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https://www.readbyqxmd.com/read/29792916/flow-cytometry-based-method-facilitates-optimization-of-pma-treatment-condition-for-pma-qpcr-method
#1
Zhiqing Huang, Jianwei Zheng, Chunmei Shi, Qiang Chen
Coupling propidium monoazide (PMA) with quantitative PCR (PMA-qPCR) has been successfully applied to specific detection and quantification of viable cells in various samples. The optimal PMA treatment condition is usually determined through qPCR. However, it is a tedious, time consuming and costly process including DNA extraction and qPCR. To overcome this problem, a flow cytometry-based (FCM-based) method was first proposed in this study to replace qPCR for screening of the optimal PMA treatment condition for Helicobacter pylori, since the pure culture treated with PMA was actually a single cell suspension with fluorescent dye...
May 21, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29791423/-helicobacter-pylori-how-do-we-treat-it-in-2018
#2
William Otero R, Martín Gómez Z, Lina Otero P, Alba Trespalacios R
This work is a review on the basic aspects of the treatment of Helicobacter pylori, highlighting the causes of treatment failure and strategies exist to optimize the treatment according to the best evidence posted. Stands out the antimicrobial resistance as the main cause of treatment failure, as well as the different compartments where the microorganism is hosted. Shows major schemes currently available and how to choose therapies first, second, third line and rescue therapies.
January 2018: Revista de Gastroenterología del Perú: órgano Oficial de la Sociedad de Gastroenterología del Perú
https://www.readbyqxmd.com/read/29788098/helicobacter-pylori-colonization-protects-against-chronic-experimental-colitis-by-regulating-th17-treg-balance
#3
Hongchen Zhang, Yun Dai, Yun Liu, Ting Wu, Jing Li, Xiaolei Wang, Weihong Wang
Background: Epidemiological studies have demonstrated an inverse association between Helicobacter pylori infection and the risk of developing inflammatory bowel disease (IBD). The mechanisms by which H. pylori infection protects against IBD are unclear. Here, we explored the possible protective effects and mechanisms of gastric H. pylori colonization on a chronic colitis model, with focus on whether H. pylori exerted its effects through regulating Th17/Treg immune responses. Methods: Chronic colitis was induced by dextran sulfate sodium (DSS) treatment...
May 17, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29786110/multi%C3%A2-layered-prevention-and-treatment-of-chronic-inflammation-organ-fibrosis-and-cancer-associated-with-canonical-wnt-%C3%AE-%C3%A2-catenin-signaling-activation-review
#4
Masaru Katoh
β‑catenin/CTNNB1 is an intracellular scaffold protein that interacts with adhesion molecules (E‑cadherin/CDH1, N‑cadherin/CDH2, VE‑cadherin/CDH5 and α‑catenins), transmembrane‑type mucins (MUC1/CD227 and MUC16/CA125), signaling regulators (APC, AXIN1, AXIN2 and NHERF1/EBP50) and epigenetic or transcriptional regulators (BCL9, BCL9L, CREBBP/CBP, EP300/p300, FOXM1, MED12, SMARCA4/BRG1 and TCF/LEF). Gain‑of‑function CTTNB1 mutations are detected in bladder cancer, colorectal cancer, gastric cancer, liver cancer, lung cancer, pancreatic cancer, prostate cancer and uterine cancer, whereas loss‑of‑function CTNNB1 mutations are also detected in human cancer...
May 17, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785564/helicobacter-pylori-infection-in-pediatric-patients-update-on-diagnosis-and-eradication-strategies
#5
REVIEW
Kallirroi Kotilea, Nicolas Kalach, Matjaž Homan, Patrick Bontems
Helicobacter pylori infection is acquired mainly in childhood and remains an essential cause of peptic ulcer disease and gastric cancer. This article provides commentary on the last ESPGHAN/NASPGHAN guidelines and on publications made after the consensus conference of 2015. The majority of infected children are asymptomatic and pediatric studies do not support a role for H. pylori in functional disorders such as recurrent abdominal pain. The role of H. pylori infection in failure to thrive, children's growth, type I diabetes mellitus (T1DM), and celiac disease remains controversial...
May 22, 2018: Paediatric Drugs
https://www.readbyqxmd.com/read/29779412/primary-gastric-lymphoma-epidemiology-clinical-diagnosis-and-treatment
#6
Luis Miguel Juárez-Salcedo, Lubomir Sokol, Julio C Chavez, Samir Dalia
Primary gastric lymphoma (PGL) is the most common extranodal non-Hodgkin lymphoma and represents a wide spectrum of disease, ranging from indolent low-grade marginal zone lymphoma or mucosa-associated lymphoid tissue (MALT) lymphoma to aggressive diffuse large B-cell lymphoma. The PGL is a relatively rare cancer and easily misdiagnosed due to its unspecific symptoms of the digestive tract. The medical literature and ongoing clinical trials were reviewed on the clinical presentation, diagnosis, prognosis, prevention, and treatment of PGL...
January 2018: Cancer Control: Journal of the Moffitt Cancer Center
https://www.readbyqxmd.com/read/29774942/-progress-and-controversy-on-diagnosis-and-treatment-of-gastric-stump-cancer
#7
Zhidong Gao, Yongbai Li, Kewei Jiang, Yingjiang Ye, Shan Wang
Gastric stump cancer (GSC) is a carcinoma arising from the remnant stomach following gastric surgery for benign or malignant disease, and is more common in men. The risk of morbidity has an obvious time dependence. GSC incidence is likely to rise with lengthening of the initial operation interval. The GSC time interval after malignant disease is significantly shorter than that of benign disease. GSC etiologies mainly include duodenogastric reflux and denervation of the gastric mucosa resulting in the change of the gastric environment after gastrectomy and the Helicobacter pylori infection...
May 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/29774929/-epidemiological-characteristics-and-inducing-factors-of-gastric-stump-cancer
#8
Lin Chen
Gastric stump cancer was initially defined as a carcinoma of the stomach occurring more than 5 years after surgery for gastric or duodenal benign disease. In recent years, as the number of total gastrectomy for benign disease has gradually decreased and the gastric cancer detection and operation rate have annually increased, residual recurrence of primary gastric cancer more than 10 years after gastric cancer surgery has also been considered as gastric stump cancer. The incidence of gastric stump cancer is increasing annually...
May 25, 2018: Zhonghua Wei Chang Wai Ke za Zhi, Chinese Journal of Gastrointestinal Surgery
https://www.readbyqxmd.com/read/29774152/acquisition-of-double-mutation-in-gyra-caused-high-resistance-to-sitafloxacin-in-helicobacter-pylori-after-unsuccessful-eradication-with-sitafloxacin-containing-regimens
#9
Hideki Mori, Hidekazu Suzuki, Juntaro Matsuzaki, Tatsuhiro Masaoka, Takanori Kanai
Background and aim: Although sitafloxacin (STFX)-containing regimens are effective rescue treatments for Helicobacter pylori infection, prevalence of fluoroquinolone resistance in H. pylori has increased rapidly worldwide. The change in resistance levels and gyrA mutations, a major cause of fluoroquinolone resistance, after unsuccessful STFX-containing treatment has not been investigated. Methods: We conducted a retrospective, non-randomized study to compare the minimum inhibitory concentrations (MICs) of STFX and the location of gyrA mutations in H...
April 2018: United European Gastroenterology Journal
https://www.readbyqxmd.com/read/29773406/dynamics-of-helicobacter-pylori-infection-in-infants-during-the-first-six-months-of-life
#10
José S Merino, Liliana Araneda, Paulina Lincoñir-Campos, Cristian Parra, Katia Sáez, Apolinaria García
INTRODUCTION: Mainly due to the high percentage of infection and the ineffectiveness of treatments, Helicobacter pylori is a global health problem. Knowing the age at acquisition is key to preventing the infection. PATIENTS: Sixty-seven mothers and their respective children participated. To evaluate the presence of H. pylori, the faeces of the mother and her child were analysed using the HpSA antigen test. RESULTS: 71.6% (48/67) of pregnant mothers were positive for H...
May 14, 2018: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/29768551/risk-factors-of-helicobacter-pylori-infection-in-an-urban-community-in-northeast-brazil-and-the-relationship-between-the-infection-and-gastric-diseases
#11
Irigrácin Lima Diniz Basílio, Maria de Fátima Celino Catão, Janilson Dantas de Sousa Carvalho, Francisco Paulo Freire-Neto, Leonardo Capristano Ferreira, Selma Maria Bezerra Jerônimo
INTRODUCTION: Helicobacter pylori, a water contaminant, is the primary pathogenic agent associated with gastric diseases in humans. Exposure to H. pylori is more likely higher in developing countries. This study aimed to evaluate the risk factors associated with H. pylori infection in patients undergoing endoscopy to validate the cause of dyspeptic symptoms in an urban population in northeast Brazil and to compare the urease test and polymerase chain reaction assay results with the histopathological findings...
March 2018: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/29768373/acute-myelitis-of-children-with-positive-anti-gm1-antibody-case-series-and-literature-review
#12
Zhiling Wang, Di Qie, Hui Zhou, Xiao Tang Cai
RATIONALE: To explore the clinical features, treatment, and prognosis of acute myelitis (AM) of children with positive blood anti- ganglioside (GM1) antibodies. PATIENT CONCERNS: Two cases of AM of children with positive anti-GM1 antibody were retrospectively collected and followed up for 6 months. Two cases had positive helicobacter pylori IgG antibody, and Case 2 also had positive mycoplasma IgM antibody. DIAGNOSES: Two cases had typical symptoms of myelitis, abnormal spinal magnetic resonance imaging (MRI), and positive serum anti-GM1 IgM...
May 2018: Medicine (Baltimore)
https://www.readbyqxmd.com/read/29762682/molecular-detection-of-mutations-involved-in-helicobacter-pylori-antibiotic-resistance-in-algeria
#13
Meryem Bachir, Rachida Allem, Lucie Benejat, Abedelkarim Tifrit, Meriem Medjekane, Amine El-Mokhtar Drici, Francis Megraud, Kara Turki Douidi
Objectives: In Algeria, there are limited data regarding the pattern of Helicobacter pylori primary antibiotic resistance. The aim of this study was to evaluate the primary resistance of H. pylori to clarithromycin, ciprofloxacin, tetracycline and rifampicin and to determine the molecular mechanisms involved in the resistance. Methods: Two hundred and seventy Algerian adults who had never received H. pylori treatment were enrolled in this study. Human biopsies were obtained for culture and antimicrobial susceptibility testing was performed by Etest for clarithromycin, ciprofloxacin, tetracycline and rifampicin...
May 11, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29760614/eradication-of-helicobacter-pylori-as-add-on-therapy-has-a-significant-but-temporary-influence-on-recovery-in-chronic-idiopathic-urticaria-a-placebo-controlled-double-blind-trial-in-the-polish-population
#14
Robert Pawłowicz, Krzysztof Wytrychowski, Bernard Panaszek
Introduction: The infectious factor like Helicobacter pylori (HP) has been thought to trigger the vicious circle of chronic idiopathic urticaria (CIU), therefore its eradication could modify the course of the disease. Aim: To reveal the influence of HP eradication on CIU clinical course. Material and methods: Sixty-four CIU patients, receiving fexofenadine, as the basic treatment, took part in the research, divided into 3 groups: HP patients treated by eradication, HP patients receiving placebo, and patients without bacteria...
April 2018: Postȩpy Dermatologii i Alergologii
https://www.readbyqxmd.com/read/29743338/helicobacter-pylori-biofilm-formation-and-its-potential-role-in-pathogenesis
#15
REVIEW
Skander Hathroubi, Stephanie L Servetas, Ian Windham, D Scott Merrell, Karen M Ottemann
Despite decades of effort, Helicobacter pylori infections remain difficult to treat. Over half of the world's population is infected by H. pylori , which is a major cause of duodenal and gastric ulcers as well as gastric cancer. During chronic infection, H. pylori localizes within the gastric mucosal layer, including deep within invaginations called glands; thanks to its impressive ability to survive despite the harsh acidic environment, it can persist for the host's lifetime. This ability to survive and persist in the stomach is associated with urease production, chemotactic motility, and the ability to adapt to the fluctuating environment...
June 2018: Microbiology and Molecular Biology Reviews: MMBR
https://www.readbyqxmd.com/read/29729504/new-food-approaches-to-reduce-and-or-eliminate-increased-gastric-acidity-related-to-gastroesophageal-pathologies
#16
Ciro Langella, Daniele Naviglio, Marina Marino, Armando Calogero, Monica Gallo
OBJECTIVE: Gastroesophageal reflux disease is very common in industrialized countries and rapidly and significantly increasing even in developing countries. The approach in this study is one not commonly found to date in the scientific literature. To assess the ability of reduced-carbohydrate diets and foods that are enriched with acid potential of hydrogen (pH; lemon and tomato) to quickly and exponentially reduce symptoms that are related to conditions such as gastritis and gastroesophageal reflux and unrelated to Helicobacter pylori...
March 22, 2018: Nutrition
https://www.readbyqxmd.com/read/29729058/a-case-of-pyloric-gland-adenoma-accompanied-by-a-component-of-foveolar-epithelial-type-adenoma-within-the-lesion
#17
Keiichiro Nakajo, Yasuhiro Oono, Takeshi Kuwata
An 80-year-old woman found to have a gastric tumor by endoscopy was referred to our hospital for further examination and treatment. Helicobacter pylori eradication therapy was successfully performed 10 years ago. Conventional endoscopy revealed a 20-mm flat elevated lesion in the greater curvature of the middle gastric body. (Fig. 1a). Magnifying endoscopy with narrow band imaging (M-NBI) revealed an irregular microvascular architecture, including closed-loop vessels with repeated irregular anastomoses (Fig...
May 5, 2018: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
https://www.readbyqxmd.com/read/29728019/-molecular-mechanism-of-mylabris-for-colorectal-cancer-based-on-integrative-pharmacology
#18
Lei Yang, Meng-Jiao Liu, Zhi-Guo Zhang, Wen-Ming Chen, Qin Zhang, Lin Ouyang, Ping Wu
At present, the disjointing situation is generally present between "systemic"and "local", "macro" and "micro", " in vivo process" and "activity evaluation" in the study of traditional Chinese medicine (TCM). An urgent task for the modernization of TCM is to establish new strategies and methods which can reflect the overall characteristics of TCM. The introduction of integrative pharmacology provided a feasible approach to solve the problem of the fragmentation of TCM...
April 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29727437/evaluation-of-helicobacter-pylori-infection-and-clarithromycin-resistance-in-strains-from-symptomatic-colombian-children
#19
Yuliet Liliana Rosero Lasso, Betsy Verónica Arévalo-Jaimes, María de Pilar Delgado, José Fernando Vera-Chamorro, Daniella García, Andrea Ramírez, Paula A Rodríguez-Urrego, Johanna Álvarez, Carlos Alberto Jaramillo
OBJECTIVES: To determine the current prevalence of Helicobacter pylori in symptomatic Colombian children and evaluate the presence of mutations associated with clarithromycin resistance. PATIENTS AND METHODS: Biopsies from 133 children were analyzed. The gastric fragment was used for urease test and reused for PCR-sequencing of the 23SrDNA gene. Mutations were detected by bioinformatic analysis. RESULTS: PCR-sequencing established that H. pylori infection was present in 47% of patients...
April 27, 2018: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/29724084/-helicobacter-pylori-eradication-in-the-treatment-of-gastric-hyperplastic-polyps-beyond-national-health-insurance
#20
Jin Sung Koh, Moon Kyung Joo
No abstract text is available yet for this article.
May 2018: Korean Journal of Internal Medicine
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