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https://www.readbyqxmd.com/read/29238736/combination-of-fluorescent-in-situ-hybridization-fish-and-immunofluorescence-imaging-for-detection-of-cytokine-expression-in-microglia-macrophage-cells
#1
Maria Fe Lanfranco, David J Loane, Italo Mocchetti, Mark P Burns, Sonia Villapol
Microglia and macrophage cells are the primary producers of cytokines in response to neuroinflammatory processes. But these cytokines are also produced by other glial cells, endothelial cells, and neurons. It is essential to identify the cells that produce these cytokines to target their different levels of activation. We used dual RNAscope® fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) techniques to visualize the mRNA expression pattern of pro- and anti-inflammatory cytokines in microglia/macrophages cells...
November 20, 2017: Bio-protocol
https://www.readbyqxmd.com/read/29231697/health-education-assistance-loan-heal-program-final-rule
#2
(no author information available yet)
On July 1, 2014, the HEAL Program was transferred from the U.S. Department of Health and Human Services (HHS) to the U.S. Department of Education (the Department). To reflect this transfer and to facilitate the servicing of all HEAL loans that are currently held by the Department, the Secretary adds the HEAL Program regulations to the Department's chapter in the Code of Federal Regulations (CFR).
November 15, 2017: Federal Register
https://www.readbyqxmd.com/read/29231249/analysis-of-the-costs-of-veterinary-education-and-factors-associated-with-financial-stress-among-veterinary-students-in-australia
#3
K P Gregory, S M Matthew, J A Baguley
OBJECTIVE: To investigate the course-related and other costs involved in obtaining a veterinary education in Australia and how these costs are met. The study also aimed to identify sociodemographic and course-related factors associated with increased financial stress. METHODS: Students from seven Australian veterinary schools were surveyed using an online questionnaire. A total of 443 students participated (response rate 17%). Responses to survey items relating to finances, employment and course-related costs were compared with sociodemographic factors and prior research in the area of student financial stress...
December 12, 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/29230691/beta-blocker-use-in-pregnancy-and-risk-of-specific-congenital-anomalies-a-european-case-malformed-control-study
#4
Jorieke E H Bergman, L Renée Lutke, Rijk O B Gans, Marie-Claude Addor, Ingeborg Barisic, Clara Cavero-Carbonell, Ester Garne, Miriam Gatt, Kari Klungsoyr, Nathalie Lelong, Catherine Lynch, Olatz Mokoroa, Vera Nelen, Amanda J Neville, Anna Pierini, Hanitra Randrianaivo, Anke Rissmann, David Tucker, Awi Wiesel, Helen Dolk, Maria Loane, Marian K Bakker
INTRODUCTION: The prevalence of chronic hypertension is increasing in pregnant women. Beta-blockers are among the most prevalent anti-hypertensive agents used in early pregnancy. OBJECTIVE: The objective of this study was to investigate whether first-trimester use of beta-blockers increases the risk of specific congenital anomalies in offspring. METHODS: A population-based case-malformed control study was conducted in 117,122 registrations of congenital anomalies from 17 European Concerted Action on Congenital Anomalies and Twins (EUROCAT) registries participating in EUROmediCAT with data for all or part of the period between 1995 and 2013...
December 11, 2017: Drug Safety: An International Journal of Medical Toxicology and Drug Experience
https://www.readbyqxmd.com/read/29223847/funding-breakthrough-therapies-a-systematic-review-and-recommendation
#5
REVIEW
E Hanna, M Toumi, C Dussart, B Borissov, O Dabbous, K Badora, P Auquier
BACKGROUND: Advanced therapy medicinal products (ATMPs) are innovative therapies likely associated with high prices. Payers need guidance to create a balance between ensuring patient access to breakthrough therapies and maintaining the financial sustainability of the healthcare system. OBJECTIVE: The aims of this study were to identify, define, classify and compare the approaches to funding high-cost medicines proposed in the literature, to analyze their appropriateness for ATMP funding and to suggest an optimal funding model for ATMPs...
December 2, 2017: Health Policy
https://www.readbyqxmd.com/read/29205098/platelet-derived-growth-factor-receptor-alpha-expressing-cardiac-progenitor-cells-can-be-derived-from-previously-cryopreserved-human-heart-samples
#6
Thi Yen Loan Le, Hilda Pickett, Cristobal Dos Remedios, Pasquale Barbaro, Eddy Kizana, James Chong
Cardiac progenitor cells (CPCs) are being developed as a promising treatment for heart failure. Although clinical trials have predominantly used donor cardiac biopsies to derive CPCs, a better solution could be to use previously cryopreserved human heart tissue. This would enable timely and convenient access to healthy and young heart samples for CPC production. However, few studies have attempted to isolate CPCs from previously cryopreserved heart tissue. In this study, we isolated CPCs from eight non-diseased human heart samples previously cryopreserved as part of the Sydney Heart Bank...
December 5, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29187543/a-proteomic-survey-of-jun%C3%A3-n-virus-interactions-with-human-proteins-reveals-host-factors-required-for-arenavirus-replication
#7
Christopher M Ziegler, Philip Eisenhauer, Jamie A Kelly, Loan N Dang, Vedran Beganovic, Emily A Bruce, Benjamin R King, David J Shirley, Marion E Weir, Bryan A Ballif, Jason Botten
Arenaviruses are negative-strand, enveloped RNA viruses that cause significant human disease. In particular, Junín mammarenvirus (JUNV) is the etiologic agent of Argentine hemorrhagic fever. At present, little is known about the cellular proteins that the arenavirus matrix protein (Z) hijacks to accomplish its various functions, including driving the process of virus release. Further, there is a little knowledge regarding host proteins incorporated into arenavirus particles and their importance for virion function...
November 29, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29183643/selective-targeting-of-pro-inflammatory-th1-cells-by-microrna-148a-specific-antagomirs-in%C3%A2-vivo
#8
Patrick Maschmeyer, Georg Petkau, Francesco Siracusa, Jakob Zimmermann, Franziska Zügel, Anja Andrea Kühl, Katrin Lehmann, Sarah Schimmelpfennig, Melanie Weber, Claudia Haftmann, René Riedel, Markus Bardua, Gitta Anne Heinz, Cam Loan Tran, Bimba Franziska Hoyer, Falk Hiepe, Sebastian Herzog, Jürgen Wittmann, Nikolaus Rajewsky, Fritz Georg Melchers, Hyun-Dong Chang, Andreas Radbruch, Mir-Farzin Mashreghi
In T lymphocytes, expression of miR-148a is induced by T-bet and Twist1, and is specific for pro-inflammatory Th1 cells. In these cells, miR-148a inhibits the expression of the pro-apoptotic protein Bim and promotes their survival. Here we use sequence-specific cholesterol-modified oligonucleotides against miR-148a (antagomir-148a) for the selective elimination of pro-inflammatory Th1 cells in vivo. In the murine model of transfer colitis, antagomir-148a treatment reduced the number of pro-inflammatory Th1 cells in the colon of colitic mice by 50% and inhibited miR-148a expression by 71% in the remaining Th1 cells...
November 25, 2017: Journal of Autoimmunity
https://www.readbyqxmd.com/read/29141397/supporting-low-income-cancer-patients-recommendations-for-the-public-financial-aid-program-in-the-republic-of-korea
#9
Hye Sook Min, Hyung Kook Yang, Keeho Park
Purpose: As the recent term of "financial toxicity" implies, cancer causes a treatment-related financial harm. Financial Aid Program for Cancer Patient (FAPCP) is a government's financial support for low-income patients in the Republic of Korea. This study aimed to describe FAPCP applicants' condition and to investigate factors influencing financial burden, which would provide the basis for implementing a strategy for FAPCP administration. Materials and Methods: The telephone survey results from 2,700 FAPCP participants were analyzed, including demographic, socioeconomic, and disease characteristics and experiences associated with financial burden and the actions or measures to cope with them...
November 15, 2017: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29137512/microfinance-institutions-successful-delivery-of-micronutrient-powders-a-randomized-trial-in-rural-haiti
#10
Aaron Baum, Wesly Elize, Florence Jean-Louis
Globally, two-thirds of child deaths could be prevented by increased provision of health interventions such as vaccines, micronutrient supplements, and water purification tablets. We report the results from a randomized controlled trial in Haiti during 2012 that tested whether microfinance institutions-which reach 200 million households worldwide-can effectively deliver health products. These institutions provide loans to underserved entrepreneurs, primarily poor women in rural areas. In the intervention group, micronutrient powders to improve the nutrition of young children were distributed at regularly occurring microfinance meetings by a trained borrower...
November 2017: Health Affairs
https://www.readbyqxmd.com/read/29132285/empowering-caregivers-of-orphans-and-vulnerable-children-in-swaziland
#11
Syloid Choice Makufa, Daisy Kisyombe, Nicole Miller, Nanette Barkey
This paper reports on the health and psychosocial impacts of a programme designed to economically empower female caregivers of orphans and vulnerable children (OVC). The results presented are from a cohort of 492 female caregivers who participated in savings groups and were responsible for 887 OVC. The data showed that the caregivers had increased earnings, borrowed more, repaid their loans, and expanded their businesses. Important health impacts were found for both the caregivers and the OVC. Access to health care increased and the proportion of OVC and caregivers who reported being too ill to take part in daily activities decreased...
December 2017: African Journal of AIDS Research: AJAR
https://www.readbyqxmd.com/read/29121907/-helping-my-neighbour-is-like-giving-a-loan%C3%A2-the-role-of-social-relations-in-chronic-illness-in-rural-uganda
#12
Jovita Amurwon, Flora Hajdu, Dominic Bukenya Yiga, Janet Seeley
BACKGROUND: Understanding individuals' experience of accessing care and tending to various other needs during chronic illness in a rural context is important for health systems aiming to increase access to healthcare and protect poor populations from unreasonable financial hardship. This study explored the impact on households of access to free healthcare and how they managed to meet needs during chronic illness. METHODS: Rich data from the life stories of individuals from 22 households in rural south-western Uganda collected in 2009 were analysed...
November 9, 2017: BMC Health Services Research
https://www.readbyqxmd.com/read/29109563/evaluating-the-changing-financial-burdens-for-graduating-pharmacists
#13
T Joseph Mattingly, Timothy R Ulbrich
Objective. To compare new practitioners in 2009 and 2014 by modeling net income from available salary, expenditure, and student loan data. Methods. A Monte Carlo simulation with probabilistic sensitivity analysis was conducted to model net income for graduating pharmacists in 2009 and 2014. Mean and standard deviations were recorded for each model parameter. Student t-tests were used to compare the mean differences between 2009 and 2014 cohorts. Results. Pharmacist salary and disposable income were higher on average in 2014 compared with 2009...
September 2017: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/29109558/associations-between-pharmacy-students-attitudes-toward-debt-stress-and-student-loans
#14
Marie A Chisholm-Burns, Christina A Spivey, Melanie C Jaeger, Jennifer Williams
Objective. To assess graduating pharmacy students' attitudes toward debt and determine associations with stress, student loan debt, financial need, current employment, post-graduation plans, and expected length of time to repay loans. Methods. Survey was conducted using an attitudes-toward-debt scale (sub-scales: tolerant attitudes toward debt; contemplation and knowledge about loans; fear of debt), Perceived Stress Scale, and questions concerning current employment, estimated total student loan debt, post-graduation plans, and expected length of time to repay loans...
September 2017: American Journal of Pharmaceutical Education
https://www.readbyqxmd.com/read/29082547/-11-c-pe2i-and-18-f-dopa-pet-for-assessing-progression-rate-in-parkinson-s-a-longitudinal-study
#15
Weihua Li, Nick P Lao-Kaim, Andreas A Roussakis, Antonio Martín-Bastida, Natalie Valle-Guzman, Gesine Paul, Clare Loane, Håkan Widner, Marios Politis, Tom Foltynie, Roger A Barker, Paola Piccini
BACKGROUND: (18) F-dopa PET measuring aromatic l-amino acid decarboxylase activity is regarded as the gold standard for evaluating dopaminergic function in Parkinson's disease. Radioligands for dopamine transporters are also used in clinical trials and for confirming PD diagnosis. Currently, it is not clear which imaging marker is more reliable for assessing clinical severity and rate of progression. The objective of this study was to directly compare (18) F-dopa with the highly selective dopamine transporter radioligand (11) C-PE2I for the assessment of motor severity and rate of progression in PD...
October 30, 2017: Movement Disorders: Official Journal of the Movement Disorder Society
https://www.readbyqxmd.com/read/29074930/does-student-debt-affect-dental-students-and-dentists-stress-levels
#16
J D Boyles, B Ahmed
Introduction Many studies have shown financial worries and debt to induce stress in individuals, this combined with the existing stress of being a dentist raises the question of how student debt affects students' and dentists' stress levels.Objectives Determine whether student debt has had any noticeable effect on student stress levels; investigate whether student debt has any effect on dentists' career choice; investigate whether the increase in tuition fees has influenced the number of applicants to study dentistry at the University of Birmingham...
October 27, 2017: British Dental Journal
https://www.readbyqxmd.com/read/29070157/academic-pediatric-dentistry-is-a-rewarding-financially-viable-career-path
#17
Janice A Townsend, Donald L Chi
Newly graduated pediatric dentists have unprecedented levels of debt. High levels of student debt may be perceived as an obstacle to pursue an academic career. However, opportunities exist through faculty compensation models and loan repayment programs that make an academic career financially viable. The purpose of this paper is to outline the benefits of a career in academic dentistry and provide examples of young pediatric dentistry faculty members who have been able to manage student debt while pursuing meaningful and rewarding careers...
September 15, 2017: Pediatric Dentistry
https://www.readbyqxmd.com/read/29069451/poor-recovery-of-households-from-out-of-pocket-payment-for-assisted-reproductive-technology
#18
Silke J Dyer, Latiefa Vinoos, John E Ataguba
STUDY QUESTION: How do households recover financially from direct out-of-pocket payment for government subsidized ART? SUMMARY ANSWER: After a mean of 3.8 years, there was poor recovery from initiated financial coping strategies with the poorest households being disproportionatley affected. WHAT IS KNOWN ALREADY: Out-of-pocket payment for health services can create financial burdens for households and inequities in access to care. A previous study conducted at a public-academic institution in South Africa documented that patient co-payment for one cycle of ART resulted in catastrophic expenditure for one in five households, and more frequently among the poorest, requiring diverse financial coping strategies to offset costs...
October 21, 2017: Human Reproduction
https://www.readbyqxmd.com/read/29057371/dysflective-cones-visual-function-and-cone-reflectivity-in-long-term-follow-up-of-acute-bilateral-foveolitis
#19
Joanna H Tu, Katharina G Foote, Brandon J Lujan, Kavitha Ratnam, Jia Qin, Michael B Gorin, Emmett T Cunningham, William S Tuten, Jacque L Duncan, Austin Roorda
PURPOSE: Confocal adaptive optics scanning laser ophthalmoscope (AOSLO) images provide a sensitive measure of cone structure. However, the relationship between structural findings of diminished cone reflectivity and visual function is unclear. We used fundus-referenced testing to evaluate visual function in regions of apparent cone loss identified using confocal AOSLO images. METHODS: A patient diagnosed with acute bilateral foveolitis had spectral-domain optical coherence tomography (SD-OCT) (Spectralis HRA + OCT system [Heidelberg Engineering, Vista, CA, USA]) images indicating focal loss of the inner segment-outer segment junction band with an intact, but hyper-reflective, external limiting membrane...
September 2017: American Journal of Ophthalmology Case Reports
https://www.readbyqxmd.com/read/29051721/a-comparison-of-medical-education-in-germany-and-the-united-states-from-applying-to-medical-school-to-the-beginnings-of-residency
#20
REVIEW
Dmitry Zavlin, Kevin T Jubbal, Jonas G Noé, Bernd Gansbacher
Both Germany and the United States of America have a long tradition of science and medical excellence reaching back as far as the nineteenth century. The same tribute must be paid to the medical educational system in both countries. Despite significant initial similarities and cross-inspiration, the paths from enrolling in a medical university to graduating as a medical doctor in Germany and the US seem to have become much different. To fill a void in literature, the authors' objective therefore is to delineate both structures of medical education in an up-to-date review and examine their current differences and similarities...
2017: German Medical Science: GMS E-journal
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