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Pigment epithelium derived factor

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https://www.readbyqxmd.com/read/29137305/mir-93-3p-inhibition-suppresses-clear-cell-renal-cell-carcinoma-proliferation-metastasis-and-invasion
#1
Lu Wang, Guang Yang, Xiangwei Zhu, Ziqi Wang, Hongzhi Wang, Yang Bai, Pengcheng Sun, Li Peng, Wei Wei, Guang Chen, Guangbin Li, Andrey A Zamyatnin, Peter V Glybochko, Wanhai Xu
miRNA dysregulation is associated with many human diseases, including cancer. This study explored the effects of miR-93-3p on clear cell renal cell carcinoma (ccRCC). We found that miR-93-3p is upregulated an average of 38-fold in 138 ccRCC specimens compared to matched normal kidney tissues, which correlated with poor patient outcome. miR-93-3p inhibition reduced ccRCC cell growth, invasion, and migration in vitro and in a mouse xenograft model. A search of the TargetScan, miRanda, and PicTar databases revealed that miR-93-3p is predicted to regulate pigment epithelium-derived factor (PEDF)...
October 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/29117277/naringenin-eye-drops-inhibit-corneal-neovascularization-by-anti-inflammatory-and-antioxidant-mechanisms
#2
Ana P M T Oguido, Miriam S N Hohmann, Felipe A Pinho-Ribeiro, Jefferson Crespigio, Talita P Domiciano, Waldiceu A Verri, Antonio M B Casella
Purpose: To investigate the effect of naringenin eye drops in corneal neovascularization induced by alkali (1 N NaOH) burn in mice. Methods: Corneal neovascularization in the right eye of male Swiss mice was induced by alkali. Treatment with naringenin eye drops (0.08-80 μg; 8 μL of 0.01-10 g/L solution) or vehicle (saline) started 2 days before corneal neovascularization was induced and was performed twice a day. Mice were treated up until the time animals were euthanized and cornea tissue was collected for testing, which was 2, 4, and 6 hours after alkali stimulus for cytokine and antioxidant capacity measurements, and 3 and/or 7 days after alkali stimulus for the assessment of corneal epithelial thickness and neovascularization, neutrophil, and macrophage recruitment, and vascular endothelial growth factor (Vegf), platelet-derived growth factor (Pdgf), matrix metalloproteinase-14 (Mmp14), and pigment epithelium-derived factor (Pedf) mRNA expression...
November 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29105655/pre-metastatic-cancer-exosomes-induce-immune-surveillance-by-patrolling-monocytes-at-the-metastatic-niche
#3
Michael P Plebanek, Nicholas L Angeloni, Elena Vinokour, Jia Li, Anna Henkin, Dalia Martinez-Marin, Stephanie Filleur, Reshma Bhowmick, Jack Henkin, Stephen D Miller, Igal Ifergan, Yesung Lee, Iman Osman, C Shad Thaxton, Olga V Volpert
Metastatic cancers produce exosomes that condition pre-metastatic niches in remote microenvironments to favor metastasis. In contrast, here we show that exosomes from poorly metastatic melanoma cells can potently inhibit metastasis to the lung. These "non-metastatic" exosomes stimulate an innate immune response through the expansion of Ly6C(low) patrolling monocytes (PMo) in the bone marrow, which then cause cancer cell clearance at the pre-metastatic niche, via the recruitment of NK cells and TRAIL-dependent killing of melanoma cells by macrophages...
November 6, 2017: Nature Communications
https://www.readbyqxmd.com/read/29104038/extremely-low-level-of-serum-pigment-epithelium-derived-factor-is-a-special-biomarker-of-chinese-osteogenesis-imperfecta-patients-with-serpinf1-mutations
#4
Jian-Yi Wang, Lu-Jiao Li, Qian Zhang, Yi Liu, Fang Lv, Xiao-Jie Xu, Yu-Wen Song, Ou Wang, Yan Jiang, Wei-Bo Xia, Xiao-Ping Xing, Mei Li
BACKGROUNDS: SERPINF1 mutations caused deficiency of pigment epithelium-derived factor (PEDF) and would lead to osteogenesis imperfecta (OI) type VI. However, serum PEDF levels were unclear in Chinese OI patients who had clear molecular diagnosis. OBJECTIVE: To assess PEDF levels in different genotypes of OI, to evaluate the influencing factors of PEDF in Chinese OI patients with clear molecular diagnosis. METHODS: Known candidate genes of OI were examined by a targeted next generation sequence...
November 2, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29100284/exosomes-containing-differential-expression-of-microrna-and-mrna-in-osteosarcoma-that-can-predict-response-to-chemotherapy
#5
Ji-Feng Xu, Ya-Ping Wang, Shui-Jun Zhang, Yu Chen, Hai-Feng Gu, Xiao-Fan Dou, Bing Xia, Qing Bi, Shun-Wu Fan
A major challenge in osteosarcoma (OS) is the selection of the most effective chemotherapeutic agents for individual patients, while the administration of ineffective chemotherapy increases mortality and decreases quality of life in patients. This emphasizes the need to evaluate every patient's probability of responding to each chemotherapeutic agent. We developed a profiling strategy for serum exosomal microRNAs and mRNAs in OS patients with differential chemotherapeutic responses. Twelve miRNAs were up regulated and 18 miRNAs were under regulated significantly in OS patient with poor chemotherapeutic response when compared with those in good chemotherapeutic response (p<0...
September 29, 2017: Oncotarget
https://www.readbyqxmd.com/read/29075761/corneal-mesenchymal-stromal-cells-are-directly-antiangiogenic-via-pedf-and-sflt-1
#6
Medi Eslani, Ilham Putra, Xiang Shen, Judy Hamouie, Neda Afsharkhamseh, Soroush Besharat, Mark I Rosenblatt, Reza Dana, Peiman Hematti, Ali R Djalilian
Purpose: To evaluate the angiogenic properties of corneal derived mesenchymal stromal cells (Co-MSC). Methods: Co-MSCs were extracted from human cadaver, and wild-type (C57BL/6J) and SERPINF1-/- mice corneas. The MSC secretome was collected in a serum-free medium. Human umbilical vein endothelial cell (HUVEC) tube formation and fibrin gel bead assay (FIBA) sprout formation were used to assess the angiogenic properties of Co-MSC secretome. Complete corneal epithelial debridement was used to induce corneal neovascularization in wild-type mice...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29050001/expression-of-angiogenic-and-inflammatory-factors-in-choroidal-neovascularisation-derived-retinal-pigment-epithelium
#7
Christoph Ehlken, Maria-Magdalena Guichard, Günther Schlunck, Anima D Bühler, Gottfried Martin, Hansjürgen T Agostini
PURPOSE: Anti-angiogenic treatment is well established in the management of exudative age-related macular degeneration (AMD), but not sufficient in all patients. The characterisation of factors driving this chronic disease could serve to identify additional treatment options. The purpose of this study was to assess gene expression patterns and distinct changes in cells derived from surgically extracted choroidal neovascularisation (CNV) membranes. MATERIALS AND METHODS: The expression of >11,000 genes was analysed by means of a microarray in cells cultured from 2 late-stage CNV membranes compared to primary human retinal pigment epithelium (RPE) and ARPE-19 cells...
October 20, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/29042415/long-term-effects-of-weight-loss-exercise-on-biomarkers-associated-with-angiogenesis
#8
Catherine Duggan, Jean de Dieu Tapsoba, Ching-Yun Wang, Karen E Foster-Schubert, Anne McTiernan
BACKGROUND: We tested the effect of weight-loss on circulating levels of the angiogenic factors vascular endothelial growth factor (VEGF), and pigment epithelium-derived factor (PEDF) in postmenopausal overweight/obese women, 18 months after completing a year-long 4-arm randomized controlled trial of behavioral weight-loss and/or exercise vs. control (i.e., 30-months post-randomization). METHODS: 439 overweight/obese, postmenopausal women, 50-75 years, were randomized to: diet (goal: 10% weight-loss, N=118), exercise (225 min/week moderate-to-vigorous activity, N=117), diet+exercise (N=117), or control (N=87)...
October 17, 2017: Cancer Epidemiology, Biomarkers & Prevention
https://www.readbyqxmd.com/read/29022192/a-positive-circuit-of-vegf-increases-glut-1-expression-by-increasing-hif-1%C3%AE-gene-expression-in-human-retinal-endothelial-cells
#9
Yoon Kyung Choi
Treatment of human retinal microvascular endothelial cells (HRMECs) with vascular endothelial growth factor 165 (VEGF165) increased hypoxia-inducible factor 1α (HIF-1α), VEGF, and glucose transporter 1 (Glut-1) mRNA expression and Glut-1 protein localization to the membrane. In contrast, treatment of human retinal pigment epithelium cells with VEGF165 did not induce HIF-1α, VEGF, and Glut-1 gene expression. Microvascular endothelial cells are surrounded by astrocytic end feet in the retina. Astrocyte-derived A-kinase anchor protein 12 overexpression during hypoxia downregulated VEGF secretion, and this conditioned medium reduced VEGF and Glut-1 expression in HRMECs, suggesting that communications between astrocytes and endothelial cells may be the determinants of the blood vessel network...
October 11, 2017: Archives of Pharmacal Research
https://www.readbyqxmd.com/read/28972155/defining-a-mechanistic-link-between-pigment-epithelium-derived-factor-docosahexaenoic-acid-and-corneal-nerve-regeneration
#10
Thang Luong Pham, Jiucheng He, Azucena Kakazu, Bokkyoo Jun, Nicolas G Bazan, Haydee E P Bazan
The cornea is densely innervated to sustain the integrity of the ocular surface. Corneal nerve damage produced by aging, diabetes, refractive surgeries and viral or bacterial infections impairs tear production, the blinking reflex and epithelial wound healing, resulting in loss of transparency and vision. A combination of the known neuroprotective molecule, pigment epithelium-derived factor (PEDF) plus docosahexaenoic acid (DHA), has been shown to stimulate corneal nerve regeneration, but the mechanisms involved are unclear...
September 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28964579/clinicopathologic-correlation-of-anti-vascular-endothelial-growth-factor-treated-type-3-neovascularization-in%C3%A2-age-related-macular-degeneration
#11
Miaoling Li, Rosa Dolz-Marco, Jeffrey D Messinger, Lan Wang, Richard M Feist, Christopher A Girkin, Sarra Gattoussi, Daniela Ferrara, Christine A Curcio, K Bailey Freund
PURPOSE: To correlate histologic results with previously recorded multimodal imaging results from a patient with type 3 neovascularization secondary to age-related macular degeneration (AMD). DESIGN: Case study, clinical imaging, laboratory imaging, and eye-tracked clinicopathologic correlation. PARTICIPANT: An 86-year-old white woman with type 3 neovascularization secondary to AMD treated with 6 intravitreal injections of bevacizumab. METHODS: Multimodal retinal imaging at each clinic visit was correlated with ex vivo and high-resolution histologic images of the preserved donor eye...
September 27, 2017: Ophthalmology
https://www.readbyqxmd.com/read/28951365/-expression-of-vegf-and-pedf-in-early-stage-retinopathy-in-diabetic-macaca-mulatta
#12
Jing-Fei Chen, Qi-Hui Luo, Chao Huang, Wen-Tao Liu, Wen Zeng, Qi Gao, Ping Chen, Bing Chen, Zheng-Li Chen
OBJECTIVE: To explore the expression of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF) in early stage of diabetic retinopathy (DR) in Macaca mulatta. METHODS: Three diabetic Macaca mulattas induced by high-fat diet were identified for early stage of diabetic retinopathy according to the fasting plasma glucose, hemoglobin Alc (HbA1c), fundus photograph and duration of diabetes, with another 3 age-matched healthy Macaca mulattas as control...
September 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28944893/pigment-epithelium%C3%A2-derived-factor-protects-human-glomerular-mesangial-cells-from-diabetes-via-noxo1%C3%A2-inos-suppression
#13
Ling Gao, Yingjie Hu, Jing Li
The authors previously reported that pigment epithelium‑derived factor (PEDF) protects the diabetic kidney from fibrosis via its anti‑oxidative effects. However, the underlying molecular mechanism has never been revealed. The present study aimed to investigate how PEDF protects mesangial cells from diabetes induced damage. Human mesangial cells were exposed to a diabetic environment [30 mmol/l glucose and 200 mg advanced glycation end products (AGEs)] in the absence or presence of PEDF (200 mg/l). The superoxide and peroxynitrite productions were measured by fluorescent assay...
November 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28913923/an-induced-pluripotent-stem-cell-patient-specific-model-of-complement-factor-h-y402h-polymorphism-displays-characteristic-features-of-age-related-macular-degeneration-and-indicates-a-beneficial-role-for-uv-light-exposure
#14
Dean Hallam, Joseph Collin, Sanja Bojic, Valeria Chichagova, Adriana Buskin, Yaobo Xu, Lucia Lafage, Elsje G Otten, George Anyfantis, Carla Mellough, Stefan Przyborski, Sameer Alharthi, Viktor Korolchuk, Andrew Lotery, Gabriele Saretzki, Martin McKibbin, Lyle Armstrong, David Steel, David Kavanagh, Majlinda Lako
Age-related macular degeneration (AMD) is the most common cause of blindness, accounting for 8.7% of all blindness globally. Vision loss is caused ultimately by apoptosis of the retinal pigment epithelium (RPE) and overlying photoreceptors. Treatments are evolving for the wet form of the disease; however, these do not exist for the dry form. Complement factor H polymorphism in exon 9 (Y402H) has shown a strong association with susceptibility to AMD resulting in complement activation, recruitment of phagocytes, RPE damage, and visual decline...
November 2017: Stem Cells
https://www.readbyqxmd.com/read/28911166/hormonal-and-growth-regulation-of-epithelial-and-stromal-cells-from-the-normal-and-malignant-endometrium-by-pigment-epithelium-derived-factor
#15
Julien Daubriac, Unnati M Pandya, Kuang-Tzu Huang, Savvas C Pavlides, Patricia Gama, Stephanie V Blank, Pratibha Shukla, Susan E Crawford, Leslie I Gold
We discovered that pigment epithelium-derived factor (PEDF)-null mice have endometrial hyperplasia, the precursor to human type I endometrial cancer (ECA), which is etiologically linked to unopposed estrogen (E2), suggesting that this potent antiangiogenic factor might contribute to dysregulated growth and the development of type I ECA. Treatment of both ECA cell lines and primary ECA cells with recombinant PEDF dose dependently decreased cellular proliferation via an autocrine mechanism by blocking cells in G1 and G2 phases of the cell cycle...
September 1, 2017: Endocrinology
https://www.readbyqxmd.com/read/28886036/potential-role-of-sirtuin-1-in-m%C3%A3-ller-glial-cells-in-mice-choroidal-neovascularization
#16
Tomoka Ishida, Takeshi Yoshida, Kosei Shinohara, Kejia Cao, Ken-Ichi Nakahama, Ikuo Morita, Kyoko Ohno-Matsui
This study investigated the potential role of sirtuin 1 in Müller glial cells in choroidal neovascularization. In the in vitro study, primary Müller glial cells were cultured and treated with resveratrol, a sirtuin 1 activator. Glial fibrillary acidic protein expression and angiogenesis-related gene expression were examined using quantitative polymerase chain reaction and phagocytosis, as a marker of Müller glial cell function; in addition, a latex bead assay was used to analyze cell function. For the in vivo study, choroidal neovascularization was induced in C57BL/6 mice via laser photocoagulation, and resveratrol was administered intravitreally...
2017: PloS One
https://www.readbyqxmd.com/read/28880276/lncrna-znf503-as1-promotes-rpe-differentiation-by-downregulating-znf503-expression
#17
Xue Chen, Chao Jiang, Bing Qin, Guohua Liu, Jiangdong Ji, Xiantao Sun, Min Xu, Sijia Ding, Meidong Zhu, Guofu Huang, Biao Yan, Chen Zhao
Long noncoding RNAs (lncRNAs) have important roles in various biological processes. Our previous work has revealed that dedifferentiation of retinal pigment epithelium (RPE) cells contributes to the pathology of age-related macular degeneration (AMD). Herein, we show roles of lncRNAs in RPE differentiation. We used microarray to identify lncRNA expression profiles in human induced pluripotent stem cells (hiPSCs) and hiPSC-derived RPE cells. A total of 217 differentially expressed lncRNAs along with the differentiation were initially identified, among which 13 lncRNAs showed a consistent fold change of over 2...
September 7, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28860613/the-stem-cell-regulator-pedf-is-dispensable-for-maintenance-and-function-of-hematopoietic-stem-cells
#18
Emma Rörby, Matilda Billing, Maria Dahl, Sarah Warsi, Silja Andradottir, Kenichi Miharada, Kavitha Siva, Jan-Ingvar Jönsson, Ulrika Blank, Göran Karlsson, Stefan Karlsson
Pigment epithelium derived factor (PEDF), a ubiquitously expressed 50 kDa secreted glycoprotein, was recently discovered to regulate self-renewal of neural stem cells and have a supportive effect on human embryonic stem cell growth. Here, we analyzed expression of PEDF in the murine hematopoietic stem cell (HSC) compartments and found that PEDF is highly expressed in primary long-term HSCs. Therefore, we characterized the hematopoietic system in a knockout mouse model for PEDF and using this model we surprisingly found that PEDF is dispensable for HSC regulation...
August 31, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28837677/characterization-of-lincrna-expression-in-the-human-retinal-pigment-epithelium-and-differentiated-induced-pluripotent-stem-cells
#19
Elizabeth D Au, Rosario Fernandez-Godino, Tadeusz J Kaczynksi, Maria E Sousa, Michael H Farkas
Long intervening non-coding RNAs (lincRNAs) are increasingly being implicated as important factors in many aspects of cellular development, function, and disease, but remain poorly understood. In this study, we examine the human retinal pigment epithelium (RPE) lincRNA transcriptome using RNA-Seq data generated from human fetal RPE (fRPE), RPE derived from human induced pluripotent stem cells (iPS-RPE), and undifferentiated iPS (iPS). In addition, we determine the suitability of iPS-RPE, from a transcriptome standpoint, as a model for use in future studies of lincRNA structure and function...
2017: PloS One
https://www.readbyqxmd.com/read/28835854/retinal-and-choroidal-angiogenesis-a-review-of-new-targets
#20
REVIEW
Thiago Cabral, Luiz Guilherme M Mello, Luiz H Lima, Júlia Polido, Caio V Regatieri, Rubens Belfort, Vinit B Mahajan
Retinal and choroidal neovascularization are a major cause of significant visual impairment, worldwide. Understanding the various factors involved in the accompanying physiopathology is vital for development of novel treatments, and most important, for preserving patient vision. The intraocular use of anti-vascular endothelial growth factor therapeutics has improved management of the retinal and choroidal neovascularization but some patients do not respond, suggesting other vascular mediators may also contribute to ocular angiogenesis...
2017: International Journal of Retina and Vitreous
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