Sung Wook Hong, Jong-Hui Kim, Hyun A Cha, Kun Sub Chung, Hyo Ju Bae, Won Seo Park, Jun-Sang Ham, Beom-Young Park, Mi-Hwa Oh
Natural antimicrobial substances are needed as alternatives to synthetic antimicrobials to protect against foodborne pathogens. In this study, a bacteriocin-producing bacterium, Bacillus subtilis HD15, was isolated from doenjang, a traditional Korean fermented soybean paste. We sequenced the complete genome of B. subtilis HD15. This genome size was 4,173,431 bp with a G + C content of of 43.58%, 4,305 genes, and 4,222 protein-coding genes with predicted functions, including a subtilosin A gene cluster. The bacteriocin was purified by ammonium sulfate precipitation, Diethylaminoethanol-Sepharose chromatography, and Sephacryl gel filtration, with 12...
October 14, 2022: Journal of Microbiology and Biotechnology