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Jennifer L Hanslick, Karen Lau, Kevin K Noguchi, John W Olney, Charles F Zorumski, Steven Mennerick, Nuri B Farber
Dimethyl sulfoxide (DMSO) is a solvent that is routinely used as a cryopreservative in allogous bone marrow and organ transplantation. We exposed C57Bl/6 mice of varying postnatal ages (P0-P30) to DMSO in order to study whether DMSO could produce apoptotic degeneration in the developing CNS. DMSO produced widespread apoptosis in the developing mouse brain at all ages tested. Damage was greatest at P7. Significant elevations above the background rate of apoptosis occurred at the lowest dose tested, 0.3 ml/kg...
April 2009: Neurobiology of Disease
Janusz Płomiński, Krzysztof Kwiatkowski
UNLABELLED: Between 1991 and 2002, we performed 156 primary total hip replacements in rheumatoid arthritis patients with protrusion acetabulum. The deficient acetabulum was reconstructed with autologous and allogous morsellized bone grafts from the femoral head. MATERIAL AND METHODS: Group 1 were 26 patients with reconstruction acetabulum deficient with autologous morsellized bone grafts and group 2 were 10 patients in witch we use allogous grafts. All patients were reviewed at an average follow-up of 5 years...
July 2006: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
Huseyin Ilhan, Ozkan Alatas, Baran Tokar, Omer çOlak, Ozgül Paşaoĝlu, Naim Koku
PURPOSE: The aim of this study was to evaluate the effect of allopurinol, methylene blue, and a monoclonal antibody to the adhesion molecule ICAM-1 in intestinal ischemia and reperfusion injury. METHODS: The rats were divided into 5 groups. CG (n = 8) was untreated controls, SISG (n = 11) received sterile isotonic saline solution, ICAMG (n = 12) received a monoclonal antibody to rat ICAM-1, ALLOG (n = 12) received allopurinol, and MBG (n = 14) received methylene blue...
November 2003: Journal of Pediatric Surgery
Xi-min Guo, Chang-yong Wang
This paper reviewed recent advances in pancreatic islet transplantation research, including islet isolation, purification, culture, cryopreservation and immunoisolation. Latest progresses in induction of pancreatic stem cell and embryonic stem cell to differentiate into insulin-producing islets were also introduced. On the basis of the present situation and future development of islet transplantation-based therapies for diabetes, the author thought that allogeous islet transplantation is a main choice for type I diabetes today and pancreatic stem cell transplantation for tomorrow...
February 2003: Zhongguo Yi Xue Ke Xue Yuan Xue Bao. Acta Academiae Medicinae Sinicae
N Odum, B Hofmann, B K Jakobsen, E Langhoff, N Morling, P Platz, L P Ryder, A Svejgaard
We studied the influence of HLA class I and class II antigens on the suppression of the MLR induced by primed lymphocytes (PLs) alloactivated in vitro. The suppression of 14 different PLs of 83 MLRs was analyzed. The PLs were primed against (i) HLA-DP (SB) (ii) HLA-DR/DQ or (iii) both HLA-DP and DR/DQ. The suppression was analyzed with special reference to the sharing of HLA-antigens between (i) the stimulator in the MLR and (ii) the stimulator generating the PL. HLA-DP and HLA-DR/DQ antigens were equally capable of generating suppressor cells...
January 1986: Tissue Antigens
J C Laurence, T Hamaoka, M Kitagawa
The presence of cell-free Ehrlich ascites tumor fluid in an appropriate concentration was shown to permit a proliferative response, strictly dependent on the presence of Thy-1-positive lymphocytes, to proceed when introduced on the initiation of mixed lymphocyte-tumor reaction of C57BL/6 lymphocytes and Mitomycin-C-treated C3H/He myeloma cells, while it clearly inhibited the development of cytotoxic killer T lymphocytes toward the priming allogeic tumor cells. The fluid had no effect, however, when added to normally primed spleen cells at the onset of cytotoxicity test...
June 1976: Gan. Gann; the Japanese Journal of Cancer Research
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