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https://www.readbyqxmd.com/read/28538680/diazonium-salt-based-surface-enhanced-raman-spectroscopy-nanosensor-detection-and-quantitation-of-aromatic-hydrocarbons-in-water-samples
#1
Inga Tijunelyte, Stéphanie Betelu, Jonathan Moreau, Ioannis Ignatiadis, Catherine Berho, Nathalie Lidgi-Guigui, Erwann Guénin, Catalina David, Sébastien Vergnole, Emmanuel Rinnert, Marc Lamy de la Chapelle
Here, we present a surface-enhanced Raman spectroscopy (SERS) nanosensor for environmental pollutants detection. This study was conducted on three polycyclic aromatic hydrocarbons (PAHs): benzo[a]pyrene (BaP), fluoranthene (FL), and naphthalene (NAP). SERS substrates were chemically functionalized using 4-dodecyl benzenediazonium-tetrafluoroborate and SERS analyses were conducted to detect the pollutants alone and in mixtures. Compounds were first measured in water-methanol (9:1 volume ratio) samples. Investigation on solutions containing concentrations ranging from 10(-6) g L(-1) to 10(-3) g L(-1) provided data to plot calibration curves and to determine the performance of the sensor...
May 24, 2017: Sensors
https://www.readbyqxmd.com/read/28534282/template-free-synthesis-of-phosphate-based-spheres-via-modified-supersaturated-phosphate-buffer-solutions
#2
Tao Qin, Yuanyuan Han, Peng Zhang, Ishtiaq Hassan Wani, Fredrik Nikolajeff, Klaus Leifer, Håkan Engqvist
Modified supersaturated phosphate buffer solutions were used to synthesize phosphate-based spheres, including calcium phosphate (CaP), strontium phosphate (SrP) and barium phosphate (BaP). A series of ions concentrations in the modified phosphate buffer solutions were investigated in order to study their effects in precipitates morphologies. During synthesis, it was found that magnesium was the key factor in sphere formation. The morphologies of calcium phosphate, strontium phosphate and barium phosphate precipitates varied as the concentration of magnesium ions varied...
July 2017: Journal of Materials Science. Materials in Medicine
https://www.readbyqxmd.com/read/28528714/the-use-of-stomas-in-the-early-management-of-hirschsprung-s-disease-findings-of-a-national-prospective-cohort-study
#3
T J Bradnock, M Knight, S Kenny, M Nair, G M Walker
BACKGROUND/PURPOSE: Primary pull-through without a stoma has become preferred practice in managing Hirschsprung's disease (HD). The aims of this study were to establish stoma rate and identify factors associated with stoma formation in a population-based cohort in the UK and Ireland. METHODS: Live-born infants with HD were prospectively identified in all 28 specialist pediatric surgical units in the UK and Ireland between October 2010 to September 2012. Method of colonic decompression was recorded and multivariable logistic regression was used to identify factors associated with stoma formation...
May 11, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28508284/repeatability-of-quantitative-parameters-of-18f-fluoride-pet-ct-and-biochemical-tumour-and-specific-bone-remodelling-markers-in-prostate-cancer-bone-metastases
#4
Cecilia Wassberg, Mark Lubberink, Jens Sörensen, Silvia Johansson
PURPOSE: 18F-fluoride PET/CT exhibits high sensitivity to delineate and measure the extent of bone metastatic disease in patients with prostate cancer. 18F-fluoride PET/CT could potentially replace traditional bone scintigraphy in clinical routine and trials. However, more studies are needed to assess repeatability and biological uptake variation. The aim of this study was to perform test-retest analysis of quantitative PET-derived parameters and blood/serum bone turnover markers at the same time point...
December 2017: EJNMMI Research
https://www.readbyqxmd.com/read/28503322/changes-in-oxidized-ldl-during-a-half-marathon-in-athletes-with-spinal-cord-injuries
#5
Toshihito Mitsui, Tomoyuki Ito, Yusuke Sasaki, Takashi Kawasaki, Takeshi Nakamura, Yukihide Nishimura, Tatsuru Ibusuki, Yukiharu Higuchi, Sayoko Hosoe, Fumiaki Ito, Fumihiro Tajima
INTRODUCTION: We reported previously that exercise significantly increases plasma adrenaline and oxidized low-density lipoprotein (oxLDL) in healthy subjects but not in persons with spinal cord injury (SCI). Since oxLDL and adrenaline levels are associated with oxidant/antioxidant balance, and exercise training elicits production of reactive oxygen species, we elucidated the effects of exercise on adrenaline, oxidant/antioxidant balance and oxLDL in individuals with SCI. CASE PRESENTATION: Eight subjects with cervical spinal cord injury (CSCI) and nine subjects with lower lesion of SCI (lower SCI (LSCI)) participated in a wheelchair half marathon race, and blood samples were collected before (pre), immediately after (post) and 1 h after the race (post 1 h)...
2017: Spinal Cord Series and Cases
https://www.readbyqxmd.com/read/28502747/chicken-egg-fetal-liver-dna-and-histopathologic-effects-of-structurally-diverse-carcinogens-and-non-carcinogens
#6
M J Iatropoulos, T Kobets, J-D Duan, K D Brunnemann, E Vock, U Deschl, G M Williams
Chicken egg fetal livers were evaluated for histopathological changes produced by four genotoxic hepatocarcinogens: 2-acetylaminofluorene (AAF), aflatoxin B1 (AFB1), benzo[a]pyrene (BaP), diethylnitrosamine (DEN); four structurally related non- or weakly- carcinogenic comparators: fluorene (FLU), aflatoxin B2 (AFB2), benzo[e]pyrene (BeP), N-nitrosodiethanolamine (NDELA); two epigenetic hepatocarcinogens: clofibric acid (CFA), phenobarbital (PB); and the non-carcinogen, D-mannitol (MAN). CFA, PB and MAN were also assessed for formation of DNA adducts using the (32)P nucleotide postlabeling (NPL) assay and for DNA breaks using the comet assay...
May 11, 2017: Experimental and Toxicologic Pathology: Official Journal of the Gesellschaft Für Toxikologische Pathologie
https://www.readbyqxmd.com/read/28502037/the-role-of-the-broader-autism-phenotype-and-environmental-stressors-in-the-adjustment-of-siblings-of-children-with-autism-spectrum-disorders-in-taiwan-and-the-united-kingdom
#7
Hsiao-Wei Joy Tsai, Katie Cebula, Sue Fletcher-Watson
The influence of the broader autism phenotype (BAP) on the adjustment of siblings of children with autism has previously been researched mainly in Western cultures. The present research evaluated a diathesis-stress model of sibling adjustment using a questionnaire study including 80 and 75 mother-typically developing sibling dyads in Taiwan and the United Kingdom (UK). UK siblings reported elevated adjustment difficulties compared to the Taiwanese sample and to normative data. Whilst higher BAP levels were generally associated with greater adjustment difficulties, differences were found across cultures and respondents...
May 13, 2017: Journal of Autism and Developmental Disorders
https://www.readbyqxmd.com/read/28498123/comparison-of-phosphorus-fraction-distribution-and-influencing-factors-of-suspended-and-surface-sediments-in-the-tiaoxi-watershed-china
#8
Hongmeng Ye, Xuyin Yuan, Lei Han, Heng Yin, Jing Jin
Suspended and surface sediments from the Tiaoxi watershed, fed by the Xitiaoxi and Dongtiaoxi rivers, were analyzed for total phosphorus (TP) and the inorganic P fractions of loosely adsorbed P that were extractable with NH4Cl (NH4Cl-P), reductant P (BD-P), metal oxide-bound P extractable with NaOH (NaOH-P), and calcium-bound, HCl-extractable P (HCl-P), while other physicochemical compositions were also determined. The spatial variations of P fractions in these sediments were investigated, and the major factors influencing the various fractions were explored by multivariate statistics...
May 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28494841/quercetin-attenuates-benzo-%C3%AE-pyrene-induced-cyp1a-expression
#9
M L Perepechaeva, T A Seredina, Y A Sidorova, E N Pivovarova, A L Markel, V V Lyakhovich, A Y Grishanova
We studied effects of nutrient quercetin on cytochromes' Р450 1А (CYP1A) activities (measured spectrofluorimetrically using 7-ethoxy-resorufin for CYP1A1 and 7-methoxy-resorufin for CYP1A2 as substrates), on mRNA levels (measured by RT-PCR), and on DNA-binding activities (evaluated by an electrophoretic mobility shift assay) of proteins regulating CYP1A expression in untreated and benzo(α)pyrene (BaP)-treated rats. Wistar rats received quercetin, BaP, or both once daily for 1-3 days. Quercetin did not influence CYP1A1 in untreated rats but inhibited BaP-mediated CYP1A induction on the transcriptional level decreasing positive input (AhR functional activity) and increasing negative input (AhRR/ARNT expression and Oct-1 and C/EBP functional activities)...
April 2017: Biomedical and Environmental Sciences: BES
https://www.readbyqxmd.com/read/28486552/identification-of-novel-mirnas-and-mirna-expression-profiling-in-embryogenic-tissues-of-picea-balfouriana-treated-by-6-benzylaminopurine
#10
Qingfen Li, Cheng Deng, Yan Xia, Lisheng Kong, Hanguo Zhang, Shougong Zhang, Junhui Wang
Here, we compared miRNA expression profiles in embryonic cell cultures of the conifer Picea balfouriana following application of the synthetic cytokinin 6-benzylaminopurine (6-BAP). We used next-generation sequencing to analyze three libraries of small RNAs from the treated embryogenic cell cultures and generated 24,000,000 raw reads from each of the libraries. Over 70 differentially regulated micro RNA (miRNA) families (≥2 fold change in expression) were identified between pairs of treatments. A quantitative analysis showed that miR3633 and miR1026 were upregulated in tissues with the highest embryogenic ability...
2017: PloS One
https://www.readbyqxmd.com/read/28486336/seasonal-variations-of-bone-metabolism-and-bone-mineral-density-in-collegiate-alpine-skiers
#11
Teruyuki Sato, Keishoku Sakuraba, Yosuke Tsuchiya, Shinya Maruyama, Eisuke Ochi
This study investigated the seasonal variations in bone metabolism, mineral density, and physical characteristics in collegiate alpine skiers. Twelve male subjects (20.6 ± 1.5 years) were evaluated for body composition, body mineral density (BMD), serum levels of osteocalcin (OC), bone alkaline phosphatase (BAP), tartrate-resistant acid phosphatase 5b (TRACP-5b), parathormone, and 25-hydroxyvitamin D (25(OH)D). We also measured the knee extensor and flexor strength, maximal power, and vertical jump. Measurements were conducted in the pre-season period (T1), competitive period (T2), end-of-season period (T3), and resting period (T4)...
May 5, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28482256/characterization-of-pahs-in-size-fractionated-submicron-atmospheric-particles-and-their-association-with-the-intracellular-oxidative-stress
#12
Qinghua Zhou, Baichen Liu, Yimei Chen, Xiaoqian Han, Xiuzhen Wei, Ying Zhu, Xing Zhou, Jinyuan Chen
The submicron atmospheric particulate matters (PMs), consisting of 7 size-resolved fractions, were collected by two Dekati low pressure impactors from December 2015 to January 2016 in Hangzhou, China. The chemical analysis revealed the higher accumulation efficiency of polycyclic aromatic hydrocarbons (PAHs) in the finer submicron PMs, and 77.0% of particulate PAHs in PM1 were associated with PM0.4. Moreover, the BaP equivalent concentrations (BaPeq) were evaluated for size-fractionated submicron PMs, indicating that 77...
April 29, 2017: Chemosphere
https://www.readbyqxmd.com/read/28479728/in-vitro-toxicity-and-genotoxic-activity-of-aqueous-leaf-extracts-from-four-varieties-of-olea-europea-l
#13
Luc Verschaeve, Hayet Edziri, Roel Anthonissen, Dalenda Boujnah, Fethia Skhiri, Hechmi Chehab, Mahjoub Aouni, Maha Mastouri
AIM: Despite its therapeutic value almost nothing is known about potential adverse health effects of Olea europea L. We therefore investigated the in vitro toxicity and genotoxicity of leaf extracts of this plant. MATERIAL AND METHODS: Extracts from olive tree leaves were obtained from four different regions in Tunisia. We investigated the in vitro toxicity, genotoxicity and antigenotoxicity of their aqueous extracts using the neutral red (NR) uptake, Vitotox and alkaline comet assays...
January 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28479241/ototoxicity-of-boric-acid-powder-in-a-rat-animal-model
#14
Murat Salihoglu, Salim Dogru, Enver Cesmeci, Halil Caliskan, Onuralp Kurt, Zafer Kuçukodaci, Atila Gungor
INTRODUCTION: Boric acid, which has antiseptic and acidic properties, is used to treat external and middle ear infections. However, we have not found any literature about the effect of boric acid powder on middle ear mucosa and inner ear. OBJECTIVE: The purpose of this study is to investigate possible ototoxic effects of boric acid powder (BAP) on cochlear outer hair cell function and histological changes in middle ear mucosa in a rat animal model. METHODS: Twenty healthy, mature Wistar albino rats were used in this study...
April 22, 2017: Brazilian Journal of Otorhinolaryngology
https://www.readbyqxmd.com/read/28476648/the-polycyclic-aromatic-hydrocarbons-benzo-a-pyrene-and-phenanthrene-inhibit-intestinal-lipase-activity-in-rainbow-trout-oncorhynchus-mykiss
#15
Stefan de Gelder, Øystein Sæle, Bas T H de Veen, Joëlle Vos, Gert Flik, Marc H G Berntssen, Peter H M Klaren
Elevated levels of polycyclic aromatic hydrocarbons (PAHs) are detected in aquafeeds where fish oils are (partially) replaced by vegetable oils. The highly lipophilic PAHs solubilize readily in oil droplets and micelles in the intestinal lumen that can affect enzymatic lipid digestion by altering lipase activity. We therefore investigated the effect of two PAHs, benzo[a]pyrene (BaP) and phenanthrene (PHE), on bile salt-activated lipase (BAL) activity in desalted luminal extracts of the proximal intestine of rainbow trout (Oncorhynchus mykiss) using the triacylglycerides rapeseed oil and fish oil as substrates...
May 3, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28476633/dna-methylation-in-lung-tissues-of-mouse-offspring-exposed-in-utero-to-polycyclic-aromatic-hydrocarbons
#16
Trevor J Fish, Abby D Benninghoff
Polycyclic aromatic hydrocarbons (PAHs) comprise an important class of environmental pollutants that are known to cause lung cancer in animals and are suspected lung carcinogens in humans. Moreover, evidence from cell-based studies points to PAHs as modulators of the epigenome. The objective of this work was to assess patterns of genome-wide DNA methylation in lung tissues of adult offspring initiated in utero with the transplacental PAH carcinogens dibenzo [def,p]chrysene (DBC) or benzo [a]pyrene (BaP). Genome-wide methylation patterns for normal (not exposed), normal adjacent and lung tumor tissues obtained from adult offspring were determined using methylated DNA immunoprecipitation (MeDIP) with the NimbleGen mouse DNA methylation CpG island array...
May 2, 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28473963/rank-ligand-and-osteoprotegerin-as-biomarkers-in-the-differentiation-between-periprosthetic-joint-infection-and-aseptic-prosthesis-loosening
#17
Max J Friedrich, Matthias D Wimmer, Jan Schmolders, Andreas C Strauss, Milena M Ploeger, Hendrik Kohlhof, Dieter C Wirtz, Sascha Gravius, Thomas M Randau
AIM: To assess serum levels of RANK-ligand (RANKL) and osteoprotegerin (OPG) as biomarkers for periprosthetic joint infection (PJI) and compare their accuracy with standard tests. METHODS: One hundred and twenty patients presenting with a painful total knee or hip arthroplasty with indication for surgical revision were included in this prospective clinical trial. Based on standard diagnostics (joint aspirate, microbiological, and histological samples) and Musculoskeletal Infection Society consensus classification, patients were categorized into PJI, aseptic loosening, and control groups...
April 18, 2017: World Journal of Orthopedics
https://www.readbyqxmd.com/read/28468005/noninvasive-blood-pressure-measurement-in-maintenance-hemodialysis-patients-comparison-of-agreement-between-oscillometric-and-finger-cuff-methods
#18
Gaetano Alfano, Francesco Fontana, Gianni Cappelli
BACKGROUND: The Nexfin monitor is a device used for measuring arterial blood pressure (BP) continuously and noninvasively through finger-cuff technology. Since it was validated against the auscultatory method using a sphygmomanometer, the aim of this study was to test the ability of the Nexfin to evaluate brachial arterial pressure (BAP) in maintenance hemodialysis patients, quantifying accuracy and precision. METHODS: Forty hemodynamically stable hemodialysis patients underwent serial measurements of Nexfix arterial pressure (NAP) and BAP, respectively, through Nexfin and oscillometric devices...
May 4, 2017: Nephron
https://www.readbyqxmd.com/read/28467299/serum-level-of-antibodies-igg-igm-against-benzo-a-pyrene-7-8-diol-9-10-epoxide-dna-adducts-in-children-dermatologically-exposed-to-coal-tar
#19
Pavel Borský, Ctirad Andrýs, Jan Krejsek, Květoslava Hamáková, Jan Kremláček, Andrea Málková, Lenka Bartošová, Zdeněk Fiala, Vladimír Palička, Lenka Borská
Crude coal tar (CCT) contains polycyclic aromatic hydrocarbons (PAHs). Benzo[a]pyrene (BaP) is metabolized into a highly reactive metabolite benzo[a]pyrene-7,8-diol-9,10-epoxide (BPDE) that is able to bind to DNA and creates BPDE-DNA adducts. Adducted DNA becomes immunogenic and induces immune response by production of antibodies against BPDE-DNA adducts (Ab-BPDE-DNA). Circulating Ab-BPDE-DNA was proposed as potential biomarker of genotoxic exposure to BaP (PAHs). Goeckerman therapy (GT) of psoriasis uses dermal application of CCT ointment (PAHs)...
May 3, 2017: Acta Medica (Hradec Králové)
https://www.readbyqxmd.com/read/28467152/the-interaction-between-glucose-and-cytokinin-signaling-in-controlling-arabidopsis-thaliana-seedling-root-growth-and-development
#20
Sunita Kushwah, Ashverya Laxmi
Cytokinin (CK) and glucose (GLC) control several common responses in plants. There is an extensive overlap between CK and GLC signal transduction pathways in Arabidopsis. Physiologically, both GLC and CK could regulate root length in light. CK interacts with GLC via HXK1 dependent pathway for root length control. Wild-type (WT) roots cannot elongate in the GLC free medium while CK-receptor mutant ARABIDOPSIS HISTIDINE KINASE4 (ahk4) and type B ARR triple mutant ARABIDOPSIS RESPONSE REGULATOR1, 10,11 (arr1, 10,11) roots could elongate even in the absence of GLC as compared with the WT...
May 3, 2017: Plant Signaling & Behavior
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