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https://www.readbyqxmd.com/read/29338387/quantifying-the-effects-of-different-neutral-proteases-on-human-islet-integrity
#1
Heide Brandhorst, Paul R V Johnson, Olle Korsgren, Daniel Brandhorst
Efficient islet release from the pancreas requires the combination of collagenase, neutral protease (cNP), or thermolysin (TL). Recently, it has been shown that clostripain (CP) may also contribute to efficient islet release from the human pancreas. The aim of this study was to evaluate the impact of these proteases on human islet integrity in a prospective approach. Islets were isolated from the pancreas of 10 brain-dead human organ donors. Purified islets were precultured for 3 to 4 d at 37 °C to ensure that preparations were cleared of predamaged islets, and only integral islets were subjected to 90 min of incubation at 37 °C in Hank's balanced salt solution supplemented with cNP, TL, or CP...
November 2017: Cell Transplantation
https://www.readbyqxmd.com/read/29278861/hemitransdomal-versus-dome-binding-suture
#2
Fernando Casanueva, Deniz Gerecci, Felipe Cardemil
The dome-binding suture (DBS) and hemitransdomal suture (HTS) are suture techniques used to narrow and define the nasal tip. The DBS can create a pinched, unnatural appearance, while the HTS puts the lateral crus in a more favorable orientation. This allows a natural contour between the nasal tip and alar lobule while maintaining alar margin support. Objective measurement of the rotational axis of the lateral crus between the DBS and the HTS has not been reported in the literature. To determine whether the DBS or HTS technique results in a more favorable rotational axis of the lateral crus as measured by the alar surface septal angle (ASSA)...
December 26, 2017: Facial Plastic Surgery: FPS
https://www.readbyqxmd.com/read/29225091/gene-expression-profiling-of-the-trim-protein-family-reveals-potential-biomarkers-for-indicating-tuberculosis-status
#3
Yanqing Chen, Shuhui Cao, Yong Sun, Chuanyou Li
Tripartite motif (TRIM) family proteins play important regulatory roles in innate immune responses, the dysregulation of which cause several infectious diseases. However, the role and function of TRIM family proteins during tuberculosis (TB) infection remains unclear. In this study, we employed real-time quantitative PCR to profile the transcript levels of 72 TRIM genes from a cohort of 5 active TB patients, 5 latent tuberculosis infection (LTBI) subjects, and 5 healthy controls (HCs) in an initial discovery phase...
December 7, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/29223628/five-and-10-year-follow-up-of-nonvascularized-toe-phalanx-transfers
#4
Hidehiko Kawabata, Daisuke Tamura
PURPOSE: The purpose of this study was to evaluate long-term outcomes of nonvascularized toe phalanx transfer. METHODS: We retrospectively reviewed 54 nonvascularized toe phalanx transfers in 29 children with symbrachydactyly. Forty-seven transfers in 24 children were evaluated at 5-year follow-up and 27 transfers in 14 children were evaluated at 10-year follow-up. We recorded the incidence of the early physeal closure and the length of the transferred toe phalanx on plain radiographs at 5- and 10-year follow-up...
December 6, 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/29172722/effect-of-switch-trimming-on-udder-and-teat-hygiene-of-dairy-cows
#5
Heather D Ingle, Christa A Rice, Randi A Black, Sarah Z Childers, Nicole L Eberhart, Maria E Prado, Peter D Krawczel
The study objective was to determine the effects of trimming the switch of dairy cows on teat-end bacterial counts and udder hygiene scores. Cows (n = 102) were blocked by days in milk, milk production, and parity and then assigned to (a) treatment (trimming of their tail switch using a commercially available trimmer), or (b) control (unaltered tails). Udder hygiene was recorded for cows on Days 0 (initiation of treatment), 32, and 64. A subset of cows (n = 21) was used to assess Streptococci and coliform bacterial populations on teat ends...
November 27, 2017: Journal of Applied Animal Welfare Science: JAAWS
https://www.readbyqxmd.com/read/29166174/low-prevalence-of-salmonella-and-shiga-toxin-producing-escherichia-coli-in-lymph-nodes-of-australian-beef-cattle
#6
Gavin Bailey, Long Huynh, Lachlan Govenlock, David Jordan, Ian Jenson
Salmonella contamination of ground beef has been viewed as originating from the surface of carcasses. Recent studies have identified lymph nodes as a potential source of Salmonella contamination because these tissues play an active role in containment of pathogens in the live animal and because some lymph nodes are unavoidably present in manufacturing beef trimmings or primal cuts that may be incorporated into ground beef. A survey was conducted of the microbiological status of lymph nodes from Australian cattle at the time of slaughter to determine the prevalence of microbiological contamination...
November 22, 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/29152618/effect-of-mechanical-fatigue-on-the-bond-between-zirconia-and-composite-cement
#7
Hugo A Vidotti, Ricardo M Carvalho, Paulo G Coelho, Willian F Zambuzzi, Gerson Bonfante, Accácio L do Valle, Estevam A Bonfante
PURPOSE: To examine the effect of mechanical fatigue on the bond strength of resin composite cemented to silicacoated yttria-tetragonal zirconia polycrystal ceramic (Y-TZP). MATERIALS AND METHODS: Ten Y-TZP blocks were polished down to 600-grit silicon carbide paper. Specimens were silica coated by airborne-particle abrasion with 30-μm silica-modified Al2O3 particles. Blocks were cleaned in an ultrasonic bath, and a dental adhesive was applied and light cured for 20 s...
November 17, 2017: Journal of Adhesive Dentistry
https://www.readbyqxmd.com/read/29147886/peromyscus-leucopus-mouse-brain-transcriptome-response-to-powassan-virus-infection
#8
Luwanika Mlera, Kimberly Meade-White, Eric Dahlstrom, Rachel Baur, Kishore Kanakabandi, Kimmo Virtaneva, Stephen F Porcella, Marshall E Bloom
Powassan virus (POWV) is a tick-borne Flavivirus responsible for life-threatening encephalitis in North America and some regions of Russia. The ticks that have been reported to transmit the virus belong to the Ixodes species, and they feed on small-to-medium-sized mammals, such as Peromyscus leucopus mice, skunks, and woodchucks. We previously developed a P. leucopus mouse model of POWV infection, and the model is characterized by a lack of clinical signs of disease following intraperitoneal or intracranial inoculation...
November 16, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/29147561/synthesis-and-reactivity-of-a-4his-enzyme-model-complex
#9
Jia Li, Atanu Banerjee, Timothy A Hasse, Reza Loloee, Shannon M Biros, Richard J Staples, Ferman A Chavez
A new iron(II) complex has been prepared and characterized. [Fe(TrIm)4(OTf)2] (1, TrIm = 1-Tritylimidazole). The solid state structure of 1 has been determined by X-ray crystallography. Compound 1 crystallizes in triclinic space group P1̄, with a = 13.342(7) Å, b = 13.5131(7) Å and c = 13.7025(7) Å. The iron center resides in distorted octahedral geometry coordinated to four equatorial imidazole groups and two axial triflate oxygens groups. The complex is high spin between 20 K and 300 K as indicated by variable field variable temperature magnetic measurements...
2017: RSC Advances
https://www.readbyqxmd.com/read/28977524/regional-specific-modulation-of-stress-induced-neuronal-activation-associated-with-the-psd95-nos-interaction-inhibitor-zl006-in-the-wistar-kyoto-rat
#10
Eoin Sherwin, Aifric Lennon, Andrew Harkin
Background: To determine brain areas involved in the antidepressant-related behavioral effects of the selective neuronal nitric oxide synthase inhibitor 1-(2-Trifluoro-methyl-phenyl) imidazole (TRIM) and experimental test compound 4-((3,5-dichloro-2-hydroxybenzyl)amino)-2-hydroxybenzoic acid (ZL006), an inhibitor of the PSD of 95 kDa/neuronal nitric oxide synthase interaction in the N-methyl-D-aspartic acid receptor signalling pathway, regional specific expression of the neuronal activation marker c-FOS was assessed following exposure to the forced swimming test in the Wistar Kyoto rat...
October 1, 2017: International Journal of Neuropsychopharmacology
https://www.readbyqxmd.com/read/28968135/comparison-of-clinical-and-radiographic-outcomes-between-central-and-lateral-lesions-after-osteochondral-autograft-transplantation-for-osteochondritis-dissecans-of-the-humeral-capitellum
#11
Takeshi Matsuura, Yusuke Hashimoto, Kazuya Nishino, Yohei Nishida, Shinji Takahashi, Nagakazu Shimada
BACKGROUND: Clinical studies have reported satisfactory results after osteochondral autograft transplantation (OAT) for central lesions of unstable osteochondritis dissecans (OCD) of the elbow. However, the outcomes after OAT for lateral lesions remain unclear. HYPOTHESIS: The clinical outcomes of OAT would be better for central lesions than for lateral lesions. STUDY DESIGN: Cohort study; Level of evidence, 3. METHODS: The authors retrospectively evaluated 103 juvenile athletes (mean age, 13...
September 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28933219/suppression-of-trim32-enhances-motor-function-repair-after-traumatic-brain-injury-associated-with-antiapoptosis
#12
Zi-Bin Zhang, Liu-Lin Xiong, Bin-Tuan Lu, Hui-Xiang Zhang, Piao Zhang, Ting-Hua Wang
To investigate the role of Trim32 in traumatic brain injury (TBI), adult male Sprague Dawley (SD) rats and mice were randomly divided into sham (n = 6) and TBI groups ( n = 24), respectively. Then, mice were assigned into Trim32 knockout mice (Trim32-KO [+/-]) and wild-type (WT) littermates. The TBI model used was the Feeney free-falling model, and neurological function was evaluated after TBI using a neurological severity score (NSS). Reverse transcription polymerase chain reaction (RT-PCR), Western blot, and immunohistochemistry were used to investigate the expression of Trim32 in the damaged cortex...
July 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28916426/prognostic-relevance-of-tripartite-motif-containing-24-expression-in-colorectal-cancer
#13
Feng-Qin Wang, Yan Han, WenJuan Yao, Jing Yu
Colorectal cancer is one of the most frequent malignancies in the world. Tripartite motif containing 24 (TRIM24) is a member of the TRIM protein family and a coregulator for multiple nuclear receptors. Altered expression of TRIM24 has been shown in a spectrum of human cancers. However, the clinical role of TRIM24 in colorectal cancer remains unknown. Here, gene expression data in colorectal cancer and normal tissues were downloaded from Gene Expression Omnibus (GEO). Western blotting analysis was conducted to compare TRIM24 expression between colorectal cancer and non-cancerous tissues...
October 2017: Pathology, Research and Practice
https://www.readbyqxmd.com/read/28803007/associations-between-biosecurity-practices-and-bovine-digital-dermatitis-in-danish-dairy-herds
#14
Victor H S Oliveira, Jan T Sørensen, Peter T Thomsen
The relationship between biosecurity and digital dermatitis (DD) was evaluated in 8,269 cows from a convenience sample of 39 freestall dairy herds. The hypothesis was that poor implementation of biosecurity was associated with higher within-herd prevalence of DD. All lactating cows were scored as negative or positive for DD at the hind legs during milking in the milking parlor. Information about biosecurity was obtained through questionnaires addressed to farmers, on-farm observations, and information from the Danish Cattle Database (www...
August 9, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28759846/the-association-of-folate-and-depression-a-meta-analysis
#15
REVIEW
Ansley Bender, Kelsey E Hagan, Neal Kingston
BACKGROUND: Previous research suggested that folate levels play an important role in the etiology and course of depression. However, the literature has been inconsistent with regard to differences in folate level between individuals with and without depression. The present meta-analysis synthesized the results of previous studies to examine whether individuals with depression had lower levels of folate than individuals without depression. METHODS: Meta-analytic procedures were conducted in accordance with PRISMA guidelines...
December 2017: Journal of Psychiatric Research
https://www.readbyqxmd.com/read/28732120/the-prevalence-of-molar-incisor-hypomineralization-evidence-from-70-studies
#16
Dongdong Zhao, Bao Dong, Dandan Yu, Qiongqiong Ren, Yehuan Sun
OBJECTIVE: A growing number of studies have investigated the prevalence of Molar Incisor Hypomineralization (MIH) around the world. The aim of this study was to systematically estimate the pooled prevalence of MIH. METHODS: A comprehensive literature research was completed in English and Chinese databases. Random effect models were used to calculate the pooled prevalence. To address the heterogeneity, meta-regression, and sensitivity analyzes were conducted. Publication bias was estimated by trim and fill method...
July 21, 2017: International Journal of Paediatric Dentistry
https://www.readbyqxmd.com/read/28692844/comparative-bonding-ability-to-dentin-of-a-universal-adhesive-system-and-monomer-conversion-as-functions-of-extended-light-curing-times-and-storage
#17
Paula Costa Pinheiro Sampaio, Paula de Castro Kruly, Clara Cabral Ribeiro, Leandro Augusto Hilgert, Patrícia Nóbrega Rodrigues Pereira, Polliana Mendes Candia Scaffa, Vinicius Di Hipólito, Paulo Henrique Perlatti D'Alpino, Fernanda Cristina Pimentel Garcia
OBJECTIVES: The purpose of this in vitro study was to evaluate the bonding ability and monomer conversion of a universal adhesive system applied to dentin as functions of different curing times and storage. The results were compared among a variety of commercial adhesives. MATERIALS AND METHODS: Flat superficial dentin surfaces were exposed on human molars and assigned into one of the following adhesives (n = 15): total-etch Adper Single Bond 2 (SB) and Optibond Solo Plus (OS), self-etch Optibond All in One (OA) and Clearfil SE Bond (CSE), and Scotchbond Universal Adhesive in self-etch mode (SU)...
June 27, 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28627315/bioabsorbable-fixation-screw-for-proximal-interphalangeal-arthrodesis-of-lesser-toe-deformities
#18
Jascha Armin Wendelstein, Peter Goger, Peter Bock, Reinhard Schuh, Priv Doz, Hans-Jörg Trnka
BACKGROUND: Although standard fixation of proximal interphalangeal (PIP) arthrodesis by K-wire is relatively inexpensive and well established, it does have some drawbacks, making newer fixation devices interesting. The aim of this study was to clinically and radiologically assess the operative correction of the lesser toe deformation in the form of a PIP arthrodesis using a bioabsorbable 2.7-mm fixation screw. METHODS: From January 2011 until October 2013, 34 patients underwent a PIP arthrodesis using the TRIM-IT (Arthrex) 2...
September 2017: Foot & Ankle International
https://www.readbyqxmd.com/read/28611503/exploiting-phosphate-starved-cells-of-scenedesmus-sp-for-the-treatment-of-raw-sewage
#19
Swati Yewalkar-Kulkarni, Gayatri Gera, Sanjay Nene, Kiran Pandare, Bhaskar Kulkarni, Sanjay Kamble
Phosphate depletion is one of the favorable ways to enhance the sewage water treatment with the algae, however, detailed information is essential with respect to internal phosphate concentration and physiology of the algae. The growth rate of the phosphate-starved Scenedesmus cells was reduced drastically after 48 h. Indicating cells entered in the stationary phase of the growth cycle. Fourier Transform Infrared analysis of phosphate-starved Scenedesmus cells showed the reduction in internal phosphate concentration and an increase in carbohydrate/phosphate and carbohydrate/lipid ratio...
June 2017: Indian Journal of Microbiology
https://www.readbyqxmd.com/read/28592235/gene-centromere-mapping-in-meiotic-gynogenetic-european-seabass
#20
Münevver Oral, Julie Colléter, Michaël Bekaert, John B Taggart, Christos Palaiokostas, Brendan J McAndrew, Marc Vandeputte, Béatrice Chatain, Heiner Kuhl, Richard Reinhardt, Stefano Peruzzi, David J Penman
BACKGROUND: Fully isogenic lines in fish can be developed using "mitotic" gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families...
June 7, 2017: BMC Genomics
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