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https://www.readbyqxmd.com/read/28532481/identification-of-endonuclease-domain-containing-1-as-a-novel-tumor-suppressor-in-prostate-cancer
#1
Jianguang Qiu, Shubin Peng, Jie Si-Tu, Cheng Hu, Wentao Huang, Yunhua Mao, Wenhan Qiu, Ke Li, Dejuan Wang
BACKGROUND: Endonuclease domain containing 1 (ENDOD1) is implicated in tumorigenesis and aggressiveness of multiple tumors. In this study, we aimed to investigate the role of ENDOD1 in prostate cancer (PCa). METHODS: Immunohistochemistry were performed in 30 cases of benign prostatic hyperplasia (BPH) and 50 cases of PCa to identify its association with clinicopathological characteristics. Real-time PCR and western blot were used to detect ENDOD1 mRNA and protein expression in normal prostatic epithelial and PCa cell lines...
May 22, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28481876/oncogenic-activity-of-amplified-miniature-chromosome-maintenance-8-in-human-malignancies
#2
D-M He, B-G Ren, S Liu, L-Z Tan, K Cieply, G Tseng, Y P Yu, J-H Luo
Miniature chromosome maintenance (MCM) proteins play critical roles in DNA replication licensing, initiation and elongation. MCM8, one of the MCM proteins playing a critical role in DNA repairing and recombination, was found to have overexpression and increased DNA copy number in a variety of human malignancies. The gain of MCM8 is associated with aggressive clinical features of several human cancers. Increased expression of MCM8 in prostate cancer is associated with cancer recurrence. Forced expression of MCM8 in RWPE1 cells, the immortalized but non-transformed prostate epithelial cell line, exhibited fast cell growth and transformation, while knock down of MCM8 in PC3, DU145 and LNCaP cells induced cell growth arrest, and decreased tumour volumes and mortality of severe combined immunodeficiency mice xenografted with PC3 and DU145 cells...
June 22, 2017: Oncogene
https://www.readbyqxmd.com/read/28440481/the-specific-killing-effect-of-matrine-on-castration-resistant-prostate-cancer-cells-by-targeting-the-akt-foxo3a-signaling-pathway
#3
Shoumin Bai, Ting Chen, Xiaoli Yu, Ming Luo, Xianju Chen, Chunhao Lin, Yiming Lai, Hai Huang
Matrine, a Sophora alkaloid, exhibits antiproliferative and anti-carcinogenic activities through several mechanisms. In a previous study, we found that matrine could effectively inhibit the proliferation of castration-resistant prostate cancer (CRPC). In the present study, the effect of matrine and LY294002 on the expression of the Akt/FoxO3a signaling pathway was examined by western blot analyses and RT-PCR. We discovered that matrine significantly inhibited the proliferation of both prostate cancer cell line PC-3 and prostate epithelial cell line RWPE1, induced apoptosis and induced cell cycle arrest...
May 2017: Oncology Reports
https://www.readbyqxmd.com/read/28408625/ets-e26-transformation-specific-up-regulation-of-the-transcriptional-co-activator-taz-promotes-cell-migration-and-metastasis-in-prostate-cancer
#4
Chen-Ying Liu, Tong Yu, Yuji Huang, Long Cui, Wanjin Hong
Prostate cancer is a very common malignant disease and a leading cause of death for men in the Western world. Tumorigenesis and progression of prostate cancer involves multiple signaling pathways, including the Hippo pathway. Yes-associated protein (YAP) is the downstream transcriptional co-activator of the Hippo pathway, is overexpressed in prostate cancer, and plays a vital role in the tumorigenesis and progression of prostate cancer. However, the role of the YAP paralog and another downstream effector of the Hippo pathway, transcriptional co-activator with PDZ-binding motif (TAZ), in prostate cancer has not been fully elucidated...
June 2, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28385910/metformin-reduces-prostate-tumor-growth-in-a-diet-dependent-manner-by-modulating-multiple-signaling-pathways
#5
Andre Sarmento-Cabral, Fernando L-Lopez, Manuel D Gahete, Justo P Castano, Raul M Luque
Prostate-cancer is strongly influenced by obesity, wherein metformin could represent a promising treatment; however, the endocrine-metabolic/cellular/molecular-mechanisms underlying these associations and effects are still unclear. To determine the beneficial anti-tumoral effects of metformin on prostate-cancer progression/aggressiveness and the relative contribution of high-fat diet (HFD; independently of obesity), we used HFD-fed immuno-suppressed mice inoculated with PC3 cells (which exhibited partial resistance to diet-induced obesity) compared with low-fat diet (LFD)-fed control-mice...
April 6, 2017: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28374086/notch-4-silencing-inhibits-prostate-cancer-growth-and-emt-via-the-nf-%C3%AE%C2%BAb-pathway
#6
Jianwei Zhang, Youlin Kuang, Yan Wang, Quanquan Xu, Qinghua Ren
Epithelial-mesenchymal transition (EMT) is implicated in the metastasis of human prostate cancer (PCa). Notch signaling has been established as a regulator of EMT. Notch-4 has emerged as a mammary proto-oncogene and a target in several cancers. However, the role and the mechanism of action of Notch-4 in PCa are still unclear. In the present study, we first observed a marked increase in Notch-4 expression in the PCa cell lines DU145, PC3 and LnCAP compared with the non-malignant prostate epithelial cell line RWPE1...
June 2017: Apoptosis: An International Journal on Programmed Cell Death
https://www.readbyqxmd.com/read/27634912/oncogenic-microrna-4534-regulates-pten-pathway-in-prostate-cancer
#7
Hannah Nip, Altaf A Dar, Sharanjot Saini, Melissa Colden, Shahryari Varahram, Harshika Chowdhary, Soichiro Yamamura, Yozo Mitsui, Yuichiro Tanaka, Taku Kato, Yutaka Hashimoto, Marisa Shiina, Priyanka Kulkarni, Pritha Dasgupta, Mitsuho Imai-Sumida, Z Laura Tabatabai, Kirsten Greene, Guoren Deng, Rajvir Dahiya, Shahana Majid
Prostate carcinogenesis involves alterations in several signaling pathways, the most prominent being the PI3K/AKT pathway. This pathway is constitutively active and drives prostate cancer (PCa) progression to advanced metastatic disease. PTEN, a critical tumor and metastasis suppressor gene negatively regulates cell survival, proliferation, migration and angiogenesis via the PI3K/Akt pathway. PTEN is mutated, downregulated/dysfunctional in many cancers and its dysregulation correlates with poor prognosis in PCa...
October 18, 2016: Oncotarget
https://www.readbyqxmd.com/read/27509989/roles-of-microrna21-and-microrna29a-in-regulating-cell-adhesion-related-genes-in-bone-metastasis-secondary-to-prostate-cancer
#8
Maisarah Mohamad, Norhazlina Abdul Wahab, Rosna Yunus, Nor Azian Abdul Murad, Zulkifli Md Zainuddin, Murali Sundaram, Norfilza Mohd Mokhtar
BACKGROUND: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be downregulated in clinical samples, most likely due to the posttranscriptional modification by microRNAs. Targeted genes would be upregulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors...
2016: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/27231600/lensfree-diffractive-tomography-for-the-imaging-of-3d-cell-cultures
#9
F Momey, A Berdeu, T Bordy, J-M Dinten, F Kermarrec Marcel, N Picollet-D'hahan, X Gidrol, C Allier
New microscopes are needed to help realize the full potential of 3D organoid culture studies. In order to image large volumes of 3D organoid cultures while preserving the ability to catch every single cell, we propose a new imaging platform based on lensfree microscopy. We have built a lensfree diffractive tomography setup performing multi-angle acquisitions of 3D organoid culture embedded in Matrigel and developed a dedicated 3D holographic reconstruction algorithm based on the Fourier diffraction theorem...
March 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27060259/oxidative-phosphorylation-and-mitochondrial-function-differ-between-human-prostate-tissue-and-cultured-cells
#10
Bernd Schöpf, Georg Schäfer, Anja Weber, Heribert Talasz, Iris E Eder, Helmut Klocker, Erich Gnaiger
Altered mitochondrial metabolism plays a pivotal role in the development and progression of various diseases, including cancer. Cell lines are frequently used as models to study mitochondrial (dys)function, but little is known about their mitochondrial respiration and metabolic properties in comparison to the primary tissue of origin. We have developed a method for assessment of oxidative phosphorylation in prostate tissue samples of only 2 mg wet weight using high-resolution respirometry. Reliable protocols were established to investigate the respiratory activity of different segments of the mitochondrial electron transfer system (ETS) in mechanically permeabilized tissue biopsies...
June 2016: FEBS Journal
https://www.readbyqxmd.com/read/25756394/tgf%C3%AE-induced-phosphorylation-of-par6-promotes-migration-and-invasion-in-prostate-cancer-cells
#11
Y Mu, G Zang, U Engström, C Busch, M Landström
BACKGROUND: The Par complex - comprising partition-defective 6 (Par6), Par3, and atypical protein kinase C (aPKC) - is crucial for cell polarisation, the loss of which contributes to cancer progression. Transforming growth factor β (TGFβ)-induced phosphorylation of Par6 on the conserved serine 345 is implicated in epithelial-to-mesenchymal transition (EMT) in breast cancer. Here we investigated the importance of phosphorylated Par6 in prostate cancer. METHODS: We generated a p-Par6(345)-specific antibody and verified its specificity in vitro...
March 31, 2015: British Journal of Cancer
https://www.readbyqxmd.com/read/25446255/vip-induces-nf-%C3%AE%C2%BAb1-nuclear-localisation-through-different-signalling-pathways-in-human-tumour-and-non-tumour-prostate-cells
#12
Ana B Fernández-Martínez, María J Carmena, Ana M Bajo, Eva Vacas, Manuel Sánchez-Chapado, Juan C Prieto
The nuclear factor κB (NF-κB) is a powerful activator of angiogenesis, invasion and metastasis. Transactivation and nuclear localisation of NF-κB is an index of recurrence in prostate cancer. Vasoactive intestinal peptide (VIP) exerts similar effects in prostate cancer models involving increased expression of vascular endothelial growth factor (VEGF) and cyclooxygenase-2 (COX-2) which are related to NF-κB transactivation. Here we studied differential mechanisms of VIP-induced NF-κB transactivation in non-tumour RWPE-1 and tumour LNCaP and PC3 human prostate epithelial cells...
February 2015: Cellular Signalling
https://www.readbyqxmd.com/read/25410184/cystine-dimethyl-ester-induces-apoptosis-through-regulation-of-pkc-%C3%AE-and-pkc-%C3%AE%C2%B5-in-prostate-cancer-cells
#13
Nilgun Gurbuz, Margaret A Park, Paul Dent, Asim B Abdel Mageed, Suresh C Sikka, Asli Baykal
Protein kinase C-δ (PKC-δ) and PKC-ε are reported to be effective in cancer prevention via S-thiolation-mediated mechanisms. This may be through stimulation of the pro-apoptotic, tumor-suppressive isozyme PKC-δ and/or inactivation of the growth stimulatory, oncogenic isozyme PKC-ε. We investigated oxidative regulatory responses of PKC-δ and PKC-ε to cystine dimethyl ester (CDME), a metabolic precursor of cystine, which, by inducing release of cellular cystine stimulates apoptosis in different prostate cancer cells, PC3 and LNCaP, compared to normal RWPE1 cells...
2015: Anti-cancer Agents in Medicinal Chemistry
https://www.readbyqxmd.com/read/25237656/a-milk-protein-casein-as-a-proliferation-promoting-factor-in-prostate-cancer-cells
#14
Sung-Woo Park, Joo-Young Kim, You-Sun Kim, Sang Jin Lee, Sang Don Lee, Moon Kee Chung
PURPOSE: Despite most epidemiologic studies reporting that an increase in milk intake affects the growth of prostate cancer, the results of experimental studies are not consistent. In this study, we investigated the proliferation of prostate cancer cells treated with casein, the main protein in milk. MATERIALS AND METHODS: Prostate cancer cells (LNCaP and PC3), lung cancer cells (A459), stomach cancer cells (SNU484), breast cancer cells (MCF7), immortalized human embryonic kidney cells (HEK293), and immortalized normal prostate cells (RWPE1) were treated with either 0...
August 2014: World Journal of Men's Health
https://www.readbyqxmd.com/read/25227780/dna-fragmentation-caspase-3-and-prostate-specific-antigen-genes-expression-induced-by-arsenic-cadmium-and-chromium-on-nontumorigenic-human-prostate-cells
#15
Hend M Abo El-Atta, Amal A El-Bakary, Afaf M Attia, Ahmed Lotfy, Shery S Khater, Ayman Z Elsamanoudy, Hussein Abdelaziz Abdalla
Prostate cancer is one of the most common cancers and the second cause of cancer-related deaths among men. Metals are recognized as chemical carcinogens where chronic exposures to such metals are implicated in the development of cancer, including prostate cancer. This in vitro study demonstrates the relative death sensitivity of prostatic (RWPE-1) cells to arsenic (As), cadmium (Cd), and chromium (Cr) as environmental pollutants through its apoptotic effects and the effect of these chemicals on prostate-specific antigen (PSA) gene expression as a marker for their carcinogecity...
December 2014: Biological Trace Element Research
https://www.readbyqxmd.com/read/25194810/ubiquitin-c-terminal-hydrolase-l3-regulates-emt-process-and-cancer-metastasis-in-prostate-cell-lines
#16
Hyun Min Song, Jae Eun Lee, Jung Hwa Kim
Ubiquitin C-terminal hydrolase-L3 (UCH-L3) is among the deubiquitinating enzymes (DUBs) that cleave ubiquitin (Ub) from Ub precursors or protein substrates. Many DUBs have been shown to participate in cancer progression in various tissues. However, the mechanism and role of UCH-L3 in carcinogenesis has largely been unknown until recently. Here we investigated the implication of UCH-L3 in prostate cancer progression. Interestingly, UCH-L3 is upregulated in normal or non-metastatic prostate cancer cells and is downregulated in metastatic prostate cancer cell lines...
September 26, 2014: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/24886089/dtx3l-and-artd9-inhibit-irf1-expression-and-mediate-in-cooperation-with-artd8-survival-and-proliferation-of-metastatic-prostate-cancer-cells
#17
Samia B Bachmann, Sandra C Frommel, Rosalba Camicia, Hans C Winkler, Raffaella Santoro, Paul O Hassa
BACKGROUND: Prostate cancer (PCa) is one of the leading causes of cancer-related mortality and morbidity in the aging male population and represents the most frequently diagnosed malignancy in men around the world. The Deltex (DTX)-3-like E3 ubiquitin ligase (DTX3L), also known as B-lymphoma and BAL-associated protein (BBAP), was originally identified as a binding partner of the diphtheria-toxin-like macrodomain containing ADP-ribosyltransferase-9 (ARTD9), also known as BAL1 and PARP9...
May 27, 2014: Molecular Cancer
https://www.readbyqxmd.com/read/24753248/determination-of-carbohydrate-structure-recognized-by-prostate-specific-f77-monoclonal-antibody-through-expression-analysis-of-glycosyltransferase-genes
#18
Motohiro Nonaka, Michiko N Fukuda, Chao Gao, Zhen Li, Hongtao Zhang, Mark I Greene, Donna M Peehl, Ten Feizi, Minoru Fukuda
This study reports the determination of the carbohydrate epitope of monoclonal antibody F77 previously raised against human prostate cancer PC-3 cells (Zhang, G., Zhang, H., Wang, Q., Lal, P., Carroll, A. M., de la Llera-Moya, M., Xu, X., and Greene, M. I. (2010) Proc. Natl. Acad. Sci. U. S. A. 107, 732-737). We performed a series of co-transfections using mammalian expression vectors encoding specific glycosyltransferases. We thereby identified branching enzymes and FUT1 (required for Fucα1→2Gal linkage) as being essential for F77 antigen formation...
June 6, 2014: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/24666255/formononetin-induces-apoptosis-in-pc-3-prostate-cancer-cells-through-enhancing-the-bax-bcl-2-ratios-and-regulating-the-p38-akt-pathway
#19
Xing Zhang, Lingyun Bi, Yu Ye, Jian Chen
Formononetin (FN), a bioactive component extracted from the red clover (Trifolium pratense L.), has been long used for treating carcinomas in China. In the present study, we aim to investigate the potential therapeutical effects of FN on cell line of prostatic adenocarcinoma (PC-3) and human prostate epithelial cells (RWPE1). These findings indicated that FN significantly inhibited the cell growth of PC-3 in a dose-dependent manner, but no such effect was observed in RWPE1 cells. The apoptotic counts were effectively increased following the treatments as shown in flow cytometry...
2014: Nutrition and Cancer
https://www.readbyqxmd.com/read/24583925/tr4-nuclear-receptor-functions-as-a-tumor-suppressor-for-prostate-tumorigenesis-via-modulation-of-dna-damage-repair-system
#20
Shin-Jen Lin, Soo Ok Lee, Yi-Fen Lee, Hiroshi Miyamoto, Dong-Rong Yang, Gonghui Li, Chawnshang Chang
Testicular nuclear receptor 4 (TR4), a member of the nuclear receptor superfamily, plays important roles in metabolism, fertility and aging. The linkage of TR4 functions in cancer progression, however, remains unclear. Using three different mouse models, we found TR4 could prevent or delay prostate cancer (PCa)/prostatic intraepithelial neoplasia development. Knocking down TR4 in human RWPE1 and mouse mPrE normal prostate cells promoted tumorigenesis under carcinogen challenge, suggesting TR4 may play a suppressor role in PCa initiation...
June 2014: Carcinogenesis
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