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https://www.readbyqxmd.com/read/27903094/effects-of-self-complementarity-codon-optimization-transgene-and-dose-on-liver-transduction-with-aav8
#1
Peter Bell, Lili Wang, Shu-Jen Chen, Hongwei Yu, Yanqing Zhu, Mohamad Nayal, Zhenning He, John White, Deborah Lebel-Hagan, James M Wilson
Numerous methods of vector design and delivery have been employed in an attempt to increase transgene expression following AAV-based gene therapy. Here, we conducted a gene transfer study in mice to compare the effects of vector self-complementarity (double- or single-stranded DNA), codon optimization of the transgene, and vector dose on transgene expression levels in liver. Two different reporter genes were used: human ornithine transcarbamylase (hOTC) detected by immunofluorescence, and enhanced green fluorescent protein (EGFP) detected by direct fluorescence...
November 30, 2016: Human Gene Therapy Methods
https://www.readbyqxmd.com/read/27902298/description-of-pseudactinotalea-terrae-gen-nov-sp-nov-isolated-from-greenhouse-soil-and-proposal-to-reclassify-actinotlea-suaedae-as-pseudactinotalea-suaedae-comb-nov
#2
Hayoung Cho, Moriyuki Hamada, Jae-Hyung Ahn, Hang-Yeon Weon, Jae-Ho Joa, Ken-Ichiro Suzuki, Soon-Wo Kwon, Soo-Jin Kim
One bacterial strain, designated 5GHs33-3T, was isolated from greenhouse soil collected from Yongin region, Gyeonggi province, South Korea. It was aerobic, Gram-stain-positive, flagellated, rod-shaped bacterium. The strain grew at the range of 4-37 °C (optimally 28-30 °C), pH 6.0-10.0 (optimally pH 7.0) and 0-7 % (w/v) NaCl concentrations (optimally, 0 %). The 16S rRNA gene sequence analysis revealed that it had high sequence similarity with Actinotalea suaedae EGI 60002T (98.4 %), Actinotalea ferrariae CF5-4T (96...
November 30, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27902249/microbacterium-sorbitolivorans-sp-nov-a-novel-member-of-microbacteriaceae-isolated-from-fermentingbed-in-pigpen
#3
Man Cai, Yu-Chen Meng, Hong-Can Liu, Lei-Lei Yang, Ying-Qian Kang, Yu-Guang Zhou
Two bacterial strains SZDIS-1-1T and GZDIS-1-1 were isolated from fermentingbed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short-rods without flagellum. Their nearest phylogenetic neighbors were Microbacterium amylolyticum N5T (16S rRNA gene sequences similarity 98.2 %), Microbacterium indicum BBH6T (97.9 %) and Microbacterium gubbeenense DPC 5286T (97.8 %) with DNA-DNA hybridization values to strain SZDIS-1-1T as 20.0±1.2 %, 14.3±5.8 % and 19.1±1.6 %, respectively...
October 9, 2016: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/27898998/nutritional-supplementation-for-hip-fracture-aftercare-in-older-people
#4
REVIEW
Alison Avenell, Toby O Smith, James P Curtain, Jenson Cs Mak, Phyo K Myint
BACKGROUND: Older people with hip fractures are often malnourished at the time of fracture, and subsequently have poor food intake. This is an update of a Cochrane review first published in 2000, and previously updated in 2010. OBJECTIVES: To review the effects (benefits and harms) of nutritional interventions in older people recovering from hip fracture. SEARCH METHODS: We searched the Cochrane Bone, Joint and Muscle Trauma Group Specialised Register, CENTRAL, MEDLINE, MEDLINE In-Process & Other Non-Indexed Citations, Embase, CAB Abstracts, CINAHL, trial registers and reference lists...
November 30, 2016: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/27891735/hyperammonemia-in-ornithine-transcarbamylase-deficient-recipients-following-living-donor-liver-transplantation-from-heterozygous-carrier-donors
#5
Tri Hening Rahayatri, Hajime Uchida, Kengo Sasaki, Takanobu Shigeta, Yoshihiro Hirata, Hiroyuki Kanazawa, Vidyadhar Mali, Akinari Fukuda, Seisuke Sakamoto, Mureo Kasahara
Ornithine transcarbamylase deficiency (OTCD) is a urea cycle disorder of X-linked inheritance, affecting the detoxification of excess nitrogen and leading to hyperammonemia (hyper-NH3 ). Living donor liver transplantation (LDLT) has been applied for the treatment of OTCD. This case series retrospectively reviewed two OTCD patients who experienced hyper-NH3 following LDLT. The first case was a 5-year-old girl who had onset of OTCD at 2 years of age. Ornithine transcarbamylase (OTC) enzyme activity was 62% for the donor and 15% for the recipient...
November 28, 2016: Pediatric Transplantation
https://www.readbyqxmd.com/read/27890695/nitric-oxide-polyamines-cross-talk-during-dormancy-release-and-germination-of-apple-embryos
#6
Urszula Krasuska, Katarzyna Ciacka, Agnieszka Gniazdowska
Nitric oxide (NO) and polyamines (PAs) belong to plant growth and development regulators. These compounds play a key role in numerous physiological processes e.g. seed germination. Based on the suggestion of overlapping of NO and PAs biosynthetic pathways, we demonstrated a cross-talk of NO and PAs in regulation of embryonic dormancy release. The aim of the work was to investigate an impact of PAs (Put, Spd and Spm) or NO short-term fumigation on nitrite, urea, Arg and ornithine (Orn) content, NO synthase-like (NOS-like) and arginase activity in axes of apple (Malus domestica Borkh...
November 23, 2016: Nitric Oxide: Biology and Chemistry
https://www.readbyqxmd.com/read/27885170/elucidation-of-the-polyamine-biosynthesis-pathway-during-brazilian-pine-araucaria-angustifolia-seed-development
#7
Leandro F de Oliveira, Paula Elbl, Bruno V Navarro, Amanda F Macedo, André L W Dos Santos, Eny I S Floh
Polyamines (PAs), such as spermidine and spermine, as well as amino acids that are substrates for their biosynthesis, are known to be essential for plant development. However, little is known about the gene expression and metabolic switches associated with the ornithine/arginine and PA biosynthetic pathway during seed development in conifers. To understand these metabolic switches, the enzyme activity of arginine decarboxylase and ornithine decarboxylase, as well as the contents of PAs and amino acids were evaluated in three Araucaria angustifolia (Bertol...
November 23, 2016: Tree Physiology
https://www.readbyqxmd.com/read/27880890/potential-antidepressant-and-resilience-mechanism-revealed-by-metabolomic-study-on-peripheral-blood-mononuclear-cells-of-stress-resilient-rats
#8
Juan Li, Shu-Xiao Zhang, Wei Wang, Ke Cheng, Hua Guo, Cheng-Long Rao, De-Yu Yang, Yong He, De-Zhi Zou, Yu Han, Li-Bo Zhao, Peng-Fei Li, Peng Xie
Resilience is an active coping response to stress, which plays a very important role in major depressive disorder study. The molecular mechanisms underlying such resilience are poorly understood. Peripheral blood mononuclear cells (PBMCs) were promising objects in unveiling the underlying pathogenesis of resilience. Hereby we carried out successive study on PBMCs metabolomics in resilient rats of chronic unpredictable mild stress (CUMS) model. A gas chromatography-mass spectrometry (GC-MS) metabolomic approach coupled with principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) was used to detect differential metabolites in PBMCs of resilient rats...
November 20, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27876630/disruptions-of-the-genes-involved-in-lysine-biosynthesis-iron-acquisition-and-secondary-metabolisms-affect-virulence-and-fitness-in-metarhizium-robertsii
#9
Bruno Giuliano Garisto Donzelli, B Gillian Turgeon, Donna M Gibson, Stuart B Krasnoff
Based on genomic analysis, polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) pathways account for biosynthesis of the majority of the secondary metabolites produced by the entomopathogenic fungus Metarhizium robertsii. To evaluate the contribution of these pathways to M. robertsii fitness and/or virulence, mutants deleted for mrpptA, the Sfp-type 4' phosphopantetheinyl transferase gene required for their activation were generated. ΔmrpptA strains were deficient in PKS and NRPS activity resulting in colonies lacking the typical green pigment that failed to produce the nonribosomal peptides destruxins, serinocylins, and the siderophores ferricrocin and metachelins, as well as the hybrid polyketide-peptides NG-39x that are all produced by the wild type (WT) M...
November 19, 2016: Fungal Genetics and Biology: FG & B
https://www.readbyqxmd.com/read/27874083/poly-l-ornithine-enhances-migration-of-neural-stem-progenitor-cells-via-promoting-%C3%AE-actinin-4-binding-to-actin-filaments
#10
Hongfei Ge, Anyong Yu, Jingyu Chen, Jichao Yuan, Yi Yin, Wangsheng Duanmu, Liang Tan, Yang Yang, Chuan Lan, Weixiang Chen, Hua Feng, Rong Hu
The recruitment of neural stem/progenitor cells (NSPCs) for brain restoration after injury is a promising regenerative therapeutic strategy. This strategy involves enhancing proliferation, migration and neuronal differentation of NSPCs. To date, the lack of biomaterials, which facilitate these processes to enhance neural regeneration, is an obstacle for the cell replacement therapies. Our previous study has shown that NSPCs grown on poly-L-ornithine (PO) could proliferate more vigorously and differentiate into more neurons than that on Poly-L-Lysine (PLL) and Fibronectin (FN)...
November 22, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27870265/antizyme-inhibitor-1-a-potential-carcinogenic-molecule
#11
REVIEW
Shiqiao Qiu, Jing Liu, Feiyue Xing
Polyamines are multivalent and organic cations essential for cellular growth, proliferation, differentiation and apoptosis. Increased levels of polyamines are closely associated with numerous forms of cancer. An autoregulatory circuit composed by the ornithine decarboxylase(ODC), antizymes(AZ) and antizyme inhibitor (AZI) governs the intracellular level of polyamines. AZ binds with ODC to inhibit the ODC activity and to promote the ubiquitin-independent degradation of ODC. AZI binds to AZ with a higher affinity than ODC...
November 21, 2016: Cancer Science
https://www.readbyqxmd.com/read/27869536/comparative-analysis-of-nonvolatile-and-volatile-metabolites-in-lichtheimia-ramosa-cultivated-in-different-growth-media
#12
Hyun Chung, NaKyeom Lee, Jeong-Ah Seo, Young-Suk Kim
Lichtheimia ramosa is one of the predominant filamentous fungi in Korean traditional nuruk. The nonvolatile and volatile metabolites of L. ramosa cultivated in three growth media: complete medium (CM), potato dextrose broth (PDB), and sabouraud dextrose broth (SDB), were investigated and compared. Among nonvolatile metabolites, serine, lysine, and ornithine increased in CM and PDB cultivated with L. ramosa during the exponential phase. In addition, glucose level increased in CM whereas decreased in PDB and SDB...
November 21, 2016: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/27862081/arginase-inhibitors-a-rational-approach-over-one-century
#13
REVIEW
Marc Pudlo, Céline Demougeot, Corine Girard-Thernier
Arginase (EC 3.5.3.1) is the bimanganese enzyme that converts L-arginine into ornithine and urea. This enzyme was discovered more than a century ago and early α-amino acids were identified as weak inhibitors. It was only during the 90s, after nitric oxide (NO) was reported as one of the most important biological mediators and when tight interrelation of arginase and NO synthase was found, that the development of arginase inhibitors was accelerated. The regulation of arginase activity by the N-hydroxy-L-arginine (3, NOHA) intermediate of the NO synthesis was the starting point of the N-hydroxy-nor-arginine (21, nor-NOHA) that proved to be the first micromolar inhibitor...
November 15, 2016: Medicinal Research Reviews
https://www.readbyqxmd.com/read/27832417/lethal-neonatal-hyperammonemia-in-severe-ornithine-transcarbamylase-otc-deficiency-compounded-by-large-hepatic-portosystemic-shunt
#14
Lauren Beard, Erica Wymore, Laura Fenton, Curtis R Coughlin, James D Weisfeld-Adams
No abstract text is available yet for this article.
November 10, 2016: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/27830380/recovery-of-pan-genotypic-and-genotype-specific-amino-acid-alterations-in-chronic-hepatitis-c-after-viral-clearance-transition-at-the-crossroad-of-metabolism-and-immunity
#15
Ming-Ling Chang, Mei-Ling Cheng, Su-Wei Chang, Hsiang-Yu Tang, Cheng-Tang Chiu, Chau-Ting Yeh, Ming-Shi Shiao
Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched male genotype 1 (G1) and G2 patients underwent paired serum metabolomics analyses with liquid chromatography-tandem mass spectrometry to examine AAs before and 24 weeks after anti-HCV therapy. Before anti-HCV therapy, G1 patients had a higher HCV RNA level than G2 patients...
November 10, 2016: Amino Acids
https://www.readbyqxmd.com/read/27819481/characterization-of-klebsiella-sp-strain-s1-a-bacterial-producer-of-secoisolariciresinol-through-biotransformation
#16
Yu-Jie Zhou, Songling Zhu, Dong-Hui Yang, Dan-Dan Zhao, Jia-Jing Li, Shu-Lin Liu
Secoisolariciresinol (SECO) is a lignan of potential therapeutic value for diseases such as cancer, but its use has been limited by the lack of ideal production methods, even though its precursors are abundant in plants, such as flaxseeds. Here, we report the characterization of a bacterial strain, S1, isolated from the human intestinal flora, which could produce secoisolariciresinol by biotransformation of precursors in defatted flaxseeds. This bacterium was a Gram-negative and facultatively anaerobic straight rod without capsules...
June 29, 2016: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/27808470/stable-production-of-cyanophycinase-in-nicotiana-benthamiana-and-its-functionality-to-hydrolyse-cyanophycin-in-the-murine-intestine
#17
Daniel Ponndorf, Sven Ehmke, Benjamin Walliser, Kerstin Thoss, Christoph Unger, Solvig Görs, Gürbüz Daş, Cornelia C Metges, Inge Broer, Henrik Nausch
Food supplementation with the conditionally essential amino acid arginine (Arg) has been shown to have nutritional benefits. Degradation of cyanophycin (CGP), a peptide polymer used for nitrogen storage by cyanobacteria, requires cyanophycinase (CGPase) and results in the release of β-aspartic acid (Asp)-(Arg) dipeptides. The simultaneous production of CGP and CGPase in plants could be a convenient source of Arg dipeptides. Different variants of the cphB coding region from Thermosynechococcus elongatus BP-1 were transiently expressed in Nicotiana benthamiana plants...
November 3, 2016: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/27808157/n-acetyl-ornithine-deacetylase-is-a-moonlighting-protein-and-is-involved-in-the-adaptation-of-entamoeba-histolytica-to-nitrosative-stress
#18
Preeti Shahi, Meirav Trebicz-Geffen, Shruti Nagaraja, Rivka Hertz, Sharon Alterzon-Baumel, Karen Methling, Michael Lalk, Mohit Mazumder, Gourinath Samudrala, Serge Ankri
Adaptation of the Entamoeba histolytica parasite to toxic levels of nitric oxide (NO) that are produced by phagocytes may be essential for the establishment of chronic amebiasis and the parasite's survival in its host. In order to obtain insight into the mechanism of E. histolytica's adaptation to NO, E. histolytica trophozoites were progressively adapted to increasing concentrations of the NO donor drug, S-nitrosoglutathione (GSNO) up to a concentration of 110 μM. The transcriptome of NO adapted trophozoites (NAT) was investigated by RNA sequencing (RNA-seq)...
November 3, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27804281/convergent-evolution-of-the-arginine-deiminase-pathway-the-arcd-and-arce-arginine-ornithine-exchangers
#19
Elke E E Noens, Juke S Lolkema
The arginine deiminase (ADI) pathway converts L-arginine into L-ornithine and yields 1 mol of ATP per mol of L-arginine consumed. The L-arginine/L-ornithine exchanger in the pathway takes up L-arginine and excretes L-ornithine from the cytoplasm. Analysis of the genomes of 1281 bacterial species revealed the presence of 124 arc gene clusters encoding the pathway. About half of the clusters contained the gene encoding the well-studied L-arginine/L-ornithine exchanger ArcD, while the other half contained a gene, termed here arcE, encoding a membrane protein that is not a homolog of ArcD...
November 1, 2016: MicrobiologyOpen
https://www.readbyqxmd.com/read/27803737/impact-of-ornithine-phenylacetate-ocr-002-in-lowering-plasma-ammonia-after-upper-gastrointestinal-bleeding-in-cirrhotic-patients
#20
Meritxell Ventura-Cots, Mar Concepción, José Antonio Arranz, Macarena Simón-Talero, Maria Torrens, Albert Blanco-Grau, Inma Fuentes, Pilar Suñé, Edilmar Alvarado-Tapias, Cristina Gely, Eva Roman, Beatriz Mínguez, German Soriano, Joan Genescà, Juan Córdoba
BACKGROUND: Ornithine phenylacetate (OP) has been proven effective in lowering ammonia plasma levels in animals, and to be well tolerated in cirrhotic patients. A trial to assess OP efficacy in lowering plasma ammonia levels versus placebo in cirrhotic patients after an upper gastrointestinal bleeding was performed. The primary outcome was a decrease in venous plasma ammonia at 24 hours. METHODS: A total of 38 consecutive cirrhotic patients were enrolled within 24 hours of an upper gastrointestinal bleed...
November 2016: Therapeutic Advances in Gastroenterology
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