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Leonardo Jose Silva, Danilo Tosta Souza, Diego Bonaldo Genuario, Harold Alexander Vargas Hoyos, Suikinai Nobre Santos, Luiz Henrique Rosa, Tiago Domingues Zucchi, Itamar Soares Melo
A novel actinobacterium, designated strain CMAA 1533(T), was isolated from the rhizosphere of Deschampsia antarctica collected at King George Island, Antarctic Peninsula. Strain CMAA 1533(T) was found to grow over a wide range of temperatures (4-28 °C) and pH (4-10). Macroscopically, the colonies were observed to be circular shaped, smooth, brittle and opaque-cream on most of the culture media tested. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CMAA 1533(T) belongs to the family Nocardiaceae and forms a distinct phyletic line within the genus Rhodococcus...
November 15, 2017: Antonie Van Leeuwenhoek
Hitomi Taira, Satoru Taguchi
Photo-damage to photosystem II (PSII) from ultraviolet radiation (UVR) was determined using chlorophyll fluorescence in relation to sunscreen factors on the dinoflagellate Scrippsiella sweeneyae based on the cellular mycosporine-like amino acid contents (CMAAs, cell) and cell diameter (=light path, d). Three different CMAAs, cell were prepared by acclimating cells to three levels (30.8, 15.2, and 7.7Wm(-2)) of photosynthetically active radiation (PAR). PAR-acclimated cells were exposed to PAR (0.64Wm(-2))+UVR (3...
September 2017: Journal of Photochemistry and Photobiology. B, Biology
Rafael L F Vasconcellos, Suikinai Nobre Santos, Tiago Domingues Zucchi, Fábio Sérgio Paulino Silva, Danilo Tosta Souza, Itamar Soares Melo
Strain CMAA 1215T , a Gram-reaction-negative, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 × 1.3-1.9 µm) bacterium, was isolated from mangrove sediments, Cananéia Island, Brazil. Analysis of the 16S rRNA gene sequences showed that strain CMAA 1215T forms a distinct phyletic line within the Pseudomonas putida subclade, being closely related to P. plecoglossicida ATCC 700383T , P. monteilii NBRC 103158T , and P. taiwanensis BCRC 17751T of sequence similarity of 98.86, 98...
October 2017: Archives of Microbiology
Danilo Tosta Souza, Fábio Sérgio Paulino da Silva, Leonardo José da Silva, Eduardo José Crevelin, Luiz Alberto Beraldo Moraes, Tiago Domingues Zucchi, Itamar Soares Melo
A novel marine actinomycete, designated strain CMAA 1452T, was isolated from the sponge Scopalina ruetzleri collected from Saint Peter and Saint Paul Archipelago, in Brazil, and subjected to a polyphasic taxonomic investigation. The organism formed a distinct phyletic line in the Saccharopolyspora 16S rRNA gene tree and had chemotaxonomic and morphological properties consistent with its classification in this genus. It was found to be closely related to Saccharopolyspora dendranthemae KLBMP 1305T (99.5% 16S rRNA gene sequence similarity) and shared similarities of 99...
June 2017: International Journal of Systematic and Evolutionary Microbiology
Vanessa Nessner Kavamura, Suikinai Nobre Santos, Rodrigo Gouvêa Taketani, Rafael Leandro Figueiredo Vasconcellos, Itamar Soares Melo
The strain of Bacillus sp. CMAA 1363 was isolated from the Brazilian Caatinga biome and showed plant growth-promoting traits and ability to promote maize growth under drought stress. Sequencing revealed genes involved in stress response and plant growth promotion. These genomic features might aid in the protection of plants against the negative effects imposed by drought.
February 2, 2017: Genome Announcements
Fábio Sérgio Paulino Silva, Danilo Tosta Souza, Tiago Domingues Zucchi, Camila Cristiane Pansa, Rafael Leandro de Figueiredo Vasconcellos, Eduardo José Crevelin, Luiz Alberto Beraldo de Moraes, Itamar Soares Melo
The taxonomic position of a novel marine actinomycete isolated from a marine sponge, Aplysina fulva, which had been collected in the Archipelago of Saint Peter and Saint Paul (Equatorial Atlantic Ocean), was determined by using a polyphasic approach. The organism showed a combination of morphological and chemotaxonomic characteristics consistent with its classification in the genus Streptomyces and forms a distinct branch within the Streptomyces somaliensis 16S rRNA gene tree subclade. It is closely related to Streptomyces violascens ISP 5183(T) (97...
November 2016: Antonie Van Leeuwenhoek
Suikinai Nobre Santos, Ranko Gacesa, Rodrigo Gouvêa Taketani, Paul F Long, Itamar Soares Melo
The genome sequence of the first Streptomyces species isolated from the Brazilian Caatinga is reported here. Genes related to environmental stress tolerance were prevalent and included many secondary metabolic gene clusters.
2015: Genome Announcements
Suikinai Nobre Santos, Vanessa Nessner Kavamura, Rodrigo Gouvêa Taketani, Rafael L F Vasconcellos, Tiago Domingues Zucchi, Itamar Soares Melo
The aim of this study was to report the genome sequence of the cellulolytic Bacillus sp. strain CMAA 1185, isolated from Stain House Lake, Antarctica.
2015: Genome Announcements
Wallace Rafael Souza, Rafael Eduardo Silva, Michael Goodfellow, Kanungnid Busarakam, Fernanda Sales Figueiro, Douglas Ferreira, Edson Rodrigues-Filho, Luiz Alberto Beraldo Moraes, Tiago Domingues Zucchi
Strain SB026T was isolated from Brazilian rainforest soil and its taxonomic position established using data from a polyphasic study. The organism showed a combination of chemotaxonomic and morphological features consistent with its classification in the genus Amycolatopsis and formed a branch in the Amycolatopsis 16S rRNA gene tree together with Amycolatopsis bullii NRRL B-24847T, Amycolatopsis plumensis NRRL B-24324T, Amycolatopsis tolypomycina DSM 44544T and Amycolatopsis vancoresmycina NRRL B-24208T. It was related most closely to A...
June 2015: International Journal of Systematic and Evolutionary Microbiology
Jaime López-Cruz, Ivan Finiti, Emma Fernández-Crespo, Oscar Crespo-Salvador, Pilar García-Agustín, Carmen González-Bosch
During plant-pathogen interactions, the plant cell wall forms part of active defence against invaders. In recent years, cell wall-editing enzymes, associated with growth and development, have been related to plant susceptibility or resistance. Our previous work identified a role for several tomato and Arabidopsis endo-1,4-β-glucanases (EGs) in plant-pathogen interactions. Here we studied the response of the Arabidopsis thaliana T-DNA insertion mutant lacking EG Korrigan1 (KOR1) infected with Pseudomonas syringae...
October 15, 2014: Journal of Plant Physiology
Atul B Shinagare, Ivan K Ip, Sarah K Abbett, Richard Hanson, Steven E Seltzer, Ramin Khorasani
OBJECTIVE: We have previously reported inpatient imaging utilization trends at our institution from fiscal year (FY) 1984 through FY 2002. In this study, we assessed the trends in imaging utilization for inpatients from FY 2003 through FY 2012. MATERIALS AND METHODS: In this institutional review board-approved retrospective study performed at a 793-bed tertiary care academic institution, we reviewed imaging utilization in adult inpatients from October 1, 2002, through September 30, 2012 (FY 2003 through FY 2012), and recorded the gross number of imaging studies coded by modality (conventional [radiography and fluoroscopy], ultrasound, nuclear medicine, CT, and MRI) and associated relative value units (RVUs)...
March 2014: AJR. American Journal of Roentgenology
Itamar S Melo, Tiago D Zucchi, Rafael E Silva, Elke S D Vilela, Mirian Lobo Sáber, Luiz H Rosa, Vivian H Pellizari
The main aim was to evaluate the occurrence of cellulolytic bacteria from the Stain house Lake, located at Admiralty Bay, Antarctica. Thick cotton string served as a cellulose bait for the isolation of bacteria. A total of 52 bacterial isolates were recovered and tested for their cellulase activity, and two of them, isolates CMAA 1184 and CMAA 1185, showed significant cellulolytic activity on carboxymethylcellulose agar plates. Phylogenetic analysis placed the isolates into the Bacillus 16S ribosomal RNA gene subclade...
July 2014: Folia Microbiologica
Rafael L F Vasconcellos, Rodrigo Mendes, Rodrigo G Taketani, Tiago D Zucchi, Itamar Soares Melo
The aim of this study was to sequence the genome of the plant growth-promoting Pseudomonas sp. strain CMAA 1215, an osmotolerant bacterium isolated from mangrove soil.
2013: Genome Announcements
Zaman Mirzadeh, Nader Sanai, Michael T Lawton
The authors introduce the azygos anterior cerebral artery (ACA) bypass as an option for revascularizing distal ACA territories, as part of a strategy to trap giant anterior communicating artery (ACoA) aneurysms. In this procedure, the aneurysm is exposed with an orbitozygomatic-pterional craniotomy and distal ACA vessels are exposed with a bifrontal craniotomy. The uninvolved contralateral A(2) segment of the ACA serves as a donor vessel for a short radial artery graft. The contralateral pericallosal artery (PcaA) and the callosomarginal artery (CmaA) are connected to the graft in the interhemispheric fissure using the double reimplantation technique...
April 2011: Journal of Neurosurgery
Alfonso Benítez-Páez, Magda Villarroya, Stephen Douthwaite, Toni Gabaldón, M-Eugenia Armengod
Transfer RNAs are the most densely modified nucleic acid molecules in living cells. In Escherichia coli, more than 30 nucleoside modifications have been characterized, ranging from methylations and pseudouridylations to more complex additions that require multiple enzymatic steps. Most of the modifying enzymes have been identified, although a few notable exceptions include the 2'-O-methyltransferase(s) that methylate the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA)...
November 2010: RNA
W M Hebert
No abstract text is available yet for this article.
June 1969: California Medicine
Alexander Schenk, Helge Weingart, Matthias S Ullrich
Plant pathogenic bacteria possess a large number of genes that allow them to grow and cause disease on plants. In planta gene expression analysis is important to understand the impact of these genes on bacterial virulence. A new mRNA-based approach using multiplexed Northern hybridization was developed. High-quality bacterial and plant total RNA was successfully isolated from leaf tissue infiltrated with Pseudomonas syringae. The procedure employs a new extraction buffer formulation containing glycine, sodium dodecylsulphate, cetyltrimethylammonium bromide, high-molecular-weight polyethylene glycol and beta-mercaptoethanol...
March 2008: Molecular Plant Pathology
Eric R Strieter, Frédéric H Vaillancourt, Christopher T Walsh
During the biosynthesis of the cyclopropyl amino acid coronamic acid from l-allo-Ile by the phytotoxic Pseudomonas syringae, the aminoacyl group covalently attached to the pantetheinyl arm of CmaA is shuttled to the HS-pantetheinyl arm of the protein CmaD by the aminoacyltransferase CmaE. CmaE will only recognize deacylated CmaA for initial complexation. The aminoacyl group becomes covalently attached to the active site Cys of CmaE and can then be transferred out to the holo pantetheinylated form of CmaD. Both l-Val/l-[14C]Val exchange studies and MALDI-TOF support a reversible shuttling process...
June 26, 2007: Biochemistry
Amin Matin, David W Bates, Andrew Sussman, Pablo Ros, Richard Hanson, Ramin Khorasani
OBJECTIVE: The objective of our study was to assess patterns of use of radiology services for inpatients at our institution between 1993 and 2002. MATERIALS AND METHODS: We retrospectively reviewed the administrative data about adult inpatients for fiscal years 1993-2002 in a 721-bed tertiary care institution. Examinations were coded according to imaging technique: conventional (plain films and fluoroscopy), sonography, nuclear medicine, CT, or MRI. We assessed workload trends using relative value units (RVUs)...
January 2006: AJR. American Journal of Roentgenology
A P Hunt, M Frier, R A Johnson, S Berezenko, A C Perkins
Human serum albumin (HSA) extracted from pooled blood taken from human donors is used in the production of (99m)Tc-labelled macroaggregated albumin (MAA) for lung perfusion imaging. However, concerns for the safety of blood-derived products due to potential contamination by infective agents (e.g. new variant CJD), make alternative production methods necessary. Recombinant DNA technology is a promising method of albumin production avoiding problems associated with human-derived HSA. This paper presents results comparing MAA prepared from recombinant human albumin (rHA, Recombumin) (rMAA) with in-house produced HSA MAA (hMAA) and commercially available MAA (cMAA)...
January 2006: European Journal of Pharmaceutics and Biopharmaceutics
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