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https://www.readbyqxmd.com/read/27848974/differential-expression-of-tgf-%C3%AE-superfamily-members-and-role-of-smad1-5-9-signalling-in-chondral-versus-endochondral-chondrocyte-differentiation
#1
Verena Dexheimer, Jessica Gabler, Katharina Bomans, Tanja Sims, Georg Omlor, Wiltrud Richter
Proteins of the transforming-growth-factor-β (TGF-β)-superfamily have a remarkable ability to induce cartilage and bone and the crosstalk of TGF-β - and BMP-signalling pathways appears crucial during chondrocyte development. Aim was to assess the regulation of TGF-β-superfamily members and of Smad2/3- and Smad1/5/9-signalling during endochondral in vitro chondrogenesis of mesenchymal stromal cells (MSC) relative to chondral redifferentiation of articular chondrocytes (AC) to adjust chondrocyte development of MSC towards a less hypertrophic phenotype...
November 16, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27818692/implant-composed-of-demineralized-bone-and-mesenchymal-stem-cells-genetically-modified-with-adbmp2-adbmp7-for-the-regeneration-of-bone-fractures-in-ovis-aries
#2
Adelina A Hernandez-Hurtado, Gissela Borrego-Soto, Ivan A Marino-Martinez, Jorge Lara-Arias, Viktor J Romero-Diaz, Adalberto Abrego-Guerra, Jose F Vilchez-Cavazos, Guillermo Elizondo-Riojas, Herminia G Martinez-Rodriguez, Marcela A Espinoza-Juarez, Gloria C Lopez-Romero, Alejandro Robles-Zamora, Oscar F Mendoza Lemus, Rocio Ortiz-Lopez, Augusto Rojas-Martinez
Adipose-derived mesenchymal stem cells (ADMSCs) are inducible to an osteogenic phenotype by the bone morphogenetic proteins (BMPs). This facilitates the generation of implants for bone tissue regeneration. This study evaluated the in vitro osteogenic differentiation of ADMSCs transduced individually and in combination with adenoviral vectors expressing BMP2 and BMP7. Moreover, the effectiveness of the implant containing ADMSCs transduced with the adenoviral vectors AdBMP2/AdBMP7 and embedded in demineralized bone matrix (DBM) was tested in a model of tibial fracture in sheep...
2016: Stem Cells International
https://www.readbyqxmd.com/read/27815159/expression-and-functional-role-of-bone-morphogenetic-proteins-bmps-in-cyclical-corpus-luteum-in-buffalo-bubalus-bubalis
#3
G Rajesh, Avishek Paul, S R Mishra, Jaya Bharati, Nipuna Thakur, Tanmay Mondal, Sanjhali Soren, S Harikumar, K Narayanan, V S Chouhan, Sadhan Bag, B C Das, G Singh, V P Maurya, G Taru Sharma, Mihir Sarkar
The role of growth factors in the modulation of ovarian function is an interesting area of research in reproductive biology. Recently, we have shown the expression and role of IGF, EGF, VEGF and FGF in the follicle and CL. Here, we report the presence of Bone Morphogenetic Proteins (BMPs) and their functional receptors in the corpus luteum (CL) of buffalo. The bubaline CL was classified into four stages according to the morphology and progesterone (P4) concentration. The qPCR, immunoblot and immunohistochemistry studies revealed that BMP2 and BMP Receptors (BMPR1A, BMPR1B and BMPR2) were significantly upregulated during the mid stage whereas BMP4 and BMP7 were upregulated during the early stage of CL (P<0...
November 1, 2016: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/27803898/candidate-gene-expression-in-bos-indicus-ovarian-tissues-prepubertal-and-postpubertal-heifers-in-diestrus
#4
Mayara Morena Del Cambre Amaral Weller, Marina Rufino S Fortes, Laercio R Porto-Neto, Matthew Kelly, Bronwyn Venus, Lisa Kidd, João Paulo Arcelino do Rego, Sophia Edwards, Gry B Boe-Hansen, Emily Piper, Sigrid A Lehnert, Simone Eliza Facioni Guimarães, Stephen Stewart Moore
Growth factors such as bone morphogenetic proteins 6, 7, 15, and two isoforms of transforming growth factor-beta (BMP6, BMP7, BMP15, TGFB1, and TGFB2), and insulin-like growth factor system act as local regulators of ovarian follicular development. To elucidate if these factors as well as others candidate genes, such as estrogen receptor 1 (ESR1), growth differentiation factor 9 (GDF9), follicle-stimulating hormone receptor (FSHR), luteinizing hormone receptor (LHR), bone morphogenetic protein receptor, type 2 (BMPR2), type 1 insulin-like growth factor receptor (IGFR1), and key steroidogenic enzymes cytochrome P450 aromatase and 3-β-hydroxysteroid dehydrogenase (CYP19A1 and HSD3B1) could modulate or influence diestrus on the onset of puberty in Brahman heifers, their ovarian mRNA expression was measured before and after puberty (luteal phase)...
2016: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/27766493/ccn2-reduction-mediates-protective-effects-of-bmp7-treatment-in-obstructive-nephropathy
#5
Lucas L Falke, Jan Willem Leeuwis, Karen M Lyons, Christine L Mummery, Tri Q Nguyen, Roel Goldschmeding
Treatment with rhBMP7 exerts profound protective effects in a wide variety of experimental models of renal disease. However, little is known about how these protective effects are mediated, and which cells in the kidney are targeted by exogenous rhBMP7 treatment. To determine if rhBMP7 increases glomerular and tubulointerstitial canonical BMP signaling, we performed Unilateral Ureteral Obstruction (UUO, a widely used obstructive nephropathy model) in mice reporting transcriptional activity downstream of canonical BMP signaling by the expression of GFP under the BMP Responsive Element of the Id1 promoter (BRE:gfp mice)...
October 20, 2016: Journal of Cell Communication and Signaling
https://www.readbyqxmd.com/read/27752241/tcf4-controls-neuronal-migration-of-the-cerebral-cortex-through-regulation-of-bmp7
#6
Tianda Chen, Qinwei Wu, Yang Zhang, Tianlan Lu, Weihua Yue, Dai Zhang
Background: Transcription factor 4 (TCF4) is found to be associated with schizophrenia. TCF4 mutations also cause Pitt-Hopkins Syndrome, a neurodevelopmental disorder associated with severe mental retardation. However, the function of TCF4 during brain development remains unclear. Results: Here, we report that Tcf4 is expressed in the developing cerebral cortex. In utero suppression of Tcf4 arrested neuronal migration, leading to accumulation of ectopic neurons in the intermediate zone. Knockdown of Tcf4 impaired leading process formation...
2016: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/27713089/the-fibrodysplasia-ossificans-progressiva-fop-mutation-p-r206h-in-acvr1-confers-an-altered-ligand-response
#7
Laura Hildebrand, Katja Stange, Alexandra Deichsel, Manfred Gossen, Petra Seemann
Patients with Fibrodysplasia Ossificans Progressiva (FOP) suffer from ectopic bone formation, which progresses during life and results in dramatic movement restrictions. Cause of the disease are point mutations in the Activin A receptor type 1 (ACVR1), with p.R206H being most common. In this study we compared the signalling responses of ACVR1(WT) and ACVR1(R206H) to different ligands. ACVR1(WT), but not ACVR1(R206H) inhibited BMP signalling of BMP2 or BMP4 in a ligand binding domain independent manner. Likewise, the basal BMP signalling activity of the receptor BMPR1A or BMPR1B was inhibited by ACVR1(WT), but enhanced by ACVR1(R206H)...
October 4, 2016: Cellular Signalling
https://www.readbyqxmd.com/read/27709558/dna-copy-number-alterations-gene-expression-changes-and-disease-free-survival-in-patients-with-colorectal-cancer-a-10-year-follow-up
#8
Elisabetta Bigagli, Carlotta De Filippo, Cinzia Castagnini, Simona Toti, Francesco Acquadro, Francesco Giudici, Marilena Fazi, Piero Dolara, Luca Messerini, Francesco Tonelli, Cristina Luceri
BACKGROUND: DNA copy number alterations (CNAs) and gene expression changes have amply been encountered in colorectal cancers (CRCs), but the extent at which CNAs affect gene expression, as well as their relevance for tumor development, are still poorly defined. Here we aimed at assessing the clinical relevance of these parameters in a 10 year follow-up study. METHODS: Tumors and normal adjacent colon mucosa, obtained at primary surgery from 21 CRC patients, were subjected to (i) high-resolution array CGH (a-CGH) for the detection of CNAs and (ii) microarray-based transcriptome profiling for the detection of gene expression (GE) changes...
October 5, 2016: Cellular Oncology (Dordrecht)
https://www.readbyqxmd.com/read/27696331/tgf-beta-receptor-mediated-telomerase-inhibition-telomere-shortening-and-breast-cancer-cell-senescence
#9
Lucy Cassar, Craig Nicholls, Alex R Pinto, Ruping Chen, Lihui Wang, He Li, Jun-Ping Liu
Human telomerase reverse transcriptase (hTERT) plays a central role in telomere lengthening for continuous cell proliferation, but it remains unclear how extracellular cues regulate telomerase lengthening of telomeres. Here we report that the cytokine bone morphogenetic protein-7 (BMP7) induces the hTERT gene repression in a BMPRII receptor- and Smad3-dependent manner in human breast cancer cells. Chonic exposure of human breast cancer cells to BMP7 results in short telomeres, cell senescence and apoptosis...
September 30, 2016: Protein & Cell
https://www.readbyqxmd.com/read/27693382/effects-of-physical-exercise-on-myokines-expression-and-brown-adipose-like-phenotype-modulation-in-rats-fed-a-high-fat-diet
#10
Sílvia Rocha-Rodrigues, Amaia Rodríguez, Alexandra M Gouveia, Inês O Gonçalves, Sara Becerril, Beatriz Ramírez, Jorge Beleza, Gema Frühbeck, António Ascensão, José Magalhães
AIMS: Exercise-stimulated myokine secretion into circulation may be related with browning in white adipose tissue (WAT), representing a positive metabolic effect on whole-body fat mass. However, limited information is yet available regarding the impact of exercise on myokine-related modulation of adipocyte phenotype in WAT from obese rats. MAIN METHODS: Sprague-Dawley rats (n=60) were divided into sedentary and voluntary physical activity (VPA) groups and fed with standard (35kcal% fat) or high-fat (HFD, 71kcal% fat)-isoenergetic diets...
November 15, 2016: Life Sciences
https://www.readbyqxmd.com/read/27679685/roles-and-regulation-of-bone-morphogenetic-protein-7-in-kidney-development-and-diseases
#11
REVIEW
Taro Tsujimura, Mana Idei, Masahiro Yoshikawa, Osamu Takase, Keiichi Hishikawa
The gene encoding bone morphogenetic protein-7 (Bmp7) is expressed in the developing kidney in embryos and also in the mature organ in adults. During kidney development, expression of Bmp7 is essential to determine the final number of nephrons in and proper size of the organ. The secreted BMP7 acts on the nephron progenitor cells to exert its dual functions: To maintain and expand the progenitor population and to provide them with competence to respond to differentiation cues, each relying on distinct signaling pathways...
September 26, 2016: World Journal of Stem Cells
https://www.readbyqxmd.com/read/27660075/hnf-4alpha-negatively-regulates-hepcidin-expression-through-bmpr1a-in-hepg2-cells
#12
Wencai Shi, Heyang Wang, Xuan Zheng, Xin Jiang, Zheng Xu, Hui Shen, Min Li
Hepcidin synthesis is reported to be inadequate according to the body iron store in patients with non-alcoholic fatty liver disease (NAFLD) undergoing hepatic iron overload (HIO). However, the underlying mechanisms remain unclear. We hypothesize that hepatocyte nuclear factor-4α (HNF-4α) may negatively regulate hepcidin expression and contribute to hepcidin deficiency in NAFLD patients. The effect of HNF-4α on hepcidin expression was observed by transfecting specific HNF-4α small interfering RNA (siRNA) or plasmids into HepG2 cells...
September 23, 2016: Biological Trace Element Research
https://www.readbyqxmd.com/read/27648196/insight-on-bone-morphogenetic-protein-7-in-ankylosing-spondylitis-and-its-association-with-disease-activity-and-radiographic-damage
#13
Adel Mahmoud, Dalia Fayez, Mervat Mammdouh Abou Gabal, Sherin Mohamed Hosny Hamza, Takwa Badr
INTRODUCTION: Fusion of joints as well as intervertebral spaces by the formation of bony spurs appearing as syndesmophytes and osteophytes are the hallmark of spondyloarthropathies which accounts for disability. The aim of this study was to assess the serum level of bone morphogenetic protein (BMP)-7 in ankylosing spondylitis and its relationship with disease activity and the radiographic damage. METHODS: This longitudinal case control study was conducted in Ain Shams University Hospitals (Egypt)...
July 2016: Electronic Physician
https://www.readbyqxmd.com/read/27633082/effect-of-bmps-and-wnt3a-co-expression-on-the-osteogenetic-capacity-of-osteoblasts
#14
Wendong Ruan, Yuan Xue, Yaqi Zong, Chao Sun
In the present study, third‑generation autologous‑inactivated bone morphogenic protein 2 (BMP2), BMP4, BMP6, BMP7, BMP9 and Wnt3a lentiviral vectors were constructed and integrated into the genome of MC3T3‑E1 murine mesenchymal stem cells (MMSCs) to produce osteoinductive factor gene‑modified MMSCs. The transfection efficiency of each osteoinductive factor was then determined by detecting the expression levels of runt related transcription factor 2 (Runx2) mRNA. The cotransfection with combinations of two lentiviruses was performed, and the expression levels of bone γ‑carboxyglutamate protein and alkaline phosphatase in the MC3T3‑E1 cell culture supernatant were detected...
September 12, 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/27617228/treatment-with-recombinant-human-bone-morphogenetic-protein-7-leads-to-a-transient-induction-of-neutralizing-autoantibodies-in-a-subset-of-patients
#15
Andrea Schuette, Arash Moghaddam, Petra Seemann, Georg N Duda, Gerhard Schmidmaier, Lutz Schomburg
BACKGROUND: Recombinant human bone morphogenetic protein 7 (rhBMP7) is applied for treatment of bone fractures, especially tibial non-unions. Its application may induce autoantibodies (aAB) affecting the targeted and endogenous signaling pathways and in turn negatively impact treatment efficacy. METHODS: Novel and sensitive assays for the quantification of BMP7-aAB and BMP2-aAB were established and used to analyze serum samples from healthy controls (n = 100 men, n = 100 women) and patients with long bone fracture (n = 265) treated or not with rhBMP7...
December 2016: BBA Clinical
https://www.readbyqxmd.com/read/27582519/bmp7-based-functionalized-self-assembling-peptides-protect-nucleus-pulposus-derived-stem-cells-from-apoptosis-in-vitro
#16
Xiao-Chuan Li, Yao-Hong Wu, Xue-Dong Bai, Wei Ji, Zi-Ming Guo, Chao-Feng Wang, Qing He, Di-Ke Ruan
Tissue engineering has shown great success in the treatment of intervertebral disk degeneration (IVDD) in the past decade. However, the adverse and harsh microenvironment associated in the intervertebral disks remains a great obstacle for the survival of transplanted cells. Although increasing numbers of new materials have been created or modified to overcome this hurdle, a new effective strategy of biological therapy is still required. In this study, bone morphogenic protein 7 (BMP7)-based functionalized self-assembling peptides were developed by conjugating a bioactive motif from BMP-7 (RKPS) onto the C-terminal of the peptide RADARADARADARADA (RADA16-I) at a ratio of 1:1 to form a new RADARKPS peptide...
October 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27581786/drug-resistant-cxcr4-positive-cells-have-the-molecular-characteristics-of-emt-in-nsclc
#17
Hanlu Yin, Yi Wang, Wenping Chen, Shanliang Zhong, Zhian Liu, Jianhua Zhao
High expression of Chemokine receptor 4 (CXCR4) is important in tumor invasion, metastasis, drug-resistance and maintenance of stemness in non-small cell lung cancer (NSCLC). We therefore studied the molecular characteristics of drug-resistant CXCR4-positive cells on epithelial-mesenchymal transition (EMT) for the future identification of the tumor cells with the properties of both EMT and stemness. EMT RT(2) Profier PCR Array was performed to determine the expression levels of mRNA genes in A549 with TGF-β1 induced EMT (A549/TGF-β1) and gefitinib-resistant CXCR4-positive cells (A549/GR)...
December 5, 2016: Gene
https://www.readbyqxmd.com/read/27525902/candidate-genes-for-the-development-of-hair-follicles-in-hu-sheep
#18
X Y Lv, R Ni, W Sun, R Su, H H Musa, J F Yin, Q Z Wang, W Gao, L Chen
The aim of this study was to detect candidate genes for the development of hair follicles in the Hu sheep breed. Seven genes have been detected in large, medium, and small wave follicles of Hu sheep using gene chip technology. The histological features of the follicles of newborn Hu-lambs were combined with fluorescence quantitative PCR technology to detect the correlation between the expression of the seven genes and hair follicle development. Among the genes studied, matrix metalloproteinase 2 (MMP2), bone morphogenetic protein-7 (BMP7), and sideroflexin 1 (SFXN1) showed a significantly different pattern of expression in large, medium, and small wave follicles (P < 0...
2016: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/27491681/bmp7-retards-peripheral-myelination-by-activating-p38-mapk-in-schwann-cells
#19
Xiaoyu Liu, Yahong Zhao, Su Peng, Shuqiang Zhang, Meihong Wang, Yeyue Chen, Shan Zhang, Yumin Yang, Cheng Sun
Schwann cell (SC) myelination is pivotal for the proper physiological functioning of the nervous system, but the underlying molecular mechanism remains less well understood. Here, we showed that the expression of bone morphogenetic protein 7 (BMP7) inversely correlates with myelin gene expression during peripheral myelination, which suggests that BMP7 is likely a negative regulator for myelin gene expression. Our experiments further showed that the application of BMP7 attenuates the cAMP induced myelin gene expression in SCs...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27432514/fgf-and-bmp-derived-from-dorsal-root-ganglia-regulate-blastema-induction-in-limb-regeneration-in-ambystoma-mexicanum
#20
Akira Satoh, Aki Makanae, Yurie Nishimoto, Kazumasa Mitogawa
Urodele amphibians have a remarkable organ regeneration ability that is regulated by neural inputs. The identification of these neural inputs has been a challenge. Recently, Fibroblast growth factor (Fgf) and Bone morphogenic protein (Bmp) were shown to substitute for nerve functions in limb and tail regeneration in urodele amphibians. However, direct evidence of Fgf and Bmp being secreted from nerve endings and regulating regeneration has not yet been shown. Thus, it remained uncertain whether they were the nerve factors responsible for successful limb regeneration...
September 1, 2016: Developmental Biology
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