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https://www.readbyqxmd.com/read/28334050/improving-transcriptome-de-novo-assembly-by-using-a-reference-genome-of-a-related-species-translational-genomics-from-oil-palm-to-coconut
#1
Alix Armero, Luc Baudouin, Stéphanie Bocs, Dominique This
The palms are a family of tropical origin and one of the main constituents of the ecosystems of these regions around the world. The two main species of palm represent different challenges: coconut (Cocos nucifera L.) is a source of multiple goods and services in tropical communities, while oil palm (Elaeis guineensis Jacq) is the main protagonist of the oil market. In this study, we present a workflow that exploits the comparative genomics between a target species (coconut) and a reference species (oil palm) to improve the transcriptomic data, providing a proteome useful to answer functional or evolutionary questions...
2017: PloS One
https://www.readbyqxmd.com/read/28333069/environmental-enrichment-in-kennelled-pit-bull-terriers-canis-lupus-familiaris
#2
Jenna Kiddie, Anna Bodymore, Alex Dittrich
Although social enrichment can be considered beneficial in helping dogs cope with the kennel environment, when taking individual needs into account, it places a large demand on the carers and may not be appropriate in under-resourced kennels. Some kennels are also designed in such a way that there is too much social interaction, in that individuals cannot choose to distance themselves from conspecifics. This study therefore aimed to assess the effects of easily accessible enrichment on the behaviour of kennelled Pit Bull Terrier type dogs rescued from a dog-fighting ring in the Philippines...
March 23, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28330300/carbon-quantum-dots-shuttle-electrons-to-the-anode-of-a-microbial-fuel-cell
#3
A S Vishwanathan, Kartik S Aiyer, L A A Chunduri, K Venkataramaniah, S Siva Sankara Sai, Govind Rao
Electrodes based on graphite, graphene, and carbon nanomaterials have been used in the anode chamber of microbial fuel cells (MFCs). Carbon quantum dots (C-dots) are a class of versatile nanomaterials hitherto not reported in MFCs. C-dots previously synthesized from coconut husk were reported to possess hydroxyl and carboxyl functional groups on their surface. The presence of these functional groups on a carbon matrix conferred on the C-dots the ability to conduct and transfer electrons. Formation of silver nanoparticles from silver nitrate upon addition of C-dots confirmed their reducing ability...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330211/cellulase-production-using-natural-medium-and-its-application-on-enzymatic-hydrolysis-of-thermo-chemically-pretreated-biomass
#4
Shivani Sharma, Vinay Sharma, Arindam Kuila
Lignocellulosic bioethanol is an important renewable fuel for transportation purpose. Commercial production of lignocellulosic bioethanol mainly depends on cost of cellulase production, efficient pretreatment and enzymatic hydrolysis process. In the present study cellulase production from Aspergillus niger under submerged fermentation (SmF) was optimized using coconut water as natural medium. Maximum cellulase production (0.53 IU/mL) was achieved within 3 days of incubation using 8 % (w/v) waste paper and 0...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28330188/purification-and-characterization-of-a-surfactant-compatible-lipase-from-aspergillus-tamarii-jgif06-exhibiting-energy-efficient-removal-of-oil-stains-from-polycotton-fabric
#5
Arijit Das, Srividya Shivakumar, Sourav Bhattacharya, Sujina Shakya, S S Swathi
An extracellular lipase with 23,666.66 U/ml/min activity was produced by Aspergillus tamarii JGIF06 under submerged fermentation in mineral salt medium containing coconut oil (2.5 % v/v), tryptone (2 % w/v) and ammonium chloride (2 % w/v), with initial pH of 5 ± 0.2, incubated at 25 °C for 7 days on a rotary shaker at 120 rpm. A 7.9-fold increase in lipase-specific activity was recorded after purification by DEAE Sepharose ion exchange and Sephadex G200 column chromatography. The apparent molecular mass of this enzyme was revealed as 50 kDa by sodium dodecyl sulphate polyacrylamide gel electrophoresis...
December 2016: 3 Biotech
https://www.readbyqxmd.com/read/28328018/abundance-correction-for-mass-discrimination-effects-in-polymer-mass-spectra
#6
Martin S Engler, Sarah Crotty, Markus J Barthel, Christian Pietsch, Ulrich S Schubert, Sebastian Böcker
RATIONALE: Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is frequently used to analyze homo- and copolymers, i.e. for computing copolymer fingerprints. However, the oligomer abundances are influenced by mass discrimination, i.e. mass- and composition-dependent ionization. We have developed a computational method to correct the abundance bias caused by the mass discrimination. METHODS: MALDI-TOFMS in combination with computational methods was used to investigate three random copolymers with different ratios of styrene and isoprene...
May 30, 2016: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/28319904/sensitivity-enhancement-of-graphene-zinc-oxide-nanocomposite-based-electrochemical-impedance-genosensor-for-single-stranded-rna-detection
#7
Sze Shin Low, Hwei-San Loh, Jian Sheng Boey, Poi Sim Khiew, Wee Siong Chiu, Michelle T T Tan
An efficient electrochemical impedance genosensing platform has been constructed based on graphene/zinc oxide nanocomposite produced via a facile and green approach. Highly pristine graphene was synthesised from graphite through liquid phase sonication and then mixed with zinc acetate hexahydrate for the synthesis of graphene/zinc oxide nanocomposite by solvothermal growth. The as-synthesised graphene/zinc oxide nanocomposite was characterised with scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy and X-ray diffractometry (XRD) to evaluate its morphology, crystallinity, composition and purity...
February 27, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28301719/a-reconfigured-kennedy-pathway-which-promotes-efficient-accumulation-of-medium-chain-fatty-acids-in-leaf-oils
#8
Kyle B Reynolds, Matthew C Taylor, Darren P Cullerne, Christopher L Blanchard, Craig C Wood, Surinder P Singh, James R Petrie
Medium chain fatty acids (MCFA, C6-14 fatty acids) are an ideal feedstock for biodiesel and broader oleochemicals. In recent decades, several studies have used transgenic engineering to produce MCFA in seeds oils, though these modifications result in unbalance membrane lipid profiles that impair oil yields and agronomic performance. Given the ability to engineer non-seed organs to produce oils, we have previously demonstrated that MCFA profiles can be produced in leaves, but this also results in unbalanced membrane lipid profiles and undesirable chlorosis and cell death...
March 16, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28298838/median-and-common-peroneal-neuropathy-in-coir-workers-of-alappuzha-district-kerala
#9
Sadanandavalli Retnaswami Chandra, Biji Anand, Thomas Gregor Issac
INTRODUCTION: Coir work, in a large number of people involves mechanically rolling the coconut fibers into coir which is later subjected to various processes. The primary work is done as a cottage industry specially by women in the sitting posture for several hours. This study reports evidence of median and common peroneal neuropathy electrophysiologically in people who had been engaged in this job for several years. This study was initiated to establish the possible relationship between coir work and symptomatic neuropathies which was seen in that region with all investigations "for other causes not" contributing to the etiological diagnosis...
January 2017: Annals of Indian Academy of Neurology
https://www.readbyqxmd.com/read/28277823/the-impact-of-virgin-coconut-oil-and-high-oleic-safflower-oil-on-body-composition-lipids-and-inflammatory-markers-in-postmenopausal-women
#10
Margaret Harris, Andrea Hutchins, Lisa Fryda
This randomized crossover study compared the impact of virgin coconut oil (VCO) to safflower oil (SO) on body composition and cardiovascular risk factors. Twelve postmenopausal women (58.8 ± 3.7 year) consumed 30 mL VCO or SO for 28 days, with a 28-day washout. Anthropometrics included body weight and hip and waist circumference. Fat percent for total body, android and gynoid, fat mass, and lean mass were measured using dual-energy X-ray absorptiometry. Women maintained their typical diet recording 28 days of food records during the study...
March 9, 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/28276074/survival-of-escherichia-coli-in-common-garden-mulches-spiked-with-synthetic-greywater
#11
Staci Boyte, Sarah Quaife, Jacqui Horswell, Alma Siggins
Reuse of domestic wastewater is increasingly practiced as a means to address global demands on freshwater. Greywater is primarily reused via sub-surface irrigation of gardens, where the soil environment is seen to be an integral part of the treatment process. The fate of biological contaminants (i.e. pathogens) in the soil is reasonably well understood, but their persistence and survival in soil cover layers is largely unexplored. This study investigated the ability of Escherichia coli to survive in common soil cover layers...
March 9, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28255609/btex-emissions-from-the-largest-landfill-in-operation-in-rio-de-janeiro-brazil
#12
Priscilla Falcão de Sá Borba, Eduardo Monteiro Martins, Elisabeth Ritter, Sergio Machado Corrêa
The emission rates and the ambient air concentrations of benzene, toluene, ethyl benzene and xylenes (BTEX) were measured over the intermediate cover layer and atmosphere of the Seropédica landfill, Rio de Janeiro, Brazil. BTEX were sampled using coconut shell charcoal cartridges, followed by extraction with dichloromethane and analysis by gas chromatography with mass spectrometry. Thirteen samples were collected in areas with and without cracks in the cover layer, and six samples were collected from the ambient air...
March 2, 2017: Bulletin of Environmental Contamination and Toxicology
https://www.readbyqxmd.com/read/28245197/functional-gradients-in-the-pericarp-of-the-green-coconut-inspire-asymmetric-fibre-composites-with-improved-impact-strength-and-preserved-flexural-and-tensile-properties
#13
Nina Graupner, David Labonte, Heide Humburg, Tayfun Buzkan, Anna Dörgens, Wiebke Kelterer, Jörg Müssig
Here we investigate the mechanical properties and structural design of the pericarp of the green coconut (Cocos nucifera L.). The pericarp showed excellent impact characteristics, and mechanical tests of its individual components revealed gradients in stiffness, strength and elongation at break from the outer to the inner layer of the pericarp. In order to understand more about the potential effect of such gradients on 'bulk' material properties, we designed simple, graded, cellulose fibre-reinforced polylactide (PLA) composites by stacking layers reinforced with fibres of different mechanical properties...
February 28, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28244323/reactivity-of-free-malondialdehyde-during-in-vitro-simulated-gastrointestinal-digestion
#14
Angelique Vandemoortele, Pinar Babat, Mariam Yakubu, Bruno De Meulenaer
An aqueous buffer, a saturated glycerol triheptanoate oil, and a Tween 20 stabilized fully hydrogenated coconut oil-in-water emulsion, all spiked with malondialdehyde, were subjected to in vitro digestion. A dynamic equilibrium between malondialdehyde, its aldol self-condensation products, and its hydrolytic cleavage products was observed. This equilibrium depended upon the kind of sample and the temperature at which these samples were preincubated during 24 h. The presence of oil during gastric digestion protected the aldol self-condensation and cleavage products from conversion to malondialdehyde, which occurred in the aqueous acidic gastric chyme...
March 7, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28242932/dietary-fat-composition-food-matrix-and-relative-polarity-modulate-the-micellarization-and-intestinal-uptake-of-carotenoids-from-vegetables-and-fruits
#15
Purna Chandra Mashurabad, Ravindranadh Palika, Yvette Wilda Jyrwa, K Bhaskarachary, Raghu Pullakhandam
Dietary fat increases carotenoid bioavailability by facilitating their transfer to the aqueous micellar fraction during digestion. However, the specific effect of both quantity and type of dietary fat required for optimal carotenoid absorption remained unexplored. In the present study, the effect of amount and type of vegetable oils on carotenoid micellarization from carrot, spinach, drumstick leaves and papaya using in vitro digestion/Caco-2 cell model have been assessed. Although, dietary fat (0.5-10% w/w) significantly increased the micellarization of carotenoids from all the test foods, the extent of increase was determined by the food matrix (papaya > drumstick = spinach > carrot) and polarity of carotenoids (lutein > β-carotene = α-carotene > lycopene)...
February 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242914/defatted-coconut-residue-crude-polysaccharides-as-potential-prebiotics-study-of-their-effects-on-proliferation-and-acidifying-activity-of-probiotics-in-vitro
#16
Nur 'Ain Najwa Mohd Nor, Sahar Abbasiliasi, Mohammed Nazrim Marikkar, Arbakariya Ariff, Mehrnoush Amid, Dhilia Udie Lamasudin, Mohd Yazid Abdul Manap, Shuhaimi Mustafa
This paper reports on the extraction, partial characterization and the potential application of crude polysaccharides from defatted coconut residue as a prebiotic. The coconut residue was defatted and extracted to obtain the crude polysaccharides and its physicochemical properties were determined. The crude polysaccharides were assessed for monosaccharide composition, total carbohydrate content, reducing sugar concentration and protein content determination. The functional group and structural elucidation of crude polysaccharides was also done using Fourier transform infrared spectra analysis...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242912/oxidative-stability-thermal-stability-and-acceptability-of-coconut-oil-flavored-with-essential-oils-from-black-pepper-and-ginger
#17
Janu Chandran, N Nayana, N Roshini, P Nisha
The present study investigates the oxidative and thermal stability of flavoured oils developed by incorporating essential oils from black pepper and ginger to coconut oil (CNO) at concentrations of 0.1 and 1.0% (CNOP-0.1, CNOP-1, CNOG-0.1, CNOG-1). The stability of oils were assessed in terms of free fatty acids, peroxide, p-anisidine, conjugated diene and triene values and compared with CNO without any additives and a positive control with synthetic antioxidant TBHQ (CNOT). It was found that the stability of CNOP-1 and CNOG-1 were comparable with CNOT at both study conditions...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28242902/melting-crystallization-and-storage-stability-of-virgin-coconut-oil-and-its-blends-by-differential-scanning-calorimetry-dsc-and-fourier-transform-infrared-spectroscopy-ftir
#18
Yashi Srivastava, Anil Dutt Semwal, Vallayil Appukuttan Sajeevkumar, G K Sharma
The blends were prepared of virgin coconut oil with refined soyabean oil (VCO-RSOY) and refined safflower oil (VCO-RSAFF). Blending with VCO improved the fatty acid composition which increased the shelf stability of 20:80 VCO-RSOY and VCO-RSAFF up to 12 months in different packaging systems such as low density polyethylene, linear low density polyethylene, metalized polyester pouches, polyethylene teteraphthalate, high density polyethylene (HDPE), Amber HDPE bottle. The specific spectral regions of FTIR proved to be very useful for the determination of adulteration as well as for the study of oxidation process...
January 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28240186/plasma-potassium-levels-in-healthy-prehypertension-subjects-and-the-role-of-a-high-potassium-drink
#19
Farapti Farapti, Savitri Sayogo, Parlindungan Siregar
Most populations around the world consume less than the recommended levels of potassium. Long term low potassium intake could lead to decreased plasma potassium levels and induce hypokalemia. The increasing of plasma potassium levels 0,2-0,4 mmol/L by improving potassium intake decreased significantly blood pressure (BP). Assessing plasma potassium levels in healthy people related to potassium intake have not been studied. In this study, we analysed plasma potassium levels in prehypertension (PHT) subjects and to evaluate the effect of tender coconut water (TCW) as a high potassium drink on plasma potassium levels in PHT adults...
February 24, 2017: Current Hypertension Reviews
https://www.readbyqxmd.com/read/28221944/a-novel-immunoassay-test-system-for-detection-of-modified-allergen-residues-present-in-almond-cashew-coconut-hazelnut-and-soy-based-nondairy-beverages
#20
Jongkit Masiri, Lora Benoit, Mahzad Meshgi, Jeffrey Day, Cesar Nadala, Mansour Samadpour
A growing number of plant-based milk substitutes have become commercially available, providing an array of options for consumers with dietary restrictions. Though several of these products rival cow's milk in terms of their nutritional profiles, beverages prepared with soy and tree nuts can be a significant concern to consumers because of potential contamination with food allergens. Adding to this concern is the fact that allergen residues from plant-based beverages are modified during manufacturing, thereby decreasing the sensitivity of antibody-based detection methods...
September 2016: Journal of Food Protection
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