keyword
MENU ▼
Read by QxMD icon Read
search

Epithelial cell

keyword
https://www.readbyqxmd.com/read/29691634/the-ctenidium-of-the-giant-clam-tridacna-squamosa-expresses-an-ammonium-transporter-1-that-displays-light-suppressed-gene-and-protein-expression-and-may-be-involved-in-ammonia-excretion
#1
Mel V Boo, Kum C Hiong, Enan J K Goh, Celine Y L Choo, Wai P Wong, Shit F Chew, Yuen K Ip
Ammonium transporters (AMTs) can participate in ammonia uptake or excretion across the plasma membrane of prokaryotic, plant and invertebrate cells. The giant clam, Tridacna squamosa, harbors nitrogen-deficient symbiotic zooxanthellae, and normally conducts light-enhanced ammonia absorption to benefit the symbionts. Nonetheless, it can excrete ammonia when there is a supply of exogenous nitrogen or exposed to continuous darkness. This study aimed to elucidate the role of AMT1 in the ctenidium of T. squamosa by cloning and characterizing the AMT1/AMT1, determining its subcellular localization, and examining changes in its transcript and protein expression levels in response to light exposure...
April 24, 2018: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/29691598/hepatocyte-transplantation-and-advancements-in-alternative-cell-sources-for-liver-based-regenerative-medicine
#2
REVIEW
Charlotte A Lee, Siddharth Sinha, Emer Fitzpatrick, Anil Dhawan
Human hepatocyte transplantation has been actively perused as an alternative to liver replacement for acute liver failure and liver-based metabolic defects. Current challenges in this field include a limited cell source, reduced cell viability following cryopreservation and poor engraftment of cells into the recipient liver with consequent limited life span. As a result, alternative stem cell sources such as pluripotent stem cells, fibroblasts, hepatic progenitor cells, amniotic epithelial cells and mesenchymal stem/stromal cells (MSCs) can be used to generate induced hepatocyte like cells (HLC) with each technique exhibiting advantages and disadvantages...
April 24, 2018: Journal of Molecular Medicine: Official Organ of the "Gesellschaft Deutscher Naturforscher und Ärzte"
https://www.readbyqxmd.com/read/29691525/multiple-mechanisms-applied-by-lactobacillus-pentosus-at6-to-mute-the-lethal-effects-of-salmonella-in-a-mouse-model
#3
Junsheng Liu, Zhennan Gu, Wenwei Lu, Diangeng Hu, Xi Zhao, Hongxuan Huang, Hao Zhang, Jianxin Zhao, Wei Chen
Probiotics are now prevalent world-wide, as functional food supplements with many benefits for humans and animals, such as protective effects against pathogenic infection. We showed that oral supplementation of Lactobacillus pentosus AT6 (AT6) decreased the mortality rate of mice with Salmonella infection. A series of experiments showed that the protective effects of AT6 on mice involved multiple mechanisms, including (1) the inhibition of Salmonella Typhimurium growth by AT6 or its cell-free culture supernatants (CFCSs); (2) the reduction of the bacterial loads of Salmonella Typhimurium in intestinal contents and internal organs, such as the liver and spleen; (3) the inhibition of adhesion and invasion of Salmonella Typhimurium into intestinal epithelial cells; and (4) the regulation of host immunities by modifying the production of a chain of cytokines...
April 25, 2018: Food & Function
https://www.readbyqxmd.com/read/29691438/the-hippo-signaling-transducer-taz-regulates-mammary-gland-morphogenesis-and-carcinogen-induced-mammary-tumorigenesis
#4
Kayla E Denson, Ashley L Mussell, He Shen, Alexander Truskinovsky, Nuo Yang, Natesh Parashurama, Yanmin Chen, Costa Frangou, Fajun Yang, Jianmin Zhang
Hippo signaling pathway is an evolutionarily conserved pathway that controls organ size by regulating cell proliferation, apoptosis and stem cell self-renewal. TAZ (transcriptional coactivator with the PDZ-binding motif) is a key downstream effector of the mammalian Hippo pathway. Here, using a transgenic mouse model with mammary-gland-specific expression of constitutively active TAZ, we found that TAZ induction in mammary epithelial cells was associated with an increase in mammary glandular size, which probably resulted from adipocyte hypertrophy...
April 24, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29691371/helicobacter-pylori-induced-il-33-modulates-mast-cell-responses-benefits-bacterial-growth-and-contributes-to-gastritis
#5
Yi-Pin Lv, Yong-Sheng Teng, Fang-Yuan Mao, Liu-Sheng Peng, Jin-Yu Zhang, Ping Cheng, Yu-Gang Liu, Hui Kong, Ting-Ting Wang, Xiao-Long Wu, Chuan-Jie Hao, Weisan Chen, Shi-Ming Yang, Yong-Liang Zhao, Bin Han, Qiang Ma, Quan-Ming Zou, Yuan Zhuang
Interleukin (IL)-induced inflammatory responses are critical for the pathogenesis of Helicobacter pylori (H. pylori)-induced gastritis. IL-33 represents a recently discovered proinflammatory cytokine involved in inflammatory diseases, but its relevance to H. pylori-induced gastritis is unknown. Here, we found that gastric IL-33 mRNA and protein expression were elevated in gastric mucosa of both patients and mice infected with H. pylori, which is positively correlated with bacterial load and the degree of gastritis...
April 25, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29691341/epigenetic-regulation-of-tumor-suppressors-by-helicobacter-pylori-enhances-ebv-induced-proliferation-of-gastric-epithelial-cells
#6
Saurabh Pandey, Hem Chandra Jha, Sanket Kumar Shukla, Meghan K Shirley, Erle S Robertson
Helicobacter pylori and Epstein-Barr virus (EBV) are two well-known contributors to cancer and can establish lifelong persistent infection in the host. This leads to chronic inflammation, which also contributes to development of cancer. Association with H. pylori increases the risk of gastric carcinoma, and coexistence with EBV enhances proliferation of infected cells. Further, H. pylori -EBV coinfection causes chronic inflammation in pediatric patients. We have established an H. pylori -EBV coinfection model system using human gastric epithelial cells...
April 24, 2018: MBio
https://www.readbyqxmd.com/read/29691225/suppression-of-epithelial-folding-at-actomyosin-enriched-compartment-boundaries-downstream-of-wingless-signalling-in-drosophila
#7
Jose M Urbano, Huw W Naylor, Elena Scarpa, Leila Muresan, Bénédicte Sanson
Epithelial folding shapes embryos and tissues during development. Here, we investigate the coupling between epithelial folding and actomyosin-enriched compartmental boundaries. The mechanistic relationship between the two is unclear, because actomyosin-enriched boundaries are not necessarily associated with folds. Also, some cases of epithelial folding occur independently of actomyosin contractility. We investigated the shallow folds called parasegment grooves that form at boundaries between anterior and posterior compartments in the early Drosophila embryo...
April 24, 2018: Development
https://www.readbyqxmd.com/read/29691018/carboxymethyl-chitosan-gelatin-hyaluronic-acid-blended-membranes-as-epithelia-transplanting-scaffold-for-corneal-wound-healing
#8
Wenhua Xu, Zheying Wang, Ying Liu, Liping Wang, Zhiwen Jiang, Tong Li, Wenhua Zhang, Ye Liang
There is an unmet need for an optimal scaffold as cell transplantation carrier to induce corneal reconstruction. In this study, a blend membrane was prepared with carboxymethyl chitosan (CMCTS), gelatin, and hyaluronic acid. To investigate its cytocompatibility, primary rabbit corneal epithelial cells (CEpCs) were seeded on it and growth and proliferation were evaluated. The blend membrane was found to be transparent, biodegradable, and suitable for CEpCs attachment and proliferation, which could maintain the epithelial cell-like protein expression of CEpCs...
July 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29690953/stat3-regulates-liver-progenitor-cell-driven-liver-regeneration-in-zebrafish
#9
Mehwish Khaliq, Sungjin Ko, Yinzi Liu, Hualin Wang, Yonghua Sun, Lila Solnica-Kreze, Donghun Shin
After liver injury, regeneration manifests as either (1) hepatocytes proliferating to restore the lost hepatocyte massor (2) if hepatocyte proliferation is compromised, biliary epithelial cells (BECs) dedifferentiating into liver progenitor cells (LPCs), which subsequently differentiate into hepatocytes. Following pharmacogenetic ablation of hepatocytes in Tg(fabp10a:CFP-NTR) zebrafish, resulting in severe liver injury, signal transducer and activator of transcription 3 (Stat3) and its target gene and negative regulator, socs3a , were upregulated in regenerating livers...
April 24, 2018: Gene Expression
https://www.readbyqxmd.com/read/29690905/resolution-of-bleomycin-induced-murine-pulmonary-fibrosis-via-a-splenic-lymphocyte-subpopulation
#10
Koichiro Kamio, Arata Azuma, Kuniko Matsuda, Jiro Usuki, Minoru Inomata, Akemi Morinaga, Takeru Kashiwada, Nobuhiko Nishijima, Shioto Itakura, Nariaki Kokuho, Kenichiro Atsumi, Hiroki Hayashi, Tomoyoshi Yamaguchi, Kazue Fujita, Yoshinobu Saito, Shinji Abe, Kaoru Kubota, Akihiko Gemma
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a progressive disease with high mortality, and the pathogenesis of the disease is still incompletely understood. Although lymphocytes, especially CD4+ CD25+ FoxP3+ regulatory T cells (Tregs), have been implicated in the development of IPF, contradictory results have been reported regarding the contribution of Tregs to fibrosis both in animals and humans. The aim of this study was to investigate whether a specific T cell subset has therapeutic potential in inhibiting bleomycin (BLM)-induced murine pulmonary fibrosis...
April 24, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29690747/tumor-associated-macrophages-derived-tgf-%C3%AE-%C3%A2-induced-epithelial-to-mesenchymal-transition-in-colorectal-cancer-cells-through-smad2-3-4-snail-signaling-pathway
#11
Jianhui Cai, Limin Xia, Jinlei Li, Shichang Ni, Huayu Song, Xiangbin Wu
Purpose: We investigated the role of tumor-associated macrophages (TAMs) on the epithelial to mesenchymal transition (EMT) of colorectal cancer cells and determined the potential mechanism involved in the metastatic process. Materials and Methods: In this study, flow cytometry was used to detect the expression of target proteins. We used transwell assay to evaluate the migration of cancer cells under specific conditions. Using real-time polymerase chain reaction, we examined the expressions of cytokines and EMT-related markers in mRNA level...
April 25, 2018: Cancer Research and Treatment: Official Journal of Korean Cancer Association
https://www.readbyqxmd.com/read/29690739/-effects-of-short-chain-fatty-acid-on-barrier-disruption-of-human-intestinal-epithelial-cell-induced-by-endotoxin-lipopolysaccharide-and-the-related-mechanism
#12
Y H Feng, Y L Huang, P Wang, F J Wang
Objective: To investigate the effects of short chain fatty acid (SCFA) on barrier disruption of human intestinal epithelial cell induced by endotoxin/lipopolysaccharide (LPS) and the related mechanism. Methods: The human intestinal epithelial cell line Caco-2 was used to reproduce monolayer-cells. Cells were divided into control group, LPS group, and SCFA+ LPS group according to the random number table. Cells in control group were only routinely cultured with DMEM medium. Cells in LPS group were cultured with DMEM medium and LPS with final mass concentration of 10 μg/mL...
April 20, 2018: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/29690696/-expression-and-potential-clinical-significance-of-cytidine-triphosphate-phosphocholine-cytidylyltransferase-%C3%AE-in-oral-squamous-cell-carcinoma
#13
F C Lu, X Y Xie, X M Yin, Y J Gao
Objective: To examine the expression and potential clinical significance of CCT (cytidine triphosphate: phosphocholine cytidylyltransferase)-α in oral squamous cell carcinoma (OSCC). Methods: Fifty-eight OSCC and paired adjacent non-malignant epithelia samples (between May 2016 and July 2016) were obtained from dental center, Second Xiangya Hospital, Central South University. CCT-α expression was examined by immunohistochemistry. The relationship between CCT-α and clinicopathological features of OSCC patients was analyzed...
April 9, 2018: Zhonghua Kou Qiang Yi Xue za Zhi, Zhonghua Kouqiang Yixue Zazhi, Chinese Journal of Stomatology
https://www.readbyqxmd.com/read/29690640/non-canonical-activation-of-the-epidermal-growth-factor-receptor-by-carbon-nanoparticles
#14
Daniel Stöckmann, Tim Spannbrucker, Niloofar Ale-Agha, Philipp Jakobs, Christine Goy, Nadine Dyballa-Rukes, Tamara Hornstein, Alexander Kümper, Annette Kraegeloh, Judith Haendeler, Klaus Unfried
The epidermal growth factor receptor (EGFR) is an abundant membrane protein, which is essential for regulating many cellular processes including cell proliferation. In our earlier studies, we observed an activation of the EGFR and subsequent signaling events after the exposure of epithelial cells to carbon nanoparticles. In the current study, we describe molecular mechanisms that allow for discriminating carbon nanoparticle-specific from ligand-dependent receptor activation. Caveolin-1 is a key player that co-localizes with the EGFR upon receptor activation by carbon nanoparticles...
April 23, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29690584/novel-inhibitor-discovery-of-staphylococcus-aureus-sortase-b-and-the-mechanism-confirmation-via-molecular-modeling
#15
Guizhen Wang, Xiyan Wang, Lin Sun, Yawen Gao, Xiaodi Niu, Hongsu Wang
SortaseB (SrtB) plays a critical role in Staphylococcus aureus ( S. aureus ) infections. According to the reports in the literature, SrtB can anchor the IsdC to the cell wall to capture iron from the host to achieve a successful invasion. On the other hand, SrtB could also affect the adhesion of S. aureus to host cells based on previous studies. Here, we report about a novel SrtB inhibitor, coptisine, a natural compound that does not exhibit antibacterial activity but can inhibit the SrtB activity in vitro...
April 23, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29689643/il-17a-promotes-the-invasion-metastasis-cascade-via-the-akt-pathway-in-hepatocellular-carcinoma
#16
Qing-Guo Xu, Jian Yu, Xing-Gang Guo, Guo-Jun Hou, Sheng-Xian Yuan, Yuan Yang, Yun Yang, Hui Liu, Ze-Ya Pan, Fu Yang, Fang-Ming Gu, Wei-Ping Zhou
We previously demonstrated that interleukin-17A (IL-17A) is associated with the progression of hepatocellular carcinoma (HCC). However, its role in the invasion-metastasis cascade of HCC and the efficacy of IL-17A-targeting therapeutics in HCC remain largely unknown. In this study, we found that IL-17A promoted intrahepatic and pulmonary metastasis of HCC cells in an orthotopic implant model. Moreover, our results showed that IL-17A induced epithelial-mesenchymal transition (EMT) and promoted HCC cell colonization in vitro and in vivo, and the role of IL-17A in invasion-metastasis was dependent on activation of the AKT pathway...
April 24, 2018: Molecular Oncology
https://www.readbyqxmd.com/read/29689570/major-differences-in-hypoxia-tolerance-and-p38-regulation-among-different-renal-cells
#17
Qianqian Shi, Jian Shi, Fengbao Luo, Guanglai Song, Xiaozhou He, Ying Xia
BACKGROUND/AIMS: Mitogen-activated protein kinases (MAPKs) are involved in the cellular response to hypoxia and their dysregulation may contribute to the progression and pathology of diverse human renal diseases. Recent studies suggest that the regulation of MAPK responses to hypoxic stress may be different in different cells, even within the same organ. However, it is unclear if MAPKs are differentially regulated in different renal cells in hypoxia. This work was carried out to clarify this fundamental issue...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29689552/rbfox3-regulates-the-chemosensitivity-of-cancer-cells-to-5-fluorouracil-via-the-pi3k-akt-emt-and-cytochrome-c-caspase-pathways
#18
Tianze Liu, Xiaojun Wu, Yizhuo Li, Wenjing Lu, Fufu Zheng, Changlin Zhang, Qian Long, Huijuan Qiu, Yixin Li, Qin Ge, Miao Chen, Xinfa Yu, Wangbing Chen, Hongyang Zhang, Wenlin Huang, Meihua Luo, Wuguo Deng, Liren Li
BACKGROUND/AIMS: RBFOX3, an RNA-binding fox protein, plays an important role in the differentiation of neuronal development, but its role in the chemosensitivity of hepatocellular carcinoma (HCC) to 5-FU is unknown. METHODS: In this study, we examined the biological functions of RBFOX3 and its effect on the chemosensitivity of HCC cells to 5-FU in vitro and in a mouse xenograft model. RESULTS: RBFOX3 was found to have elevated expression in HCC cell lines and tissue samples, and its knockdown inhibited HCC cell proliferation...
April 18, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29689522/differential-effects-of-lipopolysaccharide-on-mouse-sensory-trp-channels
#19
Brett Boonen, Yeranddy A Alpizar, Alicia Sanchez, Alejandro López-Requena, Thomas Voets, Karel Talavera
Acute neurogenic inflammation and pain associated to bacterial infection have been traditionally ascribed to sensitization and activation of sensory nerve afferents secondary to immune cell stimulation. However, we recently showed that lipopolysaccharides (LPS) directly activate the Transient Receptor Potential channels TRPA1 in sensory neurons and TRPV4 in airway epithelial cells. Here we investigated whether LPS activates other sensory TRP channels expressed in sensory neurons. Using intracellular Ca2+ imaging and patch-clamp we determined the effects of LPS on recombinant TRPV1, TRPV2, TRPM3 and TRPM8, heterologously expressed in HEK293T cells...
April 14, 2018: Cell Calcium
https://www.readbyqxmd.com/read/29689462/macaque-homologs-of-kaposi-s-sarcoma-associated-herpesvirus-kshv-infect-germinal-center-lymphoid-cells-epithelial-cells-in-skin-and-gastrointestinal-tract-and-gonadal-germ-cells-in-naturally-infected-macaques
#20
Helle Bielefeldt-Ohmann, A Gregory Bruce, Kellie Howard, Minako Ikoma, Margaret E Thouless, Timothy M Rose
We developed a set of rabbit antisera to characterize infections by the macaque RV2 rhadinovirus homologs of KSHV. We analyzed tissues from rhesus and pig-tailed macaques naturally infected with rhesus rhadinovirus (RRV) or Macaca nemestrina rhadinovirus 2 (MneRV2). Our study demonstrates that RV2 rhadinoviruses have a tropism for epithelial cells, lymphocytes and gonadal germ cells in vivo. We observed latent infections in both undifferentiated and differentiated epithelial cells with expression of the latency marker, LANA...
April 21, 2018: Virology
keyword
keyword
72598
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"