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https://www.readbyqxmd.com/read/28528505/enhanced-degradation-of-perfluorooctanoic-acid-using-dielectric-barrier-discharge-with-la-ce-doped-tio2
#1
Shanping Li, Xiaoping Wang, Lijun Liu, Yongbo Guo, Qinglin Mu, Abdelwahid Mellouki
A synergistic system of dielectric barrier discharge (DBD) combined with La/Ce-TiO2 was developed to investigate the decomposition performance of the environmentally persistent perfluorooctanoic acid (PFOA). The La/Ce-TiO2 was modified by sol-gel method and characterized by XRD, SEM, and energy dispersive X-ray. The effects of PFOA concentration, applied voltage, initial pH, liquid conductivity, and additives on the removal rate of PFOA were explored. The results showed that the La/Ce-TiO2 exhibited excellent catalytic effects on PFOA degradation in DBD system...
May 20, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28528433/hydrolysis-of-phosphatidylcholine-isoprostanes-ptdcho-ip-by-peripheral-human-group-iia-v-and-x-secretory-phospholipases-a2-spla2
#2
Arnis Kuksis, Waldemar Pruzanski
Biologically active F- and E/D-type-prostane ring isomers (F2-IP and E2/D2-IP, respectively) are produced in situ by non-enzymatic peroxidation of arachidonic acid esterified to GroPCho (PtdCho-IP) and are universally distributed in tissue lipoproteins and cell membranes. Previous work has shown that platelet-activating factor acetylhydrolases (PAF-AH) are the main endogenous PLA2 involved in degradation of PtdCho-IP. The present study shows that the PtdCho-IP are also subject to hydrolysis by group IIA, V and X secretory PLA2, which also have a wide peripheral tissue distribution...
May 20, 2017: Lipids
https://www.readbyqxmd.com/read/28528177/tetrodotoxin-in-asian-newts-salamandridae
#3
Mari Yotsu-Yamashita, Stefan W Toennes, Dietrich Mebs
Tetrodotoxin (TTX) and its analogues occur in a wide range of marine animals but also in terrestrial vertebrates such as frogs, toads and newts. Despite numerous studies on TTX in New World newts (Notophthalmus viridescens, Taricha spp.), few data only exist for Asian newts. Methanolic extracts of newts from China (Cynops orientalis, Pachytriton labiatus, Paramesotriton chinensis), Vietnam (Paramesotriton deloustali, P. guangxiensis), and Laos (Laotriton laoensis) were analyzed by liquid-chromatography-fluorescent detection (LC-FLD) and mass-spectrometry (LC-MS)...
May 17, 2017: Toxicon: Official Journal of the International Society on Toxinology
https://www.readbyqxmd.com/read/28528097/protein-digestomic-analysis-reveals-the-bioactivity-of-deer-antler-velvet-in-simulated-gastrointestinal-digestion
#4
Yang Yu, Yan Jin, Fangjun Wang, Jiaze Yan, Yanxia Qi, Mingliang Ye
Proteins are the most prominent bioactive component in deer antler velvet. The aim of the present study was to track the fate of protein of antler velvet by protein digestomics. The peptide profile identified by LC-MS/MS and the in vitro bioactivity of antler velvet aqueous extract (AAE) were investigated in simulated gastrointestinal digestion. A total of 23, 387 and 417 peptides in AAE, gastric and pancreatic digests were identified using LC-MS/MS, respectively. Collagens, the predominant proteins, released 34 peptides in gastric digests and 146 peptides in pancreatic digests...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28527949/microproteome-of-dentoalveolar-tissues
#5
Cristiane R Salmon, Ana Paula O Giorgetti, Adriana F Paes Leme, Romênia R Domingues, Tamara N Kolli, Brian L Foster, Francisco H Nociti
Proteomic analysis of extracellular matrices (ECM) of dentoalveolar tissues can provide insights into developmental, pathological, and reparative processes. However, targeted dissection of mineralized tissues, dental cementum (DC), alveolar bone (AB), and dentin (DE), presents technical difficulties. We demonstrate an approach combining EDTA decalcification and laser capture microdissection (LCM), followed by liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), to analyze proteome profiles of these tissues...
May 17, 2017: Bone
https://www.readbyqxmd.com/read/28527785/the-effectiveness-of-a-fixed-dose-combination-pour-on-formulation-of-1-25-fipronil-and-2-5-fluazuron-against-economically-important-ectoparasites-and-associated-pharmacokinetics-in-cattle
#6
Welber Daniel Zanetti Lopes, Rafael Marin Chiummo, Luis Fernando Vettorato, Daniel de Castro Rodrigues, Rafael Bazaglia Sonada
The present work consisted of eight studies to evaluate the ectoparasiticidal spectrum and determine the pharmacokinetic parameters of a pour-on combination of fipronil 1.25mg/kg+fluazuron 2.5mg/kg for cattle against Rhipicephalus microplus, Haematobia irritans and the larvae of Dermatobia hominis and Cochliomyia hominivorax. The analysis fipronil and fluazuron were performed by liquid chromatography using a mass detector for the detection and quantification of analytes (LC-MS/MS). Additionally, in two of these studies, the animals were artificially infested with R...
May 17, 2017: Parasitology International
https://www.readbyqxmd.com/read/28527513/enzymatic-biosynthesis-of-novel-neobavaisoflavone-glucosides-via-bacillus-udp-glycosyltransferase
#7
Tao Ma, Yi-Qun Dai, Nan Li, Qiang Huo, Hong-Mei Li, Yu-Xin Zhang, Zheng-Hao Piao, Cheng-Zhu Wu
The present study was designed to perform structural modifications of of neobavaisoflavone (NBIF), using an in vitro enzymatic glycosylation reaction, in order to improve its water-solubility. Two novel glucosides of NBIF were obtained from an enzymatic glycosylation by UDP-glycosyltransferase. The glycosylated products were elucidated by LC-MS, HR-ESI-MS, and NMR analysis. The HPLC peaks were integrated and the concentrations in sample solutions were calculated. The MTT assay was used to detect the cytotoxic activity of compounds in cancer cell lines...
April 2017: Chinese Journal of Natural Medicines
https://www.readbyqxmd.com/read/28527417/fate-of-the-sunscreen-compound-oxybenzone-in-cyperus-alternifolius-based-hydroponic-culture-uptake-biotransformation-and-phytotoxicity
#8
Feiran Chen, Christian Huber, Peter Schröder
Oxybenzone (OBZ), a common ingredient in sunscreens and personal care products, has been frequently detected in effluents from municipal wastewater treatment plants and also in surface waters. OBZ is an emerging contaminant due to its adverse impacts on marine/aquatic ecosystems. To investigate the removal and degradation capacity of phytotreatment for OBZ, the common wetland plant species Cyperus alternifolius L. was exposed to this compound at 5, 25 and 50 μM for 120 h, respectively. Continuous uptake by roots and accumulation in plant tissues was observed over the exposure time, and depletion of spiked OBZ from the aqueous medium exceeded 73...
May 12, 2017: Chemosphere
https://www.readbyqxmd.com/read/28527184/polyphenols-as-possible-markers-of-botanical-origin-of-honey
#9
Uroš M Gašić, Dušanka M Milojković-Opsenica, Živoslav Lj Tešić
In recent years, the botanical and geographical origin of food has become an important topic in the context of food quality and safety, as well as consumer protection, in accordance with international standards. Finding chemical markers, especially phytochemicals, characteristic for some kind of food is the subject of interest of a significant number of researchers in the world. This paper is focused on the use of polyphenols as potential markers for the determination of botanical origin of honey. It includes a review of the polyphenols present in various honey samples and the methods for their separation and identification...
May 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28527020/changes-in-serum-levels-of-kynurenine-metabolites-in-paediatric-patients-affected-by-adhd
#10
Melania Evangelisti, Pietro De Rossi, Jole Rabasco, Renato Donfrancesco, Luana Lionetto, Matilde Capi, Gabriele Sani, Maurizio Simmaco, Ferdinando Nicoletti, Maria Pia Villa
This study aims at determining serum levels of tryptophan and other metabolites of the kynurenine pathway in children with attention deficit hyperactivity disorder (ADHD) compared to healthy controls. Such metabolites interact with glutamate receptors in the central nervous system, potentially modulating mechanisms that are pivotal in ADHD and thus potentially representing peripheral biomarkers of the disorder. We measured serum levels of tryptophan and some metabolites of the kynurenine pathway in 102 children with ADHD and 62 healthy controls by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
May 19, 2017: European Child & Adolescent Psychiatry
https://www.readbyqxmd.com/read/28526811/proteomic-analysis-of-cerebrospinal-fluid-from-children-with-central-nervous-system-tumors-identifies-candidate-proteins-relating-to-tumor-metastatic-spread
#11
Filippo Spreafico, Italia Bongarzone, Sara Pizzamiglio, Ruben Magni, Elena Taverna, Maida De Bortoli, Chiara M Ciniselli, Elena Barzanò, Veronica Biassoni, Alessandra Luchini, Lance A Liotta, Weidong Zhou, Michele Signore, Paolo Verderio, Maura Massimino
Central nervous system (CNS) tumors are the most common solid tumors in childhood. Since the sensitivity of combined cerebrospinal fluid (CSF) cytology and radiological neuroimaging in detecting meningeal metastases remains relatively low, we sought to characterize the CSF proteome of patients with CSF tumors to identify biomarkers predictive of metastatic spread. CSF samples from 27 children with brain tumors and 13 controls (extra-CNS non-Hodgkin lymphoma) were processed using core-shell hydrogel nanoparticles, and analyzed with reverse-phase liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS)...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28525790/quantitative-lc-hrms-determination-of-selected-cardiovascular-drugs-in-dried-blood-spots-as-an-indicator-of-adherence-to-medication
#12
Dennis Bernieh, Graham Lawson, Sangeeta Tanna
Dried blood spot (DBS) sampling was investigated as a means of obtaining micro-volume blood samples for the quantitative analyses of ten commonly UK prescribed cardiovascular drugs as an indicator of medication adherence. An 8mm disc was punched out from each DBS from calibration, quality control and volunteer samples and extracted using methanol containing the internal standard. Each extract was evaporated to dryness, the residue reconstituted in methanol:water (40:60v/v) containing 0.1% formic acid and analysed by LC-HRMS...
May 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28525713/identification-and-validation-of-sensory-active-compounds-from-data-driven-research-a-flavoromics-approach
#13
Ian Ronningen, Michelle Miller, Youlin Xia, Devin G Peterson
In the current study significant LC/MS features, from multivariate analysis (MVA) models developed using untargeted chemical fingerprinting to characterize flavor changes in extracts of citrus fruits related to aging, were further isolated and analyzed for sensory relevant information, followed by structural elucidation of flavor actives. The top ten statistical features from two MVA approaches, partial least squares data analysis (PLS-DA) and Random Forrest (RF), were purified to approximately 70% via multi-dimensional liquid chromatography-mass directed fractionation to screen for sensory activity...
May 19, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28525384/secretome-analysis-of-breast-cancer-associated-adipose-tissue-to-identify-paracrine-regulators-of-breast-cancer-growth
#14
Lapeire Lore, Hendrix An, Lecoutere Evelyne, Van Bockstal Mieke, Vandesompele Jo, Maynard Dawn, Braems Geert, Van Den Broecke Rudy, Müller Cathérine, Bracke Marc, Cocquyt Véronique, Denys Hannelore, De Wever Olivier
Adipose tissue secretes a plethora of adipokines as evidenced by characterization of subcutaneous and visceral adipose tissue secretomes. However, adipose tissue composition and secretion pattern is depot and disease dependent, influencing the adipose tissue secretome. We investigated the secretome of cancer-associated adipose tissue (CAAT) explants from breast cancer patients and explored its role in breast cancer proliferation. CAAT proteins were identified by LC-MS/MS and human protein antibody arrays and stimulated proliferation of three breast cancer cell lines...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28524783/quantitative-analysis-of-veterinary-drugs-in-bovine-muscle-and-milk-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#15
Shizuka Saito-Shida, Takatoshi Sakai, Satoru Nemoto, Hiroshi Akiyama
A simple and reliable multiresidue method for quantitative determination of veterinary drugs in bovine muscle and milk using liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was developed. Critical MS parameters such as capillary voltage, cone voltage, collision energy, desolvation gas temperature, and extraction mass window were carefully optimised to obtain the best possible sensitivity. Analytical samples were prepared using extraction with acetonitrile and hexane in the presence of anhydrous sodium sulphate and acetic acid, followed by ODS cartridge clean-up...
May 19, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28524240/potential-serum-biomarkers-associated-with-mild-and-severe-leptospirosis-infection-a-cohort-study-in-the-malaysian-population
#16
Tan Xue Ting, Fairuz Binti Amran, Ravindran Thayan, Norazah Ahmad, Roslinda Jaafar, Rahimah Haron, Rafidah Abdullah, Sazwan Reezal Bin Shamsuddin, Nor Suhaila Binti Md Riffin, Puteri Shafinaz Abdul-Rahman
Leptospirosis is an emerging zoonotic infectious disease in Malaysia. The symptoms of leptospirosis vary from mild non-specific flu-like illness to a severe condition which is usually associated with serious complication and fatality. To study the protein expression profile of mild and severe leptospirosis, fifteen paired sera were collected from the patients who were mildly infected and following that progressed to severe stage. The proteome profiles of mild and severe cases were studied using 2DE analysis in combination with LC-MS/MS...
May 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28524101/development-of-a-lc-ms-ms-method-for-the-multi-mycotoxin-determination-in-composite-cereal-based-samples
#17
Barbara De Santis, Francesca Debegnach, Emanuela Gregori, Simona Russo, Francesca Marchegiani, Gabriele Moracci, Carlo Brera
The analytical scenario for determining contaminants in the food and feed sector is constantly prompted by the progress and improvement of knowledge and expertise of researchers and by the technical innovation of the instrumentation available. Mycotoxins are agricultural contaminants of fungal origin occurring at all latitudes worldwide and being characterized by acute and chronic effects on human health and animal wellness, depending on the species sensitivity. The major mycotoxins of food concern are aflatoxin B1 and ochratoxin A, the first for its toxicity, and the second for its recurrent occurrence...
May 18, 2017: Toxins
https://www.readbyqxmd.com/read/28523741/comparative-membrane-proteomics-analyses-of-breast-cancer-cell-lines-to-understand-the-molecular-mechanism-of-breast-cancer-brain-metastasis
#18
Wenjing Peng, Yu Zhang, Rui Zhu, Yehia Mechref
Breast cancer is the leading type of cancer in women. Breast cancer brain metastasis is currently considered an issue of concern among breast cancer patients. Membrane proteins play important roles in breast cancer brain metastasis, involving cell adhesion and penetration of blood-brain barrier. To understand the mechanism of breast cancer brain metastasis, liquid chromatography-tandem mass spectrometry (LC-MS/MS) was employed in conjunction with enrichment of membrane proteins to analyze the proteomes from 5 different breast cancer and a brain cancer cell lines...
May 19, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28523515/distribution-of-exogenous-and-endogenous-cyp3a-markers-and-related-factors-in-healthy-males-and-females
#19
Jieon Lee, Andrew HyoungJin Kim, SoJeong Yi, SeungHwan Lee, Seo Hyun Yoon, Kyung-Sang Yu, In-Jin Jang, Joo-Youn Cho
Cytochrome P450 (CYP) 3A is an important drug-metabolizing enzyme in humans. Assessing CYP3A activity is necessary for predicting therapeutic outcomes or the potential adverse events of various therapeutics. This study sought to evaluate the distribution of endogenous and exogenous markers reflecting hepatic CYP3A activity and related factors affecting its activity in healthy male and female. Each subject was given a single 1 mg dose of midazolam intravenously. Pharmacokinetics, pharmacometabolomics, and pharmacogenomics analyses were performed to evaluate CYP3A activity...
May 18, 2017: AAPS Journal
https://www.readbyqxmd.com/read/28523434/salivary-exosomal-psma7-a-promising-biomarker-of-inflammatory-bowel-disease
#20
Xiaowen Zheng, Feng Chen, Qian Zhang, Yulan Liu, Peng You, Shan Sun, Jiuxiang Lin, Ning Chen
Inflammatory bowel disease (IBD) is an intestinal immune-dysfunctional disease worldwide whose prevalence increasing in Asia including China. It is a chronic disease of the gastrointestinal tract with unknown cause. Exosomes are small vesicles in various body fluids. They have diameters of 40-120 nm, and one of their functions is long-distance transfer of various substances. In this study, we investigated the contents of salivary exosomes in patients with IBD and in healthy controls to explore a new biomarker in patients with IBD...
May 18, 2017: Protein & Cell
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