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https://www.readbyqxmd.com/read/28454480/immobilization-of-ph-sensitive-cdte-quantum-dots-in-a-poly-acrylate-hydrogel-for-microfluidic-applications
#1
M Franke, S Leubner, A Dubavik, A George, T Savchenko, C Pini, P Frank, D Melnikau, Y Rakovich, N Gaponik, A Eychmüller, A Richter
Microfluidic devices present the basis of modern life sciences and chemical information processing. To control the flow and to allow optical readout, a reliable sensor material that can be easily utilized for microfluidic systems is in demand. Here, we present a new optical readout system for pH sensing based on pH sensitive, photoluminescent glutathione capped cadmium telluride quantum dots that are covalently immobilized in a poly(acrylate) hydrogel. For an applicable pH sensing the generated hybrid material is integrated in a microfluidic sensor chip setup...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28448040/determination-of-high-affinity-antibody-antigen-binding-kinetics-using-four-biosensor-platforms
#2
Danlin Yang, Ajit Singh, Helen Wu, Rachel Kroe-Barrett
Label-free optical biosensors are powerful tools in drug discovery for the characterization of biomolecular interactions. In this study, we describe the use of four routinely used biosensor platforms in our laboratory to evaluate the binding affinity and kinetics of ten high-affinity monoclonal antibodies (mAbs) against human proprotein convertase subtilisin kexin type 9 (PCSK9). While both Biacore T100 and ProteOn XPR36 are derived from the well-established Surface Plasmon Resonance (SPR) technology, the former has four flow cells connected by serial flow configuration, whereas the latter presents 36 reaction spots in parallel through an improvised 6 x 6 crisscross microfluidic channel configuration...
April 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28442789/elastic-turbulence-in-entangled-semi-dilute-dna-solutions-measured-with-optical-coherence-tomography-velocimetry
#3
A V Malm, T A Waigh
The flow instabilities of solutions of high molecular weight DNA in the entangled semi-dilute concentration regime were investigated using optical coherence tomography velocimetry, a technique that provides high spatial (probe volumes of 3.4 pL) and temporal resolution (sub μs) information on the flow behaviour of complex fluids in a rheometer. The velocity profiles of the opaque DNA solutions (high and low salt) were measured as a function of the distance across the gap of a parallel plate rheometer, and their evolution over time was measured...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28441929/endothelial-progenitor-cells-and-plaque-burden-in-stented-coronary-artery-segments-an-optical-coherence-tomography-study-six-months-after-elective-pci
#4
Sylvia Otto, Kristina Nitsche, Christian Jung, Aleh Kryvanos, Andrey Zhylka, Kerstin Heitkamp, Juan-Luis Gutiérrez-Chico, Björn Goebel, P Christian Schulze, Hans R Figulla, Tudor C Poerner
BACKGROUND: Endothelial progenitor cells (EPC) are involved in neovascularization and endothelial integrity. They might be protective in atherosclerosis. Optical coherence tomography (OCT) is a precise intracoronary imaging modality that allows assessment of subintimal plaque development. We evaluated the influence of EPC on coronary plaque burden in stable disease and implemented a novel computational plaque analysis algorithm using OCT. METHODS: Forty-three patients (69...
April 26, 2017: BMC Cardiovascular Disorders
https://www.readbyqxmd.com/read/28440273/active-matter-logic-for-autonomous-microfluidics
#5
Francis G Woodhouse, Jörn Dunkel
Chemically or optically powered active matter plays an increasingly important role in materials design, but its computational potential has yet to be explored systematically. The competition between energy consumption and dissipation imposes stringent physical constraints on the information transport in active flow networks, facilitating global optimization strategies that are not well understood. Here, we combine insights from recent microbial experiments with concepts from lattice-field theory and non-equilibrium statistical mechanics to introduce a generic theoretical framework for active matter logic...
April 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28440049/analytical-based-methodologies-for-examining-the-in-vitro-absorption-distribution-metabolism-and-elimination-adme-of-silver-nanoparticles
#6
Sesha L A Paluri, John D Ryan, Nhi H Lam, Dhriti Nepal, Ioana E Sizemore
The clinical applications of silver nanoparticles (AgNPs) remain limited due to the lack of well-established methodologies for studying their nanokinetics. Hereby, the primary goal is to adapt a suite of analytical-based methodologies for examining the in vitro absorption, distribution, metabolism, and elimination of AgNPs. Vero 76 and HEK 293 cells are exposed to ≈10-nm spherical AgNPs(+) and AgNPs(-) at relevant concentrations (0-300 µg mL(-1) ) and times (4-48 h). Absorption: Inductively coupled plasma optical emission spectroscopy (ICP-OES) demonstrates that the two AgNP formulations are not bioequivalent...
April 25, 2017: Small
https://www.readbyqxmd.com/read/28439656/-management-of-paraophthalmic-aneurysms-review-of-endovascular-treatment-strategies
#7
P Bhogal, M Aguilar Pérez, G Sauder, H Bäzner, O Ganslandt, H Henkes
BACKGROUND: Aneurysms of the cerebral vessels are relatively common and aneurysms of the ophthalmic segment of the internal carotid artery (ICA) represent approximately 10% of intradural aneurysms. ANATOMICAL ASPECTS: The ophthalmic segment of the ICA extends from the origin of the ophthalmic artery to the origin of the posterior communicating artery. Aneurysms can originate from the ophthalmic artery itself or directly from the ICA at a distance from the ophthalmic artery...
April 24, 2017: Der Ophthalmologe: Zeitschrift der Deutschen Ophthalmologischen Gesellschaft
https://www.readbyqxmd.com/read/28438012/smartphone-based-dual-modality-imaging-system-for-quantitative-detection-of-color-or-fluorescent-lateral-flow-immunochromatographic-strips
#8
Yafei Hou, Kan Wang, Kun Xiao, Weijian Qin, Wenting Lu, Wei Tao, Daxiang Cui
Nowadays, lateral flow immunochromatographic assays are increasingly popular as a diagnostic tool for point-of-care (POC) test based on their simplicity, specificity, and sensitivity. Hence, quantitative detection and pluralistic popular application are urgently needed in medical examination. In this study, a smartphone-based dual-modality imaging system was developed for quantitative detection of color or fluorescent lateral flow test strips, which can be operated anywhere at any time. In this system, the white and ultra-violet (UV) light of optical device was designed, which was tunable with different strips, and the Sobel operator algorithm was used in the software, which could enhance the identification ability to recognize the test area from the background boundary information...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28437968/vortex-energy-flows-generated-by-core-shell-nanospheres
#9
Shih-Wen Chen, Jia-Han Li
Investigated in this paper is the interaction of light and the nanospheres composed by a dielectric core with a gold-shell cladding that causes the optical vortices inside the core and the whirlpools around the shell. Different radius ratios, dimensions and the dielectric functions of nanospheres were studied using the finite-difference time-domain method. It was found that optical vortices were most likely to occur in the regions of increased absorption cross section and reduced scattering cross section. Two optical vortices of the opposite polarity, each centered in one of the particles of a dimer are created by a nanoshell dimer...
April 17, 2017: Optics Express
https://www.readbyqxmd.com/read/28437441/fluorescence-imaging-topography-scanning-system-for-intraoperative-multimodal-imaging
#10
Tri T Quang, Hye-Yeong Kim, Forrest Sheng Bao, Francis A Papay, W Barry Edwards, Yang Liu
Fluorescence imaging is a powerful technique with diverse applications in intraoperative settings. Visualization of three dimensional (3D) structures and depth assessment of lesions, however, are oftentimes limited in planar fluorescence imaging systems. In this study, a novel Fluorescence Imaging Topography Scanning (FITS) system has been developed, which offers color reflectance imaging, fluorescence imaging and surface topography scanning capabilities. The system is compact and portable, and thus suitable for deployment in the operating room without disturbing the surgical flow...
2017: PloS One
https://www.readbyqxmd.com/read/28436865/video-anomaly-detection-with-compact-feature-sets-for-online-performance
#11
Roberto Leyva, Victor Sanchez, Chang-Tsun Li
Over the past decade, video anomaly detection has been explored with remarkable results. However, research on methodologies suitable for online performance is still very limited. In this paper, we present an online framework for video anomaly detection. The key aspect of our framework is a compact set of highly descriptive features, which is extracted from a novel cell structure that helps to define support regions in a coarse-to-fine fashion. Based on the scene's activity, only a limited number of support regions are processed, thus limiting the size of the feature set...
April 18, 2017: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/28436842/a-hybrid-image-registration-and-matching-framework-for-real-time-motion-tracking-in-mri-guided-radiotherapy
#12
Matteo Seregni, Chiara Paganelli, Paul Summers, Massimo Bellomi, Guido Baroni, Marco Riboldi
OBJECTIVE: MRI-guided radiotherapy (MRIgRT) is an emerging treatment technique where anatomical and pathological structures are imaged through integrated MR-radiotherapy units. This work aims (i) at assessing the accuracy of optical-flow based motion tracking in liver cine-MRI sequences and (ii) at testing a MRIgRT workflow combining similarity-based image matching with image registration. METHODS: After an initialization stage, a set of template images is collected and registered to the first frame of the cine-MRI sequence...
April 24, 2017: IEEE Transactions on Bio-medical Engineering
https://www.readbyqxmd.com/read/28433988/comparison-of-choriocapillaris-flow-measurements-between-two-optical-coherence-tomography-angiography-devices
#13
Jost L Lauermann, Peter Heiduschka, Pieter Nelis, Maximilian Treder, Maged Alnawaiseh, Christoph R Clemens, Nicole Eter, Florian Alten
PURPOSE: To evaluate choriocapillaris (CC) perfusion in healthy subjects using 2 different optical coherence tomography angiography (OCT-A) devices. PROCEDURES: Macular OCT-A imaging (36 eyes of 36 subjects) was performed using Optovue AngioVue and Zeiss AngioPlex devices. CC decorrelation signal index was assessed, and CC data were analyzed regarding intra-device variability, inter-device correlation, age, signal strength, and fields of view. RESULTS: The intra-device variability of CC measurements in the 3 × 3 mm2 field was 5...
April 22, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28432955/the-calorimetric-properties-of-liposomes-determine-the-morphology-of-dried-droplets
#14
Jorge González-Gutiérrez, Rosendo Pérez-Isidoro, M I Pérez-Camacho, J C Ruiz-Suárez
The evaporation of liquid droplets deposited on a substrate is a very complex phenomenon. Driven by capillary and Marangoni flows, particle-particle and particle-substrate interactions, the deposits they leave are vestiges of such complexity. We study the formation of patterns during the evaporation of liposome suspension droplets deposited on a hydrophobic substrate at different temperatures. We observed that as we change the temperature of the substrate, a morphological phase transition occurs at a given temperature Tm...
April 19, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28430305/turbulence-measurements-in-an-axial-rotary-blood-pump-with-laser-doppler-velocimetry
#15
Chan Y Schüle, Klaus Affeld, Max Kossatz, Christian O Paschereit, Ulrich Kertzscher
BACKGROUND: The implantation of rotary blood pumps as ventricular assist devices (VADs) has become a viable therapy for quite a number of patients with end-stage heart failure. However, these rotary blood pumps cause adverse events that are related to blood trauma. It is currently believed that turbulence in the pump flow plays a significant role. But turbulence has not been measured to date because there is no optical access to the flow space in rotary blood pumps because of their opaque casings...
April 18, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28430261/wideband-spectrum-analysis-of-ultra-high-frequency-radio-wave-signals-due-to-advanced-one-phonon-non-collinear-anomalous-light-scattering
#16
Alexandre S Shcherbakov, Adan Omar Arellanes
We present a principally new acousto-optical cell providing an advanced wideband spectrum analysis of ultra-high frequency radio-wave signals. For the first time, we apply a recently developed approach with the tilt angle to a one-phonon non-collinear anomalous light scattering. In contrast to earlier cases, now one can exploit a regime with the fixed optical wavelength for processing a great number of acoustic frequencies simultaneously in the linear regime. The chosen rutile-crystal combines a moderate acoustic velocity with low acoustic attenuation and allows us wide-band data processing within GHz-frequency acoustic waves...
April 20, 2017: Applied Optics
https://www.readbyqxmd.com/read/28429817/investigation-of-bacterial-viability-from-incubated-saliva-by-application-of-flow-cytometry-and-hyphenated-separation-techniques
#17
Bogusław Buszewski, Maciej Milanowski, Tomasz Ligor, Paweł Pomastowski
The aim of the study was determination of bacterial viability in saliva samples and finding a correlation between microbiological and volatile profiles of saliva depending on incubation time. Bacteria colonizing healthy oral cavities were also identified. Twelve healthy adults donated unstimulated saliva samples. Flow cytometry, optical density measurements and colony-forming unit (CFU) counting method were employed for analyses of native and inoculated saliva after 0, 1, 2, 24 and 48 h of incubation. Volatile profiles were acquired using headspace-solid phase microextraction-gas chromatography/mass spectrometry (HS-SPME-GC/MS)...
April 21, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28429124/how-bumblebees-use-lateral-and-ventral-optic-flow-cues-for-position-control-in-environments-of-different-proximity
#18
Nellie Linander, Emily Baird, Marie Dacke
Flying insects frequently navigate through environments of different complexity. In this study, buff-tailed bumblebees (Bombus terrestris L.) were trained to fly along tunnels of different widths, from 60 to 240 cm. In tunnel widths of 60 and 120 cm, bumblebees control their lateral position by balancing the magnitude of translational optic flow experienced in the lateral visual field of each eye. In wider tunnels, bumblebees use translational optic flow cues in the ventral visual field to control their lateral position and to steer along straight tracks...
April 20, 2017: Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology
https://www.readbyqxmd.com/read/28427411/tendon-motion-tracking-in-an-ultrasound-image-sequence-using-optical-flow-based-block-matching
#19
Bo-I Chuang, Jian-Han Hsu, Li-Chieh Kuo, I-Ming Jou, Fong-Chin Su, Yung-Nien Sun
BACKGROUND: Tendon motion, which is commonly observed using ultrasound imaging, is one of the most important features used in tendinopathy diagnosis. However, speckle noise and out-of-plane issues make the tracking process difficult. Manual tracking is usually time consuming and often yields inconsistent results between users. METHODS: To automatically track tendon motion in ultrasound images, we developed a new method that combines the advantages of optical flow and multi-kernel block matching...
April 20, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28426631/directional-changes-in-tissue-reflectivity-may-influence-flow-detection-on-optical-coherence-tomography-angiography
#20
Rosa Dolz-Marco, K Bailey Freund
PURPOSE: To determine if directional or anatomical changes in tissue reflectivity of Henle fiber layer (HFL) may influence flow detection on optical coherence tomography angiography (OCT-A). METHODS: Cross-sectional analysis of consecutive cases undergoing OCT-A. Directional changes in visualization of HFL were either produced manually by moving the beam entrance within the pupil or observed occurring naturally because of tilting of the OCT line-scan within a myopic staphyloma...
April 19, 2017: Retina
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