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Pigment epithelium derived factor

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https://www.readbyqxmd.com/read/28730105/pigment-epithelium-derived-factor-protects-retinal-ganglion-cells-from-hypoxia-induced-apoptosis-by-preventing-mitochondrial-dysfunction
#1
Shu-Wei Tian, Yuan Ren, Jin-Zhi Pei, Bai-Chao Ren, Yuan He
AIM: To investigate the potential of pigment epithelium-derived factor (PEDF) to protect the immortalized rat retinal ganglion cells-5 (RGC-5) exposed to CoCl2-induced chemical hypoxia. METHODS: After being differentiated with staurosporine (SS), RGC-5 cells were cultured in four conditions: control group cells cultured in Dulbecco's modified eagle medium (DMEM) supplemented with 10% fetal bovine serum, 100 µmol/mL streptomycin and penicillin (named as normal conditions); hypoxia group cells cultured in DMEM containing 300 µmol/mL CoCl2; cells in the group protected by PEDF were first pretreated with 100 ng/mL PEDF for 2h and then cultured in the same condition as hypoxia group cells; and PEDF group cells that were cultured in the presence of 100 ng/mL PEDF under normal conditions...
2017: International Journal of Ophthalmology
https://www.readbyqxmd.com/read/28728472/the-identification-of-interferon-%C3%AE-as-a-key-supportive-factor-for-retinal-differentiation-of-murine-mesenchymal-stem-cells
#2
Barbora Hermankova, Jan Kossl, Eliska Javorkova, Pavla Bohacova, Michaela Hajkova, Alena Zajicova, Magdalena Krulova, Vladimir Holan
Retinal disorders represent the main cause of decreased quality of vision and even blindness worldwide. The loss of retinal cells causes irreversible damage of the retina and there are currently no effective treatment protocols for most retinal degenerative diseases. A promising approach for the treatment of retinal disorders is represented by stem cell-based therapy. The perspective candidates are mesenchymal stem cells (MSCs) which can differentiate into multiple cell types and produce a number of trophic and growth factors...
July 20, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28726562/gene-therapy-for-age-related-macular-degeneration
#3
Nicholas A Moore, Peter Bracha, Rehan M Hussain, Nuria Morral, Thomas A Ciulla
In neovascular age related macular degeneration (nAMD), gene therapy to chronically express anti-vascular endothelial growth factor (VEGF) proteins could ameliorate the treatment burden of chronic intravitreal therapy and improve limited visual outcomes associated with 'real world' undertreatment. Areas covered: In this review, the authors assess the evolution of gene therapy for AMD. Adeno-associated virus (AAV) vectors can transduce retinal pigment epithelium; one such early application was a phase I trial of AAV2-delivered pigment epithelium derived factor gene in advanced nAMD...
July 20, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28706437/comparison-of-two-neurotrophic-serpins-reveals-a-small-fragment-with-cell-survival-activity
#4
Paige N Winokur, Preeti Subramanian, Jeanee L Bullock, Veronique Arocas, S Patricia Becerra
PURPOSE: Protease nexin-1 (PN-1), a serpin encoded by the SERPINE2 gene, has serine protease inhibitory activity and neurotrophic properties in the brain. PN-1 inhibits retinal angiogenesis; however, PN-1's neurotrophic capacities in the retina have not yet been evaluated. Pigment epithelium-derived factor (PEDF) is a serpin that exhibits neurotrophic and antiangiogenic activities but lacks protease inhibitory properties. The aim of this study is to compare PN-1 and PEDF. METHODS: Sequence comparisons were performed using computer bioinformatics programs...
2017: Molecular Vision
https://www.readbyqxmd.com/read/28698158/pigment-epithelium-derived-factor-play-a-positive-role-in-bone-mineralization-of-osteoblasts-derived-from-diabetic-patients
#5
Na Song, Jiateng Zhong, Jinghang Zhang, Jian Yu, Jinsong Li, Jinbo Qi, Jun Yang, Yanyan Qiu, Wei Su, Zhiwei Feng, Haijun Wang
Pigment epithelium-derived factor (PEDF) is a multifunctional secreted protein which plays important role in anti-angiogenic, anti-tumorigenic, as well as involves in the metabolism and regeneration of bone. In this study, our aim is to investigate the role of PEDF in regulating mineralization of osteoblasts from diabetic patients (DP). We isolated and cultured osteoblasts derived from DP and non-diabetic patients (NDP), in order to analyze the variable differences via gene expression and calcification assay in vitro...
July 8, 2017: Gene
https://www.readbyqxmd.com/read/28679845/negative-regulators-of-angiogenesis-important-targets-for-treatment-of-exudative-amd
#6
REVIEW
Mitra Farnoodian, Shoujian Wang, Joel Dietz, Robert W Nickells, Christine M Sorenson, Nader Sheibani
Angiogenesis contributes to the pathogenesis of many diseases including exudative age-related macular degeneration (AMD). It is normally kept in check by a tightly balanced production of pro- and anti-angiogenic factors. The up-regulation of the pro-angiogenic factor, vascular endothelial growth factor (VEGF), is intimately linked to the pathogenesis of exudative AMD, and its antagonism has been effectively targeted for treatment. However, very little is known about potential changes in expression of anti-angiogenic factors and the role they play in choroidal vascular homeostasis and neovascularization associated with AMD...
August 1, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28667418/diabetic-nephropathy-serum-mir-9-confers-a-poor-prognosis-in-and-is-associated-with-level-changes-of-vascular-endothelial-growth-factor-and-pigment-epithelium-derived-factor
#7
Yanxin Xiao, Shuqin Guo, Yunliang Zhang, Zhiying Bian, Liyan Jia, Yanyun Hu, Jie Chen, Chao Yin, Ning Li, Dongxun Zhang, Xincui Zhao, Jun Wang
OBJECTIVE: To investigate the relationship between the serum level of miR-9 and the progression of diabetic nephropathy (DN) and related molecular mechanisms. RESULTS: Thirty-five healthy subjects and 140 DN patients were divided into five groups: control, DN I-II, DN III, DN IV and DN V. Serum level of miR-9 was measured by real-time qPCR. Serum levels of vascular endothelial growth factor (VEGF), pigment epithelium-derived factor (PEDF) lipids, fasting glucose, insulin, hemoglobin A1c (HBA1c), creatinine, fibrinogen and insulin resistance (HOMA-IR) were also measured...
June 30, 2017: Biotechnology Letters
https://www.readbyqxmd.com/read/28664762/induced-pluripotent-stem-cell-based-therapy-for-age-related-macular-degeneration
#8
Peter Bracha, Nicholas A Moore, Thomas A Ciulla
In age-related macular degeneration (AMD), stem cells could possibly replace or regenerate disrupted pathologic retinal pigment epithelium (RPE), and produce supportive growth factors and cytokines such as brain-derived neurotrophic factor.  Induced pluripotent stem cells (iPSCs)-derived RPE was first subretinally transplanted in a neovascular AMD patient in 2014. Areas covered: Induced PSCs are derived from the introduction of transcription factors to adult cells under specific cell culture conditions, followed by differentiation into RPE cells...
June 30, 2017: Expert Opinion on Biological Therapy
https://www.readbyqxmd.com/read/28648555/comparison-of-plasma-pigment-epithelium-derived-factor-pedf-retinol-binding-protein-4-rbp-4-chitinase-3-like-protein-1-ykl-40-and-brain-derived-neurotrophic-factor-bdnf-for-the-identification-of-insulin-resistance
#9
F J K Toloza, M C Pérez-Matos, M L Ricardo-Silgado, M C Morales-Álvarez, J O Mantilla-Rivas, J A Pinzón-Cortés, M Pérez-Mayorga, M L Arévalo-García, G Tolosa-González, C O Mendivil
AIMS: To evaluate and compare the association of four potential insulin resistance (IR) biomarkers (pigment-epithelium-derived factor [PEDF], retinol-binding-protein-4 [RBP-4], chitinase-3-like protein 1 [YKL-40] and brain-derived neurotrophic factor [BDNF]) with objective measures of IR. METHODS: We studied 81 subjects with different metabolic profiles. All participants underwent a 5-point OGTT with calculation of multiple IR indexes. A subgroup of 21 participants additionally underwent a hyperinsulinemic-euglycemic clamp...
June 9, 2017: Journal of Diabetes and its Complications
https://www.readbyqxmd.com/read/28637898/activation-of-complement-by-pigment-epithelium-derived-factor-in-rheumatoid-arthritis
#10
Leonie M Vogt, Simone Talens, Ewa Kwasniewicz, Carsten Scavenius, André Struglics, Jan J Enghild, Tore Saxne, Anna M Blom
The aim of this study was to identify molecules that trigger complement activation in rheumatic joints. C4d, the final cleavage product of C4 activation, is found in the diseased joint and can bind covalently to complement-activating molecules. By using a highly specific Ab against a cleavage neoepitope in C4d, several molecules that were specifically bound to C4d were identified from pooled synovial fluid (SF) from four rheumatoid arthritis (RA) patients. One of these molecules, pigment epithelium-derived factor (PEDF), is a broadly expressed multifunctional member of the serine proteinase inhibitor family...
June 21, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28633944/gene-expression-analysis-identify-a-metabolic-and-cell-function-alterations-as-a-hallmark-of-obesity-without-metabolic-syndrome-in-peripheral-blood-a-pilot-study
#11
Daniel Antonio de Luis, Raquel Almansa, Rocío Aller, Olatz Izaola, E Romero
BACKGROUND: Understanding molecular basis involved in overweight is an important first step in developing therapeutic pathways against excess in body weight gain. OBJECTIVE: The purpose of our pilot study was to evaluate the gene expression profiles in the peripheral blood of obese patients without other metabolic complications. DESIGN: A sample of 17 obese patients without metabolic syndrome and 15 non obese control subjects was evaluated in a prospective way...
June 10, 2017: Clinical Nutrition: Official Journal of the European Society of Parenteral and Enteral Nutrition
https://www.readbyqxmd.com/read/28620131/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#12
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
June 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28602715/microstructure-dependent-binding-of-pigment-epithelium-derived-factor-pedf-to-type-i-collagen-fibrils
#13
Meagan Cauble, Phillip Yang, Ulrich Baumann, Jan M Gebauer, Bradford G Orr, Le T Duong, Mark M Banaszak Holl
Pigment epithelium derived factor (PEDF) is a multifunctional extracellular protein. In addition to its known anti-angiogenic and neurotrophic roles in collagen rich tissues, PEDF is thought to be involved in collagen fibril assembly due to its sequence specific binding to the collagen fibril and high expression in regions of active bone formation. In order to image the presence of the protein on the fibrils, PEDF was recombinantly made with a strep tag (strep-PEDF) and then gold nanoparticles conjugated to streptavidin (AuNP) were used as a secondary tag...
June 12, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28599441/effects-of-silencing-endothelin-1-on-invasion-and-vascular-formation-in-lung-cancer
#14
Zhen-Yu Zhang, Li-Li Chen, Wei Xu, Keshavraj Sigdel, Xing-Tang Jiang
Endothelin-1 (ET-1), which exists not only in the vascular endothelium but is also widely present in various tissues and cells, is an important cardiovascular regulatory factor that serves an important role in maintaining the basal vascular tone and homeostasis in the cardiovascular system. In the present study, the ET-1 gene was silenced by RNA interference, and the effects on lung cancer cell proliferation and tumor cell invasion were then detected by Cell Counting kit-8 and Transwell assays. In addition, the expression of apoptosis, growth and invasion-associated proteins, including RhoA/C, vascular endothelial growth factor, pigment epithelium-derived factor, AKT, E-cadherin and cyclooxygenase-2 was evaluated by western blotting upon silencing ET-1...
June 2017: Oncology Letters
https://www.readbyqxmd.com/read/28595552/pigment-epithelium-derived-factor-a-novel-therapeutic-target-for-cardiometabolic-diseases-and-related-complications
#15
Sho-Ichi Yamagishi, Takanori Matsui
Pigment epithelium-derived factor (PEDF) is a glycoprotein that belongs to the superfamily of serine protease inhibitors, serpins. It was first identified as a neuronal differentiating factor secreted by human retinal pigment epithelial cells, and then found to be the most potent inhibitor of pathological angiogenesis in mammalian eyes. Recently, PEDF has been shown not only to suppress oxidative stress and inflammatory reactions in vascular wall cells, T cells and macrophages, and adipocytes, but also to exert anti-thrombotic and anti-fibrotic properties, thereby protecting against the development and progression of various cardiometabolic diseases and related complications...
June 8, 2017: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/28592408/recovery-of-corneal-sensitivity-and-increase-in-nerve-density-and-wound-healing-in-diabetic-mice-after-pedf-plus-dha-treatment
#16
Jiucheng He, Thang Luong Pham, Azucena Kakazu, Haydee E P Bazan
Diabetic keratopathy decreases corneal sensation and tear secretion, and delays wound healing after injury. In the current study, we tested the effect of treatment with pigment epithelium-derived factor (PEDF) in combination with docosahexaenoic acid (DHA) on corneal nerve regeneration in a mouse model of diabetes with or without corneal injury. The study was performed in streptozotocin (STZ)-induced diabetic mice (C57BL/6). Ten weeks after STZ injection, diabetic mice showed significant decreases of corneal sensitivity, tear production and epithelial subbasal nerve density when compared with age-matched normal mice...
June 7, 2017: Diabetes
https://www.readbyqxmd.com/read/28583181/bringing-the-age-related-macular-degeneration-high-risk-allele-age-related-maculopathy-susceptibility-2-into-focus-with-stem-cell-technology
#17
REVIEW
Shuo Sun, ZhiQing Li, Patrick Glencer, BinCui Cai, XiaoMin Zhang, Jin Yang, XiaoRong Li
Age-related macular degeneration (AMD) is a major cause of blindness in older adults in developed countries. It is a multifactorial disease triggered by both environmental and genetic factors. High-temperature requirement A serine peptidase 1 (HTRA1) and age-related maculopathy susceptibility 2 (ARMS2) are two genes that are strongly associated with AMD. Because ARMS2 is an evolutionarily recent primate-specific gene and because the ARMS2/HTRA1 genes are positioned at a locus on chromosome 10q26 in a region with strong linkage disequilibrium, it is difficult to distinguish the functions of the individual genes...
June 6, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/28579317/six3-in-a-small-population-of-progenitors-at-e8-5-is-required-for-neuroretinal-specification-via-regulating-cell-signaling-and-survival-in-mice
#18
Wei Liu, Ales Cvekl
Neuroretina and retinal pigment epithelium (RPE) are differentiated from the progenitors in optic vesicles, but it is unclear when and how the two lineages are segregated. Manipulation of chick embryos reveals that the early anteroventral optic vesicle is crucial for neuroretinal development, but the molecular mechanism is unclear. Homeodomain transcription factor Six3 is required for neuroretinal specification and is dispensable for RPE formation, but the cell fates of Six3-deficient progenitors and the origins of remnant RPE are unknown...
August 1, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28569772/deficiency-of-pigment-epithelium-derived-factor-in-nasopharyngeal-carcinoma-cells-triggers-the-epithelial-mesenchymal-transition-and-metastasis
#19
Ting Zhang, Ping Yin, Zichen Zhang, Banglao Xu, Di Che, Zhiyu Dai, Chang Dong, Ping Jiang, Honghai Hong, Zhonghan Yang, Ti Zhou, Jianyong Shao, Zumin Xu, Xia Yang, Guoquan Gao
Distant metastasis is the primary cause of nasopharyngeal carcinoma (NPC) treatment failure while epithelial-mesenchymal transition (EMT) is the critical process of NPC invasion and metastasis. However, tumor-suppressor genes involved in the EMT and metastasis of NPC have not been explored clearly compared with the oncogenes. In the present study, the expression of pigment epithelium-derived factor (PEDF), a potent endogenous antitumor factor, was diminished in human NPC tissues and associated with clinicopathological and EMT features...
June 1, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28569253/enhanced-delivery-of-biodegradable-mpeg-plga-pll-nanoparticles-loading-cy3-labelled-pdgf-bb-sirna-by-utmd-to-rat-retina
#20
Jing DU, Ying Sun, Feng-Hua Li, Lian-Fang DU, You-Rong Duan
We investigated the efficacy and safety of ultrasound (US)-targeted microbubble (MB) destruction (UTMD)-enhanced delivery of monomethoxypoly(ethylene glycol)-poly(lactic-co-glycolic acid)-poly-L-lysine (mPEG-PLGA-PLL) nanoparticles (NPs) loading Cy3-labelled platelet-derived growth factor BB (PDGF-BB) siRNA to rat retina in vivo. Eighty Wistar rats were divided into five groups (G). The right eyes, respectively, received an intravitreal injection as follows: normal saline (NS) (G1), NPs and NS (G2), NPs and MBs (G3), NPs and NS (G4) and NPs and MBs (G5)...
June 2017: Journal of Biosciences
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