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environmental microbiology

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https://www.readbyqxmd.com/read/28100597/investigation-of-a-candida-guilliermondii-pseudo-outbreak-reveals-a-novel-source-of-laboratory-contamination
#1
James E Kirby, Westyn Branch-Elliman, Mary T LaSalvia, Lorinda Longhi, Matthew MacKechnie, Grigoriy Urman, Linda M Baldini, Fatima R Muriel, Bernadette Sullivan, David S Yassa, Howard S Gold, Trevor K Wagner, Daniel J Diekema, Sharon B Wright
Candida guilliermondii was isolated from sterile specimens with increasing frequency over a several-month period despite a paucity of clinical evidence suggesting true Candida infections. However, a healthcare-associated outbreak was strongly considered due to growth patterns in the microbiology laboratory more consistent with true infection than environmental contamination. Therefore, an extensive investigation was performed to identify its cause. With the exception of one case, patient clinical courses were not consistent with true invasive fungal infections...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28067541/listeria-monocytogenes-circulating-in-rabbit-meat-products-and-slaughterhouses-in-italy-prevalence-data-and-comparison-among-typing-results
#2
Alessandra De Cesare, Antonio Parisi, Renzo Mioni, Damiano Comin, Alex Lucchi, Gerardo Manfreda
Rabbit meat has outstanding dietetic and nutritional properties. However, few data on microbiological hazards associated with rabbit productions are available. In this study, the presence of Listeria monocytogenes was determined in 430 rabbit carcasses, 256 rabbit meat cuts and products, and 599 environmental sponges collected from four Italian rabbit slaughterhouses over a period of 1 year. Prevalence of L. monocytogenes among the 1285 rabbit meat and environmental samples was 11%, with statistically significant differences between slaughterhouses...
January 9, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28063834/statistical-assessment-of-dumpsite-soil-suitability-to-enhance-methane-bio-oxidation-under-interactive-influence-of-substrates-and-temperature
#3
Somvir Bajar, Anita Singh, C P Kaushik, Anubha Kaushik
Biocovers are considered as the most effective and efficient way to treat methane (CH4) emission from dumpsites and landfills. Active methanotrophs in the biocovers play a crucial role in reduction of emissions through microbiological methane oxidation. Several factors affecting methane bio-oxidation (MOX) have been well documented, however, their interactive effect on the oxidation process needs to be explored. Therefore, the present study was undertaken to investigate the suitability of a dumpsite soil to be employed as biocover, under the influence of substrate concentrations (CH4 and O2) and temperature at variable incubation periods...
January 4, 2017: Waste Management
https://www.readbyqxmd.com/read/28063147/genetic-relatedness-of-faecal-coliforms-and-enterococci-bacteria-isolated-from-water-and-sediments-of-the-apies-river-gauteng-south-africa
#4
Mutshiene Deogratias Ekwanzala, Akebe Luther King Abia, Eunice Ubomba-Jaswa, Jitendra Keshri, Ndombo Benteke Maggy Momba
To date, the microbiological quality of river sediments and its impact on water resources are not included in the water quality monitoring assessment. Therefore, the aim of this study was to establish genetic relatedness between faecal coliforms and enterococci isolated from the river water and riverbed sediments of Apies River to better understand the genetic similarity of microorganisms between the sediment and water phases. Indicator bacteria were subjected to a molecular study, which consisted of PCR amplification and sequence analysis of the 16S rRNA and 23S rRNA gene using specific primers for faecal coliforms and enterococci, respectively...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28059049/evaluation-of-the-3m%C3%A2-molecular-detection-assay-mda-2-listeria-monocytogenes-for-the-detection-of-listeria-monocytogenes-in-select-foods-and-environmental-surfaces-collaborative-study-first-action-2016-08
#5
Patrick Bird, Jonathan Flannery, Erin Crowley, James Agin, David Goins, Lisa Monteroso
The 3M<sup>™</sup> Molecular Detection Assay (MDA) 2 <em>- Listeria </em>uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect <em>Listeria </em>spp. in a broad range of food types and environmental surfaces. Using an unpaired study design, the MDA 2 - <em>Listeria </em>was compared to the U.S. Department of Agriculture Food Safety and Inspection Service <em>Microbiology Laboratory Guidebook </em>Chapter 8.09 "<em>Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products, and Environmental Samples</em>" reference method for the detection of <em>Listeria </em>in deli turkey and raw chicken breast fillet...
November 17, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28058248/first-multi-year-retrospective-study-on-vibrio-parhaemolyticus-and-vibrio-vulnificus-prevalence-in-ruditapes-philippinarum-harvested-in-sacca-di-goro-italy
#6
Patrizia Serratore, Fabio Ostanello, Pier Luca Passalacqua, Emanuele Zavatta, Giorgia Bignami, Andrea Serraino, Federica Giacometti
The present work describes a retrospective study aiming to verify a possible correlation between the environmental conditions (temperature, salinity and dissolved oxygen), the abundance of Vibrio spp., and the prevalence of V. parahaemolyticus and V. vulnificus in the Manila clam R. philippinarum harvested in Sacca di Goro, Emilia-Romagna Region, Northern Italy. On the whole, 104 samples, collected in the period 2007-2015 and submitted to microbiological analyses (isolation and genotyping), have been reconsidered for Vibrio spp...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28058247/microbiological-contamination-in-three-large-scale-pig-slaughterhouses-in-northern-italy
#7
Pierluigi Di Ciccio, Maria Cristina Ossiprandi, Emanuela Zanardi, Sergio Ghidini, Giancarlo Belluzzi, Alberto Vergara, Adriana Ianieri
The aim of this survey was to obtain data on microbiological contamination of pig carcasses and environments in three large-scale Italian slaughterhouses (identified as A-B-C) located in Northern Italy. Each slaughterhouse was visited six times. Five carcasses and three different sites of the slaughterhouse (before and during slaughter) were sampled on each sampling day. A single pooled caecal sample was taken on each sampling day. A total of 90 carcasses, 108 environmental samples and 18 caecal samples were collected...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/28054956/a-strategy-to-establish-a-quality-assurance-quality-control-plan-for-the-application-of-biosensors-for-the-detection-of-e-coli-in-water
#8
Nikou Hesari, Nursel Kıratlı Yılmazçoban, Mohamad Elzein, Absar Alum, Morteza Abbaszadegan
Rapid bacterial detection using biosensors is a novel approach for microbiological testing applications. Validation of such methods is an obstacle in the adoption of new bio-sensing technologies for water testing. Therefore, establishing a quality assurance and quality control (QA/QC) plan is essential to demonstrate accuracy and reliability of the biosensor method for the detection of E. coli in drinking water samples. In this study, different reagents and assay conditions including temperatures, holding time, E...
January 3, 2017: Biosensors
https://www.readbyqxmd.com/read/28054272/enhanced-bacterial-affinity-of-pvdf-membrane-its-application-as-improved-sea-water-sampling-tool-for-environmental-monitoring
#9
Sweta Binod Kumar, Preeti Sharnagat, Paramita Manna, Amit Bhattacharya, Soumya Haldar
Isolation of diversified bacteria from seawater is a major challenge in the field of environmental microbiology. In the present study, an attempt has been made to select specific membrane with improved property of attaching diversified bacteria. Initially, different concentrations (15, 18, and 20% W/W) of polysulfone (PSF) were used to check their affinity for the attachment of selected gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. Among these, 20% W/W PSF showed maximum attachment...
January 5, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28050814/what-orthopaedic-operating-room-surfaces-are-contaminated-with-bioburden-a-study-using-the-atp-bioluminescence-assay
#10
Raveesh Daniel Richard, Thomas R Bowen
BACKGROUND: Contaminated operating room surfaces can increase the risk of orthopaedic infections, particularly after procedures in which hardware implantation and instrumentation are used. The question arises as to how surgeons can measure surface cleanliness to detect increased levels of bioburden. This study aims to highlight the utility of adenosine triphosphate (ATP) bioluminescence technology as a novel technique in detecting the degree of contamination within the sterile operating room environment...
January 3, 2017: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/28040844/application-of-organic-acids-for-plant-protection-against-phytopathogens
#11
REVIEW
Igor G Morgunov, Svetlana V Kamzolova, Emilia G Dedyukhina, Tatiana I Chistyakova, Julia N Lunina, Alexey A Mironov, Nadezda N Stepanova, Olga N Shemshura, Mikhail B Vainshtein
The basic tendency in the field of plant protection concerns with reducing the use of pesticides and their replacement by environmentally acceptable biological preparations. The most promising approach to plant protection is application of microbial metabolites. In the last years, bactericidal, fungicidal, and nematodocidal activities were revealed for citric, succinic, α-ketoglutaric, palmitoleic, and other organic acids. It was shown that application of carboxylic acids resulted in acceleration of plant development and the yield increase...
February 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28040195/human-health-screening-and-public-health-significance-of-contaminants-of-emerging-concern-detected-in-public-water-supplies
#12
Robert Benson, Octavia D Conerly, William Sander, Angela L Batt, J Scott Boone, Edward T Furlong, Susan T Glassmeyer, Dana W Kolpin, Heath E Mash, Kathleen M Schenck, Jane Ellen Simmons
The source water and treated drinking water from twenty five drinking water treatment plants (DWTPs) across the United States were sampled in 2010-2012. Samples were analyzed for 247 contaminants using 15 chemical and microbiological methods. Most of these contaminants are not regulated currently either in drinking water or in discharges to ambient water by the U. S. Environmental Protection Agency (USEPA) or other U.S. regulatory agencies. This analysis shows that there is little public health concern for most of the contaminants detected in treated water from the 25 DWTPs participating in this study...
February 1, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28004529/web-alert-drugs-from-marine-microbes-an-annotated-selection-of-world-wide-web-sites-relevant-to-the-topics-in-environmental-microbiology
#13
Lawrence P Wackett
No abstract text is available yet for this article.
December 2016: Environmental Microbiology
https://www.readbyqxmd.com/read/28001363/mass-spectrometry-imaging-of-complex-microbial-communities
#14
Sage J B Dunham, Joseph F Ellis, Bin Li, Jonathan V Sweedler
In the two decades since mass spectrometry imaging (MSI) was first applied to visualize the distribution of peptides across biological tissues and cells, the technique has become increasingly effective and reliable. MSI excels at providing complementary information to existing methods for molecular analysis-such as genomics, transcriptomics, and metabolomics-and stands apart from other chemical imaging modalities through its capability to generate information that is simultaneously multiplexed and chemically specific...
December 21, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27995098/evaluation-of-hygienic-quality-and-labelling-of-fish-distributed-in-public-canteens-of-northeast-italy
#15
Mariachiara Armani, Michele Civettini, Gabriella Conedera, Michela Favretti, Dorotea Lombardo, Rosaria Lucchini, Sabrina Paternolli, Alessandra Pezzuto, Michela Rabini, Giuseppe Arcangeli
Over the past few years, the demand for the introduction of fish products in public canteens (schools, hospitals and nursing-homes) has grown due to their good nutritional proprieties. The particular health conditions and sensitivity of some groups of consumers exposes them to greater risks of food poisoning. It is therefore important to monitor the raw materials that end up in mass catering implementing strategies of mass catering control, both with self-monitoring strategies and with regular controls performed by the competent health authorities...
September 20, 2016: Italian Journal of Food Safety
https://www.readbyqxmd.com/read/27989610/microbiological-quality-of-honey-from-the-pampas-region-argentina-throughout-the-extraction-process
#16
Leticia A Fernández, Carolina Ghilardi, Betiana Hoffmann, Carlos Busso, Liliana M Gallez
The microbiological quality of honey obtained from different processing points and the environmental quality within honey houses were assessed in the Pampas Region (Argentina). Mold and yeast (MY), culturable heterotrophic mesophilic bacteria (CHMB), the number of spore-forming bacteria as well as the presence of Shigella spp., Salmonella spp. and fecal coliforms were evaluated in 163 samples. These samples were taken from eight honey houses. Results showed that 89 samples had ≤10CFU of MY/g honey, 69 ranged from 10 to 50CFU/g and two reached 65...
December 16, 2016: Revista Argentina de Microbiología
https://www.readbyqxmd.com/read/27974738/significance-of-viable-but-nonculturable-escherichia-coli-induction-detection-and-control
#17
REVIEW
Tian Ding, Yuanjie Suo, Qisen Xiang, Xihong Zhao, Shiguo Chen, Xingqian Ye, Donghong Liu
Diseases caused by foodborne or waterborne pathogens are emerging. Many pathogens can enter into the viable but nonculturable (VBNC) state, which is a survival strategy when exposed to harsh environmental stresses. Pathogens in the VBNC state have the ability to evade conventional microbiological detection methods that pose a significant and potential health risk. Therefore, controlling the VBNC bacteria in food processing and environment is of great importance. As the typical one of the Gram-negatives, Escherichia coli (E...
December 14, 2016: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/27965958/a-dormant-microbial-component-in-the-development-of-preeclampsia
#18
REVIEW
Douglas B Kell, Louise C Kenny
Preeclampsia (PE) is a complex, multisystem disorder that remains a leading cause of morbidity and mortality in pregnancy. Four main classes of dysregulation accompany PE and are widely considered to contribute to its severity. These are abnormal trophoblast invasion of the placenta, anti-angiogenic responses, oxidative stress, and inflammation. What is lacking, however, is an explanation of how these themselves are caused. We here develop the unifying idea, and the considerable evidence for it, that the originating cause of PE (and of the four classes of dysregulation) is, in fact, microbial infection, that most such microbes are dormant and hence resist detection by conventional (replication-dependent) microbiology, and that by occasional resuscitation and growth it is they that are responsible for all the observable sequelae, including the continuing, chronic inflammation...
2016: Frontiers in Medicine
https://www.readbyqxmd.com/read/27964775/hygiene-on-maternity-units-lessons-from-a-needs-assessment-in-bangladesh-and-india
#19
Suzanne Cross, Kaosar Afsana, Morsheda Banu, Dileep Mavalankar, Emma Morrison, Atiya Rahman, Tapash Roy, Deepak Saxena, Kranti Vora, Wendy J Graham
BACKGROUND: As the proportion of deliveries in health institutions increases in low- and middle-income countries, so do the challenges of maintaining standards of hygiene and preventing healthcare-associated infections (HCAIs) in mothers and babies. Adequate water, sanitation, and hygiene (WASH) and infection prevention and control (IPC) in these settings should be seen as integral parts of the broader domain of quality care. Assessment approaches are needed which capture standards for both WASH and IPC, and so inform quality improvement processes...
2016: Global Health Action
https://www.readbyqxmd.com/read/27959960/nontuberculous-mycobacterial-infections-in-a-french-hospital-a-12-year-retrospective-study
#20
Peggy Blanc, Hervé Dutronc, Olivia Peuchant, Frédéric-Antoine Dauchy, Charles Cazanave, Didier Neau, Gaëtane Wirth, Jean-Luc Pellegrin, Philippe Morlat, Patrick Mercié, José-Manuel Tunon-de-Lara, Marie-Sylvie Doutre, Philippe Pélissier, Michel Dupon
BACKGROUND: Nontuberculous mycobacteria (NTM) are environmental organisms associated with a range of infections. Reports of NTM epidemiology are mainly focused on pulmonary infections and isolations, and extrapulmonary infections are less frequently described. METHODS: We conducted a retrospective study of NTM infections at the Bordeaux University Hospital, France, between January 2002 and December 2013. We used the microbiologic component of the American Thoracic Society/Infectious Diseases Society of America's pulmonary NTM disease criteria to define cases of pulmonary NTM, and patients with isolates from a normally sterile site were classified as having extrapulmonary disease...
2016: PloS One
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