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Carbapenemase screening

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https://www.readbyqxmd.com/read/28444620/environmental-pollution-with-antimicrobial-agents-from-bulk-drug-manufacturing-industries-in-hyderabad-south-india-is-associated-with-dissemination-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pathogens
#1
Christoph Lübbert, Christian Baars, Anil Dayakar, Norman Lippmann, Arne C Rodloff, Martina Kinzig, Fritz Sörgel
PURPOSE: High antibiotic and antifungal concentrations in wastewater from anti-infective drug production may exert selection pressure for multidrug-resistant (MDR) pathogens. We investigated the environmental presence of active pharmaceutical ingredients and their association with MDR Gram-negative bacteria in Hyderabad, South India, a major production area for the global bulk drug market. METHODS: From Nov 19 to 28, 2016, water samples were collected from the direct environment of bulk drug manufacturing facilities, the vicinity of two sewage treatment plants, the Musi River, and habitats in Hyderabad and nearby villages...
April 26, 2017: Infection
https://www.readbyqxmd.com/read/28442306/evaluation-of-a-real-time-pcr-assay-for-rectal-screening-of-oxa-48-producing-enterobacteriaceae-in-a-general-intensive-care-unit-of-an-endemic-hospital
#2
J Fernández, S A Cunningham, A Fernández-Verdugo, L Viña-Soria, L Martín, M R Rodicio, D Escudero, F Vazquez, J N Mandrekar, R Patel
Carbapenemase-producing Enterobacteriaceae are increasing worldwide. Rectal screening for these bacteria can inform the management of infected and colonized patients, especially those admitted to intensive care units (ICUs). A laboratory developed, qualitative duplex real-time polymerase chain reaction assay for rapid detection of OXA-48-like and VIM producing Enterobacteriaceae, performed on rectal swabs, was designed and evaluated in an intensive care unit with endemic presence of OXA-48. During analytical assay validation, no cross-reactivity was observed and 100% sensitivity and specificity were obtained for both blaOXA-48-like and blaVIM in all spiked clinical samples...
April 6, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28438568/a-novel-mutation-in-pmrb-mediates-colistin-resistance-during-therapy-of-acinetobacter-baumannii
#3
Elias Dahdouh, Rosa Gómez-Gil, Sonia Sanz, Bruno González-Zorn, Ziad Daoud, Jesús Mingorance, Monica Suárez
Acinetobacter baumannii is a highly versatile nosocomial pathogen. Multidrug resistance among A. baumannii isolates led to the use of colistin, subsequently giving rise to colistin-resistant strains. In this study, the genetic and phenotypic profiles of two colistin-resistant A. baumannii isolates were investigated. Two A. baumannii isolates were obtained from Patient 1 (C071 and C440) and three isolates were obtained from Patient 2 (C080, C314 and C428). Susceptibility profiles were determined by VITEK(®)2 and Etest...
April 21, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28437228/first-case-of-ndm-1-producing-klebsiella-pneumoniae-in-annaba-university-hospital-algeria
#4
Amel Abderrahim, Nassima Djahmi, Charlotte Pujol, Sabina Nedjai, Mohamed Cherif Bentakouk, Djamila Kirane-Gacemi, Mazouz Dekhil, Albert Sotto, Jean-Philippe Lavigne, Alix Pantel
AIM: The aim of this study was to characterize two carbapenem-resistant Klebsiella pneumoniae isolates recovered from urine samples in a patient hospitalized at Annaba University hospital (Algeria) in 2014. RESULTS: Two K. pneumoniae isolates were studied because they proved resistant to almost all antibiotics tested with a high level resistance to ertapenem (minimum inhibitory concentration = 32 mg/L). The results of modified Hodge test and combined disk test (ROSCO Diagnostica, Taastrup, Denmark) were positive...
February 22, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28425271/molecular-characteristics-and-antibiotic-resistance-of-acinetobacter-baumanniibeta-lactamase-producing-isolates-a-predominance-of-intrinsic-blaoxa-51-and-detection-of-tem-and-ctx-m-genes
#5
Amir Ibrahimagić, Farah Kamberović, Selma Uzunović, Branka Bedenić, Emina Idrizović
BACKGROUND/AIM: The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December 2009 and May 2010. MATERIALS AND METHODS: Susceptibility testing was performed by disk diffusion and broth microdilution methods. The modified Hodge and combined disk test with EDTA/phenylboronic acid was used to screen for carbapenemase production...
April 18, 2017: Turkish Journal of Medical Sciences
https://www.readbyqxmd.com/read/28411078/performance-of-the-xpert-%C3%A2-carba-r-v2-in-the-daily-workflow-of-a-hygiene-unit-in-a-country-with-a-low-prevalence-of-carbapenemase-producing-enterobacteriaceae
#6
Yannick Hoyos, Souad Ouzani, Laurent Dortet, Nicolas Fortineau, Thierry Naas
Early detection of patients colonised with carbapenemase-producing Enterobacteriaceae (CPE) is crucial for implementing proper infection control measures. Here we evaluated the biological performance of the Xpert(®) Carba-R v2 (Cepheid) in the daily workflow of a hygiene unit in a country with a low CPE prevalence. Patients repatriated from countries known for high CPE prevalence or contact patients of a known CPE carrier were targeted as being 'high-risk patients' for CPE carriage. Between September 2015 and March 2016, 241 'high-risk patients' for CPE carriage were screened using the Xpert(®) Carba-R v2 and by plating on chromID(®) CARBA Smart medium (bioMérieux) with and without ertapenem-containing enrichment culture for 24 h...
April 12, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28389093/risk-factors-for-kpc-producing-enterobacteriaceae-acquisition-and-infection-in-a-healthcare-setting-with-possible-local-transmission-a-case-control-study
#7
K M Cronin, Y S Poy Lorenzo, M E Olenski, A E Bloch, K Visvanathan, M J Waters, K L Buising
BACKGROUND: Reports of Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae (KPC-Kp) in Australia were previously uncommon, with cases imported sporadically by travellers from higher prevalence countries. AIM: The study institution reported the first outbreak of KPC-Kp in Australia. The aim of this study was to identify risk factors for KPC-Kp colonization and infection using a matched case-control study. METHODS: The study included all hospitalized patients with KPC-Kp colonization or infection from January 2012 to September 2015...
February 16, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28380016/carbapenemase-producing-enterobacteriaceae-recovered-from-a-spanish-river-ecosystem
#8
Núria Piedra-Carrasco, Anna Fàbrega, William Calero-Cáceres, Thais Cornejo-Sánchez, Maryury Brown-Jaque, Alba Mir-Cros, Maite Muniesa, Juan José González-López
The increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. The dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including strains isolated from the environment. The objective of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) collected from a river ecosystem in the Barcelona metropolitan area (Spain). Identification of β-lactamases and other resistance determinants was determined as was the antimicrobial susceptibility profile...
2017: PloS One
https://www.readbyqxmd.com/read/28356417/use-of-ancillary-carbapenemase-tests-to-improve-specificity-of-phenotypic-definitions-for-carbapenemase-producing-enterobacteriaceae
#9
Shelley A Miller, Janet A Hindler, Angelo Chengcuenca, Romney M Humphries
Carbapenemase producing Enterobacteriaceae (CPE) are a significant threat to public health. In 2015, CDC revised the surveillance definition for CPE to include all Enterobacteriaceae resistant to any carbapenem tested. However, this definition is associated with poor specificity. We evaluated the performance of this definition for a collection of 125 Enterobacteriaceae, as compared to carbapenemase PCR. We also investigated the impact of ancillary testing for carbapenemase of isolates that met the CDC CPE surveillance definition...
March 29, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28351808/troubled-water-under-the-bridge-screening-of-river-mur-water-reveals-dominance-of-ctx-m-harboring-escherichia-coli-and-for-the-first-time-an-environmental-vim-1-producer-in-austria
#10
Gernot Zarfel, Michaela Lipp, Elena Gürtl, Bettina Folli, Rita Baumert, Clemens Kittinger
Antibiotic resistant bacteria (ARB) in the aquatic environment are reported from all over the world and their presence in the environment has become quite common. The current most prominent example is the presence of beta-lactamases harboring Enterobacteriaceae. The aim of this study was to investigate the presence and diversity (on the genetic and phenotypic levels) of extended spectrum beta-lactamases (ESBL) and carbapenemases harboring Enterobacteriaceae from the River Mur in the center of Graz, Austria's second largest city...
March 25, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28348155/molecular-%C3%AE-lactamase-characterization-of-aerobic-gram-negative-pathogens-recovered-from-patients-enrolled-in-the-ceftazidime-avibactam-phase-3-trials-for-complicated-intra-abdominal-infections-efficacies-analyzed-against-susceptible-and-resistant-subsets
#11
Rodrigo E Mendes, Mariana Castanheira, Leah N Woosley, Gregory G Stone, Patricia A Bradford, Robert K Flamm
The correlation of clinical efficacy of ceftazidime-avibactam (plus metronidazole) and meropenem was evaluated in subjects infected with Gram-negative isolates having characterized β-lactam resistance mechanisms from the complicated intra-abdominal infection (cIAI) phase 3 clinical trials. Enterobacteriaceae displaying ceftriaxone and/or ceftazidime MIC values of ≥2 μg/ml and Pseudomonas aeruginosa with ceftazidime MIC values of ≥16 μg/ml were characterized for extended-spectrum β-lactamase (ESBL) content...
March 27, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28337042/household-transmission-of-ndm-producing-e-coli-in-new-zealand
#12
Matthew Blakiston, Sally A Roberts, Joshua T Freeman, Helen Heffernan
This report describes the introduction of an extensively antibiotic-resistant carbapenemase-producing Escherichia coli into a hospital in Auckland, New Zealand, by a patient who was a household contact of recent travellers to the Indian subcontinent. The carbapenemase was identified as New Delhi metallo-β-lactamase (NDM) and reflects probable household transmission in the context of a recent upsurge in NDM-producing Enterobacteriaceae isolation in New Zealand. The observations in this report suggest that hospital screening practices to identify carbapenemase-producing Enterobacteriaceae (CPE) colonised patients may need to be extended to include travellers to high-risk countries who were not hospitalised during their trip, and possibly also their close contacts...
March 24, 2017: New Zealand Medical Journal
https://www.readbyqxmd.com/read/28320396/occurrence-of-bla-dha-1-mediated-cephalosporin-resistance-in-escherichia-coli-and-their-transcriptional-response-against-cephalosporin-stress-a-report-from-india
#13
Birson Ingti, Deepjyoti Paul, Anand Prakash Maurya, Debajyoti Bora, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee
BACKGROUND: Treatment alternatives for DHA-1 harboring strains are challenging as it confers resistance to broad spectrum cephalosporins and may further limit treatment option when expressed at higher levels. Therefore, this study was designed to know the prevalence of DHA genes and analyse the transcription level of DHA-1 against different β-lactam stress. METHODS: Screening of AmpC β-lactamase phenotypically by modified three dimensional extract method followed by Antimicrobial Susceptibility and MIC determination...
March 21, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28303831/strengths-and-limitations-of-various-screening-methods-for-carbapenem-resistant-enterobacteriaceae-including-new-method-recommended-by-clinical-and-laboratory-standards-institute-2017-a-tertiary-care-experience
#14
Agila Kumari Pragasam, Balaji Veeraraghavan, Yamuna Devi Bakthavatchalam, Radha Gopi, Raziya Fathima Aslam
Carbapenemase-mediated carbapenem resistance is a major concern across the world. Rapid detection of carbapenemase-producing organisms is of great importance in clinical settings. However, it is essential to have a test with good sensitivity and specificity. The aim of the study was to compare the performance of RAPIDEC® CARBA NP and modified carbapenem inactivation method (mCIM) recommended by Clinical and Laboratory Standards Institute guideline 2017. A total of ninety carbapenem resistant Escherichia coli and Klebsiella pneumoniae have been tested...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28298454/in-vitro-activity-of-imipenem-against-carbapenemase-positive-enterobacteriaceae-smart-global-surveillance-program-2008-2014
#15
James A Karlowsky, Sibylle H Lob, Krystyna M Kazmierczak, Robert E Badal, Katherine Young, Mary R Motyl, Daniel F Sahm
The Study for Monitoring Antimicrobial Resistance Trends (SMART) global surveillance program collected 103,960 isolates of Enterobacteriaceae from 2008 to 2014. From this isolate collection all ertapenem-non-susceptible isolates (MIC, ≥1 μg/ml; n = 3,428) and 9,371 isolates of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (ESBL)-positive phenotype were assessed for the presence of common carbapenemase genes using the Check-MDR CT101 microarray (Check-Points, Wageningen, the Netherlands) and published multiplex PCR assays...
March 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28291671/molecular-characterization-of-kpc-producing-klebsiella-pneumoniae-isolated-from-patients-in-a-public-hospital-in-caracas-venezuela
#16
Aura Dayana Falco Restrepo, Mariel Alexandra Velásquez Nieves, Howard Takiff
INTRODUCTION: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are amongst the most important causative agents of nosocomial infections worldwide. Isolates of this bacterium have been identified in Venezuela but little is known about their local spread. The aim of this study was to perform the molecular characterization of KPC-producing strains isolated from 2012 to 2013 in a public hospital in Caracas, Venezuela. METHODS: Twenty-two K. pneumoniae clinical isolates phenotypically classified as KPC producing were subjected to PCR screening for the presence of blaKPC genes and their location within transposon Tn4401...
March 11, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28288398/characterization-of-extended-spectrum-%C3%AE-lactamases-esbls-producing-salmonella-in-retail-raw-chicken-carcasses
#17
Jing Qiao, Qiang Zhang, Walid Q Alali, Jiawei Wang, Lingyuan Meng, Yingping Xiao, Hua Yang, Sheng Chen, Shenghui Cui, Baowei Yang
Extended-spectrum β-lactamases (ESBLs)-producing Salmonella is considered a serious concern to public health worldwide. However, limited information is available on ESBLs-producing Salmonella in retail chicken products in China. The objective of this study was to characterize ESBLs-producing Salmonella isolates from retail chickens in China. A total of 890 Salmonella isolates from retail chicken carcasses collected from 4 provinces were firstly screened for ESBLs-production phenotype via the double-disk synergy test method...
May 2, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28286140/detection-of-carbapenemase-producers-evaluating-the-performance-of-the-carbapenem-inactivation-method-and-carba-np-test-versus-multiplex-pcr
#18
Lamiaa A Madkour, May S Soliman, Dina M Hassan, Noha S Soliman, Yasmin A ElMahdy
OBJECTIVES: With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance of the carbapenem inactivation method (CIM) and the Carba NP test in the detection of carbapenemase-producers. METHODS: A panel of 203 Gram-negative bacterial isolates screened for carbapenem resistance were subjected to the CIM and Carba NP test...
March 9, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28254249/comparative-evaluation-of-a-new-commercial-media-the-chromagar%C3%A2-msupercarba%C3%A2-for-the-detection-of-carbapenemase-producing-enterobacteriaceae
#19
Ma'ayan Amar, Ohad Shalom, Amos Adler
A new chromogenic-based medium (mSuperCARBA™) was tested for screening carbapenemase-producing Enterobacteriaceae (CPE). mSuperCARBA™ was more sensitive (83%) in detecting CPE isolates (n=69, including KPC, NDM, OXA-48, VIM, and IMI) compared with CHROMAgar™-KPC (65%) and MacConkey agar with Imipenem (69%) with comparable specificity for non carbapenemase-producing, carbapenem-resistant Enterobacteriaceae (n=29).
May 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28251105/carbapenemase-producing-enterobacteriaceae-risk-factors-for-infection-and-impact-of-resistance-on-outcomes
#20
Shanthi Mariappan, Uma Sekar, Arunagiri Kamalanathan
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) have increased in recent years leading to limitations of treatment options. The present study was undertaken to detect CPE, risk factors for acquiring them and their impact on clinical outcomes. METHODS: This retrospective observational study included 111 clinically significant Enterobacteriaceae resistant to cephalosporins subclass III and exhibiting a positive modified Hodge test. Screening for carbapenemase production was done by phenotypic methods, and polymerase chain reaction was performed to detect genes encoding them...
January 2017: International Journal of Applied and Basic Medical Research
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