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https://www.readbyqxmd.com/read/18320355/apoptosis-in-fresh-and-cryopreserved-cardiac-valves-of-pig-samples
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M Esther Rendal Vázquez, T M Díaz Román, M Rodríguez Cabarcos, C Zavanella Botta, N Domenech García, M González Cuesta, M J Sánchez Dopico, S Pértega Díaz, C Andión Núñez
To analyse the influence of cold ischemic time (CIT) (2-24 h) and of cryopreservation (liquid phase) on the viability of the valvular fibroblasts and in the presence of apoptosis. Cardiac valves from 10 pigs were evaluated by anatomo-pathological study of the wall, muscle and leaflet. At the same time, the presence of cellular death due to apoptosis was investigated in two ways; directly on tissue by Apodetec system and by two-colour flow cytometry assay analyzing a suspension of fibroblast from valve leaflets using Anexina V and propidium iodure (PI)...
June 2008: Cell and Tissue Banking
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