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https://www.readbyqxmd.com/read/29793088/decellularized-and-matured-esophageal-scaffold-for-circumferential-esophagus-replacement-proof-of-concept-in-a-pig-model
#1
Guillaume Luc, Guillaume Charles, Caroline Gronnier, Magali Cabau, Charlotte Kalisky, Mallory Meulle, Reine Bareille, Samantha Roques, Lionel Couraud, Johanna Rannou, Laurence Bordenave, Denis Collet, Marlène Durand
Surgical resection of the esophagus requires sacrificing a long portion of it. Its replacement by the demanding gastric pull-up or colonic interposition techniques may be avoided by using short biologic scaffolds composed of decellularized matrix (DM). The aim of this study was to prepare, characterize, and assess the in vivo remodeling of DM and its clinical impact in a preclinical model. A dynamic chemical and enzymatic decellularization protocol of porcine esophagus was set up and optimized. The resulting DM was mechanically and biologically characterized by DNA quantification, histology, and histomorphometry techniques...
May 17, 2018: Biomaterials
https://www.readbyqxmd.com/read/29792904/monoclonal-antibody-dl11c8-identifies-adam23-as-a-component-of-lipid-raft-microdomains
#2
Zaine L M Borgonovo, Caroline F Ribeiro, Michele D M Costa, Ingrid L M Souza, Gustavo R Rossi, Monica V Alcantara, Max Ingberman, Luciano G Braga, Adriana F Mercadante, Lia S Nakao, Silvio M Zanata
A disintegrin and metalloprotease protein 23 (ADAM23) is a transmembrane type I glycoprotein involved with the development and maintenance of the nervous system, including neurite outgrowth, neuronal adhesion and differentiation and regulation of synaptic transmission. In addition, ADAM23 seems to participate in immune response and tumor establishment through interaction with different members of integrin receptors. Here, we describe a novel monoclonal antibody (DL11C8) that specifically recognizes the cysteine-rich domain of both pre-protein (100kDa) and mature (70 kDa) forms of ADAM23 from different species, including human, rodents and avian orthologs...
May 21, 2018: Neuroscience
https://www.readbyqxmd.com/read/29792674/partial-intrinsic-disorder-governs-the-dengue-capsid-protein-conformational-ensemble
#3
Priscilla L S Boon, Wuan Geok Saw, Xin Xiang Lim, Palur Venkata Raghuvamsi, Roland G Huber, Jan K Marzinek, Daniel A Holdbrook, Ganesh S Anand, Gerhard Grüber, Peter J Bond
The 11 kDa, positively charged dengue capsid protein (C protein) exists stably as a homodimer and co-localizes with the viral genome within mature viral particles. Its core is composed of four alpha helices encompassing a small hydrophobic patch that may interact with lipids, but approximately 20% of the protein at the N-terminus is intrinsically disordered, making it challenging to elucidate its conformational landscape. Here, we combine small-angle X-ray scattering (SAXS), amide hydrogen-deuterium exchange mass spectrometry (HDXMS), and atomic-resolution molecular dynamics (MD) simulations to probe the dynamics of dengue C proteins...
May 24, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29792383/donor-variation-and-optimization-of-human-mesenchymal-stem-cells-chondrogenesis-in-hyaluronic-acid
#4
Minwook Kim, Isaac E Erickson, Alice H Huang, Sean T Garrity, Robert L Mauck, David R Steinberg
Mesenchymal stem cells (MSCs) are an attractive cell type for cartilage repair that can undergo chondrogenesis in a variety of three dimensional (3D) scaffolds. Hyaluronic acid (HA) hydrogels provide a biologically relevant interface for cell encapsulation. While our previous studies have shown that MSC-laden HA constructs can mature in vitro to match native mechanical properties using cells from animal sources, clinical application will depend on the successful translation of these findings to human cells...
May 24, 2018: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/29792309/metabolic-and-physiologic-imaging-biomarkers-of-the-tumor-microenvironment-predict-treatment-outcome-with-radiation-or-a-hypoxia-activated-prodrug-in-mice
#5
Shingo Matsumoto, Shun Kishimoto, Keita Saito, Yoichi Takakusagi, Jeeva P Munasinghe, Nallathamby Devasahayam, Charles Hart, Robert J Gillies, James B Mitchell, Murali C Krishna
Pancreatic ductal adenocarcinoma (PDAC) is characterized by hypoxic niches that lead to treatment resistance. Therefore, studies of tumor oxygenation and metabolic profiling should contribute to improved treatment strategies. Here we define two imaging biomarkers that predict differences in tumor response to therapy: 1) partial oxygen pressure (pO2), measured by EPR imaging; and 2) [1-13C] pyruvate metabolism rate, measured by hyperpolarized 13C MRI. Three human PDAC xenografts with varying treatment sensitivity (Hs766t, MiaPaCa-2, and Su...
May 23, 2018: Cancer Research
https://www.readbyqxmd.com/read/29792274/ex-vivo-expanded-natural-regulatory-t-cells-from-patients-with-end-stage-renal-disease-or-kidney-transplantation-are-useful-for-autologous-cell-therapy
#6
Sybille Landwehr-Kenzel, Anne Zobel, Henrike Hoffmann, Niels Landwehr, Michael Schmueck-Henneresse, Thomas Schachtner, Andy Roemhild, Petra Reinke
Novel concepts employing autologous, ex vivo expanded natural regulatory T cells (nTreg) for adoptive transfer has potential to prevent organ rejection after kidney transplantation. However, the impact of dialysis and maintenance immunosuppression on the nTreg phenotype and peripheral survival is not well understood, but essential when assessing patient eligibility. The current study investigates regulatory T-cells in dialysis and kidney transplanted patients and the feasibility of generating a clinically useful nTreg product from these patients...
June 2018: Kidney International
https://www.readbyqxmd.com/read/29791855/myocardial-angiopoietin-1-controls-atrial-chamber-morphogenesis-by-spatiotemporal-degradation-of-cardiac-jelly
#7
Kyun Hoo Kim, Yoshikazu Nakaoka, Hellmut G Augustin, Gou Young Koh
The four-chamber structure of the mammalian heart is established during embryonic development. While key regulators for ventricular development are well studied, regulatory mechanisms for atrial chamber morphogenesis remain poorly understood. Here, we found that angiopoietin-1 (Angpt1), a vascular maturation factor, is highly and specifically expressed in atrial myocardium during heart development. Loss of myocardial Angpt1 in mouse embryo led to severe impairment in atrial chamber morphogenesis. We revealed that Angpt1 deficiency results in excessive deposition of cardiac jelly, which disturbs regulation of myocardial growth, thereby impairing maturation of atrial chambers...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791853/p27-kip1-modulates-axonal-transport-by-regulating-%C3%AE-tubulin-acetyltransferase-1-stability
#8
Giovanni Morelli, Aviel Even, Ivan Gladwyn-Ng, Romain Le Bail, Michal Shilian, Juliette D Godin, Elise Peyre, Bassem A Hassan, Arnaud Besson, Jean-Michel Rigo, Miguel Weil, Bert Brône, Laurent Nguyen
The protein p27Kip1 plays roles that extend beyond cell-cycle regulation during cerebral cortex development, such as the regulation of neuronal migration and neurite branching via signaling pathways that converge on the actin and microtubule cytoskeletons. Microtubule-dependent transport is essential for the maturation of neurons and the establishment of neuronal connectivity though synapse formation and maintenance. Here, we show that p27Kip1 controls the transport of vesicles and organelles along the axon of mice cortical projection neurons in vitro...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791837/phosphorylation-state-of-zfp24-controls-oligodendrocyte-differentiation
#9
Benayahu Elbaz, Joshua D Aaker, Sara Isaac, Anna Kolarzyk, Pedro Brugarolas, Amir Eden, Brian Popko
Zinc finger protein ZFP24, formerly known as ZFP191, is essential for oligodendrocyte maturation and CNS myelination. Nevertheless, the mechanism by which ZFP24 controls these processes is unknown. We demonstrate that ZFP24 binds to a consensus DNA sequence in proximity to genes important for oligodendrocyte differentiation and CNS myelination, and we show that this binding enhances target gene expression. We also demonstrate that ZFP24 DNA binding is controlled by phosphorylation. Phosphorylated ZFP24, which does not bind DNA, is the predominant form in oligodendrocyte progenitor cells...
May 22, 2018: Cell Reports
https://www.readbyqxmd.com/read/29791809/phagocytosis-what-s-on-the-menu
#10
Charlene E Lancaster, Cheuk Y Ho, Victoria E B Hipolito, Roberto J Botelho, Mauricio R Terebiznik
Phagocytosis is an evolutionarily conserved process. In Protozoa, phagocytosis fulfills a feeding mechanism, while in Metazoa, phagocytosis diversified to play multiple organismal roles, including immune defence, tissue homeostasis and remodeling. Accordingly, phagocytes display a high level of plasticity in their capacity to recognize, engulf and process targets that differ in composition and morphology. Here, we review how phagocytosis adapts to its multiple roles and discuss in particular the effect of target morphology in phagocytic uptake and phagosome maturation...
May 23, 2018: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/29791567/immunohistological-study-of-the-effect-of-vascular-endothelial-growth-factor-on-the-angiogenesis-of-mature-root-canals-in-rat-molars
#11
Cleber Keiti Nabeshima, José Edgar Valdivia, Hector Caballero-Flores, Victor Elias Arana-Chavez, Manoel Eduardo de Lima Machado
Tissue bioengineering has been applied to Endodontics to seek a more biological treatment. The presence of blood vessels is crucial for cell nutrition during tissue formation. Objective This study analysed the application of vascular endothelial growth factor (VEGF) in the angiogenesis of mature root canals. Material and methods Upper first molars of twelve 13-week old Wistar male rats were used. The root pulp of the mesiobuccal canal was removed and the root canal instrumented with K-files up to size #25. Periapical bleeding was induced into the root canal by introducing a #15 K-file beyond the apex...
2018: Journal of Applied Oral Science: Revista FOB
https://www.readbyqxmd.com/read/29791237/the-future-of-chronic-lymphocytic-leukemia-potential-directions-from-ash-2017
#12
Stefano Molica
Novel targeted therapies - including ibrutinib, venetoclax and idelalisib - have revolutionized the treatment landscape of chronic lymphocytic leukemia (CLL). Therefore, studying combinations of novel agents (NAs) with distinct mechanism of action and non-overlapping toxicities is challenging. Area covered: The 2017 American Society Hematology (ASH) Annual Meeting, has represented a showcase for several trials combining NAs. These studies are currently evaluating the efficacy NA plus anti-CD20 monoclonal antibody, NA plus NA (with or without anti-CD20 monoclonal antibody) and NA plus chemo-immunotherapy...
May 23, 2018: Expert Review of Hematology
https://www.readbyqxmd.com/read/29791172/-current-treatment-options-in-maturity-onset-diabetes-of-the-young
#13
Ludmila Brunerová, Jana Urbanová, Jan Brož
The discovery of MODY (Maturity-Onset Diabetes of the Young) and the elucidation of its heritability enabled more precise clinical characteristics of different MODY subtypes and led to understanding that glucokinase MODY (GCK-MODY) is not associated with vascular complications in long term follow-up, whereas MODY of transcription factors (e.g. HNF1A-MODY) is in case of bad metabolic control connected with the acceleration of particularly microvascular complications. There is a strong evidence of the needlessness of any specific antidiabetic treatment in prognosticaly favourable GCK-MODY (except for the pregnancy)...
2018: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/29791171/-diagnosis-of-mody-brief-overview-for-clinical-practice
#14
Jana Urbanová, Ludmila Brunerová, Jan Brož
Maturity Onset Diabetes of the Young (MODY) comprises inherited forms of diabetes mellitus caused by the mutations in the genes involved in the development, differentiation and function of beta-cells. The majority of patients with MODY remains misdiagnosed and erroneously classified as type 1 or type 2 diabetic patients. Correct MODY diagnosis is, however, essential since it enables individualization of treatment, assessment of the prognosis and identification of diabetes among patient´s relatives. Clinical presentation of MODY is highly variable and it could resemble other types of diabetes, thus identification of MODY patients might be difficult...
2018: Vnitr̆ní Lékar̆ství
https://www.readbyqxmd.com/read/29791044/what-happens-to-offspring-when-parents-are-inbred-old-or-had-a-poor-start-in-life-interactions-between-multiple-causes-of-parental-effects
#15
Regina Vega-Trejo, Loeske E B Kruuk, Michael D Jennions, Megan L Head
Parental effects on offspring performance have been attributed to many factors such as parental age, size, and condition. However, we know little about how these different parental characteristics interact to determine parental effects, or the extent to which their effect on offspring depends on either the sex of the parent or that of the offspring. Here we experimentally tested for effects of variation in parents' early diet and inbreeding levels, as well as effects of parental age, and for potential interactive effects of these three factors on key aspects of offspring development in the mosquitofish (Gambusia holbrooki)...
May 23, 2018: Journal of Evolutionary Biology
https://www.readbyqxmd.com/read/29791013/frontline-science-buprenorphine-decreases-ccl2-mediated-migration-of-cd14-cd16-monocytes
#16
Matias Jaureguiberry-Bravo, Lillie Lopez, Joan W Berman
HIV infection of the CNS causes neuroinflammation and damage that contributes to the development of HIV-associated neurocognitive disorders (HAND) in greater than 50% of HIV-infected individuals, despite antiretroviral therapy (ART). Opioid abuse is a major risk factor for HIV infection. It has been shown that opioids can contribute to increased HIV CNS pathogenesis, in part, by modulating the function of immune cells. HIV enters the CNS within two weeks after peripheral infection by transmigration of infected monocytes across the blood brain barrier (BBB)...
May 23, 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29790938/the-low-threshold-calcium-channel-cav3-2-mediates-burst-firing-of-mature-dentate-granule-cells
#17
Mael Dumenieu, Oleg Senkov, Andrey Mironov, Emmanuel Bourinet, Michael R Kreutz, Alexander Dityatev, Martin Heine, Arthur Bikbaev, Jeffrey Lopez-Rojas
Mature granule cells are poorly excitable neurons that were recently shown to fire action potentials, preferentially in bursts. It is believed that the particularly pronounced short-term facilitation of mossy fiber synapses makes granule cell bursting a very effective means of properly transferring information to CA3. However, the mechanism underlying the unique bursting behavior of mature granule cells is currently unknown. Here, we show that Cav3.2 T-type channels at the axon initial segment are responsible for burst firing of mature granule cells in rats and mice...
April 18, 2018: Cerebral Cortex
https://www.readbyqxmd.com/read/29790935/intake-and-feed-utilization-in-two-breeds-of-pregnant-beef-cows-fed-forages-with-high-fiber-concentrations
#18
M Jardstedt, A Hessle, P Nørgaard, L Frendberg, E Nadeau
Hereford and Charolais beef cows (n = 24 per breed) were used to study the effect of breed and to evaluate how late cut reed canarygrass (RC) and whole-crop oats plus urea (WCO) compared to late cut timothy (TG) with respect to feed intake and digestibility, rumination time, fecal particle size (PS) distribution, N excretion, and ruminal microbial CP production (MCP). The TG and RC were cut at flowering and WCO at hard dough stage of maturity. Cows were group-housed, six groups per breed, and fed the three diets ad libitum in three periods...
May 22, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29790929/mir172-downregulates-the-translation-of-cleistogamy-1-in-barley
#19
Nadia Anwar, Masaru Ohta, Takayuki Yazawa, Yutaka Sato, Chao Li, Akemi Tagiri, Mari Sakuma, Thomas Nussbaumer, Phil Bregitzer, Mohammad Pourkheirandish, Jianzhong Wu, Takao Komatsuda
Background and Aims: Floret opening in barley is induced by the swelling of the lodicule, a trait under the control of the cleistogamy1 (cly1) gene. The product of cly1 is a member of the APETALA2 (AP2) transcription factor family, which inhibits lodicule development. A sequence polymorphism at the miR172 target site within cly1 has been associated with variation in lodicule development and hence with the cleistogamous phenotype. It was unclear whether miR172 actually functions in cly1 regulation and, if it does, which miR172 gene contributes to cleistogamy...
May 22, 2018: Annals of Botany
https://www.readbyqxmd.com/read/29790869/the-effect-of-caffeine-on-the-ventilatory-response-to-hypercarbia-in-preterm-infants
#20
Thomas Rossor, Ravindra Bhat, Kamal Ali, Janet Peacock, Gerrard F Rafferty, Anne Greenough
BackgroundWe tested the hypotheses that caffeine therapy would increase the ventilatory response to hypercarbia in infants above the effect of maturation and those with a weaker ventilatory response to hypercarbia would be more likely to subsequently develop apnea that required treatment.MethodsInfants born at less than 34 weeks of gestation underwent a steady-state hypercarbic challenge using 0, 2, and 4% carbon dioxide soon after birth that was repeated at weekly intervals. The results of the initial study were compared between infants who did or did not subsequently develop apnea requiring treatment with caffeine...
May 23, 2018: Pediatric Research
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