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https://www.readbyqxmd.com/read/28196691/healing-of-a-large-long-bone-defect-through-serum-free-in%C3%A2-vitro-priming-of%C3%A2-human-periosteum-derived-cells
#1
Johanna Bolander, Wei Ji, Jeroen Leijten, Liliana Moreira Teixeira, Veerle Bloemen, Dennis Lambrechts, Malay Chaklader, Frank P Luyten
Clinical translation of cell-based strategies for regenerative medicine demands predictable in vivo performance where the use of sera during in vitro preparation inherently limits the efficacy and reproducibility. Here, we present a bioinspired approach by serum-free pre-conditioning of human periosteum-derived cells, followed by their assembly into microaggregates simultaneously primed with bone morphogenetic protein 2 (BMP-2). Pre-conditioning resulted in a more potent progenitor cell population, while aggregation induced osteochondrogenic differentiation, further enhanced by BMP-2 stimulation...
February 7, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28196155/subperiosteal-minimally-invasive-aesthetic-ridge-augmentation-technique-smart-a-new-standard-for-bone-reconstruction-of-the-jaws
#2
Ernesto A Lee
Traditional guided bone regeneration techniques include flap mobilization and placement of a bone graft, often with the use of space-maintaining devices and cell-occlusive membranes. This approach is associated with frequent complications that negatively affect the outcome of the augmentation and the peri-implant soft tissue esthetics. Although current tunneling techniques have focused on periodontal soft tissue applications, earlier publications described their use for horizontal augmentation of mandibular posterior edentulous ridges in full-denture patients...
March 2017: International Journal of Periodontics & Restorative Dentistry
https://www.readbyqxmd.com/read/28170186/live-tissue-imaging-to-elucidate-mechanical-modulation-of-stem-cell-niche-quiescence
#3
Nicole Y C Yu, Connor A O'Brien, Iveta Slapetova, Renee M Whan, Melissa L Knothe Tate
The periosteum, a composite cellular connective tissue, bounds all nonarticular bone surfaces. Like Velcro, collagenous Sharpey's fibers anchor the periosteum in a prestressed state to the underlying bone. The periosteum provides a niche for mesenchymal stem cells. Periosteal lifting, as well as injury, causes cells residing in the periosteum (PDCs) to change from an immobile, quiescent state to a mobile, active state. The physical cues that activate PDCs to home to and heal injured areas remain a conundrum...
January 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28167822/accelerating-corrosion-of-pure-magnesium-co-implanted-with-titanium-in-vivo
#4
Peng Hou, Pei Han, Changli Zhao, Hongliu Wu, Jiahua Ni, Shaoxiang Zhang, Jingyi Liu, Yuanzhuang Zhang, Haidong Xu, Pengfei Cheng, Shen Liu, Yufeng Zheng, Xiaonong Zhang, Yimin Chai
Magnesium is a type of reactive metal, and is susceptible to galvanic corrosion. In the present study, the impact of coexistence of Ti on the corrosion behavior of high purity Mg (HP Mg) was investigated both in vitro and in vivo. Increased corrosion rate of HP Mg was demonstrated when Mg and Ti discs were not in contact. The in vivo experiments further confirmed accelerating corrosion of HP Mg screws when they were co-implanted with Ti screws into Sprague-Dawley rats' femur, spacing 5 and 10 mm. Micro CT scan and 3D reconstruction revealed severe corrosion morphology of HP Mg screws...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28162855/histopathological-and-scanning-electron-microscopy-findings-of-retrieved-porous-polyethylene-implants
#5
S-Y Choi, H-I Shin, T-Y Kwon, T-G Kwon
Porous polyethylene (PPE) implants are biocompatible alloplastic materials commonly used for facial augmentation. However, the effect of sub-periosteal PPE application on the surrounding tissues has not been analyzed clearly. This report documents the case of a 22-year-old woman who underwent peri-alar augmentation with PPE to improve midface retrusion. Although no infection or inflammation occurred at the surgical site, the patient requested removal of the PPE implant for aesthetic reasons alone at 1 year after the surgery...
February 2, 2017: International Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/28162164/-repair-mechanisms-of-the-wounds-with-exposed-bone-structures-using-an-artificial-dermis
#6
X Chen, C Wang, C Zhang, X Huang, H S Cao
Objective: To investigate the repair mechanisms of the wounds with bone exposed by artificial dermis transplantation. Methods: Seventy two rabbits were randomly divided into 3 groups of 24 rabbits, the wound model was made on the top of head. In the skin defect group (SD group), skin was removed and skull periosteum was retained. In the periosteal defect group (PD group), the skin and skull periosteum were both removed, and the skull was exposed. In the skull burn group (SB group), after the periosteum was removed, the exposed skull bone was burned out with electric iron to cause skull surface necrosis, then the artificial dermis transplantation were performed in each group...
January 24, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
https://www.readbyqxmd.com/read/28160906/thumb-reconstruction-with-thin-proximal-ulnar-perforator-free-flap
#7
REVIEW
Mario Cherubino, Martina Corno, Luigi Valdatta, Roberto Adani
Soft tissue defects of the thumb with exposure of tendons, periosteum, or neurovascular bundles require a complex reconstruction aimed at restoring both skin coverage and the essential function of pinching and manipulation. When large defects are involved, a free tissue transfer is indicated. The proximal ulnar perforator flap represents an interesting solution without the drawbacks of other more widespread free flaps. This report describes the case of a patient with a squamous cell carcinoma of the thumb that required circumferential resection of the soft tissue of the distal phalanx and the interphalangeal joint...
February 2017: Journal of Hand Surgery
https://www.readbyqxmd.com/read/28129512/long-term-outcomes-of-autologous-chondrocyte-implantation-in-adolescent-patients
#8
Takahiro Ogura, Tim Bryant, Tom Minas
BACKGROUND: Treating symptomatic articular cartilage lesions is challenging, especially in adolescent patients, because of longer life expectancies and higher levels of functional activity. For this population, long-term outcomes after autologous chondrocyte implantation (ACI) remain to be determined. PURPOSE: To evaluate long-term outcomes in adolescents after ACI using survival analyses, validated outcome questionnaires, and standard radiographs. STUDY DESIGN: Case series; Level of evidence, 4...
January 1, 2017: American Journal of Sports Medicine
https://www.readbyqxmd.com/read/28124109/decompression-endoscopic-surgery-for-frontal-secondary-headache-attributed-to-supraorbital-and-supratrochlear-nerve-entrapment-a-comprehensive-review
#9
REVIEW
Boris Filipović, J Alexander de Ru, Rick van de Langenberg, Pepijn A Borggreven, Zdravko Lacković, Peter J F M Lohuis
In the last decade, a new surgical treatment modality was developed for frontal secondary headache, based on the assumption that the trigger of this pain entity is the entrapment of peripheral sensory nerves. The surgery entails a procedure, where an endoscopic approach is used to decompress the supraorbital and supratrochlear nerve branches, which are entrapped by the periosteum in the region of the corrugator supercilii muscle. Candidates for the surgery define their headache as moderate to severe persistent daily pressure or tension, localized in the frontal area, sometimes accompanied by symptoms of nausea and photophobia mimicking a primary headache-migraine...
January 25, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28109101/-fgf-2-pela-bmp-2-microcapsule-scaffold-promotes-osteogenic-differentiation-of-rat-periosteum-derived-stem-cells-in-vitro
#10
Jie Yin, Su-Jun Qiu, Jun-Huai Gao, Sheng-Li Zhao, Shao-Xiong Min
OBJECTIVE: To observe the effect of a microencapsule scaffold capable of sustained release of fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) in promoting the osteogenic differentiation of rat periosteum-derived stem cells (PDSCs) in vitro. METHODS: PDSCs from 4-week-old SD rats, after identification of the surface markers using flow cytometry, were induced to differentiate into osteoblast, chondroblast, and adipocyte lineages. The differentiated cells were verified by staining with Alizarin red, toluidine blue, alcian blue, oil red O and by immunofluorescence assay...
January 20, 2017: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/28105092/serum-n-terminal-telopeptide-of-type-i-collagen-as-an-early-marker-of-fracture-nonunion-in-rabbits
#11
Jian-Ping Lin, Zhan-Jun Shi, Ning-Jiang Shen, Jian Wang, Zao-Min Li, Jun Xiao
The aim of the present study was to establish an experimental animal model of fracture nonunion, and to investigate the changes in serum biomarker concentrations in fracture nonunion. A total of 20 purebred New Zealand rabbits were divided into two group: A bone defect group and a bone fracture group. In the bone defect group, a 15-mm section of bone (including the periosteum) was removed from the mid-radius, and the medullary cavities were closed with bone wax. In the bone fracture group, the mid-radius was fractured...
December 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28100683/impact-of-microscopic-orbital-periosteum-invasion-in-orbital-preservation-surgery
#12
Kenya Kobayashi, Taisuke Mori, Fumihiko Matsumoto, Naoya Murakami, Masanori Teshima, Masahiko Fukasawa, Yoshifumi Matsumoto, Satoko Matsumura, Jun Itami, Masahiro Asai, Seiichi Yoshimoto
OBJECTIVE: The orbital periosteum is considered to be a barrier to tumor spread; however, it is difficult to evaluate microscopic tumor spread during surgery. This study aimed to assess the impact of pathological status in orbital preservation surgery. METHODS: We retrospectively analyzed the 3-year local control rate and treatment outcomes of patients with malignant tumors invading the orbit who were treated between 2006 and 2012. RESULTS: In total, 27 patients were reviewed over a median follow-up period of 36 months...
January 17, 2017: Japanese Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28094420/evaluation-of-the-optimal-plate-position-for-the-fixation-of-supraglenoid-tubercle-fractures-in-warmbloods
#13
Sina Frei, Hans Geyer, Seamus Hoey, Anton E Fuerst, Andrea S Bischofberger
OBJECTIVES: To determine scapular cortex thickness, distal scapular bone density and describe the exact suprascapular nerve course to evaluate the best plate position for the fixation of supraglenoid tubercle fractures in horses. METHODS: Twelve equine cadaveric shoulders were examined with computed tomography. Computed tomography morphometry and density measurements (Hounsfield units [HU]) of the scapula were recorded. Statistical comparisons were made between the cranial and caudal aspects of the scapula...
January 17, 2017: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
https://www.readbyqxmd.com/read/28092776/periosteum-tissue-engineering-in-an-orthotopic-in%C3%A2-vivo-platform
#14
J G Baldwin, F Wagner, L C Martine, B M Holzapfel, C Theodoropoulos, O Bas, F M Savi, C Werner, E M De-Juan-Pardo, D W Hutmacher
The periosteum plays a critical role in bone homeostasis and regeneration. It contains a vascular component that provides vital blood supply to the cortical bone and an osteogenic niche that acts as a source of bone-forming cells. Periosteal grafts have shown promise in the regeneration of critical size defects, however their limited availability restricts their widespread clinical application. Only a small number of tissue-engineered periosteum constructs (TEPCs) have been reported in the literature. A current challenge in the development of appropriate TEPCs is a lack of pre-clinical models in which they can reliably be evaluated...
March 2017: Biomaterials
https://www.readbyqxmd.com/read/28079807/a-case-report-distal-humeral-diaphyseal-fracture-in-a-child
#15
Yuji Tomori, Takuya Sawaizumi, Mitsuhiko Nanno, Shinro Takai
RATIONALE: Distal humeral diaphyseal fractures are much less common than supracondylar humeral fractures. The triangular shape and thinner periosteum in the diaphyseal region than in the supracondylar region of the distal diaphysis makes the treatment of diaphyseal fractures difficult. Nonoperative treatment for this fracture is challenging and troublesome; thus, open reduction and internal fixation (OR/IF) is generally recommended. PATIENT CONCERNS: We herein report a distal humeral diaphyseal fracture in a child...
January 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28074876/scale-up-of-nature-s-tissue-weaving-algorithms-to-engineer-advanced-functional-materials
#16
Joanna L Ng, Lillian E Knothe, Renee M Whan, Ulf Knothe, Melissa L Knothe Tate
We are literally the stuff from which our tissue fabrics and their fibers are woven and spun. The arrangement of collagen, elastin and other structural proteins in space and time embodies our tissues and organs with amazing resilience and multifunctional smart properties. For example, the periosteum, a soft tissue sleeve that envelops all nonarticular bony surfaces of the body, comprises an inherently "smart" material that gives hard bones added strength under high impact loads. Yet a paucity of scalable bottom-up approaches stymies the harnessing of smart tissues' biological, mechanical and organizational detail to create advanced functional materials...
January 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28070629/-susceptibility-to-infections-and-behavior-of-stainless-steel-comparison-with-titanium-implants-in-traumatology
#17
Patrick Haubruck, Gerhard Schmidmaier
BACKGROUND: Despite modern treatment options, implant-associated infections (IAI) remain a severe and challenging complication in the treatment of trauma patients. Almost 30 years after the introduction of implants made of titanium alloy into the treatment of trauma patients, there is still no uniform consensus regarding the clinical benefit of titanium alloy in the context of patients with IAI. OBJECTIVE: We sought to determine if implants made of titanium alloy have been proven to be less susceptible regarding IAI in contrast to implants made of stainless steel...
January 9, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28054920/evaluation-of-honey-as-a-topical-therapy-for-intraoral-wound-healing-in-rats
#18
Goli Chamani, Mohammad Reza Zarei, Mitra Mehrabani, Ali Mehdavinezhad, Mehrangiz Vahabian, Fatemah Ahmadi-Motamayel
OBJECTIVE: Honey is one of the oldest known medicines. Its medical and therapeutic importance has been recently rediscovered. Honey is an effective treatment for infected wounds and ulcers. The aim of this study was to determine the effect of honey as a topical therapy for intraoral wound healing in rats. MATERIALS AND METHODS: Thirty-two male rats were divided into experimental and control groups (consisting of 16 rats, 4 animals in each group). A 2-mm mucosal defect was made to the depth of the periosteum using punch biopsy...
December 29, 2016: Wounds: a Compendium of Clinical Research and Practice
https://www.readbyqxmd.com/read/28042273/gingival-crevicular-fluid-periostin-levels-in-chronic-periodontitis-patients-following-nonsurgical-periodontal-treatment-with-low-level-laser-therapy
#19
Dhanangchaayan Kumaresan, Aruna Balasundaram, Vanaja Krishna Naik, Deva Priya Appukuttan
OBJECTIVE: Periostin is a matricellular protein highly expressed in periosteum, periodontal ligament and is essential for tissue integrity and maturation. It plays a role in collagen fibrillogenesis and is downregulated in periodontal disease. Biostimulation utilizing low-level laser therapy (LLLT) influences periodontal ligament fibroblast proliferation. This study was conducted with the objective of estimating periostin levels in chronic periodontitis (CP) patients following LLLT as an adjunct to root surface debridement (RSD)...
October 2016: European Journal of Dentistry
https://www.readbyqxmd.com/read/28040084/do-skeletal-muscle-mscs-in-humans-contribute-to-bone-repair-a-systematic-review
#20
Heather Owston, Peter V Giannoudis, Elena Jones
Mesenchymal stem cells (MSC) from bone marrow and periosteum are known to be heavily involved in fracture repair and bone regeneration is thought to be impaired when the surrounding skeletal muscle is damaged. Recent literature from mouse in vivo models suggest that cells originating from skeletal muscle can occupy a fracture callus during open fracture repair when periosteum is compromised. This systematic review set out to ascertain whether there are MSCs residing in human skeletal muscle and whether cells from human skeletal muscle are capable of forming bone in vitro and in vivo...
December 2016: Injury
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