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https://www.readbyqxmd.com/read/29150636/a-genetic-system-for-targeted-mutations-to-disrupt-and-restore-genes-in-the-obligate-bacterium-ehrlichia-chaffeensis
#1
Ying Wang, Lanjing Wei, Huitao Liu, Chuanmin Cheng, Roman R Ganta
Obligate intracellular bacteria (obligates) belonging to Rickettsiales and Chlamydiales cause diseases in hundreds of millions of people worldwide and in many animal species. Lack of an efficient system for targeted mutagenesis in obligates remains a major impediment in understanding microbial pathogenesis. Challenges in creating targeted mutations may be attributed to essential nature of majority of the genes and intracellular replication dependence. Despite success in generating random mutations, a method that works well in creating mutations in specific genes of interest followed by complementation remains problematic for obligates and is a highly sought-after goal...
November 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29150323/screening-of-patient-blood-samples-for-babesiosis-using-enzymatic-assays
#2
Shekerah Primus, Lavoisier Akoolo, Samantha Schlachter, Nikhat Parveen
Human babesiosis is an emerging tick-borne disease in the United States and Europe. Transmitted by Ixodes ticks, the causative agent Babesia microti is an intraerythrocytic parasite that causes mild to deadly disease. Transmission of B. microti can also occur by transfusion of infected blood and blood products resulting in transfusion-transmitted babesiosis (TTB), which carries a high risk of fatality. To effectively manage this rise in B. microti infections, better screening tools are needed, which require minimal manipulation of the samples before testing...
November 12, 2017: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29149298/vector-borne-diseases-and-climate-change-a-european-perspective
#3
Jan C Semenza, Jonathan E Suk
Climate change has already impacted the transmission of a wide-range of vector-borne diseases in Europe, and it will continue to do so in the coming decades. Climate change has been implicated in the observed shift of ticks to elevated altitudes and latitudes, notably including the Ixodes ricinus tick species which is a vector for Lyme borreliosis and tick-borne encephalitis. Climate change is also thought to have been a factor in the expansion of other important disease vectors in Europe: Aedes albopictus (the Asian tiger mosquito), which transmits diseases such as Zika, dengue, and chikungunya, and Phlebotomus sandfly species, which transmits diseases including Leishmaniasis...
November 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29149281/seroreactivity-to-the-c6-peptide-in-borrelia-miyamotoi-infections-occurring-in-the-northeastern-united-states
#4
Phillip J Molloy, Karen E Weeks, Brittany Todd, Gary P Wormser
Background: There are no Food and Drug Administration (FDA) approved diagnostic tests for Borrelia miyamotoi infection, an emerging tick-borne illness in the United States. The purpose of this study was to evaluate whether the FDA-approved C6 peptide ELISA currently used to diagnose Lyme disease may potentially serve as a diagnostic test for B. miyamotoi infections. Methods: Serum specimens from 30 patients from the Northeastern United States with B. miyamotoi infection established by a polymerase chain reaction assay of a blood specimen were tested using the C6 ELISA...
November 15, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29148377/tick-borne-encephalitis-in-sheep-romania
#5
Jiri Salat, Andrei D Mihalca, Marian Mihaiu, David Modrý, Daniel Ruzek
Little is known about the occurrence of tick-borne encephalitis in Romania. Sheep are an infection source for humans and are useful sentinels for risk analysis. We demonstrate high antibody prevalence (15.02%) among sheep used as sentinels for this disease in 80% of the tested localities in 5 counties of northwestern Romania.
December 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29147886/peromyscus-leucopus-mouse-brain-transcriptome-response-to-powassan-virus-infection
#6
Luwanika Mlera, Kimberly Meade-White, Eric Dahlstrom, Rachel Baur, Kishore Kanakabandi, Kimmo Virtaneva, Stephen F Porcella, Marshall E Bloom
Powassan virus (POWV) is a tick-borne Flavivirus responsible for life-threatening encephalitis in North America and some regions of Russia. The ticks that have been reported to transmit the virus belong to the Ixodes species, and they feed on small-to-medium-sized mammals, such as Peromyscus leucopus mice, skunks, and woodchucks. We previously developed a P. leucopus mouse model of POWV infection, and the model is characterized by a lack of clinical signs of disease following intraperitoneal or intracranial inoculation...
November 16, 2017: Journal of Neurovirology
https://www.readbyqxmd.com/read/29137670/modelling-and-mapping-tick-dynamics-using-volunteered-observations
#7
Irene Garcia-Martí, Raúl Zurita-Milla, Arnold J H van Vliet, Willem Takken
BACKGROUND: Tick populations and tick-borne infections have steadily increased since the mid-1990s posing an ever-increasing risk to public health. Yet, modelling tick dynamics remains challenging because of the lack of data and knowledge on this complex phenomenon. Here we present an approach to model and map tick dynamics using volunteered data. This approach is illustrated with 9 years of data collected by a group of trained volunteers who sampled active questing ticks (AQT) on a monthly basis and for 15 locations in the Netherlands...
November 14, 2017: International Journal of Health Geographics
https://www.readbyqxmd.com/read/29133662/a-tiny-tick-can-cause-a-big-health-problem
#8
Manuel John, M Raman, Keith Ryan
Ticks are tiny crawling bugs in the spider family that feed by sucking blood from animals. They are second only to mosquitoes as vectors of human disease, both infectious and toxic. Infected ticks spread over a hundred diseases, some of which are fatal if undetected. They spread the spirochete (which multiplies in the insect's gut) with a subsequent bite to the next host. We describe the only reported cases of peri ocular tick bite from India that presented to us within a span of 3 days and its management. Due suspicion and magnification of the lesions revealed the ticks which otherwise masqueraded as small skin tags/moles on gross examination...
November 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29132437/hap2-a-novel-gene-in-babesia-bigemina-is-expressed-in-tick-stages-and-specific-antibodies-block-zygote-formation
#9
Minerva Camacho-Nuez, Diego Josimar Hernández-Silva, Elizabeth Jacqueline Castañeda-Ortiz, María Elena Paredes-Martínez, Marisol Karina Rocha-Martínez, María Elizbeth Alvarez-Sánchez, Ricardo Francisco Mercado-Curiel, Gabriela Aguilar-Tipacamu, Juan Mosqueda
BACKGROUND: Bovine babesiosis is a tick-borne disease caused by the protozoan parasites of the genus Babesia. In their host vector, Babesia spp. undergo sexual reproduction. Therefore, the development of sexual stages and the subsequent formation of the zygote are essential for the parasite to invade the intestinal cells of the vector tick and continue its life-cycle. HAP2/GCS1 is a protein identified in plants, protozoan parasites and other organisms that has an important role during membrane fusion in fertilization processes...
November 13, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29127993/molecular-prevalence-risk-factors-assessment-and-haemato-biochemical-alterations-in-hepatozoonosis-in-dogs-from-punjab-india
#10
Kaushlendra Singh, Harkirat Singh, Nirbhay Kumar Singh, Neeraj Kashyap, Naresh Kumar Sood, Shitanshu Shekar Rath
Hepatozoonosis caused by Hepatozoon canis is an important tick-borne disease of dogs in tropical and sub-tropical regions throughout the world. In the present study evaluation of blood samples collected from 225 dogs presented at Small Animal Clinics, GADVASU, Ludhiana, Punjab (India) was done for the presence of H. canis by PCR based assay targeting a portion of 18S rRNA gene. Of the total samples subjected to PCR, an amplicon of 666bp was detected in 13.78% samples whereas, routine blood smear examination revealed gamonts in 5...
December 2017: Comparative Immunology, Microbiology and Infectious Diseases
https://www.readbyqxmd.com/read/29127407/crystal-structure-of-borrelia-turicatae-protein-bta121-a-differentially-regulated-%C3%A2-gene-in-the-tick-mammalian-transmission-cycle-of-relapsing-fever-spirochetes
#11
Zhipu Luo, Alan J Kelleher, Rabih Darwiche, Elissa M Hudspeth, Oluwatosin K Shittu, Aparna Krishnavajhala, Roger Schneiter, Job E Lopez, Oluwatoyin A Asojo
Tick-borne relapsing fever (RF) borreliosis is a neglected disease that is often misdiagnosed. RF species circulating in the United States include Borrelia turicatae, which is transmitted by argasid ticks. Environmental adaptation by RF Borrelia is poorly understood, however our previous studies indicated differential regulation of B. turicatae genes localized on the 150 kb linear megaplasmid during the tick-mammalian transmission cycle, including bta121. This gene is up-regulated by B. turicatae in the tick versus the mammal, and the encoded protein (BTA121) is predicted to be surface localized...
November 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29125245/first-records-of-ixodes-lividus-from-sand-martin-riparia-riparia-nests-in-lithuania
#12
Vesta Matulaitytė, Jana Radzijevskaja, Algimantas Paulauskas
Ixodes lividus (Koch, 1844) ticks are specific parasites of the sand martin Riparia riparia (L.). The distribution range of I. lividus covers Europe (being absent from the Mediterranean area) and Asia. However, until now there have been no reports on the presence of this tick in Lithuania. A total of 47 nests were collected in three different colonies of sand martins in the central part of Lithuania and 46.8% of them were infested with ticks. In total, 2,770 ticks were found and identified as I. lividus based on morphological characteristics...
December 2017: Journal of Vector Ecology: Journal of the Society for Vector Ecology
https://www.readbyqxmd.com/read/29121976/prevalence-of-tick-borne-pathogens-in-questing-ixodes-ricinus-ticks-in-urban-and-suburban-areas-of-switzerland
#13
Corinne P Oechslin, Daniel Heutschi, Nicole Lenz, Werner Tischhauser, Olivier Péter, Olivier Rais, Christian M Beuret, Stephen L Leib, Sergei Bankoul, Rahel Ackermann-Gäumann
BACKGROUND: Throughout Europe, Ixodes ricinus transmits numerous pathogens. Its widespread distribution is not limited to rural but also includes urbanized areas. To date, comprehensive data on pathogen carrier rates of I. ricinus ticks in urban areas of Switzerland is lacking. RESULTS: Ixodes ricinus ticks sampled at 18 (sub-) urban collection sites throughout Switzerland showed carrier rates of 0% for tick-borne encephalitis virus, 18.0% for Borrelia burgdorferi (sensu lato), 2...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29120859/tick-borne-infections-and-co-infections-in-patients-with-non-specific-symptoms-in-poland-tick-borne-infections-and-co-infections
#14
Justyna Dunaj, Anna Moniuszko-Malinowska, Izabela Swiecicka, Martin Andersson, Piotr Czupryna, Krzysztof Rutkowski, Grzegorz Zambrowski, Joanna Zajkowska, Sambor Grygorczuk, Maciej Kondrusik, Renata Świerzbińska, Sławomir Pancewicz
AIM: The aim of the study was the evaluation of the frequency of infections and co-infections among patients hospitalized because of non-specific symptoms after a tick bite. MATERIALS AND METHODS: Whole blood, serum and cerebrospinal fluid samples from 118 patients hospitalised for non-specific symptoms up to 8 weeks after tick bite from 2010 to 2013 were examined for tick-borne infections. ELISA, Western blot and/or molecular biology (PCR; fla gene; 16S rRNA; sequencing) and thin blood smears (MDD) were used...
November 7, 2017: Advances in Medical Sciences
https://www.readbyqxmd.com/read/29120298/ictv-virus-taxonomy-profile-nyamiviridae
#15
Ralf G Dietzgen, Elodie Ghedin, Dàohóng Jiāng, Jens H Kuhn, Timothy Song, Nikos Vasilakis, David Wang, Ictv Report Consortium
The Nyamiviridae is a family of viruses with unsegmented, negative-sense RNA genomes of 11.3-12.2 kb that produce enveloped, spherical virions. Viruses of the genus Nyavirus are tick-borne and some also infect birds. Other nyamiviruses infecting parasitoid wasps and plant parasitic nematodes have been classified into the genera Peropuvirus and Socyvirus, respectively. This is a summary of the current International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of Nyamiviridae, which is available at www...
November 9, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29119703/rickettsia-parkeri-in-free-ranging-wild-canids-from-brazilian-pampa
#16
B Dall'Agnol, U A Souza, B Weck, T C Trigo, M M A Jardim, F B Costa, M B Labruna, F B Peters, M O Favarini, F D Mazim, C A S Ferreira, J Reck
Spotted fevers are tick-borne diseases associated with various Rickettsia species. Rickettsia parkeri sensu stricto (s.s.) is the agent of an emerging eschar-associated rickettsiosis in humans from the USA and South American Pampa. Considering that R. parkeri s.s. is restricted to Americas and the potential role of dogs in the epidemiology of the disease, it is thus reasonable to hypothesize that wild canids could be involved in the enzootic cycle of this rickettsiosis. The aim of this work was to investigate the potential role of the wild canids from Pampa, Cerdocyon thous (crab-eating fox) and Lycalopex gymnocercus (Pampas fox), in the ecology of R...
November 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29119682/molecular-identification-of-tick-borne-pathogens-infecting-cattle-in-mymensingh-district-of-bangladesh-reveals-emerging-species-of-anaplasma-and-babesia
#17
B C Roy, J Krücken, J S Ahmed, S Majumder, M P Baumann, P-H Clausen, A M Nijhof
Tick-borne diseases are considered a major hindrance to the health and productive performance of cattle in Bangladesh. To elucidate the epidemiology of tick-borne pathogens (TBPs) in local cattle, a cross-sectional study was performed in the 12 subdistricts (Upazilas) of Mymensingh district in Bangladesh. Blood samples and ticks were collected from 384 clinically healthy cattle kept by 135 farmers from 96 randomly selected villages. DNA extracted from the blood samples was subsequently screened by polymerase chain reaction (PCR) and a Reverse Line Blot (RLB) hybridization assay using an in-house prepared chemiluminescence solution for the presence of Anaplasma, Ehrlichia, Rickettsia, Babesia and Theileria spp...
November 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29116038/borrelia-burgdorferi-glycosaminoglycan-binding-proteins-a-potential-target-for-new-therapeutics-against-lyme-disease
#18
Yi-Pin Lin, Lingyun Li, Fuming Zhang, Robert J Linhardt
The spirochete bacterium Borrelia burgdorferisensu lato is the causative agent of Lyme disease, the most common vector-borne disease in Europe and the United States. The spirochetes can be transmitted to humans via ticks, and then spread to different tissues, leading to arthritis, carditis and neuroborreliosis. Although antibiotics have commonly been used to treat infected individuals, some treated patients do not respond to antibiotics and experience persistent, long-term arthritis. Thus, there is a need to investigate alternative therapeutics against Lyme disease...
November 8, 2017: Microbiology
https://www.readbyqxmd.com/read/29110020/granuloma-annulare-and-morphea-correlation-with-borrelia-burgdorferi-infections-and-chlamydia-related-bacteria
#19
Lauri Tolkki, Kati Hokynar, Seppo Meri, Jaana Panelius, Mirja Puolakkainen, Annamari Ranki
A retrospective study of 109 skin biopsies with granuloma annulare (GA) or morphea histology from patients with suspected tick bite was performed. Biopsies were tested for cutaneous Borrelia burgdorferi DNA using PCR. The same biopsies were analysed for tick-borne novel agents, Chlamydia-related bacteria (members of the Chlamydiales order), using a PCR-based method. Borrelia DNA was detected in 7/73 (9.6%) biopsies with GA and in 1/36 (2.8 %) biopsies with morphea, while Chlamydiales DNA was found in 53/73 (72...
November 7, 2017: Acta Dermato-venereologica
https://www.readbyqxmd.com/read/29107656/molecular-prevalence-of-spotted-fever-group-rickettsiae-in-ticks-from-qinghai-province-northwestern-china
#20
Rong Han, Jifei Yang, Qingli Niu, Zhijie Liu, Ze Chen, Wei Kan, Guangwei Hu, Guangyuan Liu, Jianxun Luo, Hong Yin
Tick-borne rickettsioses is one of the oldest known vector-borne diseases and has been viewed as emerging or re-emerging disease in China. The causative agents have been increasingly recognized and exhibited a high degree of genetic diversity and widespread distribution. This study was conducted to determine the prevalence of spotted fever group rickettsiae in ticks from Qinghai Province, northwestern China. In total, 860 questing adult ticks representing six species were collected. The SFG rickettsiae were detected in Haemaphysalis qinghaiensis (19...
October 28, 2017: Infection, Genetics and Evolution
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