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https://www.readbyqxmd.com/read/29891991/extracellular-vesicles-with-altered-tetraspanin-cd9-and-cd151-levels-confer-increased-prostate-cell-motility-and-invasion
#1
Joshua S Brzozowski, Danielle R Bond, Helen Jankowski, Belinda J Goldie, Rachel Burchell, Crystal Naudin, Nathan D Smith, Christopher J Scarlett, Martin R Larsen, Matthew D Dun, Kathryn A Skelding, Judith Weidenhofer
To facilitate intercellular communication, cells release nano-sized, extracellular vesicles (EVs) to transfer biological cargo to both local and distant sites. EVs are enriched in tetraspanins, two of which (CD9 and CD151) have altered expression patterns in many solid tumours, including prostate cancer, as they advance toward metastasis. We aimed to determine whether EVs from prostate cells with altered CD9 and CD151 expression could influence cellular behaviour and increase the metastatic capabilities of non-tumourigenic prostate cells...
June 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29556062/androgen-receptor-modulation-following-combination-exposure-to-brominated-flame-retardants
#2
Joubert Banjop Kharlyngdoh, Ajay Pradhan, Per-Erik Olsson
Endocrine disrupting compounds can interfere with androgen receptor (AR) signaling and disrupt steroidogenesis leading to reproductive failure. The brominated flame-retardant (BFR) 1, 2-dibromo-4-(1, 2-dibromoethyl) cyclohexane (TBECH), is an agonist to human, chicken and zebrafish AR. Recently another group of alternative BFRs, allyl 2, 4, 6-tribromophenyl ether (ATE), and 2, 3-dibromopropyl 2, 4, 6-tribromophenyl ether (DPTE) along with its metabolite 2-bromoallyl 2, 4, 6-tribromophenyl ether (BATE) were identified as potent human AR antagonists...
March 19, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29500887/tgf%C3%AE-induced-epithelial-to-mesenchymal-transition-in-prostate-cancer-cells-is-mediated-via-trpm7-expression
#3
Yuyang Sun, Anne Schaar, Pramod Sukumaran, Archana Dhasarathy, Brij B Singh
Growth factors, such as the transforming growth factor beta (TGFβ), play an important role in promoting metastasis of prostate cancer, thus understanding how TGFβ could induce prostate cancer cell migration may enable us to develop targeted strategies for treatment of advanced metastatic prostate cancer. To more clearly define the mechanism(s) involved in prostate cancer cell migration, we undertook a series of studies utilizing non-malignant prostate epithelial cells RWPE1 and prostate cancer DU145 and PC3 cells...
June 2018: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/29416746/mir-518f-5p-decreases-tetraspanin-cd9-protein-levels-and-differentially-affects-non-tumourigenic-prostate-and-prostate-cancer-cell-migration-and-adhesion
#4
Danielle R Bond, Crystal Naudin, Adam P Carroll, Belinda J Goldie, Joshua S Brzozowski, Helen M Jankowski, Murray J Cairns, Leonie K Ashman, Christopher J Scarlett, Judith Weidenhofer
Tetraspanin CD9 is generally considered to be a metastasis suppressor, with decreased levels associated with progression and metastasis in many advanced stage cancers. Little is known about the cause of CD9 dysregulation in prostate cancer, however there are several miRNA-binding sites in the 3´UTR of the transcript suggesting it could be post-transcriptionally regulated. Using microarrays and luciferase assays in tumourigenic and non-tumourigenic prostate cell lines we identified miR-518f-5p as a regulator of the CD9 3'UTR gene expression, and decreased expression of endogenous CD9 in non-tumorigenic prostate RWPE1 and prostate cancer DU145 cells...
January 5, 2018: Oncotarget
https://www.readbyqxmd.com/read/29341212/differential-role-of-pten-in-transforming-growth-factor-%C3%AE-tgf-%C3%AE-effects-on-proliferation-and-migration-in-prostate-cancer-cells
#5
Mawiyah N Kimbrough-Allah, Ana C Millena, Shafiq A Khan
BACKGROUND: Transforming growth factor-β (TGF-β) acts as a tumor suppressor in normal epithelial cells but as a tumor promoter in advanced prostate cancer cells. PI3-kinase pathway mediates TGF-β effects on prostate cancer cell migration and invasion. PTEN inhibits PI3-kinase pathway and is frequently mutated in prostate cancers. We investigated possible role(s) of PTEN in TGF-β effects on proliferation and migration in prostate cancer cells. METHODS: Expression of PTEN mRNA and proteins were determined using RT-PCR and Western blotting in RWPE1 and DU145 cells...
January 16, 2018: Prostate
https://www.readbyqxmd.com/read/28718667/hydroxytyrosol-induces-apoptosis-and-cell-cycle-arrest-and-suppresses-multiple-oncogenic-signaling-pathways-in-prostate-cancer-cells
#6
Haseeb Zubair, Arun Bhardwaj, Aamir Ahmad, Sanjeev Kumar Srivastava, Mohammad Aslam Khan, Girijesh Kumar Patel, Seema Singh, Ajay Partap Singh
SCOPE: Hydroxytyrosol (HT), a polyphenol from olives, is a potential anticancer agent. This study was designed to evaluate the anticancer activity of HT against prostate cancer cells, and the mechanism thereof. METHODS AND RESULTS: Treatment of LNCaP and C4-2 prostate cancer cells with HT resulted in a dose-dependent inhibition of proliferation. This was in contrast to HT's ineffectiveness against normal prostate epithelial cells RWPE1 and PWLE2, suggesting cancer-cell-specific effect...
August 2017: Nutrition and Cancer
https://www.readbyqxmd.com/read/28532481/identification-of-endonuclease-domain-containing-1-as-a-novel-tumor-suppressor-in-prostate-cancer
#7
Jianguang Qiu, Shubin Peng, Jie Si-Tu, Cheng Hu, Wentao Huang, Yunhua Mao, Wenhan Qiu, Ke Li, Dejuan Wang
BACKGROUND: Endonuclease domain containing 1 (ENDOD1) is implicated in tumorigenesis and aggressiveness of multiple tumors. In this study, we aimed to investigate the role of ENDOD1 in prostate cancer (PCa). METHODS: Immunohistochemistry were performed in 30 cases of benign prostatic hyperplasia (BPH) and 50 cases of PCa to identify its association with clinicopathological characteristics. Real-time PCR and western blot were used to detect ENDOD1 mRNA and protein expression in normal prostatic epithelial and PCa cell lines...
May 22, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28481876/oncogenic-activity-of-amplified-miniature-chromosome-maintenance-8-in-human-malignancies
#8
D-M He, B-G Ren, S Liu, L-Z Tan, K Cieply, G Tseng, Y P Yu, J-H Luo
Miniature chromosome maintenance (MCM) proteins play critical roles in DNA replication licensing, initiation and elongation. MCM8, one of the MCM proteins playing a critical role in DNA repairing and recombination, was found to have overexpression and increased DNA copy number in a variety of human malignancies. The gain of MCM8 is associated with aggressive clinical features of several human cancers. Increased expression of MCM8 in prostate cancer is associated with cancer recurrence. Forced expression of MCM8 in RWPE1 cells, the immortalized but non-transformed prostate epithelial cell line, exhibited fast cell growth and transformation, while knock down of MCM8 in PC3, DU145 and LNCaP cells induced cell growth arrest, and decreased tumour volumes and mortality of severe combined immunodeficiency mice xenografted with PC3 and DU145 cells...
June 22, 2017: Oncogene
https://www.readbyqxmd.com/read/28440481/the-specific-killing-effect-of-matrine-on-castration-resistant-prostate-cancer-cells-by-targeting-the-akt-foxo3a-signaling-pathway
#9
Shoumin Bai, Ting Chen, Xiaoli Yu, Ming Luo, Xianju Chen, Chunhao Lin, Yiming Lai, Hai Huang
Matrine, a Sophora alkaloid, exhibits antiproliferative and anti-carcinogenic activities through several mechanisms. In a previous study, we found that matrine could effectively inhibit the proliferation of castration-resistant prostate cancer (CRPC). In the present study, the effect of matrine and LY294002 on the expression of the Akt/FoxO3a signaling pathway was examined by western blot analyses and RT-PCR. We discovered that matrine significantly inhibited the proliferation of both prostate cancer cell line PC-3 and prostate epithelial cell line RWPE1, induced apoptosis and induced cell cycle arrest...
May 2017: Oncology Reports
https://www.readbyqxmd.com/read/28408625/ets-e26-transformation-specific-up-regulation-of-the-transcriptional-co-activator-taz-promotes-cell-migration-and-metastasis-in-prostate-cancer
#10
Chen-Ying Liu, Tong Yu, Yuji Huang, Long Cui, Wanjin Hong
Prostate cancer is a very common malignant disease and a leading cause of death for men in the Western world. Tumorigenesis and progression of prostate cancer involves multiple signaling pathways, including the Hippo pathway. Yes-associated protein (YAP) is the downstream transcriptional co-activator of the Hippo pathway, is overexpressed in prostate cancer, and plays a vital role in the tumorigenesis and progression of prostate cancer. However, the role of the YAP paralog and another downstream effector of the Hippo pathway, transcriptional co-activator with PDZ-binding motif (TAZ), in prostate cancer has not been fully elucidated...
June 2, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28385910/metformin-reduces-prostate-tumor-growth-in-a-diet-dependent-manner-by-modulating-multiple-signaling-pathways
#11
André Sarmento-Cabral, Fernando L-López, Manuel D Gahete, Justo P Castaño, Raúl M Luque
Prostate-cancer is strongly influenced by obesity, wherein metformin could represent a promising treatment; however, the endocrine metabolic/cellular/molecular mechanisms underlying these associations and effects are still unclear. To determine the beneficial antitumoral effects of metformin on prostate cancer progression/aggressiveness and the relative contribution of high-fat diet (HFD; independently of obesity), we used HFD-fed immunosuppressed mice inoculated with PC3 cells (which exhibited partial resistance to diet-induced obesity) compared with low-fat diet (LFD)-fed control mice...
July 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28374086/notch-4-silencing-inhibits-prostate-cancer-growth-and-emt-via-the-nf-%C3%AE%C2%BAb-pathway
#12
Jianwei Zhang, Youlin Kuang, Yan Wang, Quanquan Xu, Qinghua Ren
Epithelial-mesenchymal transition (EMT) is implicated in the metastasis of human prostate cancer (PCa). Notch signaling has been established as a regulator of EMT. Notch-4 has emerged as a mammary proto-oncogene and a target in several cancers. However, the role and the mechanism of action of Notch-4 in PCa are still unclear. In the present study, we first observed a marked increase in Notch-4 expression in the PCa cell lines DU145, PC3 and LnCAP compared with the non-malignant prostate epithelial cell line RWPE1...
June 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27634912/oncogenic-microrna-4534-regulates-pten-pathway-in-prostate-cancer
#13
Hannah Nip, Altaf A Dar, Sharanjot Saini, Melissa Colden, Shahryari Varahram, Harshika Chowdhary, Soichiro Yamamura, Yozo Mitsui, Yuichiro Tanaka, Taku Kato, Yutaka Hashimoto, Marisa Shiina, Priyanka Kulkarni, Pritha Dasgupta, Mitsuho Imai-Sumida, Z Laura Tabatabai, Kirsten Greene, Guoren Deng, Rajvir Dahiya, Shahana Majid
Prostate carcinogenesis involves alterations in several signaling pathways, the most prominent being the PI3K/AKT pathway. This pathway is constitutively active and drives prostate cancer (PCa) progression to advanced metastatic disease. PTEN, a critical tumor and metastasis suppressor gene negatively regulates cell survival, proliferation, migration and angiogenesis via the PI3K/Akt pathway. PTEN is mutated, downregulated/dysfunctional in many cancers and its dysregulation correlates with poor prognosis in PCa...
October 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27509989/roles-of-microrna21-and-microrna29a-in-regulating-cell-adhesion-related-genes-in-bone-metastasis-secondary-to-prostate-cancer
#14
Maisarah Mohamad, Norhazlina Abdul Wahab, Rosna Yunus, Nor Azian Abdul Murad, Zulkifli Md Zainuddin, Murali Sundaram, Norfilza Mohd Mokhtar
BACKGROUND: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be downregulated in clinical samples, most likely due to the posttranscriptional modification by microRNAs. Targeted genes would be upregulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27231600/lensfree-diffractive-tomography-for-the-imaging-of-3d-cell-cultures
#15
F Momey, A Berdeu, T Bordy, J-M Dinten, F Kermarrec Marcel, N Picollet-D'hahan, X Gidrol, C Allier
New microscopes are needed to help realize the full potential of 3D organoid culture studies. In order to image large volumes of 3D organoid cultures while preserving the ability to catch every single cell, we propose a new imaging platform based on lensfree microscopy. We have built a lensfree diffractive tomography setup performing multi-angle acquisitions of 3D organoid culture embedded in Matrigel and developed a dedicated 3D holographic reconstruction algorithm based on the Fourier diffraction theorem...
March 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27060259/oxidative-phosphorylation-and-mitochondrial-function-differ-between-human-prostate-tissue-and-cultured-cells
#16
Bernd Schöpf, Georg Schäfer, Anja Weber, Heribert Talasz, Iris E Eder, Helmut Klocker, Erich Gnaiger
Altered mitochondrial metabolism plays a pivotal role in the development and progression of various diseases, including cancer. Cell lines are frequently used as models to study mitochondrial (dys)function, but little is known about their mitochondrial respiration and metabolic properties in comparison to the primary tissue of origin. We have developed a method for assessment of oxidative phosphorylation in prostate tissue samples of only 2 mg wet weight using high-resolution respirometry. Reliable protocols were established to investigate the respiratory activity of different segments of the mitochondrial electron transfer system (ETS) in mechanically permeabilized tissue biopsies...
June 2016: FEBS Journal
https://www.readbyqxmd.com/read/25756394/tgf%C3%AE-induced-phosphorylation-of-par6-promotes-migration-and-invasion-in-prostate-cancer-cells
#17
Y Mu, G Zang, U Engström, C Busch, M Landström
BACKGROUND: The Par complex - comprising partition-defective 6 (Par6), Par3, and atypical protein kinase C (aPKC) - is crucial for cell polarisation, the loss of which contributes to cancer progression. Transforming growth factor β (TGFβ)-induced phosphorylation of Par6 on the conserved serine 345 is implicated in epithelial-to-mesenchymal transition (EMT) in breast cancer. Here we investigated the importance of phosphorylated Par6 in prostate cancer. METHODS: We generated a p-Par6(345)-specific antibody and verified its specificity in vitro...
March 31, 2015: British Journal of Cancer
https://www.readbyqxmd.com/read/25446255/vip-induces-nf-%C3%AE%C2%BAb1-nuclear-localisation-through-different-signalling-pathways-in-human-tumour-and-non-tumour-prostate-cells
#18
Ana B Fernández-Martínez, María J Carmena, Ana M Bajo, Eva Vacas, Manuel Sánchez-Chapado, Juan C Prieto
The nuclear factor κB (NF-κB) is a powerful activator of angiogenesis, invasion and metastasis. Transactivation and nuclear localisation of NF-κB is an index of recurrence in prostate cancer. Vasoactive intestinal peptide (VIP) exerts similar effects in prostate cancer models involving increased expression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) which are related to NF-κB transactivation. Here we studied differential mechanisms of VIP-induced NF-κB transactivation in non-tumour RWPE-1 and tumour LNCaP and PC3 human prostate epithelial cells...
February 2015: Cellular Signalling
https://www.readbyqxmd.com/read/25410184/cystine-dimethyl-ester-induces-apoptosis-through-regulation-of-pkc-%C3%AE-and-pkc-%C3%AE%C2%B5-in-prostate-cancer-cells
#19
Nilgun Gurbuz, Margaret A Park, Paul Dent, Asim B Abdel Mageed, Suresh C Sikka, Asli Baykal
Protein kinase C-δ (PKC-δ) and PKC-ε are reported to be effective in cancer prevention via S-thiolation-mediated mechanisms. This may be through stimulation of the pro-apoptotic, tumor-suppressive isozyme PKC-δ and/or inactivation of the growth stimulatory, oncogenic isozyme PKC-ε. We investigated oxidative regulatory responses of PKC-δ and PKC-ε to cystine dimethyl ester (CDME), a metabolic precursor of cystine, which, by inducing release of cellular cystine stimulates apoptosis in different prostate cancer cells, PC3 and LNCaP, compared to normal RWPE1 cells...
2015: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/25237656/a-milk-protein-casein-as-a-proliferation-promoting-factor-in-prostate-cancer-cells
#20
Sung-Woo Park, Joo-Young Kim, You-Sun Kim, Sang Jin Lee, Sang Don Lee, Moon Kee Chung
PURPOSE: Despite most epidemiologic studies reporting that an increase in milk intake affects the growth of prostate cancer, the results of experimental studies are not consistent. In this study, we investigated the proliferation of prostate cancer cells treated with casein, the main protein in milk. MATERIALS AND METHODS: Prostate cancer cells (LNCaP and PC3), lung cancer cells (A459), stomach cancer cells (SNU484), breast cancer cells (MCF7), immortalized human embryonic kidney cells (HEK293), and immortalized normal prostate cells (RWPE1) were treated with either 0...
August 2014: World Journal of Men's Health
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