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Epcam cadherin cancer

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https://www.readbyqxmd.com/read/27417942/method-for-semi-automated-microscopy-of-filtration-enriched-circulating-tumor-cells
#1
Emma Pailler, Marianne Oulhen, Fanny Billiot, Alexandre Galland, Nathalie Auger, Vincent Faugeroux, Corinne Laplace-Builhé, Benjamin Besse, Yohann Loriot, Maud Ngo-Camus, Merouan Hemanda, Colin R Lindsay, Jean-Charles Soria, Philippe Vielh, Françoise Farace
BACKGROUND: Circulating tumor cell (CTC)-filtration methods capture high numbers of CTCs in non-small-cell lung cancer (NSCLC) and metastatic prostate cancer (mPCa) patients, and hold promise as a non-invasive technique for treatment selection and disease monitoring. However filters have drawbacks that make the automation of microscopy challenging. We report the semi-automated microscopy method we developed to analyze filtration-enriched CTCs from NSCLC and mPCa patients. METHODS: Spiked cell lines in normal blood and CTCs were enriched by ISET (isolation by size of epithelial tumor cells)...
2016: BMC Cancer
https://www.readbyqxmd.com/read/27157930/gene-expression-profiling-of-single-circulating-tumor-cells-in%C3%A2-ovarian-cancer-establishment-of-a-multi-marker-gene-panel
#2
Christina Blassl, Jan Dominik Kuhlmann, Alessandra Webers, Pauline Wimberger, Tanja Fehm, Hans Neubauer
The presence of circulating tumor cells (CTCs) in the blood of ovarian cancer patients was shown to correlate with decreased overall survival, whereby CTCs with epithelial-mesenchymal-transition (EMT) or stem-like traits are supposed to be involved in metastatic progression and recurrence. Thus, investigating the transcriptional profiles of CTCs might help to identify therapy resistant tumor cells and to overcome treatment failure. For this purpose, we established a multi-marker panel for the molecular characterization of single CTCs, detecting epithelial (EpCAM, Muc-1, CK5/7), EMT (N-cadherin, Vimentin, Snai1/2, CD117, CD146, CD49f) and stem cell (CD44, ALDH1A1, Nanog, SOX2, Notch1/4, Oct4, Lin28) associated transcripts...
August 2016: Molecular Oncology
https://www.readbyqxmd.com/read/27101285/circulating-tumor-cell-composition-in-renal-cell-carcinoma
#3
Ivonne Nel, Thomas C Gauler, Kira Bublitz, Lazaros Lazaridis, André Goergens, Bernd Giebel, Martin Schuler, Andreas-Claudius Hoffmann
PURPOSE: Due to their minimal-invasive yet potentially current character circulating tumor cells (CTC) might be useful as a "liquid biopsy" in solid tumors. However, successful application in metastatic renal cell carcinoma (mRCC) has been very limited so far. High plasticity and heterogeneity of CTC morphology challenges currently available enrichment and detection techniques with EpCAM as the usual surface marker being underrepresented in mRCC. We recently described a method that enables us to identify and characterize non-hematopoietic cells in the peripheral blood stream with varying characteristics and define CTC subgroups that distinctly associate to clinical parameters...
2016: PloS One
https://www.readbyqxmd.com/read/27021602/transcriptome-profile-of-the-early-stages-of-breast-cancer-tumoral-spheroids
#4
Rosario Pacheco-Marín, Jorge Melendez-Zajgla, Gonzalo Castillo-Rojas, Edna Mandujano-Tinoco, Alfredo Garcia-Venzor, Salvador Uribe-Carvajal, Alfredo Cabrera-Orefice, Carolina Gonzalez-Torres, Javier Gaytan-Cervantes, Irma B Mitre-Aguilar, Vilma Maldonado
Oxygen or nutrient deprivation of early stage tumoral spheroids can be used to reliably mimic the initial growth of primary and metastatic cancer cells. However, cancer cell growth during the initial stages has not been fully explored using a genome-wide approach. Thus, in the present study, we investigated the transcriptome of breast cancer cells during the initial stages of tumoral growth using RNAseq in a model of Multicellular Tumor Spheroids (MTS). Network analyses showed that a metastatic signature was enriched as several adhesion molecules were deregulated, including EPCAM, E-cadherin, integrins and syndecans, which were further supported by an increase in cell migration...
2016: Scientific Reports
https://www.readbyqxmd.com/read/26946151/identification-of-cell-surface-markers-for-detecting-breast-cancer-cells-in-ovarian-tissue
#5
Inge T A Peters, Carina G J M Hilders, Cornelis F M Sier, Alexander L Vahrmeijer, Vincent T H B M Smit, J Baptist Trimbos, Peter J K Kuppen
PURPOSE: The safety of ovarian tissue autotransplantation in oncology patients cannot be ensured, as current tumor-detection methods compromise the ovarian tissue viability. Although non-destructive methods (for instance near-infrared fluorescence imaging) can discriminate malignant from healthy tissues while leaving the examined tissues unaffected, they require specific cell-surface tumor markers. We determined which tumor markers are suitable targets for tumor-specific imaging to exclude the presence of breast cancer cells in ovarian tissue...
August 2016: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/26849897/%C3%AE-catenin-is-important-for-cancer-stem-cell-generation-and-tumorigenic-activity-in-nasopharyngeal-carcinoma
#6
Rui Jiang, Xiaoshuang Niu, Yuxiang Huang, Xiaosheng Wang
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors with poor prognosis and recurrence in South China. The hard eradication of NPC in clinic is predominantly due to cancer stem cells (CSCs). Increasing evidence revealed that the aberrant activation of Wnt/β-catenin was positively correlated with the produce of CSCs. To further investigate the effect of β-catenin on CSCs and tumorigenesis in NPC, a CNE2 cell line (pLKO.1-sh-β-catenin-CNE2) with stably suppressed expression of β-catenin was used in this study...
March 2016: Acta Biochimica et Biophysica Sinica
https://www.readbyqxmd.com/read/26790446/contribution-of-in-vitro-comparison-of-colorectal-carcinoma-cells-from-primary-and-metastatic-lesions-to-elucidation-of-mechanisms-of-tumor-progression-and-response-to-anticancer-therapy
#7
Lukáš Krbal, Veronika Hanušová, Jiří Soukup, Stanislav John, Petra Matoušková, Aleš Ryška
Colorectal cancer has been a leading cause of cancer-related morbidity and mortality. For the research and individualization of therapy, primary cell lines of the colorectal cancer appear to be still an invaluable tool. We evaluated the differences in metastatic potential between four isolated primary colon cancer cells and cells derived from their lymph node metastasis. These results were compared with correspond immortalized cells-SW480 and SW620, respectively. The ability to migrate was tested using real-time measurement in xCELLigence system...
July 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/26493939/the-role-of-epcam-in-physiology-and-pathology-of-the-epithelium
#8
REVIEW
Agnieszka Martowicz, Andreas Seeber, Gerold Untergasser
Epithelial Cell Adhesion Molecule (EpCAM) has been discovered as one of the first tumor-specific antigens overexpressed in epithelial cancer. The present review focuses on the role of EpCAM in physiology and homeostasis of epithelia. Recent research pointed to a close interaction of EpCAM with other cell-cell contact molecules like E-cadherin and claudins and an intimate crosstalk with Wnt and TGF-beta signaling in the regulation of cell growth. Moreover, EpCAM has been shown to modulate trans-epithelial migration processes of white blood cells...
April 2016: Histology and Histopathology
https://www.readbyqxmd.com/read/26457068/ideal-sphere-forming-culture-conditions-to-maintain-pluripotency-in-a-hepatocellular-carcinoma-cell-lines
#9
Seon Ok Min, Sang Woo Lee, Seon Young Bak, Kyung Sik Kim
BACKGROUND: Cancer stem cells (CSCs) constitute 1-2 % of cancer tissue and are a major cause of tumor metastasis and recurrence. The culture environment is important in maintaining CSCs in vitro. Sphere formation is one method of culturing CSCs. In this study, we identified and validated optimal culture conditions for sphere formation in hepatocellular carcinoma cells. METHODS: Huh7 and HepG2 cells were plated in three different media types and were allowed to form spheres...
2015: Cancer Cell International
https://www.readbyqxmd.com/read/26165819/hepatic-stellate-cells-promote-upregulation-of-epithelial-cell-adhesion-molecule-and-epithelial-mesenchymal-transition-in-hepatic-cancer-cells
#10
Teruya Nagahara, Hidenori Shiraha, Hiroaki Sawahara, Daisuke Uchida, Yasuto Takeuchi, Masaya Iwamuro, Junro Kataoka, Shigeru Horiguchi, Takeshi Kuwaki, Hideki Onishi, Shinichiro Nakamura, Akinobu Takaki, Kazuhiro Nouso, Kazuhide Yamamoto
Microenvironment plays an important role in epithelial-mesenchymal transition (EMT) and stemness of cells in hepatocellular carcinoma (HCC). Epithelial cell adhesion molecule (EpCAM) is known as a tumor stemness marker of HCC. To investigate the relationship between microenvironment and stemness, we performed an in vitro co-culture assay. Four HCC cell lines (HepG2, Hep3B, HuH-7 and PLC/PRF/5) were co-cultured with the TWNT-1 immortalized hepatic stellate cells (HSCs), which create a microenvironment with HCC...
September 2015: Oncology Reports
https://www.readbyqxmd.com/read/26124671/roles-of-dopamine-receptors-and-their-antagonist-thioridazine-in-hepatoma-metastasis
#11
Meiling Lu, Jinghua Li, Zaili Luo, Shuai Zhang, Shaobo Xue, Kesheng Wang, Yan Shi, Cunzhen Zhang, Haiyang Chen, Zhong Li
Tumor metastasis is the most common cause of death and poor prognosis for cancer patients. Therapeutics that prevent tumor metastasis are the key to prolonging the lifespan of cancer patients. Cancer stem cells are believed to be critical in the metastatic process. Recently, drug screening for cancer stem cells reports that antipsychotic drugs displayed potential anticancer activity. Thioridazine, one of the antipsychotic drugs for dopamine receptors (DRs), is shown to induce the differentiation of cancer stem cells in leukemic disease and breast cancer, but it is not known if this drug would affect liver cancer...
2015: OncoTargets and Therapy
https://www.readbyqxmd.com/read/26124007/bmp-2-induces-motility-and-invasiveness-by-promoting-colon-cancer-stemness-through-stat3-activation
#12
Bo Ram Kim, Sang Cheul Oh, Dae-Hee Lee, Jung Lim Kim, Suk Young Lee, Myoung Hee Kang, Sun Il Lee, Sanghee Kang, Sung Yup Joung, Byung Wook Min
Bone morphogenetic proteins (BMPs) have been involved in metastatic progression and tumorigenesis of many cancer types. However, it remains unclear how BMP-2 contributes to the initiation and development of these cancers. Here, we investigated the role of BMP-2 in colon cancer stem cell (CSC) development from colon cancer cells. We also determined the effects of BMP-2 on CSC development and epithelial-mesenchymal transition (EMT) in human colon cancer cell lines HCT-116 and SW620. We found that BMP-2 enhanced sphere formation of colon cancer cells without serum...
December 2015: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/26033745/osteopontin-promotes-hepatic-progenitor-cell-expansion-and-tumorigenicity-via-activation-of-%C3%AE-catenin-in-mice
#13
Yingying Liu, Lei Cao, Rui Chen, Xuyu Zhou, Xiaoyu Fan, Yingchao Liang, Rongjie Jia, Hao Wang, Guoke Liu, Yajun Guo, Jian Zhao
Upregulation of osteopontin (OPN) has been found in hepatic progenitor cells (HPCs) in several liver diseases with portal biliary proliferation. Here, we investigated the role of HPC-derived autocrine OPN in regulating HPC expansion, migration, and hepatocarcinogenesis in mice. Five-week-old, weighing between 18 and 20 g of either wild type (WT) or OPN gene knockout (OPN-KO) male mice were treated with modified choline-deficient, ethionine-supplemented diet (modified choline-deficient [MCDE]) for 2 weeks to induce HPC production, or 6-12 months to induce tumorigenesis...
December 2015: Stem Cells
https://www.readbyqxmd.com/read/25806288/individual-profiling-of-circulating-tumor-cell-composition-in-patients-with-non-small-cell-lung-cancer-receiving-platinum-based-treatment
#14
Ivonne Nel, Ulrich Jehn, Thomas Gauler, Andreas-Claudius Hoffmann
BACKGROUND: Circulating tumor cells (CTC) could serve as a "liquid biopsy" for individualizing and monitoring treatment in patients with solid tumors as recently shown by our group. We assessed which non-hematopoietic cell types are identifiable in the peripheral blood of patients with non-small cell lung cancer (NSCLC) and correlated those to clinical characteristics. METHODS: Blood from NSCLC patients (n=43) was processed as previously described. For subtype analyses CTC were negatively enriched by hematopoietic cell depletion...
April 2014: Translational Lung Cancer Research
https://www.readbyqxmd.com/read/25535895/a-breast-cancer-cell-microarray-cma-as-a-rapid-method-to-characterize-candidate-biomarkers
#15
Xinyan Wu, Muhammad S Zahari, Santosh Renuse, Harrys K C Jacob, Sruthi Sakamuri, Mukul Singal, Edward Gabrielson, Saraswati Sukumar, Akhilesh Pandey
Tissue microarrays (TMAs) have become an invaluable tool in cancer research to evaluate expression and subcellular localization of proteins in cells and tissues. As the catalogs of candidate biomarkers and therapeutic targets become more extensive, there is a need to characterize and validate these targets and biomarkers in cell lines as a primary biological system in research laboratories. Thus, cell microarrays (CMAs) are useful as a high-throughput screening tool. Here, we constructed a CMA containing 32 publicly available immortalized breast cell lines with the goal of creating a method to rapidly screen for antigens of interest in breast cancer research in a relatively easy, rapid and cost-effective manner...
2014: Cancer Biology & Therapy
https://www.readbyqxmd.com/read/25533099/cell-adhesion-and-urothelial-bladder-cancer-the-role-of-cadherin-switching-and-related-phenomena
#16
REVIEW
Richard T Bryan
Cadherins are mediators of cell-cell adhesion in epithelial tissues. E-cadherin is a known tumour suppressor and plays a central role in suppressing the invasive phenotype of cancer cells. However, the abnormal expression of N- and P-cadherin ('cadherin switching', CS) has been shown to promote a more invasive and m̀alignant phenotype of cancer, with P-cadherin possibly acting as a key mediator of invasion and metastasis in bladder cancer. Cadherins are also implicated in numerous signalling events related to embryonic development, tissue morphogenesis and homeostasis...
February 5, 2015: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/25502497/pancreatic-ductal-adenocarcinoma-cell-lines-display-a-plastic-ability-to-bi%C3%A2-directionally-convert-into-cancer-stem-cells
#17
Elisa Dalla Pozza, Ilaria Dando, Giulia Biondani, Jessica Brandi, Chiara Costanzo, Elisa Zoratti, Matteo Fassan, Federico Boschi, Davide Melisi, Daniela Cecconi, Maria Teresa Scupoli, Aldo Scarpa, Marta Palmieri
Pancreatic ductal adenocarcinoma (PDAC) is often diagnosed when metastatic events have occurred. Cancer stem cells (CSCs) play an important role in tumor initiation, metastasis, chemoresistance and relapse. A growing number of studies have suggested that CSCs exist in a dynamic equilibrium with more differentiated cancer cells via a bi‑directional regeneration that is dependent on the environmental stimuli. In this investigation, we obtain, by using a selective medium, PDAC CSCs from five out of nine PDAC cell lines, endowed with different tumorsphere‑forming ability...
March 2015: International Journal of Oncology
https://www.readbyqxmd.com/read/25487874/cell-surface-vimentin-a-mislocalized-protein-for-isolating-csvimentin-cd133-novel-stem-like-hepatocellular-carcinoma-cells-expressing-emt-markers
#18
Abhisek Mitra, Arun Satelli, Xueqing Xia, Jeffrey Cutrera, Lopa Mishra, Shulin Li
Recent advances in cancer stem cell biology have shown that cancer stem-like cells with epithelial-mesenchymal transition (EMT) phenotypes are more aggressive and cause relapse; however, absence of a specific marker to isolate these EMT stem-like cells hampers research in this direction. Cell surface markers have been identified for isolating cancer stem-like cells, but none has been identified for isolating cancer stem-like cells with EMT phenotype. Recently, we discovered that Vimentin, an intracellular EMT tumor cell marker, is present on the surface of colon metastatic tumor nodules in the liver...
July 15, 2015: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/25337967/-cancer-stem-cell-phenotypes-and-mirna-therapeutic-targets-in-head-and-neck-squamous-cell-carcinoma
#19
REVIEW
A Coordes, S Zhifeng, V Sangvatanakul, X Qian, M Lenarz, A M Kaufmann, A E Albers
BACKGROUND: Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer worldwide. HNSCC is caused by persistent high-risk human papillomavirus (HR-HPV) infection or excessive consumption of alcohol or tobacco. The persistently low survival rates result from local recurrences and metastases, which are probably caused by so-called tumor stem cells (TSCs). The epithelial-mesenchymal transition (EMT) or transformation is a key event in metastasis initiation and is being increasingly associated with TSCs...
December 2014: HNO
https://www.readbyqxmd.com/read/25261470/microrna-141-and-microrna-146b-5p-inhibit-the-prometastatic-mesenchymal-characteristics-through-the-rna-binding-protein-auf1-targeting-the-transcription-factor-zeb1-and-the-protein-kinase-akt
#20
Huda H Al-Khalaf, Abdelilah Aboussekhra
miR-141 and miR-146b-5p are two important tumor suppressor microRNAs, which control several cancer-related genes and processes. In the present report, we have shown that these microRNAs bind specific sites at the 3'-untranslated region (UTR) of the mRNA-binding protein AUF1, leading to its down-regulation. This inverse correlation between the levels of these microRNAs and AUF1 has been identified in various osteosarcoma cell lines. Additionally, we present clear evidence that AUF1 promotes mesenchymal features in osteosarcoma cells and that miR-141 and miR-146b-5p suppress this prometastatic process through AUF1 repression...
November 7, 2014: Journal of Biological Chemistry
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