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https://www.readbyqxmd.com/read/29223503/multiple-collections-of-common-carp-cyprinus-carpio-l-semen-during-the-reproductive-period-and-its-effects-on-sperm-quality
#1
Beata Irena Cejko, Beata Sarosiek, Sławomir Krejszeff, Radosław Kajetan Kowalski
Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage. Semen quantity (semen volume and sperm count), quality (sperm motility and sperm viability), as well as seminal plasma parameters (pH of seminal plasma and seminal plasma osmotic pressure) and its enzymatic activity, e...
December 5, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29223009/influence-of-spermatozoal-lipidomic-profile-on-the-cryoresistance-of-frozen-spermatozoa-from-stallions
#2
T Cabrera, C Ramires-Neto, K R A Belaz, C P Freitas-Dell'aqua, D Zampieri, A Tata, M N Eberlin, M A Alvarenga, F F Souza
The membrane of spermatozoa, which contributes to cellular cryoresistance, contains numerous lipids with a composition that directly affects membrane fluidity and the fertilization process. In light of variations in the degree of sensitivity in equine seminal freezing, this study aimed to correlate equine semen lipids with post-thawing characteristics of spermatozoa. We used ejaculates from 34 stallions, which were evaluated (total motility ≥ 60%), frozen and thawed and reevaluated for motility of spermatozoa, membrane integrity and lipid peroxidation...
November 26, 2017: Theriogenology
https://www.readbyqxmd.com/read/29222995/updating-edwin-chadwick-s-seminal-work-on-geographical-inequalities-by-occupation
#3
Mark A Green, Danny Dorling, Richard Mitchell
To honour the 175th anniversary of Edwin Chadwick's seminal 'Report on the Sanitary Conditions of the Labouring Poor', we update Chadwick's famous analysis of geographical differences in occupational based inequalities. Much of the field of Health Geography owes both its direction of development and its initial impetus to his 1842 report. The report presented evidence for the importance of local context to health, with individuals of the lowest occupations in Rutland living longer than individuals of the highest occupations in Liverpool...
December 1, 2017: Social Science & Medicine
https://www.readbyqxmd.com/read/29221541/the-global-health-nursing-imperative-using-competency-based-analysis-to-strengthen-accountability-for-population-focused-practice-education-and-research
#4
Barbara Joyce, Nancy Brown-Schott, Vicki Hicks, Regina Gina Johnson, Monica Harmon, Lucille Pilling
BACKGROUND: The Quad Council Competencies for Public Health Nurses are used to guide community and public health nursing course development in baccalaureate nursing programs. As clinical practice has expanded to global settings, the appropriateness of the 2011 Quad Council Public Health Nursing (PHN) Competencies to guide global clinical practice and evaluation was questioned. OBJECTIVE: To describe a comparison and analysis of three sets of competencies: PHN competencies, competencies for global health nurses, and interprofessional competencies for health professionals...
May 2017: Annals of Global Health
https://www.readbyqxmd.com/read/29211922/local-blood-flow-in-peripheral-nerves-and-their-ganglia-resurrecting-key-ideas-around-its-measurement-and-significance
#5
REVIEW
Douglas W Zochodne
Over three decades ago, seminal work by Phillip Low and colleagues established exquisite physiology around the measurement of nerve blood flow (NBF). Although not widely explored recently, its connection to the clinic has awaited human methodology. While human studies have not achieved a convincing level of rigour, newer imaging technologies are offering early information. The peripheral nerve trunk has parallel blood flow compartments that include epineurial flow dominated by arteriovenous shunts and downstream endoneurial blood flow (EBF)...
December 6, 2017: Muscle & Nerve
https://www.readbyqxmd.com/read/29188202/seminal-plasma-enables-selection-and-monitoring-of-active-surveillance-candidates-using-nuclear-magnetic-resonance-based-metabolomics-a-preliminary-investigation
#6
Matthew J Roberts, Renee S Richards, Clement W K Chow, Marion Buck, John Yaxley, Martin F Lavin, Horst Joachim Schirra, Robert A Gardiner
Background: Diagnosis and monitoring of localized prostate cancer requires discovery and validation of noninvasive biomarkers. Nuclear magnetic resonance (NMR)-based metabolomics of seminal plasma reportedly improves diagnostic accuracy, but requires validation in a high-risk clinical cohort. Materials and methods: Seminal plasma samples of 151 men being investigated for prostate cancer were analyzed with 1H-NMR spectroscopy. After adjustment for buffer (add-to-subtract) and endogenous enzyme influence on metabolites, metabolite profiling was performed with multivariate statistical analysis (principal components analysis, partial least squares) and targeted quantitation...
December 2017: Prostate International
https://www.readbyqxmd.com/read/29186618/semi-automatized-segmentation-method-using-image-based-flow-cytometry-to-study-sperm-physiology-the-case-of-capacitation-induced-tyrosine-phosphorylation
#7
Arturo Matamoros-Volante, Ayelen Moreno-Irusta, Paulina Torres-Rodriguez, Laura Giojalas, María G Gervasi, Pablo E Visconti, Claudia L Treviño
STUDY QUESTION: Is image-based flow cytometry a useful tool to study intracellular events in human sperm such as protein tyrosine phosphorylation or signaling processes? SUMMARY ANSWER: Image-based flow cytometry is a powerful tool to study intracellular events in a relevant number of sperm cells, which enables a robust statistical analysis providing spatial resolution in terms of the specific subcellular localization of the labeling. WHAT IS KNOWN ALREADY: Sperm capacitation is required for fertilization...
November 25, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29175285/development-of-hybeacon%C3%A2-probes-for-specific-mrna-detection-using-body-fluids-as-a-model-system
#8
Beccy Stafford-Allen, Nick Dawnay, Erin K Hanson, Glyn Ball, Ambika Gupta, Stephen Blackman, David J French, Nicola Duxbury, Jack Ballantyne, Simon Wells
HyBeacons are linear oligonucleotides which incorporate fluorescent dyes covalently linked to internal nucleotides. They have previously been used with PCR and isothermal amplification to interrogate SNPs and STRs in fields as diverse as clinical diagnostics, food authentication, and forensic DNA profiling. This work explores their use for the identification of expressed gene sequences through mRNA profiling. The use of mRNA is becoming increasingly common in forensic casework to identify body fluids on evidence items, as it offers higher specificity and fewer false positives than current chemical presumptive testing methods...
November 22, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/29160237/chemical-vapor-deposition-growth-of-boron-carbon-nitrogen-layers-from-methylamine-borane-thermolysis-products
#9
Fabrice Leardini, Eduardo Flores, Andres R Galvis, Isabel Jimenez-Ferrer, Jose-Ramon Ares, Carlos Sanchez, Pablo Molina, Herko P van der Meulen, Cristina Gomez-Navarro, Guillermo Lopez-Polin, Fernando J Urbanos, Daniel Granados, F Javier Garcia-Garcia, Umit B Demirci, Pascal G Yot, Filippo Mastrangelo, Maria Grazia Betti, Carlo Mariani
This work investigates the growth of B-C-N layers by Chemical Vapor Deposition using methylamine borane (MeAB) as single-source precursor. MeAB has been synthesized and characterized, paying particular attention to the analysis of its thermolysis products, which are the gaseous precursors for B-C-N growth. Samples have been grown on Cu foils and transferred onto different substrates for their morphological, structural, chemical, electronic and optical characterizations. The results of these characterizations indicate a segregation of h-BN and Graphene-like (Gr) domains...
November 21, 2017: Nanotechnology
https://www.readbyqxmd.com/read/29156362/test-anxiety-effects-predictors-and-correlates-a-30-year-meta-analytic-review
#10
REVIEW
Nathaniel von der Embse, Dane Jester, Devlina Roy, James Post
BACKGROUND AND OBJECTIVES: Since the 1988 publication of Hembree's seminal meta-analysis on test anxiety, much has changed in the theoretical conceptualization of test anxiety, as well as the influences on test performance. The present study examined the influence of test anxiety on a variety of educational outcomes, in addition to demographic and intrapersonal correlates. DESIGN AND METHODS: Results of 238 studies from 1988 to the present, were synthesized via a meta-analytic framework to explicate predictors, correlates, and relationships with the test anxiety construct...
November 13, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/29155178/induction-of-immune-related-gene-expression-by-seminal-exosomes-in-the-porcine-endometrium
#11
Rulan Bai, Zeinab Latifi, Kazuya Kusama, Keigo Nakamura, Masayuki Shimada, Kazuhiko Imakawa
Seminal plasma (SP) is considered as a vehicle to carry sperm into female reproductive tract, of which functions have not been completely understood. This study aimed to identify the function of seminal exosomes on porcine endometrium. Exosomes were isolated from the sperm-rich fraction of boar semen and were confirmed by the expression of exosome marker HSP70 and size distribution using nano-sight tracking analysis. Porcine endometrial epithelial cells (EECs) were then treated with seminal exosomes, and RNA extracted were subjected to global expression analysis...
November 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29153300/cognitive-dissonance-and-pediatric-procedural-pain-management-a-concept-clarification
#12
April A Bice
Pediatric nurses have often reported that pain management is a vital part of patient care. Evidence, however, suggests pediatric procedural pain treatments are often underused. Cognitive dissonance, the mental conflict leading to unpleasant thoughts and or feelings, may be related to this evidence-based gap found between what pediatric nurses claim about procedural pain management (that it is important) and what they actually do (underutilize pain treatments). The purpose of this manuscript is to clarify and further develop the concept of cognitive dissonance in terms of its relationship to nurses' mental struggles with underutilization of pediatric procedural pain treatments...
November 15, 2017: Pain Management Nursing: Official Journal of the American Society of Pain Management Nurses
https://www.readbyqxmd.com/read/29145710/testosterone-replacement-in-transgenic-sickle-cell-mice-controls-priapic-activity-and-upregulates-pde5-expression-and-enos-activity-in-the-penis
#13
B Musicki, S Karakus, W Akakpo, F H Silva, J Liu, H Chen, B R Zirkin, A L Burnett
Sickle cell disease (SCD)-associated priapism is characterized by decreased nitric oxide (NO) signaling and downregulated phosphodiesterase (PDE)5 protein expression and activity in the penis. Priapism is also associated with testosterone deficiency, but molecular mechanisms underlying testosterone effects in the penis in SCD are not known. Given the critical role of androgens in erection physiology and NO synthase (NOS)/PDE5 expression, we hypothesized that testosterone replacement to eugonadal testosterone levels reduces priapism by reversing impaired endothelial (e)NOS activity and molecular abnormalities involving PDE5...
November 16, 2017: Andrology
https://www.readbyqxmd.com/read/29132323/integrative-microrna-and-mrna-deep-sequencing-expression-profiling-in-endemic-burkitt-lymphoma
#14
Cliff I Oduor, Yasin Kaymaz, Kiprotich Chelimo, Juliana A Otieno, John Michael Ong'echa, Ann M Moormann, Jeffrey A Bailey
BACKGROUND: Burkitt lymphoma (BL) is characterized by overexpression of the c-myc oncogene, which in the vast majority of cases is a consequence of an IGH/MYC translocation. While myc is the seminal event, BL is a complex amalgam of genetic and epigenetic changes causing dysregulation of both coding and non-coding transcripts. Emerging evidence suggest that abnormal modulation of mRNA transcription via miRNAs might be a significant factor in lymphomagenesis. However, the alterations in these miRNAs and their correlations to their putative mRNA targets have not been extensively studied relative to normal germinal center (GC) B cells...
November 13, 2017: BMC Cancer
https://www.readbyqxmd.com/read/29124782/the-effect-of-cigarette-smoking-on-human-seminal-parameters-sperm-chromatin-structure-and-condensation
#15
R M Mostafa, Y S Nasrallah, M M Hassan, A F Farrag, A Majzoub, A Agarwal
Considerable debate still exists regarding the effects of cigarette smoking on male fertility. This work aimed to explore effects of cigarette smoking on semen parameters and DNA fragmentation on 95 infertile patients who were divided into infertile male nonsmokers (45) and infertile male smokers (50). Smokers were subdivided according to a number of cigarettes smoked per day into mild (≤10), moderate (11-20) and heavy smokers (≥21). Semen analysis, sperm chromatin condensation integrity with aniline blue staining and sperm viability were compared between the study groups...
November 9, 2017: Andrologia
https://www.readbyqxmd.com/read/29121862/human-papillomavirus-in-semen-and-the-risk-for-male-infertility-a-systematic-review-and-meta-analysis
#16
Zhangyan Lyu, Xiaoshuang Feng, Ni Li, Wei Zhao, Luopei Wei, Yuheng Chen, Wenjing Yang, Hongxia Ma, Bing Yao, Kai Zhang, Zhibin Hu, Hongbing Shen, Dong Hang, Min Dai
BACKGROUND: Human papillomavirus (HPV) is one of the most prevalent sexually transmitted viruses. Despite the increasing evidence of HPV prevalence in semen, the worldwide distribution of HPV types in semen and risk for male infertility remain inconclusive. METHODS: Four electronic databases were searched for English language studies conducted between January 1990 and December 2016 that reported HPV DNA prevalence in semen. Based on the PRISMA guidelines, HPV prevalence was estimated among general population and fertility clinic attendees, respectively, and heterogeneity testing was performed using Cochran's Q and I (2) statistics...
November 9, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/29116490/impact-of-sarcopenic-obesity-on-failure-to-rescue-from-major-complications-following-pancreaticoduodenectomy-for-cancer-results-from-a-multicenter-study
#17
Nicolò Pecorelli, Giovanni Capretti, Marta Sandini, Anna Damascelli, Giulia Cristel, Francesco De Cobelli, Luca Gianotti, Alessandro Zerbi, Marco Braga
BACKGROUND: Failure to rescue (FTR) is a quality-of-care indicator in pancreatic surgery, but may also identify patients who may not tolerate major postoperative complications despite being treated with best available care. Previous studies found that high visceral adipose tissue-to-skeletal muscle ratio is associated with poor outcomes following pancreaticoduodenectomy (PD). The aim of the study is to assess the impact of sarcopenic obesity on occurrence of FTR from major complications in cancer patients undergoing PD...
November 7, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/29106966/single-step-preparation-of-selected-biological-fluids-for-the-high-performance-liquid-chromatographic-analysis-of-fat-soluble-vitamins-and-antioxidants
#18
Giacomo Lazzarino, Salvatore Longo, Angela Maria Amorini, Valentina Di Pietro, Serafina D'Urso, Giuseppe Lazzarino, Antonio Belli, Barbara Tavazzi
Fat-soluble vitamins and antioxidants are of relevance in health and disease. Current methods to extract these compounds from biological fluids mainly need use of multi-steps and multi organic solvents. They are time-consuming and difficult to apply to treat simultaneously large sample number. We here describe a single-step, one solvent extraction of fat-soluble vitamins and antioxidants from biological fluids, and the chromatographic separation of all-trans-retinoic acid, 25-hydroxycholecalciferol, all-trans-retinol, astaxanthin, lutein, zeaxanthin, trans-β-apo-8'-carotenal, γ-tocopherol, β-cryptoxanthin, α-tocopherol, phylloquinone, lycopene, α-carotene, β-carotene and coenzyme Q10...
December 8, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29097063/expression-and-localization-of-polypeptide-n-acetylgalactosaminyltransferase-like-protein-5-in-the-reproductive-organs-and-sperm-of-hu-sheep
#19
Xiaolei Yao, M A Ei-Samahy, Xu Feng, Tinging Zhang, Fengzhe Li, Guomin Zhang, Jing Pang, Haitao Nie, Feng Wang
The polypeptide N-acetylgalactosaminyltransferase-like protein 5 (GALNTL5) is involved in male fertility; however, its involvement in the reproduction and fertility of females remains unclear. Therefore, the present study aimed to examine the presence of GALNTL5 in the reproductive organs of ewes during the estrus period and to investigate its expression in cauda epididymal and ejaculated sperm. Results showed that GALNTL5 mRNA and protein were present in some reproductive organs of ewes during the estrus period...
October 27, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29078039/effectiveness-of-seminal-plasma-in-ivf-treatment-a-systematic-review-and-meta-analysis
#20
Gabriele Saccone, Attilio Di Spiezio Sardo, Andrea Ciardulli, Claudia Caissutti, Marialuigia Spinelli, Daniel Surbek, Michael von Wolff
BACKGROUND: In in vitro fertilization (IVF) techniques only 20 to 25% of the transferred embryos lead to a pregnancy OBJECTIVE: To evaluate the beneficial effects of SP or semen applied at the time of oocyte aspiration or embryo transfer SEARCH STRATEGY: Electronic databases were searched from their inception until August 2017. SELECTION CRITERIA: We included all randomized controlled trials (RCTs) evaluating the effects of SP or semen in IVF treatment. Trials were considered if women were exposed to any kind of SP or semen (either SP/semen injection or sexual intercourse) around the time of oocyte pickup and embryo transfer...
October 27, 2017: BJOG: An International Journal of Obstetrics and Gynaecology
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