keyword
MENU ▼
Read by QxMD icon Read
search

Odontoblast

keyword
https://www.readbyqxmd.com/read/28091877/influence-of-enamel-dentin-thickness-on-the-toxic-and-esthetic-effects-of-experimental-in-office-bleaching-protocols
#1
C C de Oliveira Duque, D G Soares, F G Basso, J Hebling, C A de Souza Costa
OBJECTIVES: This paper aims to assess the whitening effectiveness and toxicity of tooth-bleaching protocols applied to enamel/dentin disks simulating mandibular incisors (ICs) and premolars (PMs). MATERIALS AND METHODS: A 10% hydrogen peroxide (H2O2) gel was applied for 3 × 15, 1 × 15, or 1 × 5 min to enamel/dentin disks simulating mandibular ICs and PMs, and the trans-enamel and trans-dentinal diffusion products were applied to human dental pulp cells (1 h)...
January 14, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28081373/loss-of-function-of-evc2-in-dental-mesenchyme-leads-to-hypomorphic-enamel
#2
H Zhang, H Takeda, T Tsuji, N Kamiya, T Kunieda, Y Mochida, Y Mishina
Ellis-van Creveld (EvC) syndrome is an autosomal-recessive skeletal dysplasia, characterized by short stature and postaxial polydactyly. A series of dental abnormalities, including hypomorphic enamel formation, has been reported in patients with EvC. Despite previous studies that attempted to uncover the mechanism leading to abnormal tooth development, little is known regarding how hypomorphic enamel is formed in patients with EvC. In the current study, using Evc2/ Limbin mutant mice we recently generated, we analyzed enamel formation in the mouse incisor...
December 1, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/28081294/xenogeneic-bio-root-prompts-the-constructive-process-characterized-by-macrophage-phenotype-polarization-in-rodents-and-nonhuman-primates
#3
Hui Li, Jingjing Sun, Jie Li, Hefeng Yang, Xiangyou Luo, Jinlong Chen, Li Xie, Fangjun Huo, Tian Zhu, Weihua Guo, Weidong Tian
Tissue or organ regeneration using xenogeneic matrices is a promising approach to address the shortage of donor matrices for allotransplantation. Success of such approach has been demonstrated to correlate with macrophage-mediated fibrotic homeostasis and tissue remodeling. The previous studies have demonstrated that treated dentin matrix (TDM) could be a suitable bioactive substrate for allogeneic tooth root regeneration. This study constructed xenogeneic bioengineered tooth root (bio-root) via a combination of porcine TDM (pTDM) with allogeneic dental follicle cells (DFCs)...
January 12, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28043013/transient-receptor-potential-melastatin-trpm-8-is-expressed-in-freshly-isolated-native-human-odontoblasts
#4
Kento Tazawa, Hideharu Ikeda, Nobuyuki Kawashima, Takashi Okiji
OBJECTIVE: Cold-sensitive ion channels, such as transient receptor potential melastatin (TRPM) 8 and transient receptor potential ankyrin (TRPA) 1, may play a crucial role in the nociceptive function of odontoblasts, whereas expression of these TRP channels in human native odontoblasts remains to be elucidated. This study aimed to analyze the expression of TRPM8 and TRPA1 in freshly isolated native human odontoblasts. DESIGN: Odontoblasts were isolated from freshly extracted healthy human teeth (n=4); after removing the inner pulp tissues from the pulp chambers, odontoblasts remaining on the dentin surface were washed out with phosphate buffered saline and collected...
December 23, 2016: Archives of Oral Biology
https://www.readbyqxmd.com/read/28041686/evaluation-of-a-commercially-available-hyaluronic-acid-hydrogel-restylane-as-injectable-scaffold-for-dental-pulp-regeneration-an-in%C3%A2-vitro-evaluation
#5
Vanessa Chrepa, Obadah Austah, Anibal Diogenes
INTRODUCTION: Regenerative endodontic procedures (REPs) are viable alternatives for treating immature teeth, yet these procedures do not predictably lead to pulp-dentin regeneration. A true bioengineering approach for dental pulp regeneration requires the incorporation of a scaffold conducive with the regeneration of the pulp-dentin complex. Several materials have been proposed as scaffolds for REPs; nonetheless, the majority are not eligible for immediate clinical chairside use. Thus, the aim of this study was to evaluate Restylane, a Food and Drug Administration-approved hyaluronic acid-based gel, as possible scaffold for REPs...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28041683/the-role-of-transient-receptor-potential-cation-channel-subfamily-c-member-1-in-the-odontoblastlike-differentiation-of-human-dental-pulp-cells
#6
Zhi Song, Lingling Chen, Jia Guo, Wei Qin, Runfu Wang, Shuheng Huang, Xiaoting Yang, Yaguang Tian, Zhengmei Lin
INTRODUCTION: Calcium ions (Ca(2+)) actively participate in reparative dentin formation by promoting cellular proliferation and differentiation of human dental pulp cells (hDPCs). Transient receptor potential cation channel, subfamily C, member 1 (TRPC1) activates Ca(2+) entry upon store depletion in a variety of cell types. However, the function of TRPC1 in hDPCs has not been reported. Therefore, we aimed to analyze the role of TRPC1 in hDPCs undergoing odontoblastlike differentiation...
December 29, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/28000152/inactivation-of-bone-morphogenetic-protein-1-bmp1-and-tolloid-like-1-tll1-in-cells-expressing-type-i-collagen-leads-to-dental-and-periodontal-defects-in-mice
#7
Hua Zhang, Priyam Jani, Tian Liang, Yongbo Lu, Chunlin Qin
Bone morphogenetic protein 1 (BMP1) and tolloid-like 1 (TLL1) belong to the BMP1/tolloid-like proteinase family, which cleaves secretory proteins. The constitutive deletion of the Bmp1 or Tll1 genes causes perinatal or embryonic lethality in mice. In this study, we first studied the β-galactosidase activity in mice in which an IRES-lacZ-Neo cassette was inserted in the intron of either the Bmp1 or the Tll1 gene; the β-galactosidase activities were used to reflect the expression of endogenous Bmp1 and Tll1, respectively...
December 20, 2016: Journal of Molecular Histology
https://www.readbyqxmd.com/read/27989582/expression-of-cannabinoid-type-1-receptors-in%C3%A2-human-odontoblast-cells
#8
Kehua Que, Dan He, Ying Jin, Ligeng Wu, Fang Wang, Zhiying Zhao, Jing Yang, Jiayin Deng
INTRODUCTION: The aim of this study was to investigate the functional expression of cannabinoid type 1 (CB1) receptors in human odontoblasts (HODs) and the possible internal mechanism. METHODS: In the present study, we examined the molecular and functional expression of the CB1 receptors in cultured HOD-like cells and native HODs obtained from healthy wisdom teeth. RESULTS: Immunohistochemistry and immunofluorescence revealed that CB1 receptors localize to native HODs and HOD-like cells, respectively...
December 15, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27986375/biological-evaluation-of-a-new-pulp-capping-material-developed-from-portland-cement
#9
Ahmed M Negm, Ehab E Hassanien, Ashraf M Abu-Seida, Mohamed M Nagy
This study evaluates the biological properties of a new pulp capping material developed from Portland cement. This study was conducted on 48 teeth in 4 dogs (12 teeth/dog). The dogs were classified into two equal groups (n=24 teeth) according to the evaluation period including: group A (3 weeks) and group B (3 months). Each group was further subdivided into three equal subgroups (n=8 teeth) according to the capping material including: subgroup 1: mineral trioxide aggregate (MTA), subgroup2: Portland cement+10% calcium hydroxide+20% bismuth oxide (Port Cal) and subgroup 3: Portland cement+bismuth oxide...
December 13, 2016: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/27977859/immune-cell-profile-of-dental-pulp-tissue-treated-with-zoledronic-acid
#10
P G de Barros Silva, M E Q L Verde, L A C Brizeno, D V T Wong, R C P Lima Júnior, F B Sousa, M R L Mota, A P N N Alves
AIM: To characterize the pulp immune cell profile in the teeth of rats treated with zoledronic acid (ZA). METHODOLOGY: Male Wistar rats (n = 6 per group) received four intravenous infusions of ZA at doses of 0.04, 0.20 or 1.00 mg kg(-1) ZA or saline (control). On the 70th experimental day, they were euthanized. The first right molar was examined microscopically and submitted to toluidine blue reaction and immunohistochemical for CD68, tumour necrosis Factor (TNF)-α, interleukin (IL)-1β, inducible nitric oxide synthase (iNOS), nuclear factor kappa B (NF-kB) and IL-18 binding protein (IL-18 bp)...
December 15, 2016: International Endodontic Journal
https://www.readbyqxmd.com/read/27928103/biological-efficacy-of-two-mineral-trioxide-aggregate-mta-based-materials-in-a-canine-model-of-pulpotomy
#11
Myeongyeon Lee, Chung-Min Kang, Je Seon Song, Yooseok Shin, Seunghye Kim, Seong-Oh Kim, Hyung-Jun Choi
The aim of this study was to compare the biocompatibility of Endocem Zr(®) and ProRoot MTA(®) by histopathologic analysis in a canine model of pulpotomy. This study utilized 39 teeth of two beagle dogs. The exposed pulp tissues were treated by pulpotomy using ProRoot MTA (n=19) or Endocem Zr (n=20). After 8 weeks, the teeth were extracted and processed with hematoxylin-eosin staining for histologic evaluation. Most of the specimens in both groups developed a calcific barrier at the pulp amputation site and formed an odontoblast layer...
December 6, 2016: Dental Materials Journal
https://www.readbyqxmd.com/read/27927885/mesenchymal-cell-community-effect-in-whole-tooth-bioengineering
#12
L Yang, A Angelova Volponi, Y Pang, P T Sharpe
In vitro expanded cell populations can contribute to bioengineered tooth formation but only as cells that respond to tooth-inductive signals. Since the success of whole tooth bioengineering is predicated on the availability of large numbers of cells, in vitro cell expansion of tooth-inducing cell populations is an essential requirement for further development of this approach. We set out to investigate if the failure of cultured mesenchyme cells to form bioengineered teeth might be rescued by the presence of uncultured cells...
December 7, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27918898/roles-of-l-type-calcium-channels-cav1-2-and-the-distal-c-terminus-dct-in-differentiation-and-mineralization-of-rat-dental-apical-papilla-stem-cells-rscaps
#13
Qianqian Gao, Jianping Ge, Yanqin Ju, Changsheng Li, Jian Gao, Meisheng Wu, Shouliang Zhao
OBJECTIVE: Voltage-gated inward Ca(2+) currents (ICa) are triggered by cell depolarization and commonly produce transient increases in the cytoplasmic free Ca(2+) concentration. The CaV1.2 distal C-terminus is susceptible to proteolytic cleavage, which yields a truncated CaV1.2 subunit and a cleaved C-terminal fragment (CCt or DCT). Stem cells from the apical papilla (SCAPs) has a capacity for differentiation into the odontoblastic-like cells in vitro and dentin forming in vivo, which makes SCAPs advantages in tissue engineering and regenerative endodontic...
February 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/27905856/1-25-dihydroxyvitamin-d3-stimulates-odontoblastic-differentiation-of-human-dental-pulp-stem-cells-in-vitro
#14
Goksen Mucuk, Elif Sepet, Mine Erguven, Ozlem Ekmekcı, Ayhan Bılır
BACKGROUND: 1,25-Dihydroxyvitamin D3 (1,25-OH D3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). AIM: This study aimed to understand the influence of various concentrations of 1,25-OH D3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. MATERIALS AND METHODS: hDPSCs were obtained from the impacted third molar teeth...
December 1, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27866710/pulpal-tissue-inflammatory-reactions-after-experimental-pulpal-exposure-in-mice
#15
Ying He, Youhua Gan, Jiajian Lu, Qiong Feng, Haiyan Wang, Hongbing Guan, Qianzhou Jiang
INTRODUCTION: The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS: Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice were sacrificed randomly at 0, 1, 6, 12, 24, 48, and 72 hours after pulpal exposure. Mice without pulpal exposure served as controls. Maxillary teeth were obtained and prepared for histologic analyses and real-time polymerase chain reaction analyses...
January 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/27858058/-expression-of-opg-rank-rankl-in-the-rat-dental-pulp-tissue-of-periodontitis-combined-with-vascular-calcification-and-its-clinical-significance
#16
Ke-Qing Pan, Peng-Mei Zhang, Jing Deng, Xiu-Xiu Lou, Yun Meng, Gui-Rong Liu
PURPOSE: To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification. METHODS: Thirty-six male Wister rats were randomly divided into 4 groups: control group(group C), periodontitis group(group CP), vascular calcification group(group VDN) and compound group(group CP+VDN). Each group underwent corresponding management to establish animal model. When the model was successful, the maxillae including molars were sectioned, pulp tissue was examined by H-E staining; Immunohistochemical staining method was used to evaluate the expression and ratio of OPG and RANKL in pulp tissues...
August 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/27848099/dental-pulp-tissue-engineering-of-pulpotomized-rat-molars-with-bone-marrow-mesenchymal-stem-cells
#17
Takafumi Ito, Tomoatsu Kaneko, Yukiko Sueyama, Reika Kaneko, Takashi Okiji
The major goal of dental pulp tissue engineering is to enable the healing of inflamed tissue or to replace necrotic pulp tissue with newly formed dental pulp tissue. Here, we report a protocol for pulp tissue engineering in vivo in pulpotomized rat teeth using constructs of rat bone marrow mesenchymal stem cells, preformed biodegradable scaffolds, and hydrogel. The constructs were implanted into pulpotomized pulp chambers for 3, 7, or 14 days. At 3 days, cells were located mainly along the preformed scaffolds...
November 15, 2016: Odontology
https://www.readbyqxmd.com/read/27834664/%C3%AE-sma-expressing-perivascular-cells-represent-dental-pulp-progenitors-in-vivo
#18
I Vidovic, A Banerjee, R Fatahi, B G Matthews, N A Dyment, I Kalajzic, M Mina
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato(+) cells to a small number of newly formed odontoblasts during primary dentinogenesis...
November 10, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27805894/odontoblastic-cell-quantification-and-apoptosis-within-pulp-of-deciduous-teeth-versus-pulp-of-permanent-teeth
#19
Elena Bardellini, Francesca Amadori, Amerigo Santoro, Giulio Conti, Giovanna Orsini, Alessandra Majorana
OBJECTIVE: While the odontoblast ability to respond to injury in permanent teeth (PT) is well established, there is a lack of knowledge about deciduous teeth (DT). Aim of this study was to compare the odontoblasts activity within the pulp of DT versus the pulp of PT. STUDY DESIGN: Dental pulp was obtained from forty-two DT and twenty-seven PT extracted from sixty-five patients (aged 6-16 years). Histomorphometry was carried out and the quantification of odontoblastic layer was assessed...
2016: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/27793148/pdgfr%C3%AE-c-kit-pulp-cells-are-odontoblastic-progenitors-capable-of-producing-dentin-like-structure-in-vitro-and-in-vivo
#20
Shiwei Cai, Wenjian Zhang, Wei Chen
BACKGROUND: Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells responsive to PDGF and with dentin regeneration potential. METHODS: Pulp tissues were isolated from 12 freshly extracted human impacted third molars...
October 28, 2016: BMC Oral Health
keyword
keyword
72751
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"