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https://www.readbyqxmd.com/read/27634912/oncogenic-microrna-4534-regulates-pten-pathway-in-prostate-cancer
#1
Hannah Nip, Altaf A Dar, Sharanjot Saini, Melissa Colden, Shahryari Varahram, Harshika Chowdhary, Soichiro Yamamura, Yozo Mitsui, Yuichiro Tanaka, Taku Kato, Yutaka Hashimoto, Marisa Shiina, Priyanka Kulkarni, Pritha Dasgupta, Mitsuho Imai-Sumida, Z Laura Tabatabai, Kirsten Greene, Guoren Deng, Rajvir Dahiya, Shahana Majid
Prostate carcinogenesis involves alterations in several signaling pathways, the most prominent being the PI3K/AKT pathway. This pathway is constitutively active and drives prostate cancer (PCa) progression to advanced metastatic disease. PTEN, a critical tumor and metastasis suppressor gene negatively regulates cell survival, proliferation, migration and angiogenesis via the PI3K/Akt pathway. PTEN is mutated, downregulated/dysfunctional in many cancers and its dysregulation correlates with poor prognosis in PCa...
September 15, 2016: Oncotarget
https://www.readbyqxmd.com/read/27509989/roles-of-microrna21-and-microrna29a-in-regulating-cell-adhesion-related-genes-in-bone-metastasis-secondary-to-prostate-cancer
#2
Maisarah Mohamad, Norhazlina Abdul Wahab, Rosna Yunus, Nor Azian Abdul Murad, Zulkifli Md Zainuddin, Murali Sundaram, Norfilza Mohd Mokhtar
BACKGROUND: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be downregulated in clinical samples, most likely due to the posttranscriptional modification by microRNAs. Targeted genes would be upregulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27231600/lensfree-diffractive-tomography-for-the-imaging-of-3d-cell-cultures
#3
F Momey, A Berdeu, T Bordy, J-M Dinten, F Kermarrec Marcel, N Picollet-D'hahan, X Gidrol, C Allier
New microscopes are needed to help realize the full potential of 3D organoid culture studies. In order to image large volumes of 3D organoid cultures while preserving the ability to catch every single cell, we propose a new imaging platform based on lensfree microscopy. We have built a lensfree diffractive tomography setup performing multi-angle acquisitions of 3D organoid culture embedded in Matrigel and developed a dedicated 3D holographic reconstruction algorithm based on the Fourier diffraction theorem...
March 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27060259/oxidative-phosphorylation-and-mitochondrial-function-differ-between-human-prostate-tissue-and-cultured-cells
#4
Bernd Schöpf, Georg Schäfer, Anja Weber, Heribert Talasz, Iris E Eder, Helmut Klocker, Erich Gnaiger
Altered mitochondrial metabolism plays a pivotal role in the development and progression of various diseases, including cancer. Cell lines are frequently used as models to study mitochondrial (dys)function but little is known about their mitochondrial respiration and metabolic properties in comparison to the primary tissue of origin. We have developed a method for assessment of oxidative phosphorylation in prostate tissue samples of only 2 mg wet weight using high-resolution respirometry. Reliable protocols were established to investigate the respiratory activity of different segments of the mitochondrial electron transfer system in mechanically permeabilized tissue biopsies...
April 6, 2016: FEBS Journal
https://www.readbyqxmd.com/read/25756394/tgf%C3%AE-induced-phosphorylation-of-par6-promotes-migration-and-invasion-in-prostate-cancer-cells
#5
Y Mu, G Zang, U Engström, C Busch, M Landström
BACKGROUND: The Par complex - comprising partition-defective 6 (Par6), Par3, and atypical protein kinase C (aPKC) - is crucial for cell polarisation, the loss of which contributes to cancer progression. Transforming growth factor β (TGFβ)-induced phosphorylation of Par6 on the conserved serine 345 is implicated in epithelial-to-mesenchymal transition (EMT) in breast cancer. Here we investigated the importance of phosphorylated Par6 in prostate cancer. METHODS: We generated a p-Par6(345)-specific antibody and verified its specificity in vitro...
March 31, 2015: British Journal of Cancer
https://www.readbyqxmd.com/read/25446255/vip-induces-nf-%C3%AE%C2%BAb1-nuclear-localisation-through-different-signalling-pathways-in-human-tumour-and-non-tumour-prostate-cells
#6
Ana B Fernández-Martínez, María J Carmena, Ana M Bajo, Eva Vacas, Manuel Sánchez-Chapado, Juan C Prieto
The nuclear factor κB (NF-κB) is a powerful activator of angiogenesis, invasion and metastasis. Transactivation and nuclear localisation of NF-κB is an index of recurrence in prostate cancer. Vasoactive intestinal peptide (VIP) exerts similar effects in prostate cancer models involving increased expression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) which are related to NF-κB transactivation. Here we studied differential mechanisms of VIP-induced NF-κB transactivation in non-tumour RWPE-1 and tumour LNCaP and PC3 human prostate epithelial cells...
February 2015: Cellular Signalling
https://www.readbyqxmd.com/read/25410184/cystine-dimethyl-ester-induces-apoptosis-through-regulation-of-pkc-%C3%AE-and-pkc-%C3%AE%C2%B5-in-prostate-cancer-cells
#7
Nilgun Gurbuz, Margaret A Park, Paul Dent, Asim B Abdel Mageed, Suresh C Sikka, Asli Baykal
Protein kinase C-δ (PKC-δ) and PKC-ε are reported to be effective in cancer prevention via S-thiolation-mediated mechanisms. This may be through stimulation of the pro-apoptotic, tumor-suppressive isozyme PKC-δ and/or inactivation of the growth stimulatory, oncogenic isozyme PKC-ε. We investigated oxidative regulatory responses of PKC-δ and PKC-ε to cystine dimethyl ester (CDME), a metabolic precursor of cystine, which, by inducing release of cellular cystine stimulates apoptosis in different prostate cancer cells, PC3 and LNCaP, compared to normal RWPE1 cells...
2015: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/25237656/a-milk-protein-casein-as-a-proliferation-promoting-factor-in-prostate-cancer-cells
#8
Sung-Woo Park, Joo-Young Kim, You-Sun Kim, Sang Jin Lee, Sang Don Lee, Moon Kee Chung
PURPOSE: Despite most epidemiologic studies reporting that an increase in milk intake affects the growth of prostate cancer, the results of experimental studies are not consistent. In this study, we investigated the proliferation of prostate cancer cells treated with casein, the main protein in milk. MATERIALS AND METHODS: Prostate cancer cells (LNCaP and PC3), lung cancer cells (A459), stomach cancer cells (SNU484), breast cancer cells (MCF7), immortalized human embryonic kidney cells (HEK293), and immortalized normal prostate cells (RWPE1) were treated with either 0...
August 2014: World Journal of Men's Health
https://www.readbyqxmd.com/read/25227780/dna-fragmentation-caspase-3-and-prostate-specific-antigen-genes-expression-induced-by-arsenic-cadmium-and-chromium-on-nontumorigenic-human-prostate-cells
#9
Hend M Abo El-Atta, Amal A El-Bakary, Afaf M Attia, Ahmed Lotfy, Shery S Khater, Ayman Z Elsamanoudy, Hussein Abdelaziz Abdalla
Prostate cancer is one of the most common cancers and the second cause of cancer-related deaths among men. Metals are recognized as chemical carcinogens where chronic exposures to such metals are implicated in the development of cancer, including prostate cancer. This in vitro study demonstrates the relative death sensitivity of prostatic (RWPE-1) cells to arsenic (As), cadmium (Cd), and chromium (Cr) as environmental pollutants through its apoptotic effects and the effect of these chemicals on prostate-specific antigen (PSA) gene expression as a marker for their carcinogecity...
December 2014: Biological Trace Element Research
https://www.readbyqxmd.com/read/25194810/ubiquitin-c-terminal-hydrolase-l3-regulates-emt-process-and-cancer-metastasis-in-prostate-cell-lines
#10
Hyun Min Song, Jae Eun Lee, Jung Hwa Kim
Ubiquitin C-terminal hydrolase-L3 (UCH-L3) is among the deubiquitinating enzymes (DUBs) that cleave ubiquitin (Ub) from Ub precursors or protein substrates. Many DUBs have been shown to participate in cancer progression in various tissues. However, the mechanism and role of UCH-L3 in carcinogenesis has largely been unknown until recently. Here we investigated the implication of UCH-L3 in prostate cancer progression. Interestingly, UCH-L3 is upregulated in normal or non-metastatic prostate cancer cells and is downregulated in metastatic prostate cancer cell lines...
September 26, 2014: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/24886089/dtx3l-and-artd9-inhibit-irf1-expression-and-mediate-in-cooperation-with-artd8-survival-and-proliferation-of-metastatic-prostate-cancer-cells
#11
Samia B Bachmann, Sandra C Frommel, Rosalba Camicia, Hans C Winkler, Raffaella Santoro, Paul O Hassa
BACKGROUND: Prostate cancer (PCa) is one of the leading causes of cancer-related mortality and morbidity in the aging male population and represents the most frequently diagnosed malignancy in men around the world. The Deltex (DTX)-3-like E3 ubiquitin ligase (DTX3L), also known as B-lymphoma and BAL-associated protein (BBAP), was originally identified as a binding partner of the diphtheria-toxin-like macrodomain containing ADP-ribosyltransferase-9 (ARTD9), also known as BAL1 and PARP9...
May 27, 2014: Molecular Cancer
https://www.readbyqxmd.com/read/24753248/determination-of-carbohydrate-structure-recognized-by-prostate-specific-f77-monoclonal-antibody-through-expression-analysis-of-glycosyltransferase-genes
#12
Motohiro Nonaka, Michiko N Fukuda, Chao Gao, Zhen Li, Hongtao Zhang, Mark I Greene, Donna M Peehl, Ten Feizi, Minoru Fukuda
This study reports the determination of the carbohydrate epitope of monoclonal antibody F77 previously raised against human prostate cancer PC-3 cells (Zhang, G., Zhang, H., Wang, Q., Lal, P., Carroll, A. M., de la Llera-Moya, M., Xu, X., and Greene, M. I. (2010) Proc. Natl. Acad. Sci. U. S. A. 107, 732-737). We performed a series of co-transfections using mammalian expression vectors encoding specific glycosyltransferases. We thereby identified branching enzymes and FUT1 (required for Fucα1→2Gal linkage) as being essential for F77 antigen formation...
June 6, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24666255/formononetin-induces-apoptosis-in-pc-3-prostate-cancer-cells-through-enhancing-the-bax-bcl-2-ratios-and-regulating-the-p38-akt-pathway
#13
Xing Zhang, Lingyun Bi, Yu Ye, Jian Chen
Formononetin (FN), a bioactive component extracted from the red clover (Trifolium pratense L.), has been long used for treating carcinomas in China. In the present study, we aim to investigate the potential therapeutical effects of FN on cell line of prostatic adenocarcinoma (PC-3) and human prostate epithelial cells (RWPE1). These findings indicated that FN significantly inhibited the cell growth of PC-3 in a dose-dependent manner, but no such effect was observed in RWPE1 cells. The apoptotic counts were effectively increased following the treatments as shown in flow cytometry...
2014: Nutrition and Cancer
https://www.readbyqxmd.com/read/24583925/tr4-nuclear-receptor-functions-as-a-tumor-suppressor-for-prostate-tumorigenesis-via-modulation-of-dna-damage-repair-system
#14
Shin-Jen Lin, Soo Ok Lee, Yi-Fen Lee, Hiroshi Miyamoto, Dong-Rong Yang, Gonghui Li, Chawnshang Chang
Testicular nuclear receptor 4 (TR4), a member of the nuclear receptor superfamily, plays important roles in metabolism, fertility and aging. The linkage of TR4 functions in cancer progression, however, remains unclear. Using three different mouse models, we found TR4 could prevent or delay prostate cancer (PCa)/prostatic intraepithelial neoplasia development. Knocking down TR4 in human RWPE1 and mouse mPrE normal prostate cells promoted tumorigenesis under carcinogen challenge, suggesting TR4 may play a suppressor role in PCa initiation...
June 2014: Carcinogenesis
https://www.readbyqxmd.com/read/24528787/enhanced-detection-of-metastatic-prostate-cancer-cells-in-human-plasma-with-lipid-bodies-staining
#15
Ranjana Mitra, Oscar B Goodman, Thuc T Le
BACKGROUND: Reprogramming of energy metabolism of malignant cancer cells confers competitive advantage in growth environments with limited resources. However, not every process of cancer development is associated with competition for resources. During hematogenous transport, cancer cells are exposed to high levels of oxygen and nutrients. Does energy metabolism of cancer cells change as a function of exposure to the bloodstream? Could such changes be exploited to improve the detection of circulating tumor cells (CTC)? These questions have clinical significance, but have not yet been sufficiently examined...
2014: BMC Cancer
https://www.readbyqxmd.com/read/24375805/expression-profiles-and-functional-associations-of-endogenous-androgen-receptor-and-caveolin-1-in-prostate-cancer-cell-lines
#16
Nigel C Bennett, John D Hooper, David W Johnson, Glenda C Gobe
BACKGROUND: In prostate cancer (PCa) patients, the protein target for androgen deprivation and blockade therapies is androgen receptor (AR). AR interacts with many proteins that function to either co-activate or co-repress its activity. Caveolin-1 (Cav-1) is not found in normal prostatic epithelium, but is found in PCa, and may be an AR co-regulator protein. METHODS: We investigated cell line-specific signatures and associations of endogenous AR and Cav-1 in six PCa cell lines of known androgen sensitivity: LNCaP (androgen sensitive); 22Rv1 (androgen responsive); PC3, DU145, and ALVA41 (androgen non-reliant); and RWPE1 (non-malignant)...
May 2014: Prostate
https://www.readbyqxmd.com/read/23792589/chromosomal-structural-variations-during-progression-of-a-prostate-epithelial-cell-line-to-a-malignant-metastatic-state-inactivate-the-nf2-nipsnap1-ugt2b17-and-lpin2-genes
#17
Ankit Malhotra, Yoshiyuki Shibata, Ira M Hall, Anindya Dutta
Prostate cancer is the second highest cause of male cancer deaths in the United States. A significant number of tumors advance to a highly invasive and metastatic stage, which is typically resistant to traditional cancer therapeutics. In order to identify chromosomal structural variants that may contribute to prostate cancer progression we sequenced the genomes of a HPV-18 immortalized nonmalignant human prostate epithelial cell line, RWPE1, and compared it to its malignant, metastatic derivative, WPE1-NB26...
September 2013: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/23747358/label-free-analysis-of-prostate-acini-like-3d-structures-by-lensfree-imaging
#18
Monika E Dolega, Cédric Allier, Srikanth Vinjimore Kesavan, Sophie Gerbaud, Frédérique Kermarrec, Pierre Marcoux, Jean-Marc Dinten, Xavier Gidrol, Nathalie Picollet-D'Hahan
We present a lensfree imaging method to analyze polarity in RWPE1 prostate epithelial cells that form polarized acini with lumen under standard tridimensional (3D) culture conditions. The first event in epithelial carcinogenesis is loss of polarity, followed by uncontrolled proliferation leading to metastasis. We demonstrate that it is possible to use optical signatures to discriminate 3D objects with distinct polarities in a large field of view. The three metrics we present here are designed as image processing tools to discriminate acini from spheroids without any 3D reconstruction...
November 15, 2013: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/23669876/ck2-ncor-signaling-cascade-promotes-prostate-tumorigenesis
#19
Jung-Yoon Yoo, Beom Jin Lim, Hyo-Kyoung Choi, Soon Won Hong, Ho Sung Jang, Changsoo Kim, Kyung-Hee Chun, Kyung-Chul Choi, Ho-Geun Yoon
The aberrant expressions of casein kinase 2 (CK2) was found in prostate cancer patient and cell lines, but little is known of the detailed mechanisms implicated in prostate tumorigenesis. In this study, we report that both CK2 activity and CK2-mediated NCoR phosphorylation are significantly elevated in the androgen-independent prostate cancer cell line DU145 and PC-3 compared with RWPE1 and LNCaP cells. Increased phosphorylation inversely correlates with the mRNA level of the NCoR-regulated gene, interferon-γ-inducible protein 10 (IP-10)...
July 2013: Oncotarget
https://www.readbyqxmd.com/read/23650590/oxidative-stress-adaptation-in-aggressive-prostate-cancer-may-be-counteracted-by-the-reduction-of-glutathione-reductase
#20
Mariana Freitas, Inês Baldeiras, Teresa Proença, Vera Alves, Anabela Mota-Pinto, Ana Sarmento-Ribeiro
Oxidative stress has been associated with prostate cancer development and progression due to an increase of reactive oxygen species (ROS). However, the mechanisms whereby ROS and the antioxidant system participate in cancer progression remain unclear. In order to clarify the influence of oxidative stress in prostate cancer progression, we performed this study in two human prostate cancer cell lines, PC3 and HPV10 (from metastasis and from localized cancer, respectively) and RWPE1 cells derived from normal prostate epithelium...
2012: FEBS Open Bio
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