keyword
MENU ▼
Read by QxMD icon Read
search

subtilosin

keyword
https://www.readbyqxmd.com/read/28705677/hyicin-4244-the-first-sactibiotic-described-in-staphylococci-exhibits-an-anti-staphylococcal-biofilm-activity
#1
Andreza Freitas de Souza Duarte, Hilana Ceotto-Vigoder, Emile Santos Barrias, Thaïs Cristina Baeta Soares Souto-Padrón, Ingolf Figved Nes, Maria do Carmo de Freire Bastos
Hyicin 4244 is a small antimicrobial peptide with a broad-spectrum of activity found in the culture supernatant of Staphylococcus hyicus 4244, whose genome was then sequenced. The bacteriocin gene cluster (hyiSABCDEFG) was mined from its single chromosome and exhibited a genetic organization similar to that of subtilosin A. All genes involved in hyicin 4244 biosynthesis proved to be transcribed and encode proteins that share at least 42 % similarity to proteins encoded by the subtilosin A gene cluster. Due to its resemblance to subtilosin A and the presence of three thioether bonds in its structure, hyicin 4244 is assumed to be a 35 amino acid circular sactibiotic, the first to be described in staphylococci...
July 10, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28392780/biocontrol-potentials-of-antimicrobial-peptide-producing-bacillus-species-multifaceted-antagonists-for-the-management-of-stem-rot-of-carnation-caused-by-sclerotinia-sclerotiorum
#2
S Vinodkumar, S Nakkeeran, P Renukadevi, V G Malathi
Bacillus species are widely exploited as biocontrol agents because of their efficiency in impeding various plant pathogens with multifaceted approach. In this study, Bacillus species were isolated from rhizosphere of various plants viz., carnations, cotton, turmeric, and bananas in Tamil Nadu state of India. Their potential to control the mycelial growth of Sclerotinia sclerotiorum was assessed in vitro by dual plate and partition plate techniques. B. amyloliquefaciens strain VB7 was much effective in inhibiting mycelial growth (45% inhibition of over control) and sclerotial production (100%)...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28197131/bacillus-spp-isolated-from-puba-as-a-source-of-biosurfactants-and-antimicrobial-lipopeptides
#3
Karla J Perez, Jaime Dos Santos Viana, Fernanda C Lopes, Jamile Q Pereira, Daniel M Dos Santos, Jamil S Oliveira, Renata V Velho, Silvia M Crispim, Jacques R Nicoli, Adriano Brandelli, Regina M D Nardi
Several products of industrial interest are produced by Bacillus, including enzymes, antibiotics, amino acids, insecticides, biosurfactants and bacteriocins. This study aimed to investigate the potential of two bacterial isolates (P5 and C3) from puba, a regional fermentation product from cassava, to produce multiple substances with antimicrobial and surface active properties. Phylogenetic analyses showed close relation of isolates P5 and C3 with Bacillus amyloliquefaciens and Bacillus thuringiensis, respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27990138/purification-of-antilisterial-peptide-subtilosin-a-from-novel-bacillus-tequilensis-fr9-and-demonstrate-their-pathogen-invasion-protection-ability-using-human-carcinoma-cell-line
#4
Rizwana Parveen Rani, Marimuthu Anandharaj, Subramani Hema, Ramasamy Deepika, Abraham David Ravindran
This study focuses on isolation, screening, and characterization of novel probiotics from gastrointestinal tract of free-range chicken (Gallus gallus domesticus). Fifty seven colonies were isolated and three isolates (FR4, FR9, and FR12) were selected and identified as Lactobacillus gasseri FR4, Bacillus tequilensis FR9, and L. animalis FR12 by 16S rRNA sequencing. Three strains were able to survive in stimulated acidic and bile conditions and inhibit the growth of pathogens. Especially, FR9 exhibited maximum inhibition against Listeria monocytogenes and none of them exhibited hemolytic activity...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27914001/subtilosin-prevents-biofilm-formation-by-inhibiting-bacterial-quorum-sensing
#5
Ammar Algburi, Saskia Zehm, Victoria Netrebov, Anzhelica B Bren, Vladimir Chistyakov, Michael L Chikindas
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27184432/complete-genome-sequence-of-bacillus-subtilis-bsd-2-a-microbial-germicide-isolated-from-cultivated-cotton
#6
Hongwei Liu, Shuli Yin, Likang An, Genwei Zhang, Huicai Cheng, Yanhua Xi, Guanhui Cui, Feiyan Zhang, Liping Zhang
Bacillus subtilis BSD-2, isolated from cotton (Gossypium spp.), had strong antagonistic activity to Verticillium dahlia Kleb and Botrytis cinerea. We sequenced and annotated the BSD-2 complete genome to help us the better use of this strain, which has surfactin, bacilysin, bacillibactin, subtilosin A, Tas A and a potential class IV lanthipeptide biosynthetic pathways.
July 20, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27087315/thioether-bond-formation-by-spasm-domain-radical-sam-enzymes-c%C3%AE-h-atom-abstraction-in-subtilosin-a-biosynthesis
#7
Alhosna Benjdia, Alain Guillot, Benjamin Lefranc, Hubert Vaudry, Jérôme Leprince, Olivier Berteau
AlbA is a radical SAM enzyme catalyzing the formation of three unusual thioether bonds in the antibiotic subtilosin A. We demonstrate here that AlbA catalyzes direct Cα H-atom abstraction and likely contains three essential [4Fe-4S] centers. This leads us to propose novel mechanistic perspectives for thioether bond catalysis by radical SAM enzymes.
May 7, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27019323/production-of-sactipeptides-in-escherichia-coli-probing-the-substrate-promiscuity-of-subtilosin-a-biosynthesis
#8
Paul M Himes, Scott E Allen, Sungwon Hwang, Albert A Bowers
Sactipeptides are peptide-derived natural products that are processed by remarkable, radical-mediated cysteine sulfur to α-carbon coupling reactions. The resulting sactionine thioether linkages give rise to the unique defined structures and concomitant biological activities of sactipeptides. An E. coli heterologous expression system, based on the biosynthesis of one such sactipeptide, subtilosin A, is described and this expression system is exploited to probe the promiscuity of the subtilosin A sactionine bond-forming enzyme, AlbA...
June 17, 2016: ACS Chemical Biology
https://www.readbyqxmd.com/read/26648120/application-of-bacterial-cytological-profiling-to-crude-natural-product-extracts-reveals-the-antibacterial-arsenal-of-bacillus-subtilis
#9
Poochit Nonejuie, Rachelle M Trial, Gerald L Newton, Anne Lamsa, Varahenage Ranmali Perera, Julieta Aguilar, Wei-Ting Liu, Pieter C Dorrestein, Joe Pogliano, Kit Pogliano
Although most clinically used antibiotics are derived from natural products, identifying new antibacterial molecules from natural product extracts is difficult due to the complexity of these extracts and the limited tools to correlate biological activity with specific molecules. Here, we show that bacterial cytological profiling (BCP) provides a rapid method for mechanism of action determination on plates and in complex natural product extracts and for activity-guided purification. We prepared an extract from Bacillus subtilis 3610 that killed the Escherichia coli lptD mutant and used BCP to observe two types of bioactivities in the unfractionated extract: inhibition of translation and permeablization of the cytoplasmic membrane...
May 2016: Journal of Antibiotics
https://www.readbyqxmd.com/read/26031307/the-sactibiotic-subclass-of-bacteriocins-an-update
#10
REVIEW
Harsh Mathur, Mary C Rea, Paul D Cotter, Colin Hill, R Paul Ross
The sactibiotics are a recently designated subclass of bacteriocins that contain characteristic cysteine sulphur to α -carbon linkages mediated through post-translational modifications. They are a relatively small subclass of bacteriocins compared to the most thoroughly studied lantibiotics. The sactibiotics that have been extensively studied thus far are thuricin CD, subtilosin A, thurincin H, and propionicin F. Despite their recent discovery, there have already been significant advances made in the study of sactibiotics, most notably the discovery of the narrow spectrum anti-Clostridium difficile sactibiotic, thuricin CD...
2015: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/26017225/properties-of-a-bacteriocin-produced-by-bacillus-subtilis-emd4-isolated-from-ganjang-soy-sauce
#11
Xiaoming Liu, Jae Yong Lee, Seon-Ju Jeong, Kye Man Cho, Gyoung Min Kim, Jung-Hye Shin, Jong-Sang Kim, Jeong Hwan Kim
A Bacillus species, EMD4, with strong antibacterial activity was isolated from ganjang (soy sauce) and identified as B. subtilis. B. subtilis EMD4 strongly inhibited the growth of B. cereus ATCC14579 and B. thuringiensis ATCC33679. The antibacterial activity was stable at pH 3-9 but inactive at pH 10 and above. The activity was fully retained after 15 min at 80°C but reduced by 50% after 15 min at 90°C. The activity was completely destroyed by proteinase K and protease treatment, indicating its proteinaceous nature...
September 2015: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/25838136/natural-antimicrobials-subtilosin-and-lauramide-arginine-ethyl-ester-synergize-with-conventional-antibiotics-clindamycin-and-metronidazole-against-biofilms-of-gardnerella-vaginalis-but-not-against-biofilms-of-healthy-vaginal-lactobacilli
#12
Ammar Algburi, Anna Volski, Michael L Chikindas
The purpose of this study was to evaluate the ability of clindamycin and metronidazole to synergize with natural antimicrobials against biofilms of bacterial vaginosis (BV)-associated Gardnerella vaginalis. Minimum bactericidal concentrations for biofilm cells (MBCs-B) were determined for each antimicrobial. The MBCs-B of lauramide arginine ethyl ester (LAE), subtilosin, clindamycin and metronidazole were 50, 69.5, 20 and 500 μg mL(-1), respectively. A checkerboard assay and isobologram were used to analyze the type of interactions between these antimicrobials...
July 2015: Pathogens and Disease
https://www.readbyqxmd.com/read/25817994/pqqd-is-a-novel-peptide-chaperone-that-forms-a-ternary-complex-with-the-radical-s-adenosylmethionine-protein-pqqe-in-the-pyrroloquinoline-quinone-biosynthetic-pathway
#13
John A Latham, Anthony T Iavarone, Ian Barr, Prerak V Juthani, Judith P Klinman
Pyrroloquinoline quinone (PQQ) is a product of a ribosomally synthesized and post-translationally modified pathway consisting of five conserved genes, pqqA-E. PqqE is a radical S-adenosylmethionine (RS) protein with a C-terminal SPASM domain, and is proposed to catalyze the formation of a carbon-carbon bond between the glutamate and tyrosine side chains of the peptide substrate PqqA. PqqD is a 10-kDa protein with an unknown function, but is essential for PQQ production. Recently, in Klebsiella pneumoniae (Kp), PqqD and PqqE were shown to interact; however, the stoichiometry and KD were not obtained...
May 15, 2015: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/25588687/the-natural-antimicrobial-subtilosin-a-synergizes-with-lauramide-arginine-ethyl-ester-lae-%C3%AE%C2%B5-poly-l-lysine-polylysine-clindamycin-phosphate-and-metronidazole-against-the-vaginal-pathogen-gardnerella-vaginalis
#14
Veronica L Cavera, Anna Volski, Michael L Chikindas
Bacterial vaginosis (BV) is a common, recurrent vaginal infection linked to increased chances of preterm delivery, incidence of sexually transmitted infections and fertility problems. BV is caused by a shift of the vaginal ecosystem from predominately Lactobacillus to a multispecies Actinomyces biofilm with the most common representatives identified as Gardnerella vaginalis and Prevotella spp. Current treatments have been associated with increased resistance as well as negative effects on healthy microbiota...
June 2015: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/25524109/bacillus-subtilis-biosensor-engineered-to-assess-meat-spoilage
#15
Alicja Daszczuk, Yonathan Dessalegne, Ismaêl Drenth, Elbrich Hendriks, Emeraldo Jo, Tom van Lente, Arjan Oldebesten, Jonathon Parrish, Wlada Poljakova, Annisa A Purwanto, Renske van Raaphorst, Mirjam Boonstra, Auke van Heel, Martijn Herber, Sjoerd van der Meulen, Jeroen Siebring, Robin A Sorg, Matthias Heinemann, Oscar P Kuipers, Jan-Willem Veening
Here, we developed a cell-based biosensor that can assess meat freshness using the Gram-positive model bacterium Bacillus subtilis as a chassis. Using transcriptome analysis, we identified promoters that are specifically activated by volatiles released from spoiled meat. The most strongly activated promoter was PsboA, which drives expression of the genes required for the bacteriocin subtilosin. Next, we created a novel BioBrick compatible integration plasmid for B. subtilis and cloned PsboA as a BioBrick in front of the gene encoding the chromoprotein amilGFP inside this vector...
December 19, 2014: ACS Synthetic Biology
https://www.readbyqxmd.com/read/25440998/antibacterial-activity-and-genotypic-phenotypic-characteristics-of-bacteriocin-producing-bacillus-subtilis-kku213-potential-as-a-probiotic-strain
#16
Nalisa Khochamit, Surasak Siripornadulsil, Peerapol Sukon, Wilailak Siripornadulsil
The antimicrobial activity and probiotic properties of Bacillus subtilis strain KKU213, isolated from local soil, were investigated. The cell-free supernatant (CFS) of a KKU213 culture containing crude bacteriocins exhibited inhibitory effects on Gram-positive bacteria, including Bacillus cereus, Listeria monocytogenes, Micrococcus luteus, and Staphylococcus aureus. The antibacterial activity of the CFS precipitated with 40% ammonium sulfate (AS) remained even after treatment at 60 and 100 °C, at pH 4 and 10 and with proteolytic enzymes, detergents and heavy metals...
January 2015: Microbiological Research
https://www.readbyqxmd.com/read/25087911/antiherpes-simplex-virus-type-2-activity-of-the-antimicrobial-peptide-subtilosin
#17
V M Quintana, N I Torres, M B Wachsman, P J Sinko, V Castilla, M Chikindas
AIMS: In this study, we evaluated the antiviral activity of subtilosin, a cyclical peptide isolated from Bacillus amyloliquefaciens, against herpes simplex virus type 2 (HSV-2) in cell cultures and we investigated subtilosin mode of action. METHODS AND RESULTS: We determined, using a virus yield inhibition assay, that noncytotoxic concentrations of subtilosin inhibit HSV-2 replication in Vero cell cultures. Subtilosin strongly inhibited extracellular and total virus production even when it was added at 8 h postinfection indicating that not only virus release but also viral particle formation is impeded by the antiviral peptide...
November 2014: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/24948771/draft-genome-sequence-of-bacillus-subtilis-strain-katmira1933
#18
Andrey V Karlyshev, Vyacheslav G Melnikov, Michael L Chikindas
In this report, we present a draft sequence of Bacillus subtilis KATMIRA1933. Previous studies demonstrated probiotic properties of this strain partially attributed to production of an antibacterial compound, subtilosin. Comparative analysis of this strain's genome with that of a commercial probiotic strain, B. subtilis Natto, is presented.
2014: Genome Announcements
https://www.readbyqxmd.com/read/24566190/polyethylene-glycol-based-hydrogels-for-controlled-release-of-the-antimicrobial-subtilosin-for-prophylaxis-of-bacterial-vaginosis
#19
Sujata Sundara Rajan, Veronica L Cavera, Xiaoping Zhang, Yashveer Singh, Michael L Chikindas, Patrick J Sinko
Current treatment options for bacterial vaginosis (BV) have been shown to be inadequate at preventing recurrence and do not provide protection against associated infections, such as that with HIV. This study examines the feasibility of incorporating the antimicrobial peptide subtilosin within covalently cross-linked polyethylene glycol (PEG)-based hydrogels for vaginal administration. The PEG-based hydrogels (4% and 6% [wt/vol]) provided a two-phase release of subtilosin, with an initial rapid release rate of 4...
May 2014: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/24104687/rna-seq-analysis-of-antibiotic-producing-bacillus-subtilis-sc-8-in-response-to-signal-peptide-papr-of-bacillus-cereus
#20
In-Cheol Yeo, Nam Keun Lee, Byung Wook Yang, Young Tae Hahm
Bacillus subtilis SC-8 produces an antibiotic that has narrow antagonistic activity against bacteria in the Bacillus cereus group. In B. cereus group bacteria, peptide-activating PlcR (PapR) plays a significant role in regulating the transcription of virulence factors. When B. subtilis SC-8 and B. cereus are co-cultured, PapR is assumed to stimulate antibiotic production by B. subtilis SC-8. To better understand the effect of PapR on this interspecies interaction, the global transcriptome profile of B. subtilis SC-8 was analyzed in the presence of PapR...
January 2014: Applied Biochemistry and Biotechnology
keyword
keyword
73061
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"