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https://www.readbyqxmd.com/read/27923200/a-quantitative-lc-ms-ms-method-for-simultaneous-determination-of-cocaine-and-its-metabolites-in-whole-blood
#1
Xiabin Chen, Xirong Zheng, Kai Ding, Ziyuan Zhou, Chang-Guo Zhan, Fang Zheng
As new metabolic pathways of cocaine were recently identified, a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed to simultaneously determine cocaine and nine cocaine-related metabolites in whole blood samples. One-step solid phase extraction was used to extract all of the ten compounds and corresponding internal standards from blood samples. All compounds and internal standards extracted were separated on an Atlantis T3 (100Å, 3μm, 2.1mm×150mm I.D) column and detected in positive ion and high sensitivity mode with multiple reaction monitoring...
November 11, 2016: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/27921458/metabolic-profile-of-human-coelomic-fluid
#2
C Virgiliou, L Valianou, M Witting, F Moritz, C Fotakis, P Zoumpoulakis, A C Chatziioannou, L Lazaros, G Makrydimas, K Chatzimeletiou, N Raikos, G A Theodoridis
AIM: Till now there is very limited knowledge on the molecular content of coelomic fluid and cells. This study presents the first attempt to elucidate the metabolic profile of such samples. METHODOLOGY: Samples were collected via coelocentesis from 41 women during the first trimester of gestation. Metabolic content was assessed using four different analytical platforms. For targeted analysis a hydrophilic interaction chromatography ultra high performance LC-MS/MS method was applied...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27921457/impact-of-exercise-on-fecal-and-cecal-metabolome-over-aging-a-longitudinal-study-in-rats
#3
Olga Deda, Helen Gika, Theodoros Panagoulis, Ioannis Taitzoglou, Nikolaos Raikos, Georgios Theodoridis
AIM: Physical exercise can reduce adverse conditions during aging, while both exercise and aging act as metabolism modifiers. The present study investigates rat fecal and cecal metabolome alterations derived from exercise during rats' lifespan. METHODS & RESULTS: Groups of rats trained life-long or for a specific period of time were under study. The training protocol consisted of swimming, 15-18 min per day, 3-5 days per week, with load of 4-0% of rat's weight. Fecal samples and cecal extracts were analyzed by targeted and untargeted metabolic profiling methods (GC-MS and LC-MS/MS)...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27921456/capillary-electrophoresis-mass-spectrometry-as-a-tool-for-untargeted-metabolomics
#4
Antonia García, Joanna Godzien, Ángeles López-Gonzálvez, Coral Barbas
Highly polar and ionic metabolites, such as sugars, most amino acids, organic acids or nucleotides are not retained by conventional reversed-phase LC columns and polar stationary phases and hydrophilic-interaction LC lacks of robustness, which is still limiting their applications for untargeted metabolomics where reproducibility is a must. Biological samples such as blood, urine or even tissues include many hydrophilic compounds secreted from cells, their analysis is essential for biomarker discovery, disease progression or treatment effects...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27921453/integrating-ion-mobility-spectrometry-into-mass-spectrometry-based-exposome-measurements-what-can-it-add-and-how-far-can-it-go
#5
Thomas O Metz, Erin S Baker, Emma L Schymanski, Ryan S Renslow, Dennis G Thomas, Tim J Causon, Ian K Webb, Stephan Hann, Richard D Smith, Justin G Teeguarden
Measuring the exposome remains a challenge due to the range and number of anthropogenic molecules that are encountered in our daily lives, as well as the complex systemic responses to these exposures. One option for improving the coverage, dynamic range and throughput of measurements is to incorporate ion mobility spectrometry (IMS) into current MS-based analytical methods. The implementation of IMS in exposomics studies will lead to more frequent observations of previously undetected chemicals and metabolites...
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27921451/targeted-full-scan-lc-ms-metabolomics-simultaneous-quantitation-of-knowns-and-feature-discovery-provide-the-best-of-both-worlds
#6
Adam P Rosebrock
No abstract text is available yet for this article.
January 2017: Bioanalysis
https://www.readbyqxmd.com/read/27921366/masked-trichothecene-and-zearalenone-mycotoxins-withstand-digestion-and-absorption-in-the-upper-gi-tract-but-are-efficiently-hydrolyzed-by-human-gut-microbiota-in-vitro
#7
Silvia W Gratz, Reshma Dinesh, Tomoya Yoshinari, Grietje Holtrop, Anthony J Richardson, Gary Duncan, Susan MacDonald, Antony Lloyd, Jonathan Tarbin
SCOPE: Cereal grains are commonly contaminated with Fusarium mycotoxins and their plant-derived masked metabolites. The fate of masked mycotoxins in the human gut is poorly understood. Here we assess the metabolism and transport of glucoside metabolites of common trichothecenes (deoxynivalenol, nivalenol, T-2 toxin) and zearalenone compounds (zearalenone, α- and β-zearalenol) in the human gut in vitro. METHODS AND RESULTS: Masked mycotoxins were incubated with artificial digestive juices and absorption was assessed in differentiated Caco-2/TC7 cells...
December 6, 2016: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/27921079/data-file-of-a-deep-proteome-analysis-of-the-prefrontal-cortex-in-aged-mice-with-progranulin-deficiency-or-neuronal-overexpression-of-progranulin
#8
Juliana Heidler, Stefanie Hardt, Ilka Wittig, Irmgard Tegeder
Progranulin deficiency is associated with neurodegeneration in humans and in mice. The mechanisms likely involve progranulin-promoted removal of protein waste via autophagy. We performed a deep proteomic screen of the pre-frontal cortex in aged (13-15 months) female progranulin-deficient mice (GRN(-/-)) and mice with inducible neuron-specific overexpression of progranulin (SLICK-GRN-OE) versus the respective control mice. Proteins were extracted and analyzed per liquid chromatography/mass spectrometry (LC/MS) on a Thermo Scientific™ Q Exactive Plus equipped with an ultra-high performance liquid chromatography unit and a Nanospray Flex Ion-Source...
December 2016: Data in Brief
https://www.readbyqxmd.com/read/27920753/neuroproteomics-and-systems-biology-approach-to-identify-temporal-biomarker-changes-post-experimental-traumatic-brain-injury-in-rats
#9
Firas H Kobeissy, Joy D Guingab-Cagmat, Zhiqun Zhang, Ahmed Moghieb, Olena Y Glushakova, Stefania Mondello, Angela M Boutté, John Anagli, Richard Rubenstein, Hisham Bahmad, Amy K Wagner, Ronald L Hayes, Kevin K W Wang
Traumatic brain injury (TBI) represents a critical health problem of which diagnosis, management, and treatment remain challenging. TBI is a contributing factor in approximately one-third of all injury-related deaths in the United States. The Centers for Disease Control and Prevention estimate that 1.7 million people suffer a TBI in the United States annually. Efforts continue to focus on elucidating the complex molecular mechanisms underlying TBI pathophysiology and defining sensitive and specific biomarkers that can aid in improving patient management and care...
2016: Frontiers in Neurology
https://www.readbyqxmd.com/read/27920527/pharmacokinetics-and-safety-of-dts-108-a-human-oligopeptide-bound-to-sn-38-with-an-esterase-sensitive-cross-linker-in-patients-with-advanced-malignancies-a-phase-i-study
#10
Romain Coriat, Sandrine J Faivre, Olivier Mir, Chantal Dreyer, Stanislas Ropert, Mohammed Bouattour, Robert Desjardins, François Goldwasser, Eric Raymond
BACKGROUND: DTS-108 is a hydrosoluble prodrug, where the SN-38 moiety is covalently linked to a 20-amino acid vector peptide by a specific esterase-sensitive cross-linker, releasing 7-ethyl-10-hydroxycampthotecin (SN-38) by esterase bond cleavage. METHODS: The pharmacokinetics of DTS-108, adverse events graded according to NCI-CTCv3.1, dose-limiting toxicities at cycle 1, the maximum tolerated dose (MTD), and the recommended Phase II dose (RP2D) of intravenous DTS-108 (1-2 hours) every 2 weeks were evaluated in a first-in-human Phase I study in patients with advanced/metastatic carcinomas, according to an accelerated dose escalation design...
2016: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/27919896/cephalosporin-3-diazeniumdiolate-no-donor-prodrug-pyrro-c3d-enhances-azithromycin-susceptibility-of-non-typeable-haemophilus-influenzae-biofilms
#11
Samuel A Collins, Michael J Kelso, Ardeshir Rineh, Nageshwar R Yepuri, Janice Coles, Claire L Jackson, Georgia D Halladay, Woolf T Walker, Jeremy S Webb, Luanne Hall-Stoodley, Gary J Connett, Martin Feelisch, Saul N Faust, Jane S A Lucas, Raymond N Allan
OBJECTIVES: PYRRO-C3D is a cephalosporin-3-diazeniumdiolate nitric oxide (NO)-donor prodrug designed to selectively deliver NO to bacterial infection sites. The objective of this study was to assess the activity of PYRRO-C3D against non-typeable Haemophilus influenzae (NTHi) biofilms and examine the role of NO in reducing biofilm-associated antibiotic tolerance. METHODS: The activity of PYRRO-C3D on in vitro NTHi biofilms was assessed through CFU enumeration and confocal microscopy...
December 5, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27919805/uplc-ms-for-metabolomics-a-giant-step-forward-in-support-of-pharmaceutical-research
#12
REVIEW
Ala F Nassar, Terence Wu, Samuel F Nassar, Adam V Wisnewski
Metabolomics is a relatively new and rapidly growing area of post-genomic biological research. As use of metabolomics technology grows throughout the spectrum of drug discovery and development, and its applications broaden, its impact is expanding dramatically. This review seeks to provide the reader with a brief history of the development of metabolomics, its significance and strategies for conducting metabolomics studies. The most widely used analytical tools for metabolomics: NMR, LC-MS and GC-MS, are discussed along with considerations for their use...
December 2, 2016: Drug Discovery Today
https://www.readbyqxmd.com/read/27919190/investigation-of-the-influence-of-glycyrrhizin-on-the-pharmacokinetics-of-celastrol-in-rats-using-lc-ms-and-its-potential-mechanism
#13
Guangkui Yan, Hanhua Zhang, Wei Wang, Yuan Li, Chenghuang Mao, Mingqiao Fang, Xianhong Yi, Jingdong Zhang
1. The aim of this study was to investigate the effects of glycyrrhizin on the pharmacokinetics of celastrol in rats. 2. Twelve male Sprague-Dawley rats were randomly assigned to two groups: control group and test group. Test group was pretreated with glycyrrhizin at a dose of 100 mg/kg/day for 10 days, and then the two groups were orally administered with celastrol at a dose of 1 mg/kg. The concentration of celastrol was determined using a sensitive and reliable LC-MS method. 3. The results showed that glycyrrhizin could significantly decrease the plasma concentration (from 64...
December 5, 2016: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
https://www.readbyqxmd.com/read/27916965/pathway-analysis-and-metabolites-identification-by-metabolomics-of-etiolation-substrate-from-fresh-cut-chinese-water-chestnut-eleocharis-tuberosa
#14
Yi-Xiao Li, Yong-Gui Pan, Feng-Ping He, Meng-Qi Yuan, Shang-Bin Li
Fresh-cut Chinese water chestnuts (CWC) turn yellow after being peeled, reducing their shelf life and commercial value. Metabolomics, the systematic study of the full complement of small molecular metabolites, was useful for clarifying the mechanism of fresh-cut CWC etiolation and developing methods to inhibit yellowing. In this study, metabolic alterations associated with etiolation at different growth stages (0 day, 2 days, 3 days, 4 days, 5 days) from fresh-cut CWC were investigated using LC-MS and analyzed by pattern recognition methods (principal component analysis (PCA), partial least squares-discriminant analysis (PLS-DA), and orthogonal projection to latent structures-discriminant analysis (OPLS-DA))...
December 1, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27916945/cytokinins-and-expression-of-sweet-sut-cwinv-and-aap-genes-increase-as-pea-seeds-germinate
#15
Paula E Jameson, Pragatheswari Dhandapani, Ondrej Novak, Jiancheng Song
Transporter genes and cytokinins are key targets for crop improvement. These genes are active during the development of the seed and its establishment as a strong sink. However, during germination, the seed transitions to being a source for the developing root and shoot. To determine if the sucrose transporter (SUT), amino acid permease (AAP), Sugar Will Eventually be Exported Transporter (SWEET), cell wall invertase (CWINV), cytokinin biosynthesis (IPT), activation (LOG) and degradation (CKX) gene family members are involved in both the sink and source activities of seeds, we used RT-qPCR to determine the expression of multiple gene family members, and LC-MS/MS to ascertain endogenous cytokinin levels in germinating Pisum sativum L...
December 1, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27916855/comparative-proteome-analysis-of-porcine-jejunum-tissues-in-response-to-a-virulent-strain-of-porcine-epidemic-diarrhea-virus-and-its-attenuated-strain
#16
Zhonghua Li, Fangzhou Chen, Shiyi Ye, Xiaozhen Guo, Atta Muhanmmad Memon, Meizhou Wu, Qigai He
Porcine epidemic diarrhea virus (PEDV), a predominant cause of acute enteric infection, leads to severe dehydrating diarrhea and mortality in piglets all over the world. A virulent PEDV YN13 strain, isolated in our laboratory, was attenuated to yield an attenuated PEDV strain YN144. To better understand the pathogenesis mechanism and the virus-host interaction during infection with both PEDV YN13 and YN144 strains, a comparative proteomic analysis was carried out to investigate the proteomic changes produced in the primary target organ, using isobaric tags for relative and absolute quantitation (iTRAQ) labeling, followed by liquid chromatography tandem-mass spectrometry (LC-MS/MS)...
November 29, 2016: Viruses
https://www.readbyqxmd.com/read/27916587/genetic-toxicity-of-epimedium-koreanum-nakai
#17
Youn-Hwan Hwang, Hey Jin Yang, Nam-Hui Yim, Jin Yeul Ma
ETHNOPHARMACOLOGICAL RELEVANCE: In Eastern Asia, E. koreanum Nakai (EKN) has traditionally been used as an aphrodisiac herbal medicine. However, there was no available information for its genotoxicity. This study was conducted to evaluate the genotoxic potentials of EKN. MATERIALS AND METHODS: The phytochemicals of EKN were identified using liquid chromatography/tandem mass spectrometry (LC/MS/MS). Three standard battery of genotoxicity assay for bacterial reverse mutation, mammalian chromosomal aberration and in vivo micronuclei formation was employed...
December 1, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/27916133/the-use-of-a-restricted-access-volatile-supramolecular-solvent-for-the-lc-ms-ms-assay-of-bisphenol-a-in-urine-with-a-significant-reduction-of-phospholipid-based-matrix-effects
#18
José Ángel Salatti-Dorado, Noelia Caballero-Casero, María Dolores Sicilia, María Loreto Lunar, Soledad Rubio
Restricted access-volatile supramolecular solvents (RAM-VOL-SUPRAS) are here proposed as a new strategy for the quick removal of protein and phospholipids and efficient analyte extraction in LC-MS bioanalysis. Quantification of bisphenol A in urine was selected to prove the suitability of this approach for the intended purpose. RAM-VOL-SUPRAS were spontaneously synthesized in urine by addition of hexanol (83 μL) dissolved in THF (150 μL). SUPRAS composition was environment-dependent and an equation for prediction of SUPRAS volume under given experimental conditions was proposed...
January 15, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27915587/the-determination-of-testosterone-esters-and-estradiol-esters-in-bovine-and-porcine-blood-serum
#19
Martina Rejtharová, Libor Rejthar, Katarína Čačková
Monitoring of steroid esters in blood serum is desirable in order to detect possible illegal use of natural hormones as growth promoters. A method for determination of testosterone propionate, testosterone benzoate, testosterone isocaproate, testosterone decanoate and estradiol benzoate in bovine and porcine blood serum was developed. The procedure consists of protein precipitation and removal of phospholipids using a HybridSPE®-Phospholipid column followed by clean-up on a hydrophilic modified styrene polymer Supel(TM) -Select HLB column and LC-MS/MS measurement...
December 5, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/27915584/single-laboratory-validation-of-an-lc-ms-ms-method-for-determining-florfenicol-ff-and-florfenicol-amine-ffa-residues-in-chicken-feathers-and-application-to-a-residue-depletion-study
#20
J Cornejo, E Pokrant, R Riquelme, C Briceño, A Maddaleno, C Araya-Jordán, B San Martin
A suitable analytical method is required to study the behaviour of florfenicol (FF) and its metabolite florfenicol amine (FFA) in broiler's feathers. An LC-MS/MS method was developed, assessed, and intralaboratory-validated for FF and FFA analyses. We chose cloramphenicol-d5 as an internal standard, acetone as a solvent for the extraction of the analytes and dichloromethane for the clean-up. Through LC-MS/MS analysis, we established a detection limit of 20 μg kg(-1), as well as a calculated quantification limit of 24...
December 5, 2016: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
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