Nicole V Johnson, Steven C Wall, Kevin J Kramer, Clinton M Holt, Sivakumar Periasamy, Simone Richardson, Naveenchandra Suryadevara, Emanuele Andreano, Ida Paciello, Giulio Pierleoni, Giulia Piccini, Ying Huang, Pan Ge, James D Allen, Naoko Uno, Andrea R Shiakolas, Kelsey A Pilewski, Rachel S Nargi, Rachel E Sutton, Alexandria A Abu-Shmais, Robert Parks, Barton F Haynes, Robert H Carnahan, James E Crowe, Emanuele Montomoli, Rino Rappuoli, Alexander Bukreyev, Ted M Ross, Giuseppe A Sautto, Jason S McLellan, Ivelin S Georgiev
Three coronaviruses have spilled over from animal reservoirs into the human population and caused deadly epidemics or pandemics. The continued emergence of coronaviruses highlights the need for pan-coronavirus interventions for effective pandemic preparedness. Here, using LIBRA-seq, we report a panel of 50 coronavirus antibodies isolated from human B cells. Of these antibodies, 54043-5 was shown to bind the S2 subunit of spike proteins from alpha-, beta-, and deltacoronaviruses. A cryo-EM structure of 54043-5 bound to the pre-fusion S2 subunit of the SARS-CoV-2 spike defined an epitope at the apex of S2 that is highly conserved among betacoronaviruses...
January 16, 2024: bioRxiv