keyword
MENU ▼
Read by QxMD icon Read
search

CNO

keyword
https://www.readbyqxmd.com/read/27911758/multimodal-imaging-for-dreadd-expressing-neurons-in-living-brain-and-their-application-to-implantation-of-ipsc-derived-neural-progenitors
#1
Bin Ji, Hiroyuki Kaneko, Takafumi Minamimoto, Haruhisa Inoue, Hiroki Takeuchi, Katsushi Kumata, Ming-Rong Zhang, Ichio Aoki, Chie Seki, Maiko Ono, Masaki Tokunaga, Satoshi Tsukamoto, Koji Tanabe, Ryong-Moon Shin, Takeharu Minamihisamatsu, Seiji Kito, Barry J Richmond, Tetsuya Suhara, Makoto Higuchi
: Chemogenetic manipulation of neuronal activities has been enabled by a designer receptor (designer receptor exclusively activated by designer drugs, DREADD) that is activated exclusively by clozapine-N-oxide (CNO). Here, we applied CNO as a functional reporter probe to positron emission tomography (PET) of DREADD in living brains. Mutant human M4 DREADD (hM4Di) expressed in transgenic (Tg) mouse neurons was visualized by PET with microdose [(11)C]CNO. Deactivation of DREADD-expressing neurons in these mice by nonradioactive CNO at a pharmacological dose could also be captured by arterial spin labeling MRI (ASL-MRI)...
November 9, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27893096/chemogenetic-activation-of-iprgcs-drives-changes-in-dark-adapted-scotopic-electroretinogram
#2
Nina Milosavljevic, Annette E Allen, Jasmina Cehajic-Kapetanovic, Robert J Lucas
Purpose: The purpose of this study was to investigate the impact of activating melanopsin-expressing intrinsically photosensitive retinal ganglion cells (ipRGCs) on dark-adapted (scotopic) electroretinograms (ERG). Methods: We used mice (Opn4Cre/+) expressing cre recombinase in melanopsin-expressing cells for a targeted gene delivery of a chemogenetic Gq-coupled receptor, hM3Dq, to ipRGCs. Intraperitoneal injection of clozapine N-oxide (CNO) at 5 mg/kg was used for acute activation of hM3Dq and thus excitation of ipRGCs in darkness...
November 1, 2016: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/27866960/ethanol-seeking-behavior-is-expressed-directly-through-an-extended-amygdala-to-midbrain-neural-circuit
#3
Melanie M Pina, Christopher L Cunningham
Abstinent alcohol-dependent individuals experience an enduring sensitivity to cue-induced craving and relapse to drinking. There is considerable evidence indicating that structures within the midbrain and extended amygdala are involved in this process. Individually, the ventral tegmental area (VTA) and the bed nucleus of the stria terminalis (BNST) have been shown to modulate cue-induced ethanol-seeking behavior. It is hypothesized that cue-induced seeking is communicated through a direct projection from the BNST to VTA...
November 17, 2016: Neurobiology of Learning and Memory
https://www.readbyqxmd.com/read/27835017/experimental-investigation-of-the-19-ne-p-%C3%AE-20-na-reaction-rate-and-implications-for-breakout-from-the-hot-cno-cycle
#4
J Belarge, S A Kuvin, L T Baby, J Baker, I Wiedenhöver, P Höflich, A Volya, J C Blackmon, C M Deibel, H E Gardiner, J Lai, L E Linhardt, K T Macon, E Need, B C Rasco, N Quails, K Colbert, D L Gay, N Keeley
The ^{19}Ne(p,γ)^{20}Na reaction is the second step of a reaction chain which breaks out from the hot CNO cycle, following the ^{15}O(α,γ)^{19}Ne reaction at the onset of x-ray burst events. We investigate the spectrum of the lowest proton-unbound states in ^{20}Na in an effort to resolve contradictions in spin-parity assignments and extract reliable information about the thermal reaction rate. The proton-transfer reaction ^{19}Ne(d,n)^{20}Na is measured with a beam of the radioactive isotope ^{19}Ne at an energy around the Coulomb barrier and in inverse kinematics...
October 28, 2016: Physical Review Letters
https://www.readbyqxmd.com/read/27822508/clozapine-n-oxide-administration-produces-behavioral-effects-in-long-evans-rats-implications-for-designing-dreadd-experiments
#5
Duncan A A MacLaren, Richard W Browne, Jessica K Shaw, Sandhya Krishnan Radhakrishnan, Prachi Khare, Rodrigo A España, Stewart D Clark
Clozapine N-oxide (CNO) is a ligand for a powerful chemogenetic system that can selectively inhibit or activate neurons; the so-called Designer Receptors Exclusively Activated by Designer Drugs (DREADD) system. This system consists of synthetic G-protein-coupled receptors, which are not believed to be activated by any endogenous ligand, but are activated by the otherwise inert CNO. However, it has previously been shown that the administration of CNO in humans and rats leads to detectable levels of the bioactive compounds clozapine and N-desmethylclozapine (N-Des)...
September 2016: ENeuro
https://www.readbyqxmd.com/read/27798132/gi-dreadd-expression-in-peripheral-nerves-produces-ligand-dependent-analgesia-as-well-as-ligand-independent-functional-changes-in-sensory-neurons
#6
Jami L Saloman, Nicole N Scheff, Lindsey M Snyder, Sarah E Ross, Brian M Davis, Michael S Gold
: Designer receptors exclusively activated by designer drugs (DREADDs) are an advanced experimental tool that could potentially provide a novel approach to pain management. In particular, expression of an inhibitory (Gi-coupled) DREADD in nociceptors might enable ligand-dependent analgesia. To test this possibility, TRPV1-cre mice were used to restrict expression of Gi-DREADDs to predominantly C-fibers. Whereas baseline heat thresholds in both male and female mice expressing Gi-DREADD were normal, 1 mg/kg clozapine-N-oxide (CNO) produced a significant 3 h increase in heat threshold that returned to baseline by 5 h after injection...
October 19, 2016: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/27759104/single-and-transient-ca-2-peaks-in-podocytes-do-not-induce-changes-in-glomerular-filtration-and-perfusion
#7
Sybille Koehler, Sebastian Brähler, Alexander Kuczkowski, Julia Binz, Matthias J Hackl, Henning Hagmann, Martin Höhne, Merly C Vogt, Claudia M Wunderlich, F Thomas Wunderlich, Frank Schweda, Bernhard Schermer, Thomas Benzing, Paul T Brinkkoetter
Chronic alterations in calcium (Ca(2+)) signalling in podocytes have been shown to cause proteinuria and progressive glomerular diseases. However, it is unclear whether short Ca(2+) peaks influence glomerular biology and cause podocyte injury. Here we generated a DREADD (Designer Receptor Exclusively Activated by a Designer Drug) knock-in mouse line to manipulate intracellular Ca(2+) levels. By mating to a podocyte-specific Cre driver we are able to investigate the impact of Ca(2+) peaks on podocyte biology in living animals...
October 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27745045/consider-pros-and-cons-in-bursary-change-says-cno
#8
Kat Keogh
Removing the student bursary from next year is sending nursing into 'uncharted territory', England's chief nursing officer (CNO) Jane Cummings said.
September 14, 2016: Nursing Standard
https://www.readbyqxmd.com/read/27730289/unexpectedly-high-incidences-of-chronic-non-bacterial-as-compared-to-bacterial-osteomyelitis-in-children
#9
A Schnabel, U Range, G Hahn, T Siepmann, R Berner, C M Hedrich
Historically, osteomyelitis was considered an infectious disorder. More recently, inflammatory mechanisms were recognized causing a significant proportion of pediatric osteomyelitis. This study was to compare characteristics of children with chronic non-bacterial (CNO) and bacterial osteomyelitis (BOM). A chart review of osteomyelitis patients from the departments of pediatrics, pediatric surgery, orthopedic surgery, and oral and maxillofacial surgery was conducted in a tertiary referral center, covering the years 2004-2014...
October 11, 2016: Rheumatology International
https://www.readbyqxmd.com/read/27708951/characteristics-and-outcomes-of-chronic-non-bacterial-osteitis-in-children
#10
Stefanie Wintrich, Gerd Horneff
OBJECTIVE: To determine patient characteristics, clinical presentation, pattern of involvement, treatment, and outcome of patients with chronic non-bacterial osteitis (CNO). MATERIAL AND METHODS: Consecutive cases of CNO were analyzed at a single center for pediatrics and adolescent medicine from 2006 to 2013 in terms of patient characteristics, clinical presentation, pattern of involvement, treatment, and outcome. RESULTS: We identified 32 children aged 1...
December 2015: European Journal of Rheumatology
https://www.readbyqxmd.com/read/27585682/chronic-nonbacterial-osteomyelitis-pathophysiological-concepts-and-current-treatment-strategies
#11
REVIEW
Sigrun R Hofmann, Anja Schnabel, Angela Rösen-Wolff, Henner Morbach, Hermann J Girschick, Christian M Hedrich
Chronic nonbacterial osteomyelitis (CNO) is an autoinflammatory bone disorder, covering a clinical spectrum with asymptomatic inflammation of single bones at the one end, and chronic recurrent multifocal osteomyelitis (CRMO) at the other end. The exact molecular pathophysiology of CNO remains largely unknown. Provided familial clusters and the association with inflammatory disorders of the skin and intestine suggest a genetic predisposition. Recently, profound dysregulation of cytokine responses was demonstrated in CRMO...
November 2016: Journal of Rheumatology
https://www.readbyqxmd.com/read/27584898/on-boarding-a-new-chief-nursing-officer-to-lead-a-magnet-redesignation-visit-the-value-of-relationships
#12
Kathy Cantu, Joyce A Batcheller
The chief nursing officer (CNO) is a critical senior executive in a hospital and serves as the architect of patient care. Recruiting, hiring, and on-boarding a new CNO present a challenge for any facility. Stakes are higher when the facility is replacing a CNO in the midst of its Magnet redesignation. How does a facility ensure success under these circumstances? This case study demonstrates how one organization was successful in meeting this challenge.
October 2016: Nursing Administration Quarterly
https://www.readbyqxmd.com/read/27450944/predictors-of-nodal-upstaging-in-clinical-node-negative-patients-with-penile-carcinoma-a-national-cancer-database-analysis
#13
Brian R Winters, Matthew N Mossanen, Sarah K Holt, Daniel W Lin, Jonathan L Wright
OBJECTIVE: To examine the risk factors associated with upstaging at inguinal lymph node dissection (ILND) in men with penile cancer and clinically negative lymph nodes (cN0) using a large US cancer database. METHODS: The National Cancer Data Base was queried from 1998 to 2012 to identify men with penile cancer who underwent ILND and had complete clinical or pathologic node status available. Lymphovascular invasion (LVI) was available after 2010. Multivariate logistic regression evaluated factors (cT stage, grade, LVI) associated with pathologic nodal upstaging in those with cN0 disease...
October 2016: Urology
https://www.readbyqxmd.com/read/27434999/deciphering-the-transcriptomic-response-of-fusarium-verticillioides-in-relation-to-nitrogen-availability-and-the-development-of-sugarcane-pokkah-boeng-disease
#14
Zhenyue Lin, Jihua Wang, Yixue Bao, Qiang Guo, Charles A Powell, Shiqiang Xu, Baoshan Chen, Muqing Zhang
Pokkah boeng, caused by Fusarium verticillioides, is a serious disease in sugarcane industry. The disease severity is related to the sugarcane genotype as well as environmental considerations, such as nitrogen application. The impact of the nitrogen source (ammonium sulfate, urea, or sodium nitrate) on sugarcane pokkah boeng disease and its pathogen was investigated in planta and fungal growth and sporulation production was measured in vitro. The results showed that ammonium and nitrate were beneficial to fungal mycelium growth, cell densities, and sporulation, which enhanced the disease symptoms of sugarcane pokkah boeng compared to urea fertilization...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27429450/a-collective-neurodynamic-optimization-approach-to-nonnegative-matrix-factorization
#15
Jianchao Fan, Jun Wang
Nonnegative matrix factorization (NMF) is an advanced method for nonnegative feature extraction, with widespread applications. However, the NMF solution often entails to solve a global optimization problem with a nonconvex objective function and nonnegativity constraints. This paper presents a collective neurodynamic optimization (CNO) approach to this challenging problem. The proposed collective neurodynamic system consists of a population of recurrent neural networks (RNNs) at the lower level and a particle swarm optimization (PSO) algorithm with wavelet mutation at the upper level...
July 18, 2016: IEEE Transactions on Neural Networks and Learning Systems
https://www.readbyqxmd.com/read/27424119/an-integrated-biological-approach-for-treatment-of-cyanidation-wastewater
#16
REVIEW
Lukhanyo Mekuto, S K O Ntwampe, Ata Akcil
The cyanidation process has been, and still remains, a profitable and highly efficient process for the recovery of precious metals from ores. However, this process has contributed to environmental deterioration and potable water reserve contamination due to the discharge of poorly treated, or untreated, cyanide containing wastewater. The process produces numerous cyanide complexes in addition to the gold cyanocomplex. Additionally, the discharge constituents also include hydrogen cyanide (HCN) - metallic complexes with iron, nickel, copper, zinc, cobalt and other metals; thiocyanate (SCN); and cyanate (CNO)...
November 15, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27416078/dreadds-suppress-seizure-like-activity-in-a-mouse-model-of-pharmacoresistant-epileptic-brain-tissue
#17
N Avaliani, M Andersson, A H Runegaard, D Woldbye, M Kokaia
Epilepsy is a neurological disorder with a prevalence of ≈1% of general population. Available antiepileptic drugs (AEDs) have multiple side effects and are ineffective in 30% of patients. Therefore, development of effective treatment strategies is highly needed, requiring drug-screening models that are relevant and reliable. We investigated novel chemogenetic approach, using DREADDs (designer receptors exclusively activated by designer drugs) as possible inhibitor of epileptiform activity in organotypic hippocampal slice cultures (OHSCs)...
August 4, 2016: Gene Therapy
https://www.readbyqxmd.com/read/27404844/chemogenetic-silencing-of-the-midline-and-intralaminar-thalamus-blocks-amygdala-kindled-seizures
#18
Evan Wicker, Patrick A Forcelli
Temporal lobe epilepsy is the most common form of medically-intractable epilepsy. While seizures in TLE originate in structures such as hippocampus, amygdala, and temporal cortex, they propagate through a crucial relay: the midline/intralaminar thalamus. Prior studies have shown that pharmacological inhibition of midline thalamus attenuates limbic seizures. Here, we examined a recently developed technology, Designer Receptors Exclusively Activated by Designer Drugs (DREADDs), as a means of chemogenetic silencing to attenuate limbic seizures...
September 2016: Experimental Neurology
https://www.readbyqxmd.com/read/27399458/179%C3%A2-chemogenetic-stimulation-of-the-lumbar-locomotor-network-enhances-motor-function-following-experimental-cervical-spinal-cord-injury-translational-relevance-for-a-novel-therapeutic-strategy
#19
Spyridon K Karadimas, Kajana Satkunendrarajah, Michael G Fehlings
INTRODUCTION: Cervical spinal cord injury (cSCI), the most common type of SCI, results in substantial motor impairment. No effective treatment options currently exist to restore motor function. The neural network responsible for locomotion resides within the lumbar region of the spinal cord. Interestingly, we discovered for the first time significant neural degeneration of the lumbar locomotor network during the chronic phase of cSCI. Here, we hypothesized that early chemogenetic stimulation of the lumbar glutamatergic cells may prevent degeneration of the locomotor central pattern generator and the associated motor decline after cSCI...
August 2016: Neurosurgery
https://www.readbyqxmd.com/read/27396082/nursing-leaders-welcome-cno-for-england-s-new-framework
#20
Nicola Merrifield, Steve Ford
No abstract text is available yet for this article.
May 25, 2016: Nursing Times
keyword
keyword
72952
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"