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seminal analysis

Francesco Porpiglia, Matteo Manfredi, Fabrizio Mele, Riccardo Bertolo, Enrico Bollito, Dario Gned, Agostino De Pascale, Filippo Russo, Roberto Passera, Cristian Fiori, Stefano De Luca
BACKGROUND: The Briganti updated nomogram (BN) is the most popular predictive model aiming to predict the presence of lymph node invasion (LNI) in patients with prostate cancer (PCa), but it lacks information obtained by preoperative imaging. The primary aim of the study was to evaluate the role of multiparametric prostate magnetic resonance imaging (mp-MRI) in the indication to perform pelvic lymph nodes dissection (PLND) or not in patients with risk of LNI according to BN below 5%. METHODS: Since March 2012 and September 2016, 310 patients who underwent a preoperative mp-MRI for staging purpose and subsequent robot-assisted extended PLND (RAEPLND) were retrospectively evaluated...
February 22, 2018: Prostate Cancer and Prostatic Diseases
M-H Zhang, L-P Zhai, Z-Y Fang, A-N Li, Y Qiu, Y-X Liu
The aim of this study was to observe sperm aneuploidy, DNA integrity, seminal alpha-glucosidase (NAG) and acrosin activity (AA) under testicular heat stress (SH). Spermatozoa were obtained from 30 healthy adult volunteers subjected to scrotal warming at 43°C for 30-40 min on two successive days per week for 3 months between February 2012 and September 2016. Aniline blue (AB), acridine orange (AO) staining, TUNEL assay and FISH analysis to evaluate sperm function, sperm DNA integrity and chromosomal abnormalities were carried on before, during and after SH...
February 22, 2018: Andrologia
Debashis Sahoo, Wei Wei, Heidi Auman, Antonio Hurtado-Coll, Peter R Carroll, Ladan Fazli, Martin E Gleave, Daniel W Lin, Peter S Nelson, Jeff Simko, Ian M Thompson, Robin J Leach, Dean A Troyer, Lawrence D True, Jesse K McKenney, Ziding Feng, James D Brooks
The introduction of serum Prostate Specific Antigen (PSA) testing nearly 30 years ago has been associated with a significant shift towards localized disease and decreased deaths due to prostate cancer. Recognition that PSA testing has caused over diagnosis and over treatment of prostate cancer has generated considerable controversy over its value, and has spurred efforts to identify prognostic biomarkers to distinguish patients who need treatment from those that can be observed. Recent studies show that cancer is heterogeneous and forms a hierarchy of tumor cell populations...
January 19, 2018: Oncotarget
B F Zanetti, D P A F Braga, R R Provenza, R C S Figueira, A Iaconelli, E Borges
Human sperm morphology has been described as an essential parameter for the diagnosis of male infertility and a prognostic indicator of natural or assisted pregnancies. Nevertheless, standard morphological assessment remains a subjective analysis and its impact on intracytoplasmic sperm injection (ICSI) is also of limited value. The objective of this prospective cohort study was to investigate whether motile sperm organelle morphology examination (MSOME) can improve semen analysis by better defining male infertility and providing a better prognosis for ICSI up to a year later...
February 18, 2018: Andrology
Houyang Chen, Tao Luo, Tingtao Chen, Gongxian Wang
A number of culture-dependent and -independent studies have reported that the number and significance of bacterial species in semen may have been underestimated. The aim of the present study was to profile the seminal microbiome in patients with obstructive or non-obstructive azoospermia. A high-throughput sequencing method was adopted to sequence genomic DNA extracted from the semen of healthy people (C group), patients with obstructive azoospermia (OA group) and patients with non-obstructive azoospermia (NOA group)...
March 2018: Experimental and Therapeutic Medicine
Banafshe Hosseini, Mahdieh Nourmohamadi, Shima Hajipour, Mohsen Taghizadeh, Zatollah Asemi, Seyed Ali Keshavarz, Sadegh Jafarnejad
The objective was to evaluate the effect of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) on sperm parameters including total sperm concentration, sperm motility, sperm DHA, and seminal plasma DHA concentration in infertile men. The literature search was conducted in PubMed, Google Scholar, and Scopus from January 1, 1990 to December 20, 2017. The systematic review and meta-analysis were based on randomized controlled trials in infertile men with DHA or EPA treatments, either alone or in combination with other micronutrients...
February 16, 2018: Journal of Dietary Supplements
Michelle L McDonald, Lauren E Howard, William J Aronson, Martha K Terris, Matthew R Cooperberg, Christopher L Amling, Stephen J Freedland, Christopher J Kane
OBJECTIVE: To analyze factors associated with metastases, prostate cancer-specific mortality, and all-cause mortality in pN1 patients. MATERIALS AND METHODS: We analyzed 3,642 radical prostatectomy patients within the Shared Equal Access Regional Cancer Hospital (SEARCH) database. Pathologic Gleason grade, number of lymph nodes (LN) removed, and first postoperative prostate-specific antigen (PSA) (<0.2 ng/ml or ≥0.2 ng/ml) were among covariates assessed. Cox regression was used to analyze the association between characteristics and survival outcomes...
February 8, 2018: Urologic Oncology
Herlon Victor Rodrigues Silva, Paula Rodriguez-Villamil, Francisco Felipe de Magalhães, Thalles Gothardo Pereira Nunes, Luana Azevedo de Freitas, Leandro Rodrigues Ribeiro, Alexandre Rodrigues Silva, Arlindo A Moura, Lúcia Daniel Machado da Silva
Ring-tailed coati is listed as a species of least concern in the International Union for Conservation of Nature (IUCN) Red List, however, there has been a sharp decline in their population. The present study was conducted to evaluate the major proteins of both seminal plasma and sperm in ring-tailed coatis. Semen sample was collected from three adult coatis and evaluated for their morphological characteristics. Further, the sample was centrifuged to separate spermatozoa from seminal plasma, and then stored in liquid nitrogen...
January 31, 2018: Theriogenology
Leonardo Lancia, Benjamin Rosenbaum
Since the seminal works of Bernstein (The coordination and regulation of movements. Pergamon Press, Oxford, 1967) several authors have supported the idea that, to produce a goal-oriented movement in general, and a movement of the organs responsible for the production of speech sounds in particular, individuals activate a set of coupling relations that coordinate the behavior of the elements of the motor system involved in the production of the target movement or sound. In order to characterize the configurations of the coupling relations underlying speech production articulator movements, we introduce an original method based on recurrence analysis...
February 9, 2018: Biological Cybernetics
Cristina Nerin, Elena Canellas, Paula Vera, Estefanía Garcia-Calvo, José Luis Luque-Garcia, Carmen Cámara, Raquel Ausejo, Joaquín Miguel, Noelia Mendoza
Migration from a multilayer plastic material intended for food contact showed that 2,4,7,9-tetramethyl-5-decyne-4,7-diol mixture (surfynol), used as a surfactant in the adhesive employed to build the multilayer, was transferred to water and other food simulants in contact with the plastic. When these multilayer plastics were used for containing seminal doses for artificial insemination, it was found that fertility was seriously damaged in terms of motility, acrosome integrity, mitochondrial activity and penetration capacity in the cells, thus affecting male fertility...
January 30, 2018: Food and Chemical Toxicology
Cristina Pérez-Patiño, Inmaculada Parrilla, Isabel Barranco, María Vergara-Barberán, Ernesto F Simó-Alfonso, José M Herrero-Martínez, Heriberto Rodriguez-Martínez, Emilio A Martínez, Jordi Roca
A complete characterization of the proteome of seminal plasma (SP) is an essential step to understand how SP influences sperm function and fertility after artificial insemination (AI). The purpose of this study was to identify which among characterized proteins in boar SP were differently expressed among AI boars with significantly different fertility outcomes. A total of 872 SP proteins, 390 of them belonging specifically to Sus Scrofa taxonomy, were identified (Experiment 1) by using a novel proteomic approach that combined size exclusion chromatography and solid-phase extraction as prefractionation steps prior to Nano LC-ESI-MS/MS analysis...
February 13, 2018: Journal of Proteome Research
John E Schiel, Abby Turner, Trina Mouchahoir, Katharina Yandrofski, Srivalli Telikepalli, Jason King, Paul DeRose, Dean Ripple, Karen Phinney
The NISTmAb Reference Material (RM) 8671 is intended to be an industry standard monoclonal antibody for pre-competitive harmonization of best practices and designing next generation characterization technologies for identity, quality, and stability testing. It must therefore embody the quality and characteristics of a typical biopharmaceutical product and be available long-term in a stable format with consistent product quality attributes. A stratified sampling and analysis plan using a series of qualified analytical and biophysical methods is described that assures RM 8671 meets these criteria...
February 7, 2018: Analytical and Bioanalytical Chemistry
L K Hatamoto-Zervoudakis, M F Duarte Júnior, J T Zervoudakis, T F Motheo, R P Silva-Marques, P P Tsuneda, M Nichi, B S E Santo, R D Almeida
The aim of the present study was to analyze seminal quality of young bulls subjected to different frequencies of gossypol supplementation. Forty-eight Nellore bulls, with 19 months of age and weighing 357.8 ± 7.2 kg, were used in this study. Animals were fed with 10.5 kg of standard supplement containing free-gossypol from whole cottonseed (WCS) at the following frequency: 3x/week (G3x), 5x/week (G5x) or 7x/week (G7x - Control). Additionally, a negative control was provided, and the treated animals received only mineral supplement (MM) ad libtum...
January 9, 2018: Theriogenology
V Tufarelli, A Rizzo, G M Lacalandra, A C Guaricci, V Laudadio, L Valentini
The aim of this work was to evaluate the effects of the supplementation of two extra-virgin olive oils (EVOO) having different polyphenols content, on canine spermatozoa kinetic parameters and seminal plasma oxidative status. The study was conducted on 12 clinically healthy dogs of different breeds (2-7 years, 5-48 kg of body weight) divided into two groups: an experimental group supplemented with EVOO (Coratina cultivar) high in polyphenols (H-P) and a control group fed EVOO (Cima di Bitonto cultivar) low in polyphenols (L-P)...
January 30, 2018: Reproduction in Domestic Animals, Zuchthygiene
Robert Kuo-Kuang Lee, Huan-Chin Tseng, Yuh-Ming Hwu, Chi-Chen Fan, Ming-Huei Lin, Jhih-Jie Yu, Ling-Yu Yeh, Sheng-Hsiang Li
BACKGROUND: Cystatin C (CST3), a cysteine protease inhibitor in seminal plasma, is expressed in animal uteri. However, its expression in the human female reproductive tract and its effect on human sperm capacitation are unclear. METHODS: The cellular localization of CST3 was observed using immunohistochemistry. The binding of CST3 to sperm was examined using immunocytochemistry. Sperm motility parameters were analyzed using computer-assisted sperm analysis. Sperm capacitation was evaluated by analyzing cholesterol content, protein tyrosine phosphorylation levels, and the acrosome reaction...
January 30, 2018: Reproductive Biology and Endocrinology: RB&E
E M Kratz, A Piwowar
Currently, in highly developed, industrialized countries male factors are identified as the primary cause of infertility in about 60% of childless couples. Standard semen analysis parameters, such as sperm morphology, number and motility, are important in predicting the fertility of large populations, but they are not sufficient to fully specify a particular donor sperm's ability to fertilize the egg. The semen also comprises components, which may also affect sperm fertilizing ability and which have thus far remained little explored: the biochemical parameters of the seminal plasma secreted by the testis, the seminiferous tubules and the prostate gland, such as: matrix metalloproteinases (MMP-2 and MMP-9) and their specific tissue inhibitors (TIMP-1 and TIMP-2)...
October 2017: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
Raquel Xavier, Ricardo Severino, Marcos Pérez-Losada, Camino Gestal, Rita Freitas, D James Harris, Ana Veríssimo, Daniela Rosado, Joanne Cable
BACKGROUND: The Apicomplexa from aquatic environments are understudied relative to their terrestrial counterparts, and the seminal work assessing the phylogenetic relations of fish-infecting lineages is mostly based on freshwater hosts. The taxonomic uncertainty of some apicomplexan groups, such as the coccidia, is high and many genera were recently shown to be paraphyletic, questioning the value of strict morphological and ecological traits for parasite classification. Here, we surveyed the genetic diversity of the Apicomplexa in several commercially valuable vertebrates from the North-East Atlantic, including farmed fish...
January 25, 2018: Parasites & Vectors
T Mostafa, N Nabil, L Rashed, K Makeen, M A El-Kasas, H A Mohamaed
In a case-controlled study, we assessed the expressed seminal NAD-dependent protein deacetylase (SIRT1) expression in infertile oligoasthenoteratozoospermic (OAT) men associated with varicocoele. Our study involved 81 men, recruited from the University hospitals, after ethical approval and informed consent. They were allocated into fertile normozoospermic men (n = 23), infertile OAT men without varicocoele (n = 23) and infertile OAT men with varicocoele (n = 35). Inclusion criteria consisted of confirmation of abnormal semen parameters and normal female partners whereas exclusion criteria were leukocytospermia, tobacco smoking, hormonal therapy, immunological disorders, dyslipidemia, hypogonadism, cardiovascular disorders, morbid obesity, and hepatic or renal failures...
January 22, 2018: Andrology
Jackson R Roberts, Cova R Arias, Kenneth M Halanych, Binh T Dang, Stephen A Bullard
Platt sinuosus Roberts & Bullard n. g., n. sp. (type-species) infects the kidney and mesenteric blood vessels of Mekong snail-eating turtles, Malayemys subtrijuga (Schlegel & Müller), in the Mekong River Basin. Species of Platt Roberts & Bullard n. g. are unique by the combination of having a papillate ventral sucker, vasa efferentia that are dorsal to the gonads, a massive cirrus-sac that is directed anteriad or laterad, and a vitellarium that surrounds the intestinal caeca. The new species resembles Platt ocadiae (Takeuti, 1942) Roberts & Bullard n...
January 22, 2018: Systematic Parasitology
Zimbul Albo, Johannes Gräff
Long-lasting memories form the basis of our identity as individuals and lie central in shaping future behaviours that guide survival. Surprisingly, however, our current knowledge of how such memories are stored in the brain and retrieved, as well as the dynamics of the circuits involved, remains scarce despite seminal technical and experimental breakthroughs in recent years. Traditionally, it has been proposed that, over time, information initially learnt in the hippocampus is stored in distributed cortical networks...
March 19, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
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