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https://www.readbyqxmd.com/read/29036474/casanova-a-multiclass-support-vector-machine-model-for-the-classification-of-human-sperm-motility-patterns
#1
Summer G Goodson, Sarah White, Alicia M Stevans, Sanjana Bhat, Chia-Yu Kao, Scott Jaworski, Tamara R Marlowe, Martin Kohlmeier, Leonard McMillan, Steven H Zeisel, Deborah A O'Brien
The ability to accurately monitor alterations in sperm motility is paramount to understanding multiple genetic and biochemical perturbations impacting normal fertilization. Computer-aided sperm analysis (CASA) of human sperm typically reports motile percentage and kinematic parameters at the population level, and uses kinematic gating methods to identify subpopulations such as progressive or hyperactivated sperm. The goal of this study was to develop an automated method that classifies all patterns of human sperm motility during in vitro capacitation following the removal of seminal plasma...
October 4, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29035835/nerve-growth-factor-beta-purified-from-bull-seminal-plasma-enhances-corpus-luteum-formation-and-conceptus-development-in-bos-taurus-cows
#2
Jamie L Stewart, Vitor R G Mercadante, Nicholas W Dias, Igor F Canisso, Peter Yau, Brian Imai, Fabio S Lima
The objective of the current study was to determine if Nerve Growth Factor-Beta (NGF), purified from bovine seminal plasma, would improve corpus luteum function and enhance conceptus development when administered to cows at artificial insemination. Angus cows (n = 60) were synchronized using a GnRH-prostaglandin and intravaginal progesterone protocol (7-day CO-Synch/CIDR) and randomly allocated to 1 of 2 treatment groups: (1) CONT- 12 mL PBS; or (2) NGF- 296 μg purified NGF in 12 mL PBS administered intramuscularly at insemination (day 0)...
October 7, 2017: Theriogenology
https://www.readbyqxmd.com/read/29029874/sperm-subpopulations-in-ejaculated-sperm-and-spermatozoa-recovered-from-ovine-epididymides-up-to-48h-after-death
#3
Tácia Gomes Bergstein-Galan, Romildo Romualdo Weiss, Luiz Ernandes Kozicki, Sony Dimas Bicudo
The objectives of this study were threefold: to identify subpopulations of sperm based on the kinetics of frozen/thawed sheep epididymal spermatozoa or semen collected with an artificial vagina; to evaluate the effects on sperm subpopulations in the thawed samples of post mortem storage at room temperature and the addition of 20% of seminal plasma to the freezing extender and to correlate the percentage of subpopulations with gestation rate following artificial intrauterine insemination. The categorization of the subpopulations was based on sperm kinetic data from Computer Assisted Sperm Analysis (CASA)...
October 5, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29028777/taboo-in-the-workplace-a-qualitative-study-of-taiwanese-nurses-not-eating-pineapple-in-clinical-practice
#4
Shu-Ling Tsai, Chang-Hsiung Tsai, Yu-Chien Hsu, Mei-Hui Hsieh, Hsia-Tzu Kao, Min-Tao Hsu
There has been an increased emphasis on nurses' mental health and well-being in the workplace. Psychologists have established a correlative link between individual's beliefs on luck and mental health. The pineapple taboo has been observed among Taiwanese hospital nurses as a prevalent superstitious belief for bringing luck or warding off increased clinical workloads, but how and why the ritual persists in the hospital workplace remains unknown. This article aims to explore the latent meaning of observance of the taboo and how it is related to nurses' clinical practice and possibly affects their mental health at work...
November 2017: Holistic Nursing Practice
https://www.readbyqxmd.com/read/29023077/spermatogenic-and-phylo-molecular-characterizations-of-isolated-fasciola-spp-from-cattle-north-west-iran
#5
Soheila Rouhani, Saber Raeghi, Adel Spotin
BACKGROUND AND OBJECTIVE: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. MATERIALS AND METHODS: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/28989661/completing-the-series-of-boron-nucleophilic-cyanoborates-boryl-anions-of-type-nhc-b-cn-2
#6
Richard Böser, Lisa C Haufe, Matthias Freytag, Peter G Jones, Gerald Hörner, René Frank
Since the first seminal report of boron-centred nucleophiles, the area of boryl anions has developed only sporadically and requires further systematisation. The boryl anions of type NHC-B(CN)2(-) (NHC = N-heterocyclic carbene) described herein complete a consistent series with the known anions cAAC-B(CN)2(-) [cAAC = cyclic(alkyl)(amino)carbene] and B(CN)3(2-). A novel approach towards NHC-stabilised cyanoboranes based on alkylthio-cyano exchange at boron is presented, and in contrast to other methods affords the products in better purity and yield...
September 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28988559/ram-semen-deterioration-by-short-term-exposure-to-high-altitude-is-prevented-by-improvement-of-antioxidant-status
#7
E Cofré, O A Peralta, A Raggi, M De Los Reyes, F Sales, A González-Bulnes, V H Parraguez
Ovine reproduction efficiency in herds at high altitude (ha) is lower than that at low altitude (la). In ewes, ha effects are due to hypoxia and oxidative stress. Our aim was to establish the effect of antioxidant vitamin supplementation on semen traits and antioxidant status of rams exposed to short or long time ha. A total of 32 rams native to la (~500 m) were used, 16 were kept at la and the other 16 were brought to ha (~3600 m), where they were placed in the same flock as the ha native rams (n=16). Half of the animals in each group were supplemented daily with vitamins C 600 mg and E 450 IU per os, during the entire experimental period, starting the 4th day after animal's arrival at ha (day 0)...
October 9, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28983945/association-between-promoter-methylation-of-mlh1-and-msh2-and-reactive-oxygen-species-in-oligozoospermic-men-a-pilot-study
#8
S Gunes, A Agarwal, R Henkel, A M Mahmutoglu, R Sharma, S C Esteves, A Aljowair, D Emirzeoglu, A Alkhani, L Pelegrini, A Joumah, E Sabanegh
MLH1 and MSH2 are important genes for DNA mismatch repair and crossing over during meiosis and are implicated in male infertility. Therefore, the methylation patterns of the DNA mismatch repair genes MLH1 and MSH2 in oligozoospermic males were investigated. Ten oligozoospermic patients and 29 normozoospermic donors were analysed. Methylation profiles of the MLH1 and MSH2 promotors were analysed. In addition, sperm motility and seminal reactive oxygen species (ROS) were recorded. Receiver operating characteristic (ROC) analysis was conducted to determine the accuracy of the DNA methylation status of MLH1 and MSH2 to distinguish between oligozoospermic and normozoospermic men...
October 6, 2017: Andrologia
https://www.readbyqxmd.com/read/28981735/pathological-and-oncological-features-of-korean-prostate-cancer-patients-eligible-for-active-surveillance-analysis-from-the-k-cap-registry
#9
Kyo Chul Koo, Kwang Suk Lee, Jae Yong Jeong, In Young Choi, Ji Youl Lee, Jun Hyuk Hong, Choung-Soo Kim, Hyun Moo Lee, Sung Kyu Hong, Seok-Soo Byun, Seung Hwan Lee, Koon Ho Rha, Byung Ha Chung
Background: A web-based multicenter Korean Prostate Cancer Database (K-CaP) was established to provide urologists with information on Korean prostate cancer (PCa) patients treated with radical prostatectomy (RP). We utilized the K-CaP registry to identify pathological features and oncological outcomes of Korean PCa patients eligible for active surveillance (AS). Methods: The K-CaP registry consisted of 6415 patients who underwent RP from May 2001 to April 2013 at five institutions...
October 1, 2017: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28969406/effect-of-superoxide-dismutase-supplementation-on-sperm-dna-fragmentation
#10
Luciano Negri, Renzo Benaglia, Emanuela Monti, Emanuela Morenghi, Alessandro Pizzocaro, Paolo E Levi Setti
BACKGROUND: antioxidants supplementation improves sperm quality, but few trials have analyzed the effects on sperm DNA fragmentation (SDF). This study compares the effectiveness of SOD-based antioxidant supplementation plus hydroxytyrosol and carnosol in reducing SDF with other antioxidants without SOD, hydroxytyrosol, and carnosol. MATERIALS AND METHODS: men with high SDF at baseline were selected in our clinical database. The patients taken into account had a 2-month control...
October 3, 2017: Archivio Italiano di Urologia, Andrologia
https://www.readbyqxmd.com/read/28968457/natural-course-of-the-nodular-bronchiectatic-form-of-mycobacterium-avium-complex-lung-disease-long-term-radiologic-change-without-treatment
#11
Tae Yun Park, Semin Chong, Jae-Woo Jung, In Won Park, Byoung Whui Choi, Changwon Lim, Chang Un Lee, Yang Soo Kim, Hye Won Choi, Jae Chol Choi
BACKGROUND/PURPOSE: Although the incidence of Mycobacterium avium complex (MAC) lung disease is increasing, the long-term natural course of the nodular bronchiectatic form of MAC lung disease is not well described. The objective of our study is to evaluate long-term radiologic changes in untreated MAC lung disease by analyzing serial chest computed tomography (CT) scan findings. METHODS: Of 104 patients with MAC lung disease, we selected 40 untreated nodular bronchiectatic MAC patients who underwent serial chest CTs without treatment for at least four years (mean = 6...
2017: PloS One
https://www.readbyqxmd.com/read/28968199/meta-analysis-of-central-and-peripheral-%C3%AE-aminobutyric-acid-levels-in-patients-with-unipolar-and-bipolar-depression
#12
Bruno Romeo, Walid Choucha, Philippe Fossati, Jean-Yves Rotge
BACKGROUND: Many studies have measured central and peripheral γ-aminobutyric acid (GABA) levels in patients with depression. We performed a meta-analysis to provide an objective overview of GABA changes in those with unipolar or bipolar depression. METHODS: After a systematic database search, original data were extracted with the help of seminal authors to calculate standardized mean differences. We compared GABA levels between patients with current major depressive episodes and controls, between euthymic patients and controls, and in patients before and after treatment...
October 2, 2017: Journal of Psychiatry & Neuroscience: JPN
https://www.readbyqxmd.com/read/28964767/the-angiotensin-converting-enzyme-ace-inhibitor-captopril-disrupts-the-motility-activation-of-sperm-from-the-silkworm-bombyx-mori
#13
Sumiharu Nagaoka, Saori Kawasaki, Hideki Kawasaki, Kaeko Kamei
Angiotensin I-converting enzyme (also known as peptidyl dicarboxypeptidase A, ACE, and EC 3.4.15.1), which is found in a wide range of organisms, cleaves C-terminal dipeptides from relatively short oligopeptides. Mammalian ACE plays an important role in the regulation of blood pressure. However, the precise physiological functions of insect ACE homologs have not been understood. As part of our effort to elucidate new physiological roles of insect ACE, we herein report a soluble ACE protein in male reproductive secretions from the silkmoth, Bombyx mori...
September 28, 2017: Journal of Insect Physiology
https://www.readbyqxmd.com/read/28960100/corrected-cholesterol-a-novel-marker-for-predicting-semen-post-thaw-quality-a-pilot-study
#14
Chey G Dearing, Kevin S Lindsay
Semen cryopreservation is the recommended method for protecting the fertility of men before they undergo iatrogenic treatments. However, post-thaw sperm quality is highly variable between and within individuals. Thus, prediction of post-thaw quality has not been achieved with great certainty. Men (n = 101) attending our andrology clinic each produced a single ejaculate for semen analysis and semen cryopreservation. Post-thaw semen quality was examined for associations with traditional and novel semen and seminal plasma parameters...
September 29, 2017: Human Fertility: Journal of the British Fertility Society
https://www.readbyqxmd.com/read/28957318/use-of-rhodamine-b-to-mark-the-body-and-seminal-fluid-of-male-aedes-aegypti-for-mark-release-recapture-experiments-and-estimating-efficacy-of-sterile-male-releases
#15
Brian J Johnson, Sara N Mitchell, Christopher J Paton, Jessica Stevenson, Kyran M Staunton, Nigel Snoad, Nigel Beebe, Bradley J White, Scott A Ritchie
BACKGROUND: Recent interest in male-based sterile insect technique (SIT) and incompatible insect technique (IIT) to control Aedes aegypti and Aedes albopictus populations has revealed the need for an economical, rapid diagnostic tool for determining dispersion and mating success of sterilized males in the wild. Previous reports from other insects indicated rhodamine B, a thiol-reactive fluorescent dye, administered via sugar-feeding can be used to stain the body tissue and seminal fluid of insects...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28950441/evaluation-of-sperm-dna-quality-in-men-presenting-with-testicular-cancer-and-lymphoma-using-alkaline-and-neutral-comet-assays
#16
K Kumar, S Lewis, S Vinci, A Riera-Escamilla, M-G Fino, L Tamburrino, M Muratori, P Larsen, C Krausz
Despite more cancers in young men over the past two decades, improvements in therapies give a greater chance to live full lives following treatment. Sperm genomic quality is variable following cancer diagnosis, so its assessment is important if sperm cryopreservation is being considered. Here, we evaluated DNA damage using two DNA damage assays: an alkaline and for the first time, a neutral Comet assays in men presenting with testicular cancer (n = 19 for alkaline and 13 for neutral group) and lymphoma (n = 13 for alkaline and 09 for neutral group) compared with fertile donors (n = 20 for alkaline and 14 for neutral group)...
September 26, 2017: Andrology
https://www.readbyqxmd.com/read/28941917/national-trends-in-the-management-of-primary-vesicoureteral-reflux-in-children
#17
Michael Garcia-Roig, Curtis Tavers, Courtney E McCracken, Andrew J Kirsch
PURPOSE: In September 2011 the AAP (American Academy of Pediatrics) released updated guidelines for the evaluation of children 2 to 24 months old with a febrile urinary tract infection. We documented the impact of the guideline on diagnosis and surgical management of vesicoureteral reflux at U.S. children's hospitals. We hypothesized that voiding cystourethrogram studies and the vesicoureteral reflux treatment rate decreased concurrent with the national guideline release. MATERIALS AND METHODS: The Pediatric Health Information System was queried for children (younger than 18 years) with primary vesicoureteral reflux and their antireflux surgical history from January 2004 to June 2015...
September 20, 2017: Journal of Urology
https://www.readbyqxmd.com/read/28940889/effect-of-sexual-steroids-on-boar-kinematic-sperm-subpopulations
#18
E M E Ayala, M A Aragón
Here, we show the effects of sexual steroids, progesterone, testosterone, or estradiol on motility parameters of boar sperm. Sixteen commercial seminal doses, four each of four adult boars, were analyzed using computer assisted sperm analysis (CASA). Mean values of motility parameters were analyzed by bivariate and multivariate statistics. Principal component analysis (PCA), followed by hierarchical clustering, was applied on data of motility parameters, provided automatically as intervals by the CASA system...
September 20, 2017: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/28922899/the-current-status-of-the-distribution-range-of-the-western-pine-beetle-dendroctonus-brevicomis-curculionidae-solytinae-in-northern-mexico
#19
O Valerio-Mendoza, F Armendáriz-Toledano, G Cuéllar-Rodríguez, José F Negrón, G Zúñiga
The distribution range of the western pine beetle Dendroctonus brevicomis LeConte (Coleoptera: Curculionidae) is supported only by scattered records in the northern parts of Mexico, suggesting that its populations may be marginal and rare in this region. In this study, we review the geographical distribution of D. brevicomis in northern Mexico and perform a geometric morphometric analysis of seminal rod shape to evaluate its reliability for identifying this species with respect to other members of the Dendroctonus frontalis Zimmermann (Coleoptera: Curculionidae) complex...
September 1, 2017: Journal of Insect Science
https://www.readbyqxmd.com/read/28917309/toxic-action-of-acmella-oleracea-extract-on-the-male-reproductive-system-of-amblyomma-cajennense-ticks
#20
Luís Adriano Anholeto, Patrícia Rosa de Oliveira, Rodney Alexandre Ferreira Rodrigues, Lais Thiemi Yamane, Karina Neoob de Carvalho Castro, Allan Roberto Fernandes Ferreira, Maria Izabel Camargo-Mathias
The present study evaluated through morphohistological and histochemical techniques the effects of different concentrations of crude ethanolic extract of A. oleracea (EEAO) (Jambu) on the male reproductive system of Amblyomma cajennense sensu stricto (s.s.) ticks. The toxicity of this natural chemical was stablished, signalizing the promising potential of the compound as a strategy to control ectoparasites in the near future. For the experiment, 100 males fed on host rabbits with homogeneous weight (p>0...
September 15, 2017: Veterinary Parasitology
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