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https://www.readbyqxmd.com/read/29765331/high-ph-sensitive-store-operated-ca-2-entry-mediated-by-ca-2-release-activated-ca-2-channels-in-rat-odontoblasts
#1
Maki Kimura, Koichi Nishi, Asuka Higashikawa, Sadao Ohyama, Kaoru Sakurai, Masakazu Tazaki, Yoshiyuki Shibukawa
Odontoblasts play a crucial role in dentin formation and sensory transduction following the application of stimuli to the dentin surface. Various exogenous and endogenous stimuli elicit an increase in the intracellular free calcium concentration ([Ca2+ ]i ) in odontoblasts, which is mediated by Ca2+ release from intracellular Ca2+ stores and/or Ca2+ influx from the extracellular medium. In a previous study, we demonstrated that the depletion of Ca2+ stores in odontoblasts activated store-operated Ca2+ entry (SOCE), a Ca2+ influx pathway...
2018: Frontiers in Physiology
https://www.readbyqxmd.com/read/29752081/effect-of-polyhydroxybutyrate-chitosan-bioglass-nanofiber-scaffold-on-proliferation-and-differentiation-of-stem-cells-from-human-exfoliated-deciduous-teeth-into-odontoblast-like-cells
#2
Maryam Khoroushi, Mohammad Reza Foroughi, Saeed Karbasi, Batool Hashemibeni, Abbas Ali Khademi
Scaffolds and their characteristics play a central role in tissue engineering. The purpose of this study was to determine the effects of Polyhydroxybutyrate (PHB)/Chitosan/nano-bioglass (nBG) nanofiber scaffold made using the electrospinning method, on the proliferation and differentiation of stem cells obtained from human exfoliated deciduous teeth into odontoblast-like cells. In this experimental study, the pulps of the molten deciduous teeth were isolated, thereafter, the stem cells from human exfoliated deciduous teeth (SHED) were extracted and then the 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the cell viability percentage...
August 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/29745817/impaired-mineral-quality-in-dentin-in-x-linked-hypophosphatemia
#3
Benjamin R Coyac, Guillaume Falgayrac, Guillaume Penel, Alain Schmitt, Thorsten Schinke, Agnès Linglart, Marc D McKee, Catherine Chaussain, Claire Bardet
X-linked hypophosphatemia (XLH) is a skeletal disorder arising from mutations in the PHEX gene, transmitted in most cases as an X-linked dominant trait. PHEX deficiency leads to renal phosphate wasting and hypophosphatemia, as well as impaired mineralization of bone and dentin, resulting in severe skeletal and dental complications. Dentin mineralization defects appear as characteristic, large interglobular spaces resulting from the lack of fusion of calculospherites in the circumpulpal region during the mineralization process...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745814/trip-1-in-the-extracellular-matrix-promotes-nucleation-of-calcium-phosphate-polymorphs
#4
Amsaveni Ramachandran, Kun He, Chun-Chieh Huang, Reza Shahbazian-Yassar, Tolou Shokuhfar, Anne George
In search for bone and dentin extracellular matrix (ECM) proteins, transforming growth factor beta receptor II interacting protein 1 (TRIP-1) was identified as a novel protein synthesized by osteoblasts and odontoblasts and exported to the ECM. TRIP-1 is a WD-40 (WD is Tryptophan-Aspartic acid dipeptide) protein that has been well recognized for its physiological role in the endoplasmic reticulum (ER). In the ER, TRIP-1 functions as an essential subunit of eukaryotic elongation initiation factor 3 and is involved in the protein translational machinery...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29745808/the-er-ca-2-sensor-stim1-can-activate-osteoblast-and-odontoblast-differentiation-in-mineralized-tissues
#5
Yinghua Chen, Amsaveni Ramachandran, Youbin Zhang, Rahul Koshy, Anne George
Bone and dentin development requires temporal and spatial deposition of calcium phosphate mineral. A host of proteins works in concert to contribute to this tightly regulated process while malfunction in this scheme often leads to pathological defects. We have reported earlier that DMP1 stimulation of preosteoblasts leads to calcium release from internal Ca2+ stores and this store depletion is sensed by the ER Ca2+ sensor STIM1 (stromal interaction molecule 1). In this study, we first assessed the temporal and spatial localization of STIM1 protein during the development of bone and dentin by immunohistochemical methods...
December 2018: Connective Tissue Research
https://www.readbyqxmd.com/read/29741358/fibrous-topography-potentiated-canonical-wnt-signaling-directs-the-odontoblastic-differentiation-of-dental-pulp-derived-stem-cells
#6
Saeed Ur Rahman, Joung-Hwan Oh, Young-Dan Cho, Shin Hye Chung, Gene Lee, Jeong-Hwa Baek, Hyun-Mo Ryoo, Kyung Mi Woo
Nanofibrous engineered matrices have significant potential in cellular differentiation and tissue regeneration. Stem cells require specific extracellular signals that lead to the induction of different lineages. However, the mechanisms by which the nanofibrous matrix promotes mesenchymal stem cell (MSC) differentiation are largely unknown. Here, we investigated the mechanisms which underlie nanofibrous matrix-induced odontoblastic differentiation of human dental pulp MSCs (DP-MSCs). An electrospun polystyrene nanofibrous (PSF) matrix was prepared, and the cell responses to the PSF matrix were assessed in comparison with those on conventional tissue culture dishes...
May 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29731830/rho-rho-associated-protein-kinase-signaling-pathway-mediated-downregulation-of-runt-related-transcription-factor-2-expression-promotes-the-differentiation-of-dental-pulp-stem-cells-into-odontoblasts
#7
Xiaoqing Huang, Xiaoling Chen, Hongbai Chen, Dongwei Xu, Chen Lin, Bin Peng
The present study investigated the role of runt-related transcription factor 2 (Runx2) in regulating the differentiation of human dental pulp stem cells (hDPSCs) into odontoblasts under the mediation of the Rho/Rho-associated protein kinase (ROCK) signaling pathway. hDPSCs and human bone marrow mesenchymal stem cells (hBMSCs) were mineralized to induce differentiation. The expression levels of odontoblast- and osteoblast-specific proteins, dentin sialophosphoprotein (DSPP), osteocalcin (OCN) and Runx2, were measured using western blot analysis...
May 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29709295/activation-of-mechanosensitive-transient-receptor-potential-piezo-channels-in-odontoblasts-generates-action-potentials-in-cocultured-isolectin-b-4-negative-medium-sized-trigeminal-ganglion-neurons
#8
Masaki Sato, Kazuhiro Ogura, Maki Kimura, Koichi Nishi, Masayuki Ando, Masakazu Tazaki, Yoshiyuki Shibukawa
INTRODUCTION: Various stimuli to the dentin surface elicit dentinal pain by inducing dentinal fluid movement causing cellular deformation in odontoblasts. Although odontoblasts detect deformation by the activation of mechanosensitive ionic channels, it is still unclear whether odontoblasts are capable of establishing neurotransmission with myelinated A delta (Aδ) neurons. Additionally, it is still unclear whether these neurons evoke action potentials by neurotransmitters from odontoblasts to mediate sensory transduction in dentin...
April 27, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29707538/insulin-like-growth-factor-axis-expression-in-dental-pulp-cells-derived-from-carious-teeth
#9
Hanaa Esa Alkharobi, Hasanain Al-Khafaji, James Beattie, Deirdre Ann Devine, Reem El-Gendy
The insulin-like growth factor (IGF) axis plays an important role in dental tissue regeneration and most components of this axis are expressed in human dental pulp cells (DPCs). In our previous study, we analyzed IGF axis gene expression in DPCs and demonstrated a novel role of IGF binding protein (IGFBP)-2 and -3 in coordinating mineralized matrix formation in differentiating DPCs. A more recent study from our laboratory partially characterized dental pulp stem cells from teeth with superficial caries (cDPCs) and showed that their potential to differentiate odontoblasts and/or into osteoblasts is enhanced by exposure to the mild inflammatory conditions characteristic of superficial caries...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29703620/a-comparative-evaluation-of-concentrated-growth-factor-and-platelet-rich-fibrin-on-the-proliferation-migration-and-differentiation-of-human-stem-cells-of-the-apical-papilla
#10
Shebin Hong, Weiting Chen, Beizhan Jiang
INTRODUCTION: Concentrated growth factor (CGF) is considered to be a natural biomaterial that is better than platelet-rich fibrin (PRF) in bone regeneration, but there is little information acquired in regenerative endodontics. Therefore, the purpose of this study was to evaluate their effects on the proliferation, migration, and differentiation of human stem cells of the apical papilla (SCAPs). METHODS: CGF- and PRF-conditioned medium were prepared using the freeze-dried method...
April 24, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29703618/biological-analysis-of-simvastatin-releasing-chitosan-scaffold-as-a-cell-free-system-for-pulp-dentin-regeneration
#11
Diana G Soares, Giovanna Anovazzi, Ester Alves F Bordini, Uxua O Zuta, Maria Luísa A Silva Leite, Fernanda G Basso, Josimeri Hebling, Carlos A de Souza Costa
INTRODUCTION: The improvement of biomaterials capable of driving the regeneration of the pulp-dentin complex mediated by resident cells is the goal of regenerative dentistry. In the present investigation, a chitosan scaffold (CHSC) that released bioactive concentrations of simvastatin (SIM) was tested, aimed at the development of a cell-free tissue engineering system. METHODS: First, we performed a dose-response assay to select the bioactive dose of SIM capable of inducing an odontoblastic phenotype in dental pulp cells (DPCs); after which we evaluated the synergistic effect of this dosage with the CHSC/DPC construct...
April 24, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29702010/pulp-regeneration-by-3-dimensional-dental-pulp-stem-cell-constructs
#12
Y Itoh, J I Sasaki, M Hashimoto, C Katata, M Hayashi, S Imazato
Dental pulp regeneration therapy for the pulpless tooth has attracted recent attention, and clinical trial studies are underway with the tissue engineering approach. However, there remain many concerns, including the extended period for regenerating the dental pulp. In addition, the use of scaffolds increases the risk of inflammation and infection. To establish a basic technology for novel dental pulp regenerative therapy that allows transplant of pulp-like tissue, we attempted to fabricate scaffold-free 3-dimensional (3D) cell constructs composed of dental pulp stem cells (DPSCs)...
April 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29697396/influence-of-tricalcium-silicate-on-course-of-traumatic-pulpitis
#13
I Kovach, K Buniatian, A Makarevych, A Verbyts'ka, V Gargin
The use of Tricalcium Silicate (TS) as an odontotropic preparation makes it possible to create a hermetic crown restoration with a high degree of adhesion. However, the use of TS silicate by direct pulp capping remains disputable. The aim of this study was to determine the effects of TS on course of traumatic pulpitis by detection of morpho-functional peculiarities of changes in pulp tissue. We performed experimental investigation (on rabbits, males, aging three-month) for study of the morpho-functional changes of the pulp tissues with modeling of traumatic pulpitis and direct pulp capping with TS preparation (8 animals, investigated group) and calcium hydroxide (Calasept, NORDISKA DENTAL) preparation (8 animals, comparison group)...
March 2018: Georgian Medical News
https://www.readbyqxmd.com/read/29691549/-expression-pattern-of-kr%C3%A3-pple-like-factor-5-in-odontoblasts
#14
Jian-Xin DU, Wei Hu
PURPOSE: To investigate the expression of Krüpple-like factor 5 (KLF5) in human pulp-dentin complex and the function of KLF5 in differentiation and dentinogenesis of odontoblasts. METHODS: Immunohistochemistry staining was used to investigate the expression of KLF5 in human pulp-dentin complex and dental pulp cells. Western blot was used to analyze the expression patterns of KLF5 during dental pulp cell differentiation. Significant differences between groups were evaluated using one-way ANOVA and Tukey's test with SPSS17...
December 2017: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/29680724/human-dental-pulp-cells-express-cellular-markers-for-inflammation-and-hard-tissue-formation-in-response-to-bacterial-information
#15
Akina Sugiuchi, Yousuke Sano, Masahiro Furusawa, Shu Abe, Takashi Muramatsu
INTRODUCTION: Lipopolysaccharide (LPS) is a major component of the outer membranes of gram-negative bacteria associated with deep dental caries and pulpitis. When bacteria invade dentinal tubes and dentin is continually destroyed, tertiary dentin is formed by preexisting odontoblasts. However, the relationship between LPS and tertiary dentin formation remains unclear. We investigated whether LPS stimulation induces the formation of hard tissue in human dental pulp cells (hDPCs). METHODS: Immortalized hDPCs were cultured, and Escherichia coli-derived LPS (1 μg/mL) was incorporated into the culture medium...
April 18, 2018: Journal of Endodontics
https://www.readbyqxmd.com/read/29679496/detection-of-bone-marrow-derived-fibrocytes-in-human-dental-pulp-repair
#16
N Yoshiba, N Edanami, A Tohma, R Takeuchi, N Ohkura, A Hosoya, Y Noiri, H Nakamura, K Yoshiba
AIM: To explore the expression profile of CD45+/pro-collagen I+ fibrocytes in intact dental pulps as well as during wound healing in adult dental pulp tissue. METHODOLOGY: A total of 16 healthy permanent teeth were obtained from young patients (18 to 25 years) undergoing orthodontic treatment. Routine pulp capping with mineral trioxide aggregate (MTA) was performed under local anaesthesia to induce a mineralized barrier at the exposed surface. Teeth were extracted from patients after 7, 14, and 35 days...
April 21, 2018: International Endodontic Journal
https://www.readbyqxmd.com/read/29675422/elucidation-on-predominant-pathways-involved-in-the-differentiation-and-mineralization-of-odontoblast-like-cells-by-selective-blockade-of-mitogen-activated-protein-kinases
#17
Jia Tang, Takashi Saito
Aim: To analyze the effect of three mitogen-activated protein kinase (MAPK) inhibitors, namely, SB202190 (p38 inhibitor), SP600125 (JNK inhibitor), and PD98059 (ERK inhibitor) in Dex-stimulated MDPC-23 cell differentiation and mineralization. Methods: Experiment was divided into five groups, control (cells without Dex and inhibitors treatment), Dex (cells with Dex treatment but without inhibitors), Dex + SB202190, Dex + SP600125, and Dex + PD98059. Cell differentiation was assessed by alkaline phosphatase (ALP) activity assay and real time RT-PCR...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29670655/odontoblast-like-cells-differentiated-from-dental-pulp-stem-cells-retain-their-phenotype-after-subcultivation
#18
Paula A Baldión, Myriam L Velandia-Romero, Jaime E Castellanos
Odontoblasts, the main cell type in teeth pulp tissue, are not cultivable and they are responsible for the first line of response after dental restauration. Studies on dental materials cytotoxicity and odontoblast cells physiology require large quantity of homogenous cells retaining most of the phenotype characteristics. Odontoblast-like cells (OLC) were differentiated from human dental pulp stem cells using differentiation medium (containing TGF- β 1), and OLC expanded after trypsinization (EXP-21) were evaluated and compared...
2018: International Journal of Cell Biology
https://www.readbyqxmd.com/read/29663824/immunohistochemical-study-on-antigen-presenting-cells-in-healthy-and-carious-human-teeth
#19
A Sotirovska Ivkovska, E Zabokova-Bilbilova, Z Georgiev, E Bajraktarova Valjakova, L Ivkovski
Class II major histocompatibility complex (MHC) antigen-presenting cells are associated with the early phase of the immune response. We have studied the distribution of class II-expressing cells in developing, healthy and carious human teeth to clarify, when human pulp acquires an immunologic defense potential and how this reacts to dental caries. Antigen-expressing cells were identified immunohistochemically with the following monoclonal antibodies: HLA-DR - for dendritic cells and CD68 - for macrophages. In the pulp of unerupted developing teeth, HLA-DR-positive cells were distributed mainly in and around the odontoblast layer...
2018: Bratislavské Lekárske Listy
https://www.readbyqxmd.com/read/29663385/quaking-promotes-the-odontoblastic-differentiation-of-human-dental-pulp-stem-cells
#20
Shuchen Li, Chujiao Lin, Jie Zhang, Huangheng Tao, Huan Liu, Guohua Yuan, Zhi Chen
Odontoblastic differentiation of human dental pulp stem cells (hDPSCs) is essential for the formation of reparative dentin after dental caries or injury. Our previous studies have demonstrated that krüppel-like factor 4 (KLF4) is a critical transcription factor that promotes the odontoblastic differentiation of hDPSCs. Analysis of the microRNA binding sites within the 3'-UTR of KLF4 revealed that QKI, an RNA-binding protein, shared the most microRNAs with KLF4, presumably served as a "competent endogenous RNA (ceRNA)" with KLF4...
April 16, 2018: Journal of Cellular Physiology
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