wareham
BACKGROUND: Successful laboratory detection of carbapenemase-producing Enterobacteriaceae (CPE) in patient surveillance samples is a diagnostic challenge. In the absence of a gold standard for screening rectal swabs for CPE many phenotypic, genotypic, culture and non-culture based assays have been proposed for identifying these bacteria. AIM: To develop and optimise a CPE screening protocol capable of identifying all commonly encountered CPE, including those producing OXA-48-like carbapenemases...
October 23, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29063891/identification-of-carbapenemase-mediated-resistance-among-enterobacteriaceae-bloodstream-isolates-a-molecular-study-from-india
#11
Srujana Mohanty, Mittal Gajanand, Rajni Gaind
Acquired resistance in carbapenem-resistant Enterobacteriaceae (CRE) conferred by carbapenemases is a major concern worldwide. Consecutive, non-duplicate isolates of Escherichia coli (EC) and Klebsiella pneumoniae from clinically diagnosed bloodstream infections were screened for the presence of carbapenem resistance by standard disk-diffusion method and minimum inhibitory concentration breakpoints using the Clinical and Laboratory Standards Institute guidelines. Carbapenemase-encoding genes were amplified by polymerase chain reaction...
July 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29056446/evaluation-of-the-rapid-biochemical-%C3%AE-carba%C3%A2-test-for-detection-of-carbapenemase-producing-gram-negative-bacteria
#12
Vladimira Hinić, Josiane Reist, Adrian Egli
We evaluated a rapid biochemical screening test β-CARBA™ for detection of Gram-negative carbapenemase producers. The sensitivity of the test after 30min incubation was 98.2%, but increased to 100% after 1h. β-CARBA™ proved reliable for carbapenemase screening in Acinetobacter spp. for which currently no commercial phenotypic assays are available.
October 19, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29051750/epidemiology-of-extended-spectrum-%C3%AE-lactamase-producing-e-coli-and-vancomycin-resistant-enterococci-in-the-northern-dutch-german-cross-border-region
#13
Xuewei Zhou, Silvia García-Cobos, Gijs J H M Ruijs, Greetje A Kampinga, Jan P Arends, Dirk M Borst, Lieke V Möller, Nicole D Holman, Theo A Schuurs, Lesla E Bruijnesteijn van Coppenraet, Jan F Weel, Jan H van Zeijl, Robin Köck, John W A Rossen, Alexander W Friedrich
Objectives: To reveal the prevalence and epidemiology of extended-spectrum β-lactamase (ESBL)- and/or plasmid AmpC (pAmpC)- and carbapenemase (CP) producing Enterobacteriaceae and vancomycin-resistant enterococci (VRE) across the Northern Dutch-German border region. Methods: A point-prevalence study on ESBL/pAmpC/CP producing Enterobacteriaceae and VRE was carried out in hospitalized patients in the Northern Netherlands (n = 445, 2012-2013) and Germany (n = 242, 2012). Healthy individuals from the Dutch community (n = 400, 2010-2012) were also screened...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29040585/emergence-of-mcr-1-and-carbapenemase-genes-in-hospital-sewage-water-in-beijing-china
#14
Longyang Jin, Ruobing Wang, Xiaojuan Wang, Qi Wang, Yawei Zhang, Yuyao Yin, Hui Wang
Objectives: This study identified and characterized mcr-1-positive Enterobacteriaceae (MCRPE) and carbapenemase-producing Enterobacteriaceae (CPE) in hospital sewage water. Methods: Influent and effluent sewage samples were collected from five tertiary hospitals in Beijing in December 2016. Samples were screened for MCRPE and CPE using antibiotic selection media. Results were confirmed by PCR amplification of β-lactamase and colistin resistance (mcr-1 and mcr-2) genes and by sequencing...
October 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29039717/high-prevalence-of-multidrug-resistant-bacteria-in-libyan-war-casualties-admitted-to-a-tertiary-care-hospital-germany
#15
Benedikt Lohr, Yvonne Pfeifer, Ursel Heudorf, Christoph Rangger, Douglas E Norris, Klaus-Peter Hunfeld
The ongoing Libyan conflict constantly causes victims among the military and civilian population. Cross-border transfer of patients represents a high risk of introducing multidrug-resistant organisms (MDROs), for example, methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococci, and carbapenem-resistant gram-negative organisms (CROs), into the country of destination. This study assessed the MDRO status in Libyan war casualties (n = 67) admitted to Northwest Medical Centre in Frankfurt/Main, Germany, from August 2016 till January 2017...
October 17, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29039274/different-escherichia-coli-b2-st131-clades-b-and-c-producing-extended-spectrum-%C3%AE-lactamases-esbl-colonizing-residents-of-portuguese-nursing-homes
#16
C Rodrigues, E Machado, S Fernandes, L Peixe, Â Novais
ESBL-producing Enterobacteriaceae and particularly Escherichia coli ST131 isolates producing CTX-M enzymes are commonly found colonizing the intestine of nursing home (NH) residents, but ST131 subclonal structure has been scarcely explored in this vulnerable population. Our goal was to perform a pilot study to assess the faecal carriage rate and epidemiological features of ESBL- and/or carbapenemase-producing Enterobacteriaceae (ESBL-E and CPE, respectively) among NH residents. For this purpose, faecal samples from residents at 4 different NHs in the North of Portugal (representing 9·5% of the residents' population, July 2014) were screened for ESBL-E and/or CPE by phenotypic and genotypic methods...
October 17, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/29029114/carbapenem-resistant-citrobacter-spp-isolated-in-spain-from-2013-to-2015-produced-a-variety-of-carbapenemases-including-vim-1-oxa-48-kpc-2-ndm-1-and-vim-2
#17
David M Arana, Adriana Ortega, Eva González-Barberá, Noelia Lara, Verónica Bautista, Dolores Gómez-Ruíz, David Sáez, Sara Fernández-Romero, Belén Aracil, María Pérez-Vázquez, José Campos, Jesús Oteo
Objectives: There is little information about carbapenemase-producing (CP) Citrobacter spp. We studied the molecular epidemiology and microbiological features of CP Citrobacter spp. isolates collected in Spain (2013-15). Methods: In total, 119 isolates suspected of being CP by the EUCAST screening cut-off values were analysed. Carbapenemases and ESBLs were characterized using PCR and sequencing. The genetic relationship among Citrobacter freundii isolates was studied by PFGE...
September 27, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29022829/migratory-white-stork-ciconia-ciconia-a-potential-vector-of-the-oxa-48-producing-escherichia-coli-st38-clone-in-algeria
#18
Amira Bouaziz, Lotfi Loucif, Ammar Ayachi, Karima Guehaz, Esma Bendjama, Jean-Marc Rolain
The emergence of carbapenemase-producing Enterobacteriaceae is of great concern to public health worldwide. The aim of this study was to screen for the presence of carbapenemase-producing Enterobacteriaceae in white stork (Ciconia ciconia) migratory bird stools, and to investigate their molecular support on β-lactamase production. In March 2015, 32 fecal samples of white stork were collected in the Commune of El Madher Wilaya de Batna, in eastern Algeria. Samples were subjected to selective isolation of carbapenem-resistant Enterobacteriaceae...
October 12, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28994446/colonization-of-residents-and-staff-of-an-italian-long-term-care-facility-and-an-adjacent-acute-care-hospital-geriatric-unit-by-multidrug-resistant-bacteria
#19
Albert March, Richard Aschbacher, Ferisa Sleghel, Gertrud Soelva, Malgorzata Kaczor, Roberta Migliavacca, Aurora Piazza, Vittoria Mattioni Marchetti, Laura Pagani, Katia Scalzo, Valentina Pasquetto, Elisabetta Pagani
In 2016, we undertook a point prevalence screening study for Enterobacteriaceae with extended-spectrum β-lactamases (ESBLs), high-level AmpC cephalosporinases and carbapenemases, and also methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin resistant enterococci (VRE) in a long-term care facility (LTCF) and the associated acute care hospital geriatric unit in Bolzano, Northern Italy. Urine samples and rectal, inguinal, oropharyngeal and nasal swabs were plated on selective agars. Demographic data were collected...
October 10, 2017: New Microbiologica
https://www.readbyqxmd.com/read/28980084/comparison-of-maldi-tof-ms-with-the-rapidec-carba-np-test-for-the-detection-of-carbapenemase-producing-enterobacteriaceae
#20
M Choquet, R Guiheneuf, S Castelain, V Cattoir, M Auzou, E Pluquet, V Decroix
Although carbapenemase-producing Enterobacteriaceae (CPE) have become a serious public health issue, their detection remains challenging. The aim of this study was to implement a test based on imipenem hydrolysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS), using 65 strains producing or not a carbapenemase. Then, we compared its performance to that of the Rapidec Carba NP test using 20 additional strains. The MS-based test effectively discriminated between CPE and other non-carbapenem-susceptible strains compared to the Rapidec Carba NP test (sensitivity 100% and 92%, specificity 94% and 92%, respectively)...
October 4, 2017: European Journal of Clinical Microbiology & Infectious Diseases
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