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https://www.readbyqxmd.com/read/27904024/cartilage-attachment-morphology-of-the-fetal-cruciate-ligaments-of-the-knee-an-immunohistochemical-study-using-human-fetal-specimens
#1
Masahito Yamamoto, Hiromasa Takada, Takeshi Takayama, Sakura Katsumura, Mayumi Sato, Hiroshi Abe, Gen Murakami, Shin-Ichi Abe
Fetal cruciate ligaments of the knee provide two types of cartilage attachments: to a cartilage fovea or a simple continuation to the perichondrium. To examine a difference in matrix substance between a ligament attachment to the fovea and another attachment to the perichondrium. We histologically observed 12 human fetal femurs in which the posterior (or anterior) cruciate ligament provided a fovea-type (or a perichondrium-type) attachment. Immunohistochemistry of matric substances (aggrecan, versican, tenascin-c) was performed...
2016: Okajimas Folia Anatomica Japonica
https://www.readbyqxmd.com/read/27892493/autologous-temporomandibular-joint-reconstruction-independent-of-exogenous-additives-a-proof-of-concept-study-for-guided-self-generation
#2
Jiao Wei, Tanja Herrler, Dong Han, Kai Liu, Rulin Huang, Markus Guba, Chuanchang Dai, Qingfeng Li
Joint defects are complex and difficult to reconstruct. By exploiting the body's own regenerative capacity, we aimed to individually generate anatomically precise neo-tissue constructs for autologous joint reconstruction without using any exogenous additives. In a goat model, CT scans of the mandibular condyle including articular surface and a large portion of the ascending ramus were processed using computer-aided design and manufacturing. A corresponding hydroxylapatite negative mold was printed in 3D and temporarily embedded into the transition zone of costal periosteum and perichondrium...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27890611/histone-methyltransferase-setdb1-is-indispensable-for-meckel-s-cartilage-development
#3
Kohei Yahiro, Norihisa Higashihori, Keiji Moriyama
The histone methyltransferase Setdb1 represses gene expression by catalyzing lysine 9 of histone H3 trimethylation. Given that the conventional knockout of Setdb1 is embryo-lethal at the implantation stage, its role in craniofacial development is poorly understood. Here, we investigated the role of Setdb1, using conditional knockout mice-in which Setdb1 was deleted in the Meckel's cartilage (Setdb1 CKO)-and the mouse chondrogenic cell line ATDC5-in which Setdb1 was inhibited by siRNA. Deletion of Setdb1 in Meckel's cartilage, the supportive tissue in the embryonic mandible, led to its enlargement, instead of the degeneration that normally occurs...
November 24, 2016: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/27868204/bioengineering-pediatric-scaffold-free-auricular-cartilaginous-constructs
#4
Pedram Akbari, Stephen D Waldman, Sharon L Cushing, Blake C Papsin, Evan J Propst, Joanna F Weber, Herman Yeger, Walid A Farhat
OBJECTIVE: The use of exogenous materials as scaffolds in cartilage tissue engineering has limited the clinical application of resultant constructs due to the risk of postoperative complications. In an effort to minimize such complications, we aim to generate human, scaffold-free auricular cartilaginous constructs. STUDY DESIGN: Laboratory study using pediatric auricular cartilage. METHODS: Remnant, normal pediatric auricular cartilage samples that would have otherwise been discarded were collected and digested to free cells...
November 21, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27853694/autologous-diced-cartilage-in-nasal-septoplasty
#5
Sameh Ibrahim Sersar, Ibrahim Yassin, Mohammed Saad Eldin Aly
Diced rib cartilage is an acceptable option in severe nasal deformities. We present our preliminary experience in KAMC in nasal septoplasties using the autologous diced costal cartilage. This is a retrospective study of the 22 cases who needed the autologous diced costal cartilage in our centre in 4 years. All our patients needed autologous diced rib cartilages. Twelve were wrapped with temporalis fascia, eight needed rectus fascia and perichondrium was used in only 2 cases. The naso-frontal angle for the whole series decreased by a mean of 4...
September 2016: World Journal of Plastic Surgery
https://www.readbyqxmd.com/read/27832272/use-of-costal-perichondrium-as-an-interpositional-graft-for-septal-perforation-closure
#6
Dean M Toriumi, Quintin M Cappelle, Victor Chung
Importance: Closure of septal perforations remains a technically difficult procedure to perform. Objective: To assess the use of costal perichondrium as an interpositional graft to enhance closure of septal perforations using bilateral mucosal flaps and the effectiveness of the procedure. Design, Setting, and Participants: This case series included all 51 consecutive patients presenting with septal perforations from January 1, 2006, to August 31, 2014, at a single institution...
November 10, 2016: JAMA Facial Plastic Surgery
https://www.readbyqxmd.com/read/27830876/positional-changes-in-tendon-insertions-from-bone-to-fascia-development-of-the-pes-anserinus-and-semimembranosus-muscle-insertion-in-human-fetuses
#7
Zhe Wu Jin, Hiroshi Abe, Yu Jin, Shunichi Shibata, Gen Murakami, José Francisco Rodríguez-Vázquez
Development of a long muscle belly in fetal extremities generally requires a definite bony insertion of the long tendon. However, in adults, the pes anserinus and the semimembranosus tendon are inserted into fasciae. Development of fascial insertions in fetuses was investigated by examining serial histological sections obtained from seven fetuses at 8-9 weeks and eight fetuses at 14-16 weeks. The presence of matrix substances and macrophages was also examined by immunohistochemistry. At 8 weeks, the tendons of the semitendinosus, gracilis, sartorius and semimembranosus muscles were straight and inserted into the initial shaft-like proximal end of the tibia on the proximal side of the popliteus muscle...
November 10, 2016: Folia Morphologica (Warsz)
https://www.readbyqxmd.com/read/27806676/the-role-of-cell-seeding-bio-scaffolds-and-the-in-vivo-microenvironment-in-the-guided-generation-of-osteochondral-composite-tissue
#8
Jiao Wei, Tanja Herrler, Kai Liu, Dong Han, Mei Yang, Chuanchang Dai, Qing Feng Li
Tissue engineering based on cell seeding, bio-scaffolds, and growth factors has been widely applied for the reconstruction of tissue defects. Recent progress has fueled in vivo tissue engineering techniques in becoming hot topics in regenerative medicine and reconstructive surgery. To improve the efficacy of tissue engineering, we here investigated the roles of cell seeding, bio-scaffolds, growth factors, and in-vivo microenvironment in tissue regeneration. Bone marrow derived stem cells (BMSCs), allogenic demineralized bone matrix (DBM) as bio-scaffold, and growth factors bone morphogenetic protein 2/ transforming growth factor (BMP-2/TGF-β1), and the in-vivo microenvironment of rib periosteum and perichondrium were used in different combinations for the generation of osteochondral composite tissue...
November 2, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27755426/bone-marrow-stem-cells-and-ear-framework-reconstruction
#9
Hamid Karimi, Seyed-Abolhassan Emami, Mohammad-Kazem Olad-Gubad
BACKGROUND: Repair of total human ear loss or congenital lack of ears is one of the challenging issues in plastic and reconstructive surgery. OBJECTIVE: The aim of the present study was 3D reconstruction of the human ear with cadaveric ear cartilages seeded with human mesenchymal stem cells. METHOD: We used cadaveric ear cartilages with preserved perichondrium. The samples were divided into 2 groups: group A (cartilage alone) and group B (cartilage seeded with a mixture of fibrin powder and mesenchymal stem cell [1,000,000 cells/cm] used and implanted in back of 10 athymic rats)...
October 14, 2016: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/27739349/costal-cartilage-graft-with-perichondrium-a-possible-anti-adhesive-material
#10
Norio Fukuda, Hirotaka Asato, Kohei Umekawa, Goro Takada, Takeshi Kan, Shoichi Sasaki
BACKGROUND: Adhesion occurs as a part of the wound healing process, but it sometimes compromises patients' daily activities. The authors were looking for materials and methods that could prevent adhesion, and noticed that the costal cartilage has possibility. The anti-adhesive property of the costal cartilage was examined histologically. METHODS: Thirty-five patients with microtia who provided consent for participating in this study were enrolled between April 2008 and March 2015...
October 14, 2016: Journal of Plastic Surgery and Hand Surgery
https://www.readbyqxmd.com/read/27734993/cartilage-canals-in-newborn-dogs-histochemical-and-immunohistochemical-findings
#11
A Di Giancamillo, M E Andreis, P Taini, M C Veronesi, M Di Giancamillo, S C Modina
Cartilage canals (CCs) are microscopic structures involved in secondary ossification centers (SOCs) development. The features of CCs were investigated in the humeral and femoral proximal epiphyses of small-sized newborn dogs (from premature to 28 days after birth) with histochemical and immunohistochemical approaches. Masson's Trichrome revealed a ring-shaped area around CCs, which changes in colour from green (immature collagen) to red (mature collagen) as ossification progresses; perichondrium staining always matched the ring colour...
September 15, 2016: European Journal of Histochemistry: EJH
https://www.readbyqxmd.com/read/27734979/the-audiological-and-take-results-of-perichondrium-attached-cartilage-island-graft-in-tympanoplasty-pacit
#12
F Solmaz, D Akduman, M Haksever, E Gündoğdu, M Yanılmaz, A Mescioğlu
Cartilage is one of the most preferable grafts for tympanoplasty (TPL). The anatomical and audiological results and take rates of perichondrium attached cartilage island graft in tympanoplasty (PACIT) are presented herein. One hundred ninety four ears of 191 patients (108 male, 83 female) were evaluated retrospectively in terms of the type of surgery, graft take rate and hearing results. Type I, II, and III TPL were performed in 127 (65.46%), 45 (23.20%), and 22 (11.34%) ears, respectively. The overall mean preoperative pure tone average-air bone gaps (PTA-ABGs) for TPL types were 33...
August 2016: Acta Otorhinolaryngologica Italica
https://www.readbyqxmd.com/read/27680521/nonsurgical-rhinoplasty
#13
Owen N Johnson, Theda C Kontis
"Nonsurgical rhinoplasty" refers to the use of injectable temporary fillers used to augment select areas of the nose to achieve improved appearance or function in select patients. Nonpermanent fillers can be used safely and must be properly chosen based on their properties and the desired effect. In addition to proper patient and product selection, a thorough understanding of nasal anatomy, analysis of the deformity, and recommended injection techniques are absolutely necessary to obtain good outcomes and avoid complications...
October 2016: Facial Plastic Surgery: FPS
https://www.readbyqxmd.com/read/27668792/minimally-invasive-surgery-for-the-treatment-of-hyperacusis
#14
Herbert Silverstein, Rosemary Ojo, Julie Daugherty, Ronen Nazarian, Jack Wazen
OBJECTIVE: To evaluate the efficacy of a minimally invasive surgical procedure in patients with severe hyperacusis. STUDY DESIGN: Prospective, longitudinal design. SETTING: Tertiary referral center. PATIENTS: Adult patients with history of severe hyperacusis. INTERVENTION: Using a transcanal approach, the round and oval window was reinforced with temporalis fascia or tragal perichondrium in six subjects (nine ears) and was subdivided into two groups (unilateral or bilateral reinforcement procedure)...
December 2016: Otology & Neurotology
https://www.readbyqxmd.com/read/27627972/revision-myringoplasty-using-thin-sliced-cartilage-for-postoperative-reperforation-in-patients-with-chronic-otitis-media
#15
Hideaki Suzuki, Hiroki Koizumi, Takuro Kitamura, Takahisa Tabata, Yasuhiro Kise, Koichi Hashida
Myringoplasty is one of the basic procedures in otologic surgery, and is important to achieve good hearing outcome. The temporal fascia is most widely used and considered to be a stable graft in this procedure, although 10-20% of patients develop reperforation after surgery, which is often hard to repair, even by revision surgery. We herein conducted revision myringoplasty using a cartilage graft to repair postoperative reperforation in 7 patients (8 ears) with chronic otitis media. The patients were 3 males and 4 females, aged 13-80 years with an average of 53...
September 2016: Journal of UOEH
https://www.readbyqxmd.com/read/27567383/evaluation-of-the-anatomical-and-auditory-outcomes-of-minimally-invasive-cartilage-myringoplasty-our-technique-and-experience
#16
Eda Simsek, Ozalkan Ozkan, Cüneyt Kucur, Ayşe Carlıoglu
: The purpose of this study was to assess closure rates in tympanic membrane perforations of various dimensions using the tragal cartilage-perichondrium composite graft and its effect on hearing values and also to present our own experiences. MATERIALS AND METHODS: Sixty-one patients presenting to our clinic in 2014-2015 and diagnosed with tympanic membrane perforation were included in the study. Otomicroscopic and otoendoscopic examinations were performed preoperatively and at the 12th month postoperatively...
August 5, 2016: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/27500982/morphogenesis-of-the-middle-ear-ossicles-and-spatial-relationships-with-the-external-and-inner-ears-during-the-embryonic-period
#17
Maimi Ozeki-Satoh, Aoi Ishikawa, Shigehito Yamada, Chigako Uwabe, Tetsuya Takakuwa
We describe the three-dimensional morphogenesis of the middle ear ossicles (MEOs) according to Carnegie stage (CS) in human embryos. Seventeen samples including 33 MEOs from CS18 to 23 were selected from the Kyoto Collection. The primordia of the MEOs and related structures were histologically observed and three-dimensionally reconstructed from digital images. The timing of chondrogenesis was variable among structures. The stapes was recognizable as a vague condensation of the mesenchymal cells in all samples from CS18, whereas the malleus and incus were recognizable at CS19...
October 2016: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/27498042/distribution-of-type-vi-collagen-in-association-with-osteoblast-lineages-in-the-groove-of-ranvier-during-rat-postnatal-development
#18
Yukihiro Kohara, Satoshi Soeta, Yayoi Izu, Kiyotaka Arai, Hajime Amasaki
In the groove of Ranvier (GOR), osteoblast lineages form bone bark, which develops into endosteal cortical bone. This ossification process is thought to be regulated by the microenvironment in the GOR. Type VI collagen (Col VI), an extracellular matrix (ECM) protein found in the periosteum/perichondrium, mediates osteoblast differentiation via the cell-surface receptor neural/glial antigen 2 (NG2) chondroitin sulfate proteoglycan. In order to clarify the function of Col VI during osteoblast differentiation in the GOR, in the present study, we examined the distribution of Col VI and osteoblast lineages expressing NG2 in the rat tibia proximal end during postnatal growing periods by immunohistochemistry...
November 2016: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/27485368/comparison-of-results-of-graft-uptake-using-tragal-cartilage-perichondrium-composite-graft-versus-temporalis-fascia-in-patients-undergoing-surgery-for-chronic-otitis-media-squamous-type
#19
S Khalilullah, Shankar P Shah, D Yadav, R P Shrivastav, H Bhattarai
BACKGROUND: To assess, analyze and compare the results of graft uptake using Tragal Cartilage-Perichondrium Composite (TCPC) graft with Temporalis Fascia (TF) graft in patients who undergoing surgery for chronic otitis media - squamous type. METHODS: Patients aged 13 years and above with diagnosis of chronic otitis media - squamous type undergoing modified radical mastoidectomy, either primary or revision surgery with grafting of tympanic membrane (TM) and patients undergoing excision of postero-superior retraction pocket (PSRP) were included in this study...
2016: Head & Face Medicine
https://www.readbyqxmd.com/read/27471470/microrna-profiling-during-craniofacial-development-potential-roles-for-mir23b-and-mir133b
#20
Hai-Lei Ding, Joan E Hooper, Peter Batzel, B Frank Eames, John H Postlethwait, Kristin B Artinger, David E Clouthier
Defects in mid-facial development, including cleft lip/palate, account for a large number of human birth defects annually. In many cases, aberrant gene expression results in either a reduction in the number of neural crest cells (NCCs) that reach the frontonasal region and form much of the facial skeleton or subsequent failure of NCC patterning and differentiation into bone and cartilage. While loss of gene expression is often associated with developmental defects, aberrant upregulation of expression can also be detrimental...
2016: Frontiers in Physiology
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