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https://www.readbyqxmd.com/read/28324674/osteoblast-osteoclast-interactions
#1
Xiao Chen, Zhongqiu Wang, Na Duan, Guoying Zhu, Edward M Schwarz, Chao Xie
Bone homeostasis depends on the resorption of bones by osteoclasts and formation of bones by the osteoblasts. Imbalance of this tightly coupled process can cause diseases such as osteoporosis. Thus, the mechanisms that regulate communication between osteoclasts and osteoblasts are critical to bone cell biology. It has been shown that osteoblasts and osteoclasts can communicate with each other through direct cell-cell contact, cytokines, and extracellular matrix interaction. Osteoblasts can affect osteoclast formation, differentiation, or apoptosis through several pathways, such as OPG/RANKL/RANK, RANKL/LGR4/RANK, Ephrin2/ephB4, and Fas/FasL pathways...
February 8, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28320782/diabetes-confers-in-vitro-calcific-potential-on-serum-which-associates-with-in-vivo-vascular-calcification
#2
Ashish Patidar, Dhruv K Singh, Shori Thakur, Peter Winocour, Ken Farrington, Anwar Baydoun
Although vascular calcification (VC) is prevalent in type 2 diabetes (T2DM), underlying mechanisms remain unclear. Neither is it known whether T2DM confers calcific potential (CP) to serum, enabling it to induce VC outside the disease milieu. We therefore investigated the CP of serum from controls and subjects with T2DM with and without in vivo VC . Samples from 20 healthy controls and 45 age- and sex-matched patients with T2DM with MDRD-eGFR>60mL min(-1) were analysed for CP using rat aortic smooth muscle cells in vitro CT scans of femoral arteries identified individuals with in vivo calcification...
March 20, 2017: Clinical Science (1979-)
https://www.readbyqxmd.com/read/28318924/aged-rats-under-zoledronic-acid-therapy-and-oral-surgery
#3
Mariza Akemi Matsumoto, Elisa Mara de Abreu Furquim, Alaíde Gonçalves, Joel Ferreira Santiago-Júnior, Patrícia Pinto Saraiva, Camila Lopes Cardoso, Marcelo Salles Munerato, Roberta Okamoto
INTRODUCTION: Aging brings a number of health conditions that can compromise the healing process in elderly individuals, significantly when it comes to bone tissue. The aim of the present study was to analyze the effects of zoledronic acid (ZL) therapy on socket healing of aged male rats. MATERIALS AND METHODS: Twenty-four Wistar male rats, 20 months old, underwent surgical procedures for the extraction of the upper right incisor and were divided into two groups according to the treatment: Control (C) - intravenous (IV) 0...
February 17, 2017: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/28317941/magnitude-dependent-response-of-osteoblasts-regulated-by-compressive-stress
#4
Xiao-Qing Shen, Yuan-Ming Geng, Ping Liu, Xiang-Yu Huang, Shu-Yi Li, Chun-Dong Liu, Zheng Zhou, Ping-Ping Xu
The present study aimed to investigate the role of magnitude in adaptive response of osteoblasts exposed to compressive stress. Murine primary osteoblasts and MC3T3-E1 cells were exposed to compressive stress (0, 1, 2, 3, 4, and 5 g/cm(2)) in 3D culture. Cell viability was evaluated, and expression levels of Runx2, Alp, Ocn, Rankl, and Opg were examined. ALP activity in osteoblasts and TRAP activity in RAW264.7 cells co-cultured with MC3T3-E1 cells were assayed. Results showed that compressive stress within 5...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28315446/targeted-delivery-of-morin-a-dietary-bioflavanol-encapsulated-mannosylated-liposomes-to-the-macrophages-of-adjuvant-induced-arthritis-rats-inhibits-inflammatory-immune-response-and-osteoclastogenesis
#5
Farhath Sultana, Manoj Kumar Neog, MahaboobKhan Rasool
The purpose of the study was to develop a liposomal drug delivery system for morin, a dietary polyphenol, in order to target the synovial macrophages and investigate the remission of disease severity in the adjuvant-induced arthritic (AIA) rats. To do so, mannose decorated liposomal morin (ML-Morin) was prepared using the thin film hydration method and the physicochemical properties were characterized. The particle size and zeta potential of liposomal morin (L-Morin) was found to be 127.9nm ± 2.6 and -24.5mV ± 0...
March 14, 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28303296/sortase-a-aided-escherichia-coli-expression-system-for-functional-osteoprotegerin-cysteine-rich-domain
#6
Mengmeng Jin, Yuan Chen, Yunfeng Zhao, Luyang Che, Yanyan Ma, Jingzhe Li, Yi Wang, Hua Tao, Juan Ma, Bing Pan, Changzhen Liu, Peng Huang
As a natural inhibitor of the receptor activator of nuclear factor-кB ligand (RANKL), osteprotegerin (OPG) is considered a promising treatment for metabolic bone diseases. Typical approaches for preparing recombinant OPG or its derivatives employ eukaryotic expression systems. Due to the advantages of a prokaryotic expression system, which include its convenience, low cost, and abundant production, in this study, we establish a strategy for preparing functional OPG using the Escherichia coli expression system...
March 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28302039/bone-degeneration-inflammation-and-secondary-complications-of-arthritis-potential-targets-and-their-natural-inhibitors
#7
Mahadevappa Hemshekhar, Ram M Thushara, Somanatahpura K NaveenKumar, Paul Manoj, Mahalingam S Sundaram, Kempaiah Kemparaju, Kesturu S Girish
Arthritis is marked with joint deterioration that affects articular cartilage and subchondral bone. Though cartilage degradation is a major damage during arthritis, the subsequent bone loss cannot be neglected. Progress in the arthritis research has identified clinical importance of bone erosion in destructive arthritis. Studies have showed the key role played by osteoclasts and receptor activator of nuclear factor kappaB ligand (RANKL) signaling in bone erosion. Cathepsins and tartrate resistant acid phosphatase (TRAP) are considered key enzymatic factors contributing to bone erosion...
March 15, 2017: Mini Reviews in Medicinal Chemistry
https://www.readbyqxmd.com/read/28286757/effect-of-tumor-necrosis-factor-inhibitor-therapy-on-osteoclasts-precursors-in-rheumatoid-arthritis
#8
Inês P Perpétuo, Joana Caetano-Lopes, Ana Maria Rodrigues, Raquel Campanilho-Marques, Cristina Ponte, Helena Canhão, Mari Ainola, João E Fonseca
Objective. Tumor necrosis factor (TNF) increases circulating osteoclast (OC) precursors numbers by promoting their proliferation and differentiation. The aim of this study was to assess the effect of TNF inhibitors (TNFi) on the differentiation and activity of OC in rheumatoid arthritis (RA) patients. Methods. Seventeen RA patients treated with TNFi were analyzed at baseline and after a minimum follow-up period of 6 months. Blood samples were collected to assess receptor activator of nuclear factor kappa-B ligand (RANKL) surface expression on circulating leukocytes and frequency and phenotype of monocyte subpopulations...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28285639/denosumab-for-the-treatment-of-adult-multisystem-langerhans-cell-histiocytosis
#9
Polyzois Makras, Marina Tsoli, Athanasios D Anastasilakis, Maria Thanou, Gregory Kaltsas
PURPOSE: An etiological treatment is currently lacking for Langerhans Cell Histiocytosis (LCH). Receptor activator of nuclear factor κB ligand (RANKL) appears to play a central role in the lesional immunological process inducing compensatory osteoprotegerin (OPG) activation. In a preliminary study we aimed to evaluate for the first time the use of denosumab, a RANKL inhibitor, as a targeted treatment strategy in LCH in order to support and enhance endogenous OPG action in order to control or alter the lesional immunological process...
April 2017: Metabolism: Clinical and Experimental
https://www.readbyqxmd.com/read/28284856/magnetic-resonance-imaging-findings-in-children-with-spasmus-nutans
#10
Meredith Bowen, Jason Peragallo, Stephen F Kralik, Andrea Poretti, Thierry A G M Huisman, Bruno P Soares
BACKGROUND: Spasmus nutans (SN) is a rare pediatric ophthalmologic syndrome characterized by nystagmus, head bobbing, and abnormal head positioning. Historically, SN has been associated with underlying optic pathway gliomas (OPG); however, evidence of this association is based primarily on a small number of isolated case reports. Prior retrospective analyses have found the rate of OPG to be <2%, but these studies only intermittently used neuroimaging with computed tomography, which has limited sensitivity for detection of small lesions in the optic pathway...
March 8, 2017: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/28283388/psoralidin-a-prenylated-coumestan-as-a-novel-anti-osteoporosis-candidate-to-enhance-bone-formation-of-osteoblasts-and-decrease-bone-resorption-of-osteoclasts
#11
Yuankun Zhai, Yingying Li, Yanping Wang, Jiawei Cui, Kun Feng, Xijian Kong, Li Chen
Traditional Chinese medicines (TCM) have been proven to prevent osteoporosis, but their clinical applications are not widely recognized due to their complicated ingredients. Psoralidin, a prenylated coumestan, has been reported to prevent bone loss of ovariectomized rats, but detailed mechanisms are still not clear. In current study, we found that both psoralidin and coumestrol promoted osteoblast proliferation and differentiation, as evidenced by improvements in cell proliferation and alkaline phosphatase activity; increased formation of ALP colonies and calcified nodules; enhanced secretion of collagen-I, BMP-2, osteocalcin and osteopontin; and stimulation of the expression of IGF-1, β-catenin, Runx-2, Osterix, and OPG, as well as the mRNA ratio of OPG/RANKL, while significantly decreasing the expression of RANKL...
March 8, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28279156/premature-atherosclerosis-in-children-with-beta-thalassemia-major-new-diagnostic-marker
#12
Laila M Sherief, Osama Dawood, Adel Ali, Hanan S Sherbiny, Naglaa M Kamal, Mohamed Elshanshory, Osama Abd Alazez, Mohamed Abd Alhady, Mohamed Nour, Wesam A Mokhtar
BACKGROUND: Early vascular alteration, atherosclerosis and coronary artery disease have emerged as important cardiovascular complications among beta-thalassemia major (B-TM) patients. The aims of the current study were to assess the prevalence of premature atherosclerosis among our B-TM patients, and to investigate the diagnostic value of serum Osteoprotegerin assay as an early biomarker for atherosclerosis. METHODS: This cross-sectional study was conducted at Hematology unit - Pediatric Department, Zagazig University Children Hospital- Egypt in the period from March 2014 to March 2015...
March 9, 2017: BMC Pediatrics
https://www.readbyqxmd.com/read/28278513/il-6-enhances-osteocyte-mediated-osteoclastogenesis-by-promoting-jak2-and-rankl-activity-in-vitro
#13
Qing Wu, Xiaokang Zhou, Danqing Huang, Yingchen Ji, Feiwu Kang
BACKGROUND/AIMS: Evidence suggests that IL-6 affects bone mass by modulating osteocyte communication towards osteoclasts. However, the mechanism by which IL-6 enhances osteocyte-mediated osteoclastogenesis is unclear. We aimed to investigate the inflammatory factors in serum after orthodontic surgery and their relationship between osteocytes and osteoclasts. METHODS: Serum was obtained from 10 orthognathic surgery patients, and inflammatory factors were detected by ELISA...
March 9, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28276564/dietary-fatty-acids-on-aortic-root-calcification-in-mice-with-metabolic-syndrome
#14
Maria C Naranjo, Beatriz Bermudez, Indara Garcia, Sergio Lopez, Rocio Abia, Francisco J G Muriana, Sergio Montserrat-de la Paz
Metabolic syndrome (MetS) is associated with obesity, dyslipidemia, type 2 diabetes, and chronic low-grade inflammation. The aim of this study was to determine the role of high-fat low-cholesterol diets (HFLCDs) rich in SFAs (HFLCD-SFAs), MUFAs (HFLCD-MUFAs) or MUFAs plus omega-3 long-chain PUFAs (HFLCD-PUFAs) on vascular calcification by the modulation of the RANKL/RANK/OPG system in the aortic roots of Lep(ob/ob)LDLR(-/-) mice. Animals fed with HFLCD-SFAs had increased weight and a greater atheroma plaque size, calcification, and RANKL/CATHK expression in the aortic root than mice on MUFA-enriched diets, with an increasing OPG expression in the aortic roots of the latter...
March 9, 2017: Food & Function
https://www.readbyqxmd.com/read/28274049/use-of-frontal-sinus-and-nasal-septum-pattern-as-an-aid-in-personal-identification-and-determination-of-gender-a-radiographic-study
#15
Kavita Verma, Prashant Nahar, Mohit Pal Singh, Hemant Mathur, S Bhuvaneshwari
INTRODUCTION: Personal identification and gender determination of unknown person has a vital importance in forensic investigation. Human skull radiography is a useful tool in human identification in natural disaster, in any accidents such as fire accident and road traffic accident where body remains become degraded or severely destroyed. AIM: Present study was performed to evaluate the measurement of frontal sinus, uniqueness of various pattern of nasal septum when combined with frontal sinus observed on posterio anterior cephalogram for sex determination as well as personal identification...
January 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28267132/pentraxin3-as-a-new-indicator-of-cardiovascular-related-death-in-patients-with-advanced-chronic-kidney-disease
#16
Marcin Krzanowski, Katarzyna Krzanowska, Mariusz Gajda, Paulina Dumnicka, Artur Dziewierz, Karolina Woziwodzka, Jan A Litwin, Władysław Sułowicz
INTRODUCTION    Pentraxin3 (PTX3) play an important role in the inflammatory response, taking part in recognizing pathogens and damaged tissues. OBJECTIVES    The aim of the study was to assess the relationship between PTX3 levels and all-cause and cardiovascular (CV) mortality in chronic kidney disease (CKD) patients during five-year observation period.  PATIENTS AND METHODS    The study comprised 78 patients (51 hemodialyzed, 27 predialysis). The examined parameters included PTX3, calcium, phosphate, iPTH, interleukin-6 (IL-6), fibroblast growth factor 23 (FGF-23), osteopontin (OPN), osteoprotegerin (OPG), osteocalcin (OC), osteopontin (OPN), osteoprotegerin (OPG), osteocalcin, tumor necrosis factor receptor II (TNF-R II), transforming growth factor-β (TGF-β), hepatocyte growth factor (HGF), stromal cell-derived factor α (SDF1α), and thrombomodulin (TM)...
February 27, 2017: Pol Arch Intern Med
https://www.readbyqxmd.com/read/28263839/osteoprotegerin-regulates-cancer-cell-migration-through-sdf-1-cxcr4-axis-and-promotes-tumour-development-by-increasing-neovascularization
#17
Zahia Benslimane-Ahmim, Jessica Pereira, Anna Lokajczyk, Blandine Dizier, Isabelle Galy-Fauroux, Anne-Marie Fischer, Dominique Heymann, Catherine Boisson-Vidal
We previously reported that OPG is involved in ischemic tissue neovascularization through the secretion of SDF-1 by pretreated-OPG endothelial colony-forming cells (ECFCs). As the vascularization is one of the key factor influencing the tumour growth and cancer cell dissemination, we investigated whether OPG was able to modulate the invasion of human MNNG-HOS osteosarcoma and DU145 prostate cancer cell lines in vitro and in vivo. Cell motility was analysed in vitro by using Boyden chambers. Human GFP-labelled MMNG-HOS cells were inoculated in immunodeficient mice and the tumour nodules formed were then injected with OPG and/or FGF-2, AMD3100 or 0...
March 3, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28261746/bone-resorptive-activity-in-symptomatic-and-asymptomatic-apical-lesions-of-endodontic-origin
#18
M Salinas-Muñoz, M Garrido-Flores, M Baeza, P Huamán-Chipana, J García-Sesnich, R Bologna, R Vernal, M Hernández
OBJECTIVES: The aim of this study is to assess the levels and diagnostic accuracy of a set of bone resorption biomarkers, including TRAP-5, RANKL, and OPG in symptomatic and asymptomatic apical lesions and controls. MATERIALS AND METHODS: Apical tissues from symptomatic and asymptomatic apical periodontitis patients and periodontal ligaments from healthy teeth extracted for orthodontic reasons were processed for tissue homogenization and the levels of TRAP-5, RANKL, and OPG were determined by multiplex assay...
March 6, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28261377/identification-of-patients-affected-by-mitral-valve-prolapse-with-severe-regurgitation-a-multivariable-regression-model
#19
Paola Songia, Benedetta Porro, Mattia Chiesa, Veronika Myasoedova, Francesco Alamanni, Elena Tremoli, Paolo Poggio
Background. Mitral valve prolapse (MVP) is the most common cause of severe mitral regurgitation. Besides echocardiography, up to now there are no reliable biomarkers available for the identification of this pathology. We aim to generate a predictive model, based on circulating biomarkers, able to identify MVP patients with the highest accuracy. Methods. We analysed 43 patients who underwent mitral valve repair due to MVP and compared to 29 matched controls. We assessed the oxidative stress status measuring the oxidized and the reduced form of glutathione by liquid chromatography-tandem mass spectrometry method...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28261305/the-effect-of-osteoprotegerin-on-implant-osseointegration-in-ovariectomized-rats
#20
Yiming Liu, Jing Hu, Biao Liu, Xiliang Jiang, Yunfeng Li
INTRODUCTION: Osteoprotegerin (OPG), the endogenous inhibitor of RANKL, prevents or reverses bone loss in a variety of preclinical models of bone disease. Preclinical studies indicate that osteoporosis significantly impairs implant fixation. This study aims to investigate the role of OPG in implant osseointegration in ovariectomized rats. MATERIAL AND METHODS: Twelve weeks after bilateral ovariectomy, each rat accepted two titanium screws in the proximal tibiae...
March 1, 2017: Archives of Medical Science: AMS
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