keyword
MENU ▼
Read by QxMD icon Read
search

Ki67 breast cancer

keyword
https://www.readbyqxmd.com/read/29329585/involvement-of-hedgehog-pathway-in-early-onset-aggressive-molecular-subtypes-and-metastatic-potential-of-breast-cancer
#1
Syeda Kiran Riaz, Jahangir Sarwar Khan, Syed Tahir Abbas Shah, Fen Wang, Lin Ye, Wen G Jiang, Muhammad Faraz Arshad Malik
BACKGROUND: Dysregulation of hedgehog pathway is observed in numerous cancers. Relevance of hedgehog pathway genes in cancer cohort and inhibition of its downstream effector (GLI1) towards metastasis in cell lines are explored in the study. METHOD: One hundred fifty fresh tumours of breast cancer patients were collected for the study. Based on differential expression, panel of 6 key regulators of the pathway (SHH, DHH, IHH, PTCH1, SMO and GLI1) in microarray datasets were identified...
January 8, 2018: Cell Communication and Signaling: CCS
https://www.readbyqxmd.com/read/29326029/neoadjuvant-treatment-with-trastuzumab-and-pertuzumab-plus-palbociclib-and-fulvestrant-in-her2-positive-er-positive-breast-cancer-na-pher2-an-exploratory-open-label-phase-2-study
#2
Luca Gianni, Giancarlo Bisagni, Marco Colleoni, Lucia Del Mastro, Claudio Zamagni, Mauro Mansutti, Milvia Zambetti, Antonio Frassoldati, Raffaella De Fato, Pinuccia Valagussa, Giuseppe Viale
BACKGROUND: In the neoadjuvant setting, blockade of HER2 plus use of an aromatase inhibitor in patients with HER2-positive and oestrogen receptor (ER)-positive breast cancer leads to a pathological complete response in 21% of patients. Convergence of HER2 and ER signals on RB1 suggests that a combined pharmacological intervention directed to these targets could be synergistic. To test this approach, we combined palbociclib to block RB1, fulvestrant to block ER, and trastuzumab with pertuzumab to block HER2 in patients with HER2-positive, ER-positive breast cancer...
January 8, 2018: Lancet Oncology
https://www.readbyqxmd.com/read/29311658/local-delivery-of-hormonal-therapy-with-silastic-tubing-for-prevention-and-treatment-of-breast-cancer
#3
Jeenah Park, Scott Thomas, Allison Y Zhong, Alan R Wolfe, Gregor Krings, Manuela Terranova-Barberio, Nela Pawlowska, Leslie Z Benet, Pamela N Munster
Broad use of germline testing has identified an increasing number of women at risk for breast cancer with a need for effective chemoprevention. We report a novel method to selectively deliver various anti-estrogens at high drug levels to the breast tissue by implanting a device comprised of silastic tubing. Optimized tubing properties allow elution of otherwise poorly bioavailable anti-estrogens, such as fulvestrant, into mammary tissue in vitro and in vivo with levels sufficient to inhibit estrogen receptor activation and tumor cell proliferation...
January 8, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29304138/the-use-of-automated-ki67-analysis-to-predict-oncotype-dx-risk-of-recurrence-categories-in-early-stage-breast-cancer
#4
Satbir Singh Thakur, Haocheng Li, Angela M Y Chan, Roxana Tudor, Gilbert Bigras, Don Morris, Emeka K Enwere, Hua Yang
Ki67 is a commonly used marker of cancer cell proliferation, and has significant prognostic value in breast cancer. In spite of its clinical importance, assessment of Ki67 remains a challenge, as current manual scoring methods have high inter- and intra-user variability. A major reason for this variability is selection bias, in that different observers will score different regions of the same tumor. Here, we developed an automated Ki67 scoring method that eliminates selection bias, by using whole-slide analysis to identify and score the tumor regions with the highest proliferative rates...
2018: PloS One
https://www.readbyqxmd.com/read/29301832/pharmacological-inhibition-of-nos-activates-ask1-jnk-pathway-augmenting-docetaxel-mediated-apoptosis-in-triple-negative-breast-cancer
#5
Daniel Dávila-González, Dong Soon Choi, Roberto R Rosato, Sergio Granados-Principal, John G Kuhn, Wen-Feng Li, Wei Qian, Wen Chen, Anthony J Kozielski, Helen H Wong, Bhuvanesh Dave, Jenny C Chang
PURPOSE: Chemoresistance in triple negative breast cancer (TNBC) is associated with the activation of a survival mechanism orchestrated by the endoplasmic reticulum (EnR) stress response and by inducible nitric oxide synthase (iNOS). Our aim was to determine the effects of pharmacological NOS-inhibition on TNBC. EXPERIMENTAL DESIGN: TNBC cell lines, SUM-159PT, MDA-MB-436, and MDA-MB-468, were treated with docetaxel and NOS-inibitor (L-NMMA) for 24, 48 and 72 hours...
January 4, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29298285/the-relationship-between-breast-cancer-treatment-tumour-type-and-vitamin-d-level-in-pre-and-postmenopausal-women
#6
Joanna Streb, Izabela Glanowska, Anna Streb, Joanna Szpor, Koleta Kryka, Paweł Potocki, Małgorzata Wójcik, Robert Jach
OBJECTIVE: Vitamin D deficiency has been linked to a higher risk of cancer. There is insufficient data regarding the influence of treatment on vitamin D status. The aim of this study was to compare pre- and post-treatment levels of 25(OH)D in premenopausal and postmenopausal women with breast cancer with a different receptor status (ER-estrogen receptors, PR-progesterone receptors) and in healthy controls. METHODS: 49 patients with breast cancer (23 premenopausal), and 28 healthy controls matched for age, menopausal status and BMI...
December 4, 2017: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/29286917/protein-and-dna-evidences-of-hcmv-infection-in-primary-breast-cancer-tissues-and-metastatic-sentinel-lymph-nodes
#7
Jian Cui, Qian Wang, Hai-Bo Wang, Bin Wang, Ling Li
BACKGROUND AND OBJECTIVE: Breast cancer is the leading cause of death in women worldwide. There are evidences that human cytomegalovirus (HCMV) infection is associated with several malignant tumors. This study aims to investigate the infection of human cytomegalovirus (HCMV) in a large sample of breast cancer patients, and conduct a correlation analysis of clinical and pathological factors, to provide evidence for whether HCMV infection is associated with breast cancer development, progression and metastasis...
December 19, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29286417/building-up-a-high-throughput-screening-platform-to-assess-the-heterogeneity-of-her2-gene-amplification-in-breast-cancers
#8
Giulia Ercoli, Gianluca Lopez, Camilla Ciapponi, Chiara Corti, Luca Despini, Donatella Gambini, Letterio Runza, Concetta Blundo, Amedeo Sciarra, Nicola Fusco
Targeted therapies against the human epidermal growth factor receptor 2 (HER2) have radically changed the outcome of patients with HER2-positive breast cancers. However, a minority of cases displays a heterogeneous distribution of HER2-positive cells, which generates major clinical challenges. To date, no reliable and standardized protocols for the characterization and quantification of HER2 heterogeneous gene amplification in large cohorts have been proposed. Here, we present a high-throughput methodology to simultaneously assess the HER2 status across different topographic areas of multiple breast cancers...
December 5, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284000/oestrogen-receptor-negative-early-operable-primary-breast-cancer-in-older-women-biological-characteristics-and-long-term-clinical-outcome
#9
Binafsha Manzoor Syed, Dal Morgan, Tulassi Setty, Andrew R Green, Emma C Paish, Ian O Ellis, K L Cheung
BACKGROUND: Older women are at the greatest risk of breast cancer development and a considerable number present with comorbidities. Although the majority of breast cancers in this age group express oestrogen receptor (ER), which makes endocrine therapy (primary or adjuvant) feasible, given the huge size of the elderly population, there remains a significant number of patients, in absolute term, whose tumours do not express ER and their management is challenging. METHODS: Of a consecutive series of 1,758 older (≥70 years) women with early operable primary breast cancer managed in a dedicated service from 1973-2010, 252(14...
2017: PloS One
https://www.readbyqxmd.com/read/29283514/an-animal-model-of-local-breast-cancer-recurrence-in-the-setting-of-autologous-fat-grafting-for-breast-reconstruction
#10
Wakako Tsuji, Jolene E Valentin, Kacey G Marra, Albert D Donnenberg, Vera S Donnenberg, J Peter Rubin
Autologous fat grafting after breast cancer surgery is commonly performed, but concerns about oncologic risk remain. To model the interaction between fat grafting and breast cancer cells, two approaches were employed. In the first approach, graded numbers of viable MDA-MB-231 or BT-474 cells were admixed directly into human fat grafts and injected subcutaneously into immune-deficient mice to determine if the healing graft is a supportive environment for the tumor. In the second approach, graded doses of MDA-MB-231 cells were suspended in Matrigel and injected into the mammary fat pads of mice...
January 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29275302/evaluation-of-vascular-proliferation-in-molecular-subtypes-of-breast-cancer
#11
Ionela Sevilla Bujor, Andreea Cioca, Raluca Amalia Ceaușu, Fulga Veaceslav, Cristian Nica, Anca Maria Cîmpean, Marius Raica
BACKGROUND: Angiogenesis plays a pivotal role in tumor development. Although microvessel density (MVD) is the most common method used for evaluation of angiogenesis, it has several limitations. Our aim was to evaluate MVD and microvessel proliferation (MVP) in a series of invasive breast carcinomas and analyze whether angiogenesis is influenced by the molecular phenotype of each tumor. MATERIALS AND METHODS: We examined vascular proliferation using double immunohistochemistry (CD34/Ki67) in a series of 54 invasive breast carcinomas and compared the results with standard MVD, molecular subtypes and other classical parameters...
January 2018: In Vivo
https://www.readbyqxmd.com/read/29251737/integrated-tumor-identification-and-automated-scoring-minimizes-pathologist-involvement-and-provides-new-insights-to-key-biomarkers-in-breast-cancer
#12
Peter Bankhead, José A Fernández, Darragh G McArt, David P Boyle, Gerald Li, Maurice B Loughrey, Gareth W Irwin, D Paul Harkin, Jacqueline A James, Stephen McQuaid, Manuel Salto-Tellez, Peter W Hamilton
Digital image analysis (DIA) is becoming central to the quantitative evaluation of tissue biomarkers for discovery, diagnosis and therapeutic selection for the delivery of precision medicine. In this study, automated DIA using a new purpose-built software platform (QuPath) is applied to a cohort of 293 breast cancer patients to score five biomarkers in tissue microarrays (TMAs): ER, PR, HER2, Ki67 and p53. This software is able to measure IHC expression following fully automated tumor recognition in the same immunohistochemical (IHC)-stained tissue section, as part of a rapid workflow to ensure objectivity and accelerate biomarker analysis...
December 18, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/29248134/pilot-study-demonstrating-metabolic-and-anti-proliferative-effects-of-in-vivo-anti-oxidant-supplementation-with-n-acetylcysteine-in-breast-cancer
#13
Daniel Monti, Federica Sotgia, Diana Whitaker-Menezes, Madalina Tuluc, Ruth Birbe, Adam Berger, Melissa Lazar, Paolo Cotzia, Rossitza Draganova-Tacheva, Zhao Lin, Marina Domingo-Vidal, Andrew Newberg, Michael P Lisanti, Ubaldo Martinez-Outschoorn
BACKGROUND: High oxidative stress as defined by hydroxyl and peroxyl activity is often found in the stroma of human breast cancers. Oxidative stress induces stromal catabolism, which promotes cancer aggressiveness. Stromal cells exposed to oxidative stress release catabolites such as lactate, which are up-taken by cancer cells to support mitochondrial oxidative phosphorylation. The transfer of catabolites between stromal and cancer cells leads to metabolic heterogeneity between these cells and increased cancer cell proliferation and reduced apoptosis in preclinical models...
June 2017: Seminars in Oncology
https://www.readbyqxmd.com/read/29230610/predictive-value-of-vascular-endothelial-growth-factor-receptor-type-2-in-triple-negative-breast-cancer-patients-treated-with-neoadjuvant-chemotherapy
#14
Nataliya Babyshkina, Marina Zavyalova, Natalia Tarabanovskaya, Tatyana Dronova, Nadejda Krakhmal, Elena Slonimskaya, Julia Kzhyshkowska, Evgeny Choynzonov, Nadejda Cherdyntseva
The identification of informative biomarkers that could predict the treatment response is particularly important in the triple-negative (TN) breast cancer, which is characterized by biological diversity. The aim of this study was to investigate the impact of vascular endothelial growth factor receptor (VEGFR2) expression and its gene polymorphisms on pathologic complete response (pCR) to neoadjuvant chemotherapy (NCT) in Russian patients with TN breast cancer. We performed a retrospective analysis of 70 women with operable TN breast cancer, who underwent NCT with 5-fluorouracil, adriamycin, and cyclophosphamide (FAC) or cyclophosphamide, adriamycin, and capecitabine (CAX) between 2007 and 2013...
December 11, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/29228411/management-of-breast-cancer-in-adjuvant-chemotherapy-settings-in-the-kanagawa-breast-oncology-group
#15
Mayako Terao, Naoki Niikura, Yasuhiro Suzuki, Norihiko Sengoku, Hitoshi Arioka, Takashi Ishikawa, Koichiro Tsugawa, Yutaka Tokuda
OBJECTIVE: Many different options for adjuvant chemotherapy are recommended in guidelines for the treatment of breast cancer. Therapeutic strategies vary among physicians. The major goals for this project were to gain a better understanding of how biomarkers are integrated into practice and how physicians select adjuvant chemotherapy. METHODS: We assembled a questionnaire with 23 example scenarios of breast cancer cases, including 6 items relevant to postoperative adjuvant therapy...
December 20, 2017: Tokai Journal of Experimental and Clinical Medicine
https://www.readbyqxmd.com/read/29220095/digital-image-analysis-of-ki67-in-hot-spots-is-superior-to-both-manual-ki67-and-mitotic-counts-in-breast-cancer
#16
Gustav Stålhammar, Stephanie Robertson, Lena Wedlund, Michael Lippert, Mattias Rantalainen, Jonas Bergh, Johan Hartman
AIMS: During pathological examination of breast tumours, proliferative activity is routinely evaluated by a count of mitoses. Adding immunohistochemical stains of Ki67 provides extra prognostic and predictive information. However, currently used methods for these evaluations suffer from imperfect reproducibility. It is still unclear whether analysis of Ki67 should be performed in hot spots, in the tumour periphery or as an average of the whole tumour section. In this paper, we compare the clinical relevance of mitoses, Ki67 and Phosphohistone H3 in two cohorts of primary breast cancer specimens (total n=294)...
December 8, 2017: Histopathology
https://www.readbyqxmd.com/read/29217733/sex-as-a-biological-variable-in-preclinical-imaging-research-initial-observations-with-18f-fluorothymidine
#17
Szeman Ruby Chan, Kelley Salem, Justin Jeffery, Ginny L Powers, Yongjun Yan, Kooresh I Shoghi, Aparna M Mahajan, Amy M Fowler
The study objective was to investigate whether sex influences 3'-deoxy-3'-[18F]fluorothymidine (18F-FLT) uptake and tissue distribution in mouse models of cancer. Methods:18F-FLT biodistribution was measured in three strains of male and female mice (129S6/SvEv, athymic nude, and BALB/c). 18F-Fluoro-2-deoxy-2-D-glucose (18F-FDG) biodistribution was performed for comparison. 18F-FLT uptake was also measured in female 129S6/SvEv mice bearing estrogen-dependent SSM3 mouse mammary tumors, male athymic nude mice bearing androgen-dependent CWR22 prostate cancer xenografts, and male and female athymic nude mice bearing estrogen-independent MDA-MB-231 human breast cancer xenografts...
December 7, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
https://www.readbyqxmd.com/read/29214214/molecular-changes-in-premenopausal-oestrogen-receptor-positive-primary-breast-cancer-in-vietnamese-women-after-oophorectomy
#18
Ben P Haynes, Ophira Ginsburg, Qiong Gao, Elizabeth Folkerd, Maria Afentakis, Le Hong Quang, Pham Thi Han, Pham Hong Khoa, Nguyen Van Dinh, Ta Van To, Mark Clemons, Ian E Smith, Mitch Dowsett
For premenopausal women with primary ER + breast cancer, oophorectomy (OvX) is an evidence-based cost-effective option and is standard treatment in many countries. However, there is virtually no data describing the effects of OvX on breast tumour biology. We therefore, characterised the endocrine and genome-wide transcriptional impact of OvX in 56 premenopausal women with ER + breast cancer for 2 weeks prior to mastectomy. Plasma estradiol concentrations decreased from 406 ± 41 to 20.7 ± 2...
2017: NPJ Breast Cancer
https://www.readbyqxmd.com/read/29212204/myc-overexpression-with-its-prognostic-and-clinicopathological-significance-in-breast-cancer
#19
Jingkun Qu, Xixi Zhao, Jizhao Wang, Xu Liu, Yan Yan, Lin Liu, Hui Cai, Hangying Qu, Ning Lu, Yuchen Sun, Feidi Wang, Jiansheng Wang, Jia Zhang
Background: Proto-oncogene MYC has been indicated to promote progression of many cancers. However, prognostic and clinicopathological significance of MYC in breast cancer need further evaluation. Methods: We searched EMBASE and PubMed databases to find useful studies. We analyzed relationships between high MYC expression and prognostic data/ clinicopathological features through hazard ratio (HR) and odds ratio (OR). Each statistical test was two-sided. Results: There were 29 studies (36 cohorts) with 12621 patients enrolled in our study The MYC overexpression was associated with worse DFS/RFS (disease/relapse free survival) in 11 studies (16 cohorts) with 5390 patients, and OS (overall survival) of 7 studies (8 cohorts) with 2672 patients...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29202622/an-inter-observer-ki67-reproducibility-study-applying-two-different-assessment-methods-on-behalf-of-the-danish-scientific-committee-of-pathology-danish-breast-cancer-cooperative-group-dbcg
#20
Anne-Vibeke Laenkholm, Dorthe Grabau, Maj-Lis Møller Talman, Eva Balslev, Anne Marie Bak Jylling, Tomasz Piotr Tabor, Morten Johansen, Anja Brügmann, Giedrius Lelkaitis, Tina Di Caterino, Henrik Mygind, Thomas Poulsen, Henrik Mertz, Gorm Søndergaard, Birgitte Bruun Rasmussen
INTRODUCTION: In 2011, the St. Gallen Consensus Conference introduced the use of pathology to define the intrinsic breast cancer subtypes by application of immunohistochemical (IHC) surrogate markers ER, PR, HER2 and Ki67 with a specified Ki67 cutoff (>14%) for luminal B-like definition. Reports concerning impaired reproducibility of Ki67 estimation and threshold inconsistency led to the initiation of this quality assurance study (2013-2015). The aim of the study was to investigate inter-observer variation for Ki67 estimation in malignant breast tumors by two different quantification methods (assessment method and count method) including measure of agreement between methods...
December 5, 2017: Acta Oncologica
keyword
keyword
72753
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"