keyword
https://read.qxmd.com/read/38171475/induction-of-human-esc-derived-and-adult-primary-multipotent-limbal-stem-cells-into-retinal-pigment-epithelial-cells-and-corneal-stromal-stem-cells
#21
JOURNAL ARTICLE
Juan Yang, Meiyu Tian, Jinyang Li, Yu Chen, Shichao Lin, Xiaoyin Ma, Wei Chen, Ling Hou
Human embryonic stem cell (hESC)- and human induced pluripotent stem cell (hiPSC)-derived retinal pigment epithelium (RPE) therapies are promising alternatives for the treatment of retinal degenerative diseases caused by RPE degeneration. The generation of autologous RPE cells from human adult donors, which has the advantage of avoiding immune rejection and teratoma formation, is an alternative cell resource to gain mechanistic insight into and test potential therapies for RPE degenerative diseases. Here, we found that limbal stem cells (LSCs) from hESCs and adult primary human limbus have the potential to produce RPE cells and corneal stromal stem cells (CSSCs)...
January 1, 2024: Experimental Eye Research
https://read.qxmd.com/read/38168310/gsk3-inhibition-reduces-ecm-production-and-prevents-age-related-macular-degeneration-like-pathology
#22
Sophia M DiCesare, Antonio J Ortega, Gracen E Collier, Steffi Daniel, Krista N Thompson, Melissa K McCoy, Bruce A Posner, John D Hulleman
Malattia Leventinese/Doyne Honeycomb Retinal Dystrophy (ML/DHRD) is an age-related macular degeneration (AMD)-like retinal dystrophy caused by an autosomal dominant R345W mutation in the secreted glycoprotein, fibulin-3 (F3). To identify new small molecules that reduce F3 production from retinal pigmented epithelium (RPE) cells, we knocked-in a luminescent peptide tag (HiBiT) into the endogenous F3 locus which enabled simple, sensitive, and high throughput detection of the protein. The GSK3 inhibitor, CHIR99021 (CHIR), significantly reduced F3 burden (expression, secretion, and intracellular levels) in immortalized RPE and non-RPE cells...
December 15, 2023: bioRxiv
https://read.qxmd.com/read/38159411/the-role-of-retinal-glial-cells-and-related-factors-in-macular-edema
#23
REVIEW
Qi Zhang, Shounan Qi, Jiaxin You, Chenguang Wang
Macular edema (ME) has emerged as a leading cause of visual impairment, representing a critical clinical manifestation and complication associated with many eye diseases. In the occurrence and development of ME, retinal glial cells like Müller cells and microglial cells play vital roles. Moreover, growth factor and cytokines associated with them, such as vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF), hypoxia-inducible factor-1α (HIF-1α), angiopoietin-like protein 4 (ANGPTL4), interleukin-6(IL-6), interleukin-8 (IL-8), monocyte chemoattractant protein-1 (MCP-1), prostaglandin, etc...
December 23, 2023: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38153666/pedf-protects-retinal-pigment-epithelium-from-ferroptosis-and-ameliorates-dry-amd-like-pathology-in-a-murine-model
#24
JOURNAL ARTICLE
Wei Xiang, Longhui Li, Qin Zhao, Yongcheng Zeng, Jinhui Shi, Zitong Chen, Guoquan Gao, Kunbei Lai
Age-related macular degeneration (AMD) is the leading cause of irreversible vision damage among elderly individuals. There is still no efficient treatment for dry AMD. Retinal pigment epithelial (RPE) degeneration has been confirmed to play an important role in dry AMD. Recent studies have reported that ferroptosis caused by iron overload and lipid peroxidation may be the primary causes of RPE degeneration. However, the upstream regulatory molecules of RPE ferroptosis remain largely unknown. Pigment epithelium-derived factor (PEDF) is an important endogenic protective factor for the RPE...
December 28, 2023: GeroScience
https://read.qxmd.com/read/38141777/12-hete-activates-m%C3%A3-ller-glial-cells-the-potential-role-of-gpr31-and-mir-29
#25
REVIEW
Mohamed Moustafa, Abraham Khalil, Noureldien H E Darwish, Dao-Qi Zhang, Amany Tawfik, Mohamed Al-Shabrawey
Diabetic retinopathy (DR) is a neurovascular complication of diabetes, driven by an intricate network of cellular and molecular mechanisms. This study sought to explore the mechanisms by investigating the role of 12-hydroxyeicosatetraenoic acid (12-HETE), its receptor GPR31, and microRNA (miR-29) in the context of DR, specifically focusing on their impact on Müller glial cells. We found that 12-HETE activates Müller cells (MCs), elevates glutamate production, and induces inflammatory and oxidative responses, all of which are instrumental in DR progression...
December 21, 2023: Prostaglandins & Other Lipid Mediators
https://read.qxmd.com/read/38137423/protective-effect-of-topiramate-against-diabetic-retinopathy-and-computational-approach-recognizing-the-role-of-nlrp3-il-1%C3%AE-tnf-%C3%AE-signaling
#26
JOURNAL ARTICLE
Hala M F Mohammad, Mohamed Ahmed Eladl, Asmaa K K Abdelmaogood, Rabie E Elshaer, Walaa Ghanam, Abdelhakeem Elaskary, Mohamed A K Saleh, Amira H Eltrawy, Sahar K Ali, Suzan M M Moursi, Shymaa E Bilasy, Sawsan A Zaitone, Wafa Ali Alzlaiq, Hayam Atteya
The possible impact of topiramate against diabetic retinopathy (DREN) and its molecular mechanisms in relation to the nod-like receptor family pyrin domain containing 3 (NLRP3) inflammasome has not been studied before. Thus, in the present study, we aimed to utilize a computational approach to investigate the possible protective effect of topiramate on experimental DREN and explore its impact on NLRP3/interlukin-1β signaling and brain-derived neurotrophic factor (BDNF) expression. Male albino mice were distributed to four experimental groups and assigned the following categorizations: (i) saline, (ii) diabetic, (iii) diabetic + topiramate 10 mg/kg and (iv) diabetic + topiramate 30 mg/kg...
December 1, 2023: Biomedicines
https://read.qxmd.com/read/38098048/comparison-of-retinal-degeneration-treatment-with-four-types-of-different-mesenchymal-stem-cells-human-induced-pluripotent-stem-cells-and-rpe-cells-in-a-rat-retinal-degeneration-model
#27
JOURNAL ARTICLE
Qian Liu, Jun Liu, Minmei Guo, Tzu-Cheng Sung, Ting Wang, Tao Yu, Zeyu Tian, Guoping Fan, Wencan Wu, Akon Higuchi
BACKGROUND: Retinal degeneration (RD) is a group of disorders on irreversible vision loss. Multiple types of stem cells were used in clinical trials for RD treatment. However, it remains unknown what kinds of stem cells are most effective for the treatment. Therefore, we investigated the subretinal transplantation of several types of stem cells, human adipose-derived stem cells (hADSCs), amniotic fluid stem cells (hAFSCs), bone marrow stem cells (hBMSCs), dental pulp stem cells (hDPSCs), induced pluripotent stem cell (hiPSC), and hiPSC-derived retinal pigment epithelium (RPE) cells for protection effects, paracrine effects and treatment efficiency in an RD disease model rats...
December 14, 2023: Journal of Translational Medicine
https://read.qxmd.com/read/38084239/the-effect-of-calcium-dobesilate-combined-with-hypoglycemic-drugs-in-the-treatment-of-cataract-npdr-and-its-effect-on-fundus-microcirculation-and-blood-icam-1-mcp-1-and-mif-levels
#28
JOURNAL ARTICLE
XiaoMan Wang, Zhao Zhang, Yi Qing Ge, Xia Wu, Yin Chao Ma
BACKGROUND: To explore the effect of calcium dobesilate combined with hypoglycemic drugs in the treatment of cataract complicated with non-proliferative diabetic retinopathy (NPDR) and its effects on fundus microcirculation, intercellular adhesion molecule 1 (ICAM-1), mono - cyte chemoattractant protein 1 (MCP-1), and macrophage migration inhibitory factor (MIF). METHODS: From March 2019 to January 2021, a total of 114 patients with cataract and NPDR were included, and the patients were assigned into the control and the observation groups by random number table method, with 57 cases/group...
October 27, 2023: Journal of Medical Biochemistry
https://read.qxmd.com/read/38069124/alteration-in-melanin-content-in-retinal-pigment-epithelial-cells-upon-hydroquinone-exposure
#29
JOURNAL ARTICLE
Takeyuki Nishiyama, Hiroki Tsujinaka, Tetsuo Ueda, Nahoko Ogata
Abnormal pigmentation or depigmentation of the retinal pigment epithelium (RPE) is a precursor to neovascular age-related macular degeneration (nAMD). In this study, we evaluated the effects of hydroquinone (HQ), the most potent reductant in cigarette smoke, on the melanin production in RPE cells. Induced pluripotent stem cell (iPS)-derived RPE and adult retinal pigment epithelial (ARPE-19) cells were cultured with HQ. Real-time reverse transcription polymerase chain reaction revealed that the expression of melanin-related genes decreased due to the addition of HQ for 1 day...
November 27, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38062839/pedf-prevents-mitochondrial-function-decay-and-er-stress-induced-by-rotenone-in-aging-rpe-cells
#30
JOURNAL ARTICLE
Ruixue Zhang, Zhi Ji, Zhuoya Quan, Yao Lu, Yuan Ren, Yuan He
BACKGROUND: Neurodegenerative diseases, including age-related macular degeneration (AMD), may be linked to mitochondrial dysfunction and endoplasmic reticulum (ER) stress. We examined whether Pigment epithelium-derived factor (PEDF) could prevent changes in the structure and function of these organelles by accelerating by rotenone (ROT), a mitochondrial inhibitor, in human retinal pigment epithelium (RPE) cells of chronological age. METHODS: RPE cells from 9-20, 50-55, 60-70, and >70-year-old donors were isolated, grown as primary cultures, harvested, and treated with ROT and PEDF for electron microscope (EM), western blot analysis, and polymerase chain reaction (PCR)...
November 29, 2023: Frontiers in Bioscience (Landmark Edition)
https://read.qxmd.com/read/38056550/short-peptides-derived-from-pigment-epithelium-derived-factor-attenuate-retinal-ischemia-reperfusion-injury-through-inhibition-of-apoptosis-and-inflammatory-response-in-rats
#31
JOURNAL ARTICLE
Ming-Hui Sun, Tsung-Chuan Ho, Shu-I Yeh, Show-Li Chen, Yeou-Ping Tsao
Pigment epithelium-derived factor (PEDF) is widely recognized as a neuroprotective factor expressed in the retina and has shown therapeutic potential in several retinal diseases. Our study aimed to identify the neuroprotective fragment in PEDF and investigate its protective activity in retinas under ischemia-reperfusion (IR) condition. We synthesized a series of shorter synthetic peptides, 6-mer (Ser93-Gln98) and its d-form variant (6 dS) derived from the 44-mer (Val78-Thr121; a PEDF neurotrophic fragment), to determine their cytoprotective activity in IR injury, which was induced in rat retinas by injection of saline into the anterior chamber to increase the intraocular pressure (IOP) followed by reperfusion...
December 4, 2023: Experimental Eye Research
https://read.qxmd.com/read/38055741/pathologic-vs-protective-roles-of-hypoxia-inducible-factor-1-in-rpe-and-photoreceptors-in-wet-vs-dry-age-related-macular-degeneration
#32
JOURNAL ARTICLE
Savalan Babapoor-Farrokhran, Yu Qin, Miguel Flores-Bellver, Yueqi Niu, Imran A Bhutto, Silvia Aparicio-Domingo, Chuanyu Guo, Murilo Rodrigues, Timothy Domashevich, Monika Deshpande, Haley Megarity, Rakesh Chopde, Charles G Eberhart, Valeria Canto-Soler, Silvia Montaner, Akrit Sodhi
It has previously been reported that antioxidant vitamins can help reduce the risk of vision loss associated with progression to advanced age-related macular degeneration (AMD), a leading cause of visual impairment among the elderly. Nonetheless, how oxidative stress contributes to the development of choroidal neovascularization (CNV) in some AMD patients and geographic atrophy (GA) in others is poorly understood. Here, we provide evidence demonstrating that oxidative stress cooperates with hypoxia to synergistically stimulate the accumulation of hypoxia-inducible factor (HIF)-1α in the retinal pigment epithelium (RPE), resulting in increased expression of the HIF-1-dependent angiogenic mediators that promote CNV...
December 12, 2023: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/38052851/pigment-epithelium-derived-factor-pedf-represses-the-glucose-transporter-1-glut1-mrna-expression-and-may-be-a-potential-therapeutic-agent-in-psoriasis-a-case-control-and-experimental-study
#33
JOURNAL ARTICLE
Khalid M Mohany, Sherouk Elkady, Eman M Kamal Youssef, Noorhan M Sayed, Naglaa Kamal Idriss
We investigated the whole blood GLUT1 mRNA expression and serum pigment epithelium-derived factor (PEDF), interleukin-6 (IL-6), fetuin-A, and pentraxin-3 (PTX3) levels in psoriatic patients and tested their correlations with the severity of psoriasis using the psoriasis area and severity index (PASI) score. Also, we tested the GLUT1 mRNA expression after an in vitro treatment of human skin fibroblast (HSF) cell lines with PEDF. The case-control part of the study recruited 74 participants (44 psoriatic patients and 30 healthy volunteers)...
December 5, 2023: Scientific Reports
https://read.qxmd.com/read/38047739/correlation-between-vitreous-level-of-angiogenic-growth-factors-and-oxygen-saturation-in-retinal-vessels-in-diabetic-retinopathy
#34
JOURNAL ARTICLE
Hana Morin, Jan Havlík, Oldrich Chrapek, Michal Hrevuš, Pavel Nemec, Leoš Rejmont, Jan Tesar, Marta Kalousova, Tomaš Zima, Martin Šín
PURPOSE: The aim of this study was to investigate whether there is any association between the levels of the angiogenic growth factors and the vascular oxygen saturation in eyes with diabetic retinopathy. METHODS: The study was designed as a prospective trial. The cohort consisted of 29 diabetic patients with scheduled vitreous procedures (intravitreal injection or pars plana vitrectomy). The control group included 30 patients scheduled for macular surgery (macular hole or epiretinal membrane)...
December 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/38036037/a-new-r-r-rvd6-isomer-with-protective-actions-following-corneal-nerve-injury
#35
REVIEW
Haydee E P Bazan, Thang L Pham
The transparent cornea is the most densely innervated tissue in the body, primarily by sensory nerves originating from the trigeminal ganglia (TG). Damage to corneal nerves reduces sensitivity and tear secretion and results in dry eye. Consequently, ocular pain, for which no satisfactory therapies exist, arises in many cases. Treatment of injured corneas with pigment epithelium-derived factor (PEDF) combined with docosahexaenoic acid (DHA) stimulates nerve regeneration in models of refractive surgery, which damages nerves...
November 28, 2023: Prostaglandins & Other Lipid Mediators
https://read.qxmd.com/read/37922158/medium-depth-influences-o2-availability-and-metabolism-in-human-rpe-cultures
#36
JOURNAL ARTICLE
Daniel T Hass, Qitao Zhang, Gillian A Autterson, Richard A Bryan, James B Hurley, Jason M L Miller
PURPOSE: Retinal pigment epithelium (RPE) oxidative metabolism is critical for normal retinal function and is often studied in cell culture systems. Here, we show that conventional culture media volumes dramatically impact O2 availability, limiting oxidative metabolism. We suggest optimal conditions to ensure cultured RPE is in a normoxic environment permissive to oxidative metabolism. METHODS: We altered the availability of O2 to human primary and induced pluripotent stem cell-derived RPE cultures directly via a hypoxia chamber or indirectly via the amount of medium over cells...
November 1, 2023: Investigative Ophthalmology & Visual Science
https://read.qxmd.com/read/37918568/balance-of-gata3-and-ramp2-in-hepatocytes-regulates-hepatic-vascular-reconstitution-in-postoperative-liver-regeneration
#37
JOURNAL ARTICLE
Bibo Wang, Hao Shen, Yating Wei, Fuchen Liu, Yuan Yang, Han Yu, Jing Fu, Xiuliang Cui, Ting Yu, Ying Xu, Yitian Liu, Hui Dong, Feng Shen, Weiping Zhou, Hui Liu, Yao Chen, Hongyang Wang
BACKGROUND & AIMS: Post-hepatectomy liver failure (PHLF) leads to poor prognosis in patients undergoing hepatectomy, with hepatic vascular reconstitution playing a critical role. However, the regulators of hepatic vascular reconstitution remain unclear. This study aimed to investigate the regulatory mechanisms of hepatic vascular reconstitution and identify biomarkers predicting PHLF in patients undergoing hepatectomy. METHODS: Candidate genes that were associated with hepatic vascular reconstitution were screened using adeno-associated virus vectors in Alb-Cre-CRISPR/Cas9 mice subjected to partial hepatectomy...
October 31, 2023: Journal of Hepatology
https://read.qxmd.com/read/37871271/inflammatory-cell-activity-in-treated-neovascular-age-related-macular-degeneration-a-histologic-case-study
#38
JOURNAL ARTICLE
Andreas Berlin, Jeffrey D Messinger, Prithvi Ramtohul, Chandrakumar Balaratnasingam, Randev Mendis, Daniela Ferrara, K Bailey Freund, Christine A Curcio
BACKGROUND: Imaging indicators of macular neovascularization risk can help determine patient eligibility for new treatments for geographic atrophy secondary to age-related macular degeneration. Because type 1 macular neovascularization includes inflammation, we assessed by histology the distribution of cells with inflammatory potential in two fellow eyes with age-related macular degeneration. METHODS: Two eyes of a White woman in her 90's with type 3 macular neovascularization treated with antivascular endothelial growth factor were prepared for high-resolution histology...
November 1, 2023: Retina
https://read.qxmd.com/read/37839784/characterization-of-three-adults-and-an-adolescent-with-osteogenesis-imperfecta-type-vi-and-a-novel-founder-serpinf1-variant
#39
JOURNAL ARTICLE
André M Travessa, Patrícia Dias, Joana Rosmaninho-Salgado, Miriam Aza-Carmona, Oana Moldovan, Francisca Díaz-González, Fátima Godinho, José Carlos Romeu, Filipa Oliveira-Ramos, Maria do Céu Barreiros, Sérgio B Sousa, Karen E Heath, Ana Berta Sousa
Osteogenesis imperfecta (OI) type VI is an extremely rare form of OI caused by biallelic variants in the SERPINF1 gene, which codes for the pigment-epithelium derived factor (PEDF). We report on four patients (three adults and one adolescent) with a severe deforming form of OI. All patients presented no abnormalities at birth, frequent long bone and vertebrae fractures (mainly during childhood), marked short stature, severe bone deformities, chronic mild to moderate pain, and severe limitation of mobility, with three being completely wheelchair bound...
October 13, 2023: European Journal of Medical Genetics
https://read.qxmd.com/read/37820680/effectiveness-differences-of-ranibizumab-and-aflibercept-action-in-the-treatment-of-diabetic-retinopathy
#40
JOURNAL ARTICLE
Xiaoli Ma, Nannan Ma, Qianqian Zhang, Yonghong Wang, Haixia Sun, Zhenyuan Yang
OBJECTIVE: To compare the difference in the effectiveness of ranibizumab (LU) and aflibercept (AF) in the treatment of diabetic retinopathy (DR). METHODS: Ninety-four patients with DR admitted to Sunshine Union Hospital from August 2020 to February 2022 were selected for the study and were divided into LU group (n = 47) and AF group (n = 47) according to the random number table method. Both groups underwent 25G vitrectomy in our hospital, with LU injected into the vitreous before surgery in the LU group and AF in the AF group...
October 13, 2023: Alternative Therapies in Health and Medicine
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