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https://www.readbyqxmd.com/read/28213128/quantitative-proteomic-analysis-of-human-testis-reveals-system-wide-molecular-and-cellular-pathways-associated-with-non-obstructive-azoospermia
#1
Mehdi Alikhani, Mehdi Mirzaei, Marjan Sabbaghian, Pouria Parsamatin, Razieh Karamzadeh, Samane Adib, Niloofar Sodeifi, Mohammad Ali Sadighi Gilani, Masoud Zabet-Moghaddam, Lindsay Parker, Yunqi Wo, Vivek Gupta, Paul A Haynes, Hamid Gourabi, Hossein Baharvand, Ghasem Hosseini Salekdeh
: Male infertility accounts for half of the infertility problems experienced by couples. Azoospermia, having no measurable level of sperm in seminal fluid, is one of the known conditions resulting in male infertility. In order to elucidate the complex molecular mechanisms causing male azoospermia, label-free quantitative shotgun proteomics was carried out on testicular tissue specimens from patients with obstructive azoospermia and non-obstructive azoospermia, including maturation arrest (MA) and Sertoli cell only syndrome (SCOS)...
February 14, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28190904/which-early-works-are-cited-most-frequently-in-climate-change-research-literature-a-bibliometric-approach-based-on-reference-publication-year-spectroscopy
#2
Werner Marx, Robin Haunschild, Andreas Thor, Lutz Bornmann
This bibliometric analysis focuses on the general history of climate change research and, more specifically, on the discovery of the greenhouse effect. First, the Reference Publication Year Spectroscopy (RPYS) is applied to a large publication set on climate change of 222,060 papers published between 1980 and 2014. The references cited therein were extracted and analyzed with regard to publications, which are cited most frequently. Second, a new method for establishing a more subject-specific publication set for applying RPYS (based on the co-citations of a marker reference) is proposed (RPYS-CO)...
2017: Scientometrics
https://www.readbyqxmd.com/read/28187743/the-influence-of-macro-and-microelements-in-seminal-plasma-on-diluted-boar-sperm-quality
#3
Maja Zakošek Pipan, Janko Mrkun, Breda Jakovac Strajn, Katarina Pavšič Vrtač, Janko Kos, Anja Pišlar, Petra Zrimšek
BACKGROUND: Growing evidence indicates that macro- and microelements in the seminal plasma of humans and various domestic animals are of great importance due to their roles in sperm metabolism, function, survival and oxidative stress. In the present study, we therefore determined the concentrations of macro- and microelements in fresh boar seminal plasma and their relation to sperm quality parameters after 3 days of liquid storage was assessed. Twenty ejaculates from eight boars were collected, and semen volume, concentration, sperm motility, morphology, tail membrane integrity, plasma membrane permeability, mitochondrial membrane potential and DNA fragmentation were determined on the day of collection (day 0) and day 3 (72 h) of storage at 15-17 °C...
February 10, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28174513/cervical-mucus-proteome-in-endometriosis
#4
Giuseppe Grande, Federica Vincenzoni, Domenico Milardi, Giuseppina Pompa, Domenico Ricciardi, Erika Fruscella, Francesca Mancini, Alfredo Pontecorvi, Massimo Castagnola, Riccardo Marana
BACKGROUND: Endometriosis is a chronic gynecological inflammatory disease characterized by the presence of functional endometrial glands and stroma outside of the uterine cavity. It affects 7-10% of women of reproductive age and up to 50% of women with infertility. The current gold standard for the diagnosis combines laparoscopic evaluation and biopsy of the visualized lesions. However, laparoscopy requires general anesthesia and developed surgical skills and it has a high procedural cost...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28166982/dietary-polyunsaturated-fatty-acid-supplementation-of-young-post-pubertal-dairy-bulls-alters-the-fatty-acid-composition-of-seminal-plasma-and-spermatozoa-but-has-no-effect-on-semen-volume-or-sperm-quality
#5
C J Byrne, S Fair, A M English, S A Holden, J R Dick, P Lonergan, D A Kenny
The aim of this study was to examine the effects of dietary supplementation with rumen protected n-6 or n-3 polyunsaturated fatty acids (PUFA) on the quantity and quality of semen from young post-pubertal dairy bulls. Pubertal Holstein-Friesian (n = 43) and Jersey (n = 7) bulls with a mean ± s.e.m. age and bodyweight of 420.1 ± 5.86 days and 382 ± 8.94 kg, respectively, were blocked on breed, weight, age and semen quality (based on the outcomes of two pre-trial ejaculates) and randomly assigned to one of three treatments: (i) a non-supplemented control (CTL, n = 15), (ii) rumen-protected safflower (SO, n = 15), (iii) rumen-protected n-3 PUFA-enriched fish oil (FO, n = 20)...
March 1, 2017: Theriogenology
https://www.readbyqxmd.com/read/28164613/high-concentrations-of-the-angiogenic-peptide-vegf-a-in-seminal-fluid-and-its-association-to-prostasomes
#6
Lena Carlsson, Gunnar Ronquist, Rune Elisasson, Louise Dubois, Karl G Ronquist, Anders Larsson
BACKGROUND: Angiogenesis is the formation of new blood vessels by capillary sprouting from pre-existing vessels. This process is associated with increased expression of angiogenic factors like vascular endothelial growth factor (VEGF). The VEGF family consists of five members denoted VEGF-A, B, C, D and placenta growth factor (PlGF). Prostasomes are exosome-like extracellular vesicles existing in seminal plasma. The present study aimed at investigating the possible relationship between VEGF-A in seminal fluid and blood plasma and the prostasomal association of VEGF-A...
August 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28164351/ethanol-induced-male-infertility-effects-of-aqueous-leaf-extract-of-tetracarpidium-conophorum
#7
S F Akomolafe, G Oboh, A A Akindahunsi, A J Afolayan
This study investigated the effects of Tetracarpidium conophorum leaf extract on infertility induced by ethanol in male rats. Thirty rats were randomly divided into six groups of five animals each: Group 1 (positive control) received 0.9% saline only; Group 2 (ethanol alone) were given only 30% ethanol orally at 7 ml/kg body weight per day, thrice in a week; groups 3, 4 and 5 were given ethanol and co-treated with 50, 500 and 1,000 mg/kg body weight of leaf extract, respectively, while Group 6 was given ethanol and co-treated with a fertility drug, clomiphene citrate...
February 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28161861/aryl-hydrocarbon-receptor-ahr-rs2066853-gene-polymorphism-association-with-infertile-oligoasthenoteratozoospermic-men-and-seminal-oxidative-stress
#8
Taymour Mostafa, Hanan Fouad, Nashaat Nabil, Laila Rashed, Dina Sabry, Khadiga Abougabal, Bolis S Gendy
This study aimed to assess the association between aryl hydrocarbon receptor (AhR) rs2066853 gene polymorphism with infertile oligoasthenoteratozoospermic (OAT) men and seminal oxidative stress (OS). A total of 170 Egyptian men were allocated according to their semen analysis into fertile normozoospermic controls (n = 50) and infertile OAT men (n = 120). They were subjected to history taking, clinical examination, semen analysis, estimation of seminal glutathione peroxidase (GPx), and malondialdehyde (MDA)...
February 4, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28159940/the-impact-of-bmi-on-sperm-parameters-and-the-metabolite-changes-of-seminal-plasma-concomitantly
#9
Dan Guo, Wei Wu, Qiuqin Tang, Shanlei Qiao, Yiqiu Chen, Minjian Chen, Mengying Teng, Chuncheng Lu, Hongjuan Ding, Yankai Xia, Lingqing Hu, Daozhen Chen, Jiahao Sha, Xinru Wang
The development of male infertility increased rapidly worldwide, which coinciding with the epidemic of obesity. However, the impact of weight abnormalities on sperm quality is still contestable. To assess the correlation between BMI and sperm parameters, we searched relevant articles in PubMed, Embase, Web of science, and Wanfang database published until June 2015 without language restriction. Otherwise, we also recruited some participants who attended fertility clinic as well as some general populations in this report...
January 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/28146677/between-two-worlds
#10
Peter A Hancock, Carryl L Baldwin, Joel S Warm, James L Szalma
OBJECTIVE: To provide an evaluative overview of the life and contributions of Raja Parasuraman. BACKGROUND: From his earliest contributions in clarifying and explaining the problematic area of vigilance to his most recent interdisciplinary advances in understanding how genotype relates to behavior in complex technical environments, Raja Parasuraman was a giant of human factors and ergonomics. Our present exposition articulates and recounts his many contributions to our science and to science in general beyond the confines of our own discipline...
February 2017: Human Factors
https://www.readbyqxmd.com/read/28145637/in-silico-identification-of-proteins-associated-with-drug-induced-liver-injury-based-on-the-prediction-of-drug-target-interactions
#11
Sergey Ivanov, Maxim Semin, Alexey Lagunin, Dmitry Filimonov, Vladimir Poroikov
Drug-induced liver injury (DILI) is the leading cause of acute liver failure as well as one of the major reasons for drug withdrawal from clinical trials and the market. Elucidation of molecular interactions associated with DILI may help to detect potentially hazardous pharmacological agents at the early stages of drug development. The purpose of our study is to investigate which interactions with specific human protein targets may cause DILI. Prediction of interactions with 1534 human proteins was performed for the dataset with information about 699 drugs, which were divided into three categories of DILI: severe (178 drugs), moderate (310 drugs) and without DILI (211 drugs)...
February 1, 2017: Molecular Informatics
https://www.readbyqxmd.com/read/28145063/a-closer-look-at-novel-climates-new-methods-and-insights-at-continental-to-landscape-scales
#12
Colin R Mahony, Alex J Cannon, Tongli Wang, Sally N Aitken
Novel climates-emerging conditions with no analog in the observational record-are an open problem in ecological modelling. Detecting extrapolation into novel conditions is a critical step in evaluating bioclimatic projections of how species and ecosystems will respond to climate change. However, biologically-informed novelty detection methods remain elusive for many modeling algorithms. To assist with bioclimatic model design and evaluation, we present a first-approximation assessment of general novelty based on a simple and consistent characterization of climate...
February 1, 2017: Global Change Biology
https://www.readbyqxmd.com/read/28138186/relationship-of-seminal-free-l-carnitine-with-functional-spermatozoal-characteristics-results-from-an-observational-study-conducted-in-a-tertiary-care-hospital-of-karachi-pakistan
#13
Syed Danish Haseen Ahmed, Shahid Ahsan, Tehseen Iqbal, Syed Intesar Ahmed Burney
OBJECTIVE: To investigate the relationship of seminal free L-carnitine with functional spermatozoal characteristics. METHODS: This observational study was conducted at the Jinnah Postgraduate Medical Centre, Karachi, from August 2009 to June 2013, and comprised fertile and infertile subjects. Semen analysis was performed and reported for its volume, sperm count, motility and morphology according to the World Health Organisation's guidelines. Seminal free L-carnitine was estimated by high-performance liquid chromatography...
February 2017: JPMA. the Journal of the Pakistan Medical Association
https://www.readbyqxmd.com/read/28137417/effect-of-age-on-biochemical-recurrence-after-radical-prostatectomy
#14
Cuneyt Ozden, Binhan Kagan Aktas, Suleyman Bulut, Guven Erbay, Suleyman Tagci, Cevdet S Gokkaya, Mehmet M Baykam, Ali Memis
The aim of the study was to evaluate the relationship between patient's age and biochemical recurrence (BCR) after radical retropubic prostatectomy (RRP). Data from RRP applied to 305 patients with clinically localized prostate cancer were included in the study. Patients were divided into the three age groups, < 60 years, 60-70 years, and > 70 years. The groups were compared regarding adverse pathological findings on RRP specimen, BCR, and biochemical recurrence-free survival (bRFS) rates. The rates of positive surgical margin, seminal vesicle invasion, lymph node involvement, RRP specimens' Gleason score, and BCR were not significantly different among the three age groups...
February 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28130393/irradiation-affects-germ-and-somatic-cells-in-prepubertal-monkey-testis-xenografts
#15
I Tröndle, B Westernströer, J Wistuba, N Terwort, S Schlatt, N Neuhaus
STUDY QUESTION: Does irradiation evoke adverse effects in germ and somatic cells in testis xenografts from prepubertal monkeys? SUMMARY ANSWER: In addition to the expected depletion of germ cells, a dose-dependent effect of irradiation was observed at the mRNA and protein level in Sertoli and peritubular myoid cells. WHAT IS KNOWN ALREADY: Testicular irradiation studies in monkeys have focused on the dose-dependent effects on germ cells. Previous studies using intact animals or xenografts reported that germ cells are highly sensitive to irradiation...
January 27, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28128902/step-by-step-laparoscopic-vesiculectomy-for-hemospermia
#16
Marcos Figueiredo Mello, Hiury Silva Andrade, Victor Srougi, Marco Antonio Arap, Anuar Ibrahim Mitre, Ricardo Jordão Duarte, Miguel Srougi
: Hemospermia has been considered as a benign and self-limiting condition. It usually has an inflammatory or infectious cause. However, recurrent or persistent hemospermia may indicate a more serious underlying pathology, especially over 40 years of age. Biopsy or surgical excision is indicated in cases of suspicious findings during investigation, such as cysts or masses. Open surgery has been considered the definitive form of treatment, however, it can be associated with significant morbidity...
January 27, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28125986/clinical-analysis-of-small-cell-carcinoma-of-the-bladder-in-chinese-nine-case-reports-and-literature-reviews
#17
Zhi Chen, Qingquan Liu, Ruibao Chen, Zhuo Liu, Mingchao Li, Qing Ling, Licheng Wu, Jun Yang, Xiaming Liu, Tao Wang, Zhiquan Hu, Xiaoling Guo, Shaogang Wang, Weiming Yang, Jihong Liu
BACKGROUND: Small cell carcinoma of the bladder (SCCB) is a kind of rare and highly aggressive tumor that is present in an advanced stage and has a propensity for early metastasis. The main presenting symptom of SCCB is hematuria. Surgery, chemotherapy, and radiotherapy, either alone or as a part of combined therapy, have been used as the treatment. The aim of this study is to present our experience with 9 SCCB patients who were treated with different modalities and to share the findings upon reviewing the literatures for patients with SCCB reported in 56 literatures in Chinese...
January 26, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28125168/prednisone-treatment-in-infertile-patients-with-oligozoospermia-and-accessory-gland-inflammatory-alterations
#18
D Milardi, G Luca, G Grande, M Ghezzi, N Caretta, G Brusco, G De Filpo, R Marana, A Pontecorvi, R Calafiore, C Foresta, A Garolla
The association between inflammation of the male reproductive system and oligozoospermia has been frequently reported in the clinical work-up of male infertility. To improve sperm parameters in infertile patients with genital inflammation, many phytochemical and nutraceutical drugs are currently being used. However, their use is still empirical and no conclusive data have been provided about their efficacy. The treatment with steroid anti-inflammatory drugs might be useful in reducing inflammation and improving sperm parameters, thus increasing the fertility outcome...
January 26, 2017: Andrology
https://www.readbyqxmd.com/read/28124472/metabonomic-analysis-of-fatty-acids-in-seminal-plasma-between-healthy-and-asthenozoospermic-men-based-on-gas-chromatography-mass-spectrometry
#19
B Tang, X Shang, H Qi, J Li, B Ma, G An, Q Zhang
The mechanism of asthenozoospermia remains unclear. The knowledge of the metabolism of fatty acids in seminal plasma is important and meaningful for the pathological study of asthenozoospermia. We present an optimised assay of extraction and derivatisation followed by GC/MS to analyse metabolites, especially fatty acids, in seminal plasma from healthy and asthenozoospermic men. Eighty-nine peaks including 17 kinds of fatty acids were analysed and identified in the chromatogram. The GC/MS data were analysed using t test, fold change and partial least squares discriminant analysis to explore the potential biomarkers of asthenozoospermia...
January 26, 2017: Andrologia
https://www.readbyqxmd.com/read/28123480/correlative-study-on-the-jak-stat-psm%C3%AE-3-signal-transduction-pathway-in-asthenozoospermia
#20
Junguo Li, Li Zhang, Bing Li
The aim of the present study was to investigate the possible mechanism of Janus kinase (JAK)-signal transduction and activator of transcription (STAT)/PSMβ3 signaling in the occurrence of asthenozoospermia. We examined seminal fluid samples from 30 cases of asthenozoospermia and 30 healthy controls. Sperm was collected using the Percoll density gradient centrifugation method. The expression of JAK, STAT and PSMβ3 mRNA was assessed by reverse-transcription quantitative PCR and the protein levels of p-JAK, p-STAT and PSMβ3 were measured by western blot analysis...
January 2017: Experimental and Therapeutic Medicine
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