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Brandon Presley, Bob Bianchi, John Coleman, Fran Diamond, Gerry McNally
Clandestine chemists have demonstrated an ability to convert commercially available pseudoephedrine formulations to methamphetamine. Some of these formulations have properties that manufacturers claim limit or block the extraction of pseudoephedrine and its direct conversion to methamphetamine. In this study, 3 commercially available pseudoephedrine formulations were evaluated for ease of extraction and conversion to methamphetamine using a common chemistry technique called the one-pot method that is frequently employed by clandestine chemists...
April 16, 2018: Journal of Pharmaceutical and Biomedical Analysis
S Senthil Kumar, P Muthuselvam, V Pugalenthi, N Subramanian, K M Ramkumar, T Suresh, T Suzuki, P Rajaguru
Toxicoproteomic analysis of steel industry ambient particulate matter (PM) that contain high concentrations of PAHs and metals was done by treating human lung cancer cell-line, A549 and the cell lysates were analysed using quantitative label-free nano LC-MS/MS. A total of 18,562 peptides representing 1576 proteins were identified and quantified, with 196 proteins had significantly altered expression in the treated cells. Enrichment analyses revealed that proteins associated to redox homeostsis, metabolism, and cellular energy generation were inhibited while, proteins related to DNA damage and repair and other stresses were over expressed...
April 20, 2018: Environmental Pollution
P Johnsi Rani, Chiguru Vishnuvardhan, Rakesh D Nimbalkar, Prabha Garg, N Satheeshkumar
The focus of the present study is on in vitro and in vivo metabolite identification of ambrisentan (AMBR) a selective endothelin type - A (ETA) receptor antagonist using quadruple time-of-flight mass spectrometry (QTOF/MS). in vitro metabolism study was conducted by incubating AMBR in rat liver microsomes (RLM), rat and human liver S9 fractions. In vivo study was carried out through the collection of urine, faeces and plasma samples at various time points after oral administration of AMBR in suspension form at a dose of 25 mg/kg to six male Sprague - Dawley (SD) rats...
April 12, 2018: Journal of Pharmaceutical and Biomedical Analysis
Piotr J Rudzki, Elżbieta Gniazdowska, Katarzyna Buś-Kwaśnik
Liquid chromatography coupled to mass spectrometry (LC-MS) is a powerful tool for studying pharmacokinetics and toxicokinetics. Reliable bioanalysis requires the characterization of the matrix effect, i.e. influence of the endogenous or exogenous compounds on the analyte signal intensity. We have compared two methods for the quantitation of matrix effect. The CVs(%) of internal standard normalized matrix factors recommended by the European Medicines Agency were evaluated against internal standard normalized relative matrix effects derived from Matuszewski et al...
April 6, 2018: Journal of Pharmaceutical and Biomedical Analysis
Francisco Zarate-Perez, Jesús B Velázquez-Fernández, Gareth K Jennings, Lisa S Shock, Charles E Lyons, John C Hackett
Cytochrome P450 19 (CYP19, aromatase) catalyzes the conversion of androgens to estrogens in a sequence of three reactions that each depend on NADPH and O2 . Aromatase is a phylogenetically-ancient enzyme and its breadth of expression in other species has highlighted distinct physiological functions. In songbirds, estrogen production is required for programming the neural circuits controlling song and in the determination of sex in fish and reptiles. This work describes the expression, purification, and biophysical characterization of Aptenodytes forsteri (Emperor penguin, af) aromatase...
April 7, 2018: Journal of Inorganic Biochemistry
Zenglei Hu, Han Gu, Jiao Hu, Shunlin Hu, Xiaoquan Wang, Xiaowen Liu, Xinan Jiao, Xiufan Liu
Pathogenesis of genotype VII Newcastle disease virus (NDV) is characterized with remarkable immunopathology in the spleen in chickens. However, the mechanism for this unique pathological phenotype is not fully understood. Previous transcriptomics data showed that genotype VII NDV JS5/05 caused a greater downregulation of extracellular matrix (ECM) genes than genotype IV virus Herts/33 in the spleen. In this study, the role of ECM in pathology of genotype VII NDV was investigated using quantitative proteomics...
April 20, 2018: Journal of Proteomics
Stewart F Graham, Xiaobei Pan, Ali Yilmaz, Shirin Macias, Andrew Robinson, David Mann, Brian D Green
Huntington's disease (HD) is a devastating, progressive neurodegenerative disease with a distinct phenotype characterized by chorea and dystonia, incoordination, cognitive decline and behavioral difficulties. The precise mechanisms of HD progression are poorly understood; however, it is known that there is an expansion of the trinucleotide cytosine-guanosine-guanine (CAG) repeat in the Huntingtin gene. Herein DI/LC-MS/MS was used to accurately identify and quantify 185 metabolites in post mortem frontal lobe and striatum from HD patients and healthy control cases...
April 20, 2018: Biochimica et Biophysica Acta
Kumiko Nishimura, Masafumi Takeda, Jun K Yamashita, Ichiro Shiojima, Nagaoki Toyoda
AIMS: Type 3 iodothyronine deiodinase (D3), which converts thyroxine (T4 ) and 3,5,3'-triiodothyronine (T3 ) to 3,3',5'-triiodothyronine (rT3 ) and 3,3'-diiodothyronine (T2 ), respectively, inactivates thyroid hormones. We investigated the expression and regulation of D3 in human cardiomyocytes which were differentiated from human induced pluripotent stem cells (hiPSCs). MAIN METHODS: We characterized D3 activity using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis...
April 20, 2018: Life Sciences
Gallant K L Chan, Winnie W H Hu, Zoey X Zheng, M Huang, Yan X Y Lin, Caroline Y Wang, Amy G W Gong, X Y Yang, Karl W K Tsim, Tina T X Dong
Dementia is a persistent disorder of the mental processes and is strongly related to depression. However, the performance of current antidepression medicine is far from satisfactory. Herbal extract provides an excellent source to identify compounds for possible drug development against depression. Here, HerboChips were employed to search herbal compounds that could bind nerve growth factor (NGF). By screening over 500 types of herbal extracts, the water extract of Ginkgo Folium, the leaf of Ginkgo biloba , showed a strong binding to NGF...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Lenin D Sarabia, Berin A Boughton, Thusitha Rupasinghe, Allison M L van de Meene, Damien L Callahan, Camilla B Hill, Ute Roessner
Introduction: Mass spectrometry imaging (MSI) is a technology that enables the visualization of the spatial distribution of hundreds to thousands of metabolites in the same tissue section simultaneously. Roots are below-ground plant organs that anchor plants to the soil, take up water and nutrients, and sense and respond to external stresses. Physiological responses to salinity are multifaceted and have predominantly been studied using whole plant tissues that cannot resolve plant salinity responses spatially...
2018: Metabolomics: Official Journal of the Metabolomic Society
Chin-Hsiung Wang, Hung Su, Jo-Han Chou, Min-Zong Huang, Hsing-Jung Lin, Jentaie Shiea
Labor- and time-intensive sample preparation and liquid chromatography mass spectrometry (LC-MS) analysis are required for traditional pharmacokinetics (PK) studies. In order to simplify and accelerate the analytical process of the PK study, solid phase microextraction (SPME) combined with thermal desorption-electrospray ionization/mass spectrometry (TD-ESI/MS) was developed for rapid characterization of trace drug in biological fluids. Methylphenidate in plasma was extracted and concentrated by direct immersion SPME using fused-silica fibers coated with polydimethylsiloxane...
August 27, 2018: Analytica Chimica Acta
Ling Wang, Xin-Yi Lan, Jun Ji, Chun-Feng Zhang, Fei Li, Chong-Zhi Wang, Chun-Su Yuan
Rheumatoid arthritis (RA) is one of the most prevalent chronic inflammatory and angiogenic diseases. The aim of this study was to evaluate the anti-inflammatory and anti-angiogenic activities in vitro of eight diterpenoids isolated from Daphne genkwa. LC-MS was used to identify diterpenes isolated from D. genkwa. The anti-inflammatory and anti-angiogenic activities of eight diterpenoids were evaluated on LPS-induced macrophage RAW264.7 cells and TNF-α-stimulated human umbilical vein endothelial cells (HUVECs) using hierarchical cluster analysis (HCA) and principal component analysis (PCA)...
April 21, 2018: Journal of Natural Medicines
Ching-Yu Lin, Jau-Jiuan Sheu, I-Shih Tsai, Sen-Te Wang, Li-Yu Yang, I-Uen Hsu, Hui-Wen Chang, Horng-Mo Lee, Shu-Huei Kao, Ching-Kuo Lee, Chien-Ho Chen, Yung-Feng Lin
OBJECTIVE: Advanced glycation end products (AGEs) are involved in the pathogenesis of Alzheimer's disease (AD). Specific AGEs and related autoantibodies may be early AD markers. Apolipoprotein A1 (ApoA1) and its post-translational modifications (PTMs) are associated with neurodegeneration and thus selected to test the hypothesis. METHODS: Serum samples from totally 64 AD or health control (HC) Taiwanese were analyzed. ApoA1 was isolated from the serum and examined through LC-MS/MS and PTM analyses...
April 19, 2018: Clinical Biochemistry
Xinying Hong, Arun Babu Kumar, C Ronald Scott, Michael H Gelb
All States screen for biotinidase deficiency and galactosemia, and X-linked adrenoleukodystrophy (X-ALD) has recently been added to the Recommended Uniform Screening Panel (RUSP).We sought to consolidate these tests by combining them into a single multiplex tandem mass spectrometry assay as well as to improve the current protocol for newborn screening of galactosemia.A 3 mm punch of a dried blood spot (DBS) was extracted with organic solvent for analysis of the C26:0-lysophosphatidylcholine biomarker for X-ALD...
March 29, 2018: Molecular Genetics and Metabolism
Dayana M Dos Santos, Lucas Buruaem, Renato M Gonçalves, Mike Williams, Denis M S Abessa, Rai Kookana, Mary Rosa R de Marchi
Submarine sewage outfalls (SSOs) are considered the main input source of contaminants of emerging concern continuously released in coastal areas, with the potential to cause adverse effects for aquatic organisms. This work presents the investigation of nine endocrine disrupting chemicals (EDCs) and 26 pharmaceutically active chemicals (PhACs) in marine sediments within the vicinities of 7 SSOs along the São Paulo State Coast (Brazil). Method optimization for the multi-residue determination by GC-MS/MS and LC-MS/MS using QuEChERS extraction/clean-up are discussed...
April 2018: Marine Pollution Bulletin
Buxin Chen, Chen Li, Yeda Wang, Yuanan Lu, Fang Wang, Xueqin Liu
Spring viremia of carp virus (SVCV) is a fierce pathogen causing high mortality in the common carp. The glycoprotein (G protein) of SVCV is a pivotal component of the viral structure, located in the surface of the virion, and plays a key role in viral endocytosis. In this study, tandem affinity purification (TAP) followed by mass spectrometry analysis (LC-MS/MS) was carried out to search for novel host molecules that interact with SVCV G protein and a 14-3-3β/α-A protein was identified. The level of 14-3-3β/α-A mRNA expression was dramatically down regulated by SVCV infection...
April 19, 2018: Fish & Shellfish Immunology
Lei Chen, Jinsong Li, Xuan Ke, Cailing Sun, Xiaoxian Huang, Pan Jiang, Feng Feng, Jie Zhang, Wenyuan Liu
ETHNOPHARMACOLOGICAL RELEVANCE: Periploca forrestii Schltr. is a popular folk medicine in china, commonly prescribed for the treatment of rheumatoid arthritis and wounds. The present research aimed to evaluate the effects of HLG on wound healing and reveal the potential active constituents. MATERIALS AND METHODS: The wound healing activity was assessed by proliferation of fibroblast, migration and collagen production using L929 cells. A reliable HPLC-Q-TOF-MS/MS method was constructed for the systematic identification and characterization of main components in HLG...
April 19, 2018: Journal of Ethnopharmacology
Wen Ma, Pierre Waffo-Téguo, Michäel Jourdes, Hua Li, Pierre-Louis Teissedre
Flavan-3-ols are units incorporating condensed tannin, which are widely present in grape and wine. They play a considerable role in wine sensory perception such as astringency, bitterness and mouth-feel. In grape and wine, the flavan-3-ols reported to date are (epi)catechin, (epi)gallocatechin, (epi)gallocatechin gallate and (epi)catechin glycoside. This study now shows the presence of a new flavan-3-ol epicatechin vanillate in grape seed and red wine. A putative unknown flavan-3-ol derived from grape seed was targeted by LC-HRMS/MS...
September 1, 2018: Food Chemistry
Forough Jahandideh, Ping Liu, Jianping Wu
Egg proteins are a good source of bioactive peptides. Previous work from our research group has demonstrated the potential of egg white hydrolysate (EWH) for enhancing adipogenic differentiation and insulin signalling in 3T3-F442A pre-adipocytes. EWH was prepared by a combination of thermolysin and pepsin. Here, in this study, we aimed to identify the responsible peptide(s) in EWH. EWH was fractionated stepwise by ultrafiltration, C18 Sep-Pack cartridge, cation-exchange chromatography, and reverse-phase chromatography...
September 1, 2018: Food Chemistry
Ramon Cueto, Lixiao Zhang, Hui Min Shan, Xiao Huang, Xinyuan Li, Ya-Feng Li, Jahaira Lopez, William Y Yang, Muriel Lavallee, Catherine Yu, Yong Ji, Xiaofeng Yang, Hong Wang
Hyperhomocysteinemia (HHcy) is an independent risk factor for cardiovascular disease (CVD) which has been implicated in matochondrial (Mt) function impairment. In this study, we characterized Hcy metabolism in mouse tissues by using LC-ESI-MS/MS analysis, established tissue expression profiles for 84 nuclear-encoded Mt electron transport chain complex (nMt-ETC-Com) genes in 20 human and 19 mouse tissues by database mining, and modeled the effect of HHcy on Mt-ETC function. Hcy levels were high in mouse kidney/lung/spleen/liver (24-14 nmol/g tissue) but low in brain/heart (~5 nmol/g)...
April 4, 2018: Redox Biology
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