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https://www.readbyqxmd.com/read/27905856/1-25-dihydroxyvitamin-d3-stimulates-odontoblastic-differentiation-of-human-dental-pulp-stem-cells-in-vitro
#1
Goksen Mucuk, Elif Sepet, Mine Erguven, Ozlem Ekmekci, Ayhan Bilir
BACKGROUND: 1,25-dihydroxyvitamin D3 (1,25-OH D3) plays an important role in mineralized tissue metabolism, including teeth. However, few studies have addressed its role in odontoblastic differentiation of human dental pulp-stem cells (hDPSCs). AIM: This study aimed to understand the influence of various concentrations of 1,25-OH D3 on the proliferation capacity and early dentinogenesis responses of hDPSCs. MATERIALS AND METHODS: hDPSCs were obtained from the impacted third molar teeth...
December 1, 2016: Connective Tissue Research
https://www.readbyqxmd.com/read/27866710/pulpal-tissue-inflammatory-reactions-after-experimental-pulpal-exposure-in-mice
#2
Ying He, Youhua Gan, Jiajian Lu, Qiong Feng, Haiyan Wang, Hongbing Guan, Qianzhou Jiang
INTRODUCTION: The purpose of this study was to establish a stable experimental mice pulpal inflammatory model and to evaluate inflammatory reactions of pulpal tissue after pulpal exposure. METHODS: Pulpal inflammation was induced in 80 C57BL/6 mice by occlusal exposure of the pulp of the maxillary first molar. The mice were sacrificed randomly at 0, 1, 6, 12, 24, 48, and 72 hours after pulpal exposure. Mice without pulpal exposure served as controls. Maxillary teeth were obtained and prepared for histologic analyses and real-time polymerase chain reaction analyses...
November 17, 2016: Journal of Endodontics
https://www.readbyqxmd.com/read/27858058/-expression-of-opg-rank-rankl-in-the-rat-dental-pulp-tissue-of-periodontitis-combined-with-vascular-calcification-and-its-clinical-significance
#3
Ke-Qing Pan, Peng-Mei Zhang, Jing Deng, Xiu-Xiu Lou, Yun Meng, Gui-Rong Liu
PURPOSE: To study the expression and possible role of OPG/RANK/RANKLin the rat dental pulp of periodontitis combined with vascular calcification. METHODS: Thirty-six male Wister rats were randomly divided into 4 groups: control group(group C), periodontitis group(group CP), vascular calcification group(group VDN) and compound group(group CP+VDN). Each group underwent corresponding management to establish animal model. When the model was successful, the maxillae including molars were sectioned, pulp tissue was examined by H-E staining; Immunohistochemical staining method was used to evaluate the expression and ratio of OPG and RANKL in pulp tissues...
August 2016: Shanghai Kou Qiang Yi Xue, Shanghai Journal of Stomatology
https://www.readbyqxmd.com/read/27848099/dental-pulp-tissue-engineering-of-pulpotomized-rat-molars-with-bone-marrow-mesenchymal-stem-cells
#4
Takafumi Ito, Tomoatsu Kaneko, Yukiko Sueyama, Reika Kaneko, Takashi Okiji
The major goal of dental pulp tissue engineering is to enable the healing of inflamed tissue or to replace necrotic pulp tissue with newly formed dental pulp tissue. Here, we report a protocol for pulp tissue engineering in vivo in pulpotomized rat teeth using constructs of rat bone marrow mesenchymal stem cells, preformed biodegradable scaffolds, and hydrogel. The constructs were implanted into pulpotomized pulp chambers for 3, 7, or 14 days. At 3 days, cells were located mainly along the preformed scaffolds...
November 15, 2016: Odontology
https://www.readbyqxmd.com/read/27834664/%C3%AE-sma-expressing-perivascular-cells-represent-dental-pulp-progenitors-in-vivo
#5
I Vidovic, A Banerjee, R Fatahi, B G Matthews, N A Dyment, I Kalajzic, M Mina
The goal of this study was to examine the contribution of perivascular cells to odontoblasts during the development, growth, and repair of dentin using mouse molars as a model. We used an inducible, Cre-loxP in vivo fate-mapping approach to examine the contributions of the descendants of cells expressing the αSMA-CreERT2 transgene to the odontoblast lineage. In vivo lineage-tracing experiments in molars showed the contribution of αSMA-tdTomato(+) cells to a small number of newly formed odontoblasts during primary dentinogenesis...
November 10, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27805894/odontoblastic-cell-quantification-and-apoptosis-within-pulp-of-deciduous-teeth-versus-pulp-of-permanent-teeth
#6
Elena Bardellini, Francesca Amadori, Amerigo Santoro, Giulio Conti, Giovanna Orsini, Alessandra Majorana
OBJECTIVE: While the odontoblast ability to respond to injury in permanent teeth (PT) is well established, there is a lack of knowledge about deciduous teeth (DT). Aim of this study was to compare the odontoblasts activity within the pulp of DT versus the pulp of PT. STUDY DESIGN: Dental pulp was obtained from forty-two DT and twenty-seven PT extracted from sixty-five patients (aged 6-16 years). Histomorphometry was carried out and the quantification of odontoblastic layer was assessed...
2016: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/27793148/pdgfr%C3%AE-c-kit-pulp-cells-are-odontoblastic-progenitors-capable-of-producing-dentin-like-structure-in-vitro-and-in-vivo
#7
Shiwei Cai, Wenjian Zhang, Wei Chen
BACKGROUND: Successful pulp regeneration depends on identification of pulp stem cells capable of differentiation under odontoblastic lineage and producing pulp-dentinal like structure. Recent studies demonstrate that platelet-derived growth factor (PDGF) plays an important role in damage repair and tissue regeneration. The aim of this study was to identify a subpopulation of dental pulp cells responsive to PDGF and with dentin regeneration potential. METHODS: Pulp tissues were isolated from 12 freshly extracted human impacted third molars...
October 28, 2016: BMC Oral Health
https://www.readbyqxmd.com/read/27790081/regenerative-dentistry-animal-model-for-regenerative-endodontology
#8
Nisarat Ruangsawasdi, Matthias Zehnder, Raphael Patcas, Chafik Ghayor, Franz E Weber
BACKGROUND: Ectopic tissue has been observed frequently in human root canal specimens when cell homing studies were performed at the dorsum of rodents. In contrast, pulp-like tissue formed when immature teeth were implanted on top of the rat calvaria. It was surmised, yet not tested, that the implantation site might affect tissue ingrowth. METHODS: Four root sections from human immature molars cleaned with 5% sodium hypochlorite (NaOCl) followed by 17% ethylenediaminetetraacetic acid (EDTA) were implanted per rat (n = 5)...
September 2016: Transfusion Medicine and Hemotherapy
https://www.readbyqxmd.com/read/27787957/epiprofin-regulates-enamel-formation-and-tooth-morphogenesis-by-controlling-epithelial-mesenchymal-interactions-during-tooth-development
#9
Takashi Nakamura, Lucia Jimenez-Rojo, Eiki Koyama, Maurizio Pacifici, Susana de Vega, Masahiro Iwamoto, Satoshi Fukumoto, Fernando Unda, Yoshihiko Yamada
The synchronization of cell proliferation and cytodifferentiation between dental epithelial and mesenchymal cells is required for the morphogenesis of teeth with the correct functional shapes and optimum sizes. Epiprofin (Epfn), a transcription factor belonging to the Sp family, regulates dental epithelial cell proliferation and is essential for ameloblast and odontoblast differentiation. Epfn deficiency results in the lack of enamel and ironically the formation of extra teeth. We investigated the mechanism underlying the functions of Epfn in tooth development through the creation of transgenic mice expressing Epfn under the control of an epithelial cell-specific K5 promoter, (K5-Epfn)...
October 27, 2016: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/27784228/a-perspective-on-stem-cells-as-biological-systems-that-produce-differentiated-osteoblasts-and-odontoblasts
#10
Shahrul Hisham Zainal Ariffin, Thanaletchumi Manogaran, Intan Zarina Zainol Abidin, Rohaya Megat Abdul Wahab, Sahidan Senafi
Stem cells (SCs) are capable of self-renewal and multilineage differentiation. Human mesenchymal stem cells (MSCs) and haematopoietic stem cells (HSCs) which can be obtained from multiple sources, are suitable for application in regenerative medicine and transplant therapy. The aim of this review is to evaluate the potential of genomic and proteomic profiling analysis to identify the differentiation of MSCs and HSCs towards osteoblast and odontoblast lineages. In vitro differentiation towards both of these lineages can be induced using similar differentiation factors...
October 26, 2016: Current Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27776430/baicalein-promotes-angiogenesis-and-odontoblastic-differentiation-via-the-bmp-and-wnt-pathways-in-human-dental-pulp-cells
#11
Sang-Im Lee, Sun-Young Kim, Kyung-Ran Park, Eun-Cheol Kim
Baicalein is an active flavonoid extracted from the root of Scutellaria baicalensis that has anticancer and anti-inflammatory properties; its effects on osteoblastic and angiogenic potential are controversial. The aim of this study was to investigate the effects of baicalein on odontoblastic differentiation and angiogenesis and the underlying mechanism in human dental pulp cells (HDPCs). Baicalein (1-10[Formula: see text][Formula: see text]M) had no cytotoxic effects and promoted alkaline phosphatase (ALP) activity, mineralization assayed by Alizarin Red-S staining, and the mRNA expression of marker genes, in a concentration-dependent manner...
October 25, 2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27747243/functional-roles-of-nod1-in-odontoblasts-on-dental-pulp-innate-immunity
#12
Yuki Hosokawa, Kouji Hirao, Hiromichi Yumoto, Ayako Washio, Tadashi Nakanishi, Daisuke Takegawa, Chiaki Kitamura, Takashi Matsuo
Caries-related pathogens are first recognized by odontoblasts and induce inflammatory events that develop to pulpitis. Generally, initial sensing of microbial pathogens is mediated by pattern recognition receptors, such as Toll-like receptor and nucleotide-binding oligomerization domain (NOD); however, little is known about NODs in odontoblasts. In this study, the levels of NODs expressed in rat odontoblastic cell line, KN-3, were assessed by flow cytometry and the levels of chemokines in NOD-specific ligand-stimulated KN-3 cells were analyzed by real-time PCR and ELISA...
2016: BioMed Research International
https://www.readbyqxmd.com/read/27744048/pulp-and-apical-tissue-response-to-deep-caries-in-immature-teeth-a-histologic-and-histobacteriologic-study
#13
Domenico Ricucci, José F Siqueira, Simona Loghin, Louis M Lin
: Descriptions of the pathologic changes in the pulp and associated apical structures of human immature teeth in response to deep caries are lacking in the literature. OBJECTIVES: This article describes the histologic events associated with the radicular pulp and the apical tissues of human immature teeth following pulp inflammation and necrosis. METHODS: Twelve immature teeth with destructive caries lesions were obtained from 8 patients. Two intact immature teeth served as controls...
October 12, 2016: Journal of Dentistry
https://www.readbyqxmd.com/read/27734382/cell-biological-assays-for-measuring-odontogenic-activities-of-ccn-proteins
#14
Koichiro Muromachi, Hiroshi Sugiya, Nobuyuki Tani-Ishii
In postnatal dentin formation, odontoblast differentiation occurs in the pulp tissue regenerative process under pathological condition. Odontoblasts and newly differentiated odontoblast-like cells beneath the caries lesion form tertiary dentin and are highly odontogenic. To observe the activity of dentinogenesis occur within the hard tissue, a combination of immunohistological analysis and immunodetection of dentinogenesis specific molecules, such as dentin sialophosphoprotein (DSPP) and/or its cleaved products dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), is a reliable approach...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27722013/requirement-of-smad4-mediated-signaling-in-odontoblast-differentiation-and-dentin-matrix-formation
#15
Chi-Young Yun, Hwajung Choi, Young-Jae You, Jin-Young Yang, Jin-A Baek, Eui-Sic Cho
Dentin is the major part of tooth and formed by odontoblasts. Under the influence of the inner enamel epithelium, odontoblasts differentiate from ectomesenchymal cells of the dental papilla and secrete pre-dentin which then undergo mineralization into dentin. Transforming growth factor-beta (TGF-β)/bone morphogenetic protein (BMP) signaling is essential for dentinogenesis; however, the precise molecular mechanisms remain unclear. To understand the role of TGF-β/BMP signaling in odontoblast differentiation and dentin formation, we generated mice with conditional ablation of Smad4, a key intracellular mediator of TGF-β/BMP signaling, using Osr2 or OC-Cre mice...
September 2016: Anatomy & Cell Biology
https://www.readbyqxmd.com/read/27718420/near-infrared-laser-increases-mdpc-23-odontoblast-like-cells-proliferation-by-activating-redox-sensitive-pathways
#16
Manuela Rizzi, Mario Migliario, Vincenzo Rocchetti, Stelvio Tonello, Filippo Renò
Near infrared laser is known to induce biostimulatory effects, resulting in cell proliferation enhancement. Although such positive effect is widely exploited in various clinical applications, molecular mechanisms involved are still poorly understood. The aim of the study was to investigate the ability of laser stimulation to increase cell proliferation through an early activation of three redox sensitive pathways, namely Nrf-2, NF-κB and ERK in a rat odontoblast-like cell line (MDPC-23 cells). MDPC-23 cells were irradiated with different energy settings (0-50J, corresponding to 0-32...
September 30, 2016: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/27694154/extracellular-atp-induces-calcium-signaling-in-odontoblasts
#17
B M Lee, H Jo, G Park, Y H Kim, C K Park, S J Jung, G Chung, S B Oh
Odontoblasts form dentin at the outermost surface of tooth pulp. An increasing level of evidence in recent years, along with their locational advantage, implicates odontoblasts as a secondary role as sensory or immune cells. Extracellular adenosine triphosphate (ATP) is a well-characterized signaling molecule in the neuronal and immune systems, and its potential involvement in interodontoblast communications was recently demonstrated. In an effort to elaborate the ATP-mediated signaling pathway in odontoblasts, the current study performed single-cell reverse transcription polymerase chain reaction (RT-PCR) and immunofluorescent detection to investigate the expression of ATP receptors related to calcium signal in odontoblasts from incisal teeth of 8- to 10-wk-old rats, and demonstrated an in vitro response to ATP application via calcium imaging experiments...
September 30, 2016: Journal of Dental Research
https://www.readbyqxmd.com/read/27692439/cvd-grown-monolayer-graphene-induces-osteogenic-but-not-odontoblastic-differentiation-of-dental-pulp-stem-cells
#18
Han Xie, Melissa Chua, Intekhab Islam, Ricardo Bentini, Tong Cao, José Carlos Viana-Gomes, Antônio Hélio Castro Neto, Vinicius Rosa
OBJECTIVE: The objective was to investigate the potential of graphene (Gp) to induce odontogenic and osteogenic differentiation in dental pulp stem cells (DPSC). METHODS: Gp was produced by chemical vapor deposition. DPSC were seeded on Gp or glass (Gl). Cells were maintained in culture medium for 28 days. Every two days, culture medium from Gp was used to treat cells on Gl and vice versa. Mineralization and differentiation of DPSC on all substrates were evaluated after 14 and 28 days by alizarin red S staining, qPCR, immunofluorescence and FACS...
September 27, 2016: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/27651223/hyaluronan-induces-odontoblastic-differentiation-of-dental-pulp-stem-cells-via-cd44
#19
Naoki Umemura, Emika Ohkoshi, Masamichi Tajima, Hirotaka Kikuchi, Tadashi Katayama, Hiroshi Sakagami
BACKGROUND: Dental pulp tissue contains many undifferentiated mesenchymal cells, which retain the ability to differentiate into mature cells. Induced pluripotent stem cells have been developed from various cell sources, including dental pulp-derived stem cells, and evaluated for potential application to regenerative therapy. Dental pulp tissues overexpress CD44, a cell-adhesion factor involved in the induction of mineralization. In this study, we investigated the effects of hyaluronan-a known CD44 ligand-on dental pulp stem cells (DPSCs)...
September 20, 2016: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/27639333/gelatin-scaffold-combined-with-bone-morphogenetic-protein-4-induces-odontoblast-like-cell-differentiation-involving-integrin-profile-changes-autophagy-related-gene-10-and-wnt5-sequentially-in-human-induced-pluripotent-stem-cells
#20
Nobuaki Ozeki, Naoko Hase, Naoya Higuchi, Taiki Hiyama, Hideyuki Yamaguchi, Rie Kawai, Toru Matsumoto, Kazuhiko Nakata, Makio Mogi
While human induced pluripotent stem (hiPS) cells have potential use in regenerative medicine, there are no reports on odontoblastic differentiation of hiPS cells. In the current study, to examine integrin profiles and explore the early signaling cascade of odontoblastic differentiation in hiPS cells, we investigated the regulation of autophagy-related gene (Atg) and wingless/int1 (Wnt) signaling in gelatin scaffold (GS) combined with bone morphogenetic protein (BMP)-4 (GS/BMP-4)-mediated odontoblastic differentiation...
September 15, 2016: Differentiation; Research in Biological Diversity
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