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seminal analysis

Giuseppe Grande, Federica Vincenzoni, Francesca Mancini, Silvia Baroni, Giovanni Luca, Riccardo Calafiore, Riccardo Marana, Massimo Castagnola, Alfredo Pontecorvi, Domenico Milardi
Infectious etiologies contribute to 15% of male factor infertility. Enterococcus faecalis is commonly identified in semen colture of infertile men and it is associated with significantly poorer semen quality. Aim of this study was to identify new seminal biomarkers for the male tract infection by E. faecalis, using proteomic profiling, in order to understand the effect of E. faecalis on the physiopathology of male reproduction. We included in the study ten patients seeking medical care for primary infertility with prostate-vescicular-epidydimitis and with microbiological analysis on semen and/or prostatic secretions positive for Enterococcus faecalis...
April 12, 2018: Protein and Peptide Letters
Ada Soler-Ventura, Judit Castillo, Alberto de la Iglesia, Meritxell Jodar, Ferran Barrachina, Josep Lluis Ballesca, Rafael Oliva
Protamines are the most abundant sperm nuclear proteins and pack approximately the 92-98% of the mammalian sperm DNA. In mammals, two types of protamines have been described, the protamine 1 (P1) and the protamine 2 (P2) family, whose relative ratio has been related to the proper sperm chromatin maturity and the normality/abnormality of the male gamete. Specifically, the deregulation of the P1/P2 ratio has been correlated to DNA damage, alterations in seminal parameters, and low success rate of assisted reproduction techniques...
April 12, 2018: Protein and Peptide Letters
Ji Hoon Phi, Ae Kyung Park, Semin Lee, Seung Ah Choi, In-Pyo Baek, Pora Kim, Eun-Hye Kim, Hee Chul Park, Byung Chul Kim, Jong Bhak, Sung-Hye Park, Ji Yeoun Lee, Kyu-Chang Wang, Dong-Seok Kim, Kyu Won Shim, Se Hoon Kim, Chae-Yong Kim, Seung-Ki Kim
Despite great advances in understanding of molecular pathogenesis and achievement of a high cure rate in medulloblastoma, recurrent medulloblastomas are still dismal. Additionally, misidentification of secondary malignancies due to histological ambiguity leads to misdiagnosis and eventually to inappropriate treatment. Nevertheless, the genomic characteristics of recurrent medulloblastomas are poorly understood, largely due to a lack of matched primary and recurrent tumor tissues. We performed a genomic analysis of recurrent tumors from 17 pediatric medulloblastoma patients...
April 11, 2018: Acta Neuropathologica
Ketan K Badani, Balaji N Reddy, Eric J Moskowitz, David J Paulucci, Alp Tuna Beksac, Alberto Martini, Michael J Whalen, Douglas W Skarecky, Linda My Huynh, Thomas E Ahlering
OBJECTIVES: Seminal vesicle invasion (SVI) is a risk factor for poor oncologic outcome in patients with prostate cancer. Modifications to the pelvic lymph node dissection (PLND) during radical prostatectomy (RP) have been reported to have a therapeutic benefit. The present study is the first to determine if lymph node yield (LNY) is associated with a lower risk of biochemical recurrence (BCR) for men with SVI. METHODS: A total of 220 patients from 2 high-volume institutions who underwent RP without adjuvant treatment between 1990 and 2015 and had prostate cancer with SVI (i...
April 3, 2018: Urologic Oncology
Tamar Schlick
The structure of RNA has been a natural subject for mathematical modeling, inviting many innovative computational frameworks. This single-stranded polynucleotide chain can fold upon itself in numerous ways to form hydrogen-bonded segments, imperfect with single-stranded loops. Illustrating these paired and non-paired interaction networks, known as RNA's secondary (2D) structure, using mathematical graph objects has been illuminating for RNA structure analysis. Building upon such seminal work from the 1970s and 1980s, graph models are now used to study not only RNA structure but also describe RNA's recurring modular units, sample the conformational space accessible to RNAs, predict RNA's three-dimensional folds, and apply the combined aspects to novel RNA design...
April 2, 2018: Methods: a Companion to Methods in Enzymology
Thanapol Nongbua, Essraa M Al-Essawe, Anders Edman, Anders Johannisson, Jane M Morrell
Variations in fertility between bulls with comparable sperm quality could be due to differences in their seminal plasma (SP). The aim of this study was to investigate the effect of adding bovine SP from bulls of known fertility to SP-free sperm samples. After removal of SP by Single Layer Centrifugation, resuspended sperm pellets were treated with SP from high or low fertility bulls at 0% (control), 1%, or 5% before freezing. Sperm quality was evaluated after thawing. Data were analyzed using Proc MIXED, SAS®...
April 5, 2018: Systems Biology in Reproductive Medicine
Jungeun Kim, Jessica A Weber, Sungwoong Jho, Jinho Jang, JeHoon Jun, Yun Sung Cho, Hak-Min Kim, Hyunho Kim, Yumi Kim, OkSung Chung, Chang Geun Kim, HyeJin Lee, Byung Chul Kim, Kyudong Han, InSong Koh, Kyun Shik Chae, Semin Lee, Jeremy S Edwards, Jong Bhak
High-coverage whole-genome sequencing data of a single ethnicity can provide a useful catalogue of population-specific genetic variations, and provides a critical resource that can be used to more accurately identify pathogenic genetic variants. We report a comprehensive analysis of the Korean population, and present the Korean National Standard Reference Variome (KoVariome). As a part of the Korean Personal Genome Project (KPGP), we constructed the KoVariome database using 5.5 terabases of whole genome sequence data from 50 healthy Korean individuals in order to characterize the benign ethnicity-relevant genetic variation present in the Korean population...
April 4, 2018: Scientific Reports
Etienne Lavallée, Michelle Bergeron, François-Alexandre Buteau, Annie-Claude Blouin, Nicolas Duchesnay, Thierry Dujardin, Rabi Tiguert, Louis Lacombe, Vincent Fradet, Molière Makao-Nguile, Yves Fradet, Jean-Mathieu Beauregard, Frédéric Pouliot
BACKGROUND: The accuracy of18 F-fluorodeoxyglucose positron emission tomography/computed tomography (FDG-PET/CT) to stage prostate cancer (PCa) is limited. However, Gleason 8-10 PCa and more aggressive metastatic PCa have been shown to exhibit a higher glycolytic activity. OBJECTIVE: To evaluate the potential of intraprostatic FDG uptake to prognose Gleason 8-10 PCa patients prior to prostatectomy, based on tumour intrinsic biology. DESIGN, SETTING, AND PARTICIPANTS: FDG-PET/CT and a bone scan were performed as a staging procedure prior to prostatectomy in 148 consecutive patients diagnosed with PCa with a Gleason sum of ≥8 at biopsy...
March 30, 2018: European Urology Focus
N Sarova, S Ahlawat, A Grewal, R Sharma, R Arora
In India, cross-breeding of indigenous cattle with exotic cattle such as Holstein Friesian and Jersey has been going on since last four decades to improve milk production. Although it has led to increased milk yield, the subfertility in male cross-bred progeny has remained a significant problem. Epigenetic modifications (DNA methylation, histone modifications and chromatin remodelling) are regarded as key players influencing gene expression. DAZL gene plays an important role in germline development and gametogenesis...
March 31, 2018: Reproduction in Domestic Animals, Zuchthygiene
C E Fedorka, K E Scoggin, Y L Boakari, N E Hoppe, E L Squires, B A Ball, M H T Troedsson
The deposition of semen into the uterus of the horse induces a transient innate immune response that lasts 24-36 h in the normal mare. There exists a subset of mares that are unable to resolve this inflammation in a timely manner, and are classified as susceptible to the disease of persistent breeding-induced endometritis (PBIE). Lactoferrin is a protein of interest as a potential therapeutic for this persistent inflammation due to its anti-inflammatory and bactericidal properties. The addition of human recombinant lactoferrin (hrLF) to the insemination dose was previously shown to suppress mRNA expression of the pro-inflammatory cytokine tumor necrosis factor (TNF)-α at 6 h after insemination, but no studies have shown the effect of lactoferrin when infused post-breeding...
March 22, 2018: Theriogenology
Bernadett Palyi, Nora Magyar, Judit Henczko, Balint Szalai, Agnes Farkas, Thomas Strecker, Maria Takacs, Zoltan Kis
In 2013-2016, West Africa experienced the largest and longest Ebola virus disease outbreak ever documented. The wide geographic spread and magnitude of the outbreak often limited the timely and rapid testing of diagnostic samples from patients with suspected Ebola virus disease, raising questions regarding the optimal storage and shipping conditions of clinically relevant specimens, including EDTA-whole blood, plasma, capillary blood, urine and seminal fluid (associated with sexual transmission of the Ebola virus after recovery from the disease)...
March 29, 2018: Emerging Microbes & Infections
Qing-Shui Zheng, Shao-Hao Chen, Yu-Peng Wu, Hui-Jun Chen, Hong Chen, Yong Wei, Xiao-Dong Li, Jin-Bei Huang, Xue-Yi Xue, Ning Xu
Purpose Paxillin regulates cell-cell adhesion, and altered Paxillin expression has been associated with human carcinogenesis. This study analyzed the association between Paxillin expression in prostate cancer (PCa) tissues with clinicopathological features, lymph node metastasis and biochemical PCa recurrence. Methods A total of 386 tissue specimens from PCa patients who received radical prostatectomy and 60 tissue specimens from benign prostatic hyperplasia (BPH) cases were collected to construct tissue microarrays, which were subsequently immunostained for Paxillin expression...
2018: Journal of Cancer
Yoshikazu Maeda, Yoshitaka Sato, Satoshi Shibata, Sayuri Bou, Kazutaka Yamamoto, Hiroyasu Tamamura, Nobukazu Fuwa, Shigeyuki Takamatsu, Makoto Sasaki, Yuji Tameshige, Kyo Kume, Hiroki Minami, Yusuke Saga, Makoto Saito
PURPOSE: We quantified interfractional movements of the prostate, seminal vesicles (SVs) and rectum during computed tomography (CT) image-guided proton therapy for prostate cancer and studied the range variation of opposed lateral proton beams. MATERIALS/METHODS: We analyzed 375 sets of daily CT images acquired throughout the proton therapy treatment of 10 patients. We analyzed daily movements of the prostate, SVs and rectum by simulating three image-matching strategies: bone matching, prostate center (PC) matching, and prostate-rectum boundary (PRB) matching...
March 24, 2018: Medical Physics
Tairo Kashihara, Satoshi Nakamura, Akihisa Wakita, Hiroyuki Okamoto, Koji Inaba, Rei Umezawa, Satoshi Shima, Keisuke Tsuchida, Kazuma Kobayashi, Kana Takahashi, Naoya Murakami, Yoshinori Ito, Hiroshi Igaki, Hiroyuki Fujimoto, Takashi Uno, Jun Itami
The aim of this study was to examine long-term outcomes in patients who received salvage radiotherapy (SRT) for biochemical recurrence (BRec) of prostate cancer after radical prostatectomy (RP). One hundred and twenty patients with prostate cancer who underwent SRT for BRec after RP without evidence of clinical disease were identified in our institution from 2002 to 2014. Prescription doses to prostate beds were 64.8 Gy with a fractional dose of 1.8 Gy in 96.7% of the patients. In three-dimensional conformal radiation therapy (3D-CRT), the seminal vesicle bed (SVB) was not included in the radiation fields...
March 23, 2018: Cancer Medicine
J P Barbas, T Leahy, A E Horta, M García-Herreros
Sperm cryopreservation in goats has been a challenge for many years due to the detrimental effects of seminal plasma enzymes produced by the bulbo-urethral glands which catalyse the hydrolysis of lecithins in egg yolk to fatty acids and lysolecithins which are deleterious to spermatozoa. This fact implies to carry out additional processing steps during sperm cryopreservation for seminal plasma removal triggering different sperm responses which may affect sperm functionality. The objective of the present study was to determine specific sperm subpopulation responses in different handling steps during the cryopreservation process by using functional sperm kinematic descriptors in caprine ejaculates...
March 20, 2018: Cryobiology
Olga Serra, Raffaele Frazzi, Alessio Perotti, Lorenzo Barusi, Annamaria Buschini
FTA® technologies provide the most reliable method for DNA extraction. Although FTA technologies have been widely used for genetic analysis, there is no literature on their use for epigenetic analysis yet. We present for the first time, a simple method for quantitative methylation assessment based on sperm cells stored on Whatman FTA classic cards. Specifically, elution of seminal DNA from FTA classic cards was successfully tested with an elution buffer and an incubation step in a thermocycler. The eluted DNA was bisulfite converted, amplified by PCR, and a region of interest was pyrosequenced...
February 1, 2018: BioTechniques
Petra Schwille
What is life? There is hardly a more fundamental question raised by aspiring researchers, and one less prone to ever be answered in a scientifically satisfying way. In the long, productive and highly influential period of research following his Nobel-recognised work on relaxation kinetics, Manfred Eigen made seminal contributions towards a quantifiable definition of life, with a strong focus on its evolutionary character. In the last years of his time as an active researcher, however, he devoted himself to another, purely experimental topic: the detection and analysis of single biomolecules in aqueous solution...
March 22, 2018: European Biophysics Journal: EBJ
Renato Zampini, Luciana M Sari, Martin E Argañaraz, Fernanda G Fumuso, Daniela E Barraza, María I Carretero, Silvana A Apichela
Ovulation of South American Camelids is induced by mating. After copulation, sperm are stored into the oviduct to be released near ovulation time. To study whether copulation induces matrix metalloproteinase-2 (MMP2) secretion in the oviduct, the occurrence of MMP2 in oviductal tissue and oviductal fluid (OF) from 24 h post-mated was compared with non-mated llama females. There was an incremental increase of MMP2 in the oviductal epithelial cells, and MMP2 activity in OF after copulation. Additionally, MMP2 activator (MMP14), inducer (EMMPRIN) and inhibitor (TIMP2) were present in the oviductal epithelial cells of both non-mated and post-mated females...
March 15, 2018: Animal Reproduction Science
Mathew J Tomlinson, Asad Naeem
CASA has been used in reproductive medicine and pathology laboratories for over 25 years, yet the 'fertility industry' generally remains sceptical and has avoided automation, despite clear weaknesses in manual semen analysis. Early implementers had difficulty in validating CASA-Mot instruments against recommended manual methods (haemocytometer) due to the interference of seminal debris and non-sperm cells, which also affects the accuracy of grading motility. Both the inability to provide accurate sperm counts and a lack of consensus as to the value of sperm kinematic parameters appear to have continued to have a negative effect on CASA-Mot's reputation...
March 21, 2018: Reproduction, Fertility, and Development
Chau Le Bao Ho, Monique Breslin, Jenny Doust, Christopher M Reid, Mark R Nelson
OBJECTIVES: In many current guidelines, blood pressure (BP)-lowering drug treatment for primary prevention of cardiovascular disease (CVD) is based on absolute risk. However, in clinical practice, therapeutic decisions are often based on BP levels alone. We sought to investigate which approach was superior by conducting a post hoc analysis of the Australian National Blood Pressure (ANBP) cohort, a seminal study establishing the efficacy of BP lowering in 'mild hypertensive' persons. DESIGN: A post hoc subgroup analysis of the ANBP trial results by baseline absolute risk tertile...
March 19, 2018: BMJ Open
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