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MRSA rat

Jinxiao Guo, Huaijuan Zhou, Jiaxing Wang, Wei Liu, Mengqi Cheng, Xiaochun Peng, Hui Qin, Jianfeng Wei, Ping Jin, Jinhua Li, Xianlong Zhang
Vanadium is a trace element in the human body, and vanadium compounds have a promising future in biological and medical applications due to their various biological activities and low toxicity. Herein, a novel pure vanadium dioxide (VO2 ) nanofilm was deposited on a substrate of biomedical titanium by magnetron sputtering. The antibacterial effect of VO2 against the methicillin-resistant Staphylococcus aureus (MRSA) was validated in vitro and in vivo. Moreover, the biocompatibility of VO2 and its osteogenic effects were systematically illustrated...
March 21, 2018: Artificial Cells, Nanomedicine, and Biotechnology
Jamie L Rothenburger, Chelsea G Himsworth, Nicole M Nemeth, David L Pearl, Claire M Jardine
Worldwide, Norway rats (Rattus norvegicus) carry a number of zoonotic pathogens. Many studies have identified rat-level risk factors for pathogen carriage. The objective of this study was to examine associations between abundance, microenvironmental and weather features and Clostridium difficile, antimicrobial-resistant (AMR) Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) carriage in urban rats. We assessed city blocks for rat abundance and 48 microenvironmental variables during a trap-removal study, then constructed 32 time-lagged temperature and precipitation variables and fitted multivariable logistic regression models...
February 9, 2018: EcoHealth
Mercedeh Tajbakhsh, Abdollah Karimi, Abolghasem Tohidpour, Naser Abbasi, Fatemeh Fallah, Maziar Mohammad Akhavan
Cathelicidins are a family of antimicrobial peptides which exhibit broad antimicrobial activities against antibiotic-resistant bacteria. Considering the progressive antibiotic resistance, cathelicidin is a candidate for use as an alternative approach to treat and overcome the challenge of antimicrobial resistance. Cathelicidin-BF (Cath-BF) is a short antimicrobial peptide, which was originally extracted from the venom of Bungarus fasciatus. Recent studies have reported that Cath-BF and some related derivatives exert strong antimicrobial and weak hemolytic properties...
February 2018: Journal of Microbiology / the Microbiological Society of Korea
Jamie L Rothenburger, Joyce D Rousseau, J Scott Weese, Claire M Jardine
Methicillin-resistant Staphylococcus aureus (MRSA) and Clostridium difficile are important human pathogens that are also carried by animals. The role of wild mammals on farms in their maintenance and transmission, however, is poorly understood. To determine if Norway rats (Rattus norvegicus) are potential carriers of these bacteria on Canadian farms, we tested 21 rats from swine farms in Ontario. The MRSA spa type t034 was isolated from 1 (4.8%) rat. This livestock-associated strain often colonizes pigs and pig farmers, suggesting that transmission among rats and pigs or environmental transmission is possible on pig farms...
January 2018: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
Cagri Neyisci, Yusuf Erdem, Ahmet Burak Bilekli, Bahtiyar Demiralp, Ozkan Kose, Dogan Bek, Feza Korkusuz, Berna Kankilic
INTRODUCTION: The purpose of this present study is to investigate the efficacy of vancomycin-loaded VK100 silicone cement drug delivery system in the treatment of implant-related methicillin-resistant Staphylococcus aureus (MRSA) osteomyelitis in rats. MATERIALS AND METHODS: Thirty-six adult (18-20 weeks old) female Sprague-Dawley rats were included in the study. All rats underwent experimental osteomyelitis surgery via injecting 100 µL bacterial suspension of MRSA into the medullary canal...
January 2018: Journal of Orthopaedic Surgery
David B Huang, Ian Morrissey, Timothy Murphy, Stephen Hawser, Mark H Wilcox
The objective of this study was to demonstrate the efficacy of iclaprim in a neutropenic rat lung infection model with methicillin-resistant Staphylococcus aureus (MRSA) entrapped in alginate beads. An inoculum of 5.25 × 105 colony-forming units (CFU)/mL of S. aureus strain AH1252 was administered intratracheally to rats with prepared alginate bacteria suspensions. Beginning 2 h post-infection, rats received: (1) iclaprim 80 mg/kg (n = 16); (2) iclaprim 60 mg/kg (n = 16), or (3) vancomycin 50 mg/kg (n = 24), for 3 days via subcutaneous (SC) injection every 12 h...
December 8, 2017: European Journal of Clinical Microbiology & Infectious Diseases
Tangjuan Liu, Yan Chen, Guan Bi, Jin Luo, Zhongye Du, Jinliang Kong, Yiqiang Chen
BACKGROUND Methicillin-resistant Staphylococcus aureus (MRSA) is a common pathogen responsible for many related infections, and immunosuppressed individuals are more susceptible. Its pathogenicity is associated with its virulence factors, resistance to antibiotics, and ability to form biofilm (BF). MRSA-BF infections in immunosuppressed patients pose great difficulties to clinical treatment. MATERIAL AND METHODS The study aimed to establish a model of MRSA-BF infection in rats with cyclophosphamide (CTX)-induced immunosuppression...
December 7, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Shankaramurthy Channabasappa, Murali Durgaiah, Ravisha Chikkamadaiah, Senthil Kumar, Amruta Joshi, Bharathi Sriram
Staphylococcus aureus causes systemic infections with high morbidity and mortality, and the emergence of drug-resistant strains is a rapidly growing clinical concern. Novel therapeutic agents are required to tackle S. aureus infections. P128 is a bacteriophage-derived chimeric ectolysin with potent and rapid bactericidal activity against S. aureus In the present study, the efficacy of P128 was evaluated in a newly developed rat model of S. aureus bacteremia. Prior to in vivo testing, P128 was shown to be stable in whole blood by incubation in rat blood for up to 6 h and testing its bactericidal activity against the methicillin-resistant S...
February 2018: Antimicrobial Agents and Chemotherapy
Ching-Yun Hsu, Calvin T Sung, Ibrahim A Aljuffali, Chun-Han Chen, Kai-Yin Hu, Jia-You Fang
The aim of this study was to develop PEGylated phosphatidylcholine (PC)-rich nanovesicles (phosphatiosomes) carrying ciprofloxacin (CIPX) for lung targeting to eradicate extracellular and intracellular methicillin-resistant Staphylococcus aureus (MRSA). Soyaethyl morphonium ethosulfate (SME) was intercalated in the nanovesicle surface with the dual goals of achieving strengthened bactericidal activity of CIPX-loaded phosphatiosomes and delivery to the lungs. The isothermal titration calorimetry (ITC) results proved the strong association of SME phosphatiosomes with pulmonary surfactant...
November 8, 2017: Nanomedicine: Nanotechnology, Biology, and Medicine
Ma Su, Donglin Xia, Peng Teng, Alekhya Nimmagadda, Chao Zhang, Timothy Odom, Annie Cao, Yong Hu, Jianfeng Cai
Hydantoin (imidazolidinedione) derivatives such as nitrofurantoin are small molecules that have aroused considerable interest recently due to their low rate of bacterial resistance. However, their moderate antimicrobial activity may hamper their application combating antibiotic resistance in the long run. Herein, we report the design of bacterial membrane-active hydantoin derivatives, from which we identified compounds that show much more potent antimicrobial activity than nitrofurantoin against a panel of clinically relevant Gram-positive and Gram-negative bacterial strains...
October 26, 2017: Journal of Medicinal Chemistry
R A Cetinkaya, S Yilmaz, A Ünlü, P Petrone, C Marini, E Karabulut, M Urkan, E Kaya, K Karabacak, M Uyanik, I Eker, A Kilic, A Gunal
INTRODUCTION: The wound healing properties of platelet-rich plasma (PRP) gel have been documented in many studies. PRP gel has also become a promising agent for treating surgical site infections. In this study, we investigated the antibacterial activity and wound healing effectiveness of PRP in an animal model of Methicillin-resistant Staphylococcus aureus subsp. aureus (MRSA N315)-contaminated superficial soft tissue wounds. MATERIALS AND METHODS: Subcutaneous wounds in Wistar Albino male rats were created by making two cm midline incisions followed by inoculation of microorganisms...
September 30, 2017: European Journal of Trauma and Emergency Surgery: Official Publication of the European Trauma Society
Zubin Zhou, Chenhao Pan, Ye Lu, Youshui Gao, Wei Liu, Peipei Yin, Xiaowei Yu
Osteomyelitis is commonly caused by Staphylococcus aureus. Both erythromycin and curcumin can suppress S. aureus growth, but their roles in osteomyelitis are barely studied. We aim to explore the activities of erythromycin and curcumin against chronical osteomyelitis induced by methicillin-resistant S. aureus (MRSA). Chronicle implant-induced osteomyelitis was established by MRSA infection in male Wistar rats. Four weeks after bacterial inoculation, rats received no treatment, erythromycin monotherapy, curcumin monotherapy, or erythromycin plus curcumin twice daily for 2 weeks...
2017: Frontiers in Cellular and Infection Microbiology
N F N Stoikes, J R Scott, A Badhwar, C R Deeken, G R Voeller
PURPOSE: The objective was to evaluate the host response, resorption, and strength properties, and to assess the performance in the presence of bacteria for Phasix™ Mesh (Phasix™) and Gore® Bio-A® Tissue Reinforcement (Bio-A®) in preclinical models. METHODS: In a rat model, one mesh (2 × 2 cm) was implanted subcutaneously in n = 60 rats. Animals were euthanized after 2, 4, 8, 12, 16, or 24 weeks (n = 5/mesh/time point), and implant sites were assessed for host inflammatory response and overall fibrotic repair thickness...
October 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
Sanjay Chhibber, Ashu Shukla, Sandeep Kaur
The emergence of drug resistance has rekindled interest in phage therapy as an alternative treatment option; its potency, safety, and proven efficacy are worth noting. However, phage therapy still suffers from issues of poor stability, narrow spectra, and poor pharmacokinetic profiles. Therefore, it is essential to look into the use of drug delivery systems for efficient delivery of lytic phages in vivo The present study evaluated the use of nanostructured lipid-based carriers, i.e., transfersomes, as transdermal delivery systems for encapsulating a methicillin-resistant Staphylococcus aureus (MRSA) phage cocktail...
October 2017: Antimicrobial Agents and Chemotherapy
Yan Q Xiong, Angeles Estellés, L Li, W Abdelhady, R Gonzales, Arnold S Bayer, Edgar Tenorio, Anton Leighton, Stefan Ryser, Lawrence M Kauvar
Many serious bacterial infections are antibiotic refractory due to biofilm formation. A key structural component of biofilm is extracellular DNA, which is stabilized by bacterial proteins, including those from the DNABII family. TRL1068 is a high-affinity human monoclonal antibody against a DNABII epitope conserved across both Gram-positive and Gram-negative bacterial species. In the present study, the efficacy of TRL1068 for the disruption of biofilm was demonstrated in vitro in the absence of antibiotics by scanning electron microscopy...
October 2017: Antimicrobial Agents and Chemotherapy
A Alkhalil, A Day, K W Monger, J Zhang, B C Carney, H N Hoffman, L T Moffatt, J W Shupp
OBJECTIVE: As the number of commercially available wound dressings is increasing rapidly, it is important for clinicians to understand the strengths and limitations of each and to recognise relationships between wound type and dressing properties to obtain optimal healing results. Our aim is to test the antimicrobial activity of two dressings. METHOD: A hydroconductive (HC) dressing and a silver-impregnated foam (SIF) dressing were compared for their potential to reduce the levels meticillin-resistant Staphylococcus aureus (MRSA)...
July 1, 2017: Journal of Wound Care
Oriana Simonetti, Guendalina Lucarini, Fiorenza Orlando, Elisa Pierpaoli, Roberto Ghiselli, Mauro Provinciali, Pamela Castelli, Mario Guerrieri, Roberto Di Primio, Annamaria Offidani, Andrea Giacometti, Oscar Cirioni
Prolonged hospitalization and antibiotic therapy are risk factors for the development of methicillin-resistant Staphylococcus aureus (MRSA) infections in thermal burn patients. We used a rat model to study the in vivo efficacy of daptomycin in the treatment of burn wound infections by S. aureus, and we evaluated the wound healing process through morphological and immunohistochemical analysis. A copper bar heated in boiling water was applied on a paraspinal site of each rat, resulting in two full-thickness burns...
September 2017: Antimicrobial Agents and Chemotherapy
Paulo César Venâncio, Sidney Raimundo Figueroba, Bruno Dias Nani, Luiz Eduardo Nunes Ferreira, Bruno Vilela Muniz, Fernando de Sá Del Fiol, Adilson Sartoratto, Edvaldo Antonio Ribeiro Rosa, Francisco Carlos Groppo
Purpose: This study observed the effect of garlic extracts and amoxicillin against an induced staphylococcal infection model. MIC and MBC were also obtained for aqueous extracts of Allium sativum (Asa) and Allium tuberosum (Atu) against Staphylococcus aureus penicillin-sensitive (PSSA - ATCC 25923) and MRSA (ATCC 33592). Methods: Granulation tissues were induced in the back of 205 rats. After 14 days, 0.5 mL of 10(8) CFU/mL of PSSA or MRSA were injected inside tissues. After 24h, animals were divided: G1 (Control) - 0...
April 2017: Advanced Pharmaceutical Bulletin
Mukesh K Yadav, Sung-Won Chae, Yoon Young Go, Gi Jung Im, Jae-Jun Song
Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) are known to cause biofilm-related infections. MRSA and PA have been frequently isolated from chronically infected wounds, cystic fibrosis, chronic suppurative otitis media (CSOM), and from indwelling medical devices, and these bacteria co-exist; however, their interaction with each-other or with the host is not well known. In this study, we investigated MRSA and PA multi-species biofilm communities in vitro and their interaction with the host during in vivo colonization using an OM rat-model...
2017: Frontiers in Cellular and Infection Microbiology
Jiaul Hoque, Jayanta Haldar
Cationic small molecular biocides have been developed as promising antibiofilm agents because of their tunability in chemical structures and their ability to disrupt established biofilms. However, the impact of biocides in antibiofilm treatment is largely limited due to the lack of an effective delivery system that can ensure sustained release of biocides at the target site. Herein we report a biocide-encapsulated antibacterial and antibiofilm hydrogel that acts as an efficient delivery vehicle for the biocide and eradicates matured bacterial biofilm...
May 2, 2017: ACS Applied Materials & Interfaces
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