keyword
MENU ▼
Read by QxMD icon Read
search

Zebrafish disease modelling

keyword
https://www.readbyqxmd.com/read/29341136/phylogeny-and-distribution-of-protein-kinase-c-variants-in-the-zebrafish
#1
Marion F Haug, Matthias Gesemann, Manuela Berger, Stephan C F Neuhauss
Conventional protein kinases - consisting of α, β, and γ family members - play key roles in numerous signal transduction events. Phylogenetic analysis demonstrated the existence of five prkcs (the genes representing PKCs) in zebrafish, two paralogous forms of prkca and -b and one prkcg variant. mRNA expression analysis showed distinct, mainly nervous system specific expression, for all five prkc genes. For prkca and prkcb paralogs prominent expression can be seen in the telencephalon, in diencephalic regions such as the habenula or the optic tectum, in hypothalamic areas and in distinct cerebellar structures...
January 17, 2018: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/29339520/targeted-knockout-of-a-chemokine-like-gene-increases-anxiety-and-fear-responses
#2
Jung-Hwa Choi, Yun-Mi Jeong, Sujin Kim, Boyoung Lee, Krishan Ariyasiri, Hyun-Taek Kim, Seung-Hyun Jung, Kyu-Seok Hwang, Tae-Ik Choi, Chul O Park, Won-Ki Huh, Matthias Carl, Jill A Rosenfeld, Salmo Raskin, Alan Ma, Jozef Gecz, Hyung-Goo Kim, Jin-Soo Kim, Ho-Chul Shin, Doo-Sang Park, Robert Gerlai, Bradley B Jamieson, Joon S Kim, Karl J Iremonger, Sang H Lee, Hee-Sup Shin, Cheol-Hee Kim
Emotional responses, such as fear and anxiety, are fundamentally important behavioral phenomena with strong fitness components in most animal species. Anxiety-related disorders continue to represent a major unmet medical need in our society, mostly because we still do not fully understand the mechanisms of these diseases. Animal models may speed up discovery of these mechanisms. The zebrafish is a highly promising model organism in this field. Here, we report the identification of a chemokine-like gene family, samdori (sam), and present functional characterization of one of its members, sam2 We show exclusive mRNA expression of sam2 in the CNS, predominantly in the dorsal habenula, telencephalon, and hypothalamus...
January 16, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29335539/brain-activity-patterns-in-high-throughput-electrophysiology-screen-predict-both%C3%A2-drug-efficacies-and-side-effects
#3
Peter M Eimon, Mostafa Ghannad-Rezaie, Gianluca De Rienzo, Amin Allalou, Yuelong Wu, Mu Gao, Ambrish Roy, Jeffrey Skolnick, Mehmet Fatih Yanik
Neurological drugs are often associated with serious side effects, yet drug screens typically focus only on efficacy. We demonstrate a novel paradigm utilizing high-throughput in vivo electrophysiology and brain activity patterns (BAPs). A platform with high sensitivity records local field potentials (LFPs) simultaneously from many zebrafish larvae over extended periods. We show that BAPs from larvae experiencing epileptic seizures or drug-induced side effects have substantially reduced complexity (entropy), similar to reduced LFP complexity observed in Parkinson's disease...
January 15, 2018: Nature Communications
https://www.readbyqxmd.com/read/29322455/construction-and-use-of-staphylococcus-aureus-strains-to-study-within-host-infection-dynamics
#4
Gareth McVicker, Tomasz K Prajsnar, Simon J Foster
The study of the dynamics that occur during the course of a bacterial infection has been attempted using several methods. Here we discuss the construction of a set of antibiotic-resistant, otherwise-isogenic Staphylococcus aureus strains that can be used to observe the progress of systemic disease in a mouse model at various time-points postinfection. The strains can likewise be used to study the progression of infection in other animal infection models, such as the zebrafish embryo. Furthermore, the use of antibiotic resistance tags provides a convenient system with which to investigate the effect of antimicrobial chemotherapy during disease...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29315381/chchd10-mutations-p-r15l-and-p-g66v-cause-motoneuron-disease-by-haploinsufficiency
#5
Sarah J Brockmann, Axel Freischmidt, Patrick Oeckl, Kathrin Müller, Srinivas K Ponna, Anika M Helferich, Christoph Paone, Jörg Reinders, Kerstin Kojer, Michael Orth, Manu Jokela, Mari Auranen, Bjarne Udd, Andreas Hermann, Karin M Danzer, Peter Lichtner, Paul Walther, Albert C Ludolph, Peter M Andersen, Markus Otto, Petri Kursula, Steffen Just, Jochen H Weishaupt
Mutations in the mitochondrially located protein CHCHD10 cause motoneuron disease by an unknown mechanism. In this study, we investigate the mutations p.R15L and p.G66V in comparison to wild-type CHCHD10 and the non-pathogenic variant p.P34S in vitro, in patient cells as well as in the vertebrate in vivo model zebrafish. We demonstrate a reduction of CHCHD10 protein levels in p.R15L and p.G66V mutant patient cells to approximately 50%. Quantitative real-time PCR revealed that expression of CHCHD10 p.R15L, but not of CHCHD10 p...
January 5, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29304309/susceptibility-of-zebrafish-to-vesicular-stomatitis-virus-infection
#6
Jorge Guerra-Varela, Maite Baz-Martínez, Sabela Da Silva-Álvarez, Ana Paula Losada, María Isabel Quiroga, Manuel Collado, Carmen Rivas, Laura Sánchez
The zebrafish, Danio rerio, has become recognized as a valuable model for infectious diseases. Here we evaluated the susceptibility of zebrafish to be infected with the mammalian vesicular stomatitis virus (VSV). Both zebrafish cells and embryos were highly susceptible to VSV infection. Mortalities exceeded 80% in infected embryos and were preceded by the invasion of the central nervous system by VSV. Live imaging of the infection with GFP-VSV as well as virus titration from infected fish confirmed the viral replication...
January 5, 2018: Zebrafish
https://www.readbyqxmd.com/read/29301275/generation-and-characterization-of-antibodies-against-opioid-receptors-from-zebrafish
#7
Juan C Arévalo, Enrique Hernández-Jiménez, Ada Jiménez-González, María Torres-Valle, Roman S Iwasaki, Roger López-Bellido, Cristina Vicente-García, Raquel E Rodríguez
The opioid system is well conserved among species and plays a critical role in pain and addiction systems. The use of zebrafish as an experimental model to study development and genetics is extraordinary and has been proven to be relevant for the study of different diseases. The main drawback to its use for the analysis of different pathologies is the lack of protein tools. Antibodies that work in other models are not suitable for zebrafish due to the low degree of homology that exists among the opioid receptor protein sequences in different species...
January 2, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29295655/-not-tonight-zebrafish-the-effects-of-ruta-graveolens-on-reproduction
#8
Mohammad Navid Forsatkar, Maryam HedayatiRad, Ana Carolina Luchiari
CONTEXT: There is growing interest in the pharmacological evaluation of Rue due to its potential to treat a variety of clinical diseases. The plant seems to present potent endocrine disrupting effects, and its excretion and disposal are not a concern. OBJECTIVE: The effects of Ruta graveolens L. (Rutaceae) ethanol extract (RE) on reproductive behaviour, fertility, and steroid and thyroid hormone levels in zebrafish were investigated. MATERIAL AND METHODS: We exposed subjects to varying concentrations of RE, and one-tenth the LC50 concentration (2...
December 2018: Pharmaceutical Biology
https://www.readbyqxmd.com/read/29286475/microstructured-devices-for-optimized-microinjection-and-imaging-of-zebrafish-larvae
#9
Felix Ellett, Daniel Irimia
Zebrafish have emerged as a powerful model of various human diseases and a useful tool for an increasing range of experimental studies, spanning fundamental developmental biology through to large-scale genetic and chemical screens. However, many experiments, especially those related to infection and xenograft models, rely on microinjection and imaging of embryos and larvae, which are laborious techniques that require skill and expertise. To improve the precision and throughput of current microinjection techniques, we developed a series of microstructured devices to orient and stabilize zebrafish embryos at 2 days post fertilization (dpf) in ventral, dorsal, or lateral orientation prior to the procedure...
December 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29286381/development-of-an-in-vitro-assay-to-quantitate-hematopoietic-stem-and-progenitor-cells-hspcs-in-developing-zebrafish-embryos
#10
A C Berrun, D L Stachura
Hematopoiesis is an essential cellular process in which hematopoietic stem and progenitor cells (HSPCs) differentiate into the multitude of different cell lineages that comprise mature blood. Isolation and identification of these HSPCs is difficult because they are defined ex post facto; they can only be defined after their differentiation into specific cell lineages. Over the past few decades, the zebrafish (Danio rerio) has become a model organism to study hematopoiesis. Zebrafish embryos develop ex utero, and by 48 h post-fertilization (hpf) have generated definitive HSPCs...
November 30, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29283402/real-time-monitoring-and-analysis-of-zebrafish-electrocardiogram-with-anomaly-detection
#11
Michael Lenning, Joseph Fortunato, Tai Le, Isaac Clark, Ang Sherpa, Soyeon Yi, Peter Hofsteen, Geethapriya Thamilarasu, Jingchun Yang, Xiaolei Xu, Huy-Dung Han, Tzung K Hsiai, Hung Cao
Heart disease is the leading cause of mortality in the U.S. with approximately 610,000 people dying every year. Effective therapies for many cardiac diseases are lacking, largely due to an incomplete understanding of their genetic basis and underlying molecular mechanisms. Zebrafish (Danio rerio) are an excellent model system for studying heart disease as they enable a forward genetic approach to tackle this unmet medical need. In recent years, our team has been employing electrocardiogram (ECG) as an efficient tool to study the zebrafish heart along with conventional approaches, such as immunohistochemistry, DNA and protein analyses...
December 28, 2017: Sensors
https://www.readbyqxmd.com/read/29282212/musclemotion-a-versatile-open-software-tool-to-quantify-cardiomyocyte-and-cardiac-muscle-contraction-in-vitro-and-in-vivo
#12
Luca Sala, Berend J van Meer, Leon T Tertoolen, Jeroen Bakkers, Milena Bellin, Richard P Davis, Chris N Denning, Michel A Dieben, Thomas Eschenhagen, Elisa Giacomelli, Catarina Grandela, Arne Hansen, Eduard Holman, Monique R Jongbloed, Sarah M Kamel, Charlotte D Koopman, Quentin Lachaud, Ingra Mannhardt, Mervyn P Mol, Diogo Mosqueira, Valeria V Orlova, Robert Passier, Marcelo C Ribeiro, Umber Saleem, Godfrey Smith, Francis L L Burton, Christine L Mummery
Rationale: There are several methods to measure cardiomyocyte (CM) and muscle contraction but these require customized hardware, expensive apparatus and advanced informatics or can only be used in single experimental models. Consequently, data and techniques have been difficult to reproduce across models and laboratories, analysis is time consuming and only specialist researchers can quantify data. Objective: Here we describe and validate an automated, open source software tool (MUSCLEMOTION) adaptable for use with standard laboratory- and clinical imaging equipment that enables quantitative analysis of normal cardiac contraction, disease phenotypes and pharmacological responses...
December 27, 2017: Circulation Research
https://www.readbyqxmd.com/read/29277889/rhoa-stimulated-intra-capillary-morphology-switch-facilitates-the-arrest-of-individual-circulating-tumor-cells
#13
Xi Huang, Yu Yang, Yuwei Zhao, Cao Dan, Xiaolin Ai, Anqi Zeng, Maling Gou, Lulu Cai, Hanshuo Yang, Chengjian Zhao
Metastasis is the primary cause of death for most cancer patients. Hematogenous arrest of circulating tumor cells (CTCs) is an essential prerequisite for metastases formation. Using transparent transgenic zebrafish (kdrl:eGFP; Casper), together with resonant laser scanning confocal microscopy, we tracked the fate of CTCs in vivo in the blood circulation for days. We found the intra-capillary morphology-switch (ICMS) of individual CTCs from strip to sphere was necessary for their intravascular arrests. Further genetic and pharmacological inhibition experiments indicated that the RhoA signaling was necessary for ICMS and the arrest of CTCs...
December 26, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/29277393/the-possible-repositioning-of-an-oral-anti-arthritic-drug-auranofin-for-nrf2-activating-therapy-the-demonstration-of-nrf2-dependent-anti-oxidative-action-using-a-zebrafish-model
#14
Yuji Fuse, Yuka Endo, Sho Araoi, Hiroaki Daitoku, Hiroyuki Suzuki, Mitsuyasu Kato, Makoto Kobayashi
The Nrf2 pathway is a biological defense system against oxidative stress. The pharmacological activation of the Nrf2 pathway is a promising therapy for oxidative stress-related diseases, but it has been challenging to find an Nrf2 activator with acceptable toxicity. To circumvent this problem, we focused on an already approved oral anti-arthritic drug, auranofin that has been reported to have the potential to activate Nrf2. We used a zebrafish model to investigate whether auranofin has protective action against oxidative stress in vivo...
December 19, 2017: Free Radical Biology & Medicine
https://www.readbyqxmd.com/read/29274064/metabolic-and-bone-effects-of-high-fat-diet-in-adult-zebrafish
#15
Marta Carnovali, Livio Luzi, Ileana Terruzzi, Giuseppe Banfi, Massimo Mariotti
An increase of visceral fat affects human bone health causing fragility, mechanical strength reduction, and increased propensity to fractures because of impaired bone matrix microstructure and aberrant bone cell function. Adult Danio rerio (zebrafish) represents a powerful model to study both metabolic diseases and bone metabolism. The aim of this study was to generate an obese adult zebrafish by high-fat diet and evaluate metabolic and bone tissue effects. Fish blood glucose and insulin levels were found to be altered in high-fat diet fish revealing a failure in β-cells insulin production...
December 22, 2017: Endocrine
https://www.readbyqxmd.com/read/29261035/zebrafish-axenic-larvae-colonization-with-human-intestinal-microbiota
#16
Nerea Arias-Jayo, Laura Alonso-Saez, Andoni Ramirez-Garcia, Miguel A Pardo
The human intestine hosts a vast and complex microbial community that is vital for maintaining several functions related with host health. The processes that determine the gut microbiome composition are poorly understood, being the interaction between species, the external environment, and the relationship with the host the most feasible. Animal models offer the opportunity to understand the interactions between the host and the microbiota. There are different gnotobiotic mice or rat models colonized with the human microbiota, however, to our knowledge, there are no reports on the colonization of germ-free zebrafish with a complex human intestinal microbiota...
December 20, 2017: Zebrafish
https://www.readbyqxmd.com/read/29258588/integrating-phenotype-ontologies-with-phenomenet
#17
Miguel Ángel Rodríguez-García, Georgios V Gkoutos, Paul N Schofield, Robert Hoehndorf
BACKGROUND: Integration and analysis of phenotype data from humans and model organisms is a key challenge in building our understanding of normal biology and pathophysiology. However, the range of phenotypes and anatomical details being captured in clinical and model organism databases presents complex problems when attempting to match classes across species and across phenotypes as diverse as behaviour and neoplasia. We have previously developed PhenomeNET, a system for disease gene prioritization that includes as one of its components an ontology designed to integrate phenotype ontologies...
December 19, 2017: Journal of Biomedical Semantics
https://www.readbyqxmd.com/read/29231925/expression-profiling-of-the-retina-of-pde6c-a-zebrafish-model-of-retinal-degeneration
#18
Liyun Zhang, Xinlian Zhang, Gaonan Zhang, Chi Pui Pang, Yuk Fai Leung, Mingzhi Zhang, Wenxuan Zhong
Retinal degeneration often affects the whole retina even though the disease-causing gene is specifically expressed in the light-sensitive photoreceptors. The molecular basis of the retinal defect can potentially be determined by gene-expression profiling of the whole retina. In this study, we measured the gene-expression profile of retinas microdissected from a zebrafish pde6cw59 (pde6c) mutant. This retinal-degeneration model not only displays cone degeneration caused by a cone-specific mutation, but also other secondary cellular changes starting from 4 days postfertilization (dpf)...
December 12, 2017: Scientific Data
https://www.readbyqxmd.com/read/29230019/metabolic-profiling-of-zebrafish-danio-rerio-embryos-by-nmr-spectroscopy-reveals-multifaceted-toxicity-of-%C3%AE-methylamino-l-alanine-bmaa
#19
Upasana Roy, Laura Conklin, Jürgen Schiller, Jörg Matysik, John P Berry, A Alia
β-methylamino-L-alanine (BMAA) has been linked to several interrelated neurodegenerative diseases. Despite considerable research, specific contributions of BMAA toxicity to neurodegenerative diseases remain to be fully resolved. In the present study, we utilized state-of-the-art high-resolution magic-angle spinning nuclear magnetic resonance (HRMAS NMR), applied to intact zebrafish (Danio rerio) embryos, as a model of vertebrate development, to elucidate changes in metabolic profiles associated with BMAA exposure...
December 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29228025/a-defect-in-the-inner-kinetochore-protein-cenpt-causes-a-new-syndrome-of-severe-growth-failure
#20
Christina Y Hung, Barbara Volkmar, James D Baker, Johann W Bauer, Emanuela Gussoni, Stefan Hainzl, Alfred Klausegger, Jose Lorenzo, Ivana Mihalek, Olaf Rittinger, Mustafa Tekin, Julia E Dallman, Olaf A Bodamer
Primordial growth failure has been linked to defects in the biology of cell division and replication. The complex processes involved in microtubule spindle formation, organization and function have emerged as a dominant patho-mechanism in these conditions. The majority of reported disease genes encode for centrosome and centriole proteins, leaving kinetochore proteins by which the spindle apparatus interacts with the chromosomes largely unaccounted for. We report a novel disease gene encoding the constitutive inner kinetochore member CENPT, which is involved in kinetochore targeting and assembly, resulting in severe growth failure in two siblings of a consanguineous family...
2017: PloS One
keyword
keyword
72617
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"