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Akram Vatannejad, Heidar Tavilani, Mohammad Reza Sadeghi, Saeid Amanpour, Somyeh Shapourizadeh, Mahmood Doosti
PURPOSE: The aim of study was to evaluate reactive oxygen species (ROS), total antioxidant capacity (TAC) andROS-TAC score as indicator for oxidative stress status as well as 8-hydrodeoxyguanosine (8-OHdG) levels as amarker for DNA damage in the seminal plasma of asthenozoospermia patients compared to normozoospermiasamples. MATERIALS AND METHODS: The semen samples of 28 fertile normozoospermic donors and 25 infertile men withasthenozoospermia were analyzed according to World Health Organization (WHO) criteria...
January 18, 2017: Urology Journal
C D Anfuso, M Olivieri, S Bellanca, M Salmeri, C Motta, M Scalia, C Satriano, S La Vignera, N Burrello, N Caporarello, G Lupo, A E Calogero
Phosholipase A2 (PLA2 ) activity in the seminal plasma and in sperm heads is closely related to sperm motility and male fertility. Therefore, the purpose of this study was to investigate the possible involvement of different isoforms of phospholipase in asthenozoospermia. To accomplish this, cPLA2 , phospho-cPLA2 , iPLA2 , and sPLA2 were evaluated by immunofluorescence and immunoblot analyses in spermatozoa obtained from 22 normozoospermic men and 28 asthenozoospermic patients. We found significant differences in cPLA2 and its phosphorylated/activated form, iPLA2 , and sPLA2 content and distribution in normal and asthenozoospermic patients...
November 2015: Andrology
M R R Zainul, F B Ong, M H Omar, S P Ng, A Nurshaireen, M D Rahimah, S Mariam, M Zolaidah, N S M N Sharifah-Teh, A Saniros, B Hasnita
Intrauterine insemination (IUI) remains a therapeutic option within means of the majority of infertile couples in Malaysia. Therefore additional information on predictors of IUI success in the local context would provide a more concrete basis for counseling patients on expectations and treatment options. A retrospective analysis of 297 couples who underwent 445 IUI cycles from Jan 2005-Mar 2006 was undertaken. Four fifths were Malay with a mean paternal and maternal age of 35.53 +/- 5.82 (range 24-59) and 33...
December 2006: Medical Journal of Malaysia
Martha Merino Ruiz, Hilda Magdalena Luna Aranda, Arturo Morales Martínez, Donato Saldívar Rodríguez, Oscar Vidal Gutiérrez
BACKGROUND: Sperm preparation and acrosomic reaction have been largely studied to explain the physiology of sperm as reproductive cell. OBJECTIVE: To identify the morphological changes on in vitro prepared human sperm cells and in spontaneous and ionophore A23187 induced acrosome reaction of infertile males, through the application of the chlortetracycline test. DESIGN: Experimental prospective study. MATERIALS AND METHODS: Sperm samples of 17 subfertile males were processed as routine for a sperm analysis, we carried out sperm preparation and induction of the acrosomic reaction by means of calcium ions A23187...
May 2004: Ginecología y Obstetricia de México
Aleksandra Kasperczyk, Sławomir Kasperczyk, Maria Dziwisz, Ewa Birkner, Cecylia Walecko, Halina Winiarska, Józef Birkner
OBJECTIVES: The aim of our study was to specify influence of lead and cadmium on semen parameters. MATERIAL AND METHODS: Concentrations of both elements were determined in semen samples by atomic absorption spectrophotometry of 57 men with teratozoospermic, astenozoospermic and oligozoospermic (studied group) and 39 normozoospermic group (control group). Semen morphology was conducted in accordance with WHO regulations. RESULTS AND CONCLUSIONS: We did not find any difference in concentration of these elements in both groups...
May 2002: Ginekologia Polska
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