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Qinqin Song, Hailong Zhou, Qian Han, Xiaoping Diao
Dichlorodiphenyltrichloroethane (DDT) and benzo(a)pyrene (BaP) are environmental estrogens (EEs) that are ubiquitous in the marine environment. In the present study, we integrated isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomic and nuclear magnetic resonance (NMR)-based metabolomic approaches to explore the toxic responses of green mussel hepatopancreas exposed to DDT (10μg/L), BaP (10μg/L) and their mixture. The metabolic responses indicated that BaP primarily disturbed energy metabolism and osmotic regulation in the hepatopancreas of the male green mussel P...
September 11, 2017: Aquatic Toxicology
Jong Kyu Woo, Hyun Jin Jung, Ji-Youn Park, Ju-Hee Kang, Byung Il Lee, DongYun Shin, Chu Won Nho, Soo-Young Cho, Je Kyung Seong, Seung Hyun Oh
FAK overexpression has been reported in diverse primary and metastatic tumor tissues, supporting its pro-tumorigenic and pro-metastatic roles. Therefore, we have developed a neo-treatment strategy using daurinol to effectively treat cancer metastasis. Daurinol blocked cancer cell migration and invasion in vitro and exhibited anti-metastatic activity in an experimental metastasis model of breast and lung cancer. Daurinol selectively inhibited phosphorylation of FAK at Tyr925, Tyr576/577, and Tyr397 sites in a dose- and time-dependent manner...
August 22, 2017: Oncotarget
Wei Qin, FuQiang Fan, Yi Zhu, Yingying Wang, Xiang Liu, Aizhong Ding, Junfeng Dou
High-molecular-weight polycyclic aromatic hydrocarbons are persistent organic pollutants with great environmental and human health risks and the associated bioremediation activities have always been hampered by the lack of powerful bacterial species under redox conditions. A Microbacterium sp. strain capable of using benzo(a)pyrene as sole carbon and energy sources under denitrifying conditions was isolated. The difference in protein expression during BaP removal and removal characterization were investigated...
September 14, 2017: Bioprocess and Biosystems Engineering
Jason Y Y Wong, Wei Hu, George S Downward, Wei Jie Seow, Bryan A Bassig, Bu-Tian Ji, Fusheng Wei, Guoping Wu, Jihua Li, Jun He, Chin-San Liu, Wen-Ling Cheng, Yunchao Huang, Kaiyun Yang, Ying Chen, Nathaniel Rothman, Roel C Vermeulen, Qing Lan
Households in Xuanwei and Fuyuan, China, possess hazardous levels of fine particulate matter with an aerodynamic diameter <2.5 microns (PM2.5) and polycyclic aromatic hydrocarbons (PAHs) from coal combustion. Previous studies found that increased exposure to PM2.5 and benzo[a]pyrene (BaP; a PAH) were associated with decreased mitochondrial DNA copy number (mtDNAcn), a marker of oxidative stress. We further evaluated these associations in a cross-sectional study of 148 healthy non-smoking women from Xuanwei and Fuyuan...
September 1, 2017: Carcinogenesis
Mianqun Zhang, Yilong Miao, Qian Chen, Meng Cai, Wenkang Dong, Xiaoxin Dai, Yajuan Lu, Changyin Zhou, Zhaokang Cui, Bo Xiong
Benzo[a]pyrene (BaP) is a ubiquitous environmental pollutant and carcinogen that is frequently found in particulate matter, with a diameter of ≤2.5 μm (PM2.5). It has been reported to interrupt the normal reproductive system, but the exact molecular basis has not been clearly defined. To understand the underlying mechanisms regarding how BaP exposure disrupts female fertility, we evaluated oocyte quality by assessing the critical regulators and events during oocyte meiotic maturation and fertilization. We found that BaP exposure compromised the mouse oocyte meiotic progression by disrupting normal spindle assembly, chromosome alignment, and kinetochore-microtubule attachment, consequently leading to the generation of aneuploid eggs...
September 13, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Jia Xie, Dinglong Yang, Xiyan Sun, Ruiwen Cao, Lizhu Chen, Qing Wang, Fei Li, Huifeng Wu, Chenglong Ji, Ming Cong, Jianmin Zhao
Persistent organic pollutants (POPs) are ubiquitous and coexisted in the aquatic environment. Individual and combined toxic effects of benzo[a]pyrene (BaP) and 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) on embryogenesis, and larval survival of the Pacific oyster were investigated. The EC50 values of BaP, BDE-47 and their mixture on embryogenesis were 18.4, 203.3 and 72.0 µg/L respectively, while the LC50 values for 96 h larval mortality were 26.8, 244.5 and 108.9 µg/L respectively. The Marking-Dawson additive toxicity indices were -0...
September 9, 2017: Bulletin of Environmental Contamination and Toxicology
Sunita J Varjani, Edgard Gnansounou, Ashok Pandey
Control and prevention of environmental pollution has become a worldwide issue of concern. Aromatic hydrocarbons including benzene, toluene, ethyl benzene, xylene (BTEX) and polyaromatic hydrocarbons (PAHs) are persistent organic pollutants (POPs), released into the environment mainly by exploration activities of petroleum industry. These pollutants are mutagenic, carcinogenic, immunotoxic and teratogenic to lower and higher forms of life i.e. microorganisms to humans. According to the International Agency for Research on Cancer (IARC) and United States Environmental Protection Agency (U...
September 4, 2017: Chemosphere
Evelyn Smit, Terezinha Souza, Danyel G J Jennen, Jos C S Kleinjans, Twan van den Beucken
DNA damage mediates widespread changes in transcription through activation or repression of transcription factors (TFs). However, the consequences of regulating specific TFs for the outcome of the DNA repair process remain incompletely understood. Here, we combined transcriptomics and TF binding prediction with functional genomics to identify TFs essential for adequate DNA repair in HepG2 liver cells after a non-cytotoxic dose of carcinogens benzo(a)pyrene (BaP) (2μM) and aflatoxin B1 (AFB1) (5μM). BaP and AFB1 induced a largely common transcriptional response, mediated by similar TFs...
September 4, 2017: Toxicology
Sumitra Kumari Choudhary, Ashok Kumar Patel, Harish, Smita Shekhawat, Narpat S Shekhawat
Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA)...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
Ammar Nebigh, Mohamed Elfethi Abed, Rihab Borji, Sonia Sahli, Slaheddine Sellami, Zouhair Tabka, Haithem Rebai
The aim of this study was to examine the relationship between bone mass and bone turnover markers with lean mass in pubescent soccer players. Two groups participated in this study 65 elite young soccer players that trained from 6 to 8 hours per week and 60 controls. Bone mineral density (BMD), bone mineral content (BMC) in the whole body, lower limbs, lumbar spine, and femoral neck, biochemical markers of osteocalcin (OC), bone-specific alkaline phosphatase (BAP), C-telopeptide type I collagen (CTX) and total lean mass were assessed...
September 5, 2017: Pediatric Exercise Science
M S Pádua, R S Santos, C R G Labory, V C Stein, E G Mendonça, E Alves, L V Paiva
Large-scale propagation of oil palm (Elaeis guineensis, Jacq.) is difficult due to its single apical meristem. Thus, obtaining plants is mainly through seed germination, and a long growing period is required before oil production is possible. An alternative to large-scale seedling production is indirect somatic embryogenesis. The aim of this study was to analyze the somatic embryogenesis process in oil palm (E. guineensis Jacq.) with amino acids and low concentrations of auxins. The Tenera hybrid was analyzed by cytochemical and ultrastructural methods and was used to regenerate oil palm plants...
September 4, 2017: Protoplasma
S Gauthami, Deepak Kumar, K S R SivaSai, Nagendra R Hegde
Biotinylation has been extensively used for antibody tagging, affinity-based purification, and in protein/DNA-protein interaction studies. Here we describe the use of biotinylation to study the turn-over of proteins in cells. We use the prokaryotic biotin ligase (BirA) to biotinylate the human leukocyte antigen (HLA)-A2 (A2) heavy chain (HC), which was engineered to contain a biotin acceptor peptide (BAP). Controlled availability of biotin in combination with visualization using streptavidin-conjugated peroxidase made it possible to detect biotinylated BAP-A2...
August 30, 2017: Immunology Letters
Rathanin Seng, Thawatchai Kitti, Rapee Thummeepak, Phattaraporn Kongthai, Udomluk Leungtongkam, Surat Wannalerdsakun, Sutthirat Sitthisak
Methicillin-resistant coagulase negative staphylococci (MR-CoNS) are the major cause of infectious diseases because of their potential ability to form biofilm and colonize the community or hospital environments. This study was designed to investigate the biofilm producing ability, and the presence of mecA, icaAD, bap and fnbA genes in MR-CoNS isolates. The MR-CoNS used in this study were isolated from various samples of community environment and five wards of hospital environments, using mannitol salt agar (MSA) supplemented with 4 μg/ml of oxacillin...
2017: PloS One
Caroline Dejean de la Bâtie, Valérie Barbier, Célina Roda, Anaïs Brassier, Jean-Baptiste Arnoux, Vassili Valayannopoulos, Anne-Sophie Guemann, Clément Pontoizeau, Stéphanie Gobin, Florence Habarou, Florence Lacaille, Jean-Paul Bonnefont, Pierre Canouï, Chris Ottolenghi, Pascale De Lonlay, Lisa Ouss
Propionic acidemia is the result of a deficiency in propionyl-CoA carboxylase activity. Chronic neurologic and cognitive complications frequently occur, but the psychiatric evolution of the disorder is not well documented. We conducted a pedopsychiatric evaluation of 19 children, adolescents and young adults, aged between 2 and 25 years, using ADI-R, CARS-T, as well as ADOS when autism spectrum disorder was suspected. Previous psychometric examinations were also taken into consideration. Thirteen patients had an IQ < 80...
August 30, 2017: Journal of Inherited Metabolic Disease
Mehrzad Keshavarzifard, Farid Moore, Behnam Keshavarzi, Reza Sharifi
Surface sediment samples were collected from intertidal zone of Asaluyeh, Persian Gulf, to investigate distribution, sources and health risk of sixteen polycyclic aromatic hydrocarbons (PAHs). Total PAH concentrations ranged from 1.8 to 81.2 μg kg(-1) dry weight, which can be categorized as low level of pollution. Qualitative and quantitative assessments showed that PAHs originated from both petrogenic and pyrogenic sources with slight pyrogenic dominance. Source apportionment using principal component analysis indicated that the main sources of PAHs were fossil fuel combustion (33...
August 30, 2017: Environmental Geochemistry and Health
Xiaoping Wang, Zheng Zong, Chongguo Tian, Yingjun Chen, Chunling Luo, Jianhui Tang, Jun Li, Gan Zhang
A total of 76PM2.5 samples collected at Tuoji Island from November 2011 to January 2013 were used to analyze 15 congeners of polycyclic aromatic hydrocarbons (∑15PAHs) and assess their toxic potency. The average ∑15PAHs was 15.34±8.87ngm(-3), ranging from 4.24 to 40.62ngm(-3) over the sampling period. BkF, BbF, Phe and BaP were dominant PAH congeners, contributing together 60.64% of the ∑15PAH concentration. The highest monthly ∑15PAHs concentration was in January 2012, followed by the next January, which was closely four times greater than the lowest level occurred in July 2012...
August 27, 2017: Science of the Total Environment
Feng Jin, Jose Thaiparambil, Sri Ramya Donepudi, Venkat Rao Vantaku, Danthasinghe Waduge Badrajee Piyarathna, Suman Maity, Rashmi Krishnapuram, Vasanta Putluri, Franklin Gu, Preeti Purwaha, Salil Kumar Bhowmik, Chandra Shekar Reddy Ambati, Friedrich-Carl von Rundstedt, Florian Roghmann, Sebastian Berg, Joachim Noldus, Kimal Rajapakshe, Daniel Gödde, Stephan Roth, Stephan Störkel, Stephan Degener, George Michailidis, Benny A Kaipparettu, Balasubramanyam Karanam, Martha K Terris, Shyam M Kavuri, Seth P Lerner, Farrah Kheradmand, Cristian Coarfa, Arun Sreekumar, Yair Lotan, Randa El-Zein, Nagireddy Putluri
Smoking is a major risk factor for the development of Bladder Cancer (BLCA); however, the functional consequences of the carcinogens in tobacco smoke and BLCA-associated metabolic alterations remains poorly defined. We assessed the metabolic profiles in BLCA smokers and non-smokers, and identified the key alterations in their metabolism. Liquid Chromatography - Mass Spectrometry (LC-MS), and bioinformatic analysis were performed to determine the metabolome associated with BLCA smokers and were further validated in cell line models...
August 29, 2017: Cancer Prevention Research
Lewis Dunnigan, Benjamin J Morton, Philip J van Eyk, Peter J Ashman, Xiangping Zhang, Philip Anthony Hall, Chi Wai Kwong
The aim of this study was to evaluate the emissions of polycyclic aromatic hydrocarbons (PAHs) bound to the particulate matter (PM) during the combustion of raw pyrolysis volatiles (bio-oil and pyrogas mixture) generated from the pyrolysis of rice husk. Five different raw pyrolysis volatiles were produced at varying pyrolysis temperatures (400-800°C) and subsequently combusted in a laboratory-scale, continuous pyrolysis-combustion facility at 850°C. 15 priority pollutant PAH levels in the resulting biochar, bio-oil, and PM were evaluated...
November 2017: Bioresource Technology
Zhou Zhou, Jie Yang, King Ming Chan
Triclosan (TCS, 5-chloro-2-(2,4-dichlorophenoxy) phenol) is an antimicrobial agent widely used in personal care products. It has been detected in surface water, soil, aquatic species, and even humans. In this study, we used zebrafish (Danio rerio) as a model to test the hypothesis that TCS exhibits toxic effects by interacting with thyroid hormone receptor β (TRβ) and aryl hydrocarbon receptor (AhR) and by inducing the transcription of thyroid hormone (TH)-associated genes and affecting phase I and phase II enzymes...
August 19, 2017: Aquatic Toxicology
Agnieszka Baran, Marek Tarnawski, Krzysztof Urbański, Agnieszka Klimkowicz-Pawlas, Iwona Spałek
The aims of the study were to investigate the concentration, sources and ecological risk of PAHs (polycyclic aromatic hydrocarbons) in bottom sediments collected from nine reservoirs located in south-eastern Poland. The concentration of ∑PAHs in sediments ranged from 150 to 33,900 μg kg(-1). The total PAH concentration in the bottom sediments was arranged in the following order: Rybnik > Rzeszów > Brzóza Królewska > Brzóza Stadnicka > Besko > Chechło > Ożanna > Głuchów > Narożniki...
August 21, 2017: Environmental Science and Pollution Research International
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