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Milton Cordeiro, Ana Rita Otrelo-Cardoso, Dmitri I Svergun, Petr V Konarev, João Carlos Lima, Teresa Santos-Silva, Pedro Viana Baptista
Selective base pairing is the foundation of DNA recognition. Here, we elucidate the molecular and structural details of a FRET-based two-component molecular beacon relying on Steady-state Fluorescence Spectroscopy, Small-angle X-ray Scattering (SAXS), Microscale Thermophoresis (MST) and Differential Electrophoretic Mobility. This molecular beacon was designed to detect the most common fusion sequences causing chronic myeloid leukemia, e14a2 and e13a2. The emission spectra indicate that the self-assembly of the different components of the biosensor occurs sequentially, triggered by the fully complementary target...
March 21, 2018: ACS Chemical Biology
Fernando Mitano, Amélia Nunes Sicsú, Luciana de Oliveira Sousa, Rarianne Carvalho Peruhype, Jaqueline Garcia de Almeida Ballestero, Pedro Fredemir Palha
OBJECTIVE: To analyze the discourses of health professionals about the obstacles in the process of detection and reporting of tuberculosis cases in Mozambique. METHOD: Qualitative exploratory study with a theoretical-methodological approach of Discourse Analysis of French matrix. The study was conducted in Mozambique in 2014 at three levels: central, provincial and district. The study included 15 health professionals, 4 physicians, 6 technicians and 5 nursing professionals, who worked in the National Tuberculosis Control Program, with more than 1 year of experience...
2018: Revista Brasileira de Enfermagem
Sandra González-Casado, Olga Martín-Belloso, Pedro Elez-Martínez, Robert Soliva-Fortuny
The application of pulsed electric fields (PEFs) to whole tomatoes is proposed as a pre-processing treatment to obtain purees with high health-related properties. Tomato fruit was subjected to different electric field strengths (0.4, 1.2 and 2 kV cm-1) and number of pulses (5, 18 and 30 pulses). Tomatoes were stored at 4 °C for 24 h after PEF processing and then ground and mixed with 5% olive oil. The resulting tomato-based product was subjected to in vitro gastrointestinal digestion. PEF treatments significantly increased the amount and bioaccessible fraction of carotenoids in the derived product...
March 21, 2018: Food & Function
Diego de Souza Gonçalves, Marina da Silva Ferreira, Susie Coutinho Liedke, Kamilla Xavier Gomes, Gabriel Afonso de Oliveira, Pedro Ernesto Lopes Leão, Gabriele Vargas Cesar, Sergio H Seabra, Juliana Reis Cortines, Arturo Casadevall, Leonardo Nimrichter, Gilberto Barbosa Domont, Magno Rodrigues Junqueira, Jose Mauro Peralta, Allan J Guimaraes
Acanthamoeba castellanii (Ac) are ubiquitously distributed in nature, and by contaminating medical devices such as heart valves and contact lenses, they cause a broad range of clinical presentations to humans. Although several molecules have been described to play a role in Ac pathogenesis, including parasite host-tissue invasion and escaping of host-defense, little information is available on their mechanisms of secretion. Herein, we describe the molecular components secreted by Ac, under different protein availability conditions to simulate host niches...
March 21, 2018: Virulence
Marilia N N Lima, Cleber C Melo-Filho, Gustavo C Cassiano, Bruno J Neves, Vinicius M Alves, Rodolpho C Braga, Pedro V L Cravo, Eugene N Muratov, Juliana Calit, Daniel Y Bargieri, Fabio T M Costa, Carolina H Andrade
Malaria is a life-threatening infectious disease caused by parasites of the genus Plasmodium , affecting more than 200 million people worldwide every year and leading to about a half million deaths. Malaria parasites of humans have evolved resistance to all current antimalarial drugs, urging for the discovery of new effective compounds. Given that the inhibition of deoxyuridine triphosphatase of Plasmodium falciparum ( Pf dUTPase) induces wrong insertions in plasmodial DNA and consequently leading the parasite to death, this enzyme is considered an attractive antimalarial drug target...
2018: Frontiers in Pharmacology
R Henrik Nilsson, Andy F S Taylor, Rachel I Adams, Christiane Baschien, Johan Bengtsson-Palme, Patrik Cangren, Claudia Coleine, Heide-Marie Daniel, Sydney I Glassman, Yuuri Hirooka, Laszlo Irinyi, Reda Iršėnaitė, Pedro M Martin-Sanchez, Wieland Meyer, Seung-Yoon Oh, Jose Paulo Sampaio, Keith A Seifert, Frantisek Sklenář, Dirk Stubbe, Sung-Oui Suh, Richard Summerbell, Sten Svantesson, Martin Unterseher, Cobus M Visagie, Michael Weiss, Joyce Hc Woudenberg, Christian Wurzbacher, Silke Van den Wyngaert, Neriman Yilmaz, Andrey Yurkov, Urmas Kõljalg, Kessy Abarenkov
Recent DNA-based studies have shown that the built environment is surprisingly rich in fungi. These indoor fungi - whether transient visitors or more persistent residents - may hold clues to the rising levels of human allergies and other medical and building-related health problems observed globally. The taxonomic identity of these fungi is crucial in such pursuits. Molecular identification of the built mycobiome is no trivial undertaking, however, given the large number of unidentified, misidentified, and technically compromised fungal sequences in public sequence databases...
2018: MycoKeys
Nicolas Gourdin, Marion Bossennec, Céline Rodriguez, Selena Vigano, Christelle Machon, Camilla Jandus, David Bauché, Julien Faget, Isabelle Durand, Nicolas Chopin, Olivier Tredan, Julien C Marie, Bertrand Dubois, Jérôme Guitton, Pedro Romero, Christophe Caux, Christine Ménétrier-Caux
The production of CD73-derived Adenosine (Ado) by Tregs, has been proposed as a resistance mechanism to anti-PD1 therapy in murine tumor models. We reported that Human Tregs express the ecto-nucleotidase CD39, that generates AMP from ATP, but do not express the AMPase CD73. In contrast, CD73 defined a subset of effector CD4+ T cells (Teffs), enriched in polyfunctional Th1.17 cells characterized by expression of CXCR3, CCR6 and MDR1 and production of IL-17A/IFN-γ/IL-22/GM-CSF. CD39+ Tregs selectively targeted CD73+ Teffs through cooperative degradation of ATP into Ado inhibiting and restricting the ability of CD73+ Teffs to secrete IL-17A...
March 20, 2018: Cancer Research
João Perdigão, Carla Silva, Jaciara Diniz, Catarina Pereira, Diana Machado, Jorge Ramos, Hugo Silva, Fernanda Abilleira, Clarice Brum, Ana J Reis, Maíra Macedo, João L Scaini, Ana B Silva, Leonardo Esteves, Rita Macedo, Fernando Maltez, Sofia Clemente, Elizabeth Coelho, Sofia Viegas, Paulo Rabna, Amabélia Rodrigues, Nuno Taveira, Luísa Jordao, Afrânio Kritski, José Lapa E Silva, Igor Mokrousov, David Couvin, Nalin Rastogi, Isabel Couto, Arnab Pain, Ruth McNerney, Taane G Clark, Andrea von Groll, Elis R Dalla-Costa, Maria Lúcia Rossetti, Pedro E A da Silva, Miguel Viveiros, Isabel Portugal
Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. To address this, we have assembled and analysed the largest CPLP M. tuberculosis molecular and drug susceptibility dataset, comprised by a total of 1447 clinical isolates, including 423 multidrug-resistant isolates, from five CPLP countries...
March 17, 2018: Infection, Genetics and Evolution
Nebiyu Hiruy, Muluken Melese, Dereje Habte, Degu Jerene, Zewdu Gashu, Genetu Alem, Ilili Jemal, Belay Tessema, Beza Belayneh, Pedro G Suarez
OBJECTIVES: This study compared the yield of TB among contacts of multidrug-resistant tuberculosis (MDR-TB) index cases with that of drug-sensitive TB (DS-TB) index cases in a program setting. METHODS: A comparative cross-sectional study was conducted among contacts of sputum-smear-positive new index DS-TB cases and MDR-TB index cases. After contacts were screened, we used GeneXpert for the diagnosis of TB. RESULTS: The study included 111 MDR-TB and 119 DS-TB index cases...
March 17, 2018: International Journal of Infectious Diseases: IJID
Rocío Checa, Ana María López-Beceiro, Ana Montoya, Juan Pedro Barrera, Nieves Ortega, Rosa Gálvez, Valentina Marino, Julia González, Ángeles Sonia Olmeda, Luis Eusebio Fidalgo, Guadalupe Miró
Piroplasmosis is caused by several species of protozoa such as the Babesia microti-like piroplasm (Bml), an emerging blood protozoan also known as Theileria annae or Babesia vulpes. Infection by Bml was first reported in dogs in Spain where it is endemic today. Recently, a high prevalence of Bml has been increasingly detected in red foxes (Vulpes vulpes) in European countries. The objective of this study was to determine infection levels of this parasite in foxes from Galicia, NW Spain, and ticks species infestation in these carnivores, where they are so far unknown...
March 15, 2018: Veterinary Parasitology
Elke von Son-de Fernex, Ángel Alonso-Díaz Miguel, Braulio Valles-de la Mora, Pedro Mendoza-de Gives, Epigmenio Castillo-Gallegos, Alejandro Zamilpa, Manases González-Cortazar
The aims of this study were: 1) to assess the anthelmintic effect of Gliricidia sepium on the establishment of C. punctata third-stage larvae (L3 ) in calves, and 2) to isolate and to elucidate an anti-exsheathment phytochemical from the plant offered during the trial. Twelve ¾ Holstein × Zebu calves were divided in two experimental groups: control (T1) and treatment (T2) (n = 6). After adaptation, each calf was infected with an oral dose of 400 C. punctata L3 /Kg LW. Basal diet consisted of Digitaria decumbens hay (6...
March 15, 2018: Veterinary Parasitology
Pedro A A Monteleone, Pedro F M Peregrino, Edmund C Baracat, Paulo C Serafini
INTRODUCTION: Assisted reproductive technologies (ARTs) are associated with potential risks, mainly related to multiple pregnancies, which are around 20% to 25%. Iatrogenic multiple pregnancies due to ovarian stimulation with multiples embryos transferred can be avoided by the elective single-embryo transfer (eSET), a growing practice worldwide. Adequately applied eSET, which impact on the incidence of complications without compromising treatment success, is still a challenge. The aim of this study was to compare the cumulative success rates of elective transfer of 2 embryos when transferred one by one (eSET), versus the success rates of elective double-embryo transfer (DET) in a single procedure, in a good prognosis population...
January 1, 2018: Reproductive Sciences
Angela Blasimann, Simon Eberle, Manuel Markus Scuderi
BACKGROUND:  Soccer is seen as highly intensive sport with an increased injury rate. Male adults are the players with the highest injury incidence. Accordingly, the importance of core muscle strengthening to prevent injury has increased in the past few years. Up to date, core muscle strengthening plays an important role in different prevention programs, such as the "FIFA 11 +". The aim of this systematic review was to investigate the effect of core muscle strengthening on injury rate in male adult soccer players, including at least the known and easy exercises "plank" and "side plank", on injury rate in male adult soccer players...
March 2018: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
María Del Pino, Amado Andrés, Ana Ávila Bernabéu, Joaquín de Juan-Rivera, Elvira Fernández, Juan de Dios García Díaz, Domingo Hernández, José Luño, Isabel Martínez Fernández, José Paniagua, Manuel Posada de la Paz, José Carlos Rodríguez-Pérez, Rafael Santamaría, Roser Torra, Joan Torras Ambros, Pedro Vidau, Josep-Vicent Torregrosa
Fabry disease (FD) is a rare, X-linked disorder caused by mutations in the GLA gene encoding the enzyme α-galactosidase A. Complete or partial deficiency in this enzyme leads to intracellular accumulation of globotriaosylceramide (Gb3) and other glycosphingolipids in many cell types throughout the body, including the kidney. Progressive accumulation of Gb3 in podocytes, endothelial cells, epithelial cells, and tubular cells contribute to the renal symptoms of FD, which manifest as proteinuria and reduced glomerular filtration rate leading to renal insufficiency...
March 16, 2018: Kidney & Blood Pressure Research
Hirotaka Iijima, Kanako Shimoura, Tomoki Aoyama, Masaki Takahashi
BACKGROUND: Stair climbing was suggested to be the first affected task in individuals with knee osteoarthritis (OA). RESEARCH QUESTION: This review aimed to identify consistent kinematic, kinetic, and spatiotemporal alterations of stair climbing exhibited by individuals with knee OA. METHODS: A literature search published until September 2017 was conducted in PubMed, PEDro, CINAHL, and Cochrane CENTRAL. Reviewer extracted data in accordance with the Cochrane Handbook...
March 8, 2018: Gait & Posture
Pedro Teixeira Castro, Ana Paula Pinho Matos, Osvaldo Luiz Aranda, Edson Marchiori, Haimon Diniz Lopes Alves, Alessandra Silveira Machado, Ricardo Tadeu Lopes, Heron Werner, Edward Araujo Júnior
OBJECTIVE: The aim of this study was to provide a three-dimensional (3D) microscopic reconstruction of morphological modifications of the fallopian tube (FT) following surgical sterilization (including tuboperitoneal fistula) and ectopic pregnancy (EP) using confocal microtomography (micro-CT). METHODS: Six specimens of FT from elective and emergency surgeries were selected: three remnants of the FT (RFT) from surgical sterilization, including one presenting tuboperitoneal fistula, and three FTs with EP...
March 20, 2018: Journal of Maternal-fetal & Neonatal Medicine
Catarina S H Jesus, Pedro Fernandes Cruz, Luis G Arnaut, Rui M M Brito, Carlos Serpa
The understanding of fast folding dynamics of single α-helices comes mostly from studies on rationally designed peptides displaying sequences with high helical propensity. The folding/unfolding dynamics and energetics of α-helix conformations in naturally occurring peptides remains largely unexplored. Here we report the study of a protein fragment analogue of the C-peptide from bovine pancreatic ribonuclease-A, RN80, a 13-amino acid residue peptide that adopts a highly populated helical conformation in aqueous solution...
March 20, 2018: Journal of Physical Chemistry. B
Nazanin Abolhassani, Julien Castioni, Valérie Santschi, Gérard Waeber, Pedro Marques-Vidal
BACKGROUND: Polypharmacy (PP) and excessive polypharmacy (EPP) are increasingly common and associated with risk of drug-drug interactions (DDIs). We aimed to measure the trends and determinants of PP and DDIs among patients discharged from the Department of Internal Medicine of the Lausanne University Hospital. METHODS: The retrospective study included 17,742 adult patients discharged between 2009 and 2015. Polypharmacy and EPP were defined as the concomitant prescription of five or more and ten or more drugs, respectively...
March 19, 2018: Journal of Patient Safety
Anabela G Silva, Pedro Sá Couto, Alexandra Queirós, Maritza Neto, Nelson P Rocha
STUDY DESIGN: cross-sectional. OBJECTIVE: This study aims to describe how pain at multiple body sites is associated after controlling for other predictive factors such as age, gender, sleeping hours, time spent in physical activity and time spent in screening based activities in adolescents aged 13 to 19 years old. SUMMARY OF BACKGROUND DATA: The prevalence of multisite pain in adolescents is high but studies investigating the patterns of association between painful body sites are scarce...
March 19, 2018: Spine
Chun-De Liao, Jau-Yih Tsauo, Hung-Chou Chen, Tsan-Hon Liou
OBJECTIVE: Extracorporeal shock wave therapy (ESWT), including radial shock wave (RaSW) and focused shock wave (FoSW) types, is widely used for managing tendinopathies. The difference in efficacy between the 2 shock wave characteristics with different dosage levels remains controversial, and the purpose of this meta-analysis was to examine it for patients with lower extremity (LE) tendinopathy. DESIGN: A comprehensive search of online databases and search engines was performed...
March 19, 2018: American Journal of Physical Medicine & Rehabilitation
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