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https://www.readbyqxmd.com/read/28732012/multi-approach-metabolomics-analysis-and-artificial-simplified-phytocomplexes-reveal-cultivar-dependent-synergy-between-polyphenols-and-ascorbic-acid-in-fruits-of-the-sweet-cherry-prunus-avium-l
#1
Mauro Commisso, Martino Bianconi, Flavia Di Carlo, Stefania Poletti, Alessandra Bulgarini, Francesca Munari, Stefano Negri, Matteo Stocchero, Stefania Ceoldo, Linda Avesani, Michael Assfalg, Gianni Zoccatelli, Flavia Guzzo
Fruits of the sweet cherry (Prunus avium L.) accumulate a range of antioxidants that can help to prevent cardiovascular disease, inflammation and cancer. We tested the in vitro antioxidant activity of 18 sweet cherry cultivars collected from 12 farms in the protected geographical indication region of Marostica (Vicenza, Italy) during two growing seasons. Multiple targeted and untargeted metabolomics approaches (NMR, LC-MS, HPLC-DAD, HPLC-UV) as well as artificial simplified phytocomplexes representing the cultivars Sandra Tardiva, Sandra and Grace Star were then used to determine whether the total antioxidant activity reflected the additive effects of each compound or resulted from synergistic interactions...
2017: PloS One
https://www.readbyqxmd.com/read/28731694/the-foliar-desiccators-glyphosate-carfentrazone-and-paraquat-affect-the-technological-and-chemical-properties-of-cowpea-grains
#2
Igor da Silva Lindemann, Gustavo Heinrich Lang, Jessica Fernanda Hoffmann, Cesar Valmor Rombaldi, Mauricio de Oliveira, Moacir Cardoso Elias, Nathan Levien Vanier
The effects of the use of glyphosate (GLY), glyphosate plus carfentrazone (GLY/CAR), and paraquat (PAR) as plant desiccators on the technological and chemical properties of cowpea grains were investigated. All studied desiccants provided lower cooking time to freshly harvested cowpea. However, the coat color of PAR- and GLY/CAR-treated cowpea was reddish than control treatment. Principal component analysis (PCA) from liquid chromatography-mass spectrometry (LC-MS) data sets showed a clear distinction among cowpea from the different treatments...
July 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28731579/residual-dynamic-and-risk-assessment-of-dimethomorph-in-swiss-chard-grown-in-two-different-sites
#3
Md Humayun Kabir, A M Abd El-Aty, Md Musfiqur Rahman, Hyung Suk Chung, Han Sol Lee, Mi-Ra Kim, Byung-Joon Chang, Jing Wang, Ho-Chul Shin, Jae-Han Shim
Residue analysis of dimethomorph in Swiss chard cultivated at two different locations under greenhouse conditions was conducted using high-performance liquid chromatography-ultraviolet detector (HPLC-UVD) and confirmed by tandem mass spectrometry (LC-MS/MS). The randomly collected samples (over 14 days) were extracted with acetonitrile and purified using a Florisil solid-phase extraction (SPE) cartridge. Linearity over a concentration range of 0.05-50.0 mg/L had an excellent coefficient of determination (R(2) ) = 0...
July 21, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/28731312/gentiana-kurroo-royle-attenuates-the-metabolic-aberrations-in-diabetic-rats-swertiamarin-swertisin-and-lupeol-being-the-possible-bioactive-principles
#4
Khalid Ghazanfar, Khan Mubashir, Showkat A Dar, Tazeen Nazir, Iqra Hameed, Bashir A Ganai, Seema Akbar, Akbar Masood
Background Gentiana kuroo Royle is a medicinally important plant of north-western Himalayas used for various ailments. In the present study, the plant extracts were investigated for the antidiabetic effects in streptozotocin-induced diabetic rats. Methods The impact of the extracts on serum glucose levels of diabetic rats was compared with reference drug - glibenclamide-treated diabetic rats. Streptozotocin injection was used to induce diabetes in fasted rats. Various biochemical, physiological and histopathological parameters in diabetic rats were observed for assessing the antidiabetic activity...
April 29, 2017: Journal of Complementary & Integrative Medicine
https://www.readbyqxmd.com/read/28730944/integrating-bioassays-and-analytical-chemistry-as-an-improved-approach-to-support-safety-assessment-of-food-contact-materials
#5
Julien Veyrand, Maricel Marin-Kuan, Claudine Bezencon, Nancy Frank, Violaine Guérin, Sander Koster, Hélia Latado, Julie Mollergues, Amaury Patin, Dominique Piguet, Patrick Serrant, Jesus Varela, Benoît Schilter
Food contact materials (FCM) contain chemicals which can migrate into food and result in human exposure. Although it is mandatory to ensure that migration does not endanger human health, there is still no consensus on how to pragmatically assess the safety of FCM since traditional approaches would require extensive toxicological and analytical testing which are expensive and time consuming. Recently, the combination of bioassays, analytical chemistry and risk assessment has been promoted as a new paradigm to identify toxicologically relevant molecules and address safety issues...
July 21, 2017: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/28730706/chromatographic-profiles-of-tryptophan-and-kynurenine-enantiomers-derivatized-with-s-4-3-isothiocyanatopyrrolidin-1-yl-7-n-n-dimethylaminosulfonyl-2-1-3-benzoxadiazole-using-lc-ms-ms-on-a-triazole-bonded-column
#6
Hideaki Iizuka, Takahiro Harashima, Shuhei Takahashi, Ryosuke Kuwabara, Yoko Naito, Tatsuya Sakamoto, Mayu Onozato, Hideaki Ichiba, Takeshi Fukushima
d- and l-Tryptophan (Trp) and d- and l-kynurenine (KYN) were derivatized with a chiral reagent, (S)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole (DBD-PyNCS), and were separated enantiomerically by high-performance liquid chromatography (HPLC) equipped with a triazole-bonded column (Cosmosil HILIC) using tandem mass spectrometric (MS/MS) detection. Effects of column temperature, salt (HCO2 NH4 ) concentration, and pH of the mobile phase in the enantiomeric separation, followed by MS detection of (S)-DBD-PyNCS-d,l-Trp and -d,l-KYN, were investigated...
July 21, 2017: Chirality
https://www.readbyqxmd.com/read/28730564/a-new-approach-to-assess-occupational-exposure-to-airborne-fungal-contamination-and-mycotoxins-of-forklift-drivers-in-waste-sorting-facilities
#7
Carla Viegas, Tiago Faria, Ana Cebola de Oliveira, Liliana Aranha Caetano, Elisabete Carolino, Anita Quintal-Gomes, Magdalena Twarużek, Robert Kosicki, Ewelina Soszczyńska, Susana Viegas
The waste management industry is an important employer, and exposure of waste-handling workers to microorganisms is considered an occupational health problem. Besides fungal contamination, it is important to consider the co-occurrence of mycotoxins in this setting. Forklifts with closed cabinet and air conditioner are commonly used in waste industry to transport waste and other products within the facilities, possibly increasing the risk of exposure under certain conditions. The aim of this study was to assess the fungal contamination and mycotoxin levels in filters from the air conditioning system of forklift cabinets, as an indicator to assess occupational exposure of the drivers working in a waste sorting facility...
July 20, 2017: Mycotoxin Research
https://www.readbyqxmd.com/read/28730494/deconstructing-the-metabolic-networks-of-oncogenic-signaling-using-targeted-liquid-chromatography-tandem-mass-spectrometry-lc-ms-ms
#8
George Poulogiannis
Metabolic reprogramming is recognized as an emerging hallmark of oncogenic signaling and cancer development. Hence the need to identify novel quantitative analytical platforms for studying metabolism in vitro and in vivo has dramatically increased. Here, we describe the experimental workflow for a targeted liquid chromatography-tandem mass spectrometry (LC-MS/MS) approach involving positive/negative ion switching to analyze >250 metabolites of central carbon metabolism, nucleotides, and amino acids.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730487/a-methodology-for-comprehensive-analysis-of-toll-like-receptor-signaling-in-macrophages
#9
Marijke Koppenol-Raab, Aleksandra Nita-Lazar
A combination of high-throughput, multiplexed, quantitative methods with computational modeling and statistical approaches is required to obtain system-level understanding of biological function. Mass spectrometry (MS)-based proteomics has emerged as a preferred tool for the analysis of changes in protein abundance and their post-translational modification (PTM) levels at a global scale, comparable with genomic experiments and generating data suitable for use in mathematical modeling of signaling pathways. Here we describe a set of parallel bottom-up proteomic approaches to detect and quantify the global protein changes in total intracellular proteins, their phosphorylation, and the proteins released by active and passive secretion or shedding mechanisms (referred to as the secretome as reviewed in Makridakis and Vlahou, J Proteome 73:2291-2305, 2010) in response to the stimulation of Toll-like receptors (TLRs) with specific ligands in cultured macrophages...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730481/label-free-phosphoproteomic-approach-for-kinase-signaling-analysis
#10
Edmund Wilkes, Pedro R Cutillas
Phosphoproteomics is a powerful platform for the unbiased profiling of kinase-driven signaling pathways. Quantitation of phosphorylation can be performed by means of either labeling or label-free mass spectrometry (MS) methods. Because of their simplicity and universality, label-free methodology is gaining acceptance and popularity in molecular biology research. Analytical workflows for label-free quantification of phosphorylation, however, need to overcome several hurdles for the technique to be accurate and precise...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730476/proteomic-profiling-of-protein-kinase-inhibitor-targets-by-mass-spectrometry
#11
Martin Golkowski, Dustin J Maly, Shao-En Ong
Identifying cellular targets of bioactive small molecules from large-scale screening campaigns can be a significant bottleneck in developing novel therapeutics. Our rapid small-molecule target profiling protocol combines affinity enrichment and SILAC for proteomic identification of small molecule-protein interactions. Selective interactions are easily discernable from nonspecific protein binding by quantitative ratios. Using kinase inhibitors as an example, we provide an optimized protocol featuring on-bead protein digestion and single nano-flow liquid chromatographic-mass spectrometric (LC-MS) analyses, consequently increasing analytical throughput and sensitivity over gel-based sample preparation methods for rapid profiling of kinase inhibitor targets...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28730313/derivatization-free-lc-ms-ms-method-for-estrogen-quantification-in-mouse-brain-highlights-a-local-metabolic-regulation-after-oral-versus-subcutaneous-administration
#12
Ecaterina Lozan, Svitlana Shinkaruk, Shaam Alice Al Abed, Valérie Lamothe, Mylène Potier, Aline Marighetto, Jean-Marie Schmitter, Catherine Bennetau-Pelissero, Corinne Buré
17β-Estradiol (17β-E2) is a steroid with pleiotropic actions. In addition to being a sexual hormone, it is also produced in the brain where it modulates the reproductive axis. It has been shown that 17β-E2 also acts on synaptic plasticity and plays a role in neurological pathways and in neurodegenerative diseases. Assaying this steroid in the brain is thus interesting to improve our knowledge of 17β-E2 effects in the brain. However, 17β-E2 concentration in the central nervous system has been reported to be of a few nanograms per gram wet weight (nanomolar range concentration); therefore, its quantification requires both an efficient extraction process and a sensitive detection method...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730312/hydrophilic-interaction-liquid-chromatography-tandem-mass-spectrometry-for-quantitation-of-paralytic-shellfish-toxins-validation-and-application-to-reference-materials
#13
Krista M Thomas, Daniel G Beach, Kelley L Reeves, Ryan S Gibbs, Elliott S Kerrin, Pearse McCarron, Michael A Quilliam
Paralytic shellfish toxins (PSTs) are potent neurotoxins produced by marine dinoflagellates that are responsible for paralytic shellfish poisoning (PSP) in humans. This work highlights our ongoing efforts to develop quantitative methods for PSTs using hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS). Compared with the commonly used method of liquid chromatography with post-column oxidation and fluorescence detection (LC-ox-FLD), HILIC-MS/MS has the potential of being more robust, sensitive and straightforward to operate, and provides unequivocal confirmation of toxin identity...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28730304/novel-ribonuclease-activity-of-cusativin-from-cucumis-sativus-for-mapping-nucleoside-modifications-in-rna
#14
Balasubrahmanyam Addepalli, Sarah Venus, Priti Thakur, Patrick A Limbach
A recombinant ribonuclease, cusativin, was characterized for its cytidine-specific cleavage ability of RNA to map chemical modifications. Following purification of native cusativin protein as described before (Rojo et al. Planta 194:328, 17), partial amino acid sequencing was carried out to identify the corresponding protein coding gene in cucumber genome. Cloning and heterologous expression of the identified gene in Escherichia coli resulted in successful production of active protein as a C-terminal His-tag fusion protein...
July 20, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28729959/shot-gun-proteome-and-transcriptome-mapping-of-the-jujube-floral-organ-and-identification-of-a-pollen-specific-s-locus-f-box-gene
#15
Ruihong Chen, Guoliang Chen, Jian Huang
The flower is a plant reproductive organ that forms part of the fruit produced as the flowering season ends. While the number and identity of proteins expressed in a jujube (Ziziphus jujuba Mill.) flower is currently unknown, integrative proteomic and transcriptomic analyses provide a systematic strategy of characterizing the floral biology of plants. We conducted a shotgun proteomic analysis on jujube flowers by using a filter-aided sample preparation tryptic digestion, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
2017: PeerJ
https://www.readbyqxmd.com/read/28728492/the-bioactivity-of-hedysarum-coronarium-extracts-on-skin-enzymes-and-cells-correlates-with-phenolic-content
#16
Bruno Burlando, Giulia Pastorino, Annalisa Salis, Gianluca Damonte, Marco Clericuzio, Laura Cornara
CONTEXT: The search for bioactive compounds from botanical sources is attracting much interest. However, differences in chemical composition may occur within the same species depending on different geographical origins. OBJECTIVES: We evaluated the properties on skin enzymes and cells of extracts from sulla legume crop Hedysarum coronarium L. (Fabaceae), collected at two Italian sites near Pisa and Ventimiglia, for possible dermatological and cosmetic applications...
December 2017: Pharmaceutical Biology
https://www.readbyqxmd.com/read/28728340/screening-of-differential-proteins-of-placenta-tissues-in-patients-with-pre-eclampsia-by-itraq-proteomics-techniques
#17
Wei-Hong Qi, Ming-Yan Zheng, Chao Li, Lin Xu, Jin-E Xu
BACKGROUND: Isotopically labeled relative and absolute quantification (iTRAQ) were applied together with liquid chromatogram-tandem mass spectrometry (LC-MS/MS) technique to screen and differentiate normal tissues and differential proteins of placenta tissue in the pre-eclampsia group, and to seek for a pre-eclampsia biomarker. METHODS: Thirty patients with severe pre-eclampsia (pre-eclampsia group) and 30 patients with normal full-term pregnancy (control group), who were admitted by the Obstetrical Department of The Affiliated Hospital of Qingdao University from December 2014 to June 2015, were collected for this study...
October 2017: Minerva Medica
https://www.readbyqxmd.com/read/28726498/antibacterial-activity-of-extract-and-fractions-from-branches-of-protium-spruceanum-and-cytotoxicity-on-fibroblasts
#18
Tatiane Roquete Amparo, Ivanildes Vasconcelos Rodrigues, Janaína Brandão Seibert, Rafaella Hilda Zaniti Souza, Amanda Ribeiro de Oliveira, Vivette Appolinário Rodrigues Cabral, Paula Melo de Abreu Vieira, Geraldo Célio Brandão, Adriana Akemi Okuma, Sidney Augusto Vieira Filho, Luiz Fernando Medeiros Teixeira, Gustavo Henrique Bianco de Souza
The crude ethanol extract (CEE) and fractions from branches of Protium spruceanum were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3-80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against Staphylococcus saprophyticus. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC50)...
July 20, 2017: Natural Product Research
https://www.readbyqxmd.com/read/28726485/annals-express-20%C3%AE-and-20%C3%AE-dihydrocortisone-may-interfere-in-lc-ms-ms-determination-of-cortisol-in-saliva-and-urine
#19
Marlen Israelsson, Ralph Brattsand, Goran Brattsand
BACKGROUND: LC-MS/MS methods offer high selectivity in cortisol determinations. However, endogenous steroid metabolites may still interfere and compromise the results, for example in the diagnosis of Cushing´s syndrome. Erroneously elevated cortisol may, in particular, be misleading at the low concentrations found in salivary samples obtained at late night and after dexamethasone suppression. METHODS: Interferences in our LC-MS/MS method used for determination of cortisol in saliva and urine were identified by comparing their retention times and mass spectra with those of pure candidate substances...
January 1, 2017: Annals of Clinical Biochemistry
https://www.readbyqxmd.com/read/28726036/proteolytic-effects-of-gingipains-on-trefoil-factor-family-peptides
#20
Ponlatham Chaiyarit, Janthima Jaresitthikunchai, Narumon Phaonakrop, Sittiruk Roytrakul, Barbara Potempa, Jan Potempa
OBJECTIVES: The present study was aimed to determine whether trefoil factor family (TFF) peptides which were generally considered to be resistant to proteolysis could be digested by gingipains, a major proteinases produced by Porphyromonas gingivalis. MATERIALS AND METHODS: Recombinant human TFF1, TFF2, and TFF3 peptides were used as substrates. Gingipains including arginine gingipain (RgpB) and lysine gingipain (Kgp) were used as enzymes. Trypsin was used as a control protease...
July 19, 2017: Clinical Oral Investigations
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