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https://www.readbyqxmd.com/read/28448896/differentially-methylated-embryonal-fyn-associated-substrate-efs-gene-as-a-blood-specific-epigenetic-marker-and-its-potential-application-in-forensic-casework
#1
Athina Vidaki, Cecilia Johansson, Federica Giangasparo
DNA methylation patterns have the ability to reveal the activities of genes within a certain tissue at a particular time point. Tissue-specific DNA methylation patterns have been previously investigated for their applicability in the identification of forensically relevant body fluids, however there is still a lack in robust markers. While following a genome-wide scale investigation has a great potential to reveal useful tissue-specific changes, a gene-targeted approach can also lead to significant outcomes, especially in genomic locations not included in the genome-wide experiments...
April 19, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28448268/the-effect-of-pycnogenol-on-wound-healing-in-diabetic-rats
#2
Elif Dogan, Latif Yanmaz, Semin Gedikli, Ugur Ersoz, Zafer Okumus
Pycnogenol (PYC), an extract of pine bark, is known to have photoprotective, antimicrobial, antioxidant, and anti-inflammatory properties. An in vivo study was conducted to evaluate the effects of PYC treatment on wound healing in 48 adult male Sprague-Dawley rats, of which 24 were injected with a single dose of alloxan to induce diabetes. Three (3) excisional skin wounds (1.3 cm x 1.3 cm x 2 mm) were created in each healthy and diabetic animal. One (1) wound in each animal was left untreated, 1 was treated daily with a cleanser (ethacridine lactate) and covered with silver sulfadiazine (SSD), and 1 was treated with PYC powder (30 mg)...
April 2017: Ostomy/wound Management
https://www.readbyqxmd.com/read/28443587/role-of-trace-elements-for-oxidative-status-and-quality-of-human-sperm
#3
Galina Nenkova, Lubomir Petrov, Albena Alexandrova
BACKGROUND: Oxidative stress affects negatively the sperm quality. For maintaining the pro/antioxidative balance, some metal ions (e.g. copper, zinc, iron, selenium), which are co-factors of the antioxidant enzymes, are essential. However, iron and copper could act as prooxidants inducing oxidative damage of spermatozoa. AIMS: This study aims to reveal a possible correlation between the concentrations of some metal ions (iron, copper, zinc, and selenium) in human seminal plasma, oxidative stress, assessed by malondialdehyde (MDA) and total glutathione (tGSH) levels, and semen quality, assessed by the parameters count, motility, and morphology...
April 6, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28434379/hick-s-law-for-choice-reaction-time-a-review
#4
Robert W Proctor, Darryl W Schneider
In 1952, W. E. Hick published an article in the Quarterly Journal of Experimental Psychology, "On the Rate of Gain of Information." It played a seminal role in the cognitive revolution and established one of the few widely acknowledged laws in psychology, relating choice reaction time to the number of stimulus-response alternatives (or amount of uncertainty) in a task. We review the historical context in which Hick conducted his study and describe his experiments and theoretical analyses. We discuss the article's immediate impact on researchers, as well as challenges to and shortcomings of Hick's law and his analysis, including effects of stimulus-response compatibility, practice, very large set sizes, and sequential dependencies...
April 24, 2017: Quarterly Journal of Experimental Psychology: QJEP
https://www.readbyqxmd.com/read/28431025/1h-magnetic-resonance-spectroscopy-of-live-human-sperm
#5
S Reynolds, S J Calvert, M N Paley, A A Pacey
STUDY QUESTION: Can 1H Magnetic Resonance Spectroscopy (MRS) be used to obtain information about the molecules and metabolites in live human spermatozoa? SUMMARY ANSWER: Percoll-based density gradient centrifugation (DGC) followed by a further two washing steps, yielded enough sperm with minimal contamination (<0.01%) from seminal fluid to permit effective MRS which detected significant differences (p < 0.05) in the choline/glycerophosphocholine, lipid and lactate regions of the 1H MRS spectrum between sperm in the pellet and those from the 40/80% interface...
April 18, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28421619/molecular-detection-of-chlamydia-trachomatis-and-semen-quality-of-sexual-partners-of-infertile-women
#6
M López-Hurtado, M Velazco-Fernández, M J E Pedraza-Sánchez, V R Flores-Salazar, R Villagrana Zesati, F M Guerra-Infante
Chlamydia trachomatis is considered as the bacterium that is more sexually transmitted as cause of male urethritis, epididymitis, orchitis and infertility. A total of 116 semen samples of men whose couples are infertile women were analysed. The quality of the semen was measured by standard procedures recommended by WHO while C. trachomatis was detected by the PCR assay. Thirty-seven semen samples were positive for C. trachomatis (31.9%). Regarding semen analysis, no different values were observed between positive and negative samples to C...
April 19, 2017: Andrologia
https://www.readbyqxmd.com/read/28413807/from-brain-to-blood-vessels-and-back-a-noninvasive-optical-imaging-approach
#7
REVIEW
Gabriele Gratton, Antonio M Chiarelli, Monica Fabiani
The seminal work of Grinvald et al. has paved the way for the use of intrinsic optical signals measured with reflection methods for the analysis of brain function. Although this work has focused on the absorption signal associated with deoxygenation, due to its detailed mapping ability and good signal-to-noise ratio, Grinvald's group has also described other intrinsic signals related to increased blood flow, scattering effects directly related to neural activation, and pulsation effects related to arterial function...
July 2017: Neurophotonics
https://www.readbyqxmd.com/read/28413378/is-age-an-independent-factor-for-prostate-cancer-a-paired-analysis
#8
José Arnaldo Shiomi da Cruz, Carlo C Passerotti, Sabrina Thalita Dos Reis, Mary Ellen Salles Guariero, Olimpio Daniel de Campos, Katia Ramos Moreira Leite, Miguel Srougi
INTRODUCTION: Prostate cancer is the most prevalent malignant neoplasia among men worldwide. Several prognostic factors, including Gleason's score, the measurement of serum prostate-specific antigen (PSA) and the evaluation of the percentage of fragments affected by cancer on prostate biopsy, have already been established. Age alone, however, has yet to be studied as a prognostic factor independently from other known factors. The aim of the present study was to compare the characteristics and the evolution of prostate cancer in different age groups using a paired analysis for patients with equivalent known prognostic factors...
January 2017: Current Urology
https://www.readbyqxmd.com/read/28407790/ultrastructure-and-localization-of-neorickettsia-in-adult-digenean-trematodes-provides-novel-insights-into-helminth-endobacteria-interaction
#9
Kerstin Fischer, Vasyl V Tkach, Kurt C Curtis, Peter U Fischer
BACKGROUND: Neorickettsia are a group of intracellular α proteobacteria transmitted by digeneans (Platyhelminthes, Trematoda). These endobacteria can also infect vertebrate hosts of the helminths and cause serious diseases in animals and humans. Neorickettsia have been isolated from infected animals and maintained in cell cultures, and their morphology in mammalian cells has been described. However, limited information is available on the morphology and localization of Neorickettsia in the trematode host...
April 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28394474/proteomic-identification-of-rainbow-trout-blood-plasma-proteins-and-their-relationship-to-seminal-plasma-proteins
#10
Joanna Nynca, G J Arnold, T Fröhlich, A Ciereszko
The characterization of fish blood proteomes is important for comparative studies of seminal and blood proteins as well as for the analysis of fish immune mechanisms and pathways. In this study LC-MS/MS and 2D DIGE were applied to compare rainbow trout seminal (SP) and blood plasma (BP) proteomes. The 54 differentially abundant proteins identified in SP are involved in a variety of signalling pathways, including protein ubiquitination, liver X receptor/retinoid X receptor (LXR/RXR) and farnesoid X receptor (FXR)/RXR activation, cell cycle and acute phase signalling...
April 10, 2017: Proteomics
https://www.readbyqxmd.com/read/28392474/novel-fsh%C3%AE-mutation-in-a-male-patient-with-isolated-fsh-deficiency-and-infertility
#11
Junjie Zheng, Jangfeng Mao, Mingxuan Cui, Zhaoxiang Liu, Xi Wang, Shuyu Xiong, Min Nie, Xueyan Wu
Isolated follicle stimulating hormone (FSH) deficiency due to mutations in FSHβ is an extremely rare autosomal recessive disease that has only been reported in ten patients to date. Symptoms of the disease include amenorrhoea and hypogonadism in women and azoospermia and normal testosterone levels in men. This study describes a Chinese male patient who presented with cryptorchidism and infertility. His serum hormonal profile revealed low FSH, elevated LH and normal testosterone levels. Sequence analysis identified a novel homozygous mutation in the FSHβ gene (c...
April 6, 2017: European Journal of Medical Genetics
https://www.readbyqxmd.com/read/28385362/twenty-years-after-how-aberration-correction-in-the-stem-truly-placed-a-a-synchrotron-in-a-microscope
#12
Quentin M Ramasse
Nearly two decades have passed since the Electron Microscopy and Analysis Group (EMAG) Conference was held in Cambridge in 1997, during which two seminal lectures were delivered that would influence the future of the U.K. electron microscopy community. With "Aberration correction in the STEM", O.L. Krivanek and co-workers ushered in the era of probe-corrected scanning transmission electron microscopy, a powerful technology that L.M. Brown urged the community at large to embrace, arguing that it would be akin to placing "A Synchrotron in a Microscope"...
April 1, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28380686/efficacy-of-treatment-with-pseudoephedrine-in-men-with-retrograde-ejaculation
#13
O Shoshany, N Abhyankar, J Elyaguov, C Niederberger
The use of pseudoephedrine, an alpha agonist, for the treatment of retrograde ejaculation is well-known, however, there is no clear consensus from the literature regarding its efficacy and treatment protocol. We evaluated the efficacy of pseudoephedrine treatment in patients with retrograde ejaculation, utilizing a yet undescribed short-period treatment protocol. Twenty men were medically treated with pseudoephedrine for retrograde ejaculation between January 2010 and May 2016 (12 with complete retrograde ejaculation and 8 with partial retrograde ejaculation)...
April 5, 2017: Andrology
https://www.readbyqxmd.com/read/28379672/does-index-tumor-predominant-location-influence-prognostic-factors-in-radical-prostatectomies
#14
Athanase Billis, Leandro L L Freitas, Larissa B E Costa, Camila M Angelis, Kelson R Carvalho, Luis A Magna, Ubirajara Ferreira
PURPOSE: To find any influence on prognostic factors of index tumor according to predominant location. MATERIALS AND METHODS: Prostate surgical specimens from 499 patients submitted to radical retropubic prostatectomy were step-sectioned. Each transverse section was subdivided into 2 anterolateral and 2 posterolateral quadrants. Tumor extent was evaluated by a semi-quantitative point-count method. The index tumor (dominant nodule) was recorded as the maximal number of positive points of the most extensive tumor area from the quadrants and the predominant location was considered anterior (anterolateral quadrants), posterior (posterolateral quadrants), basal (quadrants in upper half of the prostate), apical (quadrants in lower half of the prostate), left (left quadrants) or right (right quadrants)...
April 6, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28379343/jnk-and-p38-mapk-pathways-link-capacitation-with-apoptosis-and-seminal-plasma-proteins-protect-sperm-by-interfering-with-both-routes
#15
Carolina Luna, Noelia Mendoza, Adriana Casao, Rosaura Pérez-Pé, José A Cebrián-Pérez, Teresa Muiño-Blanco
The Mitogen-activated protein kinase (MAPK) JNK and p38 signaling cascades are involved in triggering apoptosis in somatic cells. Given that spermatozoa are able to undergo apoptosis, we tested the hypothesis that these pathways might be functional in ram spermatozoa as two signal transduction mechanisms that contribute to the modulation of capacitation and apoptosis. Indirect immunofluorescence and western-blot analysis evidenced the presence of JNK and p38 in ram spermatozoa. To verify the involvement of these enzymes in sperm physiology, we determined the effect of specific inhibitors of JNK or p38 on in vitro capacitation induced with either cAMP-elevating agents or Epidermal Growth Factor (EGF)...
March 31, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28366278/loss-of-protein-kinase-2-subunit-alpha-2-ck2%C3%AE-effect-ram-sperm-function-after-freezing-and-thawing-process
#16
Yuxuan He, Hongyan Li, Ke Wang, Yong Zhang, Xingxu Zhao
Protein kinase 2 subunit alpha 2 (CK2α'), a serine/threonine-selective protein kinase, is associated with sperm apoptosis. However, the presence of CK2α' in ram sperm during the freezing-thawing process has not been previously reported. Thus, this study was conducted to determine the effect of cryopreservation on the association between CK2α' and ram sperm function. Sperm variables, including sperm motility, DNA damage and acrosome integrity, were measured in fresh (F), cooled (CO) and freeze-thawed (FT) sperm...
February 16, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28366276/high-dose-rate-brachytherapy-boost-for-prostate-cancer-analysis-of-dose-volume-histogram-parameters-for-predicting-late-rectal-toxicity
#17
Rodolfo Chicas-Sett, Dolores Farga, Maria J Perez-Calatayud, Francisco Celada, Susana Roldan, Victoria Fornes-Ferrer, Blanca Ibanez-Rosello, Alejandro Tormo, Jose M Benlloch, Jose Perez-Calatayud
PURPOSE: To determine the significance of dose-volume histogram parameters for predicting late rectal toxicity (LRT) after single-fraction high-dose-rate brachytherapy (HDRBT) boost and external beam radiotherapy (EBRT) in prostate cancer. MATERIALS AND METHODS: Three hundred patients with intermediate- or high-risk prostate cancer were included between August 2010 and March 2015. Treatment comprised a single-fraction HDRBT boost of 15.0 Gy plus EBRT (46.0 Gy delivered in 23 fractions) or an HDRBT boost of 9...
March 30, 2017: Brachytherapy
https://www.readbyqxmd.com/read/28360803/exposure-of-students-to-emotional-and-physical-violence-in-the-school-environment
#18
Şahbal Aras, Sema Özan, Sevgi Timbil, Semih Şemin, Oya Kasapçi
INTRODUCTION: While peer abuse or physical violence in school is emphasized more, the physical and emotional violence caused by school staff has been emphasized less. The purpose of this study was to investigate the variables related to emotional and physical violence that students are exposed to in the school environment. METHODS: This cross-sectional and descriptive study was conducted by applying a questionnaire to 434 fifth-grade students receiving education in the primary schools in Konak district of Izmir province...
December 2016: Noro Psikiyatri Arsivi
https://www.readbyqxmd.com/read/28356499/relationship-of-dna-integrity-to-hrg-c633t-snp-and-art-outcome-in-infertile-couples
#19
Arumugam Kumaresan, Anders Johannisson, Sarah Nordqvist, Karin Kårehed, Helena Åkerud, Karin Lindgren, Jane Morrell
The status of sperm DNA fragmentation, protamine deficiency, free thiols and disulphide bonds in colloid-selected samples and its relationship to ART outcome or HRG C633T SNP is not known. The objective of this study was to determine these relationships in spermatozoa from men with male factor or unknown factor infertility (n=118) undergoing IVF or ICSI. Sperm DNA integrity was analyzed by flow cytometry using three fluorescent probes (Acridine Orange, Monobromobimane and Chromomycin A3). Principal component analysis was used to identify the parameters that most influenced fertility...
March 29, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28355113/moderate-hypofractionation-in-high-risk-organ-confined-prostate-cancer-final-results-of-a-phase-iii-randomized-trial
#20
Giorgio Arcangeli, Biancamaria Saracino, Stefano Arcangeli, Sara Gomellini, Maria Grazia Petrongari, Giuseppe Sanguineti, Lidia Strigari
Purpose To report the final results on treatment outcomes of a randomized trial comparing conventional and hypofractionated radiotherapy in high-risk, organ-confined prostate cancer (PCa). Patients and Methods This single-institution, randomized clinical trial, conducted from January 2003 to December 2007, enrolled 168 patients with high-risk PCa who were randomly assigned in a 1:1 ratio to conventional (80 Gy in 40 fractions in 8 weeks) or hypofractionated radiotherapy (62 Gy in 20 fractions in 5 weeks) to prostate and seminal vesicles...
March 29, 2017: Journal of Clinical Oncology: Official Journal of the American Society of Clinical Oncology
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