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Carbapenemase screening

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https://www.readbyqxmd.com/read/29775789/e-coli-encoding-blandm-5-associated-with-community-acquired-uti-cases-with-unusual-mic-creep-like-phenomenon-against-imipenem
#1
Varsha Rani Gajamer, Amitabha Bhattacharjee, Deepjyoti Paul, Chandrayee Deshamukhya, Ashish Kr Singh, Nilu Pradhan, Hare Krishna Tiwari
OBJECTIVES: Carbapenemase-producing Escherichia coli are of major clinical concern. The present study aimed to identify NDM-5 producing E.coli associated with community-acquired urinary tract infection, co-harboring ESBL genes and a pattern of imipenem MIC creep. METHODS: A total of 973 urine samples were collected from females of age between 18-49 diagnosed with UTI. Isolates were identified by standard microbiological procedures. Presence of blaNDM and ESBL genes was determined by PCR assay and sequencing...
May 15, 2018: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/29751417/occurrence-and-characteristics-of-extended-spectrum-%C3%AE-lactamase-and-carbapenemase-producing-bacteria-from-hospital-effluents-in-singapore
#2
Laurence Haller, Hongjie Chen, Charmaine Ng, Thai Hoang Le, Tse Hsien Koh, Timothy Barkham, Mark Sobsey, Karina Yew-Hoong Gin
One of the most important resistance mechanisms in Gram-negative bacteria today is the production of enzymes causing resistance to cephalosporin and carbapenem antibiotics. The spread of extended-spectrum β-lactamases (ESBL)- and carbapenemase- producing Gram-negative bacteria is an emerging global public health problem. The aim of the present study was to (i) assess the prevalence of carbapenem-resistant bacteria (CRB) and ESBL-producing strains in sewage effluents from two major hospitals in Singapore, (ii) characterize the isolated strains and (iii) identify some of the ESBL and carbapenemase genes responsible for the resistance...
February 15, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29709404/microbiology-of-french-military-casualties-repatriated-from-overseas-for-an-open-traumatic-injury
#3
S Larréché, A Bousquet, C Soler, C Mac Nab, D de Briel, D Delaune, C Bigaillon, P Pasquier, C Dubost, T Demoures, B Malgras, S Ausset, S de Rudnicki, T Leclerc, B de Loynes, S Bonnet, N Mocellin, C Ficko, R Haus, O Hersan, S Rigal, A Mérens
BACKGROUND: This study aimed to describe the microbiological epidemiology of repatriated French soldiers with an open traumatic injury, and to measure the proportion of multidrug-resistant bacteria (MDRB). METHODS: Retrospective study including all French soldiers repatriated in 2011 and 2012 in Parisian military hospitals for open traumatic injury. Results of clinical samples and MDRB screening were collected. The antibiotic susceptibility was assessed using the agar disk diffusion method...
April 27, 2018: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/29707661/vim-klebsiella-oxytoca-outbreak-in-a-neonatal-intensive-care-unit-this-time-it-wasn-t-the-drain
#4
R Herruzo, G Ruiz, S Gallego, J Diez, A Sarria, F Omeñaca
Objective: We describe an outbreak of VIM-carbapenemase Klebsiella oxytoca (VIM-Kox) in a NICU. Materials and methods: Prospective Epidemiological Surveillance:Systematically (weekly screening cultures) or on admission, if the patient had a history of previous colonization by VIM-Kox.Clinical cultures, done if infection was suspected.Other possible microorganism sources were investigated: their mothers (rectal microbiota), milk packages and preparation apparata in the lactodietary section, echocardiagram transductors, cribs, the sinks (faucets and drains), washing bowls, etc...
December 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/29692579/molecular-characterization-and-antimicrobial-susceptibility-profile-of-new-delhi-metallo-beta-lactamase-1-producing-escherichia-coli-among-hospitalized-patients
#5
Anjali Agarwal, Jyoti Srivastava, Ujjwal Maheshwari, Mohd Iftikhar
BACKGROUND: Carbapenemase-producing microorganisms are becoming a major concern among hospital-acquired infections. There is also increased multidrug resistance seen among these isolates. AIMS: We have conducted this study to determine the prevalence of New Delhi metallo-beta-lactamase-1 (NDM-1) gene-producing Escherichia coli among hospitalized patients in a tertiary care hospital in Northern India. SETTINGS AND DESIGN: The study was conducted in the Department of Microbiology with the tertiary care hospital settings...
April 2018: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/29688801/genomic-characterization-of-nonclonal-mcr-1-positive-multidrug-resistant-klebsiella-pneumoniae-from-clinical-samples-in-thailand
#6
Apichai Srijan, Katie R Margulieux, Sirigade Ruekit, Erik Snesrud, Rosslyn Maybank, Oralak Serichantalergs, Rosarin Kormanee, Prawet Sukhchat, Jossin Sriyabhaya, Mary Hinkle, John M Crawford, Patrick McGann, Brett E Swierczewski
Multidrug-resistant Klebsiella pneumoniae strains are one of the most prevalent causes of nosocomial infections and pose an increasingly dangerous public health threat. The lack of remaining treatment options has resulted in the utilization of older drug classes, including colistin. As a drug of last resort, the discovery of plasmid-mediated colistin resistance by mcr-1 denotes the potential development of pandrug-resistant bacterial pathogens. To address the emergence of the mcr-1 gene, 118 gram-negative Enterobacteriaceae isolated from clinical samples collected at Queen Sirikit Naval Hospital in Chonburi, Thailand were screened for colistin resistance using automated antimicrobial susceptibility testing and conventional PCR screening...
April 24, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29684930/-prevention-and-detection-of-multidrug-resistant-bacteria
#7
Simone Scheithauer, Martin Kaase
Patients colonized or infected by multidrug-resistant bacteria (MDR) are entitled to the same medical treatment as other patients and infection control measures based on the current evidence are not in conflict with this aim. Recent studies indicate that the correct sampling technique is of utmost importance when screening for MRSA and usage of certain swabs might reduce the sensitivity considerably. Despite improvements in diagnostic strategies for direct MRSA detection by PCR, false positive results cannot be totally excluded...
May 2018: Deutsche Medizinische Wochenschrift
https://www.readbyqxmd.com/read/29682477/identification-of-extended-spectrum-beta-lactamases-ampc-and-carbapenemase-production-among-isolates-of-escherichia-coli-in-north-indian-tertiary-care-centre
#8
Uma Chaudhary, Shipra Agarwal, Kausalya Raghuraman
Introduction: Identification of Extended spectrum beta lactamases (ESBL), AmpC production and carbapenemase production among isolates of Escherichia coli , helps clinician to rationalize the choice of antibiotics. However, there is a lack of simple and effective method for simultaneous identification of these beta lactamases. Aim: To determine the concurrent production of beta lactamases using twelve disc method on E. coli isolates. Materials and Methods: A total of 200 multidrug resistant E...
April 2018: Avicenna Journal of Medicine
https://www.readbyqxmd.com/read/29674034/a-voltammetric-test-for-the-rapid-discrimination-of-%C3%AE-lactamase-producing-enterobacteriaceae-in-blood-cultures
#9
Laetitia Betelli, Catherine Neuwirth, Sébastien Solanas, Benoît Chantemesse, Fabienne Vienney, Alain Hartmann, Murielle Rochelet
The accurate identification of β-lactamases produced by Enterobacteriaceae is a major challenge in clinical laboratories in order to optimize antimicrobial treatment and patient care. We describe here a rapid voltammetric-based method to detect and to discriminate β-lactamase activity in Enterobacteriaceae i.e., penicillinase, cephalosporinase (inducible or overproduced), extended-spectrum beta-lactamase and carbapenemase producers. After a 2-h growth step of the sample under three separate conditions: 1) LB (Luria-Bertani) medium, 2) LB supplemented with 4 μg/mL cefotaxime and 3) LB supplemented with 4 μg/mL cefotaxime and 100 μg/mL potassium clavulanate, the β-lactamase activity was measured by incubating a 0...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29664716/identification-of-carbapenem-resistant-pseudomonas-aeruginosa-in-selected-hospitals-of-the-gulf-cooperation-council-states-dominance-of-high-risk-clones-in-the-region
#10
Hosam M Zowawi, Melanie W Syrmis, Timothy J Kidd, Hanan H Balkhy, Timothy R Walsh, Sameera M Al Johani, Reem Y Al Jindan, Mubarak Alfaresi, Emad Ibrahim, Amina Al-Jardani, Jameela Al Salman, Ali A Dashti, Hanna E Sidjabat, Omar Baz, Ella Trembizki, David M Whiley, David L Paterson
PURPOSE: The molecular epidemiology and resistance mechanisms of carbapenem-resistant Pseudomonas aeruginosa (CRPA) were determined in hospitals in the countries of the Gulf Cooperation Council (GCC), namely, Saudi Arabia, the United Arab Emirates, Oman, Qatar, Bahrain and Kuwait. METHODOLOGY: Isolates were screened for common carbapenem-resistance genes by PCR. Relatedness between isolates was assessed using previously described genotyping methods: an informative-single nucleotide polymorphism MassARRAY iPLEX assay (iPLEX20SNP) and the enterobacterial repetitive intergenic consensus (ERIC)-PCR assay, with selected isolates being subjected to multilocus sequence typing (MLST)...
April 17, 2018: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/29642825/-investigation-of-carbapenemase-genes-and-molecular-epidemiology-of-enterobacteriaceae-strains-isolated-between-2010-2014-in-a-university-hospitals
#11
Hüseyin Haydar Kutlu, Ebru Us, Alper Tekeli
The worldwide spread of carbapenemase producing Enterobacteriaceae isolates has become a major threat of public health. This worrisome situation leads the development of new methods for carbapenemase screening, detection, prevention of spread and epidemiological data collection as mandatory. In this study, it was aimed to investigate existence and distribution of carbapenemase-encoding genes (CEGs) among carbapenem-resistant Enterobacteriaceae isolated from various clinical samples in Ankara University Faculty of Medicine, Ibni Sina Hospital, Central Microbiology Laboratory between June 2010-May 2014 and detect their clonal relationship...
January 2018: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/29621209/vital-signs-containment-of-novel-multidrug-resistant-organisms-and-resistance-mechanisms-united-states-2006-2017
#12
Kate Russell Woodworth, Maroya Spalding Walters, Lindsey M Weiner, Jonathan Edwards, Allison C Brown, Jennifer Y Huang, Sarah Malik, Rachel B Slayton, Prabasaj Paul, Catherine Capers, Marion A Kainer, Nancy Wilde, Alicia Shugart, Garrett Mahon, Alexander J Kallen, Jean Patel, L Clifford McDonald, Arjun Srinivasan, Michael Craig, Denise M Cardo
BACKGROUND: Approaches to controlling emerging antibiotic resistance in health care settings have evolved over time. When resistance to broad-spectrum antimicrobials mediated by extended-spectrum β-lactamases (ESBLs) arose in the 1980s, targeted interventions to slow spread were not widely promoted. However, when Enterobacteriaceae with carbapenemases that confer resistance to carbapenem antibiotics emerged, directed control efforts were recommended. These distinct approaches could have resulted in differences in spread of these two pathogens...
April 6, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29596903/high-prevalence-of-16s-rrna-methyltransferases-among-carbapenemase-producing-enterobacteriaceae-in-the-uk-ireland
#13
Emma Taylor, Shiranee Sriskandan, Neil Woodford, Katie L Hopkins
The emergence of 16S rRNA methyltransferases (16S RMTases) worldwide is a growing concern due to their ability to confer high-level resistance (MICs >256 mg/L) to all clinically-relevant aminoglycosides. As the occurrence of 16S RMTases in the United Kingdom has not been investigated to date, we screened 806 Enterobacteriaceae isolates displaying high-level aminoglycoside resistance (amikacin, gentamicin and tobramycin MICs ≥64, ≥32 and ≥32 mg/L, respectively) for 16S RMTases either by analysing whole-genome sequence (WGS) data (which were available for 449 isolates) or by PCR...
March 26, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29579235/duodenoscope-related-outbreak-of-a-carbapenem-resistant-klebsiella-pneumoniae-identified-using-advanced-molecular-diagnostics
#14
Romney M Humphries, Shuan Yang, Stephen Kim, Venkatara Raman Muthusamy, Dana Russell, Alisa M Trout, Teresa Zaroda, Quen J Cheng, Grace Aldrovandi, Daniel Zachary Uslan, Peera Hemarajata, Zachary Aaron Rubin
Background: Carbapenem-resistant Klebsiella pneumoniae infections are increasingly prevalent in North American hospitals. We describe an outbreak of carbapenem-resistant K. pneumoniae containing the blaOXA-232 gene transmitted by contaminated duodenoscopes during endoscopic retrograde cholangiopancreatography (ERCP) procedures. Methods: An outbreak investigation was performed when 9 patients with blaOXA-232 carbapenem-resistant K. pneumoniae infections were identified at a tertiary care hospital...
October 1, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29575700/antimicrobial-resistant-enterobacteriaceae-recovered-from-companion-animal-and-livestock-environments
#15
R J Adams, S S Kim, D F Mollenkopf, D A Mathys, G M Schuenemann, J B Daniels, T E Wittum
Antimicrobial-resistant bacteria represent an important concern impacting both veterinary medicine and public health. The rising prevalence of extended-spectrum beta-lactamase (ESBL), AmpC beta-lactamase, carbapenemase (CRE) and fluoroquinolone-resistant Enterobacteriaceae continually decreases the efficiency of clinically important antibiotics. Moreover, the potential for zoonotic transmission of antibiotic-resistant enteric bacteria increases the risk to public health. Our objective was to estimate the prevalence of specific antibiotic-resistant bacteria on human contact surfaces in various animal environments...
March 25, 2018: Zoonoses and Public Health
https://www.readbyqxmd.com/read/29548525/evaluation-of-the-carbapenem-detection-set%C3%A2-for-the-detection-and-characterization-of-carbapenemase-producing-enterobacteriaceae
#16
Laurent Dortet, Sandrine Bernabeu, Camille Gonzalez, Thierry Naas
The objective of this study was to assess the performance of the Carbapenemase Detection Set™ (CDS; Mast Diagnostics) in association with i) the EUCAST meropenem screening cut-off and ii) the faropenem-temocillin algorithm (FTa) for the screening of carbapenemase-producing Enterobacteriaceae (CPE). A total of 200 well-characterized enterobacterial isolates with reduced susceptibility to at least one carbapenem including 63 non-CPEs and 137 CPEs belonging to different Ambler classes were initially screened for CPEs using i) the EUCAST meropenem cut-off (diameter <25 mm) and ii) the FTa...
February 17, 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29531581/evaluation-of-combined-use-of-temocillin-disk-and-mastdisks-inhibitor-combination-set-against-polymerase-chain-reaction-for-detection-of-carbapenem-resistant-enterobacteriaceae
#17
Omnia A Shaker, Howayda E Gomaa, Shereen A ElMasry, Rania M Abdel Halim, Amany H Abdelrahman, Jackline S Kamal
AIM: To evaluate the diagnostic performance of MDI and temocillin disk (30 μg) for detection of carbapenem-resistant Enterobacteriaceae in comparison to real-time PCR. MATERIAL AND METHODS: Fifty specimens submitted to the Microbiology Laboratory of Ain Shams University Hospitals and showed resistance to carbapenem drugs through routine culture and susceptibility testing, were assessed by both temocillin disk (30 μg) and MDI set to detect carbapenem-resistant Enterobacteriaceae ...
February 15, 2018: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29530741/an-evaluation-of-a-toolkit-for-the-early-detection-management-and-control-of-carbapenemase-producing-enterobacteriaceae-a-cross-sectional-survey-of-nhs-acute-trusts-in-england
#18
Caroline M Coope, Neville Q Verlander, Annegret Schneider, Susan Hopkins, William Welfare, Alan P Johnson, Bharat Patel, Isabel Oliver
BACKGROUND: Following hospital outbreaks of carbapenemase-producing Enterobacteriaceae (CPE), Public Health England published a toolkit in December 2013 to promote the early detection, management and control of CPE colonisation and infection in acute hospital settings. AIM: This evaluation aimed to examine awareness, uptake, implementation and usefulness of the CPE toolkit and identify potential barriers and facilitators to its adoption in order to inform future guidance...
March 9, 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29527303/colonization-of-long-term-care-facility-residents-in-three-italian-provinces-by-multidrug-resistant-bacteria
#19
Elisabetta Nucleo, Mariasofia Caltagirone, Vittoria Mattioni Marchetti, Roberto D'Angelo, Elena Fogato, Massimo Confalonieri, Camilla Reboli, Albert March, Ferisa Sleghel, Gertrud Soelva, Elisabetta Pagani, Richard Aschbacher, Roberta Migliavacca, Laura Pagani
Background: Rationale and aims of the study were to compare colonization frequencies with MDR bacteria isolated from LTCF residents in three different Northern Italian regions, to investigate risk factors for colonization and the genotypic characteristics of isolates. The screening included Enterobacteriaceae expressing extended-spectrum β-lactamases (ESβLs) and high-level AmpC cephalosporinases, carbapenemase-producing Enterobacteriaceae , Pseudomonas aeruginosa or Acinetobacter baumannii , methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE)...
2018: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/29510189/in-vitro-activity-of-meropenem-vaborbactam-and-characterization-of-carbapenem-resistance-mechanisms-among-carbapenem-resistant-enterobacteriaceae-from-the-2015-meropenem-vaborbactam-surveillance-program
#20
Michael A Pfaller, Michael D Huband, Rodrigo E Mendes, Robert K Flamm, Mariana Castanheira
We evaluated meropenem-vaborbactam activity against 11,559 Enterobacteriaceae isolates, including 330 carbapenem-resistant phenotypes (CRE) and carbapenemase genotypes collected worldwide during 2015. Meropenem-vaborbactam (inhibitor at 8 mg/L) and comparators were susceptibility tested using reference broth microdilution methods. CRE isolates were screened for the presence of genes encoding carbapenemases and 292 (88.5%) of the CRE isolates carried these resistance genes. A total of 209 (63.3% of the CRE; 1...
March 3, 2018: International Journal of Antimicrobial Agents
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