keyword
MENU ▼
Read by QxMD icon Read
search

Odontoblast

keyword
https://www.readbyqxmd.com/read/28440310/klf5-mediates-odontoblastic-differentiation-through-regulating-dentin-specific-extracellular-matrix-gene-expression-during-mouse-tooth-development
#1
Zhuo Chen, Qi Zhang, Han Wang, Wentong Li, Feng Wang, Chunyan Wan, Shuli Deng, Hui Chen, Yixin Yin, Xiaoyan Li, Zhijian Xie, Shuo Chen
Klf5, a member of the Krüppel-like transcription factor family, has essential roles during embryonic development, cell proliferation, differentiation, migration and apoptosis. This study was to define molecular mechanism of Klf5 during the odontoblastic differentiation. The expression of Klf5, odontoblast-differentiation markers, Dspp and Dmp1 was co-localized in odontoblastic cells at different stages of mouse tooth development and mouse dental papilla mesenchymal cells. Klf5 was able to promote odontoblastic differentiation and enhance mineral formation of mouse dental papilla mesenchymal cells...
April 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28402144/expression-and-localization-of-yap-and-taz-during-development-of-the-mandibular-first-molar-in-rats
#2
B Zhang, B Y Sun, Y W Ji, Y P Zhang, X X Wang, X Xu, Y Wen
Yes-associated protein (Yap) and transcriptional coactivator with PDZ-binding motif (Taz) are two downstream factors in the Hippo signaling pathway. Yap and Taz participate in regulating organ size, stem cell self-renewal, proliferation and differentiation. We investigated the spatial-temporal expression and relative expression levels of Yap and Taz using immunohistochemistry and real-time polymerase chain reaction. We found Yap and Taz in the oral epithelium and mesenchyme at embryonic (E) day 14.5 (E14.5) and E16...
2017: Biotechnic & Histochemistry: Official Publication of the Biological Stain Commission
https://www.readbyqxmd.com/read/28384982/amelogenin-gene-the-pioneer-in-gender-determination-from-forensic-dental-samples
#3
Priyanjali Dutta, Satish Bhosale, Rajeshwar Singh, Priyanka Gubrellay, Jitendra Patil, Bhumika Sehdev, Sachin Bhagat, Tajinder Bansal
INTRODUCTION: In the event of any mass fatality incident, DNA analysis plays a vital role in disaster victim identification. Teeth are one of the most resistant structures in the human body that resist decomposition hence making them prime choice for extracting DNA for identification of individuals. Polymerase Chain Reaction (PCR) analysis that target regions of Amelogenin gene have become the method of choice for sex determination of biological samples. AIM: Determining the sex of a given DNA sample from either dental pulp or dentin of tooth and help in identification of missing persons and disaster victims...
February 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28380697/graphene-oxide-enrichment-collagen-membranes-improves-dpscs-differentiation-and-controls-inflammation-occurrence
#4
Milena Radunovic, Marianna De Colli, Patrizia De Marco, Chiara Di Nisio, Antonella Fontana, Adriano Piattelli, Amelia Cataldi, Susi Zara
Collagen membranes are used in oral surgery for bone defects treatment acting as a barrier that does not allow the invasion of soft tissue into the growing bone. To improve biocompatibility collagen membranes were coated with graphene oxide (GO), a graphene derivative. The aim of this study was to investigate the biocompatibility of GO coated collagen membranes on human dental pulp stem cells (DPSCs) focusing on biomaterial cytotoxicity, ability to promote DPSCs differentiation process and to control inflammation event induction...
April 5, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28371626/correlation-between-numbers-of-cells-in-human-dental-pulp-and-age-implications-for-age-estimation
#5
Mohammad Zakir Hossain, Sulinda Daud, Phrabhakaran Nambiar, Fathilah Abdul Razak, Norintan Ab-Murat, Roslan Saub, Marina M Bakri
OBJECTIVE: The aim of this study was to investigate correlations between dental pulp cell count of odontoblasts, subodontoblasts and fibroblasts and age, within different age groups. Formulation of regression equations using the dental pulp cell count for predicting age was attempted. DESIGN: Eighty-one extracted teeth were grouped into two age groups (6-25 years, 26-80 years). The teeth were demineralized and histological sections were prepared for cell count. Regression equations were generated from regression analysis of cell count and tested for age estimation...
March 24, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28365680/pro-inflammatory-cytokine-tnf-%C3%AE-attenuates-bmp9-induced-osteo-odontoblastic-differentiation-of-the-stem-cells-of-dental-apical-papilla-scaps
#6
Feilong Wang, Ying Jiang, Xia Huang, Qiao Liu, Yan Zhang, Wenping Luo, Fugui Zhang, Pengfei Zhou, Juhong Lin, Hongmei Zhang
BACKGROUND/AIMS: Periapical periodontitis is a common oral disease caused by bacterial invasion of the tooth pulp, which usually leads to local release of pro-inflammatory cytokines and osteolytic lesion. This study is intended to examine the effect of TNF-α on BMP9-induced osteogenic differentiation of the stem cells of dental apical papilla (SCAPs). METHODS: Rat model of periapical periodontitis was established. TNF-α expression was assessed. Osteogenic markers and ectopic bone formation in iSCAPs were analyzed upon BMP9 and TNF-α treatment...
March 31, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28355287/the-sirt6-gene-does-it-play-a-role-in-tooth-development
#7
Xueyang Liao, Bo Feng, Demao Zhang, Peng Liu, Xuedong Zhou, Ruimin Li, Ling Ye
Dental Mesenchymal Cells (DMCs) are known to play a role in tooth development as well as in the repair and regeneration of dental tissue. A large number of signaling molecules regulate the proliferation and differentiation of DMC, though the underlying mechanisms are still not fully understood. Sirtuin-6 (SIRT6), a key regulator of aging, can exert an impact on embryonic stem cell (ESC) differentiation. The experimental deletion of Sirt6 in mouse bone marrow cells has been found to have an inhibiting impact on the bone mineral density and the osteogenic differentiation of these cells...
2017: PloS One
https://www.readbyqxmd.com/read/28333374/odontoclastogenesis-of-mouse-papilla-derived-mdpc-23-cells-induced-by-lps
#8
C Li, W T Qi, H W Jiang
AIM: To investigate the role of LPS in the odontoclast differentiation of MDPC-23 cells. It was hypothesized that MDPC 23 odontoblast-like cells may function as odontoclasts under the influence of LPS. METHODOLOGY: MDPC-23 cells were cultured in the presence of 0.1 or 1 μg/mL LPS for 6 days. Cell viability was determined using the CCK8 assay. TRAP staining, dentine resorption assay and ROS detection by confocal laser scanning microscope were used to test the odontoclast-like function of the induced cells...
March 23, 2017: International Endodontic Journal
https://www.readbyqxmd.com/read/28331230/dentin-sialoprotein-facilitates-dental-mesenchymal-cell-differentiation-and-dentin-formation
#9
Wentong Li, Lei Chen, Zhuo Chen, Lian Wu, Junsheng Feng, Feng Wang, Lisa Shoff, Xin Li, Kevin J Donly, Mary MacDougall, Shuo Chen
Dentin sialoprotein (DSP) is a dentin extracellular matrix protein. It is involved in dental mesenchymal cell lineages and dentin formation through regulation of its target gene expression. DSP mutations cause dentin genetic diseases. However, mechanisms of DSP in controlling dental mesenchymal cell differentiation are unknown. Using DSP as bait, we screened a protein library from mouse odontoblastic cells and found that DSP is a ligand and binds to cell surface receptor, occludin. Further study identified that the C-terminal DSP domain(aa 363-458) interacts with the occludin extracellular loop 2(aa 194-241)...
March 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28316916/human-ex-vivo-dentin-pulp-complex-preservation-in-a-full-crown-model
#10
João Botelho, Maria Alzira Cavacas, Gonçalo Borrecho, Mário Polido, Pedro Oliveira, José Martins Dos Santos
OBJECTIVES: Currently, there is lack of human in vitro full tooth models that hold the odontoblast layer with pulp tissue in their native environment. The appearance of new in vitro and in vivo models has provided new understanding of the potential of tissue engineering in dental pulp regeneration. However, the development of new in vitro full tooth models will allow us to get closer to in vivo conditions. Thus, the aim of this study is to preserve a living dentin-pulp complex, in a novel in vitro full crown model, after tooth extraction...
January 2017: Journal of Oral Biology and Craniofacial Research
https://www.readbyqxmd.com/read/28301019/cytotoxicity-of-new-calcium-aluminate-cement-endobinder-containing-different-radiopacifiers
#11
Claudia Huck, Hernane da Silva Barud, Fernanda Gonçalves Basso, Carlos Alberto de Souza Costa, Josimeri Hebling, Lucas da Fonseca Roberti Garcia
This study aimed to evaluate the cytotoxicity of a calcium aluminate cement (EndoBinder) containing different radiopacifiers, Bi2O3, ZnO or ZrO2, compared with Mineral Trioxide Aggregate (MTA). According to ISO 10993-12:2012 (E) recommendations, 0.2 g of each cement were applied in transwell inserts and placed in 24-well culture plates containing 1 mL of culture medium (DMEM). After 24 h of incubation, the extracts (DMEM containing components released from the cements) were applied to immortalized odontoblast-like MDPC-23 cells...
January 2017: Brazilian Dental Journal
https://www.readbyqxmd.com/read/28292713/transforming-growth-factor-%C3%AE-induced-gene-product-h3-inhibits-odontoblastic-differentiation-of-dental-pulp-cells
#12
Suguru Serita, Atsushi Tomokiyo, Daigaku Hasegawa, Sayuri Hamano, Hideki Sugii, Shinichiro Yoshida, Hiroyuki Mizumachi, Hiromi Mitarai, Satoshi Monnouchi, Naohisa Wada, Hidefumi Maeda
OBJECTIVE: The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). DESIGN: A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining...
February 24, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28281011/odontogenic-differentiation-potential-of-human-dental-pulp-cells-cultured-on-a-calcium-aluminate-enriched-chitosan-collagen-scaffold
#13
Diana Gabriela Soares, Hebert Luís Rosseto, Débora Salles Scheffel, Fernanda Gonçalves Basso, Claudia Huck, Josimeri Hebling, Carlos Alberto de Souza Costa
OBJECTIVE: The study aims to evaluate the odontogenic potential of human dental pulp cells (HDPCs) in contact with an experimental porous chitosan-collagen scaffold (CHC) enriched or not with a mineral phase of calcium-aluminate (CHC-CA). MATERIAL AND METHODS: To assess the chemotactic effect of the materials, we placed HDPCs seeded on transwell membranes in intimate contact with the CHC or CHC-CA surface, and the cell migration was monitored for 48 h. Additionally, cells were seeded onto the material surface, and the viability and proliferation were evaluated at several time points...
March 9, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28253279/demineralized-bone-matrix-used-for-direct-pulp-capping-in-rats
#14
Qian Liu, Yanhong Ma, Junlan Wang, Xuefang Zhu, Yanjing Yang, Yufeng Mei
OBJECTIVES: To evaluate the wound healing process following direct pulp capping with demineralized bone matrix (DBM) and calcium hydroxide (Ca(OH)2). METHODS: Fifty 8-weeks-old SPF Wistar male rats were divided into two groups: one was the DBM treated group, and the other was the Ca(OH)2 treated group. Pulpotomy was performed on the maxillary first molar of one side of each rat, and the another side was left as the blank control. Rats were sacrificed after each observation period (1, 3, 7, 14 and 28 days) and specimen slices were made...
2017: PloS One
https://www.readbyqxmd.com/read/28239033/hertwig-s-epithelial-root-sheath-cells-contribute-to-formation-of-periodontal-ligament-through-epithelial-mesenchymal-transition-by-tgf-%C3%AE
#15
Satoshi Itaya, Kyoko Oka, Kayoko Ogata, Shougo Tamura, Michiko Kira-Tatsuoka, Naoki Fujiwara, Keishi Otsu, Eichi Tsuruga, Masao Ozaki, Hidemitsu Harada
In tooth root development, periodontal ligament (PDL) and cementum are formed by the coordination with the fragmentation of Hertwig's epithelial root sheath (HERS) and the differentiation of dental follicle mesenchymal cells. However, the function of the dental epithelial cells after HERS fragmentation in the PDL is not fully understood. Here, we found that TGF-β regulated HERS fragmentation via epithelial-mesenchymal transition (EMT), and the fragmented epithelial cells differentiated into PDL fibroblastic cells with expressing of PDL extracellular matrix (ECM)...
2017: Biomedical Research
https://www.readbyqxmd.com/read/28238937/sp1-is-a-competitive-endogenous-rna-of-klf4-during-odontoblast-differentiation
#16
Jie Zhang, Huan Liu, Heng Lin, Shuchen Li, Huangheng Tao, Lu Zhang, Guohua Yuan, Zhi Chen
Our previous studies have demonstrated that KLF4 is a critical transcription factor that promotes the odontoblastic differentiation of dental papilla cells. Klf4 mRNA was found to be regulated by multiple microRNAs (miRNAs). Competitive endogenous RNAs (ceRNAs) are a group of transcripts post-transcriptionally regulating each other by competing for their common miRNAs. However, the regulation of Klf4 by ceRNAs in odontoblastic differentiation remains unknown. In this study, we predicted a group of potential Klf4 ceRNAs with bioinformatics approach, and examined the expression of Klf4 and five interested potential ceRNAs including Sp1 using real-time PCR during odontoblastic differentiation of mDPC6T...
February 24, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28222099/morphological-study-of-tooth-development-in-podoplanin-deficient-mice
#17
Kenyo Takara, Naoki Maruo, Kyoko Oka, Chiaki Kaji, Yuji Hatakeyama, Naruhiko Sawa, Yukinari Kato, Junro Yamashita, Hiroshi Kojima, Yoshihiko Sawa
Podoplanin is a mucin-type highly O-glycosylated glycoprotein identified in several somatyic cells: podocytes, alveolar epithelial cells, lymphatic endothelial cells, lymph node stromal fibroblastic reticular cells, osteocytes, odontoblasts, mesothelial cells, glia cells, and others. It has been reported that podoplanin-RhoA interaction induces cytoskeleton relaxation and cell process stretching in fibroblastic cells and osteocytes, and that podoplanin plays a critical role in type I alveolar cell differentiation...
2017: PloS One
https://www.readbyqxmd.com/read/28221099/sphingomyelin-phosphodiesterase-3-enhances-cytodifferentiation-of-periodontal-ligament-cells
#18
S Miyauchi, J Kitagaki, R Masumoto, A Imai, K Kobayashi, A Nakaya, S Kawai, C Fujihara, Y Asano, M Yamashita, M Yanagita, S Yamada, M Kitamura, S Murakami
Sphingomyelin phosphodiesterase 3 ( Smpd3), which encodes neutral sphingomyelinase 2 (nSMase2), is a key molecule for skeletal development as well as for the cytodifferentiation of odontoblasts and alveolar bone. However, the effects of nSMase2 on the cytodifferentiation of periodontal ligament (PDL) cells are still unclear. In this study, the authors analyzed the effects of Smpd3 on the cytodifferentiation of human PDL (HPDL) cells. The authors found that Smpd3 increases the mRNA expression of calcification-related genes, such as alkaline phosphatase (ALPase), type I collagen, osteopontin, Osterix (Osx), and runt-related transcription factor (Runx)-2 in HPDL cells...
March 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28215344/rac1-regulates-the-proliferation-adhesion-migration-and-differentiation-of-mdpc-23-cells
#19
Jing Ren, Guobin Liang, Li Gong, Bing Guo, Hongwei Jiang
INTRODUCTION: Stem cells are responsible for replacing damaged pulp tissue; therefore, promoting their survival and inducing their adhesion to dentin are vital. As a member of the Rho family of guanosine triphosphatases, Rac1 is an important regulator of osteoblast functions. However, little is known about its role in regenerative endodontic procedures. The current study examined the role of Rac1 in the proliferation, migration, and odontoblastic differentiation of MDPC-23 cells. METHODS: MDPC-23 cells were transfected with small interfering RNA to knock down Rac1 expression, and then their proliferation, migration, adhesion, and odontoblastic differentiation were examined in vitro...
April 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28211068/dentine-matrix-proteins-isolation-and-effects-on-human-pulp-cells
#20
M Widbiller, A Eidt, S R Lindner, K-A Hiller, H Schweikl, W Buchalla, K M Galler
AIM: To establish a simplified and efficient protocol for the isolation and concentration of matrix proteins from human dentine, and to assess the effects of extracted dentine matrix proteins (eDMP) on the behavior of human pulp cells. METHODOLOGY: Matrix proteins were isolated from human dentine, purified, concentrated and characterized with protein and enzyme-linked immunosorbent assays. Culture media were supplemented with eDMP in different concentrations, referred to as eDMP 1 to 10,000, to assess viability and proliferation of human pulp cells by DNA and MTT assays; apoptotic events were quantified by flow cytometry...
February 17, 2017: International Endodontic Journal
keyword
keyword
72751
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"