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Francois Derimay, Geraud Souteyrand, Pascal Motreff, Gilles Rioufol, Gerard Finet
AIMS: The rePOT (proximal optimizing technique) sequence proved significantly more effective than final kissing balloon (FKB) with two drug-eluting stents (DES) in a bench test. We sought to experimentally validate efficacy in a large range of latest-generation DESs. METHODS AND RESULTS: On left main fractal coronary bifurcation bench models, 5 samples of each of the 6 main latest-generation DESs (Coroflex Isar™, Orsiro™, Promus Premier™, Resolute Integrity™, Ultimaster™, Xience Xpedition™) were implanted on rePOT (initial POT, side-branch inflation, final POT)...
May 9, 2017: EuroIntervention
Henry Y Chen, Khalid Al-Saadon, Yves Louvard, Ghassan S Kassab
BACKGROUND: In-stent restenosis (ISR) and stent thrombosis remain clinically significant problems for bifurcations. Although the role of wall shear stress (WSS) has been well investigated, the role of circumferential wall stresses (CWS) has not been well studied in provisional stenting with and without final kissing balloon (FKB). METHODS: We hypothesized that the perturbation of CWS at the SB in provisional stenting and balloon dilatation is an important factor in addition to WSS, and hence may affect restenosis rates (i...
April 27, 2017: Journal of Applied Physiology
Alison McAfee, Troy F Collins, Lufiani L Madilao, Leonard J Foster
Hygienic behaviour (HB) is a social immunity trait in honey bees (Apis mellifera L.) whereby workers detect, uncap and remove unhealthy brood, improving disease resistance in the colony. This is clearly economically valuable; however, the molecular mechanism behind it is not well understood. The freeze-killed brood (FKB) assay is the conventional method of HB selection, so we compared odour profiles of FKB and live brood to find candidate HB-inducing odours. Surprisingly, we found that significantly more brood pheromone (β-ocimene) was released from FKB...
April 7, 2017: Scientific Reports
Chia-Ting Chang, You-Cheng Hseu, Varadharajan Thiyagarajan, Kai-Yuan Lin, Tzong-Der Way, Mallikarjuna Korivi, Jiuun-Wang Liao, Hsin-Ling Yang
Flavokawain B (FKB), a naturally occurring chalcone in kava extracts, has been reported to possess anticancer activity. However, the effect of FKB on gastric cancer remains unclear. We examined the in vitro and in vivo anticancer activity and autophagy involvement of FKB and determined the underlying molecular mechanisms. FKB is potently cytotoxic to human gastric cancer cells (AGS/NCI-N87/KATO-III/TSGH9201) and mildly toxic towards normal (Hs738) cells and primary mouse hepatocytes. FKB-induced AGS cell death was characterized by autophagy, not apoptosis, as evidenced by increased LC3-II accumulation, GFP-LC3 puncta and acidic vesicular organelles (AVOs) formation, without resulting procaspase-3/PARP cleavage...
April 3, 2017: Archives of Toxicology
Lihua Dong, Yongjun Liu
Chorismatase is an important enzyme involved in Shikimate pathway, which catalyzes the conversion of chorismate into pyruvate and (dihydro)-benzoic acid derivatives. According to the outcomes of catalytic reactions, chorismatases can be divided into three subfamilies: CH-Fkbo, CH-Hyg5 and CH-XanB2. Recently, the crystal structures of CH-Fkbo and CH-Hyg5 from Streptomyces hygroscopicus have been successfully obtained, allowing us to perform QM/MM calculations to explore the reaction details. Our calculation results support the proposal that CH-Fkbo and CH-Hyg5 employ different catalytic mechanisms and gave the mechanistic details...
March 6, 2017: Proteins
Benjamin Alleva, Nathan Balukoff, Amy Peiper, Sarit Smolikove
In meiotic prophase I, homologous chromosome pairing is promoted through chromosome movement mediated by nuclear envelope proteins, microtubules, and dynein. After proper homologue pairing has been established, the synaptonemal complex (SC) assembles along the paired homologues, stabilizing their interaction and allowing for crossing over to occur. Previous studies have shown that perturbing chromosome movement leads to pairing defects and SC polycomplex formation. We show that FKB-6 plays a role in SC assembly and is required for timely pairing and proper double-strand break repair kinetics...
February 2017: Journal of Cell Biology
Jung-Min Ahn, Pil Hyung Lee, Duk-Woo Park, Soo-Jin Kang, Seung-Whan Lee, Young-Hak Kim, Cheol Whan Lee, Seong-Wook Park, Seung-Jung Park
The role of final kissing balloon (FKB) inflation after simple crossover stenting in unprotected left main (LM) bifurcation stenosis remains unknown. From the Asan Medical Center-Left Main Revascularization (ASAN-MAIN) registry, 413 patients with LM bifurcation stenosis treated by simple crossover stenting with a drug-eluting stent were identified. After simple crossover stenting, FKB inflation was performed in 95 patients (FKB group) and 318 patients finished the procedure without FKB (no-FKB group). The primary end points of the 2-year incidence of major adverse cardiac events (death, myocardial infarction, and left main target lesion revascularization [LM-TLR]) were similar between the FKB and no-FKB groups (12...
February 15, 2017: American Journal of Cardiology
Swee Keong Yeap, Nadiah Abu, Nadeem Akthar, Wan Yong Ho, Huynh Ky, Sheau Wei Tan, Noorjahan Banu Alitheen, Tunku Kamarul
Flavokawain B (FKB) is known to possess promising anticancer abilities. This is demonstrated in various cancer cell lines including HeLa cells. Cervical cancer is among the most widely diagnosed cancer among women today. Though FKB has been shown to be effective in treating cancer cells, the exact molecular mechanism is still unknown. This study is aimed at understanding the effects of FKB on HeLa cells using a microarray-based mRNA expression profiling and proteome profiling of stress-related proteins. The results of this study suggest that FKB induced cell death through p21-mediated cell cycle arrest and activation of p38...
July 24, 2016: Integrative Cancer Therapies
Christina Dimopoulou, Sarah M Leistner, Marcus Ising, Harald J Schneider, Jochen Schopohl, Sandra Rutz, Robert Kosilek, Richard Frohner, Gunter K Stalla, Caroline Sievers
OBJECTIVE: Diagnosis of acromegaly is delayed up to 10 years after disease onset despite obvious external/objective changes such as bone and soft-tissue deformities. We hypothesized that a lack of subjective perception of the disease state, possibly mediated by psychiatric or cognitive alterations, might contribute to the delayed initiation of a diagnostic work-up. DESIGN: Cross-sectional study. METHODS: We investigated perceived body image by standardized questionnaires (FKB-20: Fragebogen zum Körperbild; FBeK: Fragebogen zur Beurteilung des eigenen Körpers) in 81 acromegalic patients and contrasted them to a) a clinical control group of 60 patients with non-functioning pituitary adenomas (NFPA) who lack severe facial and physical alterations and b) healthy controls...
July 25, 2016: Neuroendocrinology
María Ordóñez-Robles, Antonio Rodríguez-García, Juan F Martín
Tacrolimus (FK506) is a 23-membered macrolide immunosuppressant used in current clinics. Understanding how the tacrolimus biosynthetic gene cluster is regulated is important to increase its industrial production. Here, we analysed the effect of the disruption of fkbN (encoding a LAL-type positive transcriptional regulator) on the whole transcriptome of the tacrolimus producer Streptomyces tsukubaensis using microarray technology. Transcription of fkbN in the wild type strain increases from 70 h of cultivation reaching a maximum at 89 h, prior to the onset of tacrolimus biosynthesis...
September 2016: Applied Microbiology and Biotechnology
Robert J Gil, Jacek Bil, Ricardo A Costa, Katarzyna E Gil, Dobrin Vassiliev
The aim of this study was to analyze the difference in neointima pattern assessed by intravascular ultrasound (IVUS) between two dedicated bifurcation stents, BiOSS® Expert and BiOSS® LIM at 12-month follow-up. This manuscript reports IVUS findings obtained from the analysis of patients enrolled into first-in-man registries initially assessing the BiOSS Expert® (paclitaxel) and BiOSS LIM® (sirolimus) stents. Quantitative angiographic analysis was performed pre, post-stenting, and at follow-up. IVUS examination was performed at 12 months...
September 2016: International Journal of Cardiovascular Imaging
Nadiah Abu, M Nadeem Akhtar, Swee Keong Yeap, Kian Lam Lim, Wan Yong Ho, Mohd Puad Abdullah, Chai Ling Ho, Abdul Rahman Omar, Jamil Ismail, Noorjahan Banu Alitheen
BACKGROUND: The kava-kava plant (Piper methysticum) is traditionally consumed by the pacific islanders and has been linked to be involved in several biological activities. Flavokawain B is a unique chalcone, which can be found in the roots of the kava-kava plant. In this study, the operational mechanism of the anti-cancer activity of a synthetic Flavokawain B (FKB) on two breast cancer cell lines, MCF-7 and MDA-MB231 was investigated. METHOD: Several in vitro assays were attempted such as MTT, flow cytometry of cell cycle analysis, annexin V analysis, and JC-1 analysis to detect apoptosis...
2016: BMC Complementary and Alternative Medicine
Keanu D Pinner, Christina T K Wales, Rachel A Gristock, Hoa T Vo, Nadine So, Aaron T Jacobs
Context Flavokawains are secondary metabolites from the kava plant (Piper methysticum Forst. f., Piperaceae) that have anticancer properties and demonstrated oral efficacy in murine cancer models. However, flavokawains also have suspected roles in rare cases of kava-induced hepatotoxicity. Objective To compare the toxicity flavokawains A and B (FKA, FKB) and monitor the resulting transcriptional responses and cellular adaptation in the human hepatocyte cell line, HepG2. Materials and methods HepG2 were treated with 2-100 μM FKA or FKB for 24-48 h...
September 2016: Pharmaceutical Biology
Martina Rudgalvyte, Juhani Peltonen, Merja Lakso, Richard Nass, Garry Wong
Manganese (Mn) is an essential nutrient; nonetheless, excessive amounts can accumulate in brain tissues causing manganism, a severe neurological condition. Previous studies have suggested oxidative stress, mitochondria dysfunction, and impaired metabolism pathways as routes for Mn toxicity. Here, we used the nematode Caenorhabditis elegans to analyze gene expression changes after acute Mn exposure using RNA-Seq. L1 stage animals were exposed to 50 mM MnCl2 for 30 min and analyzed at L4. We identified 746 up- and 1828 downregulated genes (FDR corrected p < 0...
February 2016: Journal of Biochemical and Molecular Toxicology
Cheol Woong Yu, Jeong Hoon Yang, Young Bin Song, Joo-Yong Hahn, Seung-Hyuk Choi, Jin-Ho Choi, Hyun Jong Lee, Ju Hyeon Oh, Bon-Kwon Koo, Seung Woon Rha, Jin-Ok Jeong, Myung-Ho Jeong, Jung Han Yoon, Yangsoo Jang, Seung-Jea Tahk, Hyo-Soo Kim, Hyeon-Cheol Gwon
OBJECTIVES: This study investigated the impact of final kissing ballooning (FKB) after main vessel (MV) stenting on outcomes in patients with coronary bifurcation lesions after application of the 1-stent technique. BACKGROUND: Although FKB has been established as the standard method for bifurcation lesions treated with a 2-stent strategy, its efficacy in a 1-stent approach is highly controversial. METHODS: This study enrolled 1,901 patients with a bifurcation lesion with a side branch diameter ≥2...
August 24, 2015: JACC. Cardiovascular Interventions
Katharina Zenger, Sara Agnolet, Bernd Schneider, Birgit Kraus
The in vitro metabolism of flavokawains A, B, and C (FKA, FKB, FKC), methoxylated chalcones from Piper methysticum, was examined using human liver microsomes. Phase I metabolism and phase II metabolism (glucuronidation) as well as combined phase I+II metabolism were studied. For identification and structure elucidation of microsomal metabolites, LC-HRESIMS and NMR techniques were applied. Major phase I metabolites were generated by demethylation in position C-4 or C-4' and hydroxylation predominantly in position C-4, yielding FKC as phase I metabolite of FKA and FKB, helichrysetin as metabolite of FKA and FKC, and cardamonin as metabolite of FKC...
July 22, 2015: Journal of Agricultural and Food Chemistry
Sefik Tagay, Marion Lindner, Hans-Christoph Friederich, Ellen Schlottbohm
The aim of this study was the validation of a short disease-specific questionnaire (ELI, Essen Quality of Life Index for Eating Disorders) to measure the health-related quality of life in patients with eating disorders. A total of 182 currently ill and former eating disordered patients and 87 healthy controls completed the ELI questionnaire as well as other reliable and valid instruments (EDQOL, SF-12, EDI-2, FKB-20, SEED, BSI, IIP-D and SOC-13). In addition, 46 eating disorder patients completed the same questionnaires at the end of therapy...
September 2015: Psychotherapie, Psychosomatik, Medizinische Psychologie
Nadiah Abu, Nurul Elyani Mohamed, Swee Keong Yeap, Kian Lam Lim, M Nadeem Akhtar, Aimi Jamil Zulfadli, Beh Boon Kee, Mohd Puad Abdullah, Abdul Rahman Omar, Noorjahan Banu Alitheen
Flavokawain B (FKB) is a naturally occurring chalcone that can be isolated through the root extracts of the kava-kava plant (Piper methysticum). It can also be synthesized chemically to increase the yield. This compound is a promising candidate as a biological agent, as it is reported to be involved in a wide range of biological activities. Furthermore, FKB was reported to have antitumorigenic effects in several cancer cell lines in vitro. However, the in vivo antitumor effects of FKB have not been reported on yet...
2015: Drug Design, Development and Therapy
Robert J Gil, Jacek Bil, Vladimír Džavík, Dobrin Vassilev, Adam Kern, Radoslaw Formuszewicz, Małgorzata Zalewska-Adamiec, Sławomir Dobrzycki
BACKGROUND: Results of regular drug-eluting stents (rDESs) in bifurcation treatment are not optimal. The aim of the Polish Bifurcation Optimal Stenting I (POLBOS I) trial was to compare bifurcation treatment with any rDES vs the dedicated bifurcation paclitaxel-eluting stent BiOSS Expert (Balton, Poland). The second aim was to study the effect of final kissing balloon (FKB) inflation on clinical outcomes. METHODS: Between October 2010 and January 2013 patients with stable coronary artery disease or non-ST-elevation acute coronary syndrome were assigned 1:1 to 1 of 2 treatment strategies: BiOSS Expert stent or rDES implantation...
May 2015: Canadian Journal of Cardiology
Zhan Gao, Bo Xu, Yue-Jin Yang, Shu-Bin Qiao, Yong-Jian Wu, Tao Chen, Liang Xu, Jin-Qing Yuan, Jue Chen, Xue-Wen Qin, Min Yao, Hai-Bo Liu, Shi-Jie You, Ye-Lin Zhao, Hong-Bing Yan, Ji-Lin Chen, Run-Lin Gao
BACKGROUND: Whether final kissing balloon (FKB) dilatation after one-stent implantation at left-main (LM) bifurcation site remains unclear. Therefore, this large sample and long-term follow-up study comparatively assessed the impact of FKB in patients with unprotected LM disease treated with one-stent strategy. METHODS: Total 1528 consecutive patients underwent LM percutaneous coronary intervention in one center from January 2004 to December 2010 were enrolled; among them, 790 patients treated with one drug-eluting stent crossover LM to left anterior descending (LAD) with FKB (n = 230) or no FKB (n = 560) were comparatively analyzed...
March 20, 2015: Chinese Medical Journal
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