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https://www.readbyqxmd.com/read/27566709/streptomyces-atlanticus-sp-nov-a-novel-actinomycete-isolated-from-marine-sponge-aplysina-fulva-pallas-1766
#1
Fábio Sérgio Paulino Silva, Danilo Tosta Souza, Tiago Domingues Zucchi, Camila Cristiane Pansa, Rafael Leandro de Figueiredo Vasconcellos, Eduardo José Crevelin, Luiz Alberto Beraldo de Moraes, Itamar Soares Melo
The taxonomic position of a novel marine actinomycete isolated from a marine sponge, Aplysina fulva, which had been collected in the Archipelago of Saint Peter and Saint Paul (Equatorial Atlantic Ocean), was determined by using a polyphasic approach. The organism showed a combination of morphological and chemotaxonomic characteristics consistent with its classification in the genus Streptomyces and forms a distinct branch within the Streptomyces somaliensis 16S rRNA gene tree subclade. It is closely related to Streptomyces violascens ISP 5183(T) (97...
November 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/26358601/genome-sequence-of-streptomyces-caatingaensis-cmaa-1322-a-new-abiotic-stress-tolerant-actinomycete-isolated-from-dried-lake-bed-sediment-in-the-brazilian-caatinga-biome
#2
Suikinai Nobre Santos, Ranko Gacesa, Rodrigo Gouvêa Taketani, Paul F Long, Itamar Soares Melo
The genome sequence of the first Streptomyces species isolated from the Brazilian Caatinga is reported here. Genes related to environmental stress tolerance were prevalent and included many secondary metabolic gene clusters.
2015: Genome Announcements
https://www.readbyqxmd.com/read/26021916/draft-genome-sequence-of-bacillus-sp-strain-cmaa-1185-a-cellullolytic-bacterium-isolated-from-stain-house-lake-antarctic-peninsula
#3
Suikinai Nobre Santos, Vanessa Nessner Kavamura, Rodrigo Gouvêa Taketani, Rafael L F Vasconcellos, Tiago Domingues Zucchi, Itamar Soares Melo
The aim of this study was to report the genome sequence of the cellulolytic Bacillus sp. strain CMAA 1185, isolated from Stain House Lake, Antarctica.
2015: Genome Announcements
https://www.readbyqxmd.com/read/25744584/amycolatopsis-rhabdoformis-sp-nov-an-actinomycete-isolated-from-a-tropical-forest-soil
#4
Wallace Rafael Souza, Rafael Eduardo Silva, Michael Goodfellow, Kanungnid Busarakam, Fernanda Sales Figueiro, Douglas Ferreira, Edson Rodrigues-Filho, Luiz Alberto Beraldo Moraes, Tiago Domingues Zucchi
Strain SB026T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847T, Amycolatopsis plumensis NRRL B-24324T, Amycolatopsis tolypomycina DSM 44544T and Amycolatopsis vancoresmycina NRRL B-24208T. It was related most closely to A...
June 2015: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/25108263/absence-of-endo-1-4-%C3%AE-glucanase-kor1-alters-the-jasmonate-dependent-defence-response-to-pseudomonas-syringae-in-arabidopsis
#5
Jaime López-Cruz, Ivan Finiti, Emma Fernández-Crespo, Oscar Crespo-Salvador, Pilar García-Agustín, Carmen González-Bosch
During plant-pathogen interactions, the plant cell wall forms part of active defence against invaders. In recent years, cell wall-editing enzymes, associated with growth and development, have been related to plant susceptibility or resistance. Our previous work identified a role for several tomato and Arabidopsis endo-1,4-β-glucanases (EGs) in plant-pathogen interactions. Here we studied the response of the Arabidopsis thaliana T-DNA insertion mutant lacking EG Korrigan1 (KOR1) infected with Pseudomonas syringae...
October 15, 2014: Journal of Plant Physiology
https://www.readbyqxmd.com/read/24555625/inpatient-imaging-utilization-trends-of-the-past-decade
#6
Atul B Shinagare, Ivan K Ip, Sarah K Abbett, Richard Hanson, Steven E Seltzer, Ramin Khorasani
OBJECTIVE: We have previously reported inpatient imaging utilization trends at our institution from fiscal year (FY) 1984 through FY 2002. In this study, we assessed the trends in imaging utilization for inpatients from FY 2003 through FY 2012. MATERIALS AND METHODS: In this institutional review board-approved retrospective study performed at a 793-bed tertiary care academic institution, we reviewed imaging utilization in adult inpatients from October 1, 2002, through September 30, 2012 (FY 2003 through FY 2012), and recorded the gross number of imaging studies coded by modality (conventional [radiography and fluoroscopy], ultrasound, nuclear medicine, CT, and MRI) and associated relative value units (RVUs)...
March 2014: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/24442675/isolation-and-characterization-of-cellulolytic-bacteria-from-the-stain-house-lake-antarctica
#7
Itamar S Melo, Tiago D Zucchi, Rafael E Silva, Elke S D Vilela, Mirian Lobo Sáber, Luiz H Rosa, Vivian H Pellizari
The main aim was to evaluate the occurrence of cellulolytic bacteria from the Stain house Lake, located at Admiralty Bay, Antarctica. Thick cotton string served as a cellulose bait for the isolation of bacteria. A total of 52 bacterial isolates were recovered and tested for their cellulase activity, and two of them, isolates CMAA 1184 and CMAA 1185, showed significant cellulolytic activity on carboxymethylcellulose agar plates. Phylogenetic analysis placed the isolates into the Bacillus 16S ribosomal RNA gene subclade...
July 2014: Folia Microbiologica
https://www.readbyqxmd.com/read/24285649/draft-genome-sequence-of-pseudomonas-sp-strain-cmaa-1215-a-plant-growth-promoting-bacterium-isolated-from-a-brazilian-mangrove
#8
Rafael L F Vasconcellos, Rodrigo Mendes, Rodrigo G Taketani, Tiago D Zucchi, Itamar Soares Melo
The aim of this study was to sequence the genome of the plant growth-promoting Pseudomonas sp. strain CMAA 1215, an osmotolerant bacterium isolated from mangrove soil.
2013: Genome Announcements
https://www.readbyqxmd.com/read/20868213/the-azygos-anterior-cerebral-artery-bypass-double-reimplantation-technique-for-giant-anterior-communicating-artery-aneurysms
#9
Zaman Mirzadeh, Nader Sanai, Michael T Lawton
The authors introduce the azygos anterior cerebral artery (ACA) bypass as an option for revascularizing distal ACA territories, as part of a strategy to trap giant anterior communicating artery (ACoA) aneurysms. In this procedure, the aneurysm is exposed with an orbitozygomatic-pterional craniotomy and distal ACA vessels are exposed with a bifrontal craniotomy. The uninvolved contralateral A(2) segment of the ACA serves as a donor vessel for a short radial artery graft. The contralateral pericallosal artery (PcaA) and the callosomarginal artery (CmaA) are connected to the graft in the interhemispheric fissure using the double reimplantation technique...
April 2011: Journal of Neurosurgery
https://www.readbyqxmd.com/read/20855540/yibk-is-the-2-o-methyltransferase-trml-that-modifies-the-wobble-nucleotide-in-escherichia-coli-trna-leu-isoacceptors
#10
Alfonso Benítez-Páez, Magda Villarroya, Stephen Douthwaite, Toni Gabaldón, M-Eugenia Armengod
Transfer RNAs are the most densely modified nucleic acid molecules in living cells. In Escherichia coli, more than 30 nucleoside modifications have been characterized, ranging from methylations and pseudouridylations to more complex additions that require multiple enzymatic steps. Most of the modifying enzymes have been identified, although a few notable exceptions include the 2'-O-methyltransferase(s) that methylate the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)...
November 2010: RNA
https://www.readbyqxmd.com/read/18730215/your-office-assistant-and-the-cmaa
#11
W M Hebert
No abstract text is available yet for this article.
June 1969: California Medicine
https://www.readbyqxmd.com/read/18705854/extraction-of-high-quality-bacterial-rna-from-infected-leaf-tissue-for-bacterial-in-planta-gene-expression-analysis-by-multiplexed-fluorescent-northern-hybridization
#12
Alexander Schenk, Helge Weingart, Matthias S Ullrich
Plant pathogenic bacteria possess a large number of genes that allow them to grow and cause disease on plants. In planta gene expression analysis is important to understand the impact of these genes on bacterial virulence. A new mRNA-based approach using multiplexed Northern hybridization was developed. High-quality bacterial and plant total RNA was successfully isolated from leaf tissue infiltrated with Pseudomonas syringae. The procedure employs a new extraction buffer formulation containing glycine, sodium dodecylsulphate, cetyltrimethylammonium bromide, high-molecular-weight polyethylene glycol and beta-mercaptoethanol...
March 2008: Molecular Plant Pathology
https://www.readbyqxmd.com/read/17530782/cmae-a-transferase-shuttling-aminoacyl-groups-between-carrier-protein-domains-in-the-coronamic-acid-biosynthetic-pathway
#13
Eric R Strieter, Frédéric H Vaillancourt, Christopher T Walsh
During the biosynthesis of the cyclopropyl amino acid coronamic acid from l-allo-Ile by the phytotoxic Pseudomonas syringae, the aminoacyl group covalently attached to the pantetheinyl arm of CmaA is shuttled to the HS-pantetheinyl arm of the protein CmaD by the aminoacyltransferase CmaE. CmaE will only recognize deacylated CmaA for initial complexation. The aminoacyl group becomes covalently attached to the active site Cys of CmaE and can then be transferred out to the holo pantetheinylated form of CmaD. Both l-Val/l-[14C]Val exchange studies and MALDI-TOF support a reversible shuttling process...
June 26, 2007: Biochemistry
https://www.readbyqxmd.com/read/16357370/inpatient-radiology-utilization-trends-over-the-past-decade
#14
Amin Matin, David W Bates, Andrew Sussman, Pablo Ros, Richard Hanson, Ramin Khorasani
OBJECTIVE: The objective of our study was to assess patterns of use of radiology services for inpatients at our institution between 1993 and 2002. MATERIALS AND METHODS: We retrospectively reviewed the administrative data about adult inpatients for fiscal years 1993-2002 in a 721-bed tertiary care institution. Examinations were coded according to imaging technique: conventional (plain films and fluoroscopy), sonography, nuclear medicine, CT, or MRI. We assessed workload trends using relative value units (RVUs)...
January 2006: AJR. American Journal of Roentgenology
https://www.readbyqxmd.com/read/16154332/preparation-of-tc-99m-macroaggregated-albumin-from-recombinant-human-albumin-for-lung-perfusion-imaging
#15
A P Hunt, M Frier, R A Johnson, S Berezenko, A C Perkins
Human serum albumin (HSA) extracted from pooled blood taken from human donors is used in the production of (99m)Tc-labelled macroaggregated albumin (MAA) for lung perfusion imaging. However, concerns for the safety of blood-derived products due to potential contamination by infective agents (e.g. new variant CJD), make alternative production methods necessary. Recombinant DNA technology is a promising method of albumin production avoiding problems associated with human-derived HSA. This paper presents results comparing MAA prepared from recombinant human albumin (rHA, Recombumin) (rMAA) with in-house produced HSA MAA (hMAA) and commercially available MAA (cMAA)...
January 2006: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/16121186/cryptic-chlorination-by-a-non-haem-iron-enzyme-during-cyclopropyl-amino-acid-biosynthesis
#16
Frédéric H Vaillancourt, Ellen Yeh, David A Vosburg, Sarah E O'Connor, Christopher T Walsh
Enzymatic incorporation of chlorine, bromine or iodine atoms occurs during the biosynthesis of more than 4,000 natural products. Halogenation can have significant consequences for the bioactivity of these products so there is great interest in understanding the biological catalysts that perform these reactions. Enzymes that halogenate unactivated aliphatic groups have not previously been characterized. Here we report the activity of five proteins-CmaA, CmaB, CmaC, CmaD and CmaE-in the construction of coronamic acid (CMA; 1-amino-1-carboxy-2-ethylcyclopropane), a constituent of the phytotoxin coronatine synthesized by the phytopathogenic bacterium Pseudomonas syringae...
August 25, 2005: Nature
https://www.readbyqxmd.com/read/14964530/identification-and-characterization-of-a-well-defined-series-of-coronatine-biosynthetic-mutants-of-pseudomonas-syringae-pv-tomato-dc3000
#17
David M Brooks, Gustavo Hernández-Guzmán, Andrew P Kloek, Francisco Alarcón-Chaidez, Aswathy Sreedharan, Vidhya Rangaswamy, Alejandro Peñaloza-Vázquez, Carol L Bender, Barbara N Kunkel
To identify Pseudomonas syringae pv. tomato genes involved in pathogenesis, we carried out a screen for Tn5 mutants of P. syringae pv. tomato DC3000 with reduced virulence on Arabidopsis thaliana. Several mutants defining both known and novel virulence loci were identified. Six mutants contained insertions in biosynthetic genes for the phytotoxin coronatine (COR). The P. syringae pv. tomato DC3000 COR genes are chromosomally encoded and are arranged in two separate clusters, which encode enzymes responsible for the synthesis of coronafacic acid (CFA) or coronamic acid (CMA), the two defined intermediates in COR biosynthesis...
February 2004: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/14679222/characterization-of-cmaa-an-adenylation-thiolation-didomain-enzyme-involved-in-the-biosynthesis-of-coronatine
#18
Robin Couch, Sarah E O'Connor, Heather Seidle, Christopher T Walsh, Ronald Parry
Several pathovars of Pseudomonas syringae produce the phytotoxin coronatine (COR), which contains an unusual amino acid, the 1-amino-2-ethylcyclopropane carboxylic acid called coronamic acid (CMA), which is covalently linked to a polyketide-derived carboxylic acid, coronafacic acid, by an amide bond. The region of the COR biosynthetic gene cluster proposed to be responsible for CMA biosynthesis was resequenced, and errors in previously deposited cmaA sequences were corrected. These efforts allowed overproduction of P...
January 2004: Journal of Bacteriology
https://www.readbyqxmd.com/read/11881279/-role-of-sperm-antibodies-and-cellular-autoimmunity-to-sperm-in-the-pathogenesis-of-male-infertility
#19
J Madar, V Urbánek, A Chaloupková, K Nouza, R Kinský
OBJECTIVE: To test the hypothesis of relationship between sperm pathology and elevated humoral and/or cell-mediated antisperm autoimmunity in male partners from infertile couples. DESIGN: Analytic study. SETTING: Department of Immunobiology, Institute for the Care of Mother and Child, Prague. METHODS: Sperm samples were evaluated according to WHO rules. Sperm-bound antisperm autoantibodies (ASA) were determined by SpermMar Test (FertiPro N...
January 2002: Ceská Gynekologie
https://www.readbyqxmd.com/read/10844652/oxygenated-mycolic-acids-are-necessary-for-virulence-of-mycobacterium-tuberculosis-in-mice
#20
E Dubnau, J Chan, C Raynaud, V P Mohan, M A Lanéelle, K Yu, A Quémard, I Smith, M Daffé
Members of the Mycobacterium tuberculosis group synthesize a family of long-chain fatty acids, mycolic acids, which are located in the cell envelope. These include the non-oxygenated alpha-mycolic acid and the oxygenated keto- and methoxymycolic acids. The function in bacterial virulence, if any, of these various types of mycolic acids is unknown. We have constructed a mutant strain of M. tuberculosis with an inactivated hma (cmaA, mma4) gene; this mutant strain no longer synthesizes oxygenated mycolic acids, has profound alterations in its envelope permeability and is attenuated in mice...
May 2000: Molecular Microbiology
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