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https://www.readbyqxmd.com/read/29244820/probiotics-reduce-repeated-water-avoidance-stress-induced-colonic-microinflammation-in-wistar-rats-in-a-sex-specific-manner
#1
Ju Yup Lee, Nayoung Kim, Ryoung Hee Nam, Sung Hwa Sohn, Sun Min Lee, Daeun Choi, Hyuk Yoon, Yong Sung Kim, Hye Seung Lee, Dong Ho Lee
The colonic response to stress is greater in female rats than in male rats. The aim of this study was to evaluate the effect of probiotics in the repeated water avoidance stress (rWAS)-induced colonic microinflammation model of Wistar rats in a sex-specific manner. The three groups (no-stress, WAS, and WAS with probiotics) were exposed to r-WAS for 1 h daily for 10 days, and Lactobacillus farciminis was administered by oral gavage for 10 days to animals in the probiotics group. The visceromotor response (VMR) to colorectal distension (CRD) was assessed using a barostat and noninvasive manometry before and after WAS exposure...
2017: PloS One
https://www.readbyqxmd.com/read/29243027/lactobacillus-rhamnosus-reduces-parasite-load-on-toxocara-canis-experimental-infection-in-mice-but-has-no-effect-on-the-parasite-in-vitro
#2
Débora Liliane Walcher, Luis Augusto Xavier Cruz, Paula de Lima Telmo, Lourdes Helena Rodrigues Martins, Luciana Farias da Costa de Avila, Maria Elisabeth Aires Berne, Carlos James Scaini
Human toxocariasis is a neglected global parasitic zoonosis. The efficacy of drug treatment for this disease has been hindered by the biological complexity of the main etiological agent, the nematode Toxocara canis. Experimental studies have shown the potential of probiotics to promote a reduction in the parasite load of T. canis larvae. This study aimed to evaluate the effect of probiotic Lactobacillus rhamnosus ATCC 7469 on the parasite load of BALB/c mice with acute toxocariasis and evaluate the direct effect of this probiotic on T...
December 14, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29242832/persistence-of-supplemented-bifidobacterium-longum-subsp-infantis-evc001-in-breastfed-infants
#3
Steven A Frese, Andra A Hutton, Lindsey N Contreras, Claire A Shaw, Michelle C Palumbo, Giorgio Casaburi, Gege Xu, Jasmine C C Davis, Carlito B Lebrilla, Bethany M Henrick, Samara L Freeman, Daniela Barile, J Bruce German, David A Mills, Jennifer T Smilowitz, Mark A Underwood
Attempts to alter intestinal dysbiosis via administration of probiotics have consistently shown that colonization with the administered microbes is transient. This study sought to determine whether provision of an initial course of Bifidobacterium longum subsp. infantis (B. infantis) would lead to persistent colonization of the probiotic organism in breastfed infants. Mothers intending to breastfeed were recruited and provided with lactation support. One group of mothers fed B. infantis EVC001 to their infants from day 7 to day 28 of life (n = 34), and the second group did not administer any probiotic (n = 32)...
November 2017: MSphere
https://www.readbyqxmd.com/read/29242550/diversity-of-vaginal-microbiota-increases-by-the-time-of-labor-onset
#4
Ekaterina Avershina, Silje Slangsvold, Melanie Rae Simpson, Ola Storrø, Roar Johnsen, Torbjørn Øien, Knut Rudi
Vaginal microbiota is an important early source of bacterial colonization for newborns. However, only a few small studies have investigated the composition of vaginal microbiota during labor. In this work, we analyzed vaginal swabs collected at 36 weeks gestation and at the onset of labor from 256 women participating in a randomized placebo-controlled study of probiotic supplementation for the prevention of atopic dermatitis in offspring. Although individuals' vaginal microbiota was stable over time, several bacterial families, which are characteristic of mixed community state type (CST) IV, were overrepresented in vaginal swabs sampled at labor...
December 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29241321/the-suitability-of-different-probiotic-strains-for-the-production-of-fruit-whey-beverages
#5
Marek Sady, Dorota Najgebauer-Lejko, Jacek Domagała
BACKGROUND: When designing new probiotic products, one of the most important aspects is the selection of bacterial strains with high survival rates in the matrix of the product concerned. The aim of the present research was to evaluate the potential of selected strains of probiotic bacteria for the production of fruit-whey beverages. METHODS: Orange, apple and blackcurrant whey beverages were produced, and each was inoculated with one of the following probiotic strains: Bifidobacterium lactis HN019TM; Lactobacillus aci- dophilus NCFM®; Lactobacillus paracasei Lpc-37TM; Lactobacillus rhamnosus HN001TM...
October 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29238921/bacillus-probiotic-supplementations-improve-laying-performance-egg-quality-hatching-of-laying-hens-and-sperm-quality-of-roosters
#6
Maria S Mazanko, Ivan F Gorlov, Evgeniya V Prazdnova, Maxim S Makarenko, Alexander V Usatov, Anzhelika B Bren, Vladimir A Chistyakov, Alexey V Tutelyan, Zoya B Komarova, Natalia I Mosolova, Denis N Pilipenko, Olga E Krotova, Aleksandr N Struk, Angela Lin, Michael L Chikindas
The study aims at elucidating the effect of bacilli probiotic preparations on the physiology of laying hens and roosters. Probiotic formulations were prepared as soybean products fermented by Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895. In this study, groups of male and female chickens were used. These groups received a probiotic preparation based on either B. subtilis KATMIRA1933 or B. amyloliquefaciens B-1895, or of a mixture of strains, from the first day to the age of 39 weeks. These preparations positively affected egg production, quality of sperm production, and quality and hatchery of eggs...
December 13, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29238459/isolation-and-characterization-of-probiotics-from-dairies
#7
Babak Haghshenas, Yousef Nami, Ali Almasi, Norhafizah Abdullah, Dayang Radiah, Rozita Rosli, Abolfazl Barzegari, Ahmad Yari Khosroushahi
Background and Objectives: Probiotics are live microorganisms, which show beneficial health effects on hosts once consumed in sufficient amounts. LAB group can be isolated and characterized from traditional dairy sources. This study aimed at isolating, identifying, and in vitro characterizing (low pH/high bile salt tolerance, antibacterial activity, and antibiotic susceptibility) LAB strains from traditional Iranian dairy products. Materials and Methods: Isolated strains were identified by Gram staining, catalase assay, and 3 molecular identification methods; namely, (GTG) 5-PCR fingerprinting, ARDRA, and 16S rDNA gene sequencing...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29238458/bacteriocinogenic-potential-and-virulence-traits-of-enterococcus-faecium-and-e-faecalis-isolated-from-human-milk
#8
Soodabeh Khalkhali, Naheed Mojgani
Background and Objectives: Human milk is a continuous supply of Lactic Acid bacteria (LAB), including enterococci with probiotic potentials. The aim of this study was to analyze two Enterococcus species, isolated from human milk for their probiotic potential, bacteriocin producing ability and virulence traits. Materials and Methods: Enterococcus faecium TA0033 and E. faecalis TA102 were tested for acid and bile tolerance, survival in simulated gastric and intestinal conditions...
August 2017: Iranian Journal of Microbiology
https://www.readbyqxmd.com/read/29238325/probiotic-bacteria-alter-pattern-recognition-receptor-expression-and-cytokine-profile-in-a-human-macrophage-model-challenged-with-candida-albicans-and-lipopolysaccharide
#9
Victor H Matsubara, Karin H Ishikawa, Ellen S Ando-Suguimoto, Bruno Bueno-Silva, Atlas E M Nakamae, Marcia P A Mayer
Probiotics are live microorganisms that confer benefits to the host health. The infection rate of potentially pathogenic organisms such as Candida albicans, the most common agent associated with mucosal candidiasis, can be reduced by probiotics. However, the mechanisms by which the probiotics interfere with the immune system are largely unknown. We evaluated the effect of probiotic bacteria on C. albicans challenged human macrophages. Macrophages were pretreated with lactobacilli alone (Lactobacillus rhamnosus LR32, Lactobacillus casei L324m, or Lactobacillus acidophilus NCFM) or associated with Escherichia coli lipopolysaccharide (LPS), followed by the challenge with C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29236734/freeze-dried-lactobacillus-plantarum-299v-increases-iron-absorption-in-young-females-double-isotope-sequential-single-blind-studies-in-menstruating-women
#10
Michael Hoppe, Gunilla Önning, Lena Hulthén
BACKGROUND: The probiotic strain Lactobacillus plantarum 299v has earlier been shown to increase iron absorption when added to foods. However, it is not known if the same probiotic strain in a freeze-dried format included in a capsule increases the iron absorption. OBJECTIVE: The aim of this study was to test the hypotheses that non-heme iron absorption from a light meal is promoted by a simultaneous intake of freeze-dried Lactobacillus plantarum 299v (Lp299v, DSM 9843)...
2017: PloS One
https://www.readbyqxmd.com/read/29236340/flow-cytometry-a-versatile-technology-for-specific-quantification-and-viability-assessment-of-microorganisms-in-multi-strain-probiotic-products
#11
Camille Chiron, Thomas A Tompkins, Pierre Burguière
AIMS: Classical microbiology techniques are the gold standard for probiotic enumeration. However, these techniques are limited by parameters of time, specificity and incapacity to detect viable but non-culturable (VBNC) microorganisms and non-viable cells. The aim of the study was to evaluate flow cytometry as a novel method for the specific quantification of viable and non-viable probiotics in multi-strain products. METHODS AND RESULTS: Custom polyclonal antibodies were produced against five probiotic strains from different species (Bifidobacterium bifidum R0071, Bifidobacterium longum ssp...
December 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29234255/influence-of-orally-administered-probiotic-lactobacillus-strains-on-vaginal-microbiota-in-women-with-breast-cancer-during-chemotherapy-a-randomized-placebo-controlled-double-blinded-pilot-study
#12
Julian Marschalek, Alex Farr, Marie-Louise Marschalek, Konrad J Domig, Wolfgang Kneifel, Christian F Singer, Herbert Kiss, Ljubomir Petricevic
Background: Due to chemotherapy and estrogen deprivation therapy, genitourinary syndrome of menopause is a common condition in breast cancer patients. We aimed to determine the effect of an orally administered Lactobacillus preparation on the vaginal microbiota in breast cancer patients. Methods: Postmenopausal breast cancer patients receiving chemotherapy, with vaginal atrophy and an intermediate vaginal microbiota (Nugent score 4-6), were either randomized to the intervention group receiving probiotic capsules of 4 Lactobacillus species or to the control group receiving placebo twice daily for 2 weeks...
October 2017: Breast Care
https://www.readbyqxmd.com/read/29234140/altered-gut-microbiota-profiles-in-sows-and-neonatal-piglets-associated-with-porcine-epidemic-diarrhea-virus-infection
#13
Deping Song, Qi Peng, Yanjun Chen, Xinrong Zhou, Fanfan Zhang, Anqi Li, Dongyan Huang, Qiong Wu, Yu Ye, Houjun He, Leyi Wang, Yuxin Tang
Porcine epidemic diarrhea virus (PEDV) is a devastating cause of diarrhea in pigs worldwide. Most of studies have focused on molecular and pathogenic characterization of PEDV, whereas there were limited studies in understanding the role of gut microbiota (GM) in viral-associated diarrhea. Here, using the Illumina MiSeq platform, we examined and compared the impact of PEDV infection on the GM of sows and their piglets less than 10 days old. Our results showed that PEDV caused alternations in the structure and abundance of GM from levels of phylum to genus, and even species...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29234060/heat-killed-lactobacillus-casei-confers-broad-protection-against-influenza-a-virus-primary-infection-and-develops-heterosubtypic-immunity-against-future-secondary-infection
#14
Yu-Jin Jung, Young-Tae Lee, Vu Le Ngo, Young-Hee Cho, Eun-Ju Ko, Sung-Moon Hong, Ki-Hye Kim, Ji-Hun Jang, Joon-Suk Oh, Min-Kyung Park, Cheol-Hyun Kim, Jun Sun, Sang-Moo Kang
Lactic acid bacteria (LAB) are the common probiotics. Here, we investigated the antiviral protective effects of heat-killed LAB strain Lactobacillus casei DK128 (DK128) on influenza viruses. Intranasal treatment of mice with DK128 conferred protection against different subtypes of influenza viruses by lessening weight loss and lowering viral loads. Protection via heat-killed DK128 was correlated with an increase in alveolar macrophage cells in the lungs and airways, early induction of virus specific antibodies, reduced levels of pro-inflammatory cytokines and innate immune cells...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233677/alzheimer-s-disease-and-gut-microbiota-modifications-the-long-way-between-preclinical-studies-and-clinical-evidence
#15
REVIEW
Cesare Mancuso, Rosaria Santangelo
Recent studies have suggested the role of an infectious component in the pathogenesis of Alzheimer's disease (AD). In light of this, research has focused on some bacteria constituting the intestinal microbial flora which can produce amyloid. Once generated, the latter hypothetically triggers a systemic inflammatory response which compromises complex brain functions, such as learning and memory. Clinical studies have shown that, in cognitively impaired elderly patients with brain amyloidosis, there is lower abundance in the gut of E...
December 9, 2017: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29232932/adjuvant-probiotics-and-the-intestinal-microbiome-enhancing-vaccines-and-immunotherapy-outcomes
#16
REVIEW
Luis Vitetta, Emma Tali Saltzman, Michael Thomsen, Tessa Nikov, Sean Hall
Immune defence against pathogenic agents comprises the basic premise for the administration of vaccines. Vaccinations have hence prevented millions of infectious illnesses, hospitalizations and mortality. Acquired immunity comprises antibody and cell mediated responses and is characterized by its specificity and memory. Along a similar congruent yet diverse mode of disease prevention, the human host has negotiated from in utero and at birth with the intestinal commensal bacterial cohort to maintain local homeostasis in order to achieve immunological tolerance in the new born...
December 11, 2017: Vaccines
https://www.readbyqxmd.com/read/29232930/effects-of-bacterial-fermentation-on-the-biochemical-constituents-and-antioxidant-potential-of-fermented-and-unfermented-soybeans-using-probiotic-bacillus-subtilis-kctc-13241
#17
Muhammad Waqas Ali, Il-Doo Kim, Saqib Bilal, Raheem Shahzad, Muhammad Tariq Saeed, Bishnu Adhikari, Rizwana Begum Syed Nabi, Jeong Rae Kyo, Dong-Hyun Shin
Fermented soybeans, cheonggukjang (CKJ), are considered to be more wholesome than soybeans in Korea. To select the best soybean cultivar for making functional CKJ, a comparison was made between the biological activities of four soybean cultivars in their unfermented soybean (UFS) and CKJ states. Changes in 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assays, superoxide dismutase (SOD)-like activity, total phenolic compounds, total amino acids, and isoflavones were investigated...
December 11, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29232851/a-novel-prebiotic-blend-product-prevents-irritable-bowel-syndrome-in-mice-by-improving-gut-microbiota-and-modulating-immune-response
#18
Qian Chen, Yiping Ren, Jihong Lu, Mark Bartlett, Lei Chen, Yan Zhang, Xiaokui Guo, Chang Liu
Irritable bowel syndrome (IBS) is the most common functional gastrointestinal disorder yet it still lacks effective prevention therapies. The aim of this study is to determine whether a novel prebiotic blend (PB) composed of fructo-oligosaccharide (FOS), galactooligosaccharide (GOS), inulin and anthocyanins could be effective in preventing the development of IBS. We explored the possible mechanisms both in animal and in cells. Post-infectious IBS models in C57BL/6 mice were established and were pretreated with the PB, PB and probiotic strains 8 weeks in advance of infection...
December 9, 2017: Nutrients
https://www.readbyqxmd.com/read/29232848/a-metagenomic-and-in-silico-functional-prediction-of-gut-microbiota-profiles-may-concur-in-discovering-new-cystic-fibrosis-patient-targeted-probiotics
#19
Pamela Vernocchi, Federica Del Chierico, Andrea Quagliariello, Danilo Ercolini, Vincenzina Lucidi, Lorenza Putignani
Cystic fibrosis (CF) is a life-limiting hereditary disorder that results in aberrant mucosa in the lungs and digestive tract, chronic respiratory infections, chronic inflammation, and the need for repeated antibiotic treatments. Probiotics have been demonstrated to improve the quality of life of CF patients. We investigated the distribution of gut microbiota (GM) bacteria to identify new potential probiotics for CF patients on the basis of GM patterns. Fecal samples of 28 CF patients and 31 healthy controls (HC) were collected and analyzed by 16S rRNA-based pyrosequencing analysis of GM, to produce CF-HC paired maps of the distribution of operational taxonomic units (OTUs), and by Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) for Kyoto Encyclopedia of Genes and Genomes (KEGG) biomarker prediction...
December 9, 2017: Nutrients
https://www.readbyqxmd.com/read/29231922/gene-expression-profiling-in-the-intestinal-mucosa-of-obese-rats-administered-probiotic-bacteria
#20
Julio Plaza-Díaz, Cándido Robles-Sánchez, Francisco Abadía-Molina, María José Sáez-Lara, Laura María Vilchez-Padial, Ángel Gil, Carolina Gómez-Llorente, Luis Fontana
We investigated whether the administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 modulate the expression of genes in the intestinal mucosa of obese Zucker rats. Forty-eight Zucker-Leprfa/fa and 16 Zucker lean Lepr+/fa rats were used. Eight Zucker lean Lepr+/fa and 8 Zucker-Leprfa/fa rats were euthanized as a reference. The remaining 40 Zucker-Leprfa/fa rats were then assigned to receive 1010 colony forming units (CFU) of one of the three probiotic strains, a mixture of L...
December 12, 2017: Scientific Data
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