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Carbapenemase screening

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https://www.readbyqxmd.com/read/28799835/occurrence-of-carbapenemase-producing-enterobacteriaceae-isolates-in-the-wildlife-first-report-of-oxa-48-in-wild-boars-in-algeria
#1
Taous Bachiri, Sofiane Bakour, Rym Lalaoui, Nadia Belkebla, Meriem Allouache, Jean Marc Rolain, Abdelaziz Touati
The aim of the present study was to screen for the presence of carbapenemase-producing Enterobacteriaceae (CPE) isolates from wild boars and Barbary macaques in Algeria. Fecal samples were collected from wild boars (n = 168) and Barbary macaques (n = 212), in Bejaia, Algeria, between September 2014 and April 2016. The isolates were identified and antimicrobial susceptibility was determined. Carbapenem resistance determinants were studied using PCR and sequencing, while clonal relatedness was performed using multilocus sequence typing (MLST)...
August 11, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28795847/successful-containment-of-the-first-kpc-producing-klebsiella-pneumoniae-outbreak-in-croatia
#2
Dijana Varda Brkic, Irina Pristas, Ivan Cipris, Marko Jelic, Iva Butic, Arjana Tambic Andrasevic
AIM: We report the first outbreak caused by Klebsiella pneumoniae carbapenemase (KPC)-producing K. pneumoniae sequence type 258 (ST 258) clone that occurred in Croatia from May to December 2012. MATERIALS & METHODS: 23 carbapenem-resistant K. pneumoniae isolates were recovered from clinical and screening specimens of 12 patients hospitalized in a regional hospital. The first isolates from the 12 patients were typed using pulsed-field gel electrophoresis and multilocus sequence typing...
August 10, 2017: Future Microbiology
https://www.readbyqxmd.com/read/28792697/the-first-enterobacter-cloacae-co-producing-ndm-and-oxa-48-carbapenemases-and-interhospital-spread-of-oxa-48-and-ndm-producing-klebsiella-pneumoniae-in-turkey
#3
Demet Haciseyitoglu, Aysegul Dokutan, Ayham Abulaila, Fatma Erdem, Yasemin Cag, Serdar Ozer, Zerrin Aktas
BACKGROUND: The aim of this study was to investigate the occurrence of carbapenemase-producing Enterobacteriaceae. METHODS: A total of 54 carbapenem nonsusceptible Enterobacteriaceae (CRE) isolates were recovered from clinical samples sent to the Dr. Lutfi Kirdar Kartal Training and Research Hospital from the period 2011 through 2014. Forty-four isolates were Klebsiella pneumoniae (CRKP) and the other 10 were Enterobacter cloacae (CREC).The isolate identifications and antibiotic sensitivity tests were performed using a Vitek2 automatic system...
July 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28760848/comprehensive-arrayed-transposon-mutant-library-of-klebsiella-pneumoniae-outbreak-strain-kpnih1
#4
Beth Ramage, Roxanne Erolin, Kiara Held, Joe Gasper, Eli Weiss, Mitch Brittnacher, Larry Gallagher, Colin Manoil
Klebsiella pneumoniae and other carbapenem-resistant Enterobacteriaceae are a major cause of hospital-acquired infections, yet the basis of their success as nosocomial pathogens is poorly understood. To help provide a foundation for genetic analysis of K. pneumoniae, we created an arrayed, sequence-defined transposon mutant library of an isolate from the 2011 outbreak of infections at the U.S. National Institutes of Health Clinical Center. The library is made up of 12,000 individually arrayed mutants of a carbapenemase deletion parent strain and provides coverage of 85% of predicted genes...
July 31, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28743652/molecular-epidemiology-of-carbapenem-resistant-klebsiella-pneumonia-at-a-turkish-center-is-the-increase-of-resistance-a-threat-for-europe
#5
Aslıhan Candevir Ulu, Tülin Güven Gökmen, Filiz Kibar, Behice Kurtaran, Cansu Önlen, Ferit Kuşçu, Ayşe Seza İnal, Süheyla Kömür, Akgün Yaman, Hasan Salih Zeki Aksu, Yeşim Taşova
INTRODUCTION: In recent years carbapenem resistant Klebsiella pneumoniae has became an important threat to hospitalized patients. Our aim in this study was to identify the genetic mechanisms of carbapenem resistance in our center. MATERIALS AND METHODS: In this study, a total of 98K. pneumoniae isolated from patients who registered at Cukurova University Balcali Hospital and determined phenotypically carbapenem resistant were screened for genotypic presence of carbapenemase enzymes with the multiplex-PCR in the period of 2013-2014...
July 22, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28743649/first-report-of-blaoxa-181-mediated-carbapenem-resistance-in-aeromonas-caviae-in-association-with-pkp3-a-threat-for-rapid-dissemination
#6
Shalini Anandan, Radha Gopi, Naveen Kumar Devanga Ragupathi, Dhiviya Prabaa Muthuirulandi Sethuvel, Priya Gunasekaran, Kamini Walia, Balaji Veeraraghavan
OBJECTIVES: Aeromonas spp producing carbapenemases is of great concern in health-care settings and also known to acquire clinically relevant resistance genes. In this study, carbapenem non-susceptible Aeromonas isolates were characterized for their molecular mechanism. METHOD: Out of 180 Aeromonas isolates, 10 carbapenem non-susceptible isolates were selected based on their antimicrobial susceptibility profile. Carbapenemase production was investigated using CarbaNP method...
July 22, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28730149/review-of-antibiotic-resistance-in-the-indian-ocean-commission-a-human-and-animal-health-issue
#7
REVIEW
Noellie Gay, Olivier Belmonte, Jean-Marc Collard, Mohamed Halifa, Mohammad Iqbal Issack, Saindou Mindjae, Philippe Palmyre, Abdul Aziz Ibrahim, Harena Rasamoelina, Loïc Flachet, Laurent Filleul, Eric Cardinale
Antimicrobial resistance (AMR) is a major threat to human, animal health, and environment worldwide. For human, transmission occurred through a variety of routes both in health-care settings and community. In animals, AMR was reported in livestock, pets, and wildlife; transmission of AMR can be zoonotic with the probably most important route being foodborne transmission. The Indian Ocean Commission (IOC), composed of Comoros, Madagascar, Mauritius, Reunion (France), and Seychelles recognized the surveillance of AMR in both animal and human as a main public health priority for the region...
2017: Frontiers in Public Health
https://www.readbyqxmd.com/read/28721477/imipenem-resistant-gram-negative-bacterial-isolates-carried-by-persons-upon-medical-examination-in-korea
#8
So Yeon Kim, Sang Yop Shin, Ji-Young Rhee, Kwan Soo Ko
Carbapenem-resistant Gram-negative bacteria (CR-GNB) have emerged and disseminated worldwide, become a great concern worldwide including Korea. The prevalence of fecal carriage of imipenem-resistant Gram-negative bacteria (IR-GNB) in persons in Korea was investigated. Stool samples were collected from 300 persons upon medical examination. Samples were screened for IR-GNB by using MacConkey agar with 2 μl/ml imipenem. Species were identified by 16S rRNA gene sequence analysis, and antimicrobial susceptibility was determined by the broth microdilution method...
August 2017: Journal of Microbiology / the Microbiological Society of Korea
https://www.readbyqxmd.com/read/28711441/emergence-of-carbapenem-resistant-acinetobacter-baumannii-st787-in-clinical-isolates-from-blood-in-a-tertiary-teaching-hospital-in-northern-taiwan
#9
Yi-Fan Hu, Charles Jia-Yin Hou, Chien-Feng Kuo, Nai-Yu Wang, Alice Ying-Jung Wu, Ching-Hsiang Leung, Chang-Pan Liu, Hung-I Yeh
BACKGROUND/PURPOSE: The purpose of this study is to investigate the predominant clones of carbapenem-resistant Acinetobacter baumannii (CRAB) in our hospital in Taiwan by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) technique. METHODS: We collected 108 non-duplicate A. baumannii clinical blood isolates from January 2012 to December 2013 in MacKay Memorial Hospital. PFGE and MLST were used for typing the A. baumannii isolates and for investigation of the predominant clones...
June 22, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28711315/characterization-of-mobile-genetic-elements-carrying-vim-1-and-kpc-2-carbapenemases-in-citrobacter-freundii-isolates-in-madrid
#10
Jennifer Villa, David M Arana, Esther Viedma, Dafne Perez-Montarelo, Fernando Chaves
Carbapenemase producing Citrobacter freundii (CPCF) infections are still uncommon in European countries. Here we report a molecular study conducted in a tertiary care facility in southern Madrid, Spain, from 2009 to 2014 to investigate the epidemiology of CPCF. The blaIMP-1,blaIMP-2,blaKPC,blaNDM,blaOXA-48,blaVIM-1 and blaVIM-2 genes were screened by PCR. Molecular typing was carried out by Pulsed-field gel electrophoresis analysis (PFGE) and multilocus sequence typing (MLST). Whole genome sequencing (WGS) was performed to characterize the resistome and the mobile genetic elements associated with the carbapenems resistance of CPCF...
July 8, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28706391/utility-of-a-novel-chromogenic-medium-as-a-screening-method-in-the-detection-of-carbapenemase-producing-enterobacteriaceae
#11
Anusha Chaturvedi, Gunjiganur Shankarappa Banashankari
INTRODUCTION: With the emergence of multidrug resistant Gram-negative bugs, there is an urgent need for rapid detection of these resistant organisms. AIM: This study was performed to assess the utility of a novel chromogenic medium in the detection of carbapenemase producing Enterobacteriaceae from various clinical specimens. MATERIALS AND METHODS: A total of 202 isolates of Enterobacteriaceae, which were resistant to meropenem (10 μg) disc by standard disc diffusion method, were analyzed over a period of 6 months...
July 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28686503/characterization-of-carbapenem-resistant-escherichia-coli-isolates-through-the-whole-genome-sequencing-analysis
#12
Yanping Luo, Rong Luo, Hong Ding, Xiu Ren, Haipeng Luo, Ying Zhang, Liyan Ye, Shenghui Cui
Limited studies have characterized multiple carbapenem-resistant Escherichia coli isolates and compared their genetic characteristics through whole-genome sequencing (WGS) because of the scarcity of these isolates. In this study, we determined the prevalence of carbapenem resistance in 2,993 E. coli isolates collected from three Chinese hospitals. The carbapenem-resistant isolates were further characterized by WGS, and the molecular epidemiological characters and resistance mechanisms were explored through the publicly available platforms from the Center for Genomic Epidemiology...
July 7, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28685856/the-performance-of-a-resazurin-chromogenic-agar-plate-with-a-combined-disc-method-for-rapid-screening-of-extended-spectrum-%C3%AE-lactamases-ampc-%C3%AE-lactamases-and-co-%C3%AE-lactamases-in-enterobacteriaceae
#13
Yothin Teethaisong, Katie Evans, Ismini Nakouti, Kanokwan Tiamyom, James R Ketudat-Cairns, Glyn Hobbs, Griangsak Eumkeb
A resazurin chromogenic agar (RCA) along with combined disc method has been developed as a promising method for rapid screening of extended-spectrum-β-lactamase (ESBL), AmpC β-lactamase, and co-production of ESBL and AmpC. Cefpodoxime (CPD) discs supplemented with and without clavulanic acid (CA), cloxacillin (CX), or CA+CX were evaluated against 86-molecularly confirmed β-lactamase-producing Enterobacteriaceae, including 15 ESBLs, 32 AmpCs, 9 co-producers of ESBL and AmpC, and 30 carbapenemase producers...
July 7, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28671522/molecular-epidemiology-of-beta-lactamase-producing-nosocomial-gram-negative-pathogens-from-north-and-south-indian-hospitals
#14
Surbhi Khurana, Purva Mathur, Arti Kapil, Chithra Valsan, Bijayini Behera
PURPOSE: Resistant Gram-negative bacterial (GNB) infections, apart from tremendously escalating the cost of treatment, are a cause for substantial morbidity and mortality among hospitalized patients. Such bacteria are rapidly acquiring resistance to many antimicrobial agents, especially the beta-lactams which are the most frequently prescribed antimicrobials in hospital and community patient care settings, and now also to colistin; a last-line drug to treat infections with such bacteria...
July 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28670642/multilocus-sequence-typing-of-carbapenem-resistant-pseudomonas-aeruginosa-isolates-from-patients-presenting-at-port-elizabeth-hospitals-south-africa
#15
D Annear, J Black, S Govender
BACKGROUND: Pseudomonas aeruginosa is an important nosocomial pathogen that exhibits multiple drug resistance with increasing frequency, especially to carbapenems making patient treatment difficult. Carbapenem-resistance may be caused by porin gene mutations, active drug efflux, and carbapenemase production. This study evaluated the incidence of genes responsible for carbapenemase production in carbapenem-resistant Pseudomonas aeruginosa and assessed the genetic relatedness of the isolates by multi locus sequence typing (MLST)...
2017: African Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28668692/wastewater-is-a-reservoir-for-clinically-relevant-carbapenemase-and-16s-rrna-methylase-producing-enterobacteriaceae
#16
Katrin Zurfluh, Claudia Bagutti, Peter Brodmann, Monica Alt, Jürg Schulze, Séamus Fanning, Roger Stephan, Magdalena Nüesch-Inderbinen
The aim of the study was to evaluate wastewater for carbapenemase producing Enterobacteriaceae (CPE) and 16S rRNA methylase producing Gram negative bacteria (MPB), and to assess their occurrence following wastewater treatment. Wastewater samples were collected between June 2015 and March 2016 in the sewage network of the city of Basel from sites located before and after influx of wastewater from the hospital into the sewage network. Samples were also obtained from the influent and from the effluent of the receiving wastewater treatment plant...
June 28, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28666753/first-report-of-an-escherichia-coli-strain-from-swine-carrying-an-oxa-181-carbapenemase-and-the-colistin-resistance-determinant-mcr-1
#17
Sandra Pulss, Torsten Semmler, Ellen Prenger-Berninghoff, Rolf Bauerfeind, Christa Ewers
Plasmid-mediated resistance to carbapenems and colistin in Enterobacteriaceae represents an emerging public health threat. Although animals have been identified as a relevant source of multidrug-resistant (MDR) bacteria, there are only a few reports on the presence of carbapenemases in animal isolates. In this study, 7850 faecal Escherichia coli isolates obtained from 2160 pigs were screened for carbapenem non-susceptibility using Mueller-Hinton agar supplemented with meropenem. Eleven isolates showed growth on meropenem-containing agar but only two proved positive by PCR for a carbapenemase gene, namely blaOXA-48-like...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28666749/prevalences-of-antimicrobial-resistance-and-the-cfia-resistance-gene-in-danish-bacteroides-fragilis-group-isolates-since-1973
#18
Simon Andreas Ferløv-Schwensen, Thomas Vognbjerg Sydenham, Kia Cirkeline Hansen, Silje Vermedal Hoegh, Ulrik Stenz Justesen
OBJECTIVES: The purpose of this study was to determine the prevalences of resistance and the cfiA carbapenemase-producing gene in historical Bacteroides fragilis group isolates. METHODS: Danish clinical B. fragilis group isolates (n=444) spanning from 1973 to 2015 were identified with Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) on the Biotyper platform. Antimicrobial resistance was determined using a disk diffusion screening method and commercial antibiotic gradient strips...
June 27, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28652234/meropenem-vaborbactam-tested-against-contemporary-gram-negative-isolates-collected-worldwide-during-2014-including-carbapenem-resistant-kpc-producing-multidrug-resistant-and-extensively-drug-resistant-enterobacteriaceae
#19
Mariana Castanheira, Michael D Huband, Rodrigo E Mendes, Robert K Flamm
We evaluated the activity of meropenem-vaborbactam against contemporary non-fastidious gram-negative clinical isolates, including resistant phenotypes and carbapenemase genotypes of Enterobacteriaceae Meropenem-vaborbactam (inhibitor at 8 μg/ml) and comparators were susceptibility tested using reference broth microdilution methods against 14,304 gram-negative clinical isolates collected worldwide during 2014. Carbapenemase encoding genes were screened by PCR/sequencing. Meropenem-vaborbactam (MIC50/90, ≤0...
June 26, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28642140/emergence-of-carbapenem-resistant-enterobacteriaceae-isolated-from-patients-in-a-university-hospital-in-saudi-arabia-epidemiology-clinical-profiles-and-outcomes
#20
Fawzia E Alotaibi, Elham E Bukhari, Maha M Al-Mohizea, Taghreed Hafiz, Eman Bin Essa, Yasmeen I AlTokhais
Carbapenemase-producing Enterobacteriaceae have been steadily spreading worldwide during the last decade. Nine patients were identified prospectively and were followed during their hospitalization course to identify the epidemiology, clinical profiles and outcomes. These patients had one or more cultures positive for a CRE isolate, contributing to a total of eleven positive cultures from various sites without including duplicates of isolates obtained from the same site. Isolates from these patients included five Klebseilla pneumoniae, three Escherichia coli, and one Enterobacter aerogenes...
June 19, 2017: Journal of Infection and Public Health
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