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https://www.readbyqxmd.com/read/28945015/membrane-fusion-mediated-intracellular-delivery-of-lipid-bilayer-coated-mesoporous-silica-nanoparticles
#1
Jian Yang, Jing Tu, Gerda E M Lamers, René C L Olsthoorn, Alexander Kros
Protein delivery into the cytosol of cells is a challenging topic in the field of nanomedicine, because cellular uptake and endosomal escape are typically inefficient, hampering clinical applications. In this contribution cuboidal mesoporous silica nanoparticles (MSNs) containing disk-shaped cavities with a large pore diameter (10 nm) are studied as a protein delivery vehicle using cytochrome-c (cytC) as a model membrane-impermeable protein. To ensure colloidal stability, the MSNs are coated with a fusogenic lipid bilayer (LB) and cellular uptake is induced by a complementary pair of coiled-coil (CC) lipopeptides...
September 25, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28945005/differential-expression-of-myometrial-ap-1-proteins-during-gestation-and-labour
#2
Lubna Nadeem, Tali Farine, Anna Dorogin, Elzbieta Matysiak-Zablocki, Oksana Shynlova, Stephen Lye
Preterm labour (PTL) is a leading cause of perinatal mortality and postnatal morbidity. Contractions of the uterine muscle (myometrium) that determine the onset of labour depend on the expression of contraction-associated proteins (CAPs, i.e. connexin43) regulated by dimeric AP-1 transcription factors. Here, we examined subcellular (by immunoblotting) and tissue expression (by immunohistochemistry) of myometrial AP-1 proteins (cJUN, JUNB, JUND, cFOS, FOSB, FRA1, FRA2) throughout gestation and TL in different species (mouse, rat and human)...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28945004/tlr3-contributes-to-persistent-autophagy-and-heart-failure-in-mice-after-myocardial-infarction
#3
Ting Gao, Shao-Ping Zhang, Jian-Fei Wang, Li Liu, Yin Wang, Zhi-Yong Cao, Qi-Kuan Hu, Wen-Jun Yuan, Li Lin
Toll-like receptors (TLRs) are essential immunoreceptors involved in host defence against invading microbes. Recent studies indicate that certain TLRs activate immunological autophagy to eliminate microbes. It remains unknown whether TLRs regulate autophagy to play a role in the heart. This study examined this question. The activation of TLR3 in cultured cardiomyocytes was observed to increase protein levels of autophagic components, including LC3-II, a specific marker for autophagy induction, and p62/SQSTM1, an autophagy receptor normally degraded in the final step of autophagy...
September 25, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28944959/antimicrobial-activity-of-lysozyme-isoforms-key-molecular-features
#4
Melanie Derde, Véronique Vié, Astrid Walrant, Sandrine Sagan, Valérie Lechevalier, Catherine Guérin-Dubiard, Stéphane Pezennec, Marie-Françoise Cochet, Gilles Paboeuf, Maryvonne Pasco, Florence Baron, Michel Gautier, Sophie Jan, Françoise Nau
Increasing bacterial resistance towards antibiotics has stimulated research for novel antimicrobials. Proteins acting on bacterial membranes could be a solution. Lysozyme has been proven active against E. coli by disruption of both outer and cytoplasmic membranes, with dry-heating increasing lysozyme activity. Dry-heated lysozyme (DH-L) is a mixture of isoforms (isoaspartyl, native-like and succinimide lysozymes), giving rise to two questions: what effects does each form have, and which physicochemical properties are critical as regards the antibacterial activity? These issues were investigated by fractionating DH-L, analyzing structural properties of each fraction, and testing each fraction in vivo on bacteria and in vitro on membrane models...
September 25, 2017: Biopolymers
https://www.readbyqxmd.com/read/28944928/hydrogen-inhibits-isoproterenol%C3%A2-induced-autophagy-in-cardiomyocytes-in%C3%A2-vitro-and-in%C3%A2-vivo
#5
Yaxing Zhang, Zhiyuan Long, Jingting Xu, Sihua Tan, Nan Zhang, Anfei Li, Ling Wang, Tinghuai Wang
A previous study from our group has demonstrated that hydrogen administration can attenuate cardiovascular hypertrophy in vivo by targeting reactive oxygen species‑dependent mitogen‑activated protein kinase signaling. The aim of the present study is to determine the effect of hydrogen on cardiomyocyte autophagy during β‑adrenoceptor activation in vivo and in vitro. We prepared hydrogen‑rich medium, and the concentration of hydrogen was measured by using the MB‑Pt reagent method. For the in vitro study, H9c2 cardiomyocytes were stimulated with isoproterenol (ISO; 10 µM) for 5, 15 and 30 min, and then the protein expression levels of the autophagy marker microtubule‑associated protein 1 light chain 3β II (LC3B II) were examined by western blotting...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944925/autophagy-is-activated-to-protect-renal-tubular-epithelial-cells-against-iodinated-contrast-media%C3%A2-induced-cytotoxicity
#6
Na Liu, Rong Lei, Mi-Mi Tang, Wei Cheng, Min Luo, Qian Xu, Shao-Bin Duan
With the steady increase in the use of various contrast‑related technologies in recent years, contrast‑induced nephropathy has received significant attention from clinicians and researchers. The present study aimed to determine the potential role of autophagy in iodinated contrast media (CM)‑induced cell injury and apoptosis in human proximal renal tubular epithelial HK‑2 cells. The present study used the iodinated CM iohexol (200 mg iodine/ml) to treat HK‑2 cells for 6 h, in order to establish a damaged cell model...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944915/neuroprotective-effect-of-total-flavonoids-from-ilex-pubescens-against-focal-cerebral-ischemia-reperfusion-injury-in-rats
#7
Xiaoyan Fang, Yujie Li, Jingyi Qiao, Ying Guo, Mingsan Miao
Ilex pubescens is commonly used in traditional Chinese medicine to treat cardiovascular and cerebrovascular diseases, such as coronary artery disease and stroke. However, the underlying mechanisms remain to be fully elucidated. The aim of the present study was to investigate the effects of Ilex pubescens total flavonoids (IPTF) on neuroprotection and the potential mechanisms in a rat model of focal cerebral ischemia/reperfusion (I/R) injury. Rats were pretreated with intragastric administration of IPTF at doses of 200 and 100 mg/kg for 5 days; middle cerebral artery occlusion surgery was then performed to induce cerebral I/R injury...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944913/oleanolic-acid-alleviates-diabetic-rat-carotid-artery-injury-through-the-inhibition-of-nlrp3-inflammasome-signaling-pathways
#8
Qian An, Qian Hu, Bing Wang, Wenjun Cui, Fei Wu, Yu Ding
The overexpression of inflammasome components is correlated with diabetes‑associated complications. Oleanolic acid is a triterpenoid compound which is important in arterial injury. The present study evaluated whether oleanolic acid improved diabetic rat carotid artery injury through the inhibition of nucleotide‑binding domain, leucine‑rich‑containing family, pyrin domain‑containing‑3 (NLRP3) inflammasomes signaling pathways. A diabetic rat model was induced using streptozotocin (60 mg/kg) and underwent carotid artery injury...
September 25, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944912/dhhp%C3%A2-6-attenuates-cerebral-ischemia%C3%A2-reperfusion-injury-in-rats-through-the-inhibition-of-apoptosis
#9
Yingshi Ji, Xin Yan, Yang Hu, Huan Xue, Jianfeng Sun, Huaqiu Chen, Jiayu Zhang, Liping Wang, Baigong Xue, Li Sun
As a novel reactive oxygen species (ROS) scavenger, deuterohemin His peptide‑6 (DhHP‑6) has been demonstrated to prolong the lifespan of Caenorhabditis elegans and has also exhibited protective effects in myocardial ischemia‑reperfusion injury. Whether similar effects occur during cerebral ischemia‑reperfusion (CIR) injury remains to be elucidated. The present study evaluated the function of DhHP‑6 and its underlying mechanisms in a middle cerebral artery occlusion (MCAO) model in rats. The focal transient MCAO model was implemented using the Longa method of ischemia for 2 h followed by reperfusion for 22 h in male Wistar rats...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944903/gpvi%C3%A2-fc%C3%A2-peg-improves-cerebral-infarct-volume-and-cerebral-thrombosis-in-mouse-model-with-cerebral-thrombosis
#10
Yimae Wufuer, Xuefeng Shan, Magaoweiya Sailike, Kamile Adilaimu, Songfeng Ma, Huguo Wang
Cerebral thrombosis is one of the most common causes of cerebral infarction, and anticoagulation therapy is a routine treatment in patients with hemorrhagic cerebral venous thrombosis. The hemostatic function of platelets is important for the anticoagulation therapy of thrombosis. Glycoprotein VI (GPVI) is reported as the major signaling receptor for collagen and is exclusively expressed on platelets and megakaryocytes, initiating platelet recruitment at sites of vascular injury and demonstrating numerous beneficial effects for patients with cerebral thrombosis...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944889/beneficial-effects-of-extracts-from-lucilia%C3%A2-sericata-maggots-on-burn-wounds-in-rats
#11
Haixu Bian, Qiaoli Yang, Tao Ma, Wei Li, Jialin Duan, Guo Wei, Xiaoxiao Wu, Fei Mu, Rui Lin, Aidong Wen, Miaomiao Xi
Lucilia sericata maggots have beneficial properties; however, their protective effects on burn wounds have yet to be fully elucidated. In the present study, a deep second‑degree burn rat model was used to investigate the burn wound healing properties of aqueous extract of maggots (MAE). The anti‑inflammatory, antioxidative and antibacterial activities were examined. In addition, the protein expression levels of Akt, vascular endothelial growth factor A (VEGFA) and nuclear factor‑κB (NF‑κB) were detected by western blotting...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944886/sulforaphane-induces-p53%C3%A2-deficient-sw480-cell-apoptosis-via-the-ros%C3%A2-mapk-signaling-pathway
#12
Hai Lan, Hongyin Yuan, Congyao Lin
Sulforaphane (SFN) has been revealed to inhibit the growth and induce apoptosis of cancer cells. However, the detailed anticancer effects of SFN on p53‑deficient colon cancer cells has yet to be clearly elucidated. The present study employed p53‑deficient SW480 cells to establish an SFN‑induced in vitro model of apoptosis. The critical events leading to apoptosis were then evaluated in SFN‑treated p53‑deficient SW480 cells, by performing an MTT assay, flow cytometry, western blotting and ELISA. The results demonstrated that SFN at concentrations of 5, 10, 15 and 20 µM induced mitochondria‑associated cell apoptosis, which was further confirmed by disruption of the mitochondrial membrane potential, an increase in the Bax/Bcl‑2 ratio, as well as activation of caspase‑3, ‑7 and ‑9...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944880/edoxaban-improves-venous-thrombosis-via-increasing-hydrogen-sulfide-and-homocysteine-in-rat-model
#13
Wei Song, Hongbo Ci, Guanglei Tian, Yanjun Zhang, Xiaohu Ge
Anticoagulant therapy is prescribed to millions of patients worldwide for the prevention and treatment of venous thrombosis. Evidence has indicated that edoxaban is a potential drug of oral anticoagulant in the acute treatment of venous thromboembolism. Hydrogen sulfide and homocysteine plasma concentration are indicators of cardiovascular and neurovascular disease risk factors that have attracted considerable attention for regulation of vascular health and homeostasis. However, the molecular mechanism of edoxaban‑mediated differences of hydrogen sulfide and homocysteine has not been investigated in the progression of venous thrombosis...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944876/nutrient-deprivation-induces-apoptosis-of-nucleus-pulposus-cells-via-activation-of-the-bnip3-aif-signalling-pathway
#14
Jie Liu, Chao Yuan, Luqiao Pu, Jian Wang
Nutrient deprivation (ND)‑induced nucleus pulposus (NP) cell death serves an important role in intervertebral disc degeneration disease. However, the underlying mechanisms have yet to be thoroughly elucidated. The present study created a cell culture model under ND conditions to investigate the roles of the nutrient‑sensitive protein B‑cell lymphoma 2/adenovirus E1B 19 kDa‑interacting protein (BNIP3) and the mitochondrial pro‑death protein apoptosis‑inducing factor (AIF) in the death pathway of NP cells...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944869/promoting-potential-of-adipose-derived-stem-cells-on-peripheral-nerve-regeneration
#15
Jiayan Guo, Shu Guo, Yuxin Wang, Yanqiu Yu
The ultimate goal of treating peripheral nerve defects is reconstructing continuity of the nerve stumps to regain nerve conduction and functional recovery. Clinically, autologous nerve grafts and Schwann cell (SC) therapy have limitations, such as the need for secondary surgery, sacrifice of another nerve and donor site complication. Adipose derived stem cells (ADSCs) may promise to be ideal alternative cells of SCs. To explore the potential of ADSCs promoting peripheral nerve regeneration, the present study investigated the influences of ADSCs on proliferation and neurotrophic function of SCs using co‑culture model in vitro...
September 21, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944866/early-anticoagulation-therapy-for-severe-burns-complicated-by-inhalation-injury-in-a-rabbit-model
#16
Zhong-Hua Fu, Guang-Hua Guo, Zhen-Fang Xiong, Xincheng Liao, Ming-Zhuo Liu, Jinhua Luo
The aim of the present study was to determine the effects of early anticoagulation treatment on severe burns complicated by inhalation injury in a rabbit model. Under anesthetization, an electrical burns instrument (100˚C) was used to scald the backs of rabbits for 15 sec, which established a 30% III severe burns model. Treatment of the rabbits with early anticoagulation effectively improved the severe burns complicated by inhalation injury‑induced lung injury, reduced PaO2, PaCO2 and SPO2 levels, suppressed the expression of tumor necrosis factor‑α, interleukin (IL)‑1β and IL‑6, and increased the activity of IL‑10...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944856/systemic-overexpression-of-the-11%C3%AE-%C3%A2-hsd1-promotes-endoplasmic-reticulum-stress-in-multiple-tissues-and-the-development-of-metabolic-syndrome-in-mice
#17
Chenxiao Li, Jihan Xia, Wenjuan Zhu, Leilei Xin, Cuiping An, Shulin Yang, Kui Li
Glucocorticoids are associated with lipid metabolism and their abnormal expression has an important function in the development of metabolic syndrome. The 11β‑hydroxysteroid dehydrogenase type 1 (11β‑HSD1) is a metabolic enzyme of glucocorticoids and may be a potential drug target for the treatment of metabolic syndrome. However, the association between the systemic expression of 11β‑HSD1 and metabolic syndrome remains to be elucidated. The present study used a cytomegalovirus promoter to obtain mice that systemically overexpressed the 11β‑HSD1 gene...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944854/effect-of-hippocampal-l%C3%A2-nbp-on-bdnf-and-trkb-expression-and-neurological-function-of-vascular-dementia-rats
#18
Yujing Huang, Zhaohui Li, Guangxian Nan
The pathogenesis of vascular dementia (VD) is associated with neuronal degeneration, apoptosis or necrosis following ischemic brain injury. L‑butylphthalide (L‑NBP), has been demonstrated to exhibit potent anti‑ischemic and anti‑VD effects, however the associated specific mechanism remains to be elucidated. The present study generated a VD rat model, in which the effect of L‑NBP on neurological function and expression levels of brain‑derived neurotrophic factor (BDNF) and tyrosine kinase receptor B (TrkB) were observed...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944852/use-of-immunoproteomics-to-identify-immunogenic-proteins-in-a-rat-model-of-acute-respiratory-distress-syndrome
#19
Zongshu Ji, Haiyan Liu, Linsen Fang, Youxin Yu, Zheng Zhou
Acute respiratory distress syndrome (ARDS) is a common and life‑threatening clinical syndrome, and seeking biomarkers of ARDS has been an area of continuing research. The present study hypothesized that alterations to certain immunogenic substances occur in injured lungs and are able to specifically bind with corresponding proteins in the blood, and that these proteins may be readily detected. To investigate this hypothesis, a rat model of ARDS was established by cecal ligation and puncture surgery, and an immunoproteomics approach, using serum as the primary antibody in a western blot analysis, was used with the aim of identifying immunogenic proteins in the injured lungs...
September 20, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944847/geniposide-promotes-autophagy-to-inhibit-insulin-resistance-in-hepg2-cells-via-p62-nf%C3%A2-%C3%AE%C2%BAb-glut%C3%A2-4
#20
Hongwei Jiang, Yujin Ma, Junqiang Yan, Jie Liu, Liping Li
Insulin resistance (IR) is known to be an important factor, which can lead to the onset of type 2 diabetes. Autophagy is a cellular process, which sequesters senescent or damaged proteins in autophagosomes for recycling of their products. Insulin and intracellular molecules, including mammalian target of rapamycin (mTOR), are well‑known inhibitors of autophagy. In patients with type 2 diabetes, the expression levels of glucose transporter 4 (GLUT‑4) in skeletal muscles are significantly decreased, indicating decreased glucose‑processing ability...
September 18, 2017: Molecular Medicine Reports
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