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https://www.readbyqxmd.com/read/28547889/comparison-between-chaotropic-and-detergent-based-sample-preparation-workflow-in-tendon-for-mass-spectrometry-analysis
#1
Yalda A Kharaz, Danae Zamboulis, Karen Sander, Eithne Comerford, Peter Clegg, Mandy Peffers
Exploring the tendon proteome is a challenging but important task for understanding the mechanisms of physiological/pathological processes during ageing and disease and for the development of new treatments. Several extraction methods have been utilised for tendon mass spectrometry, however different extraction methods have not been simultaneously compared. In the present study we compared protein extraction in tendon with two chaotropic agents, guanidine hydrochloride (GnHCl) and urea, a detergent, RapiGest(ۛ) , and their combinations for shotgun mass spectrometry...
May 26, 2017: Proteomics
https://www.readbyqxmd.com/read/28544170/hamstring-injury-prevention-in-soccer-before-or-after-training
#2
Ric Lovell, Michael Knox, Matthew Weston, Jason C Siegler, Scott Brennan, Paul W M Marshall
We examined the effects of a 12-week program of Nordic hamstring exercises (NHE), administered before or after football training, upon eccentric hamstring strength, muscle activity, and architectural adaptations. Amateur soccer players were randomized into 3 groups. The control group (CON; n=11) undertook core stability exercises, whereas a periodized NHE program was delivered either before (NHEBEF ; n=10) or after (NHEAFT ; n=14) bi-weekly training sessions. Outcome measures included peak torque and concomitant normalized peak surface electromyography signals (sEMG) of the biceps femoris (BF) and medial hamstring (MH) muscles during knee flexor maximal eccentric contractions, performed at 30°·s(-1) ...
May 24, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28544029/learned-control-over-spinal-nociception-in-patients-with-chronic-back-pain
#3
S Krafft, H-D Göhmann, J Sommer, A Straube, R Ruscheweyh
BACKGROUND: Descending pain inhibition suppresses spinal nociception, reducing nociceptive input to the brain. It is modulated by cognitive and emotional processes. In subjects with chronic pain, it is impaired, possibly contributing to pain persistence. A previously developed feedback method trains subjects to activate their descending inhibition. Participants are trained to use cognitive-emotional strategies to reduce their spinal nociception, as quantified by the nociceptive flexor reflex (RIII reflex), under visual feedback about their RIII reflex size...
May 24, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28543432/clinically-amyopathic-dermatomyositis-with-rapidly-progressive-interstitial-pneumonia-the-relation-between-the-disease-activity-and-the-serum-interleukin-6-level
#4
Hazuki Yasuda, Takaharu Ikeda, Yasuhito Hamaguchi, Fukumi Furukawa
A 67-year-old woman exhibited chilblain-like erythema on the bilateral auricles, erythema with hyperkeratosis on the sides of fingers and the bilateral elbows, and erythema on the extensor/flexor sides of the finger joints and the iliac regions. Fine crackles were audible on the dorsal side of the bilateral lower lung regions. We diagnosed clinically amyopathic dermatomyositis accompanied by rapidly progressive interstitial pneumonia. While we initiated immunosuppressive therapy, the respiratory failure showed no responses...
May 23, 2017: Journal of Dermatology
https://www.readbyqxmd.com/read/28542291/the-first-darter-aves-anhingidae-fossils-from-india-late-pliocene
#5
Thomas Stidham, Rajeev Patnaik, Kewal Krishan, Bahadur Singh, Abhik Ghosh, Ankita Singla, Simran S Kotla
New fossils from the latest Pliocene portion of the Tatrot Formation exposed in the Siwalik Hills of northern India represent the first fossil record of a darter (Anhingidae) from India. The darter fossils possibly represent a new species, but the limited information on the fossil record of this group restricts their taxonomic allocation. The Pliocene darter has a deep pit on the distal face of metatarsal trochlea IV not reported in other anhingids, it has an open groove for the m. flexor perforatus et perforans digiti II tendon on the hypotarsus unlike New World anhingid taxa, and these darter specimens are the youngest of the handful of Neogene records of the group from Asia...
2017: PloS One
https://www.readbyqxmd.com/read/28538572/intratendinous-injection-of-hydrogel-for-reseeding-decellularized-human-flexor-tendons
#6
Chao Long, Michael G Galvez, Anais Legrand, Lydia-Marie Joubert, Zhen Wang, Arhana Chattopadhyay, James Chang, Paige M Fox
BACKGROUND: Decellularized cadaveric tendons are a potential source for reconstruction. Reseeding to enhance healing is ideal; however, cells placed on the tendon surface result in inadequate delivery. The authors used an injection technique to evaluate intratendinous cell delivery. METHODS: Decellularized tendons were reseeded with adipose-derived stem cells in culture, and injected with fetal bovine serum or hydrogel. PKH26-stained cells in cross-section were quantified...
June 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28538571/angiosome-based-allografts-vascularized-composite-allotransplantation-for-tailored-subunit-reconstruction-with-volkmann-ischemic-contracture-as-a-case-in-point
#7
G Ian Taylor, David S Sparks, Adam C Gascoigne, Russell J Corlett, Mark W Ashton
BACKGROUND: As we enter an age with new approaches to tissue reconstruction, the emphasis on the adage "like for like" has become even more relevant. This study illustrates the potential for several tailored vascularized composite allotransplantation reconstructive techniques and, in particular, for the management of Volkmann contracture. METHODS: Twenty fresh cadaver dissections and 30 archival lead oxide radiographic studies were examined to (1) identify potential upper limb vascularized composite allotransplantation donor sites (i...
June 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28533851/-giant-cell-tumors-of-the-tendon-sheaths-of-the-hand-about-50-cases
#8
Walid Osman, Zeineb Alaya, Ali Haggui, Mohamed Ben Rejeb, Sonia Jemni, Nader Naouar, Mohamed Laziz Ben Ayeche
Giant cell tumors of the synovial tendon sheaths (GCTSTS) are a localized form of hemopigmented villonodular synovitis. They mainly affect the hands. This study aims to analyse the epidemiology, clinical and therapeutic characteristics of GCTSTS, to assess the results of surgical treatment and to identify risk factors for recurrences. We conducted a retrospective data collection from medical records of 50 patients with GCTSTS of the hand between 1992 and 2016 in the Department of Orthopaedics at the Hospital of Sahloul (Sousse-Tunisia)...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/28533627/reduced-foot-pain-after-spasticity-control-with-alcohol-block-in-a-patient-with-chronic-hemiparetic-stroke-a-case-report
#9
Min Cheol Chang
[Purpose] This study report a case of a patient with hemiparetic stroke who showed significantly reduced foot pain when ankle spasticity was reduced using nerve and motor point blocks with 20% ethyl alcohol. [Subject and Methods] A 58-year-old woman with left hemiparesis following intracranial hemorrhage five years previously presented with pain in the left fifth metatarsal head for two years (numeric rating scale[NRS]: 8). Erythema and edema were observed on the lateral aspect of the head of the fifth metatarsal bone...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28533626/nonhealing-progressive-stress-fractures-of-the-foot-in-a-13-year-old-basketball-player-is-vitamin-k-deficiency-a-risk-factor
#10
Meral Bayramoğlu, Nuray Ünlütürk
[Purpose] To report an adolescent male basketball player with nonhealing stress fractures of the foot and discuss the probable factors. [Subject and Methods] A 13-year-old basketball player presented with right foot pain. He had been playing basketball for three years and practicing 5 days/week. He denied any increase in daily training intensity. Magnetic resonance imaging confirmed stress fractures of the cuboid and cuneiform, with mild edema of the soft tissues between the tarsal bones and tenosynovitis of the flexor hallucis and flexor digitorum longi...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28533606/the-relationship-between-knee-joint-angle-and-knee-flexor-and-extensor-muscle-strength
#11
Misook Ha, Dongwook Han
[Purpose] The aim of this study was to determine a relationship between joint angle and muscular strength. In particular, this research investigated the differences in maximum muscular strength and average muscular strength at the knee-joint posture. [Subjects and Methods] The study subjects comprised eight female students in their 20s attending S University in Busan. None of the subjects had functional disabilities or had experienced damage to the lower extremities in terms of measurement of muscular strength...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28533593/effects-of-repeated-vibratory-stimulation-of-wrist-and-elbow-flexors-on-hand-dexterity-strength-and-sensory-function-in-patients-with-chronic-stroke-a-pilot-study
#12
Won-Ho Choi
[Purpose] The aim of this study was to investigate the effects of repeated vibratory stimulation to muscles related to hand functions on dexterity, strength, and sensory function in patients with chronic stroke. [Subjects and Methods] A total of 10 stroke patients with hemiplegia participated in this study. They were divided into two groups: a) Experimental and b) Control, with five randomly selected subjects for each group. The experimental group received vibratory stimulation, while the control group received the traditional physical therapy...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28533587/measuring-hip-flexor-and-extensor-strengths-across-various-postures-using-a-fixed-belt
#13
Takashi Yoshizawa, Kentarou Higashi, Tomohiko Katou
[Purpose] The purpose of this study was to evaluate hip flexor and extensor strength due to differences in posture measurement. [Subjects and Methods] Healthy adults (26 males and 24 females) were enrolled. Using a hand-held dynamometer, hip flexor and extensor strengths were measured in various postures and in a basic posture (sitting posture and hip/knee joint flexed 90°) according to the Manual Muscle Testing procedure. While calculating intraclass correlation coefficients by measurement, hip flexor and extensor strengths were compared in males and females for every posture...
April 2017: Journal of Physical Therapy Science
https://www.readbyqxmd.com/read/28532877/task-based-mirror-therapy-enhances-ipsilesional-motor-functions-in-stroke-a-pilot-study
#14
Kamal Narayan Arya, Shanta Pandian, Dharmendra Kumar
OBJECTIVE: To examine the effect of Mirror therapy (MT) on dexterity, coordination, and muscle strength of the less-affected upper limb in stroke. DESIGN: Pre-test post-test, single group, experimental design. SETTING: Rehabilitation institute. PARTICIPANTS: Post-stroke hemiparetic chronic subjects (N = 21). INTERVENTIONS: Forty sessions of MT using various tasks in addition to the conventional rehabilitation...
April 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/28532868/the-local-and-referred-pain-patterns-of-the-longus-colli-muscle
#15
Amir Minerbi, Motti Ratmansky, Aharon Finestone, Robert Gerwin, Simon Vulfsons
The longus colli muscle is a neck flexor believed to play an important role in pain originating in the neck region, including pain resulting from whiplash injuries. Despite the clinical importance attributed to it, the pain referral pattern of the longus colli has previously been described only in a small cohort of subjects. Here, we aim to delineate the pain referral pattern of the longus colli muscle. Thirty-five healthy volunteers underwent deep massage of the longus colli followed by dry needling of the muscle...
April 2017: Journal of Bodywork and Movement Therapies
https://www.readbyqxmd.com/read/28532279/the-effect-of-cold-ambient-temperatures-on-climbing-specific-finger-flexor-performance
#16
Kevin Phillips, Byungjoo Noh, Matthew Gage, Tejin Yoon
Different ambient temperatures are known to affect muscular performance based on the type of contraction. The effect of cold (10°C) and thermoneutral (TN) (24°C) ambient temperatures on finger flexor performance was examined in 12 rock climbers. After 30 min of seated rest in the designated temperature condition, participants completed maximal voluntary contractions (MVC) on a climbing-specific finger flexor assessment device equipped with a crimp grip hold. Participants then completed an intermittent fatiguing task until failure...
May 22, 2017: European Journal of Sport Science
https://www.readbyqxmd.com/read/28532248/effects-of-drop-sets-with-resistance-training-on-increases-in-muscle-csa-strength-and-endurance-a-pilot-study
#17
Hayao Ozaki, Atsushi Kubota, Toshiharu Natsume, Jeremy P Loenneke, Takashi Abe, Shuichi Machida, Hisashi Naito
To investigate the effects of a single high-load (80% of one repetition maximum [1RM]) set with additional drop sets descending to a low-load (30% 1RM) without recovery intervals on muscle strength, endurance, and size in untrained young men. Nine untrained young men performed dumbbell curls to concentric failure 2-3 days per week for 8 weeks. Each arm was randomly assigned to one of the following three conditions: 3 sets of high-load (HL, 80% 1RM) resistance exercise, 3 sets of low-load [LL, 30% 1RM] resistance exercise, and a single high-load (SDS) set with additional drop sets descending to a low-load...
May 22, 2017: Journal of Sports Sciences
https://www.readbyqxmd.com/read/28530766/right-ring-finger-volar-mass-in-a-14-year-old-boy
#18
Mary P Fox, Jack E McKay, Randall D Craver, Nicholas D Pappas
A trigger digit is relatively uncommon in adolescents and often has a different etiology in that age group vs adults. In the pediatric population, trigger digits frequently arise from a variety of underlying anatomic situations, including thickening of the flexor digitorum superficialis or flexor digitorum profundus tendons, an abnormal relationship between the flexor digitorum superficialis and flexor digitorum profundus tendons, a proximal flexor digitorum superficialis decussation, or constriction of the pulleys...
May 20, 2017: Orthopedics
https://www.readbyqxmd.com/read/28530482/the-effect-of-backpack-load-carriage-on-the-kinetics-and-kinematics-of-ankle-and-knee-joints-during-uphill-walking
#19
Jinkyu Lee, Yong-Jin Yoon, Choongsoo S Shin
The purpose of this study was to investigate the effect of load carriage on the kinematics and kinetics of the ankle and knee joints during uphill walking, including joint work, range of motion (ROM), and stance time. Fourteen males walked at a self-selected speed on an uphill (15°) slope wearing military boots and carrying a rifle in hand without a backpack (control condition) and with a backpack. The results showed that the stance time significantly decreased with backpack carriage (p<.05). The mediolateral impulse significantly increased with backpack carriage (p<...
May 22, 2017: Journal of Applied Biomechanics
https://www.readbyqxmd.com/read/28527751/hand-strengthening-exercises-in-chronic-stroke-patients-dose-response-evaluation-using-electromyography
#20
Jonas Vinstrup, Joaquin Calatayud, Markus D Jakobsen, Emil Sundstrup, Jørgen R Jørgensen, Jose Casaña, Lars L Andersen
STUDY DESIGN: Cross-sectional. PURPOSE OF THE STUDY: This study evaluates finger flexion and extension strengthening exercises using elastic resistance in chronic stroke patients. METHODS: Eighteen stroke patients (mean age: 56.8 ± 7.6 years) with hemiparesis performed 3 consecutive repetitions of finger flexion and extension, using 3 different elastic resistance levels (easy, moderate, and hard). Surface electromyography was recorded from the flexor digitorum superficialis (FDS) and extensor digitorum (ED) muscles and normalized to the maximal electromyography of the non-paretic arm...
May 17, 2017: Journal of Hand Therapy: Official Journal of the American Society of Hand Therapists
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